Chlorpromazine HCl

Catalog No.S2456 Synonyms: Sonazine

Chlorpromazine HCl Chemical Structure

Molecular Weight(MW): 355.33

Chlorpromazine HCl is a dopamine and potassium channel inhibitor with IC50 of 6.1 and 16 μM for inward-rectifying K+ currents and time-independent outward currents.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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Cited by 8 Publications

5 Customer Reviews

  • Low-micromolar amounts of chlorpromazine inhibit SARS-CoV-induced cytopathology. Vero E6 cells in 96-well plates were infected with SARS-CoV isolate Frankfurt-1 (MOI, 0.005) in the presence of 0 to 16  μM CPZ (B), given at t of +1 h p.i. Cells were incubated for 3 days, and viability was monitored using an MTS assay. In parallel, potential compound cytotoxicity was monitored in mock-infected Vero E6 cells. Graphs show the results (averages and SD) of a representative experiment that was performed in quadruplicate. All experiments were repeated at least twice. For each compound, the EC50, CC50, and SI values are given.

    Antimicrob Agents Chemother, 2014, 58:4875-4884.. Chlorpromazine HCl purchased from Selleck.

    Prussian blue staining for cells co-cultured with IOPs (25 mg/mL) and different inhibitors, (C1) control, (C2) LY294002 (100 μM, Selleck), (C3) genistein (10 μM, Selleck), (C4) chlorpromazine (10 μM, Selleck) for 24 h, arrows indicate the stained cells;

    Acta Biomater, 2018, 71:49-60. Chlorpromazine HCl purchased from Selleck.

  • Vero and H1299 cells were pretreated with increasing concentrations of CPZ (CME inhibitor), Amiloride (macropinocytosis inhibitor), or Nystatin (CavME inhibitor) for 30 min and infected with IBV at MOI= 1. H2O or DMSO pretreatment in parallel experiments was set as control. The incoming virus level was determined by quantification of viral gRNA at 2 h.p.i.; the expression level of IBV N protein was analyzed at 8 h.p.i.

    Virology, 2018, 528:118-136. Chlorpromazine HCl purchased from Selleck.

    RT-PCR assay of virus yield in the supernatants (infection group) and CIK cells (entry group). (unpaired t-test, * P < 0.05 and ** P < 0.01). Western blot analysis to monitor viral replication level in CIK cells.

    Virol J, 2018, 15(1):92. Chlorpromazine HCl purchased from Selleck.

  • Effects of inhibitors on GCRV infection. a Virus yield in CIK cells pretreated with vehicle, 10 mM NH4Cl, 20 μM CQ, 50 μM CPZ, or 100 μM of dynasore. b Western blotting analysis to monitor viral replication level. Cells were treated with various inhibitors as described above. After 12 h of incubation, cells were lysed and processed for Western blot analysis of GCRV-VP5 protein expression with polyclonal mouse serum. GAPDH was included as an internal loading control for the Western blots. NC denotes the negative control treated with vehicle. c Virus yield in CIK cells pretreated with vehicle, 5 μg/mL Filipin III, 50 μM LY294002, or 25 μg/mL nystatin. d Western blotting analysis to monitor the viral replication level. Cells were treated with inhibitors as described above. After 12 h of incubation, cells were lysed and processed for Western blot analysis of GCRV-VP5 protein expression with polyclonal mouse serum. GAPDH was included as an internal loading control for the Western blots. NC denotes the negative control treated with vehicle. Each experimental point is the sum of triplicate experiments with two titer assays. Error bars represent the standard errors of the mean. Asterisks represent a significant difference from the control (un-paired t-test)

    Virol J, 2016, 13:25.. Chlorpromazine HCl purchased from Selleck.

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Biological Activity

Description Chlorpromazine HCl is a dopamine and potassium channel inhibitor with IC50 of 6.1 and 16 μM for inward-rectifying K+ currents and time-independent outward currents.
Targets
Dopamine receptor [6] Potassium channel [6]
In vitro

Chlorpromazine affects miniature IPSCs (mIPSCs) by decreasing the binding (kon) and by increasing the unbinding (koff) rates of GABAARs. [1] Chlorpromazine modulates activated TRPA1 currents in a voltage-dependent way, leading to a block at positive potentials and an increased open probability at negative potentials. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CHO cells NYT2fXlETnWwY4Tpc44h[XO|YYm= M{\jSGNwdXCxdX7kJJdieyCndnHseYF1\WRiZn;yJJRp\SCkaX7kbY5oKGGoZnnubZR6KGGpYXnud5QhYzOKXUitU2guTFCDVD3sZYJmdGWmIEWtbJllem:6eYTyfZB1[W2rbnWgNWEhemWlZYD0c5Ihe2m2ZYOgbY4h[2yxbnXkJGNJVyClZXzsd{whU2l;MD62O|Mh|ryP NXrmbIdLQDB7N{WzPC=>
CHO cells MoDESpVv[3Srb36gZZN{[Xl? MULDc41xd3WwZDD3ZZMh\X[jbIXheIVlKG[xcjD0bIUh[mmwZHnu[{Bi\m[rbnn0fUBi\2GrboP0JHs{UF2XLUi2MFE4OC2uYXLlcIVlKER{IIPpeIV{KGmwIHPsc45m\CCFSF:gZ4VtdHNuIFvpQVAvODB|IN88US=> NGHIWpo5ODl5NUO4

... Click to View More Cell Line Experimental Data

In vivo Chlorpromazine independently down-regulates the production of various T cell-derived lymphokines (IL-2, IFN-gamma, IL-4, TNF, and GM-CSF) and up-regulates the secretion of IL-10 in an in vivo model of acute superantigen-driven immune activation. Chlorpromazine -mediated amplification of the SEB-driven Chlorpromazine secretion is accompanied by an enhanced IL-10 mRNA accumulation. [3] Chlorpromazine protects mice, normal or adrenalectomized, and guinea pigs against lethality of LPS, and inhibits TNF serum levels. Chlorpromazine protects against LPS lethality when administered 30 minutes (min) before, simultaneously, or up to 10 min after LPS and is ineffective when given 30 min after LPS, paralleling the inhibitory effect on TNF production. Chlorpromazine significantly inhibits LPS lethality and hepatotoxicity in mice sensitized to LPS toxicity by actinomycin D, whereas under these conditions DEX is inactive. [4] Chlorpromazine protects brain tissue from hypoxia-induced irreversible loss of synaptic transmission in rats. Chlorpromazine also significantly delays the occurrence of the hypoxia-induced spreading depression (SD) in rats. [5]

Protocol

Solubility (25°C)

In vitro DMSO 71 mg/mL (199.81 mM)
Water 71 mg/mL (199.81 mM)
Ethanol 71 mg/mL (199.81 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 355.33
Formula

C17H19ClN2S.HCl

CAS No. 69-09-0
Storage powder
in solvent
Synonyms Sonazine

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03548714 Completed Drug: PT150|Other: Beverage Alcohol Dependence|Alcohol Interaction Pop Test Oncology LLC|Pharmacotherapies for Alcohol and Substance Abuse Consortium|Congressionally Director Medical Research Programs|Michael Debakey Veterans Affairs Medical Center|Baylor College of Medicine|University of California San Diego September 1 2018 Phase 1
NCT02943213 Completed Drug: Chlorpromazine Hydrochloride Anti-Psychotic|Management of Manifestations of Psychotic Disorders|Treatment of Schizophrenia|Control Nausea and Vomiting|Relief of Restlessness and Apprehension Before Surgery|Acute Intermittent Porphyria|Adjunct in the Treatment of Tetanus|Control Manifestations of the Manic Type of Mani-depressive Illness|Relief of Intractable Hiccups Cycle Pharmaceuticals Ltd.|Parexel November 2016 Phase 1
NCT02295462 Completed Other: Person-centered Care|Other: Optimised Treatment Dementia Martin-Luther-Universität Halle-Wittenberg|University of Luebeck|University of Witten/Herdecke December 2014 Not Applicable

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Dopamine Receptor Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID