Staurosporine

For research use only.

Catalog No.S1421 Synonyms: CGP 41251

73 publications

Staurosporine Chemical Structure

Molecular Weight(MW): 466.53

Staurosporine is a potent PKC inhibitor for PKCα, PKCγ and PKCη with IC50 of 2 nM, 5 nM and 4 nM, less potent to PKCδ (20 nM), PKCε (73 nM) and little active to PKCζ (1086 nM) in cell-free assays. Also shows inhibitory activities on other kinases, such as PKA, PKG, S6K, CaMKII, etc. Phase 3.

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Selleck's Staurosporine has been cited by 73 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Staurosporine is a potent PKC inhibitor for PKCα, PKCγ and PKCη with IC50 of 2 nM, 5 nM and 4 nM, less potent to PKCδ (20 nM), PKCε (73 nM) and little active to PKCζ (1086 nM) in cell-free assays. Also shows inhibitory activities on other kinases, such as PKA, PKG, S6K, CaMKII, etc. Phase 3.
Targets
PKCα [1]
(Cell-free assay)
c-Fgr [2]
(Cell-free assay)
phosphorylase kinase [2]
(Cell-free assay)
PKCη [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
2 nM 2 nM 3 nM 4 nM 5 nM
In vitro

Staurosporine, a microbial alkaloid, significantly inhibits protein kinase C from rat brain with IC50 of 2.7 nM. Staurosporine displays strong inhibitory effect against HeLa S3 cells with IC50 of 4 nM. [1] Staurosporine also inhibits a variety of other protein kinases, including PKA, PKG, phosphorylase kinase, S6 kinase, Myosin light chain kinase (MLCK), CAM PKII, cdc2, v-Src, Lyn, c-Fgr, and Syk with IC50 of 15 nM, 18 nM, 3 nM, 5 nM, 21 nM, 20 nM, 9 nM, 6 nM, 20 nM, 2 nM, and 16 nM, respectively. [2] Staurosporine (1 μM) induces >90% apoptosis in PC12 cells. Consistently, Staurosporine treatment induces a rapid and prolonged elevation of intracellular free calcium levels [Ca2+]i, accumulation of mitochondrial reactive oxygen species (ROS), and subsequent mitochondrial dysfunction. [3] The apoptosis of MCF7 cells induced by Staurosporine can be enhanced by the expression of functional caspase-3 via caspase-8 activation and Bid cleavage. [4] Staurosporine treatment at 1 μM only partially inhibits IL-3-stimulated Bcl2 phosphorylation but completely blocks PKC-mediated Bcl2 phosphorylation. [5] Staurosporine induces apoptosis of human foreskin fibroblasts AG-1518, depending on the lysosomal cathepsins D mediated cytochrome c release and caspase activation. [6] In addition to activating the classical mitochondrial apoptosis pathway, Staurosporine triggers a novel intrinsic apoptosis pathway, relying on the activation of caspase-9 in the absence of Apaf-1. [7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HeLa cells MULDfZRwfG:6aXRCpIF{e2G7 MVe0PEBp NVG5SWhFS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVRmNTB4IN88UU4> NGfJcFYzOTN6OEG5NS=>
human colon cancer cell line (LoVo cells) M2HvU3Bzd2yrZnXyZZRqd25iYYPzZZk> MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKGOxbH;uJINidmOncjDj[YxtKGyrbnWgLGxwXm9iY3XscJMqKHW|aX7nJG1VXCCjc4PhfUwhUUN3ME2wMlAxOSEQvF2u NYHjcm8xOTF3OUG1NFU>
human LoVo cells MXXQdo9tcW[ncnH0bY9vKGG|c3H5 M{ftVFQ5KHSxIEeyJIg> MnfRRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOb2\vJINmdGy|IHHmeIVzKDR6IITvJFczKGi{czDifUBOXFRiYYPzZZk> MV[yNlE5Ojl{OR?=
P19 cells NWXXS2MxTnWwY4Tpc44h[XO|YYm= NE\LcnRKdmirYnn0bY9vKG:oIGDsZZRmdGW2LXTldol3\WRiZ4Lve5RpKG[jY4TvdkBz\WOncITvdkBqdiCSMUmgZ4VtdHNuIFnDOVA:OC5yMEKg{txONg>? MnyzNVU4PzF2MUm=
human BJ cells M1XxTmN6fG:2b4jpZ:Kh[XO|YYm= M{XHRVczKGh? MXHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCTkBk\WyuczDh[pRmeiB5MjDodpMh[nliQ3HsZ4VqdiCDTTDhd5NigSxiSVO1NF0xNjByMjFOwG0v NF3rSo4zOjl{MUC4NS=>
human HT-29 cells M2fDTWZ2dmO2aX;uJIF{e2G7 NY\acYFCOiCq NEjPNpRG\m[nY4Sgc44hdWm2b3Poc45lemmjbDDt[Y1jemGwZTDwc5RmdnSrYXygbY4hcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgNkBpenNidYPpcochUkNvMTDzeIFqdmmwZzDifUBndHWxcnXzZ4Vv[2ViYYPzZZk> NH[4TFkzOTR{OEO3OS=>
human A549 cells MV;DfZRwfG:6aXRCpIF{e2G7 NEnNdXE4OiCq NH20d3BEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFczKGi{czDifUB{fWyob4Loc4RidWmwZTDCJI1mfGixZB?= MkG0NVg1QDR5N{W=
human HT-29 cells MoW5SpVv[3Srb36gZZN{[Xl? NYnQS3g4UW6qaXLpeIlwdiCxZjDtbZRw[2ixbnTybYFtKG2nbXLyZY5mKHCxdHXueIlidCCrbjDoeY1idiCKVD2yPUBk\WyuczD1d4lv\yCMQ{Gg[JlmKHO2YXnubY5oKGK7IH\seY9z\XOlZX7j[UBxdGG2ZTDy[YFl\XJiYYPzZZktKEmFNUC9Nk42KG6P NHTLVlMzOTVzM{K5Ny=>
human HT-29 cells Mn3nSpVv[3Srb36gZZN{[Xl? NIL1WmUzKGh? NGjqdZBKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFjUMVI6KGOnbHzzJIF{e2W|c3XkJJJm\HWldHnvckBw\iCvaYTvZ4hwdmS{aXHsJI1mdWK{YX7lJJBwfGWwdHnhcEBi\nSncjCyJIhzeyCkeTD1d4lv\yCMQ{Ggd5RicW6rbnegZpkh\my3b4Lld4NmdmOnIHPlcIwu[mG|ZXSgZZN{[XluIFXDOVA:Oi54IH7NMi=> NEHUdHkzOTl5M{GwNS=>
Sf9 cells MWrGeY5kfGmxbjDhd5NigQ>? MYHJcohq[mm2aX;uJI9nKGi3bXHuJHN6cyCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzMEBKSzVyPUOgcm0v NW\ycmJ6OTh6MkO3PFQ>
human HUVEC M1;sTnBzd2yrZnXyZZRqd25iYYPzZZk> MojTOFghfG9iN{KgbC=> MYDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiXVlXDJIFnfGW{IES4JJRwKDd{IHjyd{BjgSCPVGSgZZN{[Xl? M1;GO|IzOTh{OUK5
P19 cells NUnjZ5YyTnWwY4Tpc44h[XO|YYm= M3zSNmlvcGmkaYTpc44hd2ZiUILveIVqdiCNaX7hd4UhSSCrbjDQNVkh[2WubIOsJGlEPTB;NDDuUU4> M4fV[|E2PzdzNEG5
Sf9 cells MUnGeY5kfGmxbjDhd5NigQ>? M1zPPWlvcGmkaYTpc44hd2ZiaIXtZY4hTnmwIHX4dJJme3OnZDDpckBU\jliY3XscJMh[W[2ZYKgNUBucW5iYomgSWxKW0FiaX6gdJJme2WwY3Wgc4YhOSC3bX;sM2whSVSS Mkj6NVc{OTV6NUO=
Sf21 cells M1;n[2Z2dmO2aX;uJIF{e2G7 MVjJcohq[mm2aX;uJI9nKEqDS{Og[ZhxemW|c3XkJIlvKFOoMkGgZ4VtdHNuIFnDOVA:PiCwTT6= M3jFNFE4ODh6MEW5
human colon carcinoma cell line HCT116 M{S2eGZ2dmO2aX;uJIF{e2G7 MUfDc45k\W62cnH0bY9vKHKncYXpdoVlKG[xcjDndo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDjc4xwdiClYYLjbY5wdWFiY3XscEBtcW6nIFjDWFEyPixiSVO1NF03KG6PLh?= MWWxOVU{PzN2NR?=
human ST486 cells NEGxOlVRem:uaX\ldoF1cW:wIHHzd4F6 NXTzPGVoPDhidH:gO|IhcA>? MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOWNEi2JINmdGy|IHHmeIVzKDR6IITvJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9O{BvVS5? M{j6elIzOTh{OUK5
human MDA-MB-231 cells MVLDfZRwfG:6aXRCpIF{e2G7 M1rlZVczKGh? M2\sdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJibXX0bI9lNCCJSUWwQVcvOSCwTT6= NIfRfJMyQDR6NEe3OS=>
P19 cells M{\Q[mZ2dmO2aX;uJIF{e2G7 Ml7PTY5pcWKrdHnvckBw\iCFeXPsbY4u\GWyZX7k[Y51KGurbnHz[UAyKGmwIGCxPUBk\WyuczygTWM2OD16IH7NMi=> NX;oS4ZEOTV5N{G0NVk>
human DLD1 cells MmK2VJJwdGmoZYLheIlwdiCjc4PhfS=> Ml35OFguPzJiaB?= NYq5OFFnSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDEUGQyKGOnbHzzJIFnfGW{IES4JJRwKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QSCwTT6= NUDRO|h6OjJzOEK5Nlk>
insect cells MXHGeY5kfGmxbjDhd5NigQ>? Mlu5TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCSaX2xJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgZpkhUFSURjygTWM2OD1zMDDuUU4> MoXjNVkyPzlyN{[=
V79 MZ cells M1v5NmZ2dmO2aX;uJIF{e2G7 Ml\NTY5pcWKrdHnvckBw\iCqdX3hckBidGSxc4Tldo9v\SC|eX70bIF{\SCneIDy[ZN{\WRiaX6gWlc6KE2cIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[Wymb4P0[ZJwdmVic4nueIhme2m|LDDJR|UxRTFzIH7NMi=> NUL1ZlQ4OjR2MkK1NVk>
P19 cells MlPzSpVv[3Srb36gZZN{[Xl? M{n0bWlvcGmkaYTpc44hd2ZiVnHzZ5Vt[XJiZX7kc5Rp\WyrYXyg[5Jwf3SqIH\hZ5RweiC{ZXPldJRweiCrbjDQNVkh[2WubIOsJGlEPTB;MUSgcm0v M1jyd|E2PzdzNEG5
Sf9 cells MVfGeY5kfGmxbjDhd5NigQ>? NYDhenY6OjBibXnudy=> NIrPSJhKdmirYnn0bY9vKG:oIHj1cYFvKE[7bjDlfJBz\XO|ZXSgbY4hW2Z7IHPlcIx{KGGodHXyJFIxKG2rboOgZpkhTUyLU1GgbY4heHKnc3XuZ4Uhd2ZiMTD1cY9tN0xiQWTQMEBKSzVyPUG1JI5ONg>? MmD4NVc{OTV6NUO=
human PBMC NH7DU4xHfW6ldHnvckBie3OjeR?= MkXWNlQhcA>? NGnvfZNUfXCycnXzd4lwdiCxZjDJUFIheHKxZIXjeIlwdiCrbjDoeY1idiCSQl3DJIFnfGW{IEK0JIhzeyCkeTDFUGlUSSxiSVO1NF0yPiCwTT6= NIfRUIwyQDV6NUC0Oi=>
human A549 cells MkPqR5l1d3SxeHnjxsBie3OjeR?= MlLFOFghcA>? MnLuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5JEwhUUN3ME2yNEBvVS5? MoTKNlU5OjV7M{S=
human CEM cells NH7BfnREgXSxdH;4bYPDqGG|c3H5 M4i3d|czKGh? MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDSW0h[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPhcINmcW5iQV2gZZN{[XluIFnDOVA:OjNibl2u NE\kNVEzOjl{MUC4NS=>
human HeLa cells NVrufZJOS3m2b4TvfIlkyqCjc4PhfS=> NV23dXRoPDhiaB?= NV7JOFMxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVI2KG6PLh?= NYXGfm9{OjV6MkW5N|Q>
human PC3 cells M4rtWGN6fG:2b4jpZ:Kh[XO|YYm= NX;MTYVRPDhiaB?= NFW5dJJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUAtKEmFNUC9N|Ehdk1w NITxWoQzPTh{NUmzOC=>
human SF268 cells Mmq2R5l1d3SxeHnjxsBie3OjeR?= MnK4OFghcA>? NVjlZYwxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0Z{NkigZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF01PCCwTT6= MnmwNlE2OTN{OUS=
human MCF7 cells MVXDfZRwfG:6aXRCpIF{e2G7 NF\DbYs1QCCq NF7L[YJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OVAhdk1w M4jLU|IyOzh6MUmx
HEK293 cells M2TsZ2N6fG:2b4jpZ:Kh[XO|YYm= MVu3NkBp M4\hcWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{R3zvJIF{e2G7LDDJR|UxRTV4IH7NMi=> NWW1VldwOjR5NkOyOlI>
HUE cells NFjQTYlHfW6ldHnvckBie3OjeR?= NIXSb5c6OCCvaX7z NInvb4hKdmirYnn0bY9vKG:oIG\FS2ZTOiCrbjDIWWUh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDWSWdHNWmwZIXj[YQh[XW2b4Doc5NxcG:{eXzheIlwdiC2cnXheIVlKG[xcjC5NEBucW6|IHLl[o9z\SCYRVfGJINp[WyuZX7n[UBjgSCHTFnTRUwhUUN3ME23NEBvVS5? M2rROFIxOTdyMU[z
human A431 cells NIPTWZlEgXSxdH;4bYPDqGG|c3H5 MUeyOEAhcA>? M1u3bmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE1OzFiY3XscJMh[W[2ZYKgNlQhcHK|IIXzbY5oKEGwbnX4bY4hXmWISWTDM5Bzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnegZpkhVVSWIHHzd4F6NCCLQ{WwQVcxKG6PLh?= MmrRNlI2PDFyNUG=
human Jurkat cells MY\Qdo9tcW[ncnH0bY9vKGG|c3H5 MljVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDKRWs{KGW6cILld5NqdmdiSVyyMZN1cW23bHH0[YQhcHWvYX6gTpVzc2G2IHPlcIx{NCCLQ{WwQVcyKG6PLh?= MkDYNVk1Ojd{MEO=
HEK293 cells MX\GeY5kfGmxbjDhd5NigQ>? NFWyUoJKdmirYnn0bY9vKG:oIFnMMVghemWuZXHz[UBjgSCKRVuyPVMh[2WubIOg[ZhxemW|c3nu[{BRU0NvYnX0ZVItKEmFNUC9O|chdk1w M4TDW|E2PzdzNEG5
human KE-97 cells NWnkcWwyS3m2b4TvfIlkyqCjc4PhfS=> MXO3NkBp NFXzT5ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMTS17NzDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeJJmNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF|IN88UU4> M3TueVI1OzJ6Mkiz
human CHOK1 cells M2TqbGN6fG:2b4jpZ:Kh[XO|YYm= M2O2NlQ5KGh? M3LWWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNJV0tzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZnwxIxiSVO1NF0xNjF|IN88UU4> Mk\FNlE2OTN{OUS=
mouse NIH/3T3 cells NF7XR|BEgXSxdH;4bYPDqGG|c3H5 MUO5OkBp NXfu[nppS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhVkmKL{PUN{Bk\WyuczDh[pRmeiB7NjDodpMh[nliU2LCJIF{e2G7LDDJR|UxRTBwMjFOwG0v NYfwWlZHOjR|NkG1NlE>
human A2780 cells MXLDfZRwfG:6aXRCpIF{e2G7 M4XPb|k3KGh? MnHWR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlIh|ryPLh?= NFTjVlUzPDN4MUWyNS=>
human 8505C cells MnnjR5l1d3SxeHnjxsBie3OjeR?= MW[5OkBp MkHBR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gPFUxPUNiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlIh|ryPLh?= M{S0S|I1OzZzNUKx
human 518A2 cells NEnqNndEgXSxdH;4bYPDqGG|c3H5 MmO1PVYhcA>? NIfGbphEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckA2OTiDMjDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F697zOIFnDOVA:OC5{IN88UU4> MnjNNlQ{PjF3MkG=
human HuH7 cells MXPDfZRwfG:6aXRCpIF{e2G7 NEnyOYs4OiCq MmroR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVJPyClZXzsd{Bi\nSncjC3NkBpenNiYomgR4VtdFSrdILlMWdtdyCudX3pcoV{[2WwdDDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlI{KM7:TT6= NYHPPI1SOjR|MkiyPFM>
FL5.12-Akt1 cells MkLYVJJwdGmoZYLheIlwdiCjc4PhfS=> NHro[4dCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGZNPS5zMj3Bb5QyKGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjJ7IN88UU4> MVWxOlQxOzZ{Nh?=
human MiaPaCa-2 cells NXPBSVBZWHKxbHnm[ZJifGmxbjDhd5NigQ>? MmnhRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPaXHQZWNiNTJiY3XscJMtKEmFNUC9NE4{PyEQvF2u M2izTlE3PDF|N{iw
human BGC823 cells NH3IcoxEgXSxdH;4bYPDqGG|c3H5 MXG3NkBp MlGwR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmdEQDJ|IHPlcIx{KGGodHXyJFczKGi{czDifUBE\WyuVHn0doUuT2yxIHz1cYlv\XOlZX70JINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzhizszNMi=> Mmn0NlQ{Ojh{OEO=
human MCF7 cells Ml32R5l1d3SxeHnjxsBie3OjeR?= MWK5OkBp M4ftdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlQh|ryPLh?= NFzFRnkzPDN4MUWyNS=>
human A549 cells MlS4R5l1d3SxeHnjxsBie3OjeR?= NEnFTpE6PiCq Ml;vR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUCuOkDPxE1w MXiyOFM3OTV{MR?=
HEK293 cells MX\DfZRwfG:6aXRCpIF{e2G7 NUHuPFExS3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlk{KGOnbHzzMEBGSzVyPUKg{txONg>? MlL5NlU{OTZ|MUe=
human Raji cells  Mn7TR5l1d3SxeHnjxsBie3OjeR?= NXXXS2szS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWmGsaTDj[YxteyxiRVO1NF0zKM7:TT6= MVmyOVMyPjNzNx?=
human HepG2 cells NHnaT5pEgXSxdH;4bYPDqGG|c3H5 M17x[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{NCCHQ{WwQVIh|ryPLh?= MoCzNlU{OTZ|MUe=
human BJ cells MVrDfZRwfG:6aXRCpIF{e2G7 M1\MPWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJLKGOnbHzzMEBGSzVyPUKg{txONg>? Ml;VNlU{OTZ|MUe=
human U937 cells Ml3rR5l1d3SxeHnjxsBie3OjeR?= MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDVPVM4KGOnbHzzMEBKSzVyPUKg{txONg>? NUC0WYRQOTdyOEiwOlc>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Akt / Akt / PARP /Cleaved PARP; 

PubMed: 24174874     


Western blot analysis for Akt, P-Akt, and cleaved poly(ADP-ribose) polymerase. U87 cells were incubated with 200 nM, 10 nM, and 0.1 nM of staurosporine encapsulated in liposomes and staurosporine for 32 hours.

FAK / RIP; 

PubMed: 15121855     


Western blot (WB) analysis of BT-20 and BT-474 lysates treated with staurosporine (200 nM). Control cells were treated with dimethyl sulfoxide for 18 h.

Ambra1; 

PubMed: 24587252     


SW620 cells treated with various doses of staurosporine for 6 h. Ambra1 levels were measured by Western blot. 

24174874 15121855 24587252
Growth inhibition assay
Cell viability; 

PubMed: 25215174     


Staurosporine reduces cerebellar astrocytes viability. (a) Cerebellar astrocytes were treated with staurosporine 0.1 μM, 0.25 μM, and 0.5 μM for 24 h and the cell viability was measured by MTT transformation. (b) Cerebellar astrocytes were treated with staurosporine 0.5 μM for 6, 12, and 24 h and the cell viability was measured by MTT transformation. Data are presented as mean ± SEM of four independent experiments. ∗ is significantly different from control (P < 0.05).

25215174
Immunofluorescence
Tubulin / Actin; 

PubMed: 25215174     


Morphological changes of astrocytes induced by St are evidenced by the rearrangement of cytoskeletal proteins. Astrocytes were treated with St (0.5 μM) for 12 hours and then were labelled with rhodamine-phalloidin or immunostained for tubulin. Representative images of phase contrast, rhodamine-phalloidin, and tubulin are shown in control and St treated astrocytes. Scale bar represents 50 μm.

Phalloidin / Type II collagen; 

PubMed: 22684244     


Cell were cultured in the absence (a-f) or presence of staurosporine (STSN, 5 × 10-9M) or cytochalasin D (CD, 1 µg/ml) for 1 (upper panel) or 2 (lower panel) days at low density and stained for F-actin (Phalloidin) and type II collagen (Type II). STSN: Staurosporine.

Pyk2; 

PubMed: 19880522     


Pyk2 translocates to the nucleus upon staurosporine addition to ID8 cells. Confocal immunofluorescent analysis of endogenous Pyk2 upon DMSO (control) or 1 μm staurosporine addition for 2 h. The scale bar is 10 μm. 

Annexin; 

PubMed: 15140398     


Induction of apoptosis with staurosporine resulting in activation of the ICE-NIRF probe. Gli36 cells were treated with 50 µM staurosporine for 24 hours (A-C) or with the same percentage of DMSO (0.01%) to which experimental wells were exposed (D-488nm laser, E-633nm laser and F-bright field). To examine the role of caspase-1 in staurosporine-induced apoptosis and probe activation, cells were coincubated in caspase-1 inhibitor (10 µM) and staurosporine (G-I). Staurosporine induces apoptosis, indicated by the positive annexin staining viewed with the 488-nm laser (A), which colocalized with activated probe viewed with the 633-nm laser (B). Coincubation of the caspase-1 inhibitor with staurosporine did not completely block apoptosis, indicated by the relatively higher number of apoptotic cells stained with annexin (G), as those that activated the probe (H). Magnification, x 40; scale bar, 50 µM.

cleaved-caspase 3; 

PubMed: 19840952     


Representative images (×1000 magnification) showing vehicle treated (C) and staurosporine treated (D) cells stained with anti-cleaved caspase 3 antibody and DAPI.

FAK 4.47; 

PubMed: 15121855     


BT-20 or BT-474 cell culture were plated on six-well plates and were treated with staurosporine (200 nM) after 24 h. After 6 h of treatment, cells were immunostained with anti-FAK 4.47 antibody (white arrowheads mark some focal adhesions).

25215174 22684244 19880522 15140398 19840952 15121855
In vivo In the gerbil and rat ischemia models, Staurosporine pretreatment (0.1-10 ng) before ischemia prevents neuronal damage in a dose-dependent manner, suggesting the involvement of PKC in CAl pyramidal cell death after ischemia. [8]

Protocol

Kinase Assay:

[1]

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Enzyme assay and binding assay:

Protein kinase C is assayed in a reaction mixture (0.25 mL) containing 5 μmol of Tris/HCl, pH 7.5, 2.5 μmol of magnesium acetate, 50 μg of histone II S, 20 μg of phosphatidylserine, 0.88 μg of diolein, 125 nmol of CaCl2, 1.25 nmol of [γ-32]ATP (5-10 × 104 cpm/nmol) and 5 μg of partially purified enzyme. The binding of [3H]PDBu to protein kinase C is determined: Reaction mixture (200 μL contained 4 μmo1 of Tris/malate, pH 6.8, 20 μmol of KCl, 30 nmol of CaC12, 20 μg of phosphatidylserine, 5 μg of partially purified protein kinase C, 0.5% (final concentration) of DMSO,10 pmol of [3H]PDBu (l-3 × 104 cpm/pmol) and 10 μL of various amounts of Staurosporine.
Cell Research:

[3]

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  • Cell lines: PC12
  • Concentrations: Dissolved in DMSO, final concentration 1 μM
  • Incubation Time: ~32 hours
  • Method:

    Cells are exposed to Staurosporine for ~32 hours. Cells are fixed in 4% paraformaldehyde and stained with the DNA-binding dye Hoechst 33342. Cells are visualized under epifluorescence illumination, and the percentage of apoptotic cells (cells with condensed and fragmented DNA) is determined.


    (Only for Reference)
Animal Research:

[8]

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  • Animal Models: Male Mongolian gerbils or male Wistar rats subjected to transient ischemia
  • Dosages: ~10 ng
  • Administration: Stereotaxically administered into the bilateral CAl subfield of the hippocampus
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 4 mg/mL (8.57 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 466.53
Formula

C28H26N4O3

CAS No. 62996-74-1
Storage powder
in solvent
Synonyms CGP 41251

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID