Melatonin

Catalog No.S1204

Melatonin Chemical Structure

Molecular Weight(MW): 232.28

Melatonin is a MT receptor agonist, used as a dietary supplement.

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In DMSO USD 90 In stock
USD 97 In stock
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Cited by 6 Publications

4 Customer Reviews

  • TUNEL staining of treated adipocytes and flow cytometry analysis of positive TUNEL cells (n=3).

    J Pineal Res. 2017, 62(4), doi: 10.1111/jpi.12383. Melatonin purchased from Selleck.

    C: EDU staining of adipocytes treated with MT for 24 h (n=3). D: Flow Cytometry analysis of cell cycle in adipocytes treated with MT for 24 h (n=3).

    J Pineal Res, 2016, doi: 10.1111/jpi.12383.. Melatonin purchased from Selleck.

  • H2O2 activates JNK through the RAC1/MAP2K7 pathway, which is inhibited by melatonin treatment. (A) GCs grown in medium containing 10 μM melatonin for 24 h were rinsed using PBS, and then incubated with 0.2 mM H2O2 for 2 h. For the inhibition of RAC1, the RAC1 specific antagonist NSC 23766 (10 μM) was added 1 h prior to H2O2 exposure. The cell lysates were collected for GST-PAK1-PBD pulldown assay of RAC1 activation (GTP-bound RAC1 levels) and immunoblotting analysis of total RAC1. (B) Western blot analysis of phosphorylated MAP2K7 (p-MAP2K7) and total MAP2K7 in GCs with the indicated treatments as described above. TUBA1A served as the control for loading. (C) The detection of JNK activity in GCs subjected to the treatments as mentioned earlier. Data represent mean ± s.e.; n = 3. **Represents P < 0.01 vs control group. ##Represents P < 0.01 vs H2O2 group. N, not significant, P > 0.05. (D) GCs transfected with scrambled control siRNA (SC siRNA) or Map2k7 siRNA were cultured with 10 μM melatonin for 24 h, washed in PBS, and then grown in medium containing 0.2 mM H2O2 for 2 h. The expression of phosphorylated MAP2K7 (p-MAP2K7) and total MAP2K7 was determined by Western blotting. TUBA1A served as the control for loading. (E) GCs transfected with scrambled control siRNA (SC siRNA) or Map2k7 siRNA for 48 h were cultured with or without 0.2 mM H2O2 for 2 h. Cell lysates were collected for Western blot analysis of RAC1 activation (GTP-RAC1 levels) by GST-PAK1-PBD pulldown and of total RAC1 levels. (F) GCs transfected with scrambled control siRNA (SC siRNA) or Map2k7 siRNA for 24 h were cultured for another 24 h in the presence or absence of 10 μM melatonin before 2 h of H2O2 (0.2 mM) incubation. Cells were then processed for JNK determination. Data represent mean ± s.e.; n = 3. **Represents P < 0.01 vs control group. ##Represents P < 0.01 vs H2O2 group. N, not significant, P > 0.05.

    Reproduction, 2018, 155(3):307-319. Melatonin purchased from Selleck.

    (a-c) Immunohistochemical detection of Nrf2, HO-1 and NQO1 in lumber enlargement of mice from different groups at the peak stage of EAE or on day 28 post immunization.

    J Mol Neurosci, 2018, 64(2):233-241. Melatonin purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Melatonin is a MT receptor agonist, used as a dietary supplement.
Targets
melatonin receptor [1]
()
In vitro

Melatonin interacts with the highly toxic hydroxyl radical with a rate constant equivalent to that of other highly efficient hydroxyl radical scavengers. Melatonin reportedly neutralizes hydrogen peroxide, singlet oxygen, peroxynitrite anion, nitric oxide and hypochlorous acid. [1] Melatonin is believed to scavenge the highly toxic hydroxyl radical, the peroxynitrite anion, and possibly the peroxyl radical. Melatonin reportedly scavenges the superoxide anion radical and it quenches singlet oxygen. Melatonin stimulates mRNA levels for superoxide dismutase and the activities of glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase (all of which are antioxidative enzymes), thereby increasing its antioxidative capacity. [2] Melatonin in cell-free systems has been shown to directly scavenge H2O2, singlet oxygen (1O2) and nitric oxide (NO*), with little or no ability to scavenge the superoxide anion radical (O2*-) in vitro. Melatonin also directly detoxifies the peroxynitrite anion (ONOO-) and/or peroxynitrous acid (ONOOH), or the activated form of this molecule, ONOOH*. Melatonin acts as a direct free radical scavenger with the ability to detoxify both reactive oxygen and reactive nitrogen species. [3] Melatonin inhibits cAMP accumulation in most of the cells examined, but the indole effects on other messengers have been often observed only in one type of the cells or tissue, until now. Melatonin also regulates the transcription factors, namely, phosphorylation of cAMP-responsive element binding protein and expression of c-Fos. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells M3u4cGZ2dmO2aX;uJIF{e2G7 NHnh[YNDcW6maX7nJIFn\mmwaYT5JIZweiCqdX3hckBu\WyjdH;ubY4hemWlZYD0c5IhfHmyZTCxRUwh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{ApOi2dMUK1TX1qd2SxbXXsZZRwdmmwIHnzJJV{\WRiYYOgdoFlcW:uaXfhcoQqNCCNaU2wMlIhdk1? NXL5W5ExOTFyNkO2NFI>
NIH 3T3 cells M3:zd2Z2dmO2aX;uJIF{e2G7 M3jndmJqdmSrbnegZYZncW6rdImgeI94[XKmczDy[YNwdWKrbnHueEBpfW2jbjDt[YxifG:waX6gdoVk\XC2b4KgeJlx\SBzQTDlfJBz\XO|ZXSgbY4hVkmKIEPUN{Bk\WyuczD1d4lv\yB{LWuxNlFKZWmxZH;t[YxifG:waX6gdoFlcW:uaXfhcoQh[mmwZHnu[{Bie3OjeTygT4k:OC54NjDuUS=> M3;ZNVExPzN5N{O4
NIH3T3 cells M4DVOGZ2dmO2aX;uJIF{e2G7 M{PRe2JqdmSrbnegZYZncW6rdImgZYdicW6|dDDoeY1idiCPVEGgcYVt[XSxbnnuJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCQSVizWFMh[2WubIOsJGtqRTBwNDDuUS=> MVuxOFY1OzN|MB?=
CHO cells M3HNR2Z2dmO2aX;uJIF{e2G7 MnroTY5pcWKrdHnvckBw\iC2aHWgNk1cOTJ3SW2tJIlw\G:vZXzheI9vcW5iYnnu[Ilv\yC2bzDN[YxifG:waX6gdoVk\XC2b4KgeJlx\SBzQTDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCNaU24MlAxTS1yNTFOwG0> MWO5OFM2QDly
NIH3T3 cells NVTtXlI3TnWwY4Tpc44h[XO|YYm= NI\pTWhDcW6maX7nJIFn\mmwaYT5JIFo[Wmwc4SgbJVu[W5iTWSyJI1mdGG2b37pckBz\WOncITvdkBmgHC{ZYPz[YQhcW5iTlnIN3Q{KGOnbHzzMEBMcT1yLkOgcm0> NHHM[XAyPDZ2M{OzNC=>
CHO-Galpha16 cells NHO4S2JHfW6ldHnvckBie3OjeR?= M3u2VFIxKG2rboO= MV\CbY5lcW6pIHHm[olvcXS7IITvJJJifCCPVEGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTz3HZYxxcGFzNjDj[YxteyCjc4Pld5Nm\CCjczDDZVIsKG2xYnnsbZpifGmxbjDh[pRmeiB{MDDtbY5{KGK7IF\MTXBTKGG|c3H5MEBGSzVyPUCuOVYhdk1? NYC1XHZqOjF{M{e2OFQ>
NIH3T3 cells NUDEd5lNTnWwY4Tpc44h[XO|YYm= MkGyPVAhdWmwcx?= MUnEbZNxdGGlZX3lcpQhd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIZzd21iaIXtZY4hVVRzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBz[XRiTlnIN3Q{KGOnbHzzJIFnfGW{IEmwJI1qdnNuIFvpQVAvOjZ|IH7N Ml;RNlIxPDd3NU[=
NIH3T3 cells MWHGeY5kfGmxbjDhd5NigQ>? NF\XVHM6OCCvaX7z NY\oVmRYTGm|cHzhZ4Vu\W62IH;mJFIuYzF{NVndbY9ld22nbHH0c45qdiCocn;tJIh2dWGwIF3UNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5icnH0JG5KUDOWMzDj[YxteyCjZoTldkA6OCCvaX7zMEBMcT1yLkOzPUBvVQ>? M2H0N|IzODR5NUW2
CHOK1 NFvE[|FHfW6ldHnvckBie3OjeR?= M2nrS|MhcA>? NHPyO2pFcXOybHHj[Y1mdnRib3[gX|EzPUmfMj3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5icnXjc41jcW6jboSgcYVt[XSxbnnuJJJm[2WydH;yJFEh\XiycnXzd4VlKGmwIFPIU2syKGOnbHzzJIFnfGW{IEOgbJJ{NCCLQ{WwQVAvOjFibl2= NVrzd4ZEOjN2MEOwPFI>
SH-SY5Y cells Ml;tSpVv[3Srb36gZZN{[Xl? NHT1NWcyOCEQvF2= M{O1T|I1KGh? Mnj6UoV2em:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RiSELPNk1qdmS3Y3XkJI95cWSjdHn2[UB{fHKnc4OtZZN{d2OrYYTl[EBl\WG2aDDpckBpfW2jbjDTTE1UYTW\IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHH0JFExKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJJBzcW:{IITvJGgzVzJiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[Xl? MmjFNlY{PjN6Nk[=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
MT1 / MT2 / p-CREB / CREB / BDNF; 

PubMed: 29570621     


Western blot analysis of MT1, MT2, p-CREB, CREB, and BDNF in two cell lines. HT-22 and H19-7 cells were treated with 1 µM melatonin for 2 h;

p-Raf / p-ERK / p-p90RSK / PKAα / p-AKT / p-JNK / p-CaMKII ; 

PubMed: 29570621     


Melatonin regulated the expression of kinases associated with long-term memory processing. HT-22 cells were treated with 1 µM melatonin for 2 h. The expressions of MT1, p-CREB, CREB, p-Raf, Raf, p-ERK, p-p90RSK, BDNF, PKAα, p-AKT, p-JNK, and p-CaMKII were confirmed by Western blot analysis

29570621

Protocol

Solubility (25°C)

In vitro DMSO 47 mg/mL (202.34 mM)
Ethanol 47 mg/mL (202.34 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 232.28
Formula

C13H16N2O2

CAS No. 73-31-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04005222 Not yet recruiting Drug: SELENIUM|Drug: MELATONIN Anterior Segment Ischemia (Diagnosis) Beytepe Murat Erdi Eker State Hospital September 2020 Early Phase 1
NCT04021355 Not yet recruiting Other: Oral sodium supplementation Obesity|Hypertension|Circadian Dysregulation|Salt; Excess University of Alabama at Birmingham January 1 2020 Not Applicable
NCT03934255 Not yet recruiting Other: Constant Routine Protocol Circadian Rhythm|Blood Pressure University of Alabama at Birmingham|American Heart Association October 1 2019 Not Applicable
NCT04078724 Not yet recruiting Dietary Supplement: 5-HTP|Other: Without 5-HTP Sleep|Gut Microbiome National University Singapore October 10 2019 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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MT Receptor Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID