Etoposide

For research use only.

Catalog No.S1225 Synonyms: VP-16, VP-16213

170 publications

Etoposide Chemical Structure

CAS No. 33419-42-0

Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.

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Selleck's Etoposide has been cited by 170 publications

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Biological Activity

Description Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.
Targets
Topo II [2]
(Cell-free assay)
In vitro

Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA, which induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle. [1] Etoposide inhibits the growth of murine angiosarcoma cell line (ISOS-1) in a 5 days-period with IC50 of 0.25 μg/mL. Cell growth of normal murine microvascular endothelial cells (mECs) is less sensitive to Etoposide with IC50 of 10 μg/mL). [2] Etoposide treated for 6 hr inhibits colonies of tetraploid variant of the human leukemic lymphoblast line CCRF-CEM with IC50 of 0.6 μM. [3] Etoposide treated for 2 hr inhibits growth of human pancreatic cancer cell line Y1, Y3, Y5, Y19, YM. YS, and YT with IC50s of 300 μg/mL, 300 μg/mL, 300 μg/mL, 91 μg/mL, 0.68 μg/mL, 300 μg/mL, 300 μg/mL, and 260 μg/mL, respectively. [4] Etoposide exposed for 1 hr inhibits growth of human glioma cell lines CL5, G142, G152, G111, and G5 with IC50 of 8, 9, 9.8, 10, and 15.8 μg/mL respectively for 12 days. Under same condition, the IC90 value is attained in cell lines CL5, G152, G142, and G111 at 26, 27, 32, and 33 μg/mL. Etoposide inhibition of topoisomerase II is homogeneous for each cell. The average inhibition rates are 15%, 21.8%, 31.8%, 41.5%, and 49.5% for 1, 2, 4, 8, and 16 μg Etoposide, respectively. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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HT1080 M4Hyc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYqxMVExOCEQvF2= MknhOE8zPC92ODDo NFTQW4pFVVORwrC= Mkm4bY5lfWOnczDj[YxtKGSnYYToJJNq\26rZnnjZY51dHliaX6gZUB3\XK7IHzve{Bkd26lZX70doF1cW:w MkLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyN{iwOlQoRjJ3MEe4NFY1RC:jPh?=
HT1080 NVW1V3J[TnWwY4Tpc44hSXO|YYm= NIjER4QxNjByMEGtNVAxKM7:TR?= NIr3U2cyNTJ2IHi= M1rDU2ROW00EoB?= M{P1e4lv\HWlZYOgdE1xPTNqc3XyNVUqKGmwIHLveIghfGmvZT2gZY5lKGOxbnPlcpRz[XSrb36t[IVx\W6mZX70JI1idm6nch?= NF;2cnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC3PFA3PCd-MkWwO|gxPjR:L3G+
HT1080 MnP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWCwMlAxODFvMUCwJO69VQ>? M4rGOVI1KGh? MYTEUXNQyqB? NH3UeIlk[XW|ZYOgZY4hcW6lcnXhd4UhcW5idHjlJI52dWKncjDv[kBk\WyuczDpckBIOi:PLDD3bIlt\SCmZXPy[YF{cW6pIGOgZY5lKEdzIIDoZZNmKGOnbHzz MlHNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyN{iwOlQoRjJ3MEe4NFY1RC:jPh?=
HD-MY-Z NHrxRZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDrNlQwPDhxN{KgbC=> MVzJR|Ux97zgMUCwJO69VQ>? NX;P[Zp{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOFgzOzZpPkK1NFQ5OjN4PD;hQi=>
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DOHH-2 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHZO|IhcA>? MV;JR|UxRTYEoN88US=> NVPlfZlZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOFgzOzZpPkK1NFQ5OjN4PD;hQi=>
REH Mn7DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVWyOEBp NI[wdlZKSzVyPUCuNFI4yqEQvF2= M1XEPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MES4NlM3Lz5{NUC0PFI{PjxxYU6=
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HuT-78 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7jNlQhcA>? MXXJR|UxRTlwM9Mg{txO MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB2OEKzOkc,OjVyNEiyN|Y9N2F-
HuT-78 Mn7DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWW0PEBp M2jndmlEPTB;ND6zxsDPxE1? M2[5b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MES4NlM3Lz5{NUC0PFI{PjxxYU6=
HuT-78 NGTaUWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4[2XlczKGh? NEPEfmRKSzVyPUSuNuKh|ryP M1zEUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MES4NlM3Lz5{NUC0PFI{PjxxYU6=
OPM-2 Mle3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfCNZEzPCCq MXHJR|UxRTJ2LkJCpO69VQ>? Mo\vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNEiyN|YoRjJ3MES4NlM3RC:jPh?=
OPM-2 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYC0PEBp Mnf0TWM2OD12wrFOwG0> NVXMW257RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOFgzOzZpPkK1NFQ5OjN4PD;hQi=>
OPM-2 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjMcXE4OiCq MmCyTWM2OD1zLkRCpO69VQ>? MoWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNEiyN|YoRjJ3MES4NlM3RC:jPh?=
RPMI-8226 NEK1SXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrENlQhcA>? Mlz6TWM2OD1zME[uOuKh|ryP NXHiOY1JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOFgzOzZpPkK1NFQ5OjN4PD;hQi=>
RPMI-8226 NGLWUFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYK0PEBp NGm3VIJKSzVyPUmxMlHDqM7:TR?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB2OEKzOkc,OjVyNEiyN|Y9N2F-
RPMI-8226 M{jKdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\1O|IhcA>? M2nLOGlEPTB;MUSuPeKh|ryP MkfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNEiyN|YoRjJ3MES4NlM3RC:jPh?=
U-266 NYXaRZM{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWOxO|k6OjRiaB?= NW\BOZR1UUN3ME24Ok4zyqEQvF2= NXH0V|dSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOFgzOzZpPkK1NFQ5OjN4PD;hQi=>
U-266 NF7UWZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF[xWJU1QCCq NWXETXlQUUN3ME22PE41yqEQvF2= M4P5dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MES4NlM3Lz5{NUC0PFI{PjxxYU6=
U-266 MnT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXu3NkBp M3vTcmlEPTB;MkeuOOKh|ryP NX[3ephWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOFgzOzZpPkK1NFQ5OjN4PD;hQi=>
Kelly MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLTNE0yOCEQvF2= NHviPHU4OsLiaB?= M4jTW2lEPTB;MT61NVjDqM7:TR?= NFXyfpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCwPFkxOCd-MkWwNFg6ODB:L3G+
SH-SY5Y  NHfTSlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoO2NE0yOCEQvF2= MoCyO|LDqGh? MUHJR|UxRTBwN{W0xsDPxE4EoB?= NFLhNFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCwPFkxOCd-MkWwNFg6ODB:L3G+
SK-N-AS M2HX[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfZb2xHOC1zMDFOwG0> MmjUO|LDqGh? MYPJR|UxRTFwN{GyxsDPxE4EoB?= NWG4cplURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNFg6ODBpPkK1NFA5QTByPD;hQi=>
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HepG2 Ml\RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljDOFjDqGh? MXXEUXNQyqB? NWXmfos4UUN3ME2xN{43PcLiwsJCpFAvQTMEoN88US=> NXOycGhMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5PVYyOzZpPkK0PVk3OTN4PD;hQi=>
A549 M{DBVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfsOFjDqGh? MXTEUXNQyqB? NYP0T2RKUUN3ME2yOFEvQcLiwsJCpFMyNjJ|wrFOwG0> NX7kUWVkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5PVYyOzZpPkK0PVk3OTN4PD;hQi=>
MCF7 M13l[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIfZ[5U1QMLiaB?= M{fHcWROW00EoB?= NI[ydFBKSzVyPUixMlA6yqEEsdMgNVQvOjIEoN88US=> NXLDOlBjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5PVYyOzZpPkK0PVk3OTN4PD;hQi=>
HL-60  MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIn1OGo4OsLiaB?= MVfJR|UxRTBwMUNihKXPxE1? Mn\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OUOwNVQoRjJ2OUmzNFE1RC:jPh?=
HL-60[R] MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LuNFczyqCq M3fI[2lEPTB;Mz6xNwKBjc7:TR?= NETufGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5N|AyPCd-MkS5PVMxOTR:L3G+
MIAPACA NIX6RWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDRdmE1T0l3ME2xMlMhyrFiMD6wN{DPxE1? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl3M{iyNUc,OjR7NUO4NlE9N2F-
MCF-7 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHPS2k2OD1yLkK1JOKyKDBwMTFOwG0> NGf3Woc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm1N|gzOSd-MkS5OVM5OjF:L3G+
HeLa NV\xRllIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vGXGdKPTB;MD62OEDDuSByLkSg{txO NGLyTo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm1N|gzOSd-MkS5OVM5OjF:L3G+
MO59K  MlP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITa[Ig4KGR? M2LuVmlEPTB;MD6xO-KBjc7:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl3M{W2NUc,OjR7NUO1OlE9N2F-
MO59J NXvMNHBnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXFNG9xPyCm NG\SSINKSzVyPUCuNgKBjc7:TR?= MnTGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NUO1OlEoRjJ2OUWzOVYyRC:jPh?=
ME 180 MmPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfFXmRyPDkEoHlCpC=> MoH4TWM2OD16LkpCpOKyyqByLkRihKXPxE1? NUn6SIxvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OVMxOjdpPkK0PVU{ODJ5PD;hQi=>
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HL60 Mm\kR5l1d3SxeHnjbZR6KGG|c3H5 MYC3NkBpenN? MofuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= Mlf0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3MEe0O|IoRjF6NUC3OFczRC:jPh?=
HL60 M17CUWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MX60PEBpenN? NIXud4NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjMOlAh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuN|E2KM7:TT6= M1[0dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGzPVc1Lz5zOEWxN|k4PDxxYU6=
K562 M4DWfWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M1q4W|Q5KGi{cx?= M2fFbmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iS{W2NkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT6yO{DPxE1w M{DsflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUG0PVczLz5zOEWxOFk4OjxxYU6=
KB MUnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NX3UN2NrPDhiaILz MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEuEIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjR{IN88UU4> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDVzNEm3Nkc,OTh3MUS5O|I9N2F-
L02 NWTPSYtXSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NFLSXGQ1QCCqcoO= MnjSRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOMEKgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDF|IN88UU4> M{ftd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUG0PVczLz5zOEWxOFk4OjxxYU6=
DU145 ATCC HTB 81 NX7KVYVuS3m2b4TvfIlkcXS7IHHzd4F6 MlXoO|IhcHK| M2fYRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IFHUR2MhUFSEIEixJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkWg{txONg>? Mk\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
K562 ATCC CCL 243 NFrGb4pEgXSxdH;4bYNqfHliYYPzZZk> M4[xZ|czKGi{cx?= MYXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLOVYzKEGWQ1OgR2NNKDJ2MzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPC57IN88UU4> NIr6OFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW1PFQ6OCd-MUi1OVg1QTB:L3G+
MCF7 ATCC HTB 22 NYOxT2F{S3m2b4TvfIlkcXS7IHHzd4F6 M{Sxc|czKGi{cx?= NYnPPFdHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDBWGNEKEiWQjCyNkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMUKuNkDPxE1w MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV3OES5NEc,OTh3NUi0PVA9N2F-
U251 RCB 0641 MlK1R5l1d3SxeHnjbZR6KGG|c3H5 MYK3NkBpenN? MmfNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVI2OSCUQ1KgNFY1OSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE42KM7:TT6= MlTmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
3LL CRL 1642 NV;jTmpwS3m2b4TvfIlkcXS7IHHzd4F6 NVHWNWdWPDhiaILz M1K2[2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJFNNVCCFUlygNVY1OiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOEDPxE1w M3PaZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUW4OFkxLz5zOEW1PFQ6ODxxYU6=
L1210 ATCC CCL 219 MUnDfZRwfG:6aXPpeJkh[XO|YYm= NGPTXmE1QCCqcoO= NWX4Nnh4S3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhVDF{MUCgRXRESyCFQ1ygNlE6KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlUh|ryPLh?= MkP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
SK-Hep1 MVfDfZRwfG:6aXPpeJkh[XO|YYm= NGTLTm5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2KZYCxJINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFQ6NjZizszNMi=> M3rseVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkO3Olg5Lz5zOE[zO|Y5QDxxYU6=
HL60 M3HlTmN6fG:2b4jpZ4l1gSCjc4PhfS=> MonOR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{BjgSCVUlKgZZN{[XluIFnDOVAhRSByLkKg{txONg>? MnqyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NUG3O|EoRjF6NkWxO|cyRC:jPh?=
A549 NFPBSnJEgXSxdH;4bYNqfHliYYPzZZk> MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCxZjDj[YxtKGe{b4f0bEBjgSCVUlKgcYV1cG:mLDDJR|UxKD1iOT65PEDPxE1w MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDd6Mk[2PEc,OTh5OEK2Olg9N2F-
KB NWTqWW9IS3m2b4TvfIlkcXS7IHHzd4F6 NV\udnF7S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxiZ4Lve5RpKGK7IGPSRkBu\XSqb3SsJGlEPTBiPTCxOE4zKM7:TT6= M{f5TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{iyOlY5Lz5zOEe4NlY3QDxxYU6=
A549 NIfqb2FEgXSxdH;4bYNqfHliYYPzZZk> NH7KdYQ4OiCqcoO= MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC4MlM4KM7:TT6= NHLTV2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEi0OFQzOid-MUi4OFQ1OjJ:L3G+
KB MkfmR5l1d3SxeHnjbZR6KGG|c3H5 NH\HNoE4OiCqcoO= NY\RTFRqS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFEvOjVizszNMi=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh2NESyNkc,OTh6NES0NlI9N2F-
AGS MkHmR5l1d3SxeHnjbZR6KGG|c3H5 MnHmN{Bl[Xm| NU\xXlVOS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSUeVIHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjN4IN88UU4> NFnDR4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCxN|A4PCd-MUmwNVMxPzR:L3G+
HL-60 MnXuR5l1d3SxeHnjbZR6KGG|c3H5 NFfa[Gw{KGSjeYO= MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUE03OCClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE45KM7:TT6= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTBzM{C3OEc,OTlyMUOwO|Q9N2F-
J82 NXLheIhJS3m2b4TvfIlkcXS7IHHzd4F6 NV7ybZpqOyCmYYnz M4izZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGo5OiClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNk45KM7:TT6= M1\qWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEGzNFc1Lz5zOUCxN|A4PDxxYU6=
MRC5 NEPFdJNEgXSxdH;4bYNqfHliYYPzZZk> NGfOUFg{KGSjeYO= M4O1RmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1TSzViY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFMvQSEQvF2u MmjhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyMUOwO|QoRjF7MEGzNFc1RC:jPh?=
SK-MES-1 MUjDfZRwfG:6aXPpeJkh[XO|YYm= NU\1V5piOyCmYYnz NEPCcYZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2PRWOtNUBk\WyuczDh[pRmeiB|IHThfZMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj61JO69VS5? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTBzM{C3OEc,OTlyMUOwO|Q9N2F-
A375 Mki0R5l1d3SxeHnjbZR6KGG|c3H5 M2HXSGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE{PzViY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMz65NkDPxE1w NV62eoRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwN|Y2QDRpPkG5NFM3PTh2PD;hQi=>
A549 NUTr[lhuS3m2b4TvfIlkcXS7IHHzd4F6 MkG5R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB7LkWxJO69VS5? NF;rOGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCzOlU5PCd-MUmwN|Y2QDR:L3G+
HL60 MYPDfZRwfG:6aXPpeJkh[XO|YYm= MmfUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkizJO69VS5? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB|NkW4OEc,OTlyM{[1PFQ9N2F-
THP1 NGjZfZhEgXSxdH;4bYNqfHliYYPzZZk> M2jPPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHRJWDFiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj6xOkDPxE1w NIK4OIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCzOlU5PCd-MUmwN|Y2QDR:L3G+
A549 NH\qPW5EgXSxdH;4bYNqfHliYYPzZZk> M2rielczKGi{cx?= MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC1Mlkh|ryPLh?= NX3BN2ZPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwOVM2OTRpPkG5NFU{PTF2PD;hQi=>
HL-60 NHna[FJEgXSxdH;4bYNqfHliYYPzZZk> MlXwO|IhcHK| MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUE03OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4zPSEQvF2u NFTRRYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC1N|UyPCd-MUmwOVM2OTR:L3G+
U937 Ml7nR5l1d3SxeHnjbZR6KGG|c3H5 NVH4V5Y1PzJiaILz NYjaNm5bS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTl|NzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC57MjFOwG0v M1fF[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzOVE1Lz5zOUC1N|UyPDxxYU6=
ACHN MmTqRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M2jLSVk3KGi{cx?= MnH4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDQ1jOJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB{LkG1JO69VS5? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2O{c,OTlyNUO3Olc9N2F-
CCRF-CEM NHHtSGtCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MYG5OkBpenN? M{LOZ2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ1PSSk1ETU1iY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFEvOiEQvF2u M2Pve|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzO|Y4Lz5zOUC1N|c3PzxxYU6=
CCRF-SB NFXiUpJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NHiyXFI6PiCqcoO= M4\VSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ1PSSk1USiClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4yKM7:TT6= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2O{c,OTlyNUO3Olc9N2F-
HeLa NHjaUIlCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MkLhPVYhcHK| MnXQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB|IN88UU4> MoH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyNUO3OlcoRjF7MEWzO|Y4RC:jPh?=
HT-29 MWTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NGTQOHI6PiCqcoO= MnrLRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVD2yPUBk\WyuczDh[pRmeiB7NjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj61JO69VS5? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2O{c,OTlyNUO3Olc9N2F-
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MESSA MoLpRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MmDaOFghcHK| MmPKRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRWPTRUBk\WyuczDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhUUN3MDC9JFAvOyEQvF2u M2XBVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOUO5OVE3Lz5{MEmzPVUyPjxxYU6=
HL60 M1P6[WN6fG:2b4jpZ4l1gSCjc4PhfS=> NHXE[Gc4OiCqcoO= MmfzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVAhRSByLkS4OkDPxE1w NHLVXWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEm2NVc3Pyd-MkC5OlE4Pjd:L3G+
HL60/MX2 M2T1SWN6fG:2b4jpZ4l1gSCjc4PhfS=> MofwO|IhcHK| NWftd3JzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MD;NXFIh[2WubIOgZYZ1\XJiN{KgbJJ{NCCLQ{WwJF0hPDhwNTFOwG0v MnfSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7NkG3OlcoRjJyOU[xO|Y4RC:jPh?=
KB MYPDfZRwfG:6aXPpeJkh[XO|YYm= NHHFXZE{KGSjeYO= NYjzZoltS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFXDOVAhRSB2LkWg{txONg>? MnnxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyMki4PVgoRjJzMEK4PFk5RC:jPh?=
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502713 MUHBcpRq[2GwY3XyJIF{e2G7 MkHZOFghcHK| M2L6ZmFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjC1NFI4OTNiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvOSEQvF2u NGnNe4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC3OFQ1PCd-MkGwO|Q1PDR:L3G+
A549 NVrmRoY4SW62aXPhcoNmeiCjc4PhfS=> NF3XOVU1QCCqcoO= NIXJ[ZVCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gN{4xQCEQvF2u MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB5NES0OEc,OjFyN{S0OFQ9N2F-
DU145 NGHYO|FCdnSrY3HuZ4VzKGG|c3H5 NVLwOXM6PDhiaILz NVnuT4IySW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUA{NjdizszNMi=> NW\MeIZVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwO|Q1PDRpPkKxNFc1PDR2PD;hQi=>
HCT15 MV\BcpRq[2GwY3XyJIF{e2G7 MXu0PEBpenN? MUPBcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVUh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygTWM2OCB;IECuPUDPxE1w MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB5NES0OEc,OjFyN{S0OFQ9N2F-
Hep2 NIXRPG1CdnSrY3HuZ4VzKGG|c3H5 M2DYZVQ5KGi{cx?= M4HxdmFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZAzKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTCwMlkh|ryPLh?= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB5NES0OEc,OjFyN{S0OFQ9N2F-
IMR32 M4nuTGFvfGmlYX7j[ZIh[XO|YYm= NFPL[Xo1QCCqcoO= NIjafIhCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTW1TOzJiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFYvOyEQvF2u M3\XNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEe0OFQ1Lz5{MUC3OFQ1PDxxYU6=
PC3 MYnBcpRq[2GwY3XyJIF{e2G7 NVfiS|hvPDhiaILz MXnBcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFOzJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSB{Lk[g{txONg>? M2fSXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEe0OFQ1Lz5{MUC3OFQ1PDxxYU6=
HT-29 NHXE[4VEgXSxdH;4bYNqfHliYYPzZZk> NGGyPXA4OiCqcoO= MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBIUTVyIE2gN|EvPjJizszNMi=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTFzNUKxNEc,OjFzMUWyNVA9N2F-
K562 MYfDfZRwfG:6aXPpeJkh[XO|YYm= NHftTpE4OiCqcoO= MnLXR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBIUTVyIE2gNVIvPTlizszNMi=> NGfqb4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGxOVIyOCd-MkGxNVUzOTB:L3G+
MCF7 NEDT[pREgXSxdH;4bYNqfHliYYPzZZk> M{PUblczKGi{cx?= NFTHdGJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEeLNUCgQUAyQS57NTFOwG0v M3\OTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUG1NlExLz5{MUGxOVIyODxxYU6=
PC3 NVzUXHFnS3m2b4TvfIlkcXS7IHHzd4F6 MUe3NkBpenN? M3X0cWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBEOyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBIUTVyIE2gNE43OyEQvF2u NXPJZos1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNVUzOTBpPkKxNVE2OjFyPD;hQi=>
PC3 NHn6T3BEgXSxdH;4bYNqfHliYYPzZZk> M3K1cVczKGi{cx?= MnvNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGM{KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJHRIUSB;IEOuPVgh|ryPLh?= M1P5flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUG1NlExLz5{MUGxOVIyODxxYU6=
DU145 MmD6R5l1d3SxeHnjbZR6KGG|c3H5 NHvBOpJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCxMlQ1KM7:TT6= NFTQSXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGxOVI1Pid-MkGxNVUzPDZ:L3G+
HCT15 MmnKR5l1d3SxeHnjbZR6KGG|c3H5 NVvwdXpDS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUWgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNk4xOyEQvF2u NWrBfWF4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNVUzPDZpPkKxNVE2OjR4PD;hQi=>
K562 NWfBdJF4S3m2b4TvfIlkcXS7IHHzd4F6 MlLuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkKxJO69VS5? MlS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzMUWyOFYoRjJzMUG1NlQ3RC:jPh?=
MCF7 NGXJPGZEgXSxdH;4bYNqfHliYYPzZZk> MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDFwOUig{txONg>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTFzNUK0Okc,OjFzMUWyOFY9N2F-
95-D MnzvR5l1d3SxeHnjbZR6KGG|c3H5 NU\SSGhxPzJiaILz NXLWWXVUS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hQTVvRDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMkCyJO69VS5? M1ToRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUS1NVM6Lz5{MUG0OVE{QTxxYU6=
A549 Mo[4R5l1d3SxeHnjbZR6KGG|c3H5 MVS3NkBpenN? M1ftZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODB{NU[g{txONg>? MmLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzNEWxN|koRjJzMUS1NVM6RC:jPh?=
KB Ml\SR5l1d3SxeHnjbZR6KGG|c3H5 MWq3NkBpenN? NH:0WVlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4xODR4MTFOwG0v NVzwfG52RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxOFUyOzlpPkKxNVQ2OTN7PD;hQi=>
KB/VCR M{e4WGN6fG:2b4jpZ4l1gSCjc4PhfS=> MX[3NkBpenN? M1nwbGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDN1[FUjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yOEO0JO69VS5? NFT2NXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUG0OVE{QSd-MkGxOFUyOzl:L3G+
HepG2 M2LIXmN6fG:2b4jpZ4l1gSCjc4PhfS=> MVrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzNT64JO69VS5? NX;EcWNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxO|Q1ODhpPkKxNVc1PDB6PD;hQi=>
MOLT3 M3nvXmN6fG:2b4jpZ4l1gSCjc4PhfS=> NYjQeHB7S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVU:OVEOgZ4VtdHNiYomgXHRVKGG|c3H5MEBKSzVyIE2gNE4xOyEQvF2u MkW1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzN{S0NFgoRjJzMUe0OFA5RC:jPh?=
A549 MVfDfZRwfG:6aXPpeJkh[XO|YYm= NHHtdYZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGK7IGPSRkBie3OjeTygTWM2OCB;IEGuPFUh|ryPLh?= M1XqZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUiyNlU5Lz5{MUG4NlI2QDxxYU6=
SK-MEL-2 NXXtSlhrS3m2b4TvfIlkcXS7IHHzd4F6 M{K3XGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNU2HTD2yJINmdGy|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFEvOTdizszNMi=> MmfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzOEKyOVgoRjJzMUiyNlU5RC:jPh?=
SKOV3 NX3ZSGNYS3m2b4TvfIlkcXS7IHHzd4F6 MoDuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tQXjNiY3XscJMh[nliU2LCJIF{e2G7LDDJR|UxKD1iMT64NUDPxE1w NEfKNW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUG4NlI2QCd-MkGxPFIzPTh:L3G+
XF498 MnGyR5l1d3SxeHnjbZR6KGG|c3H5 MnvCR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gXGY1QThiY3XscJMh[nliU2LCJIF{e2G7LDDJR|UxKD1iMT63NkDPxE1w M2ToV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUiyNlU5Lz5{MUG4NlI2QDxxYU6=
A549 MXLDfZRwfG:6aXPpeJkh[XO|YYm= M{jEWVMh\GG7cx?= MnnuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyClZXzsJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCyNU41KM7:TT6= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ5N{[1OUc,OjF{N{e2OVU9N2F-
HCT116 MkfKR5l1d3SxeHnjbZR6KGG|c3H5 MVOzJIRigXN? NGPhVXlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOTNwNTFOwG0v M1P2dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMke3OlU2Lz5{MUK3O|Y2PTxxYU6=
HepG2 MorCR5l1d3SxeHnjbZR6KGG|c3H5 NV;ZOYlDOyCmYYnz MorjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEBoem:5dHigbY5pcWKrdHnvckBi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOE46KM7:TT6= NFrYSmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK3O|Y2PSd-MkGyO|c3PTV:L3G+
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DU145 MVTDfZRwfG:6aXPpeJkh[XO|YYm= MmXjNkBl[Xm| NYfFNopJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTFVzNEWgZ4VtdHNiYX\0[ZIhOiCmYYnzMEBKSzVyIE2gNk46PCEQvF2u NEXEVHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOxPFE3PCd-MkKzNVgyPjR:L3G+
HCT15 M4DOWGN6fG:2b4jpZ4l1gSCjc4PhfS=> MlrRNkBl[Xm| NWP1W5RkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUWgZ4VtdHNiYX\0[ZIhOiCmYYnzMEBKSzVyIE2gNU4{OyEQvF2u NILhVlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOxPFE3PCd-MkKzNVgyPjR:L3G+
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A2780 MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M33xT|Q5KGi{cx?= NIXHd2RIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygS2k2OCB;IEGuN|Eh|ryPLh?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN4NEe0Okc,OjJ|NkS3OFY9N2F-
A549 Mlf6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmDwOFghcHK| NHW1PWhIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFQ5KGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDHTVUxKD1iMz6wPEDPxE1w NV6wUZNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzOlQ4PDZpPkKyN|Y1PzR4PD;hQi=>
COLO205 NULycJRvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWO0PEBpenN? NV:4Unp6T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OT{KwOUBk\WyuczDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhT0l3MDC9JFAvOTNizszNMi=> M{PkNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{[0O|Q3Lz5{MkO2OFc1PjxxYU6=
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HEK293 M2jiUGN6fG:2b4jpZ4l1gSCjc4PhfS=> M3fuS|Q5KGi{cx?= MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDISWszQTNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhVEN3MDC9JFI5KM7:TT6= NH[yUFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO4NVA2OCd-MkKzPFExPTB:L3G+
DU145 MoXZR5l1d3SxeHnjbZR6KGG|c3H5 Mn;UNkBl[Xm| NHzCNpVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDj[YxteyCjZoTldkAzKGSjeYOgZpkh[2WubDDjc5VvfGmwZzDrbZQuQCCjbnHsfZNqeyxiSVO1NEA:KDFwMkGg{txONg>? NW\ScYJuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NFM3PTZpPkKyOVA{PjV4PD;hQi=>
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HEK293 Mn[yR5l1d3SxeHnjbZR6KGG|c3H5 MUiyJIRigXN? MlLWR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGVMOjl|IHPlcIx{KGGodHXyJFIh\GG7czDifUBk\WyuIHPveY51cW6pIHvpeE05KGGwYXz5d4l{NCCLQ{WwJF0hOS5{MTFOwG0v NVjZVIF6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NFM3PTZpPkKyOVA{PjV4PD;hQi=>
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PC3 NG\YUHdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MmDjO|IhcHK| Mn\yRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCSQ{OgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDFizszNMi=> M3vxb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkmxNFM4Lz5{NkK5NVA{PzxxYU6=
A549 MX\DfZRwfG:6aXPpeJkh[XO|YYm= MkXROFghcHK| M2HVNGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFM5NjVizszNMi=> M3P0flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkmyOlI5Lz5{NkK5NlYzQDxxYU6=
DU145 NXjE[HFGS3m2b4TvfIlkcXS7IHHzd4F6 MofMOFghcHK| MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOU4zOSEQvF2u MnO5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{OUK2NlgoRjJ4MkmyOlI5RC:jPh?=
HGC M{TiVGN6fG:2b4jpZ4l1gSCjc4PhfS=> Mke4OFghcHK| NXLWe|ZRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEeFIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAyOC57IN88UU4> NI\lfJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK5NlYzQCd-Mk[yPVI3Ojh:L3G+
MDA-MB-231 NYTlc4p7S3m2b4TvfIlkcXS7IHHzd4F6 NU\zbndzPDhiaILz MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB2OT6zOUDPxE1w NG\I[JE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK5NlYzQCd-Mk[yPVI3Ojh:L3G+
RWPE1 MoLTR5l1d3SxeHnjbZR6KGG|c3H5 NXzjb49XPDhiaILz MVPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSW3BGOSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNlcvPjRizszNMi=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ7Mk[yPEc,OjZ{OUK2Nlg9N2F-
HCT15 MV7DfZRwfG:6aXPpeJkh[XO|YYm= NUXIUoUzOiCmYYnz MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyPSClZXzsd{Bu\WG|dYLl[EBi\nSncjCyJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOU42PCEQvF2u NXXn[ZdHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zN|Q1QTlpPkK2N|M1PDl7PD;hQi=>
HeLa Mk\TR5l1d3SxeHnjbZR6KGG|c3H5 MlPpNkBl[Xm| NGHuOlNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{KG2nYYP1doVlKGGodHXyJFIh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUA4NjZizszNMi=> M1LiTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{O0OFk6Lz5{NkOzOFQ6QTxxYU6=
T47D MVnDfZRwfG:6aXPpeJkh[XO|YYm= MkXzNkBl[Xm| NF6wcVhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVPDeGIHPlcIx{KG2nYYP1doVlKGGodHXyJFIh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUA6NjJ6IN88UU4> NF\VcZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkOzOFQ6QSd-Mk[zN|Q1QTl:L3G+
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DU145 NVzsRlJyS3m2b4TvfIlkcXS7IHHzd4F6 Ml\GNkBl[Xm| M4XMRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IHPlcIx{KGGodHXyJFIh\GG7czDifUBES0t6IHHzd4F6NCCLQ{WwJF0hOTNwMTFOwG0v NWLES3Y2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zOlE4OzdpPkK2N|YyPzN5PD;hQi=>
HCT15 NE\lbWpEgXSxdH;4bYNqfHliYYPzZZk> M4jrW|Ih\GG7cx?= Mn;vR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTViY3XscJMh[W[2ZYKgNkBl[Xm|IHL5JGNEUzhiYYPzZZktKEmFNUCgQUAzNjZizszNMi=> MlHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|NkG3N|coRjJ4M{[xO|M4RC:jPh?=
MCF10A NWe5cllIS3m2b4TvfIlkcXS7IHHzd4F6 M3P6SlIh\GG7cx?= NGfyNFJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0ZzMFGgZ4VtdHNiYX\0[ZIhOiCmYYnzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSBzMD6xOEDPxE1w NW\YeYpHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zOlE4OzdpPkK2N|YyPzN5PD;hQi=>
T47D Mn7hR5l1d3SxeHnjbZR6KGG|c3H5 M{T0PVIh\GG7cx?= NGnsbY9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVPDeGIHPlcIx{KGGodHXyJFIh\GG7czDifUBES0t6IHHzd4F6NCCLQ{WwJF0hOC57NDFOwG0v Ml7tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|NkG3N|coRjJ4M{[xO|M4RC:jPh?=
HepG2 NYPlRVFUS3m2b4TvfIlkcXS7IHHzd4F6 MonkOFghcHK| M3THd2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUA{Oy57ODFOwG0v MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{O5N{c,OjZ|OUezPVM9N2F-
MOLT3 NYCyNZJyS3m2b4TvfIlkcXS7IHHzd4F6 MUW0PEBpenN? NWPZZlhxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVU:OVEOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFiWVDDhd5NigSxiSVO1NEA:KDBwMESxJO69VS5? NXy4NpdERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVc{QTNpPkK2N|k4Ozl|PD;hQi=>
A549 NHPP[m5EgXSxdH;4bYNqfHliYYPzZZk> NYLOS3p[PDhiaILz NWTrOoVxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nlicnXzZZp2emmwIHHzd4F6NCCLQ{WwJF0hOS55IN88UU4> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7Mki5O{c,OjZ3OUK4PVc9N2F-
DLD1 MUDDfZRwfG:6aXPpeJkh[XO|YYm= MnjKOFghcHK| M3\scmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRNTDFiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KHKnc3H6eZJqdiCjc4PhfUwhUUN3MDC9JFIh|ryPLh?= NG\XUHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5Nlg6Pyd-Mk[1PVI5QTd:L3G+
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CAKI-1 Mm\wRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NYDMRW96OjRidH:gO|IhcHK| NHfBdHZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFPBT2kuOSClZXzsd{Bi\nSncjCyOEB1dyB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iMT6xJO69VS5? MnHYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUW4O|UoRjJ4NUm1PFc2RC:jPh?=
HCT116 M1XOXWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NXryPGxsOjRidH:gO|IhcHK| NX3mXowxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgNlQhfG9iN{KgbJJ{KGK7IGPSRkBie3OjeTygTWM2OCB;IEOuN{DPxE1w NGLXZnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5OVg4PSd-Mk[1PVU5PzV:L3G+
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K562 M3;ufGN6fG:2b4jpZ4l1gSCjc4PhfS=> MW[3NkBpenN? Ml3zR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiSVO1NEA:KDFwMzFOwG0v Mnu1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6OUO1OVgoRjJ7OEmzOVU5RC:jPh?=
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SNU638 M2rZPGN6fG:2b4jpZ4l1gSCjc4PhfS=> NEPwRWk4OiCqcoO= NHeyNpNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUVlV4M{igZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO|YYmsJGlEPTBiPTCwMlQ{KM7:TT6= NHX2U4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUi5N|U2QCd-Mkm4PVM2PTh:L3G+
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LB2241-RCC MnznS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mlu0TWM2OCB;IEOxMlExOzJizszN NHzPdI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDZ3Nz:nQnNCVkeHUkyvZV4>
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MHH-CALL-2 MmLHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoPXTWM2OCB;IEO3MlM5ODhizszN MmP1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
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TE-441-T NEfob4ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2rBUGlEPTBiPTCzPU4yPDR5IN88US=> NFXQR|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDZ3Nz:nQnNCVkeHUkyvZV4>
KINGS-1 M2jkPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHvGS4VKSzVyIE2gN|kvOjd|MzFOwG0> NX;3dmtTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR4NUevK|5USU6JRWK8M4E,
NCI-H28 M2rjOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlLRTWM2OCB;IEO5MlQxODJizszN MoDtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
NCI-H1618 MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mm\ITWM2OCB;IESwMlExPDFizszN M2q2UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1PjV5Lze+V2FPT0WUPD;hQi=>
MFE-280 MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWL1TINtUUN3MDC9JFQxNjVyMEWg{txO MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFY2Py9pPmPBUmdGWjxxYU6=
EB-3 NXPtepdUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV;JR|UxKD1iNECuOlI1OyEQvF2= NFz6dHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDZ3Nz:nQnNCVkeHUkyvZV4>
IM-9 M3zJUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEHPTlZKSzVyIE2gOFEvPjN2MTFOwG0> NHTLN|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDZ3Nz:nQnNCVkeHUkyvZV4>
DV-90 NWTGPGJXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4XibGlEPTBiPTC0NU46PzRzIN88US=> NWnESFlwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR4NUevK|5USU6JRWK8M4E,
NCI-H1792 NILzc41Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXfiV2dzUUN3MDC9JFQzNjZ6NUig{txO MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFY2Py9pPmPBUmdGWjxxYU6=
SNU-387 NUPmZolxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1PUbmlEPTBiPTC0N{4zOThzIN88US=> MnrIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
HCC1395 NUfxd2FJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVfJR|UxKD1iNESuO|k2OyEQvF2= M3v1VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1PjV5Lze+V2FPT0WUPD;hQi=>
TGW NVu4Z2dNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGHiUIZKSzVyIE2gOFYvOzJ6OTFOwG0> NYOyZlJWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR4NUevK|5USU6JRWK8M4E,
KY821 NEG2[W9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4Hl[mlEPTBiPTC0O{4xQTN{IN88US=> MljiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
ECC4 NGPqVolIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVK0S3pXUUN3MDC9JFQ5NjBzOEKg{txO NILsOmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDZ3Nz:nQnNCVkeHUkyvZV4>
KARPAS-45 MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1\oWWlEPTBiPTC0PE4zOzV7IN88US=> NH;HdZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDZ3Nz:nQnNCVkeHUkyvZV4>
LB2518-MEL NUL0Wmp[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWHMTHpSUUN3MDC9JFQ5NjVyNU[g{txO MmnyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
COR-L88 NIr0[5hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1vTfGlEPTBiPTC0PE44OzVizszN MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFY2Py9pPmPBUmdGWjxxYU6=
COLO-741 NX;yUYV1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmiwTWM2OCB;IES4Mlg6PjZizszN MknoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+
HCE-T MnXhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWrkeWxrUUN3MDC9JFQ6NjNyMTFOwG0> M4TzeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1PjV5Lze+V2FPT0WUPD;hQi=>
NCI-H1755 NFjyVJpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NV;kRYRZUUN3MDC9JFQ6NjN2Nkig{txO MknxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ3PTdxJ{7TRW5ITVJ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p53 / p-p53 / Acetyl-p53 / TF-IIB; 

PubMed: 23800173     


Western blotting analysis of p53 levels and the indicated p53 post-translational modifications in the nucleus and cytoplasm of U-2 OS cells treated with no drug, 1 μmol/l etoposide (treated for 24 hours) and 100 μmol/l etoposide (treated for 12 hours), re䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ뙠ෆ䐺痖暼瘿뙠ෆᾰƌ 뙠ෆÐ㺣

p53 / p21 / c-Myc / PARP ; 

PubMed: 20212154     


Myc is repressed at the posttranscriptional level following etoposide treatment. HEK293 cells were treated with etoposide at the indicated concentrations for 24 h. Parallel samples were taken for western and northern analysis as indicated. 

23800173 20212154
Immunofluorescence
COX IV; 

PubMed: 29221178     


Panel 1 (untreated) depicts the mitochondrial distribution by visualizing the expression of Cox IV in U87(WT) cells. Panel 2 (etoposide treated) represents the distribution of mitochondria in U87(RETO) cells after 10 days of drug exposure as visualized by䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ

γH2AX / pATM ; 

PubMed: 19858003     


HeLa cells were treated with vehicle (Ctl), 6 Gy IR (IR) or 25 μM etoposide (ETO). Co-localization (yellow signal in the overlay (OL)) of gH2AX (red) and pATM (green). Scale bar = 20 μm.

MDC1; 

PubMed: 19858003     


HeLa cells were treated with vehicle (Ctl), 6 Gy IR (IR) or 25 μM etoposide (ETO). Co-localization of gH2AX with MDC1.

pDNA-PKcs; 

PubMed: 19858003     


HeLa cells were treated with vehicle (Ctl), 6 Gy IR (IR) or 25 μM etoposide (ETO). Co-localization of gH2AX with pDNA-PK.

p53; 

PubMed: 27515249     


p53 localization in untreated and 12-h etoposide treated CD41+ MK cells. Cells were stained with anti-p53 antibody. TOTO-3 was used to stain the nucleus. Scale bar=20 μm.

YAP; 

PubMed: 27515249     


(D) YAP localization in untreated CD41+MK cells and CD41+ MK cells treated by 10 μM etoposide for five hours on day 10 of culture. Cells were stained with anti-YAP antibody. DAPI was used to stain the nucleus. Cells (150) were counted and categorized acco䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ삨Ղ䐺痖暼瘿삨Ղᾰƌ 삨ՂÐ㺣痖삨Ղ€𢡄사Ղ€䀷痗사Ղ౴사Ղ㵶痗사Ղ뺖᎒泌Itemセ᎒Count﫨呂샜Ղ

29221178 19858003 27515249
Growth inhibition assay
Cell viability ; 

PubMed: 22615609     


Effect of etoposide on HEK293 cell viability. Cells were seeded at 104 in 96-well plates and treated with etoposide (50,100,150 and 200µM) for 24 hrs and compared to nontreated cells (control). At the end of treatment period, cell viability was measured u䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒

22615609
In vivo Etoposide administrated as a single agent is found to been ineffective in many xenografts growth, such as Heterotransplanted Hepatoblastoma NMHB1, and NMHB 2, [6] human neuroblastoma xenograft, [7] and human gastrointestinal cancer xenograft, [8] while the dose of 10 mg/kg i.p. Etoposide inhibits murine angiosarcoma cell ISOS-1 tumors in 36% of controls. [2] Etoposide induces tumor immunity in Lewis lung cancer. A single administration of 50 mg/kg Etoposide i.p., induces a 60% survival of C57B1/6 mice injected with Lewis lung cancer cell (3LL) over 60 days. About 40% of these surviving mice reject a subsequent challenge with 3LL, while none of control mice survive beyond 30 days. 3LL cells which have survived an 90% lethal concentration of Etoposide in vitro kill 75% of recipient mice, but 60% surviving mice reject challenge with 3LL. Splenocytes harvested from tumor rejecting mice protect naive mice injected with 3LL. [9]

Protocol

Kinase Assay:[5]
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Topoisomerase II activity assay:

Nuclear extracts are prepared, and nuclei are isolated. The activity of topoisomerase II is calculated from the percentage of decatenation obtained. Tritiated kinoplast DNA (KDNA 0.22 μg) is used as a substrate. Etoposide and topoisomerase II are incubated for 30 min at 37 ℃ and are stopped with 1% sodium dodecyl sulfate (SDS) and proteinase K (100 μg/mL). The percentages of decatenation and inhibition of topoisomerase II by Etoposide are obtained.
Cell Research:[5]
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  • Cell lines: Human glioma cell lines CL5
  • Concentrations: 80 μg/mL
  • Incubation Time: 1 hour
  • Method: After the Etoposide treatment, cells are removed from the dish with phosphate-buffered saline (PBS) containing 0.03% trypsin and 0.27 mM ethylenediaminetetraacetic acid (EDTA) and are diluted into culture dishes in appropriate numbers to yield between 20 and 200 colonies. After 12 days, cultures are fixed with methanol-acetic acid, stained with crystal violet, and scored for colonies containing more than 50 cells. The standard errors are typically less than 15% of the mean value unless otherwise stated.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Murine angiosarcoma xenografts ISOS-1
  • Dosages: 10 mg/kg
  • Administration: i.p. every day for 5 days from day 7
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (169.9 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
15mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 588.56
Formula

C29H32O13

CAS No. 33419-42-0
Storage powder
in solvent
Synonyms VP-16, VP-16213
Smiles CC1OCC2C(O1)C(C(C(O2)OC3C4COC(=O)C4C(C5=CC6=C(C=C35)OCO6)C7=CC(=C(C(=C7)OC)O)OC)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Regarding the Etoposide S1225, do you have any data of the inhibition sepcificity of this product? It will inhibit other enzymes other than TOP2A?

  • Answer:

    According to the available published data, the inhibition of Etoposide is specific to TOP2A. But there're also two papers showing that Etoposide could inhibit the p34cdc2 Kinase Activity: 1. http://cancerres.aacrjournals.org/content/52/7/1817.short ; 2. http://cancerres.aacrjournals.org/content/50/12/3761.short.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID