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Catalog No.S1204

10 publications

Melatonin Chemical Structure

Molecular Weight(MW): 232.28

Melatonin is a MT receptor agonist, used as a dietary supplement.

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10mM (1mL in DMSO) USD 90 In stock
USD 97 In stock
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Selleck's Melatonin has been cited by 10 publications

4 Customer Reviews

  • TUNEL staining of treated adipocytes and flow cytometry analysis of positive TUNEL cells (n=3).

    J Pineal Res. 2017, 62(4), doi: 10.1111/jpi.12383. Melatonin purchased from Selleck.

    C: EDU staining of adipocytes treated with MT for 24 h (n=3). D: Flow Cytometry analysis of cell cycle in adipocytes treated with MT for 24 h (n=3).

    J Pineal Res, 2016, doi: 10.1111/jpi.12383.. Melatonin purchased from Selleck.

  • H2O2 activates JNK through the RAC1/MAP2K7 pathway, which is inhibited by melatonin treatment. (A) GCs grown in medium containing 10 μM melatonin for 24 h were rinsed using PBS, and then incubated with 0.2 mM H2O2 for 2 h. For the inhibition of RAC1, the RAC1 specific antagonist NSC 23766 (10 μM) was added 1 h prior to H2O2 exposure. The cell lysates were collected for GST-PAK1-PBD pulldown assay of RAC1 activation (GTP-bound RAC1 levels) and immunoblotting analysis of total RAC1. (B) Western blot analysis of phosphorylated MAP2K7 (p-MAP2K7) and total MAP2K7 in GCs with the indicated treatments as described above. TUBA1A served as the control for loading. (C) The detection of JNK activity in GCs subjected to the treatments as mentioned earlier. Data represent mean ± s.e.; n = 3. **Represents P < 0.01 vs control group. ##Represents P < 0.01 vs H2O2 group. N, not significant, P > 0.05. (D) GCs transfected with scrambled control siRNA (SC siRNA) or Map2k7 siRNA were cultured with 10 μM melatonin for 24 h, washed in PBS, and then grown in medium containing 0.2 mM H2O2 for 2 h. The expression of phosphorylated MAP2K7 (p-MAP2K7) and total MAP2K7 was determined by Western blotting. TUBA1A served as the control for loading. (E) GCs transfected with scrambled control siRNA (SC siRNA) or Map2k7 siRNA for 48 h were cultured with or without 0.2 mM H2O2 for 2 h. Cell lysates were collected for Western blot analysis of RAC1 activation (GTP-RAC1 levels) by GST-PAK1-PBD pulldown and of total RAC1 levels. (F) GCs transfected with scrambled control siRNA (SC siRNA) or Map2k7 siRNA for 24 h were cultured for another 24 h in the presence or absence of 10 μM melatonin before 2 h of H2O2 (0.2 mM) incubation. Cells were then processed for JNK determination. Data represent mean ± s.e.; n = 3. **Represents P < 0.01 vs control group. ##Represents P < 0.01 vs H2O2 group. N, not significant, P > 0.05.

    Reproduction, 2018, 155(3):307-319. Melatonin purchased from Selleck.

    (a-c) Immunohistochemical detection of Nrf2, HO-1 and NQO1 in lumber enlargement of mice from different groups at the peak stage of EAE or on day 28 post immunization.

    J Mol Neurosci, 2018, 64(2):233-241. Melatonin purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Melatonin is a MT receptor agonist, used as a dietary supplement.
melatonin receptor [1]
In vitro

Melatonin interacts with the highly toxic hydroxyl radical with a rate constant equivalent to that of other highly efficient hydroxyl radical scavengers. Melatonin reportedly neutralizes hydrogen peroxide, singlet oxygen, peroxynitrite anion, nitric oxide and hypochlorous acid. [1] Melatonin is believed to scavenge the highly toxic hydroxyl radical, the peroxynitrite anion, and possibly the peroxyl radical. Melatonin reportedly scavenges the superoxide anion radical and it quenches singlet oxygen. Melatonin stimulates mRNA levels for superoxide dismutase and the activities of glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase (all of which are antioxidative enzymes), thereby increasing its antioxidative capacity. [2] Melatonin in cell-free systems has been shown to directly scavenge H2O2, singlet oxygen (1O2) and nitric oxide (NO*), with little or no ability to scavenge the superoxide anion radical (O2*-) in vitro. Melatonin also directly detoxifies the peroxynitrite anion (ONOO-) and/or peroxynitrous acid (ONOOH), or the activated form of this molecule, ONOOH*. Melatonin acts as a direct free radical scavenger with the ability to detoxify both reactive oxygen and reactive nitrogen species. [3] Melatonin inhibits cAMP accumulation in most of the cells examined, but the indole effects on other messengers have been often observed only in one type of the cells or tissue, until now. Melatonin also regulates the transcription factors, namely, phosphorylation of cAMP-responsive element binding protein and expression of c-Fos. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells NEC1R|BHfW6ldHnvckBie3OjeR?= NFfKfppDcW6maX7nJIFn\mmwaYT5JIZweiCqdX3hckBu\WyjdH;ubY4hemWlZYD0c5IhfHmyZTCxRUwh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{ApOi2dMUK1TX1qd2SxbXXsZZRwdmmwIHnzJJV{\WRiYYOgdoFlcW:uaXfhcoQqNCCNaU2wMlIhdk1? NIfyc48yOTB4M{[wNi=>
NIH 3T3 cells NHPRe5lHfW6ldHnvckBie3OjeR?= NEjOUGhDcW6maX7nJIFn\mmwaYT5JJRwf2G{ZIOgdoVkd22kaX7hcpQhcHWvYX6gcYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVGg[ZhxemW|c3XkJIlvKE6LSDCzWFMh[2WubIOgeZNqdmdiMj3bNVIyUV2rb3TvcYVt[XSxbnnuJJJi\GmxbHnnZY5lKGKrbnTpcoch[XO|YYmsJGtqRTBwNk[gcm0> M3\IVFExPzN5N{O4
NIH3T3 cells NFzrRnJHfW6ldHnvckBie3OjeR?= Ml7NRolv\GmwZzDh[oZqdmm2eTDh[4FqdnO2IHj1cYFvKE2WMTDt[YxifG:waX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKE6LSEPUN{Bk\WyuczygT4k:OC52IH7N NEXrN3AyPDZ2M{OzNC=>
CHO cells NX[1Wm1iTnWwY4Tpc44h[XO|YYm= NX6xWJpPUW6qaXLpeIlwdiCxZjD0bIUhOi2dMUK1TX0uKGmxZH;t[YxifG:waX6gZolv\GmwZzD0c{BO\WyjdH;ubY4hemWlZYD0c5IhfHmyZTCxRUBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|LDDLbV05NjByRT2wOUDPxE1? MWe5OFM2QDly
NIH3T3 cells NEPQOJVHfW6ldHnvckBie3OjeR?= NWjWNIZ4SmmwZHnu[{Bi\m[rbnn0fUBi\2GrboP0JIh2dWGwIF3UNkBu\WyjdH;ubY4hemWlZYD0c5Ih\XiycnXzd4VlKGmwIF7JTFNVOyClZXzsd{whU2l;MD6zJI5O NI[5PHIyPDZ2M{OzNC=>
CHO-Galpha16 cells NGLWfpRHfW6ldHnvckBie3OjeR?= MWiyNEBucW6| MmK1Rolv\GmwZzDh[oZqdmm2eTD0c{Bz[XRiTWSxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:tS4FteGijMU[gZ4VtdHNiYYPz[ZN{\WRiYYOgR4EzMyCvb3LpcIl7[XSrb36gZYZ1\XJiMkCgcYlveyCkeTDGUGlRWiCjc4PhfUwhTUN3ME2wMlU3KG6P M1LnO|IyOjN5NkS0
NIH3T3 cells MYnGeY5kfGmxbjDhd5NigQ>? M3:zR|kxKG2rboO= NF7udGJFcXOybHHj[Y1mdnRib3[gNk1cOTJ3SW3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiC{YYSgUmlJO1R|IHPlcIx{KGGodHXyJFkxKG2rboOsJGtqRTBwMk[zJI5O M3z4dVIzODR5NUW2
NIH3T3 cells MnL6SpVv[3Srb36gZZN{[Xl? NUTDRpRjQTBibXnudy=> NInrV|NFcXOybHHj[Y1mdnRib3[gNk1cOTJ3SW3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiC{YYSgUmlJO1R|IHPlcIx{KGGodHXyJFkxKG2rboOsJGtqRTBwM{O5JI5O MknKNlIxPDd3NU[=
CHOK1 NWfvOW5ETnWwY4Tpc44h[XO|YYm= MkDpN{Bp MXLEbZNxdGGlZX3lcpQhd2ZiW{GyOWleOi2rb3TvcYVt[XSxbnnuJIZzd21iaIXtZY4hemWlb33ibY5idnRibXXsZZRwdmmwIILlZ4VxfG:{IEGg[ZhxemW|c3XkJIlvKEOKT1uxJINmdGy|IHHmeIVzKDNiaILzMEBKSzVyPUCuNlEhdk1? MoPkNlM1ODNyOEK=
SH-SY5Y cells NYLEUXRYTnWwY4Tpc44h[XO|YYm= M3nVW|ExKM7:TR?= M3;DSlI1KGh? NWHH[3pVVmW3cn;wdo91\WO2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgTFJQOi2rbnT1Z4VlKG:6aXTheIl3\SC|dILld5Mu[XO|b3PpZZRm\CCmZXH0bEBqdiCqdX3hckBUUC2VWUXZJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIF1KDFyIIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KHC{aX;yJJRwKEh{T{KgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYm= M{\melI3OzZ|OE[2

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
MT1 / MT2 / p-CREB / CREB / BDNF; 

PubMed: 29570621     

Western blot analysis of MT1, MT2, p-CREB, CREB, and BDNF in two cell lines. HT-22 and H19-7 cells were treated with 1 µM melatonin for 2 h;

p-Raf / p-ERK / p-p90RSK / PKAα / p-AKT / p-JNK / p-CaMKII ; 

PubMed: 29570621     

Melatonin regulated the expression of kinases associated with long-term memory processing. HT-22 cells were treated with 1 µM melatonin for 2 h. The expressions of MT1, p-CREB, CREB, p-Raf, Raf, p-ERK, p-p90RSK, BDNF, PKAα, p-AKT, p-JNK, and p-CaMKII were confirmed by Western blot analysis



Solubility (25°C)

In vitro DMSO 47 mg/mL (202.34 mM)
Ethanol 47 mg/mL (202.34 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 232.28


CAS No. 73-31-4
Storage powder
in solvent
Synonyms N/A
Smiles COC1=CC2=C([NH]C=C2CCNC(C)=O)C=C1

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04005222 Not yet recruiting Drug: SELENIUM|Drug: MELATONIN Anterior Segment Ischemia (Diagnosis) Beytepe Murat Erdi Eker State Hospital September 2020 Early Phase 1
NCT04350567 Not yet recruiting Other: Sleep Deprivation Depression; Major Depressive Disorder Stony Brook University September 2020 Not Applicable
NCT04021355 Not yet recruiting Other: Oral sodium supplementation Obesity|Hypertension|Circadian Dysregulation|Salt; Excess University of Alabama at Birmingham July 1 2020 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID