Torin 1

Catalog No.S2827

For research use only.

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

Torin 1 Chemical Structure

CAS No. 1222998-36-8

Selleck's Torin 1 has been cited by 174 publications

Purity & Quality Control

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Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
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2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 NXjGfHQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTBwMECwN|A3KM7:TR?= NEezOJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
OCUB-M MlfPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTBwMECxPVQh|ryP NUjR[JBERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
DOHH-2 M{O4WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPWSVdKSzVyPUCuNFAzOjVizszN NYnROXBLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
NOS-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDNTJY{UUN3ME2wMlAxOjR{IN88US=> Mm\uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
L-363 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\jW3VtUUN3ME2wMlAxOjh2IN88US=> M4nVeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
RL95-2 M3y3PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTBwMECzNkDPxE1? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
BL-70 MmjFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4f0T2lEPTB;MD6wNFM{PyEQvF2= M4DPVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
TGBC1TKB NWOxcFhpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7BTWM2OD1yLkCwN|c6KM7:TR?= Mm[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
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RPMI-8226 M3HkSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPxOYJnUUN3ME2wMlAxPjVzIN88US=> NYnwOJFGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
BL-41 M3\qXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTBwMEC2PVYh|ryP NH:1dGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
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EVSA-T Mof3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\ZSWdKSzVyPUCuNFA5QTJizszN NF3SU|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
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GCIY MnTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rHb2lEPTB;MD6wNVAxPyEQvF2= M2DtUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
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NALM-6 MoTGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTBwMEGxNFIh|ryP Ml:5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
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OPM-2 NXzvRo9FT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33qWmlEPTB;MD6wNVI6KM7:TR?= NVfNVGF4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
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KS-1 MnOyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LM[WlEPTB;MD6wNVM6OiEQvF2= MlTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
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BV-173 M4X1dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTBwMEKyNFMh|ryP NXHjSVkzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
SW872 NHv6R5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TDUGlEPTB;MD6wNlI1PyEQvF2= Mlr1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
SK-UT-1 NYC1flFRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;6OGlEPTB;MD6wNlI3OSEQvF2= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
OS-RC-2 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzPZohKSzVyPUCuNFI{OjNizszN MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
NCI-H747 M{DUd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHEfoRKSzVyPUCuNFI{OyEQvF2= NISzeY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
MZ2-MEL M1S3UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLoNmxEUUN3ME2wMlAzOzN5IN88US=> NUnlPY1NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
TE-11 NELlb5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHZdVRTUUN3ME2wMlAzOzd{IN88US=> Mor0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
KURAMOCHI NX\YVIFWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnywTWM2OD1yLkCyOFE1KM7:TR?= NXS1bG5IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
HD-MY-Z MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvZTWM2OD1yLkCyOFI3KM7:TR?= NEPsOIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
GI-1 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTBwMEK0Olkh|ryP NW\WXXpWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
LB1047-RCC NFHJRm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXkTWM2OD1yLkCyOFg5KM7:TR?= NVvWNY1iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
TE-12 MoHnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrTOFM{UUN3ME2wMlAzPTVizszN NH\SfZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
MZ7-mel M33zRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUK4Z|VMUUN3ME2wMlAzPjB7IN88US=> Mo[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
MMAC-SF MoLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrzU2g2UUN3ME2wMlAzPjN|IN88US=> NEHVcpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
A101D MmKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTBwMEK2OUDPxE1? MnP4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
GR-ST NGPabWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn:yTWM2OD1yLkCyOlU2KM7:TR?= NYfxeVRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
MC116 MoLFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHRTWM2OD1yLkCyOlgh|ryP NITh[2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
Ramos-2G6-4C10 NE\VXIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13vPWlEPTB;MD6wNlc2KM7:TR?= M{PQOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
ATN-1 MlPPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHT1VZJKSzVyPUCuNFI4QDlizszN NYroW3pvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
MFH-ino NYrUXHhST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHv4NmJKSzVyPUCuNFI5OTJizszN NIrkfZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
DEL NIHsPZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nxVWlEPTB;MD6wNlgyPSEQvF2= MlK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
OCI-AML2 NG\RbplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPPNoRKSzVyPUCuNFI5PzFizszN NEXVcVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
LXF-289 NUjqSXhQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlvsTWM2OD1yLkCyPFczKM7:TR?= NIP6XJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
IA-LM MoHBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwMEK5NVch|ryP M1LwPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
EoL-1-cell M4LK[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEjFfZlKSzVyPUCuNFI6QTZizszN NW\VO493RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
OVCAR-4 NU\l[2hWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PWO2lEPTB;MD6wN|A6PCEQvF2= NWL3cZY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
ES1 M3fkcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH[5XnNKSzVyPUCuNFMxQTdizszN M37lc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
CESS NYHacW5ZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLvbG1XUUN3ME2wMlA{ODl7IN88US=> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
GAK M4rsV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvIO4JGUUN3ME2wMlA{OTh4IN88US=> M4DtPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
NTERA-S-cl-D1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rJbGlEPTB;MD6wN|IzPiEQvF2= MmG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
TE-10 M1u0e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHCeoRKSzVyPUCuNFM{OTFizszN NWLQ[3BTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
CCRF-CEM NYe0S25TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEiyVJlKSzVyPUCuNFM{PzFizszN MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
NMC-G1 Mof2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXUTWM2OD1yLkCzOFQ5KM7:TR?= NWLNPJA1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
HOP-62 NUnBUHpFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3tTWM2OD1yLkCzOVU3KM7:TR?= NEHE[nk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
SH-4 NX64TWV5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPxTWM2OD1yLkCzOVgzKM7:TR?= NHq4XVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
NCI-H1648 NFP6VmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXMSZFPUUN3ME2wMlA{PjJ{IN88US=> NEfMdXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
EB-3 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPOUoNKSzVyPUCuNFM3PDNizszN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
BB65-RCC M3rqbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTBwMEO2PVEh|ryP MlG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
BE-13 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFTrSnNKSzVyPUCuNFM4PTlizszN NFHlSJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
CP67-MEL NH[zdm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTBwMEO3OVkh|ryP NEjpT|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
SW954 MlruS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;zWop6UUN3ME2wMlA{PzlizszN NEPo[WU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
TE-5 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkPGTWM2OD1yLkCzPFU1KM7:TR?= MnrnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
HCE-4 NEXC[4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3XvdmlEPTB;MD6wN|g2PSEQvF2= Mn;0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
LC-1F MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mle1TWM2OD1yLkCzPVg6KM7:TR?= M{WzR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
TK10 M1faOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3OWo45UUN3ME2wMlA1ODF7IN88US=> NWO2VXhMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
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Daudi NUnnflZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLTTWM2OD1yLkC0NlI2KM7:TR?= NFnrdGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
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Assay
Methods Test Index PMID
Western blot ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b ; LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236) ; p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1 ; Vinculin / p-S6 28494858 24126619 21965657 28757207
Immunofluorescence lipin-1 28347999
Growth inhibition assay Cell viability 28757207
In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol (from reference)

Kinase Assay:[1]
  • mTORC1 and mTORC2 in Vitro Kinase Assays:

    To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.

Cell Research:[1]
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
Animal Research:[2]
  • Animal Models: U87MG xenograft model
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How to re-constitute the inhibitor for i.p. administration?

Answer:
S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

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