Torin 1

For research use only.

Catalog No.S2827

119 publications

Torin 1 Chemical Structure

CAS No. 1222998-36-8

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

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Selleck's Torin 1 has been cited by 119 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 NXHXPFlRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\3SWlEPTB;MD6wNFA{ODZizszN M{HBTXNCVkeHUh?=
OCUB-M MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlG5TWM2OD1yLkCwNVk1KM7:TR?= M{PaWnNCVkeHUh?=
DOHH-2 MmXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3YTWM2OD1yLkCwNlI2KM7:TR?= NIfp[XFUSU6JRWK=
NOS-1 M1K5[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDheW52UUN3ME2wMlAxOjR{IN88US=> NFHDOFdUSU6JRWK=
L-363 NVP1WXVOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnXETWM2OD1yLkCwNlg1KM7:TR?= MnPTV2FPT0WU
RL95-2 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrFfZFEUUN3ME2wMlAxOzJizszN NIfpN|FUSU6JRWK=
BL-70 MnTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEm3OFBKSzVyPUCuNFA{OzdizszN Mn;FV2FPT0WU
TGBC1TKB M1TKXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH2xWXhKSzVyPUCuNFA{PzlizszN Mo\vV2FPT0WU
697 NHzDfoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{D2VmlEPTB;MD6wNFY{PCEQvF2= MnjLV2FPT0WU
RPMI-8226 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTBwMEC2OVEh|ryP NUHlTnlRW0GQR1XS
BL-41 MmDHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTBwMEC2PVYh|ryP NW\keXI4W0GQR1XS
MRK-nu-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPNb5k{UUN3ME2wMlAxPzB5IN88US=> NHvGN3pUSU6JRWK=
EW-1 NYDoNIRQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mkf4TWM2OD1yLkCwO|Q6KM7:TR?= MWDTRW5ITVJ?
EVSA-T M4KwTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY[1b3Q1UUN3ME2wMlAxQDl{IN88US=> NVLQeFZrW0GQR1XS
HUTU-80 MkGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXO3clR{UUN3ME2wMlAxQTJ7IN88US=> MkfjV2FPT0WU
VA-ES-BJ MkezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTBwMEC5PFQh|ryP MXrTRW5ITVJ?
GCIY NV3OVoRTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\HWWFZUUN3ME2wMlAyODB5IN88US=> MnrKV2FPT0WU
ES6 Mli3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;UPGlEPTB;MD6wNVA5OyEQvF2= M4DCUXNCVkeHUh?=
NALM-6 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTBwMEGxNFIh|ryP NETBfYdUSU6JRWK=
LB996-RCC M3:1UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoP6TWM2OD1yLkCxNVM4KM7:TR?= NIPRNWVUSU6JRWK=
EW-16 NYP1WIZzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XPcGlEPTB;MD6wNVE6OiEQvF2= M3zqenNCVkeHUh?=
KALS-1 NFLQeYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTBwMEGxPVgh|ryP NH;PPYpUSU6JRWK=
OPM-2 Mnj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\wTWM2OD1yLkCxNlkh|ryP NUjBeG1rW0GQR1XS
KU812 NXjtWmxHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XqRmlEPTB;MD6wNVI6PiEQvF2= NEewW5dUSU6JRWK=
A4-Fuk NEfEVIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDQc4dEUUN3ME2wMlAyOzF7IN88US=> NXTWWmo2W0GQR1XS
KGN MlSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzzTWM2OD1yLkCxN|U6KM7:TR?= M1jJb3NCVkeHUh?=
KS-1 NYPKSFh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTBwMEGzPVIh|ryP MnH6V2FPT0WU
no-11 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvk[VBKSzVyPUCuNFE{QTVizszN NXXkflB3W0GQR1XS
PSN1 Mlz5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvlOnNKSzVyPUCuNFE1ODlizszN NYHLZVFxW0GQR1XS
SK-LMS-1 NYXvUZpJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nDSGlEPTB;MD6wNVQyOyEQvF2= M1PzeHNCVkeHUh?=
LS-411N MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{POeWlEPTB;MD6wNVQ{QSEQvF2= NU\Dfm5SW0GQR1XS
HT-144 NVq5SJhLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTBwMEG0OVEh|ryP Ml[wV2FPT0WU
ACN M{XN[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHlTWM2OD1yLkCxOUDPxE1? MkLCV2FPT0WU
RKO MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\ZNG1KSzVyPUCuNFE2OzNizszN NHTNSJVUSU6JRWK=
CMK MkPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTCTWM2OD1yLkCxOVQ{KM7:TR?= M3nBVXNCVkeHUh?=
GB-1 NF3nPINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XQVWlEPTB;MD6wNVU2OyEQvF2= NXHYeJVbW0GQR1XS
KE-37 MlH4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTBwMEG1O|Mh|ryP MnvCV2FPT0WU
no-10 M{n6cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTBwMEG1PFQh|ryP MWfTRW5ITVJ?
MOLT-16 NXL6[5pHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LIWWlEPTB;MD6wNVYzOiEQvF2= NEi5emhUSU6JRWK=
HEL NIOwXZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFuzWllKSzVyPUCuNFE3PCEQvF2= NY\6Z5JMW0GQR1XS
NCI-H1355 Ml\iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLDc|NKSzVyPUCuNFE3PDZizszN Mnv2V2FPT0WU
IST-MEL1 NEHmUGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXH4dXVjUUN3ME2wMlAyPzd2IN88US=> MWXTRW5ITVJ?
MHH-PREB-1 NEPzVIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTBwMEG4NVgh|ryP M2ThWnNCVkeHUh?=
ES8 M1\sRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELDbmJKSzVyPUCuNFE5PDRizszN MU\TRW5ITVJ?
LC-2-ad NITGXm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnhXYZKSzVyPUCuNFIyOzdizszN M2Hw[XNCVkeHUh?=
LAMA-84 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\BbWlEPTB;MD6wNlE3PyEQvF2= NXjWRnQ5W0GQR1XS
BV-173 NX;4VJFTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMEKyNFMh|ryP NVjD[4tmW0GQR1XS
SW872 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGXJSZdKSzVyPUCuNFIzPDdizszN MWHTRW5ITVJ?
SK-UT-1 NX7mUVdFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrSfFNjUUN3ME2wMlAzOjZzIN88US=> NFTvSYhUSU6JRWK=
OS-RC-2 Mof6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkH1TWM2OD1yLkCyN|I{KM7:TR?= NHz3PIVUSU6JRWK=
NCI-H747 MmrwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrxTWM2OD1yLkCyN|Mh|ryP NHv0OlhUSU6JRWK=
MZ2-MEL M2\5c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnJNW1KSzVyPUCuNFI{OzdizszN NViwR4ppW0GQR1XS
TE-11 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTBwMEKzO|Ih|ryP MmT1V2FPT0WU
KURAMOCHI MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7DbGdTUUN3ME2wMlAzPDF2IN88US=> M2L1R3NCVkeHUh?=
HD-MY-Z MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGi1e2NKSzVyPUCuNFI1OjZizszN NH\W[Y9USU6JRWK=
GI-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTBwMEK0Olkh|ryP NGTJPXdUSU6JRWK=
LB1047-RCC NYH2d5NMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPWbnJjUUN3ME2wMlAzPDh6IN88US=> Mn:2V2FPT0WU
TE-12 M{GwO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13DW2lEPTB;MD6wNlU2KM7:TR?= M{frSHNCVkeHUh?=
MZ7-mel NGTpOVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzqUYZKSzVyPUCuNFI3ODlizszN NIfoSYpUSU6JRWK=
MMAC-SF M{DnU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkTJTWM2OD1yLkCyOlM{KM7:TR?= M3XGTHNCVkeHUh?=
A101D MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jYbmlEPTB;MD6wNlY2KM7:TR?= NWDMc4ZTW0GQR1XS
GR-ST M2XRRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTBwMEK2OVUh|ryP MV\TRW5ITVJ?
MC116 M3rWW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\DcmlEPTB;MD6wNlY5KM7:TR?= M3\FbXNCVkeHUh?=
Ramos-2G6-4C10 MnLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTBwMEK3OUDPxE1? MnHEV2FPT0WU
ATN-1 NXTrfY5OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoGxTWM2OD1yLkCyO|g6KM7:TR?= MWDTRW5ITVJ?
MFH-ino NGDUT2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2ix[mlEPTB;MD6wNlgyOiEQvF2= MWjTRW5ITVJ?
DEL MnLNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPOXXJLUUN3ME2wMlAzQDF3IN88US=> NXW4OXpMW0GQR1XS
OCI-AML2 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mmf1TWM2OD1yLkCyPFcyKM7:TR?= MWfTRW5ITVJ?
LXF-289 Moi4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HNfWlEPTB;MD6wNlg4OiEQvF2= NYSyb2huW0GQR1XS
IA-LM M4XqeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mlz4TWM2OD1yLkCyPVE4KM7:TR?= MVzTRW5ITVJ?
EoL-1-cell Mn;4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\OZoxKSzVyPUCuNFI6QTZizszN M1\G[nNCVkeHUh?=
OVCAR-4 NH\KV3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTBwMEOwPVQh|ryP MkDSV2FPT0WU
ES1 MmHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTBwMEOwPVch|ryP MnXXV2FPT0WU
CESS M2T1TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTBwMEOwPVkh|ryP NF;R[GxUSU6JRWK=
GAK Ml\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFjEVXlKSzVyPUCuNFMyQDZizszN M3fQWXNCVkeHUh?=
NTERA-S-cl-D1 NHzyd2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTBwMEOyNlYh|ryP NV;hPZRmW0GQR1XS
TE-10 MmqwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjTTWM2OD1yLkCzN|EyKM7:TR?= MVrTRW5ITVJ?
CCRF-CEM NGHOXXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jVTGlEPTB;MD6wN|M4OSEQvF2= NEjTfFRUSU6JRWK=
NMC-G1 NYrFdJdFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUe1c|JPUUN3ME2wMlA{PDR6IN88US=> M3zFe3NCVkeHUh?=
HOP-62 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELobGVKSzVyPUCuNFM2PTZizszN MnzQV2FPT0WU
SH-4 NH31ZZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfncXJPUUN3ME2wMlA{PTh{IN88US=> MmflV2FPT0WU
NCI-H1648 MnvpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTBwMEO2NlIh|ryP NUDMPHBtW0GQR1XS
EB-3 M3yxbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn36TWM2OD1yLkCzOlQ{KM7:TR?= NXHtb3RuW0GQR1XS
BB65-RCC M1LQ[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvPTWM2OD1yLkCzOlkyKM7:TR?= MlO3V2FPT0WU
BE-13 M3X2SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTBwMEO3OVkh|ryP NEXVWmVUSU6JRWK=
CP67-MEL NYm4RnV5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTBwMEO3OVkh|ryP MUPTRW5ITVJ?
SW954 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLxbJV2UUN3ME2wMlA{PzlizszN NFnkXXdUSU6JRWK=
TE-5 NU\WWVBUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfZV2JKSzVyPUCuNFM5PTRizszN MV3TRW5ITVJ?
HCE-4 NITmNXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHUTWM2OD1yLkCzPFU2KM7:TR?= Mn7pV2FPT0WU
LC-1F MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHz1UppKSzVyPUCuNFM6QDlizszN MU\TRW5ITVJ?
TK10 NWjVcHdHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnYUmNwUUN3ME2wMlA1ODF7IN88US=> MkXhV2FPT0WU
SJSA-1 NUC0SVVkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn64TWM2OD1yLkC0NVg5KM7:TR?= M2HRVHNCVkeHUh?=
Daudi MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7JTo9KUUN3ME2wMlA1OjJ3IN88US=> NUjqZWlTW0GQR1XS
SW982 MnXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknOTWM2OD1yLkC0NlQ3KM7:TR?= MUTTRW5ITVJ?
SF126 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\5[GlEPTB;MD6wOFM3OSEQvF2= NHHkNoJUSU6JRWK=
TE-15 M{e4WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHoUWtKSzVyPUCuNFQ1OzVizszN NHzVbpZUSU6JRWK=
COLO-684 NIflW4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEGxVGdKSzVyPUCuNFQ2PjFizszN NH7SOoZUSU6JRWK=
8-MG-BA MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDMeWdlUUN3ME2wMlA1PjJ7IN88US=> MYXTRW5ITVJ?
NB13 MknhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1O2ZWlEPTB;MD6wOFc4PiEQvF2= NEDwc2JUSU6JRWK=
HCC1599 NX;kZ256T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7wZ5RqUUN3ME2wMlA1QDd7IN88US=> M1rzTnNCVkeHUh?=
SK-PN-DW MkHDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPNTWM2OD1yLkC0PVQ6KM7:TR?= MYDTRW5ITVJ?
ALL-PO NXv4WmQyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4rp[WlEPTB;MD6wOFk2PiEQvF2= MVLTRW5ITVJ?
NB6 Mm\qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1fsVmlEPTB;MD6wOVI4OSEQvF2= MlLkV2FPT0WU
IST-SL2 Mn\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzITWM2OD1yLkC1N|YzKM7:TR?= NXPZfIk6W0GQR1XS
BB30-HNC MnjhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTBwMEW3N{DPxE1? M{LjNHNCVkeHUh?=
SW962 NGTuR3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVrld4dVUUN3ME2wMlA2PzZ|IN88US=> NYS4e5RyW0GQR1XS
TGBC24TKB M1jqW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXTN|ZKSzVyPUCuNFU4QTlizszN MUjTRW5ITVJ?
KINGS-1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnqTWM2OD1yLkC1PFA{KM7:TR?= Ml7FV2FPT0WU
HC-1 M1K0S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWO3RY9CUUN3ME2wMlA2QDJ5IN88US=> MUXTRW5ITVJ?
RS4-11 MorlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTBwMEW5N|gh|ryP NW\KdY85W0GQR1XS
LS-123 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIf2VGpKSzVyPUCuNFU6PDJizszN M4DGbHNCVkeHUh?=
A3-KAW MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Dhc2lEPTB;MD6wOlIzPSEQvF2= M33zSHNCVkeHUh?=
SNB75 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nxcmlEPTB;MD6wOlM1OSEQvF2= MnKxV2FPT0WU
SR M1zW[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITqdlNKSzVyPUCuNFY{PzRizszN NYHSS45XW0GQR1XS
LOUCY NH;OS21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\jUZJKSzVyPUCuNFY{QTRizszN M4\0dHNCVkeHUh?=
K5 M{[2VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\iTWM2OD1yLkC2OFA{KM7:TR?= Ml\QV2FPT0WU
Becker M4XGd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LBUWlEPTB;MD6wOlQzKM7:TR?= MV3TRW5ITVJ?
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NCI-H187 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXETWM2OD1yLkS3PFQh|ryP NWPyXGF[W0GQR1XS
DB NWnjTGR1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\LSW1{UUN3ME2wMlUxODV3IN88US=> M{[xSHNCVkeHUh?=
NCI-H69 M2L5UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTBwNUCyOFch|ryP MXLTRW5ITVJ?
NCI-H1694 NGTwPJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTBwNUC2PVch|ryP MUnTRW5ITVJ?
CA46 NUL5cYtHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmH5TWM2OD1yLkWwPVE4KM7:TR?= MnnYV2FPT0WU
EHEB Mmm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTBwNUGxPVMh|ryP NWTUfZBIW0GQR1XS
NCCIT NHHGfmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPjTWM2OD1yLkW0N|IyKM7:TR?= MVTTRW5ITVJ?
NCI-H720 MkPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUC3R4J1UUN3ME2wMlU1QTZ{IN88US=> MX7TRW5ITVJ?
LU-134-A Mnu1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\EZ2lEPTB;MD61OVI6PiEQvF2= MXLTRW5ITVJ?
MC-CAR MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfXVpFuUUN3ME2wMlU2PDNzIN88US=> NIrVN25USU6JRWK=
NEC8 MmrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYn5VYFrUUN3ME2wMlU3OTd{IN88US=> M2nmeHNCVkeHUh?=
NCI-H524 NWX5W5RST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIf6dYxKSzVyPUCuOVg4PDhizszN NX;JSY9{W0GQR1XS
NCI-H1963 MmW5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fxd2lEPTB;MD62NlU5KM7:TR?= NWC4SGtkW0GQR1XS
SU-DHL-1 NV3wcG9zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfv[2lKSzVyPUCuOlQ2OjZizszN M37YS3NCVkeHUh?=
NCI-H1522 M3nSdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnUTWM2OD1yLk[0PFg{KM7:TR?= MUHTRW5ITVJ?
NCI-H1155 NUPmOXE1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTBwNkWzOUDPxE1? Mk\3V2FPT0WU
D-263MG M{Lsfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3\HT2lEPTB;MD62OlExOSEQvF2= M{DZ[XNCVkeHUh?=
TE-6 MmDCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTBwNkixNFQh|ryP MUTTRW5ITVJ?
NCI-H1417 NX3scpp7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTBwNkm3NVEh|ryP MWrTRW5ITVJ?
NCI-H510A M4\xdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnf4TWM2OD1yLke3NlM3KM7:TR?= NXq1TJI1W0GQR1XS
KARPAS-45 M3O2NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH3SVHdKSzVyPUCuO|gzOzVizszN M2XYPXNCVkeHUh?=
IST-SL1 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTBwOEW4NlUh|ryP NVrkbFlqW0GQR1XS
COLO-824 NYTvPGJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojETWM2OD1yLki3PFM2KM7:TR?= MlW4V2FPT0WU
NCI-H526 Mle5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\hT3BuUUN3ME2wMlkxQTV6IN88US=> MnXPV2FPT0WU
NCI-H719 MlrXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvnZ4RKSzVyPUCuPVY3PTdizszN MX\TRW5ITVJ?
SF539 NUnHU5JDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LSPGlEPTB;MD65PFM6PiEQvF2= NVnaXVNFW0GQR1XS
NKM-1 NWLQNGNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zhZWlEPTB;MD65PFY3OyEQvF2= NXnERZNyW0GQR1XS
A253 NYjhd2lNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHLOfIJKSzVyPUCuPVg5QDVizszN Ml7RV2FPT0WU
TE-441-T NWG0XW5OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XGcWlEPTB;MT6wOFM2KM7:TR?= M1rYcXNCVkeHUh?=
COR-L88 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLxTWM2OD1zLkGxN|U1KM7:TR?= MVfTRW5ITVJ?
CPC-N MmC1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFu1WJdKSzVyPUGuNVU5QDhizszN NFqwZnZUSU6JRWK=
NCI-H2107 M2Dwd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;4TWM2OD1zLkG3NlYyKM7:TR?= MXjTRW5ITVJ?
ECC4 M4L6TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;LbXFKSzVyPUGuNVg3QTNizszN M324PHNCVkeHUh?=
NCI-H2141 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGSwVmpKSzVyPUGuNVkzQTlizszN MULTRW5ITVJ?
NCI-H1770 NHTiT2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\5SVJIUUN3ME2xMlMzQTJ6IN88US=> NUP1PINPW0GQR1XS
NCI-SNU-1 NFvCV3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDCfIQ2UUN3ME2xMlM{ODRizszN M17aZXNCVkeHUh?=
LU-165 NGXyRnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTFwNEW0Olch|ryP NHe1Om1USU6JRWK=
Raji MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTFwNEeyOVkh|ryP M{nIdnNCVkeHUh?=
EW-24 NWrjZXRJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\oOmlEPTB;MT61OFYyQSEQvF2= M2CyWXNCVkeHUh?=
KP-N-RT-BM-1 MnKzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3ybGdPUUN3ME2xMlU3PTVzIN88US=> MXPTRW5ITVJ?
SK-MEL-1 NUXC[I9sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkTqTWM2OD1zLk[0PFc4KM7:TR?= NILINnJUSU6JRWK=
SCLC-21H NXzWeG9LT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTFwNk[2OVEh|ryP NF\uT|JUSU6JRWK=
SIMA NXXub3ltT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnETWM2OD1zLk[4NlM{KM7:TR?= M4D5[3NCVkeHUh?=
NCI-H1304 M{nY[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGCze5hKSzVyPUGuO|A4PjVizszN M1\zfHNCVkeHUh?=
REH MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTFwN{GwO|gh|ryP NGrvWWFUSU6JRWK=
KP-N-YS MlnVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTFwOUO1OVEh|ryP MkX5V2FPT0WU
DMS-153 MnvyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTFwOUe1PUDPxE1? NHrYfGxUSU6JRWK=
NB10 NEK3SXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfsenNKSzVyPUKuNlA5PzhizszN M1rE[nNCVkeHUh?=
NCI-H345 NHLRbIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUnTZo5nUUN3ME2yMlYyQTB3IN88US=> NITneVFUSU6JRWK=
SK-N-FI MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTJwN{WzPFEh|ryP MknnV2FPT0WU
NCI-H1092 M3PPOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVe3Oo1KUUN3ME2yMlk2Ozl2IN88US=> MYTTRW5ITVJ?
KM-H2 M1fIZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1K2bmlEPTB;Mz62OVM6PiEQvF2= MVvTRW5ITVJ?
KG-1 NYrEc5dOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUKxR|IzUUN3ME2zMlcyPjh6IN88US=> MWDTRW5ITVJ?
D-283MED NFztPIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkPBTWM2OD1|Lke1PFch|ryP M2S3bHNCVkeHUh?=
LU-65 NI\ub|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LtS2lEPTB;ND6xNVQ5PSEQvF2= M{PGNHNCVkeHUh?=
MSTO-211H MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M174O2lEPTB;ND6xPFg6QCEQvF2= NIe3VotUSU6JRWK=
NCI-H2081 NH;HVW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTQTWM2OD12LkK5NVc1KM7:TR?= Moi1V2FPT0WU
D-247MG MnPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTRwNEOxPVEh|ryP NIHtOZBUSU6JRWK=
P31-FUJ NYSxXYJFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3mwd2lEPTB;ND60OVAyQSEQvF2= NWDmU3hVW0GQR1XS
DMS-79 NFmxT3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLITWM2OD12LkmzNVg{KM7:TR?= MW\TRW5ITVJ?
KASUMI-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTRwOUe1NFkh|ryP MWjTRW5ITVJ?
JVM-3 M{DIcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4POOWlEPTB;NT6yOVAyPCEQvF2= MW\TRW5ITVJ?
LAN-6 MnvBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXETYI2UUN3ME21MlUyPzN4IN88US=> NFjjc5BUSU6JRWK=
DJM-1 NIjP[GhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3i2TmlEPTB;NT61OlAzQSEQvF2= MXzTRW5ITVJ?
JVM-2 MoTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojRTWM2OD13Lk[3NFkzKM7:TR?= NISxNYFUSU6JRWK=
U-87-MG NI\6OnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vWOGlEPTB;NT64PVk3PCEQvF2= Mn\NV2FPT0WU
SHP-77 Mn;oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7lTWM2OD13Lkm0PVI5KM7:TR?= Ml\1V2FPT0WU
RPMI-8866 M{\HWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2WwXmlEPTB;Nj6yO|AyOyEQvF2= NVzL[4ZDW0GQR1XS
ES7 NIGwWnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTZwM{m5NlQh|ryP NXjKZ2Q6W0GQR1XS
WSU-NHL NIjTNW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzzTWM2OD14LkWxOlA6KM7:TR?= M2nKcnNCVkeHUh?=
CAS-1 Ml75S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4f4R2lEPTB;Nz6wPVc1QSEQvF2= NX[zZ3FtW0GQR1XS
MHH-NB-11 Mn[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnwTFZKSzVyPUiuNlMyPTJizszN MUTTRW5ITVJ?
ES5 MofoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LqbGlEPTB;OT61NlI5PiEQvF2= NI\rXGhUSU6JRWK=
HDLM-2 M17Cdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfkTWM2OD17Lkm1PVAzKM7:TR?= MmfTV2FPT0WU
NCI-H2171 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTFyLkW5Nlch|ryP MWjTRW5ITVJ?
LNCaP-Clone-FGC M1zmeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Moe4TWM2OD1zMT6zO|E5KM7:TR?= MkHFV2FPT0WU
NCI-H1395 Mm\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTF{LkK2PVkh|ryP MYDTRW5ITVJ?
NCI-H1436 NWTKbFJ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTF|LkOxPVUh|ryP MnTNV2FPT0WU
RCC10RGB MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGOwc3RKSzVyPUGzMlMzOjFizszN NWrVWY0yW0GQR1XS
SW684 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUn2bGxmUUN3ME2xN{46Pzl5IN88US=> NX7HVJd[W0GQR1XS
NCI-H1650 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnnVI5IUUN3ME2xOU4xPDR5IN88US=> M{C1cHNCVkeHUh?=
EW-13 NWrOXFdiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXFR4dlUUN3ME2xOU4zODJzIN88US=> M3PkTXNCVkeHUh?=
EC-GI-10 MmSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHiXmRKSzVyPUG1Mlg3PDRizszN M3r1c3NCVkeHUh?=
CHP-126 MlXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\mZ2lEPTB;MUeuNVkyQCEQvF2= MoPBV2FPT0WU
NCI-H226 NVLOd|BKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnVTJFKSzVyPUG4MlA1ODNizszN MmrKV2FPT0WU
NCI-H1838 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFizcnRKSzVyPUKwMlE5PDhizszN NGeyXmlUSU6JRWK=
EW-11 NH3q[GFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2r2PGlEPTB;MkSuNlcyQCEQvF2= M4PLNXNCVkeHUh?=
EB2 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rOS2lEPTB;M{GuOVMyQCEQvF2= M1zsO3NCVkeHUh?=
D-502MG M1LYUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkOxTWM2OD12Mz65NlI6KM7:TR?= NEKybHlUSU6JRWK=
U-698-M MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTR6Lki2O|Eh|ryP NF;WPY5USU6JRWK=
ES3 M4LoUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXQTWM2OD12OT6zPFk{KM7:TR?= NEj0V2dUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol

Kinase Assay:[1]
- Collapse

mTORC1 and mTORC2 in Vitro Kinase Assays:

To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research:[1]
- Collapse
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: U87MG xenograft model
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage powder
in solvent
Synonyms N/A
Smiles CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F

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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to re-constitute the inhibitor for i.p. administration?

  • Answer:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

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