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Technical Data
| Formula | C22H29N7O5.2HCl |
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| Molecular Weight | 544.43 | CAS No. | 58-58-2 | |
| Solubility (25°C)* | In vitro | DMSO | 100 mg/mL (183.67 mM) | |
| Water | 100 mg/mL (183.67 mM) | |||
| Ethanol | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation. |
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| In vitro | The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1] Puromycin can be used for the selection of recombinant cells from noncultured cells. [2] |
| In vivo | Puromycin (aminonucleoside) inhibits protein synthesis leading to proteinuria & glomerulosclerosis/nephrosis.[4] |
Protocol (from reference)
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References
Selleck's Puromycin 2HCl has been cited by 168 publications
| Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis [ Signal Transduct Target Ther, 2024, 9(1):58] | PubMed: 38438346 |
| The dopamine receptor D1 inhibitor, SKF83566, suppresses GBM stemness and invasion through the DRD1-c-Myc-UHRF1 interactions [ J Exp Clin Cancer Res, 2024, 43(1):25] | PubMed: 38246990 |
| Hyperactivation of β-catenin signal in hepatocellular carcinoma recruits myeloid-derived suppressor cells through PF4-CXCR3 axis [ Cancer Lett, 2024, 586:216690] | PubMed: 38307410 |
| DHCR7 promotes lymph node metastasis in cervical cancer through cholesterol reprogramming-mediated activation of the KANK4/PI3K/AKT axis and VEGF-C secretion [ Cancer Lett, 2024, 584:216609] | PubMed: 38211648 |
| SHMT2 promotes papillary thyroid cancer metastasis through epigenetic activation of AKT signaling [ Cell Death Dis, 2024, 15(1):87] | PubMed: 38272883 |
| MDM2 provides TOP2 poison resistance by promoting proteolysis of TOP2βcc in a p53-independent manner [ Cell Death Dis, 2024, 15(1):83] | PubMed: 38263255 |
| METTL3 facilitates renal cell carcinoma progression by PLOD2 m6A-methylation under prolonged hypoxia [ Cell Death Dis, 2024, 15(1):62] | PubMed: 38233403 |
| Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers [ Cell Death Dis, 2024, 15(2):149] | PubMed: 38365764 |
| Dual roles of CCDC102A in governing centrosome duplication and cohesion [ Cell Rep, 2024, 43(2):113696] | PubMed: 38280197 |
| EP300-ZNF384 transactivates IL3RA to promote the progression of B-cell acute lymphoblastic leukemia [ Cell Commun Signal, 2024, 22(1):211] | PubMed: 38566191 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.