Etoposide (VP-16)

For research use only.

Catalog No.S1225 Synonyms: VP-16213

190 publications

Etoposide (VP-16) Chemical Structure

CAS No. 33419-42-0

Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.

Selleck's Etoposide (VP-16) has been cited by 190 publications

Purity & Quality Control

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Biological Activity

Description Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.
Targets
Topo II [2]
(Cell-free assay)
In vitro

Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA, which induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle. [1] Etoposide inhibits the growth of murine angiosarcoma cell line (ISOS-1) in a 5 days-period with IC50 of 0.25 μg/mL. Cell growth of normal murine microvascular endothelial cells (mECs) is less sensitive to Etoposide with IC50 of 10 μg/mL). [2] Etoposide treated for 6 hr inhibits colonies of tetraploid variant of the human leukemic lymphoblast line CCRF-CEM with IC50 of 0.6 μM. [3] Etoposide treated for 2 hr inhibits growth of human pancreatic cancer cell line Y1, Y3, Y5, Y19, YM. YS, and YT with IC50s of 300 μg/mL, 300 μg/mL, 300 μg/mL, 91 μg/mL, 0.68 μg/mL, 300 μg/mL, 300 μg/mL, and 260 μg/mL, respectively. [4] Etoposide exposed for 1 hr inhibits growth of human glioma cell lines CL5, G142, G152, G111, and G5 with IC50 of 8, 9, 9.8, 10, and 15.8 μg/mL respectively for 12 days. Under same condition, the IC90 value is attained in cell lines CL5, G152, G142, and G111 at 26, 27, 32, and 33 μg/mL. Etoposide inhibition of topoisomerase II is homogeneous for each cell. The average inhibition rates are 15%, 21.8%, 31.8%, 41.5%, and 49.5% for 1, 2, 4, 8, and 16 μg Etoposide, respectively. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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K562 MWPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M4HMb|Q5KGi{cx?= MnHsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNNU[yJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkK3JO69VS5? NUjBcZl2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NVQ6PzJpPkG4OVE1QTd{PD;hQi=>
KB MnvWRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NHPjeGM1QCCqcoO= Mo[xRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNQjDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC52MjFOwG0v MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDVzNEm3Nkc,OTh3MUS5O|I9N2F-
L02 NEHoe2JCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NYe1UXp4PDhiaILz NFGwUIdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFywNkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMUOg{txONg>? M1LacFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUG0PVczLz5zOEWxOFk4OjxxYU6=
DU145 ATCC HTB 81 NGrxRYREgXSxdH;4bYNqfHliYYPzZZk> MlH4O|IhcHK| M4fqSGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IFHUR2MhUFSEIEixJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkWg{txONg>? MmjrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
K562 ATCC CCL 243 NIniXWREgXSxdH;4bYNqfHliYYPzZZk> MnPXO|IhcHK| MlzkR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiCDVFPDJGNEVCB{NEOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDRwOTFOwG0v NELEcGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW1PFQ6OCd-MUi1OVg1QTB:L3G+
MCF7 ATCC HTB 22 NVi3RpNZS3m2b4TvfIlkcXS7IHHzd4F6 M4fRcVczKGi{cx?= NX7PWnFnS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDBWGNEKEiWQjCyNkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMUKuNkDPxE1w MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV3OES5NEc,OTh3NUi0PVA9N2F-
U251 RCB 0641 NFXDR5ZEgXSxdH;4bYNqfHliYYPzZZk> NETLVHM4OiCqcoO= Mn7jR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVI2OSCUQ1KgNFY1OSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE42KM7:TT6= Mn7KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
3LL CRL 1642 NWGydYtJS3m2b4TvfIlkcXS7IHHzd4F6 MknVOFghcHK| NXfJfZcxS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhO0yOIFPSUEAyPjR{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUA1KM7:TT6= M4TC[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUW4OFkxLz5zOEW1PFQ6ODxxYU6=
L1210 ATCC CCL 219 NYfNNoc1S3m2b4TvfIlkcXS7IHHzd4F6 Mn:5OFghcHK| MnP4R5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUFEzOTBiQWTDR{BES0xiMkG5JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkWg{txONg>? MkjKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
SK-Hep1 MkjkR5l1d3SxeHnjbZR6KGG|c3H5 NInTR4xEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2KZYCxJINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFQ6NjZizszNMi=> NED6e4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[zO|Y5QCd-MUi2N|c3QDh:L3G+
HL60 MVrDfZRwfG:6aXPpeJkh[XO|YYm= MnXJR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{BjgSCVUlKgZZN{[XluIFnDOVAhRSByLkKg{txONg>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ3MUe3NUc,OTh4NUG3O|E9N2F-
A549 M2DI[GN6fG:2b4jpZ4l1gSCjc4PhfS=> NIKxRW9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDv[kBk\WyuIHfyc5d1cCCkeTDTVmIhdWW2aH;kMEBKSzVyIE2gPU46QCEQvF2u M1Lse|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{iyOlY5Lz5zOEe4NlY3QDxxYU6=
KB MY\DfZRwfG:6aXPpeJkh[XO|YYm= MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hd2ZiY3XscEBoem:5dHigZpkhW1KEIH3leIhw\CxiSVO1NEA:KDF2LkKg{txONg>? NFHqdGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEe4NlY3QCd-MUi3PFI3Pjh:L3G+
A549 M32wfmN6fG:2b4jpZ4l1gSCjc4PhfS=> MWq3NkBpenN? Mli0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gPE4{PyEQvF2u NXHmeYJwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4OFQ1OjJpPkG4PFQ1PDJ{PD;hQi=>
KB MWTDfZRwfG:6aXPpeJkh[XO|YYm= NYHVPVFLPzJiaILz M2nodWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCxMlI2KM7:TT6= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh2NESyNkc,OTh6NES0NlI9N2F-
AGS NVHrcXJuS3m2b4TvfIlkcXS7IHHzd4F6 MVKzJIRigXN? M{TvXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGFIWyClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4{PiEQvF2u M1jEflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEGzNFc1Lz5zOUCxN|A4PDxxYU6=
HL-60 MlPhR5l1d3SxeHnjbZR6KGG|c3H5 NUDhVnhYOyCmYYnz NEH4TGVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVC14MDDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC56IN88UU4> MnuxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyMUOwO|QoRjF7MEGzNFc1RC:jPh?=
J82 MYjDfZRwfG:6aXPpeJkh[XO|YYm= NXPqWYdZOyCmYYnz MlrRR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTlgzKGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCyMlgh|ryPLh?= M2ruOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEGzNFc1Lz5zOUCxN|A4PDxxYU6=
MRC5 MnTBR5l1d3SxeHnjbZR6KGG|c3H5 NH:3c2s{KGSjeYO= M{PaSWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1TSzViY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFMvQSEQvF2u M3fBV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEGzNFc1Lz5zOUCxN|A4PDxxYU6=
SK-MES-1 NHn1RYhEgXSxdH;4bYNqfHliYYPzZZk> NEnsN3I{KGSjeYO= NUP4eoJbS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXTMVEh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3OjeTygTWM2OCB;IEKuOUDPxE1w NYfrPHJDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwNVMxPzRpPkG5NFE{ODd2PD;hQi=>
A375 MXHDfZRwfG:6aXPpeJkh[XO|YYm= NIDlPY5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOzd3IHPlcIx{KGK7IF3UWEBie3OjeTygTWM2OCB;IEOuPVIh|ryPLh?= NGK2NpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCzOlU5PCd-MUmwN|Y2QDR:L3G+
A549 MWLDfZRwfG:6aXPpeJkh[XO|YYm= NVj0TZMzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC5MlUyKM7:TT6= NGTHb3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCzOlU5PCd-MUmwN|Y2QDR:L3G+
HL60 MYnDfZRwfG:6aXPpeJkh[XO|YYm= M4\0TmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT64N{DPxE1w MmfmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyM{[1PFQoRjF7MEO2OVg1RC:jPh?=
THP1 MW\DfZRwfG:6aXPpeJkh[XO|YYm= NWH5bFVHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXEiSMTDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCyMlE3KM7:TT6= NYK1fVlvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwN|Y2QDRpPkG5NFM3PTh2PD;hQi=>
A549 NVnOc5h3S3m2b4TvfIlkcXS7IHHzd4F6 NXu1bJN7PzJiaILz MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC1Mlkh|ryPLh?= NFP5Tok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC1N|UyPCd-MUmwOVM2OTR:L3G+
HL-60 MlPjR5l1d3SxeHnjbZR6KGG|c3H5 M1ziTVczKGi{cx?= NIqzT4FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVC14MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5{NTFOwG0v MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{WxOEc,OTlyNUO1NVQ9N2F-
U937 NW\SdnpWS3m2b4TvfIlkcXS7IHHzd4F6 NGrJfGY4OiCqcoO= NGnJU2NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWQTN5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjl{IN88UU4> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{WxOEc,OTlyNUO1NVQ9N2F-
ACHN NYrDbVJOSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NWO5XFViQTZiaILz NG\Bc3FCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFHDTG4h[2WubIOgZYZ1\XJiOU[gbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEKuNVUh|ryPLh?= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2O{c,OTlyNUO3Olc9N2F-
CCRF-CEM NWi2SYdHSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NHXwelI6PiCqcoO= NU[5XpVKSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDR3JHNUOHTTDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOS5{IN88UU4> MlrlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyNUO3OlcoRjF7MEWzO|Y4RC:jPh?=
CCRF-SB M1vUcWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUO5OkBpenN? NWfC[5pTSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDR3JHNVOEIHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjFizszNMi=> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2O{c,OTlyNUO3Olc9N2F-
HeLa NXfsW2ZqSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NHfpe2o6PiCqcoO= M3T1bmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXMZUBk\WyuczDh[pRmeiB7NjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMzFOwG0v MlzuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyNUO3OlcoRjF7MEWzO|Y4RC:jPh?=
HT-29 NX\0RYVtSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MYe5OkBpenN? M3rPTmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiOU[gbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEKuOUDPxE1w M17FT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzO|Y4Lz5zOUC1N|c3PzxxYU6=
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MCF7 NYKwPZl7S3m2b4TvfIlkcXS7IHHzd4F6 MVK0PEBpenN? M4L0WWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvPDhizszNMi=> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV4NEO4N{c,OjJ3NkSzPFM9N2F-
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COLO205 NVjqRVYzSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= Ml7hOFghcHK| NWPjOXRMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDU2xQOjB3IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjR|IN88UU4> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV5OES1Nkc,OjJ3N{i0OVI9N2F-
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A549 MmXvR5l1d3SxeHnjbZR6KGG|c3H5 NF;kXYE4OiCqcoO= NFixOHZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUA6NjNizszNMi=> Mle2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2MEK2N|IoRjJ3NECyOlMzRC:jPh?=
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ZR-7530 M4[zUGN6fG:2b4jpZ4l1gSCjc4PhfS=> MlTKO|IhcHK| MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDaVk04PTNyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzNjVizszNMi=> NV\oSpdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0NFI3OzJpPkK1OFAzPjN{PD;hQi=>
SKMES1 NY\x[FRFS3m2b4TvfIlkcXS7IHHzd4F6 NUexS3VqS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uPRWOxJINmdGy|LDDJR|UxKD1iMT64N{DPxE1w MlKwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NEC4PFQoRjJ3NESwPFg1RC:jPh?=
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MCF7 MWfDfZRwfG:6aXPpeJkh[XO|YYm= M4XLUlczKGi{cx?= M3;JeWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFI{NjlizszNMi=> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR3M{e5PUc,OjV2NUO3PVk9N2F-
ME180 MnuzR5l1d3SxeHnjbZR6KGG|c3H5 MmDUO|IhcHK| NHfvOnNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTTF6MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hQC57IN88UU4> M4\EVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEWzO|k6Lz5{NUS1N|c6QTxxYU6=
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A549 MnTOR5l1d3SxeHnjbZR6KGG|c3H5 M4jp[FQ5KGi{cx?= NUDZc5pzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOTBwMUKg{txONg>? M2HqbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NE[yNlM{Lz5{NUS2NlI{OzxxYU6=
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A549 NWHNSo12S3m2b4TvfIlkcXS7IHHzd4F6 M1LBWVQ5KGi{cx?= NHjPfmpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBCdGGvYYKgRox2\SCjc4PhfUwhUUN3MDC9JFIvQDdizszNMi=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4NkG4O{c,OjV2Nk[xPFc9N2F-
L6 MVfDfZRwfG:6aXPpeJkh[XO|YYm= MmHsO|EvPSCqcoO= NY[4V5NOS3m2b4TvfIlkcXS7IHHnZYlve3RicnH0JGw3KGOnbHzzJIFnfGW{IEexMlUhcHK|IHL5JHBz\XO2bzDCcJVmKGG|c3H5MEBKSzVyIE2gNVgvQTlizszNMi=> NFjQXoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2OlE5Pyd-MkW0OlYyQDd:L3G+
DU145 NV\OXGFyS3m2b4TvfIlkcXS7IHHzd4F6 M{jQdFIh\GG7cx?= NUnwXoVbS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTFVzNEWgZ4VtdHNiYX\0[ZIhOiCmYYnzJIJ6KEOFSz24JIF{e2G7LDDJR|UxKD1iMD61JO69VS5? NV\IfW5VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PFE{QTZpPkK1OFgyOzl4PD;hQi=>
HCT15 MX7DfZRwfG:6aXPpeJkh[XO|YYm= M3vFUFIh\GG7cx?= M{PLc2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDF3IHPlcIx{KGGodHXyJFIh\GG7czDifUBES0tvODDhd5NigSxiSVO1NEA:KDFwMTFOwG0v NU[weWY6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PFE{QTZpPkK1OFgyOzl4PD;hQi=>
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A549 MlT4R5l1d3SxeHnjbZR6KGG|c3H5 M4L5TFczKGi{cx?= MlfYR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiSVO1NEA:KDBwODFOwG0v NX;5eYl3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVU1OjJpPkK1OFk2PDJ{PD;hQi=>
HCT116 MVnDfZRwfG:6aXPpeJkh[XO|YYm= NUPo[pJSPzJiaILz MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFnDOVAhRSBzLkmg{txONg>? NWL3TYRXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVU1OjJpPkK1OFk2PDJ{PD;hQi=>
K562 M{DOVmN6fG:2b4jpZ4l1gSCjc4PhfS=> NYPUe4VkPzJiaILz M2DnNWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGs2PjJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFnDOVAhRSB|LkGg{txONg>? NYjHbYNERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVU1OjJpPkK1OFk2PDJ{PD;hQi=>
MDA-MB-231 NF\tW5BEgXSxdH;4bYNqfHliYYPzZZk> MorFO|IhcHK| M1XYeWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZktKEmFNUCgQUAyOC54IN88UU4> NWPtOII2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVU1OjJpPkK1OFk2PDJ{PD;hQi=>
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A549 MkL1RY51cWOjbnPldkBie3OjeR?= NX\HVYtYPDhiaILz NFvESldCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gN{4xOSEQvF2u M4jQfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkG1O|k3Lz5{NU[xOVc6PjxxYU6=
DU145 M2L2VmFvfGmlYX7j[ZIh[XO|YYm= MmryOFghcHK| NYnxTHdnSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAyNjl6IN88UU4> M{LReFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkG1O|k3Lz5{NU[xOVc6PjxxYU6=
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RAW264.7 MYrDfZRwfG:6aXPpeJkh[XO|YYm= MWG3NkBpenN? Ml7VR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IEeyJIhzeyCkeTDNWHQh[2:ub4LpcYV1emmlIHHzd4F6NCCLQ{WwJF0hOC56MjFOwG0v M4rKdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkS0OlczLz5{NU[0OFY4OjxxYU6=
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A549 NFnNRWJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NIWweJo{KGSjeYO= NIH5W3NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZktKEmFNUCgQUAxNjhizszNMi=> NIrNdWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUewNFI{Oid-MkW3NFAzOzJ:L3G+
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MDA-MB-231 NX3qdnZ[SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NIXJNlk{KGSjeYO= NXS5[VBFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDNiZHH5d{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5MEBKSzVyIE2gNVEh|ryPLh?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyMEKzNkc,OjV5MECyN|I9N2F-
SKHEP1 NEj5PG5CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MWSzJIRigXN? NH;2d5dCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLTGVROSClZXzsd{Bi\nSncjCzJIRigXNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiSVO1NEA:KDFwMTFOwG0v MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyMEKzNkc,OjV5MECyN|I9N2F-
SNU638 M3;1dmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MXqzJIRigXN? M{jubGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU17VOlM5KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwJF0hOC53IN88UU4> NXvMXI1{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NFAzOzJpPkK1O|AxOjN{PD;hQi=>
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SMMC7721 NInuUYpCdnSrY3HuZ4VzKGG|c3H5 MYK0PEBpenN? NVOwNJE6SW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJHNOVUN5N{KxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB6LkGyJO69VS5? Mn7KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NESxPVAoRjJ3N{S0NVkxRC:jPh?=
SW480 MnjVRY51cWOjbnPldkBie3OjeR?= MoTJOFghcHK| NFPWU2JCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gV3c1QDBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFE4NjFzIN88UU4> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd2NEG5NEc,OjV5NESxPVA9N2F-
HepG2 M{e5bmN6fG:2b4jpZ4l1gSCjc4PhfS=> NXPYdoNlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOFAvPDdizszNMi=> MnTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NEe0PVkoRjJ3N{S3OFk6RC:jPh?=
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CCRF-CEM MVrDfZRwfG:6aXPpeJkh[XO|YYm= NEP3Z5Q4OiCqcoO= NGnYe5dEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBES1KILVPFUUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDHTVUxKD1iMT62JO69VS5? NGLUbmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe5NlE1Oid-MkW3PVIyPDJ:L3G+
HT-29 NVzJT|ByS3m2b4TvfIlkcXS7IHHzd4F6 MmC1O|IhcHK| NXzOZlA6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFRvMkmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiR1m1NEA:KDNzLk[g{txONg>? NUn2OYVIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3PVIyPDJpPkK1O|kzOTR{PD;hQi=>
MCF7 NWHYd4hFS3m2b4TvfIlkcXS7IHHzd4F6 NV\IS4pMPzJiaILz Mm\VR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBIUTVyIE2gNVkvQSEQvF2u M{LDXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{myNVQzLz5{NUe5NlE1OjxxYU6=
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A549 M{X5[mN6fG:2b4jpZ4l1gSCjc4PhfS=> NGDURWo1QCCqcoO= MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCpcn;3eIghcW6ldXLheIVlKG[xcjC0PEBpenNiYomgTI9m[2i|dDDhd5NigSxiSVO1NEA:KDBwNEig{txONg>? NVLjZm1YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yNlU6ODVpPkK2NlI2QTB3PD;hQi=>
DLD1 MWTDfZRwfG:6aXPpeJkh[XO|YYm= MYO0PEBpenN? NW\ldlNGS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTEyGMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGOnbHyg[5Jwf3SqIHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KEixZXPod5Qh[XO|YYmsJGlEPTBiPTCzMlMh|ryPLh?= NX;yNpJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yNlU6ODVpPkK2NlI2QTB3PD;hQi=>
HepG2 Mk\FRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M4jDVlczKGi{cx?= MnrDRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZYDHNkBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iM{Cg{txONg>? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ7MUCzO{c,OjZ{OUGwN|c9N2F-
MDA-MB-231 NIL2SlZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NF7zN2E4OiCqcoO= MoLrRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFczKGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUA{NjZizszNMi=> M3Gz[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkmxNFM4Lz5{NkK5NVA{PzxxYU6=
MDA-MB-435 NUfDcZJuSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M4W4V|czKGi{cx?= NFi2OJRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi12M{WgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDFwNzFOwG0v MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ7MUCzO{c,OjZ{OUGwN|c9N2F-
PC3 M1vpOmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NILSblA4OiCqcoO= NHP3U3pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDDN{Bk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iMTFOwG0v NX;wO3J{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yPVExOzdpPkK2NlkyODN5PD;hQi=>
A549 MnrBR5l1d3SxeHnjbZR6KGG|c3H5 NEjlbm81QCCqcoO= MnrlR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gN|gvPSEQvF2u Mo\iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{OUK2NlgoRjJ4MkmyOlI5RC:jPh?=
DU145 M3\pe2N6fG:2b4jpZ4l1gSCjc4PhfS=> NHuwUG01QCCqcoO= NID3bXNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPS5{MTFOwG0v NIXjSFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK5NlYzQCd-Mk[yPVI3Ojh:L3G+
HGC Mn7KR5l1d3SxeHnjbZR6KGG|c3H5 NEjwd|I1QCCqcoO= NYm0TodYS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEeFIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAyOC57IN88UU4> Ml7aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{OUK2NlgoRjJ4MkmyOlI5RC:jPh?=
MDA-MB-231 MXHDfZRwfG:6aXPpeJkh[XO|YYm= MX60PEBpenN? MofOR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iNEmuN|Uh|ryPLh?= M123Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkmyOlI5Lz5{NkK5NlYzQDxxYU6=
RWPE1 Mkj4R5l1d3SxeHnjbZR6KGG|c3H5 M2jzZlQ5KGi{cx?= MWDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSW3BGOSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNlcvPjRizszNMi=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ7Mk[yPEc,OjZ{OUK2Nlg9N2F-
HCT15 M1P3PWN6fG:2b4jpZ4l1gSCjc4PhfS=> MVWyJIRigXN? Mon4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTViY3XscJMhdWWjc4Xy[YQh[W[2ZYKgNkBl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFUvPTRizszNMi=> NXjpXnZURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zN|Q1QTlpPkK2N|M1PDl7PD;hQi=>
HeLa M{K5eGN6fG:2b4jpZ4l1gSCjc4PhfS=> MXuyJIRigXN? NUjDOW5OS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCvZXHzeZJm\CCjZoTldkAzKGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPy54IN88UU4> Ml\iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|M{S0PVkoRjJ4M{O0OFk6RC:jPh?=
T47D NFfLZphEgXSxdH;4bYNqfHliYYPzZZk> NHzwdo4zKGSjeYO= NEPqZ5hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVPDeGIHPlcIx{KG2nYYP1doVlKGGodHXyJFIh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUA6NjJ6IN88UU4> NXjHUnNJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zN|Q1QTlpPkK2N|M1PDl7PD;hQi=>
GBM2 MX\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Ml\yO|IhcHK| NYDJRmJmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDHRm0zKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBYW1RvMTDt[ZRpd2RuIFnDOVAhRSBzLkGg{txONg>? NITDNZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO1OVU{Oid-Mk[zOVU2OzJ:L3G+
DU145 MW\DfZRwfG:6aXPpeJkh[XO|YYm= MlLVNkBl[Xm| MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjCyJIRigXNiYomgR2NMQCCjc4PhfUwhUUN3MDC9JFE{NjFizszNMi=> NWDxOWRqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zOlE4OzdpPkK2N|YyPzN5PD;hQi=>
HCT15 NYK3SI5OS3m2b4TvfIlkcXS7IHHzd4F6 MYmyJIRigXN? MkThR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTViY3XscJMh[W[2ZYKgNkBl[Xm|IHL5JGNEUzhiYYPzZZktKEmFNUCgQUAzNjZizszNMi=> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN4MUezO{c,OjZ|NkG3N|c9N2F-
MCF10A MYDDfZRwfG:6aXPpeJkh[XO|YYm= M3jKN|Ih\GG7cx?= NUnQdFZ6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOIMUDBJINmdGy|IHHmeIVzKDJiZHH5d{BjgSCFQ1u4JIF{e2G7LDDJR|UxKD1iMUCuNVQh|ryPLh?= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN4MUezO{c,OjZ|NkG3N|c9N2F-
T47D M33oZmN6fG:2b4jpZ4l1gSCjc4PhfS=> NHfOR3gzKGSjeYO= MkXNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWFQ4TCClZXzsd{Bi\nSncjCyJIRigXNiYomgR2NMQCCjc4PhfUwhUUN3MDC9JFAvQTRizszNMi=> M2XnTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{[xO|M4Lz5{NkO2NVc{PzxxYU6=
HepG2 NFGyRmREgXSxdH;4bYNqfHliYYPzZZk> M2\JcFQ5KGi{cx?= MULDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gN|MvQThizszNMi=> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{O5N{c,OjZ|OUezPVM9N2F-
MOLT3 MnHNR5l1d3SxeHnjbZR6KGG|c3H5 MXW0PEBpenN? NH;ndW1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOV0yWMzDj[YxteyCjZoTldkA1QCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwJF0hOC5yNEGg{txONg>? NGX6VHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|M6Oyd-Mk[zPVc{QTN:L3G+
A549 MoPIR5l1d3SxeHnjbZR6KGG|c3H5 NG\HWJA1QCCqcoO= NVXPS2x5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nlicnXzZZp2emmwIHHzd4F6NCCLQ{WwJF0hOS55IN88UU4> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7Mki5O{c,OjZ3OUK4PVc9N2F-
DLD1 NWPjUohKS3m2b4TvfIlkcXS7IHHzd4F6 M2jNOFQ5KGi{cx?= MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEUGQyKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFQ5KGi{czDifUBz\XOjeoXybY4h[XO|YYmsJGlEPTBiPTCyJO69VS5? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7Mki5O{c,OjZ3OUK4PVc9N2F-
A549 NFLocXdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NF7z[4EzPCC2bzC3NkBpenN? NIXjdFNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiMkSgeI8hPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDBwODFOwG0v MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7NUi3OUc,OjZ3OUW4O|U9N2F-
CAKI-1 NYi2TphpSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NHP0UnMzPCC2bzC3NkBpenN? NEPKSHRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFPBT2kuOSClZXzsd{Bi\nSncjCyOEB1dyB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iMT6xJO69VS5? Ml7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUW4O|UoRjJ4NUm1PFc2RC:jPh?=
HCT116 M2HFcGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M3vQfFI1KHSxIEeyJIhzew>? M{DZWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIFnfGW{IEK0JJRwKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSB|LkOg{txONg>? NUHqfJBKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVU5PzVpPkK2OVk2QDd3PD;hQi=>
HCT116 MmPGRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1qydVQ5KGi{cx?= MUjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iMj6zJO69VS5? NEeyOHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5OVg4PSd-Mk[1PVU5PzV:L3G+
HCT116 NWHjS4dUSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NUT4cYRMPzJiaILz NX[0RZZzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvOyEQvF2u NYrPWGdlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVU5PzVpPkK2OVk2QDd3PD;hQi=>
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HCT116 NF:0TGJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MmDOO|IhcHK| MYPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IIC1N{1mgHC{ZYPzbY5oKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOS5zIN88UU4> NXvhXYVlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVU5PzVpPkK2OVk2QDd3PD;hQi=>
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DU145 NGT1[ItCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MUi3NkBpenN? NFfNS3NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTVNVQ2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCxJO69VS5? NY\CUG5VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwO|c1ODRpPkK5NFc4PDB2PD;hQi=>
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WI38 NF\GbYlEgXSxdH;4bYNqfHliYYPzZZk> M2jzRlQ5KGi{cx?= NIe4SY1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBYUTN6IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEK1Mlch|ryPLh?= M4CwdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MkS4Nlk3Lz5{OUK0PFI6PjxxYU6=
A549 Mo\OR5l1d3SxeHnjbZR6KGG|c3H5 M176d|Q5KGi{cx?= Mn3zR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNk4xOyEQvF2u NFPwPVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUK2PVI2Oyd-MkmyOlkzPTN:L3G+
DU145 NFTxdVhEgXSxdH;4bYNqfHliYYPzZZk> NH7YT5g1QCCqcoO= MnPyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSHUyPDViY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFIvODNizszNMi=> M2HJPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7Mk[5NlU{Lz5{OUK2PVI2OzxxYU6=
HeLa M4DZNGN6fG:2b4jpZ4l1gSCjc4PhfS=> NITHPGg1QCCqcoO= MorCR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNk4xOyEQvF2u NEjoXVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUK2PVI2Oyd-MkmyOlkzPTN:L3G+
MCF7 MWXDfZRwfG:6aXPpeJkh[XO|YYm= M3LqeVQ5KGi{cx?= MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCyMlA{KM7:TT6= NWK3c3hJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyOlkzPTNpPkK5NlY6OjV|PD;hQi=>
A549 MlO5R5l1d3SxeHnjbZR6KGG|c3H5 M1rB[FQ5KGi{cx?= NUj0PFBLS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOi5{NzFOwG0v NXvEeZdYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyPFk5QDFpPkK5Nlg6QDhzPD;hQi=>
DU145 MYPDfZRwfG:6aXPpeJkh[XO|YYm= MXy0PEBpenN? MkXjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSHUyPDViY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFEvQTdizszNMi=> M3nKOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7Mki5PFgyLz5{OUK4PVg5OTxxYU6=
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HT-29 NGPVXnlEgXSxdH;4bYNqfHliYYPzZZk> NGrhXW01QCCqcoO= M1q3[GN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAyNjZ2IN88UU4> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTJ6OUi4NUc,Ojl{OEm4PFE9N2F-
MDA-MB-231 M{DGW2N6fG:2b4jpZ4l1gSCjc4PhfS=> MlnMOFghcHK| NU\NNJhGS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNU46OSEQvF2u MofHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{OEm4PFEoRjJ7Mki5PFgyRC:jPh?=
A549 M{fKfWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NIf3T2o1QCCqcoO= NUHE[28xSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCxMlk4KM7:TT6= NEH6eW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUK4PVg5PCd-MkmyPFk5QDR:L3G+
DU145 MonwRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NEG5blY1QCCqcoO= Mor1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCGVUG0OUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj6zOUDPxE1w MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTJ6OUi4OEc,Ojl{OEm4PFQ9N2F-
HeLa MUjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnfrOFghcHK| Mk\NRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB{LkexJO69VS5? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTJ6OUi4OEc,Ojl{OEm4PFQ9N2F-
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NCI-H1618 NWfxRYpyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWXJR|UxKD1iNECuNVA1OSEQvF2= Moi0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
MFE-280 MlHjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1myc2lEPTBiPTC0NE42ODB3IN88US=> M1m4RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1PjV5Lze+V2FPT0WUPD;hQi=>
EB-3 NYewR4ZqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnvITWM2OCB;IESwMlYzPDNizszN NV;Fd3dxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR4NUevK|5USU6JRWK8M4E,
IM-9 NX61NVRVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MljDTWM2OCB;IESxMlY{PDFizszN M17ydlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1PjV5Lze+V2FPT0WUPD;hQi=>
DV-90 NIT3V2RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{nOSmlEPTBiPTC0NU46PzRzIN88US=> MlPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
NCI-H1792 MmnwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVXJR|UxKD1iNEKuOlg2QCEQvF2= NYfVfnNGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR4NUevK|5USU6JRWK8M4E,
SNU-387 MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4nJU2lEPTBiPTC0N{4zOThzIN88US=> NF[xN3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDZ3Nz:nQnNCVkeHUkyvZV4>
HCC1395 MnfjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVvJR|UxKD1iNESuO|k2OyEQvF2= M{ftXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1PjV5Lze+V2FPT0WUPD;hQi=>
TGW NIjodHJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGWxe4NKSzVyIE2gOFYvOzJ6OTFOwG0> MknEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
KY821 MlvpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mn\mTWM2OCB;IES3MlA6OzJizszN NYfIN5FbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR4NUevK|5USU6JRWK8M4E,
ECC4 MkTnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXrJR|UxKD1iNEiuNFE5OiEQvF2= NW\5ZmxURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR4NUevK|5USU6JRWK8M4E,
KARPAS-45 MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEjwS3JKSzVyIE2gOFgvOjN3OTFOwG0> NIrXd|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDZ3Nz:nQnNCVkeHUkyvZV4>
LB2518-MEL Mke2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2O3UmlEPTBiPTC0PE42ODV4IN88US=> M3fEelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1PjV5Lze+V2FPT0WUPD;hQi=>
COR-L88 MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3TPSmlEPTBiPTC0PE44OzVizszN Mn7oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
COLO-741 NGPaZotIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M375cmlEPTBiPTC0PE45QTZ4IN88US=> NYS3eVZWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR4NUevK|5USU6JRWK8M4E,
HCE-T MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3mxXWlEPTBiPTC0PU4{ODFizszN NFrBboM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDZ3Nz:nQnNCVkeHUkyvZV4>
NCI-H1755 M4nST2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVHJR|UxKD1iNEmuN|Q3QCEQvF2= Mo\3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p53 / p-p53 / Acetyl-p53 / TF-IIB; 

PubMed: 23800173     


Western blotting analysis of p53 levels and the indicated p53 post-translational modifications in the nucleus and cytoplasm of U-2 OS cells treated with no drug, 1 μmol/l etoposide (treated for 24 hours) and 100 μmol/l etoposide (treated for 12 hours), re䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ뙠ෆ䐺痖暼瘿뙠ෆᾰƌ 뙠ෆÐ㺣

p53 / p21 / c-Myc / PARP ; 

PubMed: 20212154     


Myc is repressed at the posttranscriptional level following etoposide treatment. HEK293 cells were treated with etoposide at the indicated concentrations for 24 h. Parallel samples were taken for western and northern analysis as indicated. 

23800173 20212154
Immunofluorescence
COX IV; 

PubMed: 29221178     


Panel 1 (untreated) depicts the mitochondrial distribution by visualizing the expression of Cox IV in U87(WT) cells. Panel 2 (etoposide treated) represents the distribution of mitochondria in U87(RETO) cells after 10 days of drug exposure as visualized by䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ

γH2AX / pATM ; 

PubMed: 19858003     


HeLa cells were treated with vehicle (Ctl), 6 Gy IR (IR) or 25 μM etoposide (ETO). Co-localization (yellow signal in the overlay (OL)) of gH2AX (red) and pATM (green). Scale bar = 20 μm.

MDC1; 

PubMed: 19858003     


HeLa cells were treated with vehicle (Ctl), 6 Gy IR (IR) or 25 μM etoposide (ETO). Co-localization of gH2AX with MDC1.

pDNA-PKcs; 

PubMed: 19858003     


HeLa cells were treated with vehicle (Ctl), 6 Gy IR (IR) or 25 μM etoposide (ETO). Co-localization of gH2AX with pDNA-PK.

p53; 

PubMed: 27515249     


p53 localization in untreated and 12-h etoposide treated CD41+ MK cells. Cells were stained with anti-p53 antibody. TOTO-3 was used to stain the nucleus. Scale bar=20 μm.

YAP; 

PubMed: 27515249     


(D) YAP localization in untreated CD41+MK cells and CD41+ MK cells treated by 10 μM etoposide for five hours on day 10 of culture. Cells were stained with anti-YAP antibody. DAPI was used to stain the nucleus. Cells (150) were counted and categorized acco䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ삨Ղ䐺痖暼瘿삨Ղᾰƌ 삨ՂÐ㺣痖삨Ղ€𢡄사Ղ€䀷痗사Ղ౴사Ղ㵶痗사Ղ뺖᎒泌Itemセ᎒Count﫨呂샜Ղ

29221178 19858003 27515249
Growth inhibition assay
Cell viability ; 

PubMed: 22615609     


Effect of etoposide on HEK293 cell viability. Cells were seeded at 104 in 96-well plates and treated with etoposide (50,100,150 and 200µM) for 24 hrs and compared to nontreated cells (control). At the end of treatment period, cell viability was measured u䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒

22615609
In vivo Etoposide administrated as a single agent is found to been ineffective in many xenografts growth, such as Heterotransplanted Hepatoblastoma NMHB1, and NMHB 2, [6] human neuroblastoma xenograft, [7] and human gastrointestinal cancer xenograft, [8] while the dose of 10 mg/kg i.p. Etoposide inhibits murine angiosarcoma cell ISOS-1 tumors in 36% of controls. [2] Etoposide induces tumor immunity in Lewis lung cancer. A single administration of 50 mg/kg Etoposide i.p., induces a 60% survival of C57B1/6 mice injected with Lewis lung cancer cell (3LL) over 60 days. About 40% of these surviving mice reject a subsequent challenge with 3LL, while none of control mice survive beyond 30 days. 3LL cells which have survived an 90% lethal concentration of Etoposide in vitro kill 75% of recipient mice, but 60% surviving mice reject challenge with 3LL. Splenocytes harvested from tumor rejecting mice protect naive mice injected with 3LL. [9]

Protocol

Kinase Assay:[5]
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Topoisomerase II activity assay:

Nuclear extracts are prepared, and nuclei are isolated. The activity of topoisomerase II is calculated from the percentage of decatenation obtained. Tritiated kinoplast DNA (KDNA 0.22 μg) is used as a substrate. Etoposide and topoisomerase II are incubated for 30 min at 37 ℃ and are stopped with 1% sodium dodecyl sulfate (SDS) and proteinase K (100 μg/mL). The percentages of decatenation and inhibition of topoisomerase II by Etoposide are obtained.
Cell Research:[5]
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  • Cell lines: Human glioma cell lines CL5
  • Concentrations: 80 μg/mL
  • Incubation Time: 1 hour
  • Method: After the Etoposide treatment, cells are removed from the dish with phosphate-buffered saline (PBS) containing 0.03% trypsin and 0.27 mM ethylenediaminetetraacetic acid (EDTA) and are diluted into culture dishes in appropriate numbers to yield between 20 and 200 colonies. After 12 days, cultures are fixed with methanol-acetic acid, stained with crystal violet, and scored for colonies containing more than 50 cells. The standard errors are typically less than 15% of the mean value unless otherwise stated.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Murine angiosarcoma xenografts ISOS-1
  • Dosages: 10 mg/kg
  • Administration: i.p. every day for 5 days from day 7
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (169.9 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
15mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 588.56
Formula

C29H32O13

CAS No. 33419-42-0
Storage powder
in solvent
Synonyms VP-16213
Smiles CC1OCC2C(O1)C(C(C(O2)OC3C4COC(=O)C4C(C5=CC6=C(C=C35)OCO6)C7=CC(=C(C(=C7)OC)O)OC)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04935255 Not yet recruiting Other: Blood sampling - Pharmacokinetic assessment Testicular Cancer Radboud University August 1 2021 --
NCT04804007 Recruiting Drug: Etoposide Germ Cell Tumor|Non-seminomatous Germ Cell Tumor|Ovarian Germ Cell Tumor Nabil Adra|Indiana University March 3 2021 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    Regarding the Etoposide S1225, do you have any data of the inhibition sepcificity of this product? It will inhibit other enzymes other than TOP2A?

  • Answer:

    According to the available published data, the inhibition of Etoposide is specific to TOP2A. But there're also two papers showing that Etoposide could inhibit the p34cdc2 Kinase Activity: 1. http://cancerres.aacrjournals.org/content/52/7/1817.short ; 2. http://cancerres.aacrjournals.org/content/50/12/3761.short.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID