Catalog No.S7811

MHY1485 Chemical Structure

Molecular Weight(MW): 387.39

MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.

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Cited by 14 Publications

5 Customer Reviews

  • Exosomal miR-124-3p inhibited neuronal inflammation by suppressing the activity of mTOR signaling. A, B) Immunoblot (A) and quantitative (B) data of p-4E-BP1 and p-P70S6K in neurons after scratch injury and exosomal treatment. Their expression was increased after scratch injury and was suppressed by miR-124-3p–up-regulated exosomes, suggesting that miR-124-3p suppresses the activity of mTOR signaling (n = 6/group). ##P < 0.01 vs. control group; **P < 0.01 vs. injury group. C) Expression levels of inflammatory mediators in the culture medium were detected in the 3 groups of neurons: injured neurons (injury group), injured neurons treated with miR-124-3p–up-regulated exosomes (I+Exo-124), and injured neurons treated with miR-124-3p-up-regulated exosomes and the mTOR activator (MHY1485). Compared with the I+Exo-124 group, the MHY1485 group represented an increased expression on proinflammatory cytokines (TNF-α, IL-1β, and IL-6) and a decreased expression of anti-inflammatory cytokines (IL-10), suggesting that MHY1485 blocks the anti-inflammatory effect of miR-124-3p in injured neurons. Thus, the inhibitory effect of exosomal miR-124-3p on neuronal inflammation was exerted by suppressing the activity of mTOR signaling (n = 6/group). #P < 0.05 vs. injury group; *P < 0.05 vs. I+Exo-124 group.

    FASEB J, 2018, 32(1):512-528. MHY1485 purchased from Selleck.

  • Phosphorylation level of mTOR detected by western blot.

    Int J Biol Sci, 2017, 13(11):1398-1408. MHY1485 purchased from Selleck.

  • MYH1485, an activator of mTOR, reduced LC3II production and inhibited autophagosome formation in PAR2-knockdown cells.

    Biochem J, 2017, 474(16):2733-2747. MHY1485 purchased from Selleck.

  • The HO8910‑shLSD1 cells were treated with 10 μΜ mTOR specific activator MHY1485 for the indicated time‑points. The expression of p‑p70S6K and p70S6K was detected via western blot analysis.

    Oncol Rep, 2018, 40(1):425-433. MHY1485 purchased from Selleck.

  • Effects of liraglutide treatments and AMPK inhibitor/mTOR activator on matrix mineralization and relative protein levels of Alp, OC, p‑AMPK, p‑mTOR and TGF‑β. Following culture in commercial osteogenic differentiation medium for 14 days, MC3T3‑E1 cells were cultured for a further 14 days in basal culture medium with 4 nM liraglutide, and 4 nM liraglutide plus AMPK inhibitor, 10 μM compound C, or 1 μM mTOR activator, MHY1485. MC3T3‑E1 cells maintained in DMEM for 28 days with no treatment were used as the NC;cells cultured in the commercial osteogenic differentiation medium for 14 days and in DMEM without liraglutide for a further 14 days were used as the PC. (B) Relative protein expression was analyzed by western blot and quantifi ed relative to GAPDH (n=5). Data are presented as the mean ± standard error. Bars with letters means they signifi cantly differ with positive control (P<0.05). *P<0.05, **P<0.05 vs. NC; #P<0.05, ##P<0.01 vs. PC. NC, negative control; PC, positive control; Alp, alkaline phosphatase; OC, osteocalcin; p‑, phosphorylated; AMPK, adenosine monophosphate‑activated protein kinase; mTOR, mechanistic target of rapamycin; TGF‑β, transforming growth factor‑β; GAPDH, glyceraldehyde 3‑phosphate dehydrogenase.

    Mol Med Rep, 2016, 14(4):3662-8.. MHY1485 purchased from Selleck.

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Biological Activity

Description MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.
mTOR [1]
In vitro

MHY1485 suppresses the basal autophagic flux. MHY1485 causes the accumulation of LC3II and enlargement of the autophagosomes in a dose- and time- dependent manner.[1] MHY1485 increases phospho-mTOR levels and phosphorylation of downstream S6K1 and rpS6 proteins without affecting total mTOR content, total S6K1 and rpS6 levels. Short-term treatment of ovaries with MHY1485 followed by allo-transplantation promoted secondary follicle growth. Treatment with MHY1485 and subsequent grafting allowed the derivation of mature oocytes and healthy offspring.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9C2 NFTQTFNHfW6ldHnvckBie3OjeR?= Mnu5OUDPxE1? NV7ET|hYVUi\MUS4OUBi[nKxZ3H0[YQhfGinIHXm[oVkfHNib3[gWIFve2irbn;u[UBKUUFib36gUGM{ MU[zNFc6QDF|NB?=
RSC96 NFHnV3dHfW6ldHnvckBie3OjeR?= M3TuOlExKM7:TR?= NULX[2psOSCmYYmgc5IhOiCmYYnz NWPtc4dnVUi\MUS4OUBqdmO{ZXHz[YQheGixc4Doc{1uXE:UIDjT[ZIhOjR2ODmsJJBpd3OyaH;TOmsyKCiWaIKgN|g6MSCjbnSgUmdHKGW6cILld5Nqd25iaX6gVnNEQTZiY3XscJMv MlTzN|AzOjl|OUm=
HT-29  NI\LSJhHfW6ldHnvckBie3OjeR?= MXmxNEBvVSxiMUCwJI5ONCBzIN88UUwhOTBizszN NEi0fow1QCCq NWLqflRSe2mpbnnmbYNidnSueTDpcoNz\WG|ZXSgeIhmKGW6cILld5Nqd25ib3[gcYlTNTJzMjDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NV3BV3BsOzByMkGxNFA>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-mTOR / mTOR / p-AKT / AKT / p-S6K1 / S6K1 ; 

PubMed: 28061443     

Skin keratinocytes were treated with MHY1485 (10 μM) for 30 min, or plus mTOR kinase inhibitor (OSI-027, AZD2014 or AZD-8055, 200 nM each), expression of listed proteins was shown.

p-Nrf2 / Nrf2 ; 

PubMed: 28061443     

MHY1485 activates mTOR downstream Nrf2 signaling to protect skin keratinocytes from UV Skin keratinocytes were treated with MHY1485 (0.1-50 μM) for applied time, expression of listed proteins in cytosol and nuclei was shown.



Solubility (25°C)

In vitro DMSO 4 mg/mL (10.32 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 387.39


CAS No. 326914-06-1
Storage powder
in solvent
Synonyms N/A

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mTOR Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID