Tamoxifen (ICI 46474)

Name: Tamoxifen (ICI 46474)
Brand: Selleck Chemicals
SKU: S1238
Rating: 5 (41 reviews)

For research use only.

Catalog No.S1238 Synonyms: (Z)-Tamoxifen, trans-Tamoxifen

41 publications

Tamoxifen (ICI 46474) Chemical Structure

CAS No. 10540-29-1

Tamoxifen (ICI 46474, (Z)-Tamoxifen, trans-Tamoxifen) is a selective estrogen receptor modulator (SERM). Tamoxifen enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis.

Selleck's Tamoxifen (ICI 46474) has been cited by 41 publications

Cell, 2020, S0092-8674(20)31394-5

PubMed: 33147445 ( click the link to review the publication )

Cancer Discov, 2018, 8(3):336-353

PubMed: 29242214 ( click the link to review the publication )

Clin Cancer Res, 2021, 10.1158/1078-0432.CCR-20-3905

PubMed: 34011560 ( click the link to review the publication )

Cancers (Basel), 2021, 13(9)2116

PubMed: 33925607 ( click the link to review the publication )

Cell Death Dis, 2021, 12(6):619

PubMed: 34131114 ( click the link to review the publication )

Cell Death Dis, 2021, 12(4):329

PubMed: 33771975 ( click the link to review the publication )

Front Oncol, 2021, 11:681530

PubMed: 34277427 ( click the link to review the publication )

Neurosci Bull, 2021, 10.1007/s12264-021-00765-x

PubMed: 34490521 ( click the link to review the publication )

PLoS One, 2021, 16(8):e0256299

PubMed: 34403436 ( click the link to review the publication )

Biomedicines, 2021, 9(6)681

PubMed: 34208714 ( click the link to review the publication )

Sci Signal, 2020, 13(663)eaax7119

PubMed: 33443209 ( click the link to review the publication )

Aging Dis, 2020, 9;11(3):523-535

PubMed: 32489699 ( click the link to review the publication )

Bone, 2020, 130:115123

PubMed: 31678498 ( click the link to review the publication )

Front Oncol, 2020, 9:1503

PubMed: 31993373 ( click the link to review the publication )

Exp Cell Res, 2020, 386(1):111713

PubMed: 31705846 ( click the link to review the publication )

Molecules, 2020, 25(15)E3355

PubMed: 32722075 ( click the link to review the publication )

Molecules, 2020, 25(20)E4606

PubMed: 33050377 ( click the link to review the publication )

Drug Test Anal, 2020, 10.1002/dta.2917

PubMed: 32852861 ( click the link to review the publication )

Int J Mol Med, 2020, 45(2):497-509

PubMed: 31894257 ( click the link to review the publication )

bioRxiv, 2020, 2020/9/20.4.7.30734

PubMed: 32511305 ( click the link to review the publication )

J Pharmacol Toxicol Methods, 2020, 106895

PubMed: 32629158 ( click the link to review the publication )

NPJ Breast Cancer, 2020, 6:37

PubMed: 32864429 ( click the link to review the publication )

Mol Cell, 2019, 75(6):1103-1116.e9

PubMed: 31420216 ( click the link to review the publication )

Clin Cancer Res, 2019, 10.1158/1078-0432.CCR-18-4044

PubMed: 31591187 ( click the link to review the publication )
J Cell Physiol, 2019, 10.1002/jcp.28910

PubMed: 31141164 ( click the link to review the publication )

Cancer Sci, 2019, 110(4):1420-1430

PubMed: 30724426 ( click the link to review the publication )

Int J Mol Sci, 2019, 20(7)

PubMed: 30974833 ( click the link to review the publication )

J Cell Biochem, 2019, 120(8):12966-12976

PubMed: 31001849 ( click the link to review the publication )

Steroids, 2019, 145:23-31

PubMed: 30776377 ( click the link to review the publication )

Med Sci Monit, 2019, 25:8905-8912

PubMed: 31760402 ( click the link to review the publication )

NPJ Precis Oncol, 2019, 03:18

PubMed: 31341951 ( click the link to review the publication )

Cell Physiol Biochem, 2018, 47(6):2199-2215

PubMed: 29975948 ( click the link to review the publication )

Neoplasia, 2018, 20(8):857-870

PubMed: 30025229 ( click the link to review the publication )

Environ Pollut, 2018, 234:846-854

PubMed: 29248852 ( click the link to review the publication )

Biosci Rep, 2018, 38(6)

PubMed: 30061174 ( click the link to review the publication )

Oncogene, 2017, 36(16):2255-2264

PubMed: 27748766 ( click the link to review the publication )

Oncotarget, 2017, 8(46):80109-80123

PubMed: 29113288 ( click the link to review the publication )

Oncotarget, 2017, 8(63):106721-106739

PubMed: 29290984 ( click the link to review the publication )

Oncotarget, 2015, 6(4):2315-30

PubMed: 25537513 ( click the link to review the publication )

Oncogene, 2014, 33(42):4985-96

PubMed: 24166501 ( click the link to review the publication )

University of North Texas, 2014, Timothy E. Richardson

PubMed: ( click the link to review the publication )

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description

Targets

estrogen receptor ^[1]

In vitro

TAM treatment inhibits significantly MCF7 cell proliferation. Low doses of TAM are able to induce structural chromosomal aberrations (deletions, isochromosomes, translocations, and dicentric chromosomes) in both ER+ and ER- breast cancer cells. This genotoxic effect is higher in those cell lines with HER2 gene amplification^[2]. Whereas TAM at lower concentrations (0.1-1 μM) induces a cell-cycle arrest, pharmacological concentrations (above 5 μM) of TAM have been found to induce apoptosis of breast cancer cells. 5 μM TAM rapidly induced sustained activation of ERK1/2 in ER-positive breast cancer cell lines (MCF-7 and T47D)^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
MDA-MB-435 cells	MV\GeY5kfGmxbjDhd5NigQ>?			NYDPd\|NtUW6qaXLpeIlwdiCxZjDNSGEuVUJvNEO1JIJz\WG\|dDDjZY5k\XJiY3XscEBxem:uaX\ldoF1cW:wLDDJR\|UxRTBwMEWg{txO	NVzzVG5GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS5O\|E5QTlpPkG0PVcyQDl7PD;hQi=>
MDA-MB-231 cells	MojYSpVv[3Srb36gZZN{[Xl?			MV;Jcohq[mm2aX;uJI9nKGW\|dILv[4VvKHKnY3XweI9zKG6nZ3H0bZZmKE2GQT3NRk0zOzFiYoLlZZN1KGOjbnPldkBk\WyuIIDyc4xq\mW{YYTpc44tKEmFNUC9NE4xQCEQvF2=	NFPTeYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEm3NVg6QSd-MUS5O\|E5QTl:L3G+
MCF7	NXG4ZVZLSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M3jVUlczKGi{cx?=	NUTOPVRiSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEGldHn2bZR6KD1iND62JO69VS5?	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzlzOEiyNEc,OTd7MUi4NlA9N2F-
MCF7	M2e2TmFvfGmnc4Tyc4dmdmmlIHHzd4F6		M3q0Nlk3KGi{cx?=	NHfRZnZCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGS{dXegcIV3\WxiY3H1d4lv\yCrbnjpZol1cW:wIH;mJFExOCCyTTDFNk1mdmijbnPl[EBk\WyuIIDyc4xq\mW{YYTpc44hfG9ibHX2[Ywh\XG3aY\hcIVvfCC2bzC1NEBxVSCHMjDh[pRmeiB7NjDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7IE2gNE4yKM7:TT6=	NV\JO4k1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OFM3OjBpPkG5PVQ{PjJyPD;hQi=>
T47D	MlX0RY51cWW\|dILv[4VvcWNiYYPzZZk>		M3LxSVk3KGi{cx?=	Mn\DRY51cWW\|dILv[4VvcWNiYXP0bZZqfHliaX6gbJVu[W5iVES3SEBk\WyuczDhd5Nme3OnZDDhd{BlenWpIHzleoVtKGOjdYPpcochcW6qaXLpeIlwdiCxZjCxNFAheE1iRUKt[Y5p[W6lZXSgZ4VtdCCycn;sbYZmemG2aX;uJJRwKGyndnXsJIVyfWm4YXzlcpQhfG9iNUCgdG0hTTJiYX\0[ZIhQTZiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gSB;IECuNUDPxE1w	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl2M{[yNEc,OTl7NEO2NlA9N2F-
MCF7	MYXBcpRq\XO2cn;n[Y5q[yCjc4PhfS=>		MV25OkBpenN?	MoL6RY51cWW\|dILv[4VvcWNiYXP0bZZqfHliaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{BlenWpIHzleoVtKGOjdYPpcochcW6qaXLpeIlwdiCxZjCxNFAheE1iRUKt[Y5p[W6lZXSgZ4VtdCCycn;sbYZmemG2aX;uJJRwKGyndnXsJIVyfWm4YXzlcpQhfG9iMUCgdG0hTTJiYX\0[ZIhQTZiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gSB;IECuNkDPxE1w	NX62NFBmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OFM3OjBpPkG5PVQ{PjJyPD;hQi=>
MCF7	M3nxTGFvfGmnc4Tyc4dmdmmlIHHzd4F6		NFXjUoE6PiCqcoO=	NInvbXFCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGS{dXegcIV3\WxiY3H1d4lv\yCrbnjpZol1cW:wIH;mJFExOCCyTTDFNk1mdmijbnPl[EBk\WyuIIDyc4xq\mW{YYTpc44hfG9ibHX2[Ywh\XG3aY\hcIVvfCC2bzCxJJBOKEV{IHHmeIVzKDl4IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiQXP0bZZqfHliPTCwMlQh\|ryPLh?=	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl2M{[yNEc,OTl7NEO2NlA9N2F-
T47D	MmfJRY51cWW\|dILv[4VvcWNiYYPzZZk>		MoDVPVYhcHK\|	Mk\MRY51cWW\|dILv[4VvcWNiYXP0bZZqfHliaX6gbJVu[W5iVES3SEBk\WyuczDhd5Nme3OnZDDhd{BlenWpIHzleoVtKGOjdYPpcochcW6qaXLpeIlwdiCxZjCxNFAheE1iRUKt[Y5p[W6lZXSgZ4VtdCCycn;sbYZmemG2aX;uJJRwKGyndnXsJIVyfWm4YXzlcpQhfG9iMUCgdG0hTTJiYX\0[ZIhQTZiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gSB;IECuO{DPxE1w	NGPiU\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm0N\|YzOCd-MUm5OFM3OjB:L3G+
T47D	MUTBcpRq\XO2cn;n[Y5q[yCjc4PhfS=>		M3nS[\|k3KGi{cx?=	M4DKPGFvfGmnc4Tyc4dmdmmlIHHjeIl3cXS7IHnuJIh2dWGwIGS0O2Qh[2WubIOgZZN{\XO\|ZXSgZZMh\HK3ZzDs[ZZmdCClYYXzbY5oKGmwaHnibZRqd25ib3[gNVAxKHCPIFWyMYVvcGGwY3XkJINmdGxicILvcIln\XKjdHnvckB1dyCuZY\lcEBmeXWrdnHs[Y51KHSxIEGgdG0hTTJiYX\0[ZIhQTZiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gSB;IEmg{txONg>?	MkHjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7NEO2NlAoRjF7OUSzOlIxRC:jPh?=
HepG2	M3\kWmN6fG:2b4jpZ4l1gSCjc4PhfS=>		NEOzfoUzPCCqcoO=	Mn\hR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgNlQhcHK\|IHL5JJJme2G8dYLpckBlgWVvYnHz[YQh\my3b4Lld4NmdmOnIHHuZYx6e2m\|LDDDR\|UxKD1iMkSuO{DPxE1w	M1\kSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyM{KyO\|U1Lz5\|MEOyNlc2PDxxYU6=
HEK293	MUHDfZRwfG:6aXPpeJkh[XO\|YYm=		MYiyOEBpenN?	Mmn3R5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSC{ZYPhfpVzcW5iZInlMYJie2WmIH\seY9z\XOlZX7j[UBidmGueYPpd{whS0N3MDC9JFM6NjRizszNMi=>	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN{Mke1OEc,OzB\|MkK3OVQ9N2F-
MCF-7	NGnkNohEgXSxdH;4bYNqfHliYYPzZZk>			MoL2R5l1d3SxeHnjbZR6KGGpYXnud5QhVUOILUegZ4VtdHNiaX6geIhmKGGkc3XuZ4Uhd2ZiMTD1UUBGOiBqZYP0doFlcW:uKTygR5l1d3SxeHnjbZR6KD1iOT64JO69VS5?	MnTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTB6OUOzNkc,QTB6OUOzNlww[T5?
MCF-7	NUTpbHRFS3m2b4TvfIlkcXS7IHHzd4F6			MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBOS0ZvNzDj[YxteyCrbjDwdoV{\W6lZTDv[kAyKHWPIFWyJEhme3S{YXTpc4wqNCCFeYTveI95cWOrdImgQUAyOyEQvF2u	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86ODh7M{OyK\|46ODh7M{OyQE9iRg>?
MCF-7	NXLpdZh2TnWwY4Tpc44h[XO\|YYm=			MUfF[oZm[3SrdnWg[I9{\SCob4KgX\|NJZS1iZYP0doFlcW:uIHHnZYlve3RicILvcIln\XKjdHnvckBw\iCPQ1[tO{Bk\WyuczygSWM2OCB;IECuNFAxOTFizszNMi=>	NYPvWGc5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2PVY5OSd-Mke2PVY5OTxxYU6=
Ishikawa	MUXGeY5kfGmxbjDhd5NigQ>?			NYLoc\|h{S2:wY3XueJJifGmxbjDv[kBkd22yb4Xu[EBz\XG3aYLl[EB1dyCrbnT1Z4UhPTBiJTDv[kB1cGVibXH4bY12dSC\|dHnteYxifGmxbjDv[kBidGujbHnu[UBxcG:\|cHjheIF{\SCjY4Tpeol1gSCrbjDJd4hqc2G5YTDj[YxteyxiRVO1NEA:KDBwMEOzJO69VS5?	M2TGblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh{MEG1PFcoRjh{MEG1PFc9N2F-
MCF-7	NXLyNmdWTnWwY4Tpc44h[XO\|YYm=			NEfSdodG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IF3DSk04KGK{ZXHzeEB1fW2xcjDj[YxteyC3c3nu[{BOS0ZvNzDhd5NigS5uIFXDOVAhRSByLkCxJO69VS5?	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh{NUmzOUc,OTJ6MkW5N\|U9N2F-
ScN2a-cl3	MX\GeY5kfGmxbjDhd5NigQ>?		MWq1JIRigXN?	MVvJcohq[mm2aX;uJI9nKFKPTDDwdolwdiCycn;0[YlvKGmwZnXjeIVlKGmwIH3veZNmKGSrdnnkbY5oKFOlTkLhMYNtOyClZXzsd{BmgHC{ZYPzbY5oKG[3bHygcIVv\3SqIH3veZNmKFC{UDDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hd2ZiUILQd4MhdGW4ZXygZYZ1\XJiNTDkZZl{KGK7IFXMTXNCNCCHQ{WwJF0hPC5\|OTFOwG0v	M4HifFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUizOVg6Lz5{NEG4N\|U5QTxxYU6=
NCI-H460	MWfBcpRq[2GwY3XyJIF{e2G7		MYeyOEBpenN?	NGXpc5FCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUmNKNUh2NkCgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFOUQjDhd5NigSxiR1m1NEA:KDRwNEig{txONg>?	NVqxXHNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3N\|MxQDVpPkG5O\|M{ODh3PD;hQi=>
DU145	NWnBTFdISW62aXPhcoNmeiCjc4PhfS=>		MnvZNlQhcHK\|	Mn;oRY51cWOjbnPldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTVNVQ2KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDTVmIh[XO\|YYmsJGdKPTBiPTC2MlA4KM7:TT6=	NIjHfFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUezN\|A5PSd-MUm3N\|MxQDV:L3G+
SKOV3	MVvBcpRq[2GwY3XyJIF{e2G7		M1iyeVI1KGi{cx?=	M4jsOGFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{Bi\nSncjCyOEBpenNiYomgV3JDKGG\|c3H5MEBIUTVyIE2gOk41KM7:TT6=	NYnXUI06RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3N\|MxQDVpPkG5O\|M{ODh3PD;hQi=>
MCF7	MYXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MofhOFghcHK\|	MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDpcoN2[mG2ZXSge4l1cCByLkCwN{BvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEGgcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFUmKG[ndHHsJIJwfmmwZTDz[ZJ2dSxiR1m1NEA:KDJwMkmwPFch\|ryPLh?=	M3zOZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN\|I4PTxxYU6=
MCF7	NX64TXpmSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NI\oT3E1QCCqcoO=	M17pbWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDlfJBz\XO\|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIlv[3WkYYTl[EB4cXSqIECuNFYhdk1ib3[g[ZN1emGmaX;sJIFv\CByLkC3JI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSCrbjDwdoV{\W6lZTDv[kBn\XSjbDDic5ZqdmVic3XyeY0h[W6mIF71V4VzfW1uIFfJOVAhRSB{Lkm1NVIyKM7:TT6=	MnfsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO\|UoRjJzNUGzNlc2RC:jPh?=
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MCF-7	MVjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?			NVTCdlJKSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkhcW5iTVPGMVchcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsd{whUUN3MDC9JFIvOyEQvF2u	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ3OEi3OUc,OTV4NUi4O\|U9N2F-
MDA-MB 231	MX7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?			NYHUPVVZSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkhcW5iTVTBMW1DKDJ\|MTDoeY1idiCkcnXhd5Qh[2GwY3XyJINmdGy\|LDDJR\|UxKD1iMUCuOkDPxE1w	NWr3Smd{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW2OVg5PzVpPkG1OlU5QDd3PD;hQi=>
MCF7	MV3BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?	Mn3GN{Bu\y:tZz;kZZk>	MXO0PEBpenN?	MlflRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDNR2Y4KGOnbHzzJIF1KDNibXevb4cw\GG7IHHmeIVzKDR6IHjyd{whUUN3MDC9JFUvODlizszNMi=>	NXL5eZc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O\|k{OzdpPkG2PVc6OzN5PD;hQi=>
MCF7	NIjJU41CdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NIf4SI8zPCCqcoO=	NFjTbJVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OCB;IECuOVgh\|ryPLh?=	MnnrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{N{KyOVYoRjF6MkeyNlU3RC:jPh?=
