MHY1485

Catalog No.S7811

For research use only.

MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.

MHY1485 Chemical Structure

CAS No. 326914-06-1

Selleck's MHY1485 has been cited by 42 publications

Purity & Quality Control

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Biological Activity

Description MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.
Targets
mTOR [1]
In vitro

MHY1485 suppresses the basal autophagic flux. MHY1485 causes the accumulation of LC3II and enlargement of the autophagosomes in a dose- and time- dependent manner.[1] MHY1485 increases phospho-mTOR levels and phosphorylation of downstream S6K1 and rpS6 proteins without affecting total mTOR content, total S6K1 and rpS6 levels. Short-term treatment of ovaries with MHY1485 followed by allo-transplantation promoted secondary follicle growth. Treatment with MHY1485 and subsequent grafting allowed the derivation of mature oocytes and healthy offspring.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT-29  MoLJSpVv[3Srb36gZZN{[Xl? MkPWNVAhdk1uIEGwNEBvVSxiMTFOwG0tKDFyIN88US=> MomwOFghcA>? MVrzbYdvcW[rY3HueIx6KGmwY4LlZZNm\CC2aHWg[ZhxemW|c3nvckBw\iCvaWKtNlEzKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MoOzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByMkGxNFAoRjNyMEKxNVAxRC:jPh?=
RSC96 NVe4TlByTnWwY4Tpc44h[XO|YYm= NF7QU3YyOCEQvF2= NEO1OFEyKGSjeTDvdkAzKGSjeYO= M3LtUm1JYTF2OEWgbY5kemWjc3XkJJBpd3OyaH:tcXRQWiBqU3XyJFI1PDhrLDDwbI9{eGixU{\LNUApXGi{IEO4PUkh[W6mIF7HSkBmgHC{ZYPzbY9vKGmwIGLTR|k3KGOnbHzzMi=> NWHCO2pMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNlk{QTlpPkOwNlI6Ozl7PD;hQi=>
H9C2 MoC2SpVv[3Srb36gZZN{[Xl? M1jBe|Uh|ryP NX30U5hTVUi\MUS4OUBi[nKxZ3H0[YQhfGinIHXm[oVkfHNib3[gWIFve2irbn;u[UBKUUFib36gUGM{ MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODd7OEGzOEc,OzB5OUixN|Q9N2F-
Assay
Methods Test Index PMID
Western blot p-mTOR / mTOR / p-AKT / AKT / p-S6K1 / S6K1 ; p-Nrf2 / Nrf2 28061443

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 4 mg/mL
(10.32 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 387.39
Formula

C17H21N7O4

CAS No. 326914-06-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1COCCN1C2=NC(=NC(=N2)NC3=CC=C(C=C3)[N+](=O)[O-])N4CCOCC4

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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