Torin 1

Catalog No.S2827

Torin 1 Chemical Structure

Molecular Weight(MW): 607.62

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

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Cited by 98 Publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 NEL2Sm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHpTWM2OD1yLkCwNFMxPiEQvF2= MXLTRW5ITVJ?
OCUB-M NGKyO3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rPTGlEPTB;MD6wNFE6PCEQvF2= MV;TRW5ITVJ?
DOHH-2 NV7SPYppT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LiUmlEPTB;MD6wNFIzPSEQvF2= MkPLV2FPT0WU
NOS-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{[zZmlEPTB;MD6wNFI1OiEQvF2= M{e3N3NCVkeHUh?=
L-363 M{nnSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\zPGtKSzVyPUCuNFAzQDRizszN MnTtV2FPT0WU
RL95-2 M3fMdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\QcGlEPTB;MD6wNFMzKM7:TR?= MYDTRW5ITVJ?
BL-70 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTlTXhSUUN3ME2wMlAxOzN5IN88US=> Mm\2V2FPT0WU
TGBC1TKB NGPEUI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLufWZQUUN3ME2wMlAxOzd7IN88US=> Ml;mV2FPT0WU
697 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3u4PWlEPTB;MD6wNFY{PCEQvF2= NYLsfpFvW0GQR1XS
RPMI-8226 M2TWNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnq1TWM2OD1yLkCwOlUyKM7:TR?= NXLRdWdJW0GQR1XS
BL-41 NEfISFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDsfGJKSzVyPUCuNFA3QTZizszN NGi4PGdUSU6JRWK=
MRK-nu-1 NUPudJJnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfuTWM2OD1yLkCwO|A4KM7:TR?= M1LHSXNCVkeHUh?=
EW-1 NIK5TXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17MPGlEPTB;MD6wNFc1QSEQvF2= Ml3XV2FPT0WU
EVSA-T NFrGeo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jtSmlEPTB;MD6wNFg6OiEQvF2= M{jYXnNCVkeHUh?=
HUTU-80 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LsR2lEPTB;MD6wNFkzQSEQvF2= M4\zeXNCVkeHUh?=
VA-ES-BJ MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfOTWM2OD1yLkCwPVg1KM7:TR?= NVq2TJBzW0GQR1XS
GCIY NVnxNmFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTBwMEGwNFch|ryP M{PMU3NCVkeHUh?=
ES6 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7NTWM2OD1yLkCxNFg{KM7:TR?= NHezdmJUSU6JRWK=
NALM-6 M13lWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXnSohKSzVyPUCuNFEyODJizszN MVTTRW5ITVJ?
LB996-RCC MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7vNoszUUN3ME2wMlAyOTN5IN88US=> NGDWSFhUSU6JRWK=
EW-16 MnvyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jCZmlEPTB;MD6wNVE6OiEQvF2= MlLzV2FPT0WU
KALS-1 MlzqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTSd5RoUUN3ME2wMlAyOTl6IN88US=> NH;r[HlUSU6JRWK=
OPM-2 M3q0eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\LVVdKSzVyPUCuNFEzQSEQvF2= MV\TRW5ITVJ?
KU812 MlvqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\BbWlEPTB;MD6wNVI6PiEQvF2= MW\TRW5ITVJ?
A4-Fuk MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVz5bWN2UUN3ME2wMlAyOzF7IN88US=> NXfxT21QW0GQR1XS
KGN MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHtdppKSzVyPUCuNFE{PTlizszN NH;Wc5VUSU6JRWK=
KS-1 NUX4UWtNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnicINTUUN3ME2wMlAyOzl{IN88US=> NVG5c2h2W0GQR1XS
no-11 M3XtUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULEemlvUUN3ME2wMlAyOzl3IN88US=> Ml6zV2FPT0WU
PSN1 NUHQfJZWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTBwMEG0NFkh|ryP NEj3[pZUSU6JRWK=
SK-LMS-1 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnZd|R3UUN3ME2wMlAyPDF|IN88US=> NHviOWZUSU6JRWK=
LS-411N MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPRTWM2OD1yLkCxOFM6KM7:TR?= NGLNUHlUSU6JRWK=
HT-144 NGjBc5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTBwMEG0OVEh|ryP NGPIRVVUSU6JRWK=
ACN M1P4VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPqR2ZoUUN3ME2wMlAyPSEQvF2= MlvQV2FPT0WU
RKO NF;CRmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnziTWM2OD1yLkCxOVM{KM7:TR?= MkHRV2FPT0WU
CMK MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTBwMEG1OFMh|ryP NWfWTVdQW0GQR1XS
GB-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{HaU2lEPTB;MD6wNVU2OyEQvF2= NUntOHE3W0GQR1XS
KE-37 MnWwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3ENo9wUUN3ME2wMlAyPTd|IN88US=> MX3TRW5ITVJ?
no-10 MnLGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTBwMEG1PFQh|ryP NYe2N4lnW0GQR1XS
MOLT-16 MmDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwMEG2NlIh|ryP MkK1V2FPT0WU
HEL NVHQbWZKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTBwMEG2OEDPxE1? MXzTRW5ITVJ?
NCI-H1355 NEfMUoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLHVI1KSzVyPUCuNFE3PDZizszN M4T1UnNCVkeHUh?=
IST-MEL1 M3:wcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7pZWJKSzVyPUCuNFE4PzRizszN MXjTRW5ITVJ?
MHH-PREB-1 M{DDb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDyN5hKSzVyPUCuNFE5OThizszN M3PYN3NCVkeHUh?=
ES8 M3Plemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfSem5KSzVyPUCuNFE5PDRizszN NW\Se3J4W0GQR1XS
LC-2-ad NXTTOlJxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjsO25ZUUN3ME2wMlAzOTN5IN88US=> MW\TRW5ITVJ?
LAMA-84 NFziV2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LRN2lEPTB;MD6wNlE3PyEQvF2= M4Pne3NCVkeHUh?=
BV-173 MmrtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTBwMEKyNFMh|ryP MV;TRW5ITVJ?
SW872 M2K0TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIf0VlBKSzVyPUCuNFIzPDdizszN NUfFRmRmW0GQR1XS
SK-UT-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2f1SWlEPTB;MD6wNlI3OSEQvF2= NWrHfXRTW0GQR1XS
OS-RC-2 NHzUXHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYnMR5AxUUN3ME2wMlAzOzJ|IN88US=> NGDoXZBUSU6JRWK=
NCI-H747 NHG5UWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXiwZlR5UUN3ME2wMlAzOzNizszN NIW0RWxUSU6JRWK=
MZ2-MEL M{DzW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{PBUWlEPTB;MD6wNlM{PyEQvF2= NFvLcnBUSU6JRWK=
TE-11 NHGxVZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHSZVk3UUN3ME2wMlAzOzd{IN88US=> NF[4NmFUSU6JRWK=
KURAMOCHI NULaU4ptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\FTWM2OD1yLkCyOFE1KM7:TR?= NECwWpNUSU6JRWK=
HD-MY-Z NYnmU4VlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn25TWM2OD1yLkCyOFI3KM7:TR?= NGnndppUSU6JRWK=
GI-1 NIC3R|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7pS3N{UUN3ME2wMlAzPDZ7IN88US=> NX7FS2dnW0GQR1XS
LB1047-RCC NFPiN3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHZfmZNUUN3ME2wMlAzPDh6IN88US=> NUDOVXdbW0GQR1XS
TE-12 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUD3XnZQUUN3ME2wMlAzPTVizszN M4Pn[nNCVkeHUh?=
MZ7-mel NF\5SZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzUXYxKSzVyPUCuNFI3ODlizszN NXjwXpZMW0GQR1XS
MMAC-SF NV7XSI5MT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3n3OWlEPTB;MD6wNlY{OyEQvF2= NV6xS|BpW0GQR1XS
A101D MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoX1TWM2OD1yLkCyOlUh|ryP MkDhV2FPT0WU
GR-ST NGjUXWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTzTWM2OD1yLkCyOlU2KM7:TR?= NES1UGJUSU6JRWK=
MC116 MkTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTBwMEK2PEDPxE1? NFjpfY5USU6JRWK=
Ramos-2G6-4C10 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4X5dWlEPTB;MD6wNlc2KM7:TR?= NVXpWHB7W0GQR1XS
ATN-1 NGLSR4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvyTWM2OD1yLkCyO|g6KM7:TR?= MWPTRW5ITVJ?
MFH-ino NVP5S|F7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV64dVR5UUN3ME2wMlAzQDF{IN88US=> NF3LOm9USU6JRWK=
DEL NXLXeHNnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTBwMEK4NVUh|ryP NYPUPWtFW0GQR1XS
OCI-AML2 MmTqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37KemlEPTB;MD6wNlg4OSEQvF2= MVXTRW5ITVJ?
LXF-289 MoDES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrxTWM2OD1yLkCyPFczKM7:TR?= MWPTRW5ITVJ?
IA-LM NEXTbIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnnaTWM2OD1yLkCyPVE4KM7:TR?= NUHHTYJXW0GQR1XS
EoL-1-cell MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPpeGJqUUN3ME2wMlAzQTl4IN88US=> NVjMc2tWW0GQR1XS
OVCAR-4 M3f4cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TxUGlEPTB;MD6wN|A6PCEQvF2= NFHGTW9USU6JRWK=
ES1 MkTZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7t[VZKSzVyPUCuNFMxQTdizszN M{HWUXNCVkeHUh?=
CESS MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTBwMEOwPVkh|ryP MWHTRW5ITVJ?
GAK NUXPVJh5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn74TWM2OD1yLkCzNVg3KM7:TR?= NWTpRpg3W0GQR1XS
NTERA-S-cl-D1 NVz0TYhbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTBwMEOyNlYh|ryP Ml;KV2FPT0WU
TE-10 M{XyeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3myVWlEPTB;MD6wN|MyOSEQvF2= NV3kVWNvW0GQR1XS
CCRF-CEM M3\mS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHlTWM2OD1yLkCzN|cyKM7:TR?= MknjV2FPT0WU
NMC-G1 MkHDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknyTWM2OD1yLkCzOFQ5KM7:TR?= MVPTRW5ITVJ?
HOP-62 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULS[pRWUUN3ME2wMlA{PTV4IN88US=> NFHqToJUSU6JRWK=
SH-4 MkfOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvaOnFKSzVyPUCuNFM2QDJizszN NFvZfHBUSU6JRWK=
NCI-H1648 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzhWnVRUUN3ME2wMlA{PjJ{IN88US=> NIfQclBUSU6JRWK=
EB-3 MoraS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHT3SFhKSzVyPUCuNFM3PDNizszN NWPrU4NxW0GQR1XS
BB65-RCC NYfTPW9sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUG2XWpLUUN3ME2wMlA{PjlzIN88US=> MWDTRW5ITVJ?
BE-13 NIW2elhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XTUWlEPTB;MD6wN|c2QSEQvF2= M3vuVXNCVkeHUh?=
CP67-MEL NV3CZ5dlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nLeWlEPTB;MD6wN|c2QSEQvF2= Mn3yV2FPT0WU
SW954 NFm5d3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;6U4RrUUN3ME2wMlA{PzlizszN NHPWV3VUSU6JRWK=
TE-5 MnTCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\hWY9KSzVyPUCuNFM5PTRizszN MYjTRW5ITVJ?
HCE-4 NIDLTG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTBwMEO4OVUh|ryP NFPrPG5USU6JRWK=
LC-1F M3[wSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFXPO3NKSzVyPUCuNFM6QDlizszN MY\TRW5ITVJ?
TK10 NIftRYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrlZlNKUUN3ME2wMlA1ODF7IN88US=> NGDCVpRUSU6JRWK=
SJSA-1 NHixRlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3:xOWlEPTB;MD6wOFE5QCEQvF2= NFrSWIxUSU6JRWK=
Daudi M122d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrEPINKSzVyPUCuNFQzOjVizszN MXLTRW5ITVJ?
SW982 Mke3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\2cHh3UUN3ME2wMlA1OjR4IN88US=> Ml[yV2FPT0WU
SF126 MmTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\hTWM2OD1yLkC0N|YyKM7:TR?= MnTwV2FPT0WU
TE-15 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY[0b3prUUN3ME2wMlA1PDN3IN88US=> M{\ifHNCVkeHUh?=
COLO-684 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{HBT2lEPTB;MD6wOFU3OSEQvF2= M4HmV3NCVkeHUh?=
8-MG-BA MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTBwMES2Nlkh|ryP NET4VmdUSU6JRWK=
NB13 NWjMVogzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTBwMES3O|Yh|ryP MnzxV2FPT0WU
HCC1599 NWXiN4ZYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojSTWM2OD1yLkC0PFc6KM7:TR?= M2HiNnNCVkeHUh?=
SK-PN-DW NIL6[FVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvXTHBKSzVyPUCuNFQ6PDlizszN NFLqS5BUSU6JRWK=
ALL-PO MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LEfmlEPTB;MD6wOFk2PiEQvF2= NYjuRmRzW0GQR1XS
NB6 NIDMRWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTBwMEWyO|Eh|ryP MmjTV2FPT0WU
IST-SL2 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3wTWM2OD1yLkC1N|YzKM7:TR?= M3HYNnNCVkeHUh?=
BB30-HNC Mn;oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTBwMEW3N{DPxE1? NFK2c2ZUSU6JRWK=
SW962 M2riTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;rN4VOUUN3ME2wMlA2PzZ|IN88US=> MUTTRW5ITVJ?
TGBC24TKB MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{W0XmlEPTB;MD6wOVc6QSEQvF2= NHjGW5dUSU6JRWK=
KINGS-1 Mn3vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\zUWlEPTB;MD6wOVgxOyEQvF2= NFv3fFBUSU6JRWK=
HC-1 NWC0SpBYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\GSWFKSzVyPUCuNFU5OjdizszN NUjtTmtGW0GQR1XS
RS4-11 MmnFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTBwMEW5N|gh|ryP MoLFV2FPT0WU
LS-123 MkfMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4W2ZmlEPTB;MD6wOVk1OiEQvF2= NFLPRZBUSU6JRWK=
A3-KAW Mnm2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTBwME[yNlUh|ryP NGHUTppUSU6JRWK=
SNB75 MknYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LHemlEPTB;MD6wOlM1OSEQvF2= MW\TRW5ITVJ?
SR M{XCfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;zbGlEPTB;MD6wOlM4PCEQvF2= M33aenNCVkeHUh?=
LOUCY M1fVdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXuyRXRYUUN3ME2wMlA3Ozl2IN88US=> Mon6V2FPT0WU
K5 M2jWWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTBwME[0NFMh|ryP Moq1V2FPT0WU
Becker MlPlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HlfWlEPTB;MD6wOlQzKM7:TR?= MmixV2FPT0WU
CTV-1 M1\idGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjPR5BlUUN3ME2wMlA3PTR6IN88US=> NGH5V2RUSU6JRWK=
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NCI-H187 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljTTWM2OD1yLkS3PFQh|ryP MWrTRW5ITVJ?
DB NYDhVIxWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnJUplKSzVyPUCuOVAxPTVizszN NUHqcHZYW0GQR1XS
NCI-H69 NGfLfnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV7JR|UxRTBwNUCyOFch|ryP NUfmNWZNW0GQR1XS
NCI-H1694 NID2TWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTBwNUC2PVch|ryP MWjTRW5ITVJ?
CA46 MmjZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTBwNUC5NVch|ryP NH\hO4hUSU6JRWK=
EHEB MkfhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTBwNUGxPVMh|ryP Ml7uV2FPT0WU
NCCIT MkSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrMdo4yUUN3ME2wMlU1OzJzIN88US=> M{jLcHNCVkeHUh?=
NCI-H720 NUS2TXZmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXuSIhKSzVyPUCuOVQ6PjJizszN NU\Ed3VmW0GQR1XS
LU-134-A NFXmTY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmC5TWM2OD1yLkW1Nlk3KM7:TR?= MmD3V2FPT0WU
MC-CAR MlPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXzmN3lSUUN3ME2wMlU2PDNzIN88US=> M3;ibHNCVkeHUh?=
NEC8 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fzTmlEPTB;MD61OlE4OiEQvF2= MorPV2FPT0WU
NCI-H524 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnWOlBKSzVyPUCuOVg4PDhizszN NUfEN5JqW0GQR1XS
NCI-H1963 NHW0V2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVX2UnlmUUN3ME2wMlYzPThizszN NYj0TII3W0GQR1XS
SU-DHL-1 NH;nVW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXoTWM2OD1yLk[0OVI3KM7:TR?= NWPTNY5qW0GQR1XS
NCI-H1522 NXe2VmJOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3lTWM2OD1yLk[0PFg{KM7:TR?= M1vkbnNCVkeHUh?=
NCI-H1155 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHzXm9KSzVyPUCuOlU{PSEQvF2= MV;TRW5ITVJ?
D-263MG NHjnNmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoD0TWM2OD1yLk[2NVAyKM7:TR?= M4TOZXNCVkeHUh?=
TE-6 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHzbHJlUUN3ME2wMlY5OTB2IN88US=> MXzTRW5ITVJ?
NCI-H1417 NUe2XG5sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLqPFdzUUN3ME2wMlY6PzFzIN88US=> M4TqOnNCVkeHUh?=
NCI-H510A MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTBwN{eyN|Yh|ryP MmDnV2FPT0WU
KARPAS-45 NXfLWXlET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrvc2ZtUUN3ME2wMlc5OjN3IN88US=> M3;jZXNCVkeHUh?=
IST-SL1 Mk[2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFroZppKSzVyPUCuPFU5OjVizszN MmDGV2FPT0WU
COLO-824 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1K1VGlEPTB;MD64O|g{PSEQvF2= MlPLV2FPT0WU
NCI-H526 MmPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTBwOUC5OVgh|ryP M1vhcHNCVkeHUh?=
NCI-H719 NWLXXokyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mne5TWM2OD1yLkm2OlU4KM7:TR?= MXvTRW5ITVJ?
SF539 MnnZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnnXGRKSzVyPUCuPVg{QTZizszN NFXTeplUSU6JRWK=
NKM-1 M2HCbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjRTWM2OD1yLkm4OlY{KM7:TR?= M2\DOHNCVkeHUh?=
A253 MlXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjpV3RDUUN3ME2wMlk5QDh3IN88US=> MXHTRW5ITVJ?
TE-441-T NV7YNWd1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDTTWM2OD1zLkC0N|Uh|ryP NWPnWVFJW0GQR1XS
COR-L88 MnHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXq2OHpmUUN3ME2xMlEyOzV2IN88US=> NHnZVIRUSU6JRWK=
CPC-N NYDONo5bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXtR2NpUUN3ME2xMlE2QDh6IN88US=> M3OwNHNCVkeHUh?=
NCI-H2107 NFW1SXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{Lh[WlEPTB;MT6xO|I3OSEQvF2= M4TXSXNCVkeHUh?=
ECC4 M2T4Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn[5TWM2OD1zLkG4Olk{KM7:TR?= MV;TRW5ITVJ?
NCI-H2141 M{Ltc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTFwMUmyPVkh|ryP NF61TmVUSU6JRWK=
NCI-H1770 M3H2Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLXTWM2OD1zLkOyPVI5KM7:TR?= NGC5bGtUSU6JRWK=
NCI-SNU-1 NVXsT2VHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInEdXFKSzVyPUGuN|MxPCEQvF2= MlflV2FPT0WU
LU-165 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTFwNEW0Olch|ryP NVm3TG05W0GQR1XS
Raji M1\JVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoO0TWM2OD1zLkS3NlU6KM7:TR?= MWnTRW5ITVJ?
EW-24 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnOOpRWUUN3ME2xMlU1PjF7IN88US=> MX7TRW5ITVJ?
KP-N-RT-BM-1 MmrVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjLfGdDUUN3ME2xMlU3PTVzIN88US=> MVfTRW5ITVJ?
SK-MEL-1 NHjLSVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1vkNGlEPTB;MT62OFg4PyEQvF2= M2jtXXNCVkeHUh?=
SCLC-21H NF62[VVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvNTWM2OD1zLk[2OlUyKM7:TR?= Ml;tV2FPT0WU
SIMA MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHMfIhKSzVyPUGuOlgzOzNizszN M4f3fnNCVkeHUh?=
NCI-H1304 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NInIUIpKSzVyPUGuO|A4PjVizszN MYDTRW5ITVJ?
REH MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUHmVGRDUUN3ME2xMlcyODd6IN88US=> NX\CTVV7W0GQR1XS
KP-N-YS NXfKepZLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjJWGdKSzVyPUGuPVM2PTFizszN MoHhV2FPT0WU
DMS-153 NGC2fIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTFwOUe1PUDPxE1? M2fhOXNCVkeHUh?=
NB10 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fmbGlEPTB;Mj6yNFg4QCEQvF2= Mne2V2FPT0WU
NCI-H345 MoW4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvFZXVKSzVyPUKuOlE6ODVizszN MY\TRW5ITVJ?
SK-N-FI MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jEWWlEPTB;Mj63OVM5OSEQvF2= NVfiTIZDW0GQR1XS
NCI-H1092 NV3qNI5DT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYr1T2NvUUN3ME2yMlk2Ozl2IN88US=> MkfiV2FPT0WU
KM-H2 NVX6eow3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfTNnhKSzVyPUOuOlU{QTZizszN M2fKWXNCVkeHUh?=
KG-1 MlLXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTNwN{G2PFgh|ryP NELud2FUSU6JRWK=
D-283MED NV;yNHZGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIWzTIZKSzVyPUOuO|U5PyEQvF2= M4HZT3NCVkeHUh?=
LU-65 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWW0RZpMUUN3ME20MlEyPDh3IN88US=> NIH5RmVUSU6JRWK=
MSTO-211H MmTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnG5TWM2OD12LkG4PFk5KM7:TR?= M3\tZnNCVkeHUh?=
NCI-H2081 NEi5blZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{ezSWlEPTB;ND6yPVE4PCEQvF2= M1zrSnNCVkeHUh?=
D-247MG MlXWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPpTZdKSzVyPUSuOFMyQTFizszN M{e4[HNCVkeHUh?=
P31-FUJ M2OzRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDkPJdPUUN3ME20MlQ2ODF7IN88US=> NFjtVpVUSU6JRWK=
DMS-79 MkPPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTRwOUOxPFMh|ryP MkLBV2FPT0WU
KASUMI-1 M4nTNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\TbFFXUUN3ME20Mlk4PTB7IN88US=> NFfLXo5USU6JRWK=
JVM-3 NF\uWXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DORmlEPTB;NT6yOVAyPCEQvF2= NFPkNWpUSU6JRWK=
LAN-6 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1u0TmlEPTB;NT61NVc{PiEQvF2= M4LsZ3NCVkeHUh?=
DJM-1 M3\GW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLldI1KSzVyPUWuOVYxOjlizszN M2faOXNCVkeHUh?=
JVM-2 M3\1XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTVwNkewPVIh|ryP NHHw[ZZUSU6JRWK=
U-87-MG MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDNfYRQUUN3ME21Mlg6QTZ2IN88US=> MXnTRW5ITVJ?
SHP-77 M2jzTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPUTodyUUN3ME21Mlk1QTJ6IN88US=> MlLzV2FPT0WU
RPMI-8866 NUHxXIlLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTZwMkewNVMh|ryP NF31UmlUSU6JRWK=
ES7 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX72RmpHUUN3ME22MlM6QTJ2IN88US=> NG\DNVJUSU6JRWK=
WSU-NHL NVTtSG9OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1:2cGlEPTB;Nj61NVYxQSEQvF2= MV3TRW5ITVJ?
CAS-1 M3jGSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkTFTWM2OD15LkC5O|Q6KM7:TR?= M{Pp[3NCVkeHUh?=
MHH-NB-11 NFTBV|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fmNWlEPTB;OD6yN|E2OiEQvF2= M{fZbXNCVkeHUh?=
ES5 Mnm0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEflSmJKSzVyPUmuOVIzQDZizszN NELMSXNUSU6JRWK=
HDLM-2 MoGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHROodKSzVyPUmuPVU6ODJizszN M1HJ[nNCVkeHUh?=
NCI-H2171 NEOxRnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PXUmlEPTB;MUCuOVkzPyEQvF2= NICyWVRUSU6JRWK=
LNCaP-Clone-FGC Mme4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYO0NXlIUUN3ME2xNU4{PzF6IN88US=> MnjzV2FPT0WU
NCI-H1395 M{PvRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTF{LkK2PVkh|ryP MXLTRW5ITVJ?
NCI-H1436 M1TlN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIHmNmFKSzVyPUGzMlMyQTVizszN NF72eZBUSU6JRWK=
RCC10RGB M{PCPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorCTWM2OD1zMz6zNlIyKM7:TR?= M4DtZnNCVkeHUh?=
SW684 NVHtT495T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXkUG1iUUN3ME2xN{46Pzl5IN88US=> NVH6NGsxW0GQR1XS
NCI-H1650 NGrTflNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mme1TWM2OD1zNT6wOFQ4KM7:TR?= MorRV2FPT0WU
EW-13 M2TWbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFn1U2VKSzVyPUG1MlIxOjFizszN NXSyfWJiW0GQR1XS
EC-GI-10 NXf3SFZoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWX0XJRNUUN3ME2xOU45PjR2IN88US=> MoiyV2FPT0WU
CHP-126 NHnxbm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2q0R2lEPTB;MUeuNVkyQCEQvF2= NYfyZml1W0GQR1XS
NCI-H226 M1zFOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXKzTHd4UUN3ME2xPE4xPDB|IN88US=> NILRWpdUSU6JRWK=
NCI-H1838 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHwN3lsUUN3ME2yNE4yQDR6IN88US=> NYrZc5JQW0GQR1XS
EW-11 NGrwWHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2f4d2lEPTB;MkSuNlcyQCEQvF2= MYjTRW5ITVJ?
EB2 M{HQVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3\iRWlEPTB;M{GuOVMyQCEQvF2= NGDmOpRUSU6JRWK=
D-502MG MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULKelFYUUN3ME20N{46OjJ7IN88US=> M1zERnNCVkeHUh?=
U-698-M M3\Rfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXQXXk1UUN3ME20PE45PjdzIN88US=> NYPFNFJbW0GQR1XS
ES3 M{D2O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2KxTWlEPTB;NEmuN|g6OyEQvF2= MULTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol

Kinase Assay:[1]
- Collapse

mTORC1 and mTORC2 in Vitro Kinase Assays:

To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research:[1]
- Collapse
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: U87MG xenograft model
  • Formulation: Torin1 powder is first dissolved at 25 mg/mL in 100% N-methyl-2-pyrrolidone and subsequently diluted 1:4 with sterile 50% PEG400 to a final concentration of 5 mg/mL
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
20% NMP+50% PEG 400
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage powder
in solvent
Synonyms N/A

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Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    How to re-constitute the inhibitor for i.p. administration?

  • Answer:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

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