Torin 1

Catalog No.S2827

Torin 1 Chemical Structure

Molecular Weight(MW): 607.62

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

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Cited by 16 Publications

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  • Huh-7.5 cells were treated with increasing concentration of Torin 1, a known autophagy inducer, and the autophagy response was assessed by measuring LC3II and p62 levels.

    Am J Pathol 2014 184(1), 214-29. Torin 1 purchased from Selleck.

  • Flag-tagged SENP3 was immunoprecipitated from HeLa cells and used for in vitro phosphorylation assay with [32P]ATP with either the mock-treated or Torin1-treated mTOR/Raptor/MLST8 complex. (Upper) The samples then were subjected to SDS-PAGE, followed by autoradiography. mTOR autophosphorylation is indicated by an asterisk. (Lower) Immunopurified SENP3 was monitored by Western blotting with anti-Flag antibody.

    Mol Cell Biol 2014 34(24), 4474-84. Torin 1 purchased from Selleck.

  • Representative Western blots showing LC3 in PP242 (PP) or Torin1 (Tor) treated HepG2 cells. HepG2 cells were treated with 10 uM PP242 or 0.1 uM Torin1 for 24 h.

    Biochem Biophys Res Commun 2014 10.1016/j.bbrc.2014.05.047. Torin 1 purchased from Selleck.

  • Bone marrow derived macrophages were pre-treated with 10nM Torin 1 for 1h prior to LPS treatment (100 ng/ml). TNF-a production was analyzed 24h later.

    Lim Hsiu Kim, Lina from National University of Singapore. Torin 1 purchased from Selleck.

  • Exp Cell Res, 2018, 363(1):84-94. Torin 1 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 NYPyV3NsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XadGlEPTB;MD6wNFA{ODZizszN MknXV2FPT0WU
OCUB-M NEnPRoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjKWJM1UUN3ME2wMlAxOTl2IN88US=> MnrkV2FPT0WU
DOHH-2 NGHsV5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTQXHBDUUN3ME2wMlAxOjJ3IN88US=> MV7TRW5ITVJ?
NOS-1 NEXsNI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTBwMECyOFIh|ryP MkfWV2FPT0WU
L-363 NXfoWYdyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEH5XFhKSzVyPUCuNFAzQDRizszN MUTTRW5ITVJ?
RL95-2 NIfYeXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2D1d2lEPTB;MD6wNFMzKM7:TR?= MX3TRW5ITVJ?
BL-70 M{DLWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkT1TWM2OD1yLkCwN|M4KM7:TR?= NY\HRm4xW0GQR1XS
TGBC1TKB MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPUXWZKSzVyPUCuNFA{PzlizszN MlXQV2FPT0WU
697 NVq0XYhMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXwOIE4UUN3ME2wMlAxPjN2IN88US=> M4K1TnNCVkeHUh?=
RPMI-8226 Mn[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwMEC2OVEh|ryP NVrhU3lGW0GQR1XS
BL-41 NHviT3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTBwMEC2PVYh|ryP NYnFPWN[W0GQR1XS
MRK-nu-1 M{nXb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoruTWM2OD1yLkCwO|A4KM7:TR?= NXr1ZWt4W0GQR1XS
EW-1 NFPWSmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvtdGxKSzVyPUCuNFA4PDlizszN NWHD[GJkW0GQR1XS
EVSA-T NYr0VWt3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlvRTWM2OD1yLkCwPFkzKM7:TR?= MnPWV2FPT0WU
HUTU-80 M4D6eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHkR2JzUUN3ME2wMlAxQTJ7IN88US=> MmTvV2FPT0WU
VA-ES-BJ NHrDOYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmm4TWM2OD1yLkCwPVg1KM7:TR?= M{frVHNCVkeHUh?=
GCIY NX7SO49mT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4e1fGlEPTB;MD6wNVAxPyEQvF2= M1jBXnNCVkeHUh?=
ES6 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXtTWM2OD1yLkCxNFg{KM7:TR?= NEH5T3dUSU6JRWK=
NALM-6 NH\LVHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXjTHZKSzVyPUCuNFEyODJizszN M3zGdnNCVkeHUh?=
LB996-RCC NYWxdIk4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LB[WlEPTB;MD6wNVE{PyEQvF2= MlK2V2FPT0WU
EW-16 M1nqWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXrTWM2OD1yLkCxNVkzKM7:TR?= NVXDe2FTW0GQR1XS
KALS-1 MlXjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTBwMEGxPVgh|ryP NIf6d4ZUSU6JRWK=
OPM-2 M3zxVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnyzTWM2OD1yLkCxNlkh|ryP MVjTRW5ITVJ?
KU812 M4PadWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTnOW1UUUN3ME2wMlAyOjl4IN88US=> MoDKV2FPT0WU
A4-Fuk MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTBwMEGzNVkh|ryP NVzj[XFYW0GQR1XS
KGN M1S3emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3ixXGlEPTB;MD6wNVM2QSEQvF2= MVfTRW5ITVJ?
KS-1 NIj1W5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknSTWM2OD1yLkCxN|kzKM7:TR?= MmXaV2FPT0WU
no-11 NHi4VoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFG1VGVKSzVyPUCuNFE{QTVizszN NYjrT|dYW0GQR1XS
PSN1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTBwMEG0NFkh|ryP NWqzeoZNW0GQR1XS
SK-LMS-1 NXPaT4lyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTBwMEG0NVMh|ryP MnnFV2FPT0WU
LS-411N M{j4Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPnUXZKSzVyPUCuNFE1OzlizszN MU\TRW5ITVJ?
HT-144 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV:1emU2UUN3ME2wMlAyPDVzIN88US=> NHvjUYRUSU6JRWK=
ACN M{LxfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4SwUmlEPTB;MD6wNVUh|ryP NVK2S|AyW0GQR1XS
RKO MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTBwMEG1N|Mh|ryP MV3TRW5ITVJ?
CMK MoHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkH0TWM2OD1yLkCxOVQ{KM7:TR?= M4rIdHNCVkeHUh?=
GB-1 NF:2VlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fF[mlEPTB;MD6wNVU2OyEQvF2= NEfmVWNUSU6JRWK=
KE-37 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mm\BTWM2OD1yLkCxOVc{KM7:TR?= M3jmV3NCVkeHUh?=
no-10 MkjMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHqTWM2OD1yLkCxOVg1KM7:TR?= NVLaU|NVW0GQR1XS
MOLT-16 NVHBOpp2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTBwMEG2NlIh|ryP NXv2dFVZW0GQR1XS
HEL NGPqUJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{CySWlEPTB;MD6wNVY1KM7:TR?= NEnWU4pUSU6JRWK=
NCI-H1355 M4\ZVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHmwfFRKSzVyPUCuNFE3PDZizszN MYDTRW5ITVJ?
IST-MEL1 NETzUIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TXUGlEPTB;MD6wNVc4PCEQvF2= NVn5b4gxW0GQR1XS
MHH-PREB-1 MkfNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjwcoxKSzVyPUCuNFE5OThizszN MUDTRW5ITVJ?
ES8 M{Ll[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTBwMEG4OFQh|ryP NIq1SoRUSU6JRWK=
LC-2-ad NX;qTXBPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlrYTWM2OD1yLkCyNVM4KM7:TR?= MU\TRW5ITVJ?
LAMA-84 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\3OWlEPTB;MD6wNlE3PyEQvF2= MW\TRW5ITVJ?
BV-173 NFHrU4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfRTG1KSzVyPUCuNFIzODNizszN MnjrV2FPT0WU
SW872 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPEPY5KSzVyPUCuNFIzPDdizszN MlrLV2FPT0WU
SK-UT-1 NWK1eZpLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vwWWlEPTB;MD6wNlI3OSEQvF2= MUfTRW5ITVJ?
OS-RC-2 Mn\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTBwMEKzNlMh|ryP MWDTRW5ITVJ?
NCI-H747 Mo\0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1yyW2lEPTB;MD6wNlM{KM7:TR?= NVr4[|lnW0GQR1XS
MZ2-MEL MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTBwMEKzN|ch|ryP NY[wUnJwW0GQR1XS
TE-11 MnfaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4m3RmlEPTB;MD6wNlM4OiEQvF2= MULTRW5ITVJ?
KURAMOCHI M1n0b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXjTWM2OD1yLkCyOFE1KM7:TR?= NHrIbYFUSU6JRWK=
HD-MY-Z NVj2U|BnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTBwMEK0NlYh|ryP MkS4V2FPT0WU
GI-1 MknqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;DfoFtUUN3ME2wMlAzPDZ7IN88US=> NUfRSHRZW0GQR1XS
LB1047-RCC M1zLU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTBwMEK0PFgh|ryP M3rCWnNCVkeHUh?=
TE-12 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTBwMEK1OUDPxE1? M2nBU3NCVkeHUh?=
MZ7-mel MoTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPjdlFPUUN3ME2wMlAzPjB7IN88US=> M2T1[3NCVkeHUh?=
MMAC-SF MoXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTBwMEK2N|Mh|ryP MoWzV2FPT0WU
A101D M4Xae2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHyNGk4UUN3ME2wMlAzPjVizszN Mne4V2FPT0WU
GR-ST NUTSZYpzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1u1bWlEPTB;MD6wNlY2PSEQvF2= NVTMbWk5W0GQR1XS
MC116 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnRc2NpUUN3ME2wMlAzPjhizszN M2j0eHNCVkeHUh?=
Ramos-2G6-4C10 MlfDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjYRVBnUUN3ME2wMlAzPzVizszN MmDoV2FPT0WU
ATN-1 M{DpdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXkU3VKSzVyPUCuNFI4QDlizszN MkDTV2FPT0WU
MFH-ino NYm5V2hyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXw[VFpUUN3ME2wMlAzQDF{IN88US=> MnXrV2FPT0WU
DEL MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vFS2lEPTB;MD6wNlgyPSEQvF2= M{jJ[XNCVkeHUh?=
OCI-AML2 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\KTWM2OD1yLkCyPFcyKM7:TR?= M{TYTXNCVkeHUh?=
LXF-289 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTBwMEK4O|Ih|ryP MoLQV2FPT0WU
IA-LM M1K1eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjkcY9rUUN3ME2wMlAzQTF5IN88US=> NFKzUlVUSU6JRWK=
EoL-1-cell M2nHc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTBwMEK5PVYh|ryP MkfLV2FPT0WU
OVCAR-4 NUHwNllsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjLWGxLUUN3ME2wMlA{ODl2IN88US=> M4jtTnNCVkeHUh?=
ES1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXDVWtKSzVyPUCuNFMxQTdizszN NUPPdm16W0GQR1XS
CESS NW\ISJhjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPscWFKSzVyPUCuNFMxQTlizszN MVfTRW5ITVJ?
GAK M322[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXWbGRNUUN3ME2wMlA{OTh4IN88US=> M2LJdXNCVkeHUh?=
NTERA-S-cl-D1 NUi2XJBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLrd2dRUUN3ME2wMlA{OjJ4IN88US=> MkD2V2FPT0WU
TE-10 NFLaXVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLJdGNKSzVyPUCuNFM{OTFizszN NHfQeY1USU6JRWK=
CCRF-CEM NHi5codIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnhbpNKSzVyPUCuNFM{PzFizszN NFnubmlUSU6JRWK=
NMC-G1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfVblJKSzVyPUCuNFM1PDhizszN NXK5WY86W0GQR1XS
HOP-62 NUPVcWFxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHWTWM2OD1yLkCzOVU3KM7:TR?= NWXI[2ViW0GQR1XS
SH-4 MnvsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTBwMEO1PFIh|ryP NGmzcVFUSU6JRWK=
NCI-H1648 M2nqTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmqxTWM2OD1yLkCzOlIzKM7:TR?= MYfTRW5ITVJ?
EB-3 M4LBR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2H4W2lEPTB;MD6wN|Y1OyEQvF2= MkC2V2FPT0WU
BB65-RCC MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHw[4d[UUN3ME2wMlA{PjlzIN88US=> MnHEV2FPT0WU
BE-13 M37xXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTrdIszUUN3ME2wMlA{PzV7IN88US=> M{TlNHNCVkeHUh?=
CP67-MEL MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1HyNmlEPTB;MD6wN|c2QSEQvF2= MXXTRW5ITVJ?
SW954 NFPXWIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3O1WWlEPTB;MD6wN|c6KM7:TR?= MlP3V2FPT0WU
TE-5 MkjzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\ae4VKSzVyPUCuNFM5PTRizszN MlWyV2FPT0WU
HCE-4 MmP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7ybGdjUUN3ME2wMlA{QDV3IN88US=> MkjwV2FPT0WU
LC-1F MkG5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3q0RWlEPTB;MD6wN|k5QSEQvF2= M4e2O3NCVkeHUh?=
TK10 M3TocWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTBwMESwNVkh|ryP MVTTRW5ITVJ?
SJSA-1 M{XJSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jFTmlEPTB;MD6wOFE5QCEQvF2= MX\TRW5ITVJ?
Daudi MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnLTWM2OD1yLkC0NlI2KM7:TR?= NX35N4htW0GQR1XS
SW982 NYTC[mp5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX63XndYUUN3ME2wMlA1OjR4IN88US=> MnLxV2FPT0WU
SF126 MlnSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTBwMESzOlEh|ryP MXrTRW5ITVJ?
TE-15 M2nZWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LFTGlEPTB;MD6wOFQ{PSEQvF2= NUfaPIFHW0GQR1XS
COLO-684 M37HcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTBwMES1OlEh|ryP MUDTRW5ITVJ?
8-MG-BA NEXuempIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\PeItKSzVyPUCuNFQ3OjlizszN MoXFV2FPT0WU
NB13 Mlr6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTBTWM2OD1yLkC0O|c3KM7:TR?= NH3RUGlUSU6JRWK=
HCC1599 M2PmOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYj0[JBLUUN3ME2wMlA1QDd7IN88US=> MknRV2FPT0WU
SK-PN-DW M{K1Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPkb3BDUUN3ME2wMlA1QTR7IN88US=> M3PyZXNCVkeHUh?=
ALL-PO M4Lk[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTBwMES5OVYh|ryP MU\TRW5ITVJ?
NB6 MnTNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrpdlhKSzVyPUCuNFUzPzFizszN M1TrPHNCVkeHUh?=
IST-SL2 NIrUSoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mkf5TWM2OD1yLkC1N|YzKM7:TR?= NFfnfnJUSU6JRWK=
BB30-HNC NYexUWhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTR[2hKSzVyPUCuNFU4OyEQvF2= MmfJV2FPT0WU
SW962 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XyT2lEPTB;MD6wOVc3OyEQvF2= MlTyV2FPT0WU
TGBC24TKB MmXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4C0UGlEPTB;MD6wOVc6QSEQvF2= NFyxTJJUSU6JRWK=
KINGS-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTBwMEW4NFMh|ryP M4XaSXNCVkeHUh?=
HC-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PqeGlEPTB;MD6wOVgzPyEQvF2= NIXOW2RUSU6JRWK=
RS4-11 NUXVc3JDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTBwMEW5N|gh|ryP MofyV2FPT0WU
LS-123 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTBwMEW5OFIh|ryP NX7UVml3W0GQR1XS
A3-KAW NV\HcG1sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTBwME[yNlUh|ryP Mn\mV2FPT0WU
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NOMO-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIrpR2tKSzVyPUCuN|k6PzFizszN M2jFPXNCVkeHUh?=
MS-1 NVrnS|h{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV3VWHZCUUN3ME2wMlQxOTd5IN88US=> Mn74V2FPT0WU
EKVX NXq5U|hVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3OxbWlEPTB;MD60OFQ3OSEQvF2= NHrnfllUSU6JRWK=
SK-MEL-2 Mo[2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjxcFhKSzVyPUCuOFY1KM7:TR?= NVfyTlFjW0GQR1XS
HCC1187 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPMTWM2OD1yLkS2O|Y5KM7:TR?= MVPTRW5ITVJ?
HCC2218 NFnlT3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTBwNEeyNlch|ryP M1XufXNCVkeHUh?=
NCI-H187 MkK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTBwNEe4OEDPxE1? M{X5dnNCVkeHUh?=
DB NX:3RWJjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TWcGlEPTB;MD61NFA2PSEQvF2= MnXRV2FPT0WU
NCI-H69 Mn3aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwNUCyOFch|ryP MlSyV2FPT0WU
NCI-H1694 M3\wSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4X5WmlEPTB;MD61NFY6PyEQvF2= Ml3KV2FPT0WU
CA46 M{jVSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWfJR|UxRTBwNUC5NVch|ryP M4\idHNCVkeHUh?=
EHEB MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;GTWM2OD1yLkWxNVk{KM7:TR?= MmXXV2FPT0WU
NCCIT MnvpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DDW2lEPTB;MD61OFMzOSEQvF2= MkTZV2FPT0WU
NCI-H720 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzoTWM2OD1yLkW0PVYzKM7:TR?= M4\IZnNCVkeHUh?=
LU-134-A NV7OVJZxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nOOGlEPTB;MD61OVI6PiEQvF2= MkTzV2FPT0WU
MC-CAR NFf2OJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTBwNUW0N|Eh|ryP NYi5S4l4W0GQR1XS
NEC8 M2\jSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LWXWlEPTB;MD61OlE4OiEQvF2= MYfTRW5ITVJ?
NCI-H524 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXTeGJxUUN3ME2wMlU5PzR6IN88US=> M3rhbXNCVkeHUh?=
NCI-H1963 NVrx[nM{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;Eb2lEPTB;MD62NlU5KM7:TR?= M3nTNHNCVkeHUh?=
SU-DHL-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXESXFbUUN3ME2wMlY1PTJ4IN88US=> M2[3d3NCVkeHUh?=
NCI-H1522 NIfF[HpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fpe2lEPTB;MD62OFg5OyEQvF2= M37NfnNCVkeHUh?=
NCI-H1155 NIDSd2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX[3RoVJUUN3ME2wMlY2OzVizszN M1fYd3NCVkeHUh?=
D-263MG NX3rVFZNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLFTGJKSzVyPUCuOlYyODFizszN NHjJfG1USU6JRWK=
TE-6 MmThS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDFTFZKSzVyPUCuOlgyODRizszN Ml;rV2FPT0WU
NCI-H1417 M1LhNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTBwNkm3NVEh|ryP M{O4NnNCVkeHUh?=
NCI-H510A MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPQRXFJUUN3ME2wMlc4OjN4IN88US=> NEHmRYxUSU6JRWK=
KARPAS-45 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDje3JKSzVyPUCuO|gzOzVizszN NHXMZnJUSU6JRWK=
IST-SL1 M3vLN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlG1TWM2OD1yLki1PFI2KM7:TR?= NY\SdFNHW0GQR1XS
COLO-824 NGGwdYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPNdIJjUUN3ME2wMlg4QDN3IN88US=> MWXTRW5ITVJ?
NCI-H526 MmLMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTBwOUC5OVgh|ryP M33uSXNCVkeHUh?=
NCI-H719 MoXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnrBTWM2OD1yLkm2OlU4KM7:TR?= MU\TRW5ITVJ?
SF539 M2\xVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXLWYFUUUN3ME2wMlk5Ozl4IN88US=> MWPTRW5ITVJ?
NKM-1 NGfWPYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTDTWM2OD1yLkm4OlY{KM7:TR?= NVfUTppZW0GQR1XS
A253 MojDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jZXGlEPTB;MD65PFg5PSEQvF2= NIrnd|NUSU6JRWK=
TE-441-T MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nnbGlEPTB;MT6wOFM2KM7:TR?= MkK3V2FPT0WU
COR-L88 NELvbmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NET3dYlKSzVyPUGuNVE{PTRizszN NVPqeYVEW0GQR1XS
CPC-N NY\heJpXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfxb5lKSzVyPUGuNVU5QDhizszN M2rpRnNCVkeHUh?=
NCI-H2107 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3flTGlEPTB;MT6xO|I3OSEQvF2= MkjTV2FPT0WU
ECC4 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkW2TWM2OD1zLkG4Olk{KM7:TR?= M2[3dXNCVkeHUh?=
NCI-H2141 MmrHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jVfGlEPTB;MT6xPVI6QSEQvF2= MXrTRW5ITVJ?
NCI-H1770 MlSyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTFwM{K5Nlgh|ryP M1PmU3NCVkeHUh?=
NCI-SNU-1 Mk\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTFwM{OwOEDPxE1? NIS4OGtUSU6JRWK=
LU-165 M3zldWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TmZ2lEPTB;MT60OVQ3PyEQvF2= MYjTRW5ITVJ?
Raji MkPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTFwNEeyOVkh|ryP MV;TRW5ITVJ?
EW-24 MorCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrVcG5KSzVyPUGuOVQ3OTlizszN NXTvR2JJW0GQR1XS
KP-N-RT-BM-1 Mo\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfhUWdDUUN3ME2xMlU3PTVzIN88US=> NX;BbI0{W0GQR1XS
SK-MEL-1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTFwNkS4O|ch|ryP M1zrR3NCVkeHUh?=
SCLC-21H NYHrNYEzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vSUGlEPTB;MT62OlY2OSEQvF2= NHPpWZdUSU6JRWK=
SIMA Ml;tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jLeGlEPTB;MT62PFI{OyEQvF2= NEjpXVlUSU6JRWK=
NCI-H1304 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTFwN{C3OlUh|ryP NITh[|ZUSU6JRWK=
REH MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\nVXBFUUN3ME2xMlcyODd6IN88US=> NV;peIJsW0GQR1XS
KP-N-YS M2fHemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDZTWM2OD1zLkmzOVUyKM7:TR?= MU\TRW5ITVJ?
DMS-153 NWnyTVg5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{CyfGlEPTB;MT65O|U6KM7:TR?= M3roeHNCVkeHUh?=
NB10 M{jMfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTJwMkC4O|gh|ryP Mn7rV2FPT0WU
NCI-H345 MlX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2q4cWlEPTB;Mj62NVkxPSEQvF2= NVXxfVBKW0GQR1XS
SK-N-FI NWDlWphHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTJwN{WzPFEh|ryP MWjTRW5ITVJ?
NCI-H1092 NI[wfZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4i5WGlEPTB;Mj65OVM6PCEQvF2= MVTTRW5ITVJ?
KM-H2 NVrn[IF[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TO[2lEPTB;Mz62OVM6PiEQvF2= M2PWTnNCVkeHUh?=
KG-1 MoixS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXnTWM2OD1|LkexOlg5KM7:TR?= M1zqZXNCVkeHUh?=
D-283MED M4DneWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnS3TWM2OD1|Lke1PFch|ryP MmOzV2FPT0WU
LU-65 NEjW[HJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLO[JNKSzVyPUSuNVE1QDVizszN MXPTRW5ITVJ?
MSTO-211H NVHKdYI6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnyTWM2OD12LkG4PFk5KM7:TR?= NUnsUWRCW0GQR1XS
NCI-H2081 M2n0N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1W3TmlEPTB;ND6yPVE4PCEQvF2= MXHTRW5ITVJ?
D-247MG NYLEV4Y6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\6TWM2OD12LkSzNVkyKM7:TR?= MkjLV2FPT0WU
P31-FUJ M4LkT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LQbWlEPTB;ND60OVAyQSEQvF2= NXO5TVR1W0GQR1XS
DMS-79 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzRe45{UUN3ME20Mlk{OTh|IN88US=> NHuxcXdUSU6JRWK=
KASUMI-1 Mk[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTRwOUe1NFkh|ryP NX\IXGlNW0GQR1XS
JVM-3 NFjybmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorKTWM2OD13LkK1NFE1KM7:TR?= NF\BWIFUSU6JRWK=
LAN-6 M3j3T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4r5eGlEPTB;NT61NVc{PiEQvF2= MVXTRW5ITVJ?
DJM-1 M1;wcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rUV2lEPTB;NT61OlAzQSEQvF2= M1Hi[nNCVkeHUh?=
JVM-2 NGPkU2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTnepIxUUN3ME21MlY4ODl{IN88US=> M2DKO3NCVkeHUh?=
U-87-MG MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTVwOEm5OlQh|ryP M4\EPHNCVkeHUh?=
SHP-77 MljoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jqeWlEPTB;NT65OFkzQCEQvF2= NHXjSFVUSU6JRWK=
RPMI-8866 NFjRdpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorITWM2OD14LkK3NFE{KM7:TR?= M3T2RnNCVkeHUh?=
ES7 NF;MXpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfPNVJJUUN3ME22MlM6QTJ2IN88US=> M37j[HNCVkeHUh?=
WSU-NHL NYnPO2RxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHoTWM2OD14LkWxOlA6KM7:TR?= NX3UfYQ2W0GQR1XS
CAS-1 MnXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|UxRTdwMEm3OFkh|ryP M{LwNXNCVkeHUh?=
MHH-NB-11 M3\CWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M165emlEPTB;OD6yN|E2OiEQvF2= Ml;vV2FPT0WU
ES5 NHLacnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zIW2lEPTB;OT61NlI5PiEQvF2= MmDTV2FPT0WU
HDLM-2 NEnm[WlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrUTWM2OD17Lkm1PVAzKM7:TR?= NVHqUnliW0GQR1XS
NCI-H2171 NVj6OHo6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPw[VRCUUN3ME2xNE42QTJ5IN88US=> MYnTRW5ITVJ?
LNCaP-Clone-FGC NUXHPZl1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\sTWM2OD1zMT6zO|E5KM7:TR?= M{f4eHNCVkeHUh?=
NCI-H1395 M3;jWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvlVldKSzVyPUGyMlI3QTlizszN M{DoUnNCVkeHUh?=
NCI-H1436 NIXyfFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzVfJdKSzVyPUGzMlMyQTVizszN Mn7iV2FPT0WU
RCC10RGB M334dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLPT4ZOUUN3ME2xN{4{OjJzIN88US=> NXfwPYc1W0GQR1XS
SW684 M4qyUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTF|Lkm3PVch|ryP NHP6OIhUSU6JRWK=
NCI-H1650 NEnPRmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\nOmlEPTB;MUWuNFQ1PyEQvF2= Ml\qV2FPT0WU
EW-13 NEL6OYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYKxNmNnUUN3ME2xOU4zODJzIN88US=> M3u1RnNCVkeHUh?=
EC-GI-10 MnvLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\UTWM2OD1zNT64OlQ1KM7:TR?= NX3XUHFDW0GQR1XS
CHP-126 NH\3TZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3P4XmlEPTB;MUeuNVkyQCEQvF2= NHLFNZVUSU6JRWK=
NCI-H226 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTF6LkC0NFMh|ryP MkT6V2FPT0WU
NCI-H1838 NHrKZlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTJyLkG4OFgh|ryP NUPzVG9PW0GQR1XS
EW-11 NVXwfFRiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYL2OJUyUUN3ME2yOE4zPzF6IN88US=> Mni5V2FPT0WU
EB2 M3LS[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrPT5lJUUN3ME2zNU42OzF6IN88US=> M4XlO3NCVkeHUh?=
D-502MG M1K5dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfnTWM2OD12Mz65NlI6KM7:TR?= M4HjfXNCVkeHUh?=
U-698-M NXHRVXhPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGD0SIJKSzVyPUS4Mlg3PzFizszN MoPmV2FPT0WU
ES3 NGPV[4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fhW2lEPTB;NEmuN|g6OyEQvF2= MX7TRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol

Kinase Assay:[1]
+ Expand

mTORC1 and mTORC2 in Vitro Kinase Assays:

To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research:[1]
+ Expand
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
    (Only for Reference)
Animal Research:[2]
+ Expand
  • Animal Models: U87MG xenograft model
  • Formulation: Torin1 powder is first dissolved at 25 mg/mL in 100% N-methyl-2-pyrrolidone and subsequently diluted 1:4 with sterile 50% PEG400 to a final concentration of 5 mg/mL
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
20% NMP+50% PEG 400
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage powder
in solvent
Synonyms N/A

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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to re-constitute the inhibitor for i.p. administration?

  • Answer:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID