Etoposide (VP-16)

Catalog No.S1225 Synonyms: VP-16213

For research use only.

Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.

Etoposide (VP-16) Chemical Structure

CAS No. 33419-42-0

Selleck's Etoposide (VP-16) has been cited by 206 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.
Targets
Topo II [2]
(Cell-free assay)
In vitro

Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA, which induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle. [1] Etoposide inhibits the growth of murine angiosarcoma cell line (ISOS-1) in a 5 days-period with IC50 of 0.25 μg/mL. Cell growth of normal murine microvascular endothelial cells (mECs) is less sensitive to Etoposide with IC50 of 10 μg/mL). [2] Etoposide treated for 6 hr inhibits colonies of tetraploid variant of the human leukemic lymphoblast line CCRF-CEM with IC50 of 0.6 μM. [3] Etoposide treated for 2 hr inhibits growth of human pancreatic cancer cell line Y1, Y3, Y5, Y19, YM. YS, and YT with IC50s of 300 μg/mL, 300 μg/mL, 300 μg/mL, 91 μg/mL, 0.68 μg/mL, 300 μg/mL, 300 μg/mL, and 260 μg/mL, respectively. [4] Etoposide exposed for 1 hr inhibits growth of human glioma cell lines CL5, G142, G152, G111, and G5 with IC50 of 8, 9, 9.8, 10, and 15.8 μg/mL respectively for 12 days. Under same condition, the IC90 value is attained in cell lines CL5, G152, G142, and G111 at 26, 27, 32, and 33 μg/mL. Etoposide inhibition of topoisomerase II is homogeneous for each cell. The average inhibition rates are 15%, 21.8%, 31.8%, 41.5%, and 49.5% for 1, 2, 4, 8, and 16 μg Etoposide, respectively. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Kelly M4TtdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTBwMUNihKnDueLCiUCuNFEh|ryP MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl4MEK4Nkc,OjV7NkCyPFI9N2F-
KellyCis83 NHPGWZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml21TWM2OD1yLkG25qCKyrIkgJmwMlAzKM7:TR?= NIXmNFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm2NFI5Oid-MkW5OlAzQDJ:L3G+
SK-N-AS MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH:xcGpKSzVyPUCuNlTjiIoEsfMAjVAvODNizszN Mlq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NkCyPFIoRjJ3OU[wNlgzRC:jPh?=
SK-N-ASCis24 M3zMOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHKdnpvUUN3ME2wMlU46oDLwsJihKkxNjFzIN88US=> MmfBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NkCyPFIoRjJ3OU[wNlgzRC:jPh?=
U87 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nD[|AuPTBizszN M2fpWFQ5KGh? Mn\T[IVkemWjc3XzJINmdGxidnnhZoltcXS7IIfobYNpKGOjbjDi[UBmdmijbnPl[EBjgSC|aXzpZolvcW5? NEPNTlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe1NFI4Oyd-MkW3OVAzPzN:L3G+
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HT-29 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFW4eVYxNjVvMj61JO69VQ>? NUDHNpJkPDkEoHlCpC=> NHLucpRKSzVyPUeuNuKhyrIEoEGuNFTDqM7:TR?= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd2Nke2N{c,OjV5NE[3OlM9N2F-
Caco2 MlS4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUmwMlUuOi53IN88US=> NXqyS|hzPDkEoHlCpC=> NFXoSZpKSzVyPUeuNlbDqMLzwrCxMlY5yqEQvF2= M2fBPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{S2O|Y{Lz5{NUe0Olc3OzxxYU6=
COLO 205 NYDaOnRkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TaWFAvPS1{LkWg{txO Mn\kOFjDqGkEoB?= NFThdoVKSzVyPUGuOlHDqMLzwrCwMlAzyqEQvF2= NXfR[pFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3OFY4PjNpPkK1O|Q3PzZ|PD;hQi=>
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HEK293 Mo\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTwTWM2OD15LkG0xsDDucLiMD6zOuKh|ryP Mn3zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4MEOxNlIoRjJ3NkCzNVIzRC:jPh?=
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HCT15 MlmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1vBXWlEPTB;MD64NeKhyrIEoECuNFHDqM7:TR?= NV64cIJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NFMyOjJpPkK1OlA{OTJ{PD;hQi=>
T47D MkHwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\oTWM2OD1|LkG4xsDDucLiMD6xNeKh|ryP M2TRfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkCzNVIzLz5{NU[wN|EzOjxxYU6=
SMMC-7721 NFr5PXdHfW6ldHnvckBCe3OjeR?= M4jPO|QxKM7:TR?= MkHGOFghcA>? NFi5V2NFVVOR NFHWTY9qdmS3Y3XzJO6{UDKDWDDmc4NqKG[xcn3heIlwdg>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV2NEO2NUc,OjV3NESzOlE9N2F-
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Jurkat NVL0UnJXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHXU2ZkPzMEoHi= M17aNmlEPTB;MT6yxsDDucLiMT61xsDPxE1? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR6NkKxPUc,OjV2OE[yNVk9N2F-
HeLa NXvVWY5WT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXpfoM4OsLiaB?= NE\qbVZKSzVyPUOuPeKhyrIEoEKuN:Kh|ryP M{i0OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEi2NlE6Lz5{NUS4OlIyQTxxYU6=
MCF7  MlG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1ToblUuOTByIN88US=> MonvO{Bl MlO2bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR5Mk[xPUc,OjV2N{K2NVk9N2F-
K562 NETje|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVG3NuKhcA>? NGr3bFJKSzVyPUCuNlnDqM7:TR?= NYjG[WttRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyPFI3PTNpPkK1NlgzPjV|PD;hQi=>
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SH-EP  NXHCc5F[TnWwY4Tpc44hSXO|YYm= M4XTe|IxyqEQvHevcYw> NFTVb2gzPMLiaB?= M4TEUolv[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBmdmSxZ3Xuc5V{KESHUGC= MkfsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{NkG5PFEoRjJ3Mk[xPVgyRC:jPh?=
SCC25 MoqxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7qUIwzPMLiaB?= M1e5Z2lEPTB;NEOuN:KhyrIEoEGuNVLDqM7:TR?= NWXFO5ZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyNlA4OjlpPkK1NlIxPzJ7PD;hQi=>
CAL27 M4nPfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1SzbFI1yqCq M3\yWmlEPTB;NUKuNeKhyrIEoEGuNFnDqM7:TR?= NUex[GZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyNlA4OjlpPkK1NlIxPzJ7PD;hQi=>
FaDu NGG0[|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDTNlTDqGh? MkfkTWM2OD1{NT64PeKhyrIEoEGuNVPDqM7:TR?= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ{MEeyPUc,OjV{MkC3Nlk9N2F-
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HL60 MkPhR5l1d3SxeHnjbZR6KGG|c3H5 MXi3NkBpenN? M{foVGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOiEQvF2u NX7o[FFuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NFc1PzJpPkG4OVA4PDd{PD;hQi=>
HL60 NFH0OGtCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NYq1UGJRPDhiaILz NYLtfY1RSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlMyPSEQvF2u M3vDOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGzPVc1Lz5zOEWxN|k4PDxxYU6=
K562 MWPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M4fMbFQ5KGi{cx?= MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEt3NkKgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDFwMkeg{txONg>? NXvhVm84RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NVQ6PzJpPkG4OVE1QTd{PD;hQi=>
KB MVPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MVq0PEBpenN? MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEuEIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjR{IN88UU4> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDVzNEm3Nkc,OTh3MUS5O|I9N2F-
L02 NV[1emRtSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NVnTW5M3PDhiaILz NEj0eYhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFywNkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMUOg{txONg>? M17UWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUG0PVczLz5zOEWxOFk4OjxxYU6=
DU145 ATCC HTB 81 M4fvfGN6fG:2b4jpZ4l1gSCjc4PhfS=> MmXaO|IhcHK| M3PWUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IFHUR2MhUFSEIEixJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkWg{txONg>? NFjjfI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW1PFQ6OCd-MUi1OVg1QTB:L3G+
K562 ATCC CCL 243 MY\DfZRwfG:6aXPpeJkh[XO|YYm= M3L1UlczKGi{cx?= MVzDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLOVYzKEGWQ1OgR2NNKDJ2MzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPC57IN88UU4> NGXlSlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW1PFQ6OCd-MUi1OVg1QTB:L3G+
MCF7 ATCC HTB 22 NWDteIc6S3m2b4TvfIlkcXS7IHHzd4F6 NHi1PHI4OiCqcoO= MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KEGWQ1OgTHRDKDJ{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAyOi5{IN88UU4> MmnVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
U251 RCB 0641 NHTDV45EgXSxdH;4bYNqfHliYYPzZZk> NYXhRWtrPzJiaILz NWXiSm51S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTJ3MTDSR2IhODZ2MTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC53IN88UU4> NV:4e5Z3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1OVg1QTBpPkG4OVU5PDlyPD;hQi=>
3LL CRL 1642 M3PUVmN6fG:2b4jpZ4l1gSCjc4PhfS=> M4nPVlQ5KGi{cx?= NGfDWZZEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UA{VExiQ2LMJFE3PDJiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFQh|ryPLh?= NWGwZ|NIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1OVg1QTBpPkG4OVU5PDlyPD;hQi=>
L1210 ATCC CCL 219 M1jQOWN6fG:2b4jpZ4l1gSCjc4PhfS=> MVK0PEBpenN? M{PMRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGwyOjFyIFHUR2MhS0OOIEKxPUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD61JO69VS5? M1Tae|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUW4OFkxLz5zOEW1PFQ6ODxxYU6=
SK-Hep1 NWLGSWN6S3m2b4TvfIlkcXS7IHHzd4F6 M2OybmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNUincEGgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOFkvPiEQvF2u MlzRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4M{e2PFgoRjF6NkO3Olg5RC:jPh?=
HL60 NGPsWm5EgXSxdH;4bYNqfHliYYPzZZk> MVTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDBwMjFOwG0v NWLlNG5MRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2OVE4PzFpPkG4OlUyPzdzPD;hQi=>
A549 NHrtZo1EgXSxdH;4bYNqfHliYYPzZZk> M2jpfWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxiZ4Lve5RpKGK7IGPSRkBu\XSqb3SsJGlEPTBiPTC5Mlk5KM7:TT6= NVrsbWp4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi3PFI3PjhpPkG4O|gzPjZ6PD;hQi=>
KB MXXDfZRwfG:6aXPpeJkh[XO|YYm= MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hd2ZiY3XscEBoem:5dHigZpkhW1KEIH3leIhw\CxiSVO1NEA:KDF2LkKg{txONg>? NEPuTWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEe4NlY3QCd-MUi3PFI3Pjh:L3G+
A549 NX6zdpJyS3m2b4TvfIlkcXS7IHHzd4F6 MWm3NkBpenN? NFT0PYxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUA5NjN5IN88UU4> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh2NESyNkc,OTh6NES0NlI9N2F-
KB MUXDfZRwfG:6aXPpeJkh[XO|YYm= MlrlO|IhcHK| MWrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT6yOUDPxE1w M13DN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OES0OFIzLz5zOEi0OFQzOjxxYU6=
AGS MnjKR5l1d3SxeHnjbZR6KGG|c3H5 M3;wOFMh\GG7cx?= M13Ye2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGFIWyClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4{PiEQvF2u MnPVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyMUOwO|QoRjF7MEGzNFc1RC:jPh?=
HL-60 NVf1SHgyS3m2b4TvfIlkcXS7IHHzd4F6 M{DCclMh\GG7cx?= M3vVbGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNNTZyIHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjhizszNMi=> NFTYb249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCxN|A4PCd-MUmwNVMxPzR:L3G+
J82 NYPT[5l3S3m2b4TvfIlkcXS7IHHzd4F6 NWnTXZcxOyCmYYnz M4myOWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGo5OiClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNk45KM7:TT6= NHW3NI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCxN|A4PCd-MUmwNVMxPzR:L3G+
MRC5 MVnDfZRwfG:6aXPpeJkh[XO|YYm= MXizJIRigXN? NGrVbWhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUA{NjlizszNMi=> MoTYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyMUOwO|QoRjF7MEGzNFc1RC:jPh?=
SK-MES-1 Moq5R5l1d3SxeHnjbZR6KGG|c3H5 MmmxN{Bl[Xm| NH7WOoZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2PRWOtNUBk\WyuczDh[pRmeiB|IHThfZMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj61JO69VS5? MlHNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyMUOwO|QoRjF7MEGzNFc1RC:jPh?=
A375 M1HZT2N6fG:2b4jpZ4l1gSCjc4PhfS=> M4fQfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE{PzViY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMz65NkDPxE1w NXzmd3JIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwN|Y2QDRpPkG5NFM3PTh2PD;hQi=>
A549 MUDDfZRwfG:6aXPpeJkh[XO|YYm= M1fJfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iOT61NUDPxE1w NYi5bol3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwN|Y2QDRpPkG5NFM3PTh2PD;hQi=>
HL60 MVPDfZRwfG:6aXPpeJkh[XO|YYm= MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDFwOEOg{txONg>? M3;sbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEO2OVg1Lz5zOUCzOlU5PDxxYU6=
THP1 NF36d4NEgXSxdH;4bYNqfHliYYPzZZk> NUDoe4FiS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXEiSMTDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCyMlE3KM7:TT6= MlSwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyM{[1PFQoRjF7MEO2OVg1RC:jPh?=
A549 MmL5R5l1d3SxeHnjbZR6KGG|c3H5 NV3CeFlzPzJiaILz NUX4VmJsS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPS57IN88UU4> NW[xNZhMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwOVM2OTRpPkG5NFU{PTF2PD;hQi=>
HL-60 NH7xTHhEgXSxdH;4bYNqfHliYYPzZZk> Mn2wO|IhcHK| Mn[0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGwuPjBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOjVizszNMi=> MlniQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyNUO1NVQoRjF7MEWzOVE1RC:jPh?=
U937 M4T5N2N6fG:2b4jpZ4l1gSCjc4PhfS=> NY[1OZRoPzJiaILz NHS2dlJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWQTN5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjl{IN88UU4> M{Xm[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzOVE1Lz5zOUC1N|UyPDxxYU6=
ACHN MVfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnT1PVYhcHK| NUi5c5BWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBR2hPKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCyMlE2KM7:TT6= NHrwS|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC1N|c3Pyd-MUmwOVM4Pjd:L3G+
CCRF-CEM MWrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NXX4VGZWQTZiaILz Mo\TRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCFQ2LGMWNGVSClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNU4zKM7:TT6= NULHW4l[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwOVM4PjdpPkG5NFU{PzZ5PD;hQi=>
CCRF-SB M{D2eGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NFX0bVc6PiCqcoO= M1jzO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ1PSSk1USiClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4yKM7:TT6= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2O{c,OTlyNUO3Olc9N2F-
HeLa NIrWOYdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NFzZV5k6PiCqcoO= MX3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEinTHGgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDNizszNMi=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2O{c,OTlyNUO3Olc9N2F-
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502713 MmTGRY51cWOjbnPldkBie3OjeR?= MVe0PEBpenN? NXvncHppSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJFUxOjdzMzDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOC5zIN88UU4> M2fZbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEe0OFQ1Lz5{MUC3OFQ1PDxxYU6=
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Hep2 MoLJRY51cWOjbnPldkBie3OjeR?= NVjPbFRzPDhiaILz MoPlRY51cWOjbnPldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldFIh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygTWM2OCB;IECuPUDPxE1w M2jZXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEe0OFQ1Lz5{MUC3OFQ1PDxxYU6=
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HT-29 M3XtcWN6fG:2b4jpZ4l1gSCjc4PhfS=> M{jGWFczKGi{cx?= Mn\NR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhT0l3MDC9JFMyNjZ{IN88UU4> M{\ITFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUG1NlExLz5{MUGxOVIyODxxYU6=
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A549 MYnDfZRwfG:6aXPpeJkh[XO|YYm= MXm3NkBpenN? MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlAxOjV4IN88UU4> MoHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzNEWxN|koRjJzMUS1NVM6RC:jPh?=
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KB-7d M2DhUmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 Mly2O|IhcHK| NVHuPZFxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLRk04\CClZXzsd{BmgHC{ZYPzbY5oKE2UUDDh[pRmeiB5MjDodpMh[nlibXX0bJlt\W6nIHLseYUh[XO|YYmsJGlEPTBiPTC0Ok4yKM7:TT6= NH7wT|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG2NFAzOCd-Mk[xOlAxOjB:L3G+
KB-S15 NWe5VngzSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MoXsO|IhcHK| NU\JOIdTSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLRk1UOTViY3XscJMh\XiycnXzd4lv\yCSLXfwNVcxN02GUjDh[pRmeiB5MjDodpMh[nlibXX0bJlt\W6nIHLseYUh[XO|YYmsJGlEPTBiPTCzMlUh|ryPLh?= NFrRfZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG2NFAzOCd-Mk[xOlAxOjB:L3G+
KB-VIN10 NYX0Tm5xSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MYC3NkBpenN? MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEuELW\JUlExKGOnbHzzJIV5eHKnc4PpcochWC2pcEG3NE9OTFJiYX\0[ZIhPzJiaILzJIJ6KG2ndHj5cIVv\SCkbIXlJIF{e2G7LDDJR|UxKD1iMk[uOEDPxE1w M4nFclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MU[wNFIxLz5{NkG2NFAzODxxYU6=
A549 NHixSWhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M1S3U2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUCgQUAyQC5{IN88UU4> Mne0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{MU[wNVgoRjJ4MkG2NFE5RC:jPh?=
HepG2 MYLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MYHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEincFeyJINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFIxNjlizszNMi=> NXi4RoZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yNVYxOThpPkK2NlE3ODF6PD;hQi=>
A549 NF23[FZEgXSxdH;4bYNqfHliYYPzZZk> MUm0PEBpenN? M1fkcmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCrbnP1ZoF1\WRiZn;yJFQ5KGi{czDifUBJd2WlaIP0JIF{e2G7LDDJR|UxKD1iMD60PEDPxE1w MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ{NUmwOUc,OjZ{MkW5NFU9N2F-
DLD1 M4LQWGN6fG:2b4jpZ4l1gSCjc4PhfS=> NH[xbmg1QCCqcoO= MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEUGQyKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCpcn;3eIghcW6ldXLheIVlKG[xcjC0PEBpenNiYomgTI9m[2i|dDDhd5NigSxiSVO1NEA:KDNwMzFOwG0v NGLTdGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkKyOVkxPSd-Mk[yNlU6ODV:L3G+
HepG2 MmK5RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M3XYdVczKGi{cx?= NF;RXolCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTCzNEDPxE1w NVj0VZBDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yPVExOzdpPkK2NlkyODN5PD;hQi=>
MDA-MB-231 MlrBRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGLS[Yc4OiCqcoO= NWHsR4NLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSB|Lk[g{txONg>? M{HjZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkmxNFM4Lz5{NkK5NVA{PzxxYU6=
MDA-MB-435 MVjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnfkO|IhcHK| MlX2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuPDN3IHPlcIx{KGGodHXyJFczKGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUAyNjdizszNMi=> NH7SSHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK5NVA{Pyd-Mk[yPVExOzd:L3G+
PC3 MXnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NXXTV5lWPzJiaILz M1fiNGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFOzJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSBzIN88UU4> NGfo[4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK5NVA{Pyd-Mk[yPVExOzd:L3G+
A549 MYDDfZRwfG:6aXPpeJkh[XO|YYm= MnfFOFghcHK| NF7Ld3NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUA{QC53IN88UU4> NIfMb|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK5NlYzQCd-Mk[yPVI3Ojh:L3G+
DU145 NHHPR2FEgXSxdH;4bYNqfHliYYPzZZk> MljtOFghcHK| MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOU4zOSEQvF2u MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ7Mk[yPEc,OjZ{OUK2Nlg9N2F-
HGC MXXDfZRwfG:6aXPpeJkh[XO|YYm= NYLGdJVrPDhiaILz M3TJT2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhISyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNVAvQSEQvF2u NYDSdFJpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yPVI3OjhpPkK2NlkzPjJ6PD;hQi=>
MDA-MB-231 MkX6R5l1d3SxeHnjbZR6KGG|c3H5 Mlm0OFghcHK| MmfNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iNEmuN|Uh|ryPLh?= M4nLbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkmyOlI5Lz5{NkK5NlYzQDxxYU6=
RWPE1 MmHGR5l1d3SxeHnjbZR6KGG|c3H5 M2izTFQ5KGi{cx?= MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSW3BGOSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNlcvPjRizszNMi=> MmnMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{OUK2NlgoRjJ4MkmyOlI5RC:jPh?=
HCT15 Mlu4R5l1d3SxeHnjbZR6KGG|c3H5 Ml7SNkBl[Xm| NHHUT5BEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzNTDj[YxteyCvZXHzeZJm\CCjZoTldkAzKGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPS53NDFOwG0v NHLZblg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkOzOFQ6QSd-Mk[zN|Q1QTl:L3G+
HeLa Ml[wR5l1d3SxeHnjbZR6KGG|c3H5 M3LrfFIh\GG7cx?= M2jod2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMhdWWjc4Xy[YQh[W[2ZYKgNkBl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFcvPiEQvF2u NV\T[JN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zN|Q1QTlpPkK2N|M1PDl7PD;hQi=>
T47D NEHjV2VEgXSxdH;4bYNqfHliYYPzZZk> MnjJNkBl[Xm| M1LMO2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHQ1P0RiY3XscJMhdWWjc4Xy[YQh[W[2ZYKgNkBl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFkvOjhizszNMi=> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN|NES5PUc,OjZ|M{S0PVk9N2F-
GBM2 NYHj[mppSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M2jsOVczKGi{cx?= M1SxSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwJF0hOS5zIN88UU4> MmflQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|NUW1N|IoRjJ4M{W1OVMzRC:jPh?=
DU145 NUXQSYxJS3m2b4TvfIlkcXS7IHHzd4F6 NVrwNIZsOiCmYYnz NYDnPYRyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTFVzNEWgZ4VtdHNiYX\0[ZIhOiCmYYnzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSBzMz6xJO69VS5? NEPTb3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO2NVc{Pyd-Mk[zOlE4Ozd:L3G+
HCT15 MYrDfZRwfG:6aXPpeJkh[XO|YYm= NEPwfWszKGSjeYO= NWXLZXl3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUWgZ4VtdHNiYX\0[ZIhOiCmYYnzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSB{Lk[g{txONg>? NEjq[|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO2NVc{Pyd-Mk[zOlE4Ozd:L3G+
MCF10A NGPxTlBEgXSxdH;4bYNqfHliYYPzZZk> NVLrb3A{OiCmYYnz MmPOR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHOTCDIHPlcIx{KGGodHXyJFIh\GG7czDifUBES0t6IHHzd4F6NCCLQ{WwJF0hOTBwMUSg{txONg>? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN4MUezO{c,OjZ|NkG3N|c9N2F-
T47D MkHlR5l1d3SxeHnjbZR6KGG|c3H5 MkP4NkBl[Xm| Ml\WR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWFQ4TCClZXzsd{Bi\nSncjCyJIRigXNiYomgR2NMQCCjc4PhfUwhUUN3MDC9JFAvQTRizszNMi=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN4MUezO{c,OjZ|NkG3N|c9N2F-
HepG2 MXrDfZRwfG:6aXPpeJkh[XO|YYm= NY\OcGIyPDhiaILz M2XT[GN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUA{Oy57ODFOwG0v MoDsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUezPVMoRjJ4M{m3N|k{RC:jPh?=
MOLT3 MmTTR5l1d3SxeHnjbZR6KGG|c3H5 MW[0PEBpenN? M3fvXmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1QVFR|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUCgQUAxNjB2MTFOwG0v NYrXNmNMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVc{QTNpPkK2N|k4Ozl|PD;hQi=>
A549 MWPDfZRwfG:6aXPpeJkh[XO|YYm= MkXsOFghcHK| NX21Wno6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nlicnXzZZp2emmwIHHzd4F6NCCLQ{WwJF0hOS55IN88UU4> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7Mki5O{c,OjZ3OUK4PVc9N2F-
DLD1 NYe0UogzS3m2b4TvfIlkcXS7IHHzd4F6 M3fLb|Q5KGi{cx?= M17BdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRNTDFiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KHKnc3H6eZJqdiCjc4PhfUwhUUN3MDC9JFIh|ryPLh?= M{TMT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmyPFk4Lz5{NkW5Nlg6PzxxYU6=
A549 Ml7HRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NVXVdFRPOjRidH:gO|IhcHK| M4\qbmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB{NDD0c{A4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOC56IN88UU4> NHzM[XA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5OVg4PSd-Mk[1PVU5PzV:L3G+
CAKI-1 NI\KXlFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= Mnr2NlQhfG9iN{KgbJJ{ M3LnVmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ1HLTU0yKGOnbHzzJIFnfGW{IEK0JJRwKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSBzLkGg{txONg>? NVO1TodYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVU5PzVpPkK2OVk2QDd3PD;hQi=>
HCT116 M122[GFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGXjRVYzPCC2bzC3NkBpenN? MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczDh[pRmeiB{NDD0c{A4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOy5|IN88UU4> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7NUi3OUc,OjZ3OUW4O|U9N2F-
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Assay
Methods Test Index PMID
Western blot p53 / p-p53 / Acetyl-p53 / TF-IIB ; p53 / p21 / c-Myc / PARP 23800173 20212154
Immunofluorescence COX IV ; γH2AX / pATM ; MDC1 ; pDNA-PKcs ; p53 ; YAP 29221178 19858003 27515249
Growth inhibition assay Cell viability 22615609
In vivo Etoposide administrated as a single agent is found to been ineffective in many xenografts growth, such as Heterotransplanted Hepatoblastoma NMHB1, and NMHB 2, [6] human neuroblastoma xenograft, [7] and human gastrointestinal cancer xenograft, [8] while the dose of 10 mg/kg i.p. Etoposide inhibits murine angiosarcoma cell ISOS-1 tumors in 36% of controls. [2] Etoposide induces tumor immunity in Lewis lung cancer. A single administration of 50 mg/kg Etoposide i.p., induces a 60% survival of C57B1/6 mice injected with Lewis lung cancer cell (3LL) over 60 days. About 40% of these surviving mice reject a subsequent challenge with 3LL, while none of control mice survive beyond 30 days. 3LL cells which have survived an 90% lethal concentration of Etoposide in vitro kill 75% of recipient mice, but 60% surviving mice reject challenge with 3LL. Splenocytes harvested from tumor rejecting mice protect naive mice injected with 3LL. [9]

Protocol (from reference)

Kinase Assay:[5]
  • Topoisomerase II activity assay:

    Nuclear extracts are prepared, and nuclei are isolated. The activity of topoisomerase II is calculated from the percentage of decatenation obtained. Tritiated kinoplast DNA (KDNA 0.22 μg) is used as a substrate. Etoposide and topoisomerase II are incubated for 30 min at 37 ℃ and are stopped with 1% sodium dodecyl sulfate (SDS) and proteinase K (100 μg/mL). The percentages of decatenation and inhibition of topoisomerase II by Etoposide are obtained.

Cell Research:[5]
  • Cell lines: Human glioma cell lines CL5
  • Concentrations: 80 μg/mL
  • Incubation Time: 1 hour
  • Method: After the Etoposide treatment, cells are removed from the dish with phosphate-buffered saline (PBS) containing 0.03% trypsin and 0.27 mM ethylenediaminetetraacetic acid (EDTA) and are diluted into culture dishes in appropriate numbers to yield between 20 and 200 colonies. After 12 days, cultures are fixed with methanol-acetic acid, stained with crystal violet, and scored for colonies containing more than 50 cells. The standard errors are typically less than 15% of the mean value unless otherwise stated.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: Murine angiosarcoma xenografts ISOS-1
  • Dosages: 10 mg/kg
  • Administration: i.p. every day for 5 days from day 7
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(169.9 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

15mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 588.56
Formula

C29H32O13

CAS No. 33419-42-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1OCC2C(O1)C(C(C(O2)OC3C4COC(=O)C4C(C5=CC6=C(C=C35)OCO6)C7=CC(=C(C(=C7)OC)O)OC)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04935255 Not yet recruiting Other: Blood sampling - Pharmacokinetic assessment Testicular Cancer Radboud University August 1 2021 --
NCT04804007 Recruiting Drug: Etoposide Germ Cell Tumor|Non-seminomatous Germ Cell Tumor|Ovarian Germ Cell Tumor Nabil Adra|Indiana University March 3 2021 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Regarding the Etoposide S1225, do you have any data of the inhibition sepcificity of this product? It will inhibit other enzymes other than TOP2A?

Answer:
According to the available published data, the inhibition of Etoposide is specific to TOP2A. But there're also two papers showing that Etoposide could inhibit the p34cdc2 Kinase Activity: 1. http://cancerres.aacrjournals.org/content/52/7/1817.short ; 2. http://cancerres.aacrjournals.org/content/50/12/3761.short.

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