Tamoxifen

Catalog No.S1238 Synonyms: ICI 46474

Tamoxifen Chemical Structure

Molecular Weight(MW): 371.51

Tamoxifen is an antagonist of the estrogen receptor in breast tissue.

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Cited by 4 Publications

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  • E, Analysis of BrCSC viability upon treatment with tamoxifen (tam) and fulvestrant (fulv) at the indicated doses. The experiments were performed in triplicates. ** P<0.01.

    Oncotarget, 2015, 6(4):2315-30.. Tamoxifen purchased from Selleck.

Purity & Quality Control

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description Tamoxifen is an antagonist of the estrogen receptor in breast tissue.
Targets
estrogen receptor [1]
In vitro

TAM treatment inhibits significantly MCF7 cell proliferation. Low doses of TAM are able to induce structural chromosomal aberrations (deletions, isochromosomes, translocations, and dicentric chromosomes) in both ER+ and ER- breast cancer cells. This genotoxic effect is higher in those cell lines with HER2 gene amplification[2]. Whereas TAM at lower concentrations (0.1-1 μM) induces a cell-cycle arrest, pharmacological concentrations (above 5 μM) of TAM have been found to induce apoptosis of breast cancer cells. 5 μM TAM rapidly induced sustained activation of ERK1/2 in ER-positive breast cancer cell lines (MCF-7 and T47D)[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7 cells NHzwNphHfW6ldHnvckBie3OjeR?= M{DPZWVn\mWldHn2[UBkd26lZX70doF1cW:wIHHnZYlve3RiTVPGMVch[nKnYYP0JJR2dW:{IHPlcIx{KHW|aX7nJG1ETi15IHHzd4F6NCCHQ{WwQVAvODFizszN MmPWNVI5OjV7M{W=
MCF7 cells NVrRWFdVS3m2b4TvfIlkcXS7IHHzd4F6 NEXCPYI1QCCq NEHib5hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUC9NE4xOjdizszN MkH4NlI1PzJyNEW=
Ishikawa cells NGjpcmFHfW6ldHnvckBie3OjeR?= MYDDc45k\W62cnH0bY9vKG:oIHPvcZBwfW6mIILldZVqemWmIITvJIlv\HWlZTC1NEAmKG:oIITo[UBu[XirbYXtJJN1cW23bHH0bY9vKG:oIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JIlvKEm|aHnrZZdiKGOnbHzzMEBGSzVyPUCuNFM{KM7:TR?= NHTLSYI5OjBzNUi3
HEK293T cells NIr2cWpHfW6ldHnvckBie3OjeR?= M4D3d2FvfGGpb37pd5Qh[WO2aY\peJkh[XRiR3HsOEBFSkRvZoXz[YQhcHWvYX6gSXJidHCqYTDMRmQh\XiycnXzd4VlKGmwIFjFT|I6O1RiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBme3S{YXTpc4wucW6mdXPl[EB1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBi\nSncjCyNEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwQVAvODN7IN88US=> Ml7qNlM4QDZ2NUK=
MDA-MB-435 cells NILBNFJHfW6ldHnvckBie3OjeR?= NGHnbIpKdmirYnn0bY9vKG:oIF3ERU1OSi12M{WgZpJm[XO2IHPhcoNmeiClZXzsJJBzd2yrZnXyZZRqd25uIFnDOVA:OC5yNTFOwG0> MX6xOFk4OTh7OR?=
MDA-MB-231 cells MoW0SpVv[3Srb36gZZN{[Xl? MnzRTY5pcWKrdHnvckBw\iCnc4Tyc4dmdiC{ZXPldJRweiCwZXfheIl3\SCPRFGtUWIuOjNzIHLy[YF{fCClYX7j[ZIh[2WubDDwdo9tcW[ncnH0bY9vNCCLQ{WwQVAvODhizszN MWKxOFk4OTh7OR?=
T47D cells Mn3YSpVv[3Srb36gZZN{[Xl? M2m4XWFvfGmnc4Tyc4dmdmmlIHHjeIl3cXS7IHnuJIh2dWGwIGS0O2Qh[2WubIOg[ZhxemW|c3nu[{Bme3S{b3flckBz\WOncITvdkBie3Onc4Pl[EBieyCnc4Tyc4dmdi2mZYDlcoRmdnRidILhcpNkemmydHnvckBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTB;MD6xJO69VQ>? NYrUeGwyOTlzM{GyOFg>
Ishikawa cells MX;GeY5kfGmxbjDhd5NigQ>? MkOzO|IhcA>? NETYZ5FCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDJd4hqc2G5YTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGW|dILv[4VvNWmwZIXj[YQh[WytYXzpcoUheGixc4DoZZRie2ViYXP0bZZqfHliYX\0[ZIhPzJiaILzMEBKSzVyPUCuNVch|ryP M3X0N|E5QDN3MUe2
