Catalog No.S7811

For research use only.

MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.

MHY1485 Chemical Structure

CAS No. 326914-06-1

Selleck's MHY1485 has been cited by 50 publications

Purity & Quality Control

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Biological Activity

Description MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.
mTOR [1]
In vitro

MHY1485 suppresses the basal autophagic flux. MHY1485 causes the accumulation of LC3II and enlargement of the autophagosomes in a dose- and time- dependent manner.[1] MHY1485 increases phospho-mTOR levels and phosphorylation of downstream S6K1 and rpS6 proteins without affecting total mTOR content, total S6K1 and rpS6 levels. Short-term treatment of ovaries with MHY1485 followed by allo-transplantation promoted secondary follicle growth. Treatment with MHY1485 and subsequent grafting allowed the derivation of mature oocytes and healthy offspring.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT-29  M37HfmZ2dmO2aX;uJIF{e2G7 NYHnZmI3OTBibl2sJFExOCCwTTygNUDPxE1uIEGwJO69VQ>? NYrCT493PDhiaB?= M2DhWZNq\26rZnnjZY51dHliaX7jdoVie2WmIITo[UBmgHC{ZYPzbY9vKG:oIH3pVk0zOTJiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB{MUGwNEc,OzByMkGxNFA9N2F-
RSC96 MkHsSpVv[3Srb36gZZN{[Xl? NUjlS3FGOTBizszN M4G4b|Eh\GG7IH;yJFIh\GG7cx?= NWGxb|VtVUi\MUS4OUBqdmO{ZXHz[YQheGixc4Doc{1uXE:UIDjT[ZIhOjR2ODmsJJBpd3OyaH;TOmsyKCiWaIKgN|g6MSCjbnSgUmdHKGW6cILld5Nqd25iaX6gVnNEQTZiY3XscJMv NWjLVWx5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNlk{QTlpPkOwNlI6Ozl7PD;hQi=>
H9C2 NFnGb4RHfW6ldHnvckBie3OjeR?= NF\QOVI2KM7:TR?= NIPPN|lOUFlzNEi1JIFjem:pYYTl[EB1cGViZX\m[YN1eyCxZjDUZY5{cGmwb37lJGlKSSCxbjDMR|M> M{jJSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyN{m4NVM1Lz5|MEe5PFE{PDxxYU6=
Methods Test Index PMID
Western blot p-mTOR / mTOR / p-AKT / AKT / p-S6K1 / S6K1 ; p-Nrf2 / Nrf2 28061443

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 387.39


CAS No. 326914-06-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1COCCN1C2=NC(=NC(=N2)NC3=CC=C(C=C3)[N+](=O)[O-])N4CCOCC4

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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