insect cells	NEWwUmhHfW6ldHnvckBie3OjeR?=			NGG1[4NFcXOybHHj[Y1mdnRib3[g[ox2d3Knc3PlbY4hdGGkZXzl[EBme3S{YXTpc4wh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDFVoFteGijIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCrboPlZ5Qh[2WubIOgZpkh\my3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeTygTWM2OCB;IECuNFch\|ryPLh?=	MoLjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6M{WxO\|YoRjF6OEO1NVc3RC:jPh?=
insect cells	NHLNSHVHfW6ldHnvckBie3OjeR?=			M{HuOGRqe3CuYXPlcYVvfCCxZjDmcJVwemW\|Y3XpckBt[WKnbHXkJIV{fHKjZHnvcEBnem:vIHj1cYFvKHKnY3;tZolv[W62IFXSZoV1[SCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhcW6\|ZXP0JINmdGy\|IHL5JIZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[XluIFnDOVAhRSByLkG3JO69VS5?	NUfKfZNnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4N\|UyPzZpPkG4PFM2OTd4PD;hQi=>
Ishikawa	MmTYRY51cWW\|dILv[4VvcWNiYYPzZZk>		MUK3NkBpenN?	Mm\BRY51cWW\|dILv[4VvcWNiYXP0bZZqfHliaX6gbJVu[W5iSYPobYtif2FiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBme3S{b3flck1qdmS3Y3XkJIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGGldHn2bZR6KGGodHXyJFczKGi{czygTWM2OCB;IECuNVch\|ryPLh?=	NEjlOI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEizOVE4Pid-MUi4N\|UyPzZ:L3G+
MCF7	M{XVemFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M1LRUFczKGi{cx?=	NEDhUHdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIV{fHKxZ3XuJJJm[2WydH;yJIRmeGWwZHXueEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTC0MlEzKM7:TT6=	MkHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6M{WxO\|YoRjF6OEO1NVc3RC:jPh?=
T47D	M4fOfmFvfGmnc4Tyc4dmdmmlIHHzd4F6			MXPBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCWNEfEJINmdGy\|IHX4dJJme3Orbneg[ZN1em:pZX6gdoVk\XC2b4KgZZN{\XO\|ZXSgZZMh\XO2cn;n[Y4u\GWyZX7k[Y51KHS{YX7zZ5JqeHSrb36gZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR\|UxKD1iMD6xJO69VS5?	Mn\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzM{GyOFgoRjF7MUOxNlQ5RC:jPh?=
T47D	NXrkbWxpT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MYO3NkBpenN?	M4L5[Wdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJIV{fHKxZ3XuMYRmeGWwZHXueEBVPDeGIHPlcIx{KGGodHXyJFczKGi{czygTWM2OCB;IEGg{txONg>?	M2\0NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUOxNlQ5Lz5zOUGzNVI1QDxxYU6=
MCF7	M1jYPWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MofBOFghcHK\|	NGjFcXVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOg[ZhxemW\|c3nu[{Bme3S{b3flckBz\WOncITvdkBi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNVAh\|ryPLh?=	NES2UpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUSyN\|M2Pid-MUm0NlM{PTZ:L3G+
MDA-MB-231	NUL4[JpGSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MV60PEBpenN?	MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKGW\|dILv[4VvKHKnY3XweI9zKGSnZnnjbYVvfCCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzOCEQvF2u	NVLKbnl6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NlM{PTZpPkG5OFI{OzV4PD;hQi=>
MCF7	MlPIRY51cWOjbnPldkBie3OjeR?=		M{DTclE5KGi{cx?=	MkTPRY51cWOjbnPldkBi[3Srdnn0fUBi\2GrboP0JIV{fHKxZ3XuMZBwe2m2aY\lJIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiMUigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGyJO69VS5?	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR2NkmzNEc,OTl2NE[5N\|A9N2F-
MDA-MB-231	NYXpZZM4SW62aXPhcoNmeiCjc4PhfS=>		MmCxNVghcHK\|	MX;BcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6tdI9{cXSrdnWgbJVu[W5iTVTBMW1DNTJ\|MTDj[YxteyCjZoTldkAyQCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOjRizszNMi=>	M4\pTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NES2PVMxLz5zOUS0Olk{ODxxYU6=
MCF7	MWfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		Mnu1OFghcHK\|	NF7o[WxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEeuOlUh\|ryPLh?=	MlP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5NEC2OlcoRjF7N{SwOlY4RC:jPh?=
MDA-MB-231	M1jS[GFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MlTFOFghcHK\|	NGfGOmRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDlwOE[g{txONg>?	M1yweVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{SwOlY4Lz5zOUe0NFY3PzxxYU6=
MCF7	Mn7qR5l1d3SxeHnjbZR6KGG\|c3H5			M3LZNGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIFlemmjbYnjbY4uKHKnc3nzeIFvfCCPQ1[3JINmdGy\|IHL5JHdUXC1zIHHzd4F6NCCLQ{WwJF0hOTBwOTFOwG0v	M3r1fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEO2NlMxLz5zOUizOlI{ODxxYU6=
MCF7	Mn7ZR5l1d3SxeHnjbZR6KGG\|c3H5			NV:4UFlGS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hfGGvb4jp[oVvNXKnc3nzeIFvfCCPQ1[3JINmdGy\|IHL5JHdUXC1zIHHzd4F6NCCLQ{WwJF0hOTFwMTFOwG0v	NH;yTWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUizOlI{OCd-MUm4N\|YzOzB:L3G+
MCF7	MU\DfZRwfG:6aXPpeJkh[XO\|YYm=			M1y0eGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[nliV2PUMVEh[XO\|YYmsJGlEPTBiPTCxNU41KM7:TT6=	Mk\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6M{[yN\|AoRjF7OEO2NlMxRC:jPh?=
MDA-MB-231	MlrpR5l1d3SxeHnjbZR6KGG\|c3H5			M2LSVmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN\|Eh[2WubIOgZpkhX1OWLUGgZZN{[XluIFnDOVAhRSBzMj60JO69VS5?	NGrYVm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUizOlI{OCd-MUm4N\|YzOzB:L3G+
MDA-MB-231	MkflRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?			NGTvcopCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNE43PiEQvF2u	Mn[5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUGzPFAoRjJyNEWxN\|gxRC:jPh?=
MCF7	M3zJW2FvfGmycn;sbYZmemG2aY\lJIF{e2G7			M{f1V2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDifUBOXFRiYYPzZZktKEmFNUCgQUAyKM7:TT6=	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR3MUO4NEc,OjB2NUGzPFA9N2F-
HepG2	Ml7yR5l1d3SxeHnjbZR6KGG\|c3H5		MmfENlQhcHK\|	MWrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyOEBpenNiYomgR4VtdFSrdHXyMWJtfWWFZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IEKzMlQh\|ryPLh?=	NF7sdnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEW0OVM{PCd-MkC1OFU{OzR:L3G+
HT-29	MVjDfZRwfG:6aXPpeJkh[XO\|YYm=		Mlz0NlQhcHK\|	MUTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjCyOEBpenNiYomgR4VtdFSrdHXyMWJtfWWFZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IEO4MlYh\|ryPLh?=	NXXK[21YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1OFU{OzRpPkKwOVQ2OzN2PD;hQi=>
MCF7	NYjWemYyS3m2b4TvfIlkcXS7IHHzd4F6		MX6yOEBpenN?	NETld5VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUA5NjlizszNMi=>	NX35ZpVFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NFU1PzBpPkKwOlA2PDdyPD;hQi=>
HEK293	NH20S\|ZEgXSxdH;4bYNqfHliYYPzZZk>		NGLmOZAzPCCqcoO=	MmrZR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzMDFOwG0v	NUnoO3NKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NFU1PzBpPkKwOlA2PDdyPD;hQi=>
MDA-MB-231	Ml76R5l1d3SxeHnjbZR6KGG\|c3H5		MorKNlQhcHK\|	NULDcZRpS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNVAh\|ryPLh?=	M3O5b\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNkC1OFcxLz5{ME[wOVQ4ODxxYU6=
HeLa	NEnDW\|JEgXSxdH;4bYNqfHliYYPzZZk>		MV2yOEBpenN?	NWPtd\|dOS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOTJwNTFOwG0v	NXfkVXB7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NFU1PzBpPkKwOlA2PDdyPD;hQi=>
MCF7	NWHLdHZRSW62aXPhcoNmeiCjc4PhfS=>			NY\Xd\|BNSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh\XiycnXzd4lv\yCnc4Tyc4dmdiC{ZXPldJRweiCkeTDNWHQh[XO\|YYmsJGlEPTBiPTCxNU45KM7:TT6=	MlTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7NUGwN\|UoRjJyOUWxNFM2RC:jPh?=
MCF7	MWXDfZRwfG:6aXPpeJkh[XO\|YYm=			MXXDfZRwfG:6aXPpeJkh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIJ6KE2WUzDhd5NigSxiSVO1NEA:KDNzIN88UU4>	Mof4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4OECwOlQoRjJzNkiwNFY1RC:jPh?=
HEK293	Mk\ZRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M1LtdlQ5KGi{cx?=	NH;6RnNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjFT\|I6OyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNVAh\|ryPLh?=	Mlj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
MCF7	MojHRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MWO0PEBpenN?	MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMUKuOFgh\|ryPLh?=	M4\1[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEexPFEzLz5{MUi3NVgyOjxxYU6=
MDA-MB-231	MmDFRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NXjLWoZDPDhiaILz	MkOzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTVTBMW1DNTJ\|MTDj[YxteyCneIDy[ZN{cW6pIHXzeJJw\2WwIILlZ4VxfG:{IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzOD65JO69VS5?	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh5MUixNkc,OjF6N{G4NVI9N2F-
Ishikawa	MWLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M1fQdFQ5KGi{cx?=	NFm5RYdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFnzbIls[XejIHPlcIx{KGW6cILld5NqdmdiZYP0do9o\W5icnXj[ZB1d3JiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDJ{LkWg{txONg>?	MmrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
MCF7	NUDsbpVTS3m2b4TvfIlkcXS7IHHzd4F6		NYHuTnh1PDhiaILz	MVrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO\|YYmsJGlEPTBiPTCwMlAzPyEQvF2u	M3rqOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEeyNFQ2Lz5{MkS3NlA1PTxxYU6=
AH109	MX\GeY5kfGmxbjDhd5NigQ>?		MkHkNlQhcHK\|	M{H0[WFvfGGpb37pd5Qh[WO2aY\peJkh[XRiRWLi[ZRiKHKnY3XweI9zKEyERDDlfJBz\XO\|ZXSgbY4hgWWjc4SgRWgyODliY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBqdnSncnHjeIlwdiC5aYToJHNTSzFiYX\0[ZIhOjRiaILzJIJ6KGGucHjhMYdidGGldH;zbYRie2ViYYPzZZktKEmFNUCgQUAyNjZ4IN88UU4>	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
AH109	MlrzSpVv[3Srb36gZZN{[Xl?		MVyyOEBpenN?	NHrSXJNCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFXSZYxxcGFicnXj[ZB1d3JiTFLEJIV5eHKnc4Pl[EBqdiC7ZXHzeEBCUDFyOTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGmwdHXyZYN1cW:wIIfpeIghW1KFMTDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG\|ZTDhd5NigSxiSVO1NEA:KDJwNUSg{txONg>?	NWnqdIxVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
MCF7	M4HjfWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MoDtNlQhcHK\|	NGnod2VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKH[rYXLpcIl1gSCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOTlwNUSg{txONg>?	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
DLD1	MVvDfZRwfG:6aXPpeJkh[XO\|YYm=		MVK5OkBpenN?	NGT5VGVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFVERzIHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUA1Njd6IN88UU4>	MnjwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N\|kzRC:jPh?=
MCF7	MYrDfZRwfG:6aXPpeJkh[XO\|YYm=		MUm5OkBpenN?	NGXrdIFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUA4NjFizszNMi=>	NGXjXXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
NIH/3T3	NXnaZ3d1S3m2b4TvfIlkcXS7IHHzd4F6		MmHUPVYhcHK\|	MnnGR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUmlJNzOWMzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hPy5{NjFOwG0v	M4XCNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S5N\|kzLz5{Mke0PVM6OjxxYU6=
518A2	NIjtU\|dEgXSxdH;4bYNqfHliYYPzZZk>		MVO5OkBpenN?	NVr4eXlIS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hPTF6QUKgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDdwNkKg{txONg>?	M13wTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S5N\|kzLz5{Mke0PVM6OjxxYU6=
A2780	NV\T[GxMS3m2b4TvfIlkcXS7IHHzd4F6		M3zrSFk3KGi{cx?=	M{XMNGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUA4Njd5IN88UU4>	NEXRWoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
Lipo	M4iyUWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NGe2WJM6PiCqcoO=	MmfnR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUIlxdyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG\|c3H5MEBKSzVyIE2gPE43PCEQvF2u	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
A253	M4jTdGN6fG:2b4jpZ4l1gSCjc4PhfS=>		MnHYPVYhcHK\|	NGTpTnREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjV\|IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUA5Njl{IN88UU4>	NIn6SnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
A549	NWDpVIlQS3m2b4TvfIlkcXS7IHHzd4F6		MWS5OkBpenN?	MmjDR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG\|c3H5MEBKSzVyIE2gPU43PiEQvF2u	MnfwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N\|kzRC:jPh?=
850C	NIXKbZpEgXSxdH;4bYNqfHliYYPzZZk>		NVTiSXdkQTZiaILz	M{fKSWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFg2OENiY3XscJMh[W[2ZYKgPVYhcHK\|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFEyNjB7IN88UU4>	NH71bXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
KIF5	M376enRwgGmlaYT5JIF{e2G7		MUiyOEBpenN?	NVXpXYN[XG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLTWY2KGOnbHzzJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IFPlcIxVcXSncjDCcJVmKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxKD1iMkOuO\|Uh\|ryPLh?=	M12y[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{e1OFc1Lz5{Mke3OVQ4PDxxYU6=
MCF7	MkTFR5l1d3SxeHnjbZR6KGG\|c3H5		NYPDXYppPzJiaILz	NGXmcIREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBYW1RzIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFIxLSCIQmOsJGlEPTBiPTC4MlYh\|ryPLh?=	NH\ERVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmwNVg6PSd-MkK5NFE5QTV:L3G+
HL60	NGewfFdEgXSxdH;4bYNqfHliYYPzZZk>		M{DMblczKGi{cx?=	MnzWR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC3NkBpenNiYomgW3NVOSCjc4PhfUBqdiCycnXz[Y5k\SCxZjCyNEUhTkKVLDDJR\|UxKD1iMUSuN{DPxE1w	NELST209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmwNVg6PSd-MkK5NFE5QTV:L3G+
MCF7	MkTiR5l1d3SxeHnjbZR6KGG\|c3H5		MmHwOFghcHK\|	MmfQR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNVEvPDRizszNMi=>	M1S0[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEKyNlgyLz5{M{CyNlI5OTxxYU6=
MDA-MB-231	MkLhR5l1d3SxeHnjbZR6KGG\|c3H5		NWXIRVJ5PDhiaILz	MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzMj60NUDPxE1w	M3HXXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEKyNlgyLz5{M{CyNlI5OTxxYU6=
MCF7	NGfTb\|FEgXSxdH;4bYNqfHliYYPzZZk>		MkXMOFghcHK\|	MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwJF0hOS56MjFOwG0v	NHrBb4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{GyN\|AyPyd-MkOxNlMxOTd:L3G+
MCF7	NEPme2NCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M2DBNVQ5KGi{cx?=	NID2eJNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEWuOUDPxE1w	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6N{C1O{c,OjN{OEewOVc9N2F-
DU145	MkTCRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MlW1OFghcHK\|	NHy1WZFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTVNVQ2KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTC1MlUh\|ryPLh?=	M2PRPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mki3NFU4Lz5{M{K4O\|A2PzxxYU6=
HepG2	NYfN[XptSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MoS0OFghcHK\|	MYHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEincFeyJINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB4LkOg{txONg>?	MorXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OEewOVcoRjJ\|Mki3NFU4RC:jPh?=
PC3	NH7pcnNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NV76V2Z1PDhiaILz	MkHuRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCSQ{OgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDFyIN88UU4>	NUDsPIE3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPFcxPTdpPkKzNlg4ODV5PD;hQi=>
NIH/3T3	Mm\WRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NYKzU4w2PDhiaILz	M{m5T2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViTlnIM\|NVOyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNVEvPCEQvF2u	MnnoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OEewOVcoRjJ\|Mki3NFU4RC:jPh?=
MDA-MB-231	NXu0clNmSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MmW3OFghcHK\|	NFLpU\|lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDFzLkSg{txONg>?	NF3tdms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O\|A2Pyd-MkOyPFcxPTd:L3G+
A549	NYnl[Ww{SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NYrPO3ZkPDhiaILz	M3;mNWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMUKg{txONg>?	M1XCV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mki3NFU4Lz5{M{K4O\|A2PzxxYU6=
T47D	NEm5bVlCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MkX3OFghcHK\|	MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFR2N1SgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDF\|IN88UU4>	NHPkblc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O\|A2Pyd-MkOyPFcxPTd:L3G+
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GBM1	Mlq3RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MYS3NkBpenN?	M3rQfWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwJF0hPy53IN88UU4>	MoDXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ\|NUW1N\|IoRjJ4M{W1OVMzRC:jPh?=
MCF7	MVrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?			MoGwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPQ1[3JINmdGy\|LDDJR\|UxKD1iMD6wOVc2PCEQvF2u	MoXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MEewNVIoRjJ4NEC3NFEzRC:jPh?=
MCF	M2r6O2Z2dmO2aX;uJIF{e2G7			M1TQ[GFvfGGpb37pd5Qh[WO2aY\peJkh[XRicILv[4V{fGW{b37lJJJm[2WydH;yJIlvKGi3bXHuJG1ETiClZXzsd{Bie3Onc4Pl[EBieyCnc4TyZYRqd2xvaX7keYNm\CC{ZXPldJRweiC{ZYPwc45{\SxiSVO1NEA:KDBwMUK4PFIh\|ryPLh?=	NVzke4tjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NFcxOTJpPkK2OFA4ODF{PD;hQi=>
MCF7	NHXkSItHfW6ldHnvckBie3OjeR?=			MXjBcpRi\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEhemWlZYD0c5IhcW5iaIXtZY4hVUOINzDj[YxteyxiSVO1NEA:KDFyIN88UU4>	NV3rbVVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NFcxOTJpPkK2OFA4ODF{PD;hQi=>
MCF7	NHHa[WNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NYS2eXJOPzJiaILz	NI\oclVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IESuOEDPxE1w	NIPq[Yo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki5OlcxPid-Mk[4PVY4ODZ:L3G+
MRC5	M{XFPWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NYnoOZlYPCC2bzC3JIRigXN?	NEHScnBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IHPlcIx{KGG\|c3Xzd4VlKGG\|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQhfG9iNzDkZZl{KGK7IFHsZY1ieiCEbIXlJJN1[WmwaX7nJIJie2WmIH\seY9zd22ndILpZ{BidmGueYPpd{whUUN3MDC9JFExNjlizszNMi=>	MkTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7MkKyNlYoRjJ4OUKyNlI3RC:jPh?=
MCF7	M4nXfWFvfGmycn;sbYZmemG2aY\lJIF{e2G7			NHLO[lhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOsJGlEPTBiPTCxN{44KM7:TT6=	NXrSfHJkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexOVU1PjlpPkK3NVU2PDZ7PD;hQi=>
MDA-MB-231	M{LPNmFvfGmycn;sbYZmemG2aY\lJIF{e2G7			M2XSeGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ\|MTDj[YxteyxiSVO1NEA:KDF5Lkmg{txONg>?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF3NUS2PUc,OjdzNUW0Olk9N2F-
MCF7	NH;wfZVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NGj0cXI1QCCqcoO=	NITmbphCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgbY5kfWKjdHXkJIZweiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iNT6zJO69VS5?	M3nxSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUe2PVQ1Lz5{N{G3Olk1PDxxYU6=
Ishikawa	MXjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		Mn;SOFghcHK\|	MU\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEm\|aHnrZZdiKGOnbHzzJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IE[g{txONg>?	M1z1PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUe2PVQ1Lz5{N{G3Olk1PDxxYU6=
MDA-MB-231	M1;Zd2FvfGmycn;sbYZmemG2aY\lJIF{e2G7		NYLSc4czPDhiaILz	MVPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMhcW6ldXLheIVlKG[xcjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNVMvQSEQvF2u	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF5Nkm0OEc,OjdzN{[5OFQ9N2F-
MCF7	MlL6R5l1d3SxeHnjbZR6KGG\|c3H5			MULDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIJ6KHKnc3H6eZJqdiCvaXPyc5Bt[XSnIHHzd4F6NCCLQ{WwJF0hPi57OTFOwG0v	NYfKTW9iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkeyNlgyPTlpPkK3NlI5OTV7PD;hQi=>
MCF7	NWq0OmhTS3m2b4TvfIlkcXS7IHHzd4F6			M1jhfGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHL5JJJme2G8dYLpckBucWO{b4DsZZRmKGG\|c3H5MEBKSzVyIE2gNVkvOzNizszNMi=>	MkDaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd\|MUG4PVQoRjJ5M{GxPFk1RC:jPh?=
MCF7	NVzaRnpMSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MnPSO\|IhcHK\|	NFfNdnVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVTNXCxc3n0bZZmKGi3bXHuJG1ETjdiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IH3lZZN2emWmIHHmeIVzKDd{IHjyd{BjgSCDbHHtZZJDdHWnIHHzd4F6NCCLQ{WwJF0hPC5zIN88UU4>	MkTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd2MEewN\|AoRjJ5NEC3NFMxRC:jPh?=
insect cells	NWm1cWRiTnWwY4Tpc44h[XO\|YYm=		M2\nc\|QhcHK\|	NHLITVVFcXOybHHj[Y1mdnRib3[gSox2d3Kvb37lJGVUOiCJcnXlckBnem:vIHj1cYFvKHKnY3;tZolv[W62IH\1cIwhdGWwZ4ToJGVT[WyyaHGg[ZhxemW\|c3XkJIlvKGmwc3XjeEBk\WyuczDt[YF{fXKnZDD1dEB1dyB2IHjyd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGG\|c3H5MEBKSzVyIE2gNE4zOjJizszNMi=>	NGPjR2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[0O\|M4PSd-Mke2OFc{PzV:L3G+
SH-SY5Y	MXnGeY5kfGmxbjDhd5NigQ>?		MnnJNUBpeg>?	MljNTY5pcWKrdHnvckBw\iCSS1OgbY4hcHWvYX6gV2guW1l3WTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFCPQT3zeIlufWyjdHXkJG1CWkONUzDwbI9{eGixconsZZRqd25icILlbY5kfWKjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KFCPQT3zeIlufWyjdHnvckBnd3JiMUWgcYlveyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZMtKEmFNUCgQUAzOCEQvF2u	NV7Ud5dYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OFc{PzVpPkK3OlQ4Ozd3PD;hQi=>
FADU	M3\oV2FvfGmlYX7j[ZIh[XO\|YYm=		M3PVPVQ5KGi{cx?=	M1PQR2FvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDGRWRWKGOnbHzzJI1m[XO3cnXkJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO\|YYmsJGlEPTBiPTC1MlM6KM7:TT6=	Mn;kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4N{G0PVcoRjJ5NkexOFk4RC:jPh?=
DLD1	NUjMelZPSW62aXPhcoNmeiCjc4PhfS=>		NHT6OHc1QCCqcoO=	NETlU5dCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gSGxFOSClZXzsd{Bu\WG\|dYLl[EBi\nSncjC0PEBpenNiYomgV3JDKGG\|c3H5MEBKSzVyIE2gNVEvODZizszNMi=>	MkXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4N{G0PVcoRjJ5NkexOFk4RC:jPh?=
A549	M4LwVWFvfGmlYX7j[ZIh[XO\|YYm=		M{T1[lQ5KGi{cx?=	NWrYSoZ3SW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMhdWWjc4Xy[YQh[W[2ZYKgOFghcHK\|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFE4NjNizszNMi=>	NVPnZlRCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2O\|E1QTdpPkK3OlcyPDl5PD;hQi=>
DU145	MV3BcpRq[2GwY3XyJIF{e2G7		MYW0PEBpenN?	Mmj2RY51cWOjbnPldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTVNVQ2KGOnbHzzJI1m[XO3cnXkJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO\|YYmsJGlEPTBiPTCxPE4xPyEQvF2u	MnHyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4N{G0PVcoRjJ5NkexOFk4RC:jPh?=
MCF7	MkXuRY51cWOjbnPldkBie3OjeR?=		NHvndIw1QCCqcoO=	MmPnRY51cWOjbnPldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFXSMZBwe2m2aY\lJG1ETjdiY3XscJMhdWWjc4Xy[YQh[W[2ZYKgOFghcHK\|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFE5NjF6IN88UU4>	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzZ5MUS5O{c,Ojd4N{G0PVc9N2F-
MCF7	NUTTSnFTTnWwY4Tpc44h[XO\|YYm=		MUWyOEBpenN?	NV;Ud2UzSW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoFteGijIHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDld5Rzd2enbj3pcoR2[2WmIITyZY5{[3KrcITpc44heHKnaX7jeYJifGWmIH;2[ZJvcWeqdDDmc4xtd3enZDDifUBme3S{b3flckBi\GSrdHnvckBu\WG\|dYLl[EBi\nSncjCyOEBpenNiYomg[JVidCCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGlEPTBiPTCxMlYxPSEQvF2u	NY\YPIZxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyPVY{QThpPkK4Nlk3Ozl6PD;hQi=>
insect cells	MXrGeY5kfGmxbjDhd5NigQ>?			MmjFSIl{eGyjY3Xt[Y51KG:oIH\seY9z\XOlZXnuMYxi[mWuZXSg[ZN1emGmaX;sJEhndHWxcn;tc45mMSCocn;tJIh2dWGwIILlZ49u[mmwYX70JIZ2dGxvbHXu[5RpKHWwdHHn[4VlKGW\|dILv[4VvKHKnY3XweI9zKGKndHGg[ZhxemW\|c3XkJIlvKGmwc3XjeEBk\WyuczDifUBndHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7LDDJR\|UxKD1iMD6xPFgh\|ryPLh?=	NVHpXmxFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0NlY6OzFpPkK4OFI3QTNzPD;hQi=>
Ishikawa	MV3GeY5kfGmxbjDhd5NigQ>?		NV[xWIhJPzJiaILz	MX7BcpRi\2:waYP0JIFkfGm4aYT5JIF1KGW\|dILv[4VvKHKnY3XweI9zKGGucHjhM4JmfGFiaX6gbJVu[W5iSYPobYtif2FiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGG\|c3H5MEBKSzVyIE2gNE4zQCEQvF2u	M1jod\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEK2PVMyLz5{OESyOlk{OTxxYU6=
MCF7	NUXMSJR2SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NFz2RYY4OiCqcoO=	NUTadY4{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBGWi2mZYDlcoRmdnRiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPC5zIN88UU4>	M13QflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEK2PVMyLz5{OESyOlk{OTxxYU6=
MCF7	NHzGdmdEgXSxdH;4bYNqfHliYYPzZZk>		MUK1JIRigXN?	MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA2KGSjeYOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hPC52IN88UU4>	NEe0Xow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OESyOlk{OSd-Mki0NlY6OzF:L3G+
MCF7	M{jDOmFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NHTR[JE1QCCqcoO=	M1Ppe2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgSXIheG:\|aYTpeoUhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNVEvOzVizszNMi=>	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4MEixPUc,Ojh2NkC4NVk9N2F-
Ishikawa	MWPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NVryO5hnPDhiaILz	M{jRXWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSYPobYtif2FiY3XscJMh[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFE3NjR5IN88UU4>	NY\lTlVuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlA5OTlpPkK4OFYxQDF7PD;hQi=>
MCF7	MmPHR5l1d3SxeHnjbZR6KGG\|c3H5		M1H4WFQ5KGi{cx?=	MkflR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDJzLkW3JO69VS5?	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDVyNUWzOkc,Ojh3MEW1N\|Y9N2F-
HeLa	NGq1Z4dEgXSxdH;4bYNqfHliYYPzZZk>		NVu2[XBSOjRiaILz	M3HRfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB6Lki1JO69VS5?	NIjFTYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEizPFY6PCd-Mki4N\|g3QTR:L3G+
HeLa	NWnibXJOTnWwY4Tpc44h[XO\|YYm=		NWHQ[XRrOjRiaILz	MV\BcpRi\2:waYP0JIFkfGm4aYT5JIF1KG[3bHygcIVv\3SqIFXSZYxxcGFiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gbJVu[W5iSHXMZUBk\WyuczDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCHUlWt[JJqfmWwIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvOzRzIN88UU4>	Mm\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6OEK1NFIoRjJ6OEiyOVAzRC:jPh?=
HeLa	MnPxSpVv[3Srb36gZZN{[Xl?		MWGyOEBpenN?	NH\MPZhCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IH\1cIwhdGWwZ4ToJGVT[mW2YTCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBpfW2jbjDI[WxiKGOnbHzzJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IFXSSU1lemm4ZX6gcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOS53MzFOwG0v	NHLlWGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEi4NlUxOid-Mki4PFI2ODJ:L3G+
MCF7	NHTndplEgXSxdH;4bYNqfHliYYPzZZk>		MVq3NkBpenN?	M1mydmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JJJme2G8dYLpckBj[XOnZDDmcJVwemW\|Y3XuZ4Uh[XO\|YYmsJGlEPTBiPTC3Mlk1KM7:TT6=	Ml;kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7Mke3PVUoRjJ6OUK3O\|k2RC:jPh?=
insect cells	M3y4W2Z2dmO2aX;uJIF{e2G7		M4TSd\|IhcHK\|	Mn\TSIl{eGyjY3Xt[Y51KG:oIH\seY9z\XOlZX70JIV{fHKxZ3XuJIxq\2GwZDDmdo9uKHKnY3;tZolv[W62IHj1cYFvKEWUYXzwbIEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{BqdmO3YnH0[YQh\m:{IEKgbJJ{KGK7IIDvcIFzcXqjdHnvckwhUUN3MDC9JFIvPjdizszNMi=>	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl2MkGxN{c,Ojh7NEKxNVM9N2F-
MCF7	NXrURpNRS3m2b4TvfIlkcXS7IHHzd4F6			NWHLWG5PS3m2b4TvfIlkcXS7IHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTCxOE4{PSEQvF2u	MlvUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NEKxNVMoRjJ6OUSyNVE{RC:jPh?=
Ishikawa	MVPDfZRwfG:6aXPpeJkh[XO\|YYm=			MnXBR5l1d3SxeHnjbZR6KGmwIHj1cYFvKEm\|aHnrZZdiKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCpcn;3eIghcW6ldXLheIVlKG[xcjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNlAvPjVizszNMi=>	Mme2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NEKxNVMoRjJ6OUSyNVE{RC:jPh?=
MCF7	NUjNVFR6SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M4j6fVQ5KGi{cx?=	M1fGUGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iOD6xNkDPxE1w	NX;pfYI3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwOVQ{PTlpPkK5NFU1OzV7PD;hQi=>
MDA-MB-231	MVHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NFm1SYQ1QCCqcoO=	MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFkvQTZizszNMi=>	NFK3UYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC1OFM2QSd-MkmwOVQ{PTl:L3G+
HEK293	Mn;lRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M4K0eVQ5KGi{cx?=	MXjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFjFT\|I6OyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNlAvQTNizszNMi=>	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB3NEO1PUc,OjlyNUSzOVk9N2F-
MCF7	M3ryOGN6fG:2b4jpZ4l1gSCjc4PhfS=>			MoDuR5l1d3O2YYTpZ{Bi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOsJGlEPTBiPTCyMlch\|ryPLh?=	NGLMWI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUKwO\|M{PSd-MkmyNFc{OzV:L3G+
MCF7	M4fZdmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MUC3NkBpenN?	NV63ZoYxT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPS5\|IN88UU4>	NXnhVoZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNlA4QDlpPkK5NlIxPzh7PD;hQi=>
MCF7	MWrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M{X3e\|Q5KGi{cx?=	NGPZNZhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEG5MlUh\|ryPLh?=	NVPiTWVoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0PFI6PDRpPkK5OFgzQTR2PD;hQi=>
MCF7	MUnGeY5kfGmxbjDhd5NigQ>?		NE\0OIszPCCqcoO=	NX23W5llUW6mdXP0bY9vKG:oIIPlcIVkfGm4ZTDld5Rzd2enbjDy[YNmeHSxcjDhcJBp[SCmZXfyZYRifGmxbjDpckBpfW2jbjDNR2Y4KGOnbHzzJIhiemKxcnnu[{BVUy2HUlWtUJVkKGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBme3S{YXTpc4wucW6mdXPl[EB1emGwc3PybZB1cW:wYXygZYN1cX[rdImgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFEvPjB3IN88UU4>	NWDrUY1[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OlI4OzdpPkK5OVYzPzN5PD;hQi=>
insect cells	MVfGeY5kfGmxbjDhd5NigQ>?		MV6yJIhzew>?	M4XnNmRqe3CuYXPlcYVvfCCxZjDGcJVwem2xbnWgSXMzKEe{ZXXuJIZzd21iZoXscEBt\W6pdHigbJVu[W5iRWKtZYxxcGFiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyCjZoTldkAzKGi{czDifUBndHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7LDDJR\|UxKD1iMT60NkDPxE1w	M3f0eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi3NlIyLz5{OUW4O\|IzOTxxYU6=
MCF7	MVXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MmrFOFghcHK\|	M1e0OmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMUWuOFYh\|ryPLh?=	MlfwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEeyNlEoRjJ7NUi3NlIyRC:jPh?=
Ishikawa	NID5fYtCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NYnUPXdHPDhiaILz	NEPTTlJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFnzbIls[XejIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzPi53MjFOwG0v	MkfHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEeyNlEoRjJ7NUi3NlIyRC:jPh?=
MRC5 SV2	MVPDfZRwfG:6aXPpeJkh[XO\|YYm=		M4DadFczKGi{cx?=	NGH4ZmVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IGPWNkBk\WyuczDh[pRmeiB5MjDodpMh[nlicnXzZZp2emmwIHT5[U1j[XOnZDDmcJVwemmvZYTybYMh[W6jbInzbZMtKEmFNUCgQUAyOC5{IN88UU4>	NWjmc4dNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2NFQ2PDFpPkK5OlA1PTRzPD;hQi=>
MCF7	M1y2T2N6fG:2b4jpZ4l1gSCjc4PhfS=>		NEXCTVU1KGi{cx?=	M2GwfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMheHKnaX7jeYJifGWmIH\vdkA1KGi{czDmc4xtd3enZDDifUBqdmO3YnH0bY9vKGmwIHPvcZBwfW6mIH\y[YUhdWWmaXGg[o9zKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB6Lk[xJO69VS5?	NUnJXpBMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2NFU5ODhpPkK5OlA2QDB6PD;hQi=>
DLD1	MWnDfZRwfG:6aXPpeJkh[XO\|YYm=		M2LKeFQhcHK\|	NWDaOYw3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTEyGMTDj[YxteyCycnXpcoN2[mG2ZXSg[o9zKDRiaILzJIZwdGyxd3XkJIJ6KGmwY4XiZZRqd25iaX6gZ49ueG:3bnSg[pJm\SCvZXTpZUBnd3JiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEG2MlMh\|ryPLh?=	M3\IUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkC1PFA5Lz5{OU[wOVgxQDxxYU6=
A549	NYjOWnhFS3m2b4TvfIlkcXS7IHHzd4F6		NELX[HY1KGi{cx?=	MX;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJJBz\WmwY4XiZZRm\CCob4KgOEBpenNiZn;scI94\WRiYomgbY5kfWKjdHnvckBqdiClb33wc5Vv\CCocnXlJI1m\GmjIH\vdkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOTdwMzFOwG0v	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZyNUiwPEc,Ojl4MEW4NFg9N2F-
HeLa	MXnGeY5kfGmxbjDhd5NigQ>?		Mo\kOE42KGi{cx?=	NXjSUnh4UW6qaXLpeIlwdiCxZjDFZo9t[X[rcoXzJIdtgWOxcILveIVqdi:vYYTybZgheHKxdHXpckBXWDRyIHXueJJ6KGmwIHj1cYFvKEinTHGgZ4VtdHNiYX\0[ZIhPC53IHjyd{Bj\XSjLXzhZ5RidWG\|ZTDy[ZBwenSncjDhd5NigSxiSVO1NEA:KDBwN{Og{txONg>?	NITYU4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[yOFM5Pyd-Mkm2NlQ{QDd:L3G+
MRC5	Mnn4R5l1d3SxeHnjbZR6KGG\|c3H5		M3PoO\|czKGi{cx?=	MX3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNVmM2KGOnbHzzJIF{e2W\|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBz\XOjeoXybY4h[XO\|YYmsJGlEPTBiPTCxNEDPxE1w	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ5MkC0NUc,Ojl4N{KwOFE9N2F-
MCF7	M3rNcmFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MlW3NlQhcHK\|	NED1[opCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEeuNFkh\|ryPLh?=	MnHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB\|NkC5OVIoRjNyM{[wPVUzRC:jPh?=
MDA-MB-231	NFn0NXhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NGTxSVEzPCCqcoO=	MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[W[2ZYKgNlQhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFQ4NjFizszNMi=>	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN4MEm1Nkc,OzB\|NkC5OVI9N2F-
HeLa	MW\GeY5kfGmxbjDhd5NigQ>?		NILLPG0yQCCqcoO=	NWSyNlVSTGm\|cHzhZ4Vu\W62IH;mJHszNDRuNjy3MVNJZWW\|dILh[IlwdCCocn;tJIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhmVGFiY3XscJMh[W[2ZYKgNVghcHK\|IHL5JIxqeXWrZDDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCNaTC9JFAvODNzMUmg{txONg>?	NVnoVFU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NlE1QTJpPkKwOlIyPDl{PD;hQi=>
HEK293	NHfrVGJHfW6ldHnvckBie3OjeR?=		MUmxMlUhcHK\|	MkHMSIl{eGyjY3Xt[Y51KG:oIGuzTH1NW0RiZoLvcUBpfW2jbjC1THQ3KHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNU42KGi{czDifUBtcXG3aXSgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiS3mgQUAyNjB2MTFOwG0v	NULQc\|JkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1N\|cyPTNpPkKyOVM4OTV\|PD;hQi=>
ScN2a-cl3	NXvzOIF1S3m2b4TvfIlkcXS7IHHzd4F6		NWrhdoZ7PSCmYYnz	MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W\|ZTDkbZZq\GmwZzDTZ24z[S2lbEOgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPSCmYYnzJIJ6KGOjbHPlbY4uSU1ic4ThbY5qdmdvYnHz[YQh\my3b4Lld4NmdmOnIHHzd4F6NCCOQ{WwJF0hPC56OTFOwG0v	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF6M{W4PUc,OjRzOEO1PFk9N2F-
MCF7	NEjVOI1HfW6ldHnvckBie3OjeR?=	M1X2eF4yOCC3TR?=		M1HTeGFod26rc4SgZYN1cX[rdImgZZQhTVKjbIDoZUBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGUzNWmwZIXj[YQhdHWlaX\ldoF{\SCpZX7lJJRz[W6\|YXP0bZZifGmxbjDpckBOS0Z5IHPlcIx{KGG2IE6xNEB2VQ>?	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjd\|NUGyNEc,OTZ5M{WxNlA9N2F-
HEK293	MYXGeY5kfGmxbjDhd5NigQ>?		NULSXoY6OzBibXnudy=>	NWDMfYR1W3SrbYXsZZRqd25ib3[gS2ZRNXSjZ3fl[EBpfW2jbjDQUGQzKGW6cILld5Nm\CCrbjDoeY1idiCKRVuyPVMh[2WubIOgZZN{\XO\|ZXSgZZMheGixc4DoZZRq\HmuYoX0ZY5wdC2dZEndJJBzd2S3Y4Tpc44h[W[2ZYKgN\|AhdWmwczDifUBu[XO\|IIPw[YN1em:vZYTybYMh[W6jbInzbZM>	M4HQPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUO2PVc2Lz5zOUGzOlk4PTxxYU6=
MDA-MB-231	NHGwSGdEgXSxdH;4bYNqfHliYYPzZZk>		M1iwZ\|Mh\GG7cx?=	M{jkWGN6fG:\|dHH0bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHHmeIVzKDNiZHH5d{BjgSCvaXPyc5RqfGW{IHHzd4F6	M{\rUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUm4OVU2Lz5{MEW5PFU2PTxxYU6=
HeLa	NGj0bmRHfW6ldHnvckBie3OjeR?=	M2OxSFEhfU1?	MUO0PEBpenN?	NHj2O5dCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHj1cYFvKEWUYXzwbIEh\XiycnXzd4VlKGmwIHj1cYFvKEinTHGgZ4VtdHNiY3;lfJBz\XO\|aX7nJGVTTS2HMXKtUJVkKGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4Yh\XO2cnHkbY9tNWmwZIXj[YQhfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44h[XRiMTD1UUBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	Ml7mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4MkG0PVIoRjJyNkKxOFkzRC:jPh?=
MCF7	NYrPbWU{S3m2b4TvfIlkcXS7IHHzd4F6	MlXJOkB2VQ>?	NYHk[INDQTZiaILz	NHLGc4NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h[XRiNjD1UUBi\nSncjC5OkBpenNiYomgUXRVKGG\|c3H5	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ{MUS5Nkc,OjB4MkG0PVI9N2F-
HeLa	NHrHR4FHfW6ldHnvckBie3OjeR?=			MmXIRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckB4cWymIIT5dIUhTVKjbIDoZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWOYTDj[YxteyClbz3lfJBz\XO\|aX7nJGVTTSCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHS{YX7zZYN1cX[jdHnvckBi[3Srdnn0fUBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ3OUiwNUc,OjB4NUm4NFE9N2F-
HeLa	NGnLdpdHfW6ldHnvckBie3OjeR?=			NHLTUI9VemGwc3HjeIl3[XSrb36gc4YhcHWvYX6ge4lt\CC2eYDlJGVT[WyyaHGg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmVGFiY3XscJMh[29vZYjwdoV{e2mwZzDBVE0yKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ3OUiwNUc,OjB4NUm4NFE9N2F-
HeLa	M4nMd2Z2dmO2aX;uJIF{e2G7			Mni5RY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckB4cWymIIT5dIUhTVKkZYThJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\UyjIHPlcIx{KGOxLXX4dJJme3OrbnegSXJGKGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ3OUiwNUc,OjB4NUm4NFE9N2F-
HeLa	NX[wPWxGTnWwY4Tpc44h[XO\|YYm=			MmD2RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjD3bYxlKHS7cHWgSXJj\XSjIHX4dJJme3OnZDDpckBpfW2jbjDI[WxiKGOnbHzzJINwNWW6cILld5NqdmdiQWCtNUBie3Onc4Pl[EBieyC2cnHud4FkfGm4YYTpc44h[WO2aY\peJkh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	MlX3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4NUm4NFEoRjJyNkW5PFAyRC:jPh?=
MCF7	MlftRY51cWW\|dILv[4VvcWNiYYPzZZk>	MmPRNlAhdk1?	NGjJZXc1QCCqcoO=	MljjRY51cWW\|dILv[4VvcWNiYXP0bZZqfHliaX6gbJVu[W5iRWKtdI9{cXSrdnWgUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJFE4[mW2YTDld5Rz[WSrb3ytbY5lfWOnZDDz[YNz\XSnZDDhcItidGmwZTDwbI9{eGijdHHz[UBi[3Srdnn0fUBifCB{MDDuUUBi\nSncjC0PEBpenNiYomgdIhwe3CqYT3sbYdpfCC{ZYDvdpRmeiClaHXtbYx2dWmwZYPj[Y5k\SCjc4PhfS=>	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOThyMEi1PUc,OjF6MEC4OVk9N2F-
Escherichia coli C41 DE3	MkLwSpVv[3Srb36gZZN{[Xl?	MmG5OlAhfU1?	M4PlWFExKG2rboO=	NX33OlFjUW6jY4TpeoF1cW:wIH;mJGN[WDKENjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGM1OSCGRUOgZ4VtdHNiYYPz[ZN{\WRiYYOgcY9lcW[rZXSgZZBwNVB2NUCgNmI3KGGmZIXjeEBnd3KvYYTpc44h[XRiNkCgeW0h[W[2ZYKgNVAhdWmwczDifUBGW0lvTFOtUXMh[W6jbInzbZMhcW5icILld4Vv[2Vib3[gUmFFWEh?	Mn;TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7NEKzNVcoRjJ{OUSyN\|E4RC:jPh?=
Escherichia coli C41 DE3	M2XSUmZ2dmO2aX;uJIF{e2G7	M3;RVlExOCC3TR?=	MX6zNEBucW6\|	MY\JcoFkfGm4YYTpc44hd2ZiQ2nQNmI3KCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQ{SxJGRGOyClZXzsd{Bie3Onc4Pl[EBieyC2conweIlkKHCncITp[IUhOTh6RljZVWRSTTF7NDD3bZRpKHOrdHWgc4Yh[WSmdXP0JIZwem2jdHnvckBtd2OjbHn6[YQhfG9iR3zuNVk{KGG2IEGwNEB2VSCjZoTldkA{OCCvaX7zJIJ6KEyFLV3TM21UKGGwYXz5d4l{KGmwIIDy[ZNmdmOnIH;mJG5CTFCK	M2jrOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUSyN\|E4Lz5{Mkm0NlMyPzxxYU6=
Escherichia coli C41 DE3	MkfSSpVv[3Srb36gZZN{[Xl?	NUfnXFVuPjBidV2=		MWfJcoFkfGm4YYTpc44hd2ZiQ2nQNmI3KCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQ{SxJGRGOyClZXzsd{Bie3Onc4Pl[EBieyCvb3Tp[olm\CCjcH:tVFQ2OCB{Qk[gZYRlfWO2IH\vdo1ifGmxbjDheEA3OCC3TTDifUBGW0lvTFOtUXMh[W6jbInzbZMhcW5icILld4Vv[2Vib3[gUmFFWEhiYX7kJGdUUEWH	M1zOeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUSyN\|E4Lz5{Mkm0NlMyPzxxYU6=
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HeLa	NYn4bWZjS3m2b4TvfIlkcXS7IHHzd4F6		MWe0PEBpenN?	NIO0PFhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDpckBxemW\|ZX7j[UBw\iBzMDDtUUBPSUNiYX\0[ZIhPDhiaILzJIJ6KFeVVEigZZN{[Xl?	MkfnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkO2OlcoRjJ3MU[zOlY4RC:jPh?=
Neuro2a	MlW3SpVv[3Srb36gZZN{[Xl?	Mmr4NUB2VQ>?		NEnjXplKdmirYnn0bY9vKG:oIHTlcJRiKDhvNzDpd49u\XKjc3WgbY4hTGilcket[IVncWOrZX70JI1wfXOnIF7leZJwOmFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gfplud3O2ZX7vcEBt\X[nbIOgZZQhOSC3TTDifUBNSy2PUz;HR{1OWyCjbnHsfZNqew>?	Mn3OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5OEm2OVcoRjJ4N{i5OlU4RC:jPh?=
Neuro2a	M1fF[2Z2dmO2aX;uJIF{e2G7	MlTFNUB2VQ>?		M2fjUmlvcGmkaYTpc44hd2ZiZHXseIEhQC15IHnzc41memG\|ZTDpckBud3W\|ZTDO[ZVzdzKjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJFcuTEiFIHzleoVteyCjdDCxJJVOKGK7IFzDMW1UN0eFLV3TJIFv[Wy7c3nz	NUPiOXh5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3PFk3PTdpPkK2O\|g6PjV5PD;hQi=>
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Neuro2a	M2frTGZ2dmO2aX;uJIF{e2G7	Mm[3NUB2VQ>?		MnfVTY5pcWKrdHnvckBw\iCmZXz0ZUA5NTdiaYPvcYVz[XOnIHnuJGRp[3J5LXTl[olkcWWwdDDtc5V{\SCQZYXyc\|JiKGOnbHzzJIF{e2W\|c3XkJIF{KGSnY4LlZZNmKGmwIHPoc4xme3Sncn;sJIxmfmWuczDheEAyKHWPIHL5JGxENU2VL1fDMW1UKGGwYXz5d4l{	Mk\ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5OEm2OVcoRjJ4N{i5OlU4RC:jPh?=
Neuro2a	M2XsN2Z2dmO2aX;uJIF{e2G7	M360dFEhfU1?		NWmyZ4gyUW6qaXLpeIlwdiCxZjDk[Yx1[SB6LUegbZNwdWW{YYPlJIlvKESqY4K3MYRm\mmlaXXueEBud3W\|ZTDO[ZVzdzKjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJFcuTEiFIHzleoVteyCjdDCxJJVOKGK7IFzDMW1UN0eFLV3TJIFv[Wy7c3nz	NXvDU3B4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3PFk3PTdpPkK2O\|g6PjV5PD;hQi=>
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Neuro2a	Mlq5SpVv[3Srb36gZZN{[Xl?	MofTNUB2VQ>?		MX7Jcohq[mm2aX;uJI9nKESUMkSgbY4hTGilcket[IVncWOrZX70JI1wfXOnIF7leZJwOmFiY3XscJMh[XO\|ZYPz[YQh[XNiZHXjdoVie2ViaX6gO{1FUENibHX2[Yx{KGG2IEGgeW0h[nliTFOtUXMwT0NvTWOgZY5idHm\|aYO=	M1S4ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{i5OlU4Lz5{Nke4PVY2PzxxYU6=
AsPC1	MV7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?	MmXXNVAhfU1?	NWOzWJRqPDhiaILz	Mly4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCDc2DDNUBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDheEAyOCC3TTDh[pRmeiB2ODDodpMh[nliTWTTJIF{e2G7	M3;xcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OECzNlA1Lz5{NkiwN\|IxPDxxYU6=
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MCF7	MmKwSpVv[3Srb36gZZN{[Xl?	M3LoUFMhfG9iMUCgeW0>	MkDHNlQhcHK\|	NULxS2o4UW6qaXLpeIlwdiCxZjDT[ZQ4NzliaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{B{fXCycnXzd4lwdiCxZjDi[ZRiNWW\|dILh[IlwdC2rbnT1Z4VlKEWURTDwdo9ud3SncjDhZ5RqfmG2aX;uJIF1KDNidH:gNVAhfU1icILlbY5kfWKjdHXkJIZweiBzODDodpMh\m:ubH;3[YQh[nliYnX0ZU1me3S{YXTpc4wh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>?	NVLtZ\|dlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewPFg3PDhpPkK3NFg5PjR6PD;hQi=>
MCF7	M3;nTWZ2dmO2aX;uJIF{e2G7	M3v6S\|MhfG9iMUCgeW0>	NEXsVFczPCCqcoO=	NGPu[IdKdmirYnn0bY9vKG:oIGPleFcwQSCrbjDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJJN2eHC{ZYPzbY9vKG:oIHLleIEu\XO2cnHkbY9tNWmwZIXj[YQheFN{IH3SUmEh\XiycnXzd4lwdiCjdDCzJJRwKDFyIIXNJJBz\WmwY4XiZZRm\CCob4KgNVghcHK\|IH\vcIxwf2WmIHL5JIJmfGFvZYP0doFlcW:uIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZM>	MknmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyOEi2OFgoRjJ5MEi4OlQ5RC:jPh?=
MCF7	NXHpcmVbTnWwY4Tpc44h[XO\|YYm=	NGjnXpgyKHWP		Mo\yTY5pcWKrdHnvckBw\iCHUnHsdIhiKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKDFyIH7NJIV{fHKxZ3XuMYlv\HWlZXSgdJJw\2W\|dHXyc45mKHKnY3XweI9zKGW6cILld5Nqd25iYYSgNUB2VSCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RibXX0bI9l	NW\McYNXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFIyOTNpPkK4PVQzOTF\|PD;hQi=>
MCF7	MmqxSpVv[3Srb36gZZN{[Xl?	MX2xJJVO	NXL0N5BuOTZiaILz	Mn6zTY5pcWKrdHnvckBw\iCHUnHsdIhiKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOg[I94dnKnZ4XsZZRqd25ib3[gNVdj\XSjLXXzeJJi\GmxbD3t[YRq[XSnZDDwV\|IhdVKQQTDlfJBz\XO\|aX;uJIF1KDFidV2gdJJmcW6ldXLheIVlKG[xcjC0JIhzeyCob3zsc5dm\CCkeTCxO4JmfGFvZYP0doFlcW:uIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDF4IHjyd{BjgSCzdXHueIl1[XSrdnWgVnQuWEOUIHHuZYx6e2m\|	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB2NUGzOUc,OjlyNEWxN\|U9N2F-
MCF7	M3TkU2N6fG:2b4jpZ4l1gSCjc4PhfS=>		MmDGNlQhcHK\|	MnzzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUB2eCC2bzCxNFAhfU1iYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigQ>?	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB2NUGzOUc,OjlyNEWxN\|U9N2F-
U2O2	MUHGeY5kfGmxbjDhd5NigQ>?	NX7n[WZkOSC3TR?=	NFPmbHQzOCCqcoO=	NHqzUmJKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFfGVE1nfXOnZDDFVoFteGijIFzCSEApOzBzIITvJFU2OyC{ZYPp[JVmeyliZYjwdoV{e2WmIHnuJIh2dWGwIGWyU\|Ih[2WubIOgZZN{\XO\|ZXSgZZMh\G:5boLl[5Vt[XSrb36gc4YhOTekZYThMYV{fHKjZHnvcE1qdmS3Y3XkJJRz[W6\|Y4LpdJRqd25iYYSgNUB2VSCycnXpcoN2[mG2ZXSg[o9zKDRiaILzJIZwdGyxd3XkJIJ6KEV{IHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDJyIHjyd{BjgQ>?	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB2NUGzOUc,OjlyNEWxN\|U9N2F-
MCF7	MV;D[YxtKGO7Y3zlJIFzemW\|dDDhd5NigQ>?	NHTIW3QyOCC3TR?=	MWqyOEB1dyB2ODDodpM>	MYjD[YxtKGO7Y3zlJIFzemW\|dDDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGGlY4XteYxifGmxbjDheEBIOC:JMTDwbIF{\SCjdDCxNEB2VSCjZoTldkAzPCC2bzC0PEBpenNiYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfS=>	NIn6ZWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC1OFM2QSd-MkmwOVQ{PTl:L3G+
MCF7	MUDGeY5kfGmxbjDhd5NigQ>?	MlLiNUB2VQ>?		NUHWc5NRSW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoFteGijIHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIHXzeJJi\GmxbD3pcoR2[2WmIGDnVkBuWk6DIHX4dJJme3Orb36gZZQhOSC3TTDifUBUYUKUIFfy[YVvKGS7ZTDiZZNm\CCUVD3QR3Ih[W6jbInzbZM>	NH7PUW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS4Nlk1PCd-Mkm0PFI6PDR:L3G+
Ishikawa	MlfMRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	MVi+OFAhfU1?	NEK3bm01QCCqcoO=	MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEm\|aHnrZZdiKGOnbHzzJIF1KD52MDD1UUBi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5	MlTyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OEK5OFQoRjJ7NEiyPVQ1RC:jPh?=
MCF7	MnvzSpVv[3Srb36gZZN{[Xl?	NF3ufW43OCCvZz;r[y=>	MUW1JIRigXNicHXyJJdm\WtiZn;yJFQ5KGSjeYO=	MnfQSI94dnKnZ4XsZZRqd25ib3[gdJJw\2W\|dHXyc45mKHKnY3XweI9zKG2UTlGgcIV3\WxiaX6gUm9FKFOFSVSg[4FudWFibX;1d4UhgGWwb3fyZYZ1\WRid3n0bEBpfW2jbjDNR2Y4KGOnbHzzJIF1KDZyIH3nM4toNCCybzC1JIRigXNicHXyJJdm\WtiZn;yJFQ5KGSjeYOgdI9{fCC2dX3vdkBqdXCuYX70ZZRqd25ibXXhd5Vz\WRiNzDodpMheG:\|dDDsZZN1KGSxc3WgZpkhWlRvUFPSJI1mfGixZB?=	Ml3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NkK3N\|coRjJ7NU[yO\|M4RC:jPh?=
MCF7	MYnBcpRqfHWvb4KgZZN{[Xl?	MXy2NEBu\y:tZx?=	NYrLUHlUPCC5ZXXrdy=>	M1vDUmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgfIVvd2e{YX\0[YQhcW5iboWvcpUhdW:3c3WgcY9l\Wxib3[geIFud3irZnXuMZNmdnOrdHn2[UBjemWjc4SgZ4Fv[2W{IIjlco9oemGodDDpcZBt[W62ZXSge4l1cCBzN3LleIEu\XO2cnHkbY9tKHSrbXWgdoVt\WG\|ZTDw[Yxt\XS\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckB1fW2xcjD2c4x2dWViYYSgOlAhdWdxa3esJJBwKHGmIH\vdkA1KHenZXvz	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB6Nk[yOkc,OzByOE[2NlY9N2F-
insect cells	M3O2NGZ2dmO2aX;uJIF{e2G7	NYDBd206OC5zIITvJFExODByIIXN		MmHnRolv\GmwZzDh[oZqdmm2eTD0c{B2dnSjZ3fl[EBpfW2jbjDy[YNwdWKrbnHueEBnfWyuIHzlcod1cCCHUnHsdIhiKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMhOC5zIITvJFExODByIIXNJIJ6KE[udX;ycY9v\SCHU{KgS5Jm\W5iZHnzdIxi[2WvZX70JIJie2WmIHHzd4F6	NWfNcpJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwPVE6OjBpPkOwNFkyQTJyPD;hQi=>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-AKT / AKT; PubMed: 30759864 Cancers (Basel) MCF-7 cells were treated with 2 μM tamoxifen and harvested at the indicated time point to analyze the expressions of p-AKT and AKT by western blot. ER-α66 / ER-α36 / HER2 / EGFR; PubMed: 25203051 PLoS One Western blot analysis of the expression levels of ER-α66, ER-α36, HER2 and EGFR in ER-positive breast cancer MCF7 (A), T47D (B) and H3396 (C) cells treated with 1 µM of tamoxifen (TAM) for indicated time period. p-AMPK / AMPK / p-ACC / ACC / p-P70S6K / p-S6 / p-4EBP1; PubMed: 27020861 Cancer Res B. Immunoblot of AMPK pathway activation following treatment with 30μM tamoxifen in MCF7 and C. in MDA-MB-231 cells. CD36 / p-ERK / ERK ; PubMed: 30573731 Oncogenesis MCF-7 cells MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h, or with 5 μM Tam for the indicated times. Expression of CD36, p-ERK1/2 and ERK1/2 was determined by western blot.	30759864 25203051 27020861 30573731
Immunofluorescence	CD36; PubMed: 30573731 Oncogenesis MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h. Expression of CD36 was determined by immunofluorescent staining.	30573731

In vivo

Tamoxifen (TAM) is widely used for both treatment and prevention of breast cancer. However, it is also carcinogenic in human uterus and rat liver^[2]. Tm-inducible Cre-loxP systems are being used in broad areas of research and are providing important biologic insights in tissue development, maintenance, and function. Tamoxifen-induced nuclear localization of Cre recombinase is time- and dose-dependent. Higher doses of tamoxifen induce recombination weeks following administration and Lower doses of tamoxifen induce recombination up to one week following administration. Duration of tamoxifen-induced gene recombination is also dose-dependent. Administration of high Tm doses leads to extended CreER nuclear localization. Tm treatment induces side effects that may have physiologic consequences in Tm-inducible models^[4].

Protocol

Cell Research:

^[2]

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Cell lines: MCF7 and T47D
Concentrations: 10^-6 mol/L
Incubation Time: 24, 48, and 96 h
Method:
48 h before the addition of E2 and TAM, cells were washed with 5 mL phosphate-buffered saline (PBS) and then switched to phenol red-free RPMI-1640 containing 10% charcoal-stripped FBS. E2 and TAM were dissolved in absolute ethanol and diluted in the media at 10^-8 mol/L and 10^-6 mol/L, respectively, and then added to the culture medium at 24, 48, and 96 h.

(Only for Reference)

Animal Research:

^[4]

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Animal Models: Pdx1PB-CreER^Tm mice
Dosages: --
Administration: s.c.
(Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	74 mg/mL (199.18 mM)
	Water	Insoluble
	Ethanol	'''74 mg/mL
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 2% DMSO+30% PEG 300+2% Tween 80+ddH2O For best results, use promptly after mixing.	1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	371.51
Formula	C₂₆H₂₉NO
CAS No.	10540-29-1
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	(Z)-Tamoxifen, trans-Tamoxifen
Smiles	CCC(=C(C1=CC=CC=C1)C2=CC=C(C=C2)OCCN(C)C)C3=CC=CC=C3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04200066	Not yet recruiting	Drug: Temozolomide Tamoxifen Maprotiline	Glioblastoma\|Brain Tumor	University of Rochester	May 1 2021	Phase 1
NCT04433494	Not yet recruiting	Drug: TY-302: capsule 25mg/50mg ; Tamoxifen: tablet10mg	Breast Cancer\|Solid Tumor	TYK Medicines Inc	October 2020	Phase 1
NCT04174352	Recruiting	Drug: Tamoxifen\|Diagnostic Test: FES PET/CT	ERα+ Breast Cancer\|ESR1 Gene Mutation	University of Wisconsin Madison	October 20 2020	Early Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
Could you tell me if you have a protocol for the use of tamoxifen (S1238) in vivo in mouse? Is there an administration way that is better than another?
Answer:
Tamoxifen (S1238) can be dissolved in 10% ethanol/10% Tween 80/ddH2O at 5 mg/ml clearly. But precipitation will form if the formulation was left at RT for an hour or longer. So we'd suggest that you use it quickly after formulation. Tamoxifen is often administrated via i.p injection.

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Tamoxifen (ICI 46474)