MPTP hydrochloride

Catalog No.S4732

For research use only.

MPTP hydrochloride is a dopaminergic neurotoxin and cause selective destruction of dopaminergic neurons in animal models of parkinsonism. MPTP hydrochloride induces apoptosis.

MPTP hydrochloride Chemical Structure

CAS No. 23007-85-4

Selleck's MPTP hydrochloride has been cited by 4 Publications

Purity & Quality Control

Choose Selective Dopamine Receptor Inhibitors

Other Dopamine Receptor Products

Biological Activity

Description MPTP hydrochloride is a dopaminergic neurotoxin and cause selective destruction of dopaminergic neurons in animal models of parkinsonism. MPTP hydrochloride induces apoptosis.
In vitro

The morphology of N2AB-1 and glioma cells was unaltered when these cells were exposed to all doses of MPTP. And, C6 glioma cell proliferation was also unaffected by MPTP treatment[3]. MPTP Promotes Apoptosis and Tau Phosphorylation in Human Neuroblastoma M17 Cells. MPTP significantly promotes Tau phosphorylation at Ser262 in human neuroblastoma M17 cells. MPTP caused a dose-dependent increase in the intracellular α-synuclein level in our M17 human neuroblastoma cells. MPTP appears to promote Tau phosphorylation in the brain by activating both PKA and GSK3β[4].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
insect cells M4fOeGZ2dmO2aX;uJIF{e2G7 MXKxNlAhdWmwcx?= MXnJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KE2DT1Gg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczDhd5Nme3OnZDDhd{BwgGmmYYTpc44hd2Zia4nueZJidWmwZTDzeYJ{fHKjdHWgZZQhPTBidV2gcYVie3W{ZXSgZYZ1\XJiYXTkbZRqd26jbDDlcpp6dWViYXTk[YQh[W[2ZYKgNVIxKG2rboOgbY5kfWKjdHnvci=> MmHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{i0NVAoRjJ{MEe4OFExRC:jPh?=
insect cells MWTGeY5kfGmxbjDhd5NigQ>? MnrFPVAhdWmwcx?= MXjJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KE2DT1Gg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczDhd5Nme3OnZDDhd{BwgGmmYYTpc44hd2Zia4nueZJidWmwZTDzeYJ{fHKjdHWgZZQhPTBidV2gcYVie3W{ZXSgZYZ1\XJiYXTkbZRqd26jbDDzeYJ{fHKjdHWgZYRl\WRiYX\0[ZIhQTBibXnud{BqdmO3YnH0bY9v NGPUZoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC3PFQyOCd-MkKwO|g1OTB:L3G+
Methods Test Index PMID
Western blot TH / Actin ; pCaMKIIβ / pCaMKIIα / CaMKIIβ / CaMKIIα / β-Actin ; TH / p-α-Syn / α-Syn / β-actin / CDK5 / LC3-I / LC3-II / p62 / NLRP3 / ASC / Casp1 p20 / Procasp1 ; p-ERK1/2 / ERK1/2 / GAPDH ; GDH2 / GDH1 / GFAP / GAPDH 23391753 31427934 33717653 32927363 33093440
IHC tyrosine hydroxylase (TH) ; tyrosine hydroxylase (TH) ; SNpc ; tyrosine hydroxylase (TH) ; Substantia nigra 31427934 33717653 33093440 11724929 17336077
Immunofluorescence tyrosine hydroxylase (TH) / miR-188-3p ; α-Syn ; tyrosine hydroxylase (TH) / Tunel ; tyrosine hydroxylase (TH) / Tunel ; tyrosine hydroxylase (TH) ; GFAP 33717653 33093440
In vivo The number of tyrosine hydroxylase-positive neurons was decreased in the substantia nigra pars compacta of MPTP-treated mice. MPTP decreased thioredoxin reductase 1 expression and thioredoxin reductase activity in the mouse midbrain, reduced the number of thioredoxin reductase 1-positive cells in the substantia nigra pars compacta of mice. Administration of the toxin MPTP can cause neurochemical, behavioral and histopathological alterations in human and nonhuman primates that are similar to those observed in Parkinsonian patients. Compared with primates, rodents are insensitive to MPTP. MPTP can be administered by various routes, such as gavage and stereotactic injection, but the most common and reproducible route is systemic administration, including subcutaneous, intravenous, intraperitoneal and intramuscular injection. MPTP is a lipophilic protoxin that can rapidly cross the blood-brain barrier following systemic injection. Once it enters the brain, MPTP is converted to 1-methyl-4-phenylpyridine by monoamine oxidase B[1]. MPTP has been shown to be toxic to dopaminergic neurons of the nigrostriatal system in humans, monkeys, and mice and to produce long-lasting depletion of DA and its metabolites in the striatum[2].

Protocol (from reference)

Cell Research:


  • Cell lines: N2AB-1 neural cell line and the C6 glioma cell line
  • Concentrations: 47.7, 4.77 and 0.477 μM
  • Incubation Time: 1, 2, 3 days
  • Method:

    N2AB-1 and C6 glioma cells were plated in 24-well costar dishes (16 mm diameter) at 50,000 cells per well with the culture medium described above. After 24 h medium was removed and medium with varying concentrations of MPTP or MPP+ was added in duplicate. Control and treated cells were then trypsinized and counted with a hemocytometer every day for 3 days following treatment.

  • (Only for Reference)
Animal Research:


  • Animal Models: C57BL/6 mice
  • Dosages: 20 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

Water 41 mg/mL
(195.49 mM)
DMSO 20 mg/mL
(95.36 mM)
Ethanol '41 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 209.72


CAS No. 23007-85-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN1CCC(=CC1)C2=CC=CC=C2.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy MPTP hydrochloride | MPTP hydrochloride supplier | purchase MPTP hydrochloride | MPTP hydrochloride cost | MPTP hydrochloride manufacturer | order MPTP hydrochloride | MPTP hydrochloride distributor