Name: MPTP hydrochloride
Brand: Selleck Chemicals
SKU: S4732
Rating: 5 (17 reviews)

MPTP hydrochloride is a dopaminergic neurotoxin and cause selective destruction of dopaminergic neurons in animal models of parkinsonism. MPTP hydrochloride induces apoptosis.

MPTP hydrochloride Chemical Structure

CAS: 23007-85-4

Selleck's MPTP hydrochloride has been cited by 17 publications

Purity & Quality Control

Batch: Purity: 99.94%

99.94

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Signaling Pathway

Dopamine Receptor Signaling Pathway

Choose Selective Dopamine Receptor Inhibitors

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
insect cells	Function assay	120 mins	Inhibition of human recombinant MAOA expressed in insect cells assessed as oxidation of kynuramine substrate at 50 uM measured after additional enzyme added after 120 mins incubation	22078410
insect cells	Function assay	90 mins	Inhibition of human recombinant MAOA expressed in insect cells assessed as oxidation of kynuramine substrate at 50 uM measured after additional substrate added after 90 mins incubation	22078410
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Biological Activity

Description	MPTP hydrochloride is a dopaminergic neurotoxin and cause selective destruction of dopaminergic neurons in animal models of parkinsonism. MPTP hydrochloride induces apoptosis.

In vitro
In vitro	The morphology of N2AB-1 and glioma cells was unaltered when these cells were exposed to all doses of MPTP. And, C6 glioma cell proliferation was also unaffected by MPTP treatment^[3]. MPTP Promotes Apoptosis and Tau Phosphorylation in Human Neuroblastoma M17 Cells. MPTP significantly promotes Tau phosphorylation at Ser262 in human neuroblastoma M17 cells. MPTP caused a dose-dependent increase in the intracellular α-synuclein level in our M17 human neuroblastoma cells. MPTP appears to promote Tau phosphorylation in the brain by activating both PKA and GSK3β^[4].
Cell Research	Cell lines	N2AB-1 neural cell line and the C6 glioma cell line
	Concentrations	47.7, 4.77 and 0.477 μM
	Incubation Time	1, 2, 3 days
	Method	N2AB-1 and C6 glioma cells were plated in 24-well costar dishes (16 mm diameter) at 50,000 cells per well with the culture medium described above. After 24 h medium was removed and medium with varying concentrations of MPTP or MPP+ was added in duplicate. Control and treated cells were then trypsinized and counted with a hemocytometer every day for 3 days following treatment.
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	TH / Actin pCaMKIIβ / pCaMKIIα / CaMKIIβ / CaMKIIα / β-Actin TH / p-α-Syn / α-Syn / β-actin / CDK5 / LC3-I / LC3-II / p62 / NLRP3 / ASC / Casp1 p20 / Procasp1 p-ERK1/2 / ERK1/2 / GAPDH GDH2 / GDH1 / GFAP / GAPDH		23391753
	IHC	tyrosine hydroxylase (TH) tyrosine hydroxylase (TH) SNpc tyrosine hydroxylase (TH) Substantia nigra		31427934
	Immunofluorescence	tyrosine hydroxylase (TH) / miR-188-3p α-Syn tyrosine hydroxylase (TH) / Tunel tyrosine hydroxylase (TH) / Tunel tyrosine hydroxylase (TH) GFAP		33717653

In Vivo
In vivo	The number of tyrosine hydroxylase-positive neurons was decreased in the substantia nigra pars compacta of MPTP-treated mice. MPTP decreased thioredoxin reductase 1 expression and thioredoxin reductase activity in the mouse midbrain, reduced the number of thioredoxin reductase 1-positive cells in the substantia nigra pars compacta of mice. Administration of the toxin MPTP can cause neurochemical, behavioral and histopathological alterations in human and nonhuman primates that are similar to those observed in Parkinsonian patients. Compared with primates, rodents are insensitive to MPTP. MPTP can be administered by various routes, such as gavage and stereotactic injection, but the most common and reproducible route is systemic administration, including subcutaneous, intravenous, intraperitoneal and intramuscular injection. MPTP is a lipophilic protoxin that can rapidly cross the blood-brain barrier following systemic injection. Once it enters the brain, MPTP is converted to 1-methyl-4-phenylpyridine by monoamine oxidase B^[1]. MPTP has been shown to be toxic to dopaminergic neurons of the nigrostriatal system in humans, monkeys, and mice and to produce long-lasting depletion of DA and its metabolites in the striatum^[2].
Animal Research	Animal Models	C57BL/6 mice
	Dosages	20 mg/kg
	Administration	i.p.

References

[4] Qureshi HY, et al. J Biol Chem. 2011, 286(7):5055-68.

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Chemical Information & Solubility

Molecular Weight	209.72	Formula	C₁₂H₁₅N·HCl
CAS No.	23007-85-4	SDF	Download MPTP hydrochloride SDF
Smiles	CN1CCC(=CC1)C2=CC=CC=C2.Cl
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

Water : 41 mg/mL

Ethanol : 41 mg/mL

DMSO : 26 mg/mL ( (123.97 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

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In vivo
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