MPTP hydrochloride

Catalog No.S4732

For research use only.

MPTP hydrochloride is a dopaminergic neurotoxin and cause selective destruction of dopaminergic neurons in animal models of parkinsonism. MPTP hydrochloride induces apoptosis.

MPTP hydrochloride Chemical Structure

CAS No. 23007-85-4

Selleck's MPTP hydrochloride has been cited by 4 Publications

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Biological Activity

Description MPTP hydrochloride is a dopaminergic neurotoxin and cause selective destruction of dopaminergic neurons in animal models of parkinsonism. MPTP hydrochloride induces apoptosis.
In vitro

The morphology of N2AB-1 and glioma cells was unaltered when these cells were exposed to all doses of MPTP. And, C6 glioma cell proliferation was also unaffected by MPTP treatment[3]. MPTP Promotes Apoptosis and Tau Phosphorylation in Human Neuroblastoma M17 Cells. MPTP significantly promotes Tau phosphorylation at Ser262 in human neuroblastoma M17 cells. MPTP caused a dose-dependent increase in the intracellular α-synuclein level in our M17 human neuroblastoma cells. MPTP appears to promote Tau phosphorylation in the brain by activating both PKA and GSK3β[4].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
insect cells M4fOeGZ2dmO2aX;uJIF{e2G7 MXKxNlAhdWmwcx?= MXnJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KE2DT1Gg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczDhd5Nme3OnZDDhd{BwgGmmYYTpc44hd2Zia4nueZJidWmwZTDzeYJ{fHKjdHWgZZQhPTBidV2gcYVie3W{ZXSgZYZ1\XJiYXTkbZRqd26jbDDlcpp6dWViYXTk[YQh[W[2ZYKgNVIxKG2rboOgbY5kfWKjdHnvci=> MmHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{i0NVAoRjJ{MEe4OFExRC:jPh?=
insect cells MWTGeY5kfGmxbjDhd5NigQ>? MnrFPVAhdWmwcx?= MXjJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KE2DT1Gg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczDhd5Nme3OnZDDhd{BwgGmmYYTpc44hd2Zia4nueZJidWmwZTDzeYJ{fHKjdHWgZZQhPTBidV2gcYVie3W{ZXSgZYZ1\XJiYXTkbZRqd26jbDDzeYJ{fHKjdHWgZYRl\WRiYX\0[ZIhQTBibXnud{BqdmO3YnH0bY9v NGPUZoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC3PFQyOCd-MkKwO|g1OTB:L3G+
Assay
Methods Test Index PMID
Western blot TH / Actin ; pCaMKIIβ / pCaMKIIα / CaMKIIβ / CaMKIIα / β-Actin ; TH / p-α-Syn / α-Syn / β-actin / CDK5 / LC3-I / LC3-II / p62 / NLRP3 / ASC / Casp1 p20 / Procasp1 ; p-ERK1/2 / ERK1/2 / GAPDH ; GDH2 / GDH1 / GFAP / GAPDH 23391753 31427934 33717653 32927363 33093440
IHC tyrosine hydroxylase (TH) ; tyrosine hydroxylase (TH) ; SNpc ; tyrosine hydroxylase (TH) ; Substantia nigra 31427934 33717653 33093440 11724929 17336077
Immunofluorescence tyrosine hydroxylase (TH) / miR-188-3p ; α-Syn ; tyrosine hydroxylase (TH) / Tunel ; tyrosine hydroxylase (TH) / Tunel ; tyrosine hydroxylase (TH) ; GFAP 33717653 33093440
In vivo The number of tyrosine hydroxylase-positive neurons was decreased in the substantia nigra pars compacta of MPTP-treated mice. MPTP decreased thioredoxin reductase 1 expression and thioredoxin reductase activity in the mouse midbrain, reduced the number of thioredoxin reductase 1-positive cells in the substantia nigra pars compacta of mice. Administration of the toxin MPTP can cause neurochemical, behavioral and histopathological alterations in human and nonhuman primates that are similar to those observed in Parkinsonian patients. Compared with primates, rodents are insensitive to MPTP. MPTP can be administered by various routes, such as gavage and stereotactic injection, but the most common and reproducible route is systemic administration, including subcutaneous, intravenous, intraperitoneal and intramuscular injection. MPTP is a lipophilic protoxin that can rapidly cross the blood-brain barrier following systemic injection. Once it enters the brain, MPTP is converted to 1-methyl-4-phenylpyridine by monoamine oxidase B[1]. MPTP has been shown to be toxic to dopaminergic neurons of the nigrostriatal system in humans, monkeys, and mice and to produce long-lasting depletion of DA and its metabolites in the striatum[2].

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: N2AB-1 neural cell line and the C6 glioma cell line
  • Concentrations: 47.7, 4.77 and 0.477 μM
  • Incubation Time: 1, 2, 3 days
  • Method:

    N2AB-1 and C6 glioma cells were plated in 24-well costar dishes (16 mm diameter) at 50,000 cells per well with the culture medium described above. After 24 h medium was removed and medium with varying concentrations of MPTP or MPP+ was added in duplicate. Control and treated cells were then trypsinized and counted with a hemocytometer every day for 3 days following treatment.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: C57BL/6 mice
  • Dosages: 20 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

Water 41 mg/mL
(195.49 mM)
DMSO 20 mg/mL
(95.36 mM)
Ethanol '41 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 209.72
Formula

C12H15N·HCl

CAS No. 23007-85-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN1CCC(=CC1)C2=CC=CC=C2.Cl

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