MCF-7-2a cells Mk[1SpVv[3Srb36gZZN{[Xl? MXfBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDNR2YuPy1{YTDj[YxteyCjczDjc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIILl[JVk\SCnc4TyZYRqd2xiZX\m[YN1KGK7IEWwKUwhUUN3ME2wMlUh|ryP NYLCSnB7OTJ2M{GwOlM>
EL4 cell MlPUVJJwdGmoZYLheIlwdiCjc4PhfS=> NXzOV4FoUW6qaXLpeIlwdiCxZjDbN2hefGi7bXnkbY5mKGmwY3;ydI9z[XSrb36gbY51dyCHTESgZ4VtdCCycn;sbYZmemG2aX;uMEBq[zVyPUCuOVM2KM7:TR?= MknZNVU4OzZ|MB?=
HeLa cells M4PQZWZ2dmO2aX;uJIF{e2G7 M3fUcWlvcGmkaYTpc44hd2ZiMUetZoV1[S2nc4TyZYRqd2xibXXkbYF1\WRibIXjbYZmemG|ZTD0doFve2O{aYD0bY9vKGmwIFjlUIEh[2WubIOg[ZhxemW|c3nu[{BpfW2jbjDld5Rzd2enbjDy[YNmeHSxcjDhcJBp[TtiRWLFJIF{e2G7LDDJR|UxRTBwNkKyJO69VQ>? M2fMXVE2PjV6OEWx
MDA-MB-231 cells NIrkWYpRem:uaX\ldoF1cW:wIHHzd4F6 MUTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxRTBwNk[g{txO NFHQbWIzODR3MUO4NC=>
T47D cells NUe2fY14T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEXodG84OiCq MWfHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDld5Rzd2enbj3k[ZBmdmSnboSgWFQ4TCClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:OSEQvF2= M{eyV|E6OTNzMkS4
yeast AH109 cells NVXGeXpqTnWwY4Tpc44h[XO|YYm= MU[yOEBp NHzsPZZCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFXSZoV1[SC{ZXPldJRweiCOQlSg[ZhxemW|c3XkJIlvKHmnYYP0JGFJOTB7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcW62ZYLhZ5Rqd25id3n0bEBUWkNzIHHmeIVzKDJ2IHjyd{BjgSCjbIDoZU1o[WyjY4Tvd4ll[XOnIHHzd4F6NCCLQ{WwQVEvPjZizszN NXnMWlgyOjJ4NEeyNVc>
GBM2 cells NEj1ZYZRem:uaX\ldoF1cW:wIHHzd4F6 M1rt[VczKGh? Ml7rRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCJQl2yJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBqdmO3YnH0[YQh\m:{IEeyJIhzeyCkeTDXV3QuOSCvZYToc4QtKEmFNUC9N{42KM7:TR?= MmnXNlY{PTV3M{K=
NCI-H460 cells Mn;RSpVv[3Srb36gZZN{[Xl? NGPkeWozPCCq NFrRZ5dCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUmNKNUh2NkCgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF01NjR6IN88US=> MWmxPVc{OzB6NR?=
GBM3 cells M1nwcXBzd2yrZnXyZZRqd25iYYPzZZk> MXm3NkBp M4fOU2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNN{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVQvPyEQvF2= MWSyOlM2PTV|Mh?=
DLD1 cells NULKTGpRS3m2b4TvfIlkcXS7IHHzd4F6 NWr3Umx1QTZiaB?= MlSyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSGxFOSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUSuO|gh|ryP NVjqTG9KOjJ5NEmzPVI>
ScN2a-cl3 cells M13Ic2N6fG:2b4jpZ4l1gSCjc4PhfS=> MU[1JIRigXN? NX24SmVLS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4Uh\Gm4aXTpcochW2OQMnGtZ4w{KGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IEWg[IF6eyCkeTDjZYxk\WmwLVHNJJN1[WmwaX7nMYJie2WmIH\seY9z\XOlZX7j[UBie3OjeTygTWM2OD12Lki5JO69VQ>? NGf5XIwzPDF6M{W4PS=>
K562 cells M2rSZ3Bzd2yrZnXyZZRqd25iYYPzZZk> NWmy[lBwPzJiaB?= MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEt3NkKgZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUWuNkDPxE1? MX6yOVQzODF5NR?=
MDA-MB-435S cells MmjpR5l1d3SxeHnjbZR6KGG|c3H5 NF7a[YZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtOFM2WyClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBjgSCPVGSgZZN{[XluIFnDOVA:PS53IN88US=> MUOyOlA4QTB7MB?=
DU145 cells MlzIVJJwdGmoZYLheIlwdiCjc4PhfS=> Ml\KOFghcA>? NWDkNmV[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUWuOUDPxE1? NX7iOHJqOjN{OEewOVc>
HepG2 cells NHv6eoRRem:uaX\ldoF1cW:wIHHzd4F6 MknHOFghcA>? NGXOOIhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Nj6zJO69VQ>? NX\nZ5loOjN{OEewOVc>
SKOV3 cells M17KN2Z2dmO2aX;uJIF{e2G7 MXuyOEBp MoXhRY51cWOjbnPldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLU3Y{KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;Nj60JO69VQ>? MXyxPVc{OzB6NR?=
PC3 cells NFvxTY9Rem:uaX\ldoF1cW:wIHHzd4F6 M2H1bVI1KGh? NWXsZVhQSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD15IN88US=> NYHrZoxMOjR6OECyN|A>
NIH/3T3 cells M1jMdWN6fG:2b4jpZ4l1gSCjc4PhfS=> NYnJSHZlQTZiaB?= Mn3LR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUmlJNzOWMzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvOjZizszN M{\rU|IzPzR7M{my
GBM1 cells MornVJJwdGmoZYLheIlwdiCjc4PhfS=> M4D2XlczKGh? NGPQc2xCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFfCUVEh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgO|IhcHK|IHL5JHdUXC1zIH3leIhw\CxiSVO1NF04NjVizszN MVmyOlM2PTV|Mh?=
518A2 cells NVTjXppqS3m2b4TvfIlkcXS7IHHzd4F6 NWe3TFhDQTZiaB?= MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC1NVhCOiClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUeuOlIh|ryP M13WTFIzPzR7M{my
A2780 cells M1[2UmN6fG:2b4jpZ4l1gSCjc4PhfS=> MYO5OkBp NWjtNpI6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF04Njd5IN88US=> NYOwdHoyOjJ5NEmzPVI>
A549 cells NVvGO4I3S3m2b4TvfIlkcXS7IHHzd4F6 M3HnUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[nliTWTUJIF{e2G7LDDJR|UxRThwNDFOwG0> NIrUcIEzPjB5OUC5NC=>
Lipo cells M2jPcGN6fG:2b4jpZ4l1gSCjc4PhfS=> MlPQPVYhcA>? MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMbZBwKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OD62OEDPxE1? M37MVFIzPzR7M{my
A253 cells M{LlPWN6fG:2b4jpZ4l1gSCjc4PhfS=> NH[3UIk6PiCq MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlU{KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OD65NkDPxE1? NED1do0zOjd2OUO5Ni=>
HT-29 cells M4HrT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIXhOGJIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJXC1{OTDj[YxteyxiSVO1NF06NjVizszN MnP4NlM1ODNyOES=
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850C cells MkPER5l1d3SxeHnjbZR6KGG|c3H5 MXu5OkBp NVjCb5dVS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hQDVyQzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVEyNjB7IN88US=> MWqyNlc1QTN7Mh?=
HL60 cells NWq3[YtKS3m2b4TvfIlkcXS7IHHzd4F6 MnfYO|IhcA>? MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3QyKGG|c3H5JIlvKHC{ZYPlcoNmKG:oIEKwKUBHSlNuIFnDOVA:OTRwMzFOwG0> MYmyNlkxOTh7NR?=
MSTO-211H cells NFzLe|FRem:uaX\ldoF1cW:wIHHzd4F6 Mn\0O|IhcA>? MnzaRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPU2TPMVIyOUhiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JJRzgXCjbjDicJVmKGG|c3H5MEBKSzVyPUKzMlMh|ryP NYPp[4F3OjN5M{W4Nlk>
HepG2 cells MWfDfZRwfG:6aXPpeJkh[XO|YYm= NIr6[4ozPCCq M3:xc2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBE\WyuVHn0[ZIuSmy3ZVPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKzMlTPxE1? M4qxOlIxPTR3M{O0
KIF5 cells NGjUOG1EgXSxdH;4bYNqfHliYYPzZZk> MUKyOEBp NHzr[nRVd3irY3n0fUBi\2GrboP0JIh2dWGwIFvJSlUh[2WubIOgbY5kfWKjdHXkJIZweiB{NDDodpMh[nliQ3XscHRqfGW{IFLseYUh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjNwN{Wg{txO MXeyNlc4PTR5NB?=

... Click to View More Cell Line Experimental Data

In vivo Tamoxifen (TAM) is widely used for both treatment and prevention of breast cancer. However, it is also carcinogenic in human uterus and rat liver[2]. Tm-inducible Cre-loxP systems are being used in broad areas of research and are providing important biologic insights in tissue development, maintenance, and function. Tamoxifen-induced nuclear localization of Cre recombinase is time- and dose-dependent. Higher doses of tamoxifen induce recombination weeks following administration and Lower doses of tamoxifen induce recombination up to one week following administration. Duration of tamoxifen-induced gene recombination is also dose-dependent. Administration of high Tm doses leads to extended CreER nuclear localization. Tm treatment induces side effects that may have physiologic consequences in Tm-inducible models[4].

Protocol

Cell Research:

[2]

+ Expand
  • Cell lines: MCF7 and T47D
  • Concentrations: 10-6 mol/L
  • Incubation Time: 24, 48, and 96 h
  • Method:

    48 h before the addition of E2 and TAM, cells were washed with 5 mL phosphate-buffered saline (PBS) and then switched to phenol red-free RPMI-1640 containing 10% charcoal-stripped FBS. E2 and TAM were dissolved in absolute ethanol and diluted in the media at 10<sup>-8</sup> mol/L and 10<sup>-6</sup> mol/L, respectively, and then added to the culture medium at 24, 48, and 96 h.


    (Only for Reference)
Animal Research:

[4]

+ Expand
  • Animal Models: Pdx1PB-CreERTm mice
  • Formulation: filter-sterilized corn oil
  • Dosages: --
  • Administration: s.c.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 74 mg/mL (199.18 mM)
Ethanol 74 mg/mL (199.18 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.51
Formula

C26H29NO

CAS No. 10540-29-1
Storage powder
in solvent
Synonyms ICI 46474

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03879174 Not yet recruiting Breast Cancer Female Mediclinic Middle East August 1 2019 Phase 2
NCT03879174 Not yet recruiting Breast Cancer Female Mediclinic Middle East August 1 2019 Phase 2
NCT03879577 Not yet recruiting Breast Cancer|Breast Cancer Female|HER2-positive Breast Cancer|Breast Cancer Stage II|Breast Cancer Stage III University of Chicago April 10 2019 Phase 2
NCT03879577 Not yet recruiting Breast Cancer|Breast Cancer Female|HER2-positive Breast Cancer|Breast Cancer Stage II|Breast Cancer Stage III University of Chicago April 10 2019 Phase 2
NCT03870399 Recruiting Neuroendocrine Tumors|Progesterone Receptor Positive Tumor|Estrogen Receptor Positive Tumor AC Camargo Cancer Center March 13 2019 Phase 2
NCT03870399 Recruiting Neuroendocrine Tumors|Progesterone Receptor Positive Tumor|Estrogen Receptor Positive Tumor AC Camargo Cancer Center March 13 2019 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    Could you tell me if you have a protocol for the use of tamoxifen (S1238) in vivo in mouse? Is there an administration way that is better than another?

  • Answer:

    Tamoxifen (S1238) can be dissolved in 10% ethanol/10% Tween 80/ddH2O at 5 mg/ml clearly. But precipitation will form if the formulation was left at RT for an hour or longer. So we'd suggest that you use it quickly after formulation. Tamoxifen is often administrated via i.p injection.

Estrogen/progestogen Receptor Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID