Torin 1

Catalog No.S2827

Torin 1 Chemical Structure

Molecular Weight(MW): 607.62

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

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Cited by 24 Publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 Ml;rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwMECwN|A3KM7:TR?= MXTTRW5ITVJ?
OCUB-M NU[4eVR4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\lSGNKSzVyPUCuNFAyQTRizszN MXPTRW5ITVJ?
DOHH-2 NYPkOmxjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrITWM2OD1yLkCwNlI2KM7:TR?= MWjTRW5ITVJ?
NOS-1 MlfWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTpTWM2OD1yLkCwNlQzKM7:TR?= NXHVTllbW0GQR1XS
L-363 NIXDcGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTBwMECyPFQh|ryP NHS5bJFUSU6JRWK=
RL95-2 MnvOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTBwMECzNkDPxE1? MoTHV2FPT0WU
BL-70 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjFTWM2OD1yLkCwN|M4KM7:TR?= MWrTRW5ITVJ?
TGBC1TKB MnPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmjOTWM2OD1yLkCwN|c6KM7:TR?= MnmxV2FPT0WU
697 NXLnNHJXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[wS3BKSzVyPUCuNFA3OzRizszN M3LnXXNCVkeHUh?=
RPMI-8226 M4DISGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX7tcJBXUUN3ME2wMlAxPjVzIN88US=> M2LQO3NCVkeHUh?=
BL-41 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTBwMEC2PVYh|ryP MVLTRW5ITVJ?
MRK-nu-1 NVTzO2dPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7kTWM2OD1yLkCwO|A4KM7:TR?= NIfvS|FUSU6JRWK=
EW-1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTBwMEC3OFkh|ryP NEPVboZUSU6JRWK=
EVSA-T M4\4Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vUOWlEPTB;MD6wNFg6OiEQvF2= MnHIV2FPT0WU
HUTU-80 M2TvRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2P6Z2lEPTB;MD6wNFkzQSEQvF2= NIrGdXRUSU6JRWK=
VA-ES-BJ NX\XdZdCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HuOGlEPTB;MD6wNFk5PCEQvF2= M1TvdHNCVkeHUh?=
GCIY M{fNfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvMdW5KSzVyPUCuNFExODdizszN MWrTRW5ITVJ?
ES6 MlHBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LCd2lEPTB;MD6wNVA5OyEQvF2= MlTrV2FPT0WU
NALM-6 NGTwfXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFq2WmxKSzVyPUCuNFEyODJizszN NG\XeFZUSU6JRWK=
LB996-RCC NVnuNIR6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXjZXc{UUN3ME2wMlAyOTN5IN88US=> M3jEZ3NCVkeHUh?=
EW-16 MkKwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LZUmlEPTB;MD6wNVE6OiEQvF2= M4q5cnNCVkeHUh?=
KALS-1 NUX4e|ViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEezTohKSzVyPUCuNFEyQThizszN NXj5SXBQW0GQR1XS
OPM-2 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTvTWM2OD1yLkCxNlkh|ryP NUnFXGpHW0GQR1XS
KU812 M3PleWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIH3RnlKSzVyPUCuNFEzQTZizszN NGTITnRUSU6JRWK=
A4-Fuk M4rsZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTaZlBKSzVyPUCuNFE{OTlizszN M2DUfHNCVkeHUh?=
KGN M3vwfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk[0TWM2OD1yLkCxN|U6KM7:TR?= M1XiSHNCVkeHUh?=
KS-1 MlrvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2POeWlEPTB;MD6wNVM6OiEQvF2= M3XX[3NCVkeHUh?=
no-11 MkHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPYXVV6UUN3ME2wMlAyOzl3IN88US=> MVXTRW5ITVJ?
PSN1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTBwMEG0NFkh|ryP NGfu[ZNUSU6JRWK=
SK-LMS-1 NYThPVJxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTBwMEG0NVMh|ryP MoDXV2FPT0WU
LS-411N M4nMfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTBwMEG0N|kh|ryP NXe4PHdTW0GQR1XS
HT-144 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEflNHBKSzVyPUCuNFE1PTFizszN NGPyZnVUSU6JRWK=
ACN MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGS2So9KSzVyPUCuNFE2KM7:TR?= NUjJ[VJlW0GQR1XS
RKO NXnwdnRlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTBwMEG1N|Mh|ryP M2\IO3NCVkeHUh?=
CMK MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7Md2hmUUN3ME2wMlAyPTR|IN88US=> MoTRV2FPT0WU
GB-1 NIK3flRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\WTWM2OD1yLkCxOVU{KM7:TR?= MULTRW5ITVJ?
KE-37 MlPJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHyXmFKSzVyPUCuNFE2PzNizszN MmDLV2FPT0WU
no-10 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTBwMEG1PFQh|ryP NFjmZmRUSU6JRWK=
MOLT-16 M4W4b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEH1PYNKSzVyPUCuNFE3OjJizszN MlKwV2FPT0WU
HEL MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF73ZYpKSzVyPUCuNFE3PCEQvF2= MVrTRW5ITVJ?
NCI-H1355 NYn4fFBwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLPTWM2OD1yLkCxOlQ3KM7:TR?= NFi0dYpUSU6JRWK=
IST-MEL1 NUXufolRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHGfXJKSzVyPUCuNFE4PzRizszN NYT4Too6W0GQR1XS
MHH-PREB-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTBwMEG4NVgh|ryP MlS5V2FPT0WU
ES8 MlHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHlTWM2OD1yLkCxPFQ1KM7:TR?= MVLTRW5ITVJ?
LC-2-ad NVn4fnNZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFqzSIRKSzVyPUCuNFIyOzdizszN MXjTRW5ITVJ?
LAMA-84 NVnyN3BiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGO3WllKSzVyPUCuNFIyPjdizszN MXvTRW5ITVJ?
BV-173 NWTDbpNKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIC4c49KSzVyPUCuNFIzODNizszN MXvTRW5ITVJ?
SW872 M3Xr[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XmeWlEPTB;MD6wNlI1PyEQvF2= Mn[zV2FPT0WU
SK-UT-1 Mm\GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mof2TWM2OD1yLkCyNlYyKM7:TR?= NIr3R4pUSU6JRWK=
OS-RC-2 M33Vfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzLTWM2OD1yLkCyN|I{KM7:TR?= NHXzS3ZUSU6JRWK=
NCI-H747 NULVSGNOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7VTWM2OD1yLkCyN|Mh|ryP MV\TRW5ITVJ?
MZ2-MEL NVzzNFFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTBwMEKzN|ch|ryP MW\TRW5ITVJ?
TE-11 M3XyPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTBwMEKzO|Ih|ryP NVf3[5JbW0GQR1XS
KURAMOCHI M2npbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;LUmlEPTB;MD6wNlQyPCEQvF2= NHr1NGNUSU6JRWK=
HD-MY-Z M3fyVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEe5d4xKSzVyPUCuNFI1OjZizszN NH3hV|ZUSU6JRWK=
GI-1 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XGU2lEPTB;MD6wNlQ3QSEQvF2= M3LURXNCVkeHUh?=
LB1047-RCC NFfocnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17HN2lEPTB;MD6wNlQ5QCEQvF2= MV\TRW5ITVJ?
TE-12 NID6b2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjZUFJKSzVyPUCuNFI2PSEQvF2= MV\TRW5ITVJ?
MZ7-mel M1zZeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorKTWM2OD1yLkCyOlA6KM7:TR?= NY\YTFJGW0GQR1XS
MMAC-SF NYC4R3J6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITX[nRKSzVyPUCuNFI3OzNizszN M3vhVHNCVkeHUh?=
A101D MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTBwMEK2OUDPxE1? NGTiUXdUSU6JRWK=
GR-ST M4fqT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTBwMEK2OVUh|ryP NX;YO4tvW0GQR1XS
MC116 NIH2RZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXY[HEyUUN3ME2wMlAzPjhizszN NUTHVGtCW0GQR1XS
Ramos-2G6-4C10 MmTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjiZVNsUUN3ME2wMlAzPzVizszN Mke3V2FPT0WU
ATN-1 M3OyOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLxPGk5UUN3ME2wMlAzPzh7IN88US=> MkXSV2FPT0WU
MFH-ino MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7qT|FKSzVyPUCuNFI5OTJizszN NI[1VoRUSU6JRWK=
DEL MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfITY9KSzVyPUCuNFI5OTVizszN M2j1bXNCVkeHUh?=
OCI-AML2 MlXXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrhVGlKSzVyPUCuNFI5PzFizszN NHflTlBUSU6JRWK=
LXF-289 NE\KZ|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXfPR3BsUUN3ME2wMlAzQDd{IN88US=> NWnNUmdZW0GQR1XS
IA-LM NVnzRlN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVj3[|ZTUUN3ME2wMlAzQTF5IN88US=> NXLoZXVMW0GQR1XS
EoL-1-cell NWTIU3ZYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYP0bYdUUUN3ME2wMlAzQTl4IN88US=> NWHaSVZPW0GQR1XS
OVCAR-4 NW\uOoI3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTBwMEOwPVQh|ryP Mle0V2FPT0WU
ES1 M13jcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2K0VmlEPTB;MD6wN|A6PyEQvF2= MV3TRW5ITVJ?
CESS MkXOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTBwMEOwPVkh|ryP Mli5V2FPT0WU
GAK M1f3cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIGxfIpKSzVyPUCuNFMyQDZizszN MYLTRW5ITVJ?
NTERA-S-cl-D1 MnXpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4H1WmlEPTB;MD6wN|IzPiEQvF2= MVHTRW5ITVJ?
TE-10 MljDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rrV2lEPTB;MD6wN|MyOSEQvF2= M3jNeHNCVkeHUh?=
CCRF-CEM MkXZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fKXWlEPTB;MD6wN|M4OSEQvF2= M1:zR3NCVkeHUh?=
NMC-G1 M3;PTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTBwMEO0OFgh|ryP M3nP[3NCVkeHUh?=
HOP-62 Mn;nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTsTWM2OD1yLkCzOVU3KM7:TR?= NGPUblBUSU6JRWK=
SH-4 MoroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LMTGlEPTB;MD6wN|U5OiEQvF2= NFPaUlJUSU6JRWK=
NCI-H1648 NWDmcI94T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\sTWM2OD1yLkCzOlIzKM7:TR?= NELoT2pUSU6JRWK=
EB-3 NEKySWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDBXJRFUUN3ME2wMlA{PjR|IN88US=> NFzkd5pUSU6JRWK=
BB65-RCC MnHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTBwMEO2PVEh|ryP NWDMWJJOW0GQR1XS
BE-13 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTBwMEO3OVkh|ryP NH23bXdUSU6JRWK=
CP67-MEL Mn3KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkCxTWM2OD1yLkCzO|U6KM7:TR?= NH;Tc3hUSU6JRWK=
SW954 NXv5XVB5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfBeYFKSzVyPUCuNFM4QSEQvF2= NYjLZopMW0GQR1XS
TE-5 Mo[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LOW2lEPTB;MD6wN|g2PCEQvF2= Ml;4V2FPT0WU
HCE-4 NITiR|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzNN4tKSzVyPUCuNFM5PTVizszN NV6wZ|BXW0GQR1XS
LC-1F M4izRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rzTmlEPTB;MD6wN|k5QSEQvF2= NWj6VGhwW0GQR1XS
TK10 NFvlSY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2OydGlEPTB;MD6wOFAyQSEQvF2= M2npOnNCVkeHUh?=
SJSA-1 NFXYSG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTBwMESxPFgh|ryP NVrJ[4NFW0GQR1XS
Daudi NHnlPWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvRXpI2UUN3ME2wMlA1OjJ3IN88US=> NH7PT5NUSU6JRWK=
SW982 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3T0ZmlEPTB;MD6wOFI1PiEQvF2= MlHpV2FPT0WU
SF126 M2nndmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHi5c2JKSzVyPUCuNFQ{PjFizszN MXTTRW5ITVJ?
TE-15 M{SxfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1OzemlEPTB;MD6wOFQ{PSEQvF2= MV;TRW5ITVJ?
COLO-684 M1i1Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HFXGlEPTB;MD6wOFU3OSEQvF2= MnewV2FPT0WU
8-MG-BA NWrzfodOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnsUHZYUUN3ME2wMlA1PjJ7IN88US=> MYTTRW5ITVJ?
NB13 M3\JRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTBwMES3O|Yh|ryP NU\IWFJNW0GQR1XS
HCC1599 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmSwTWM2OD1yLkC0PFc6KM7:TR?= NFfZUYVUSU6JRWK=
SK-PN-DW NE[1R3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nWb2lEPTB;MD6wOFk1QSEQvF2= NFzidnNUSU6JRWK=
ALL-PO MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfDOFY2UUN3ME2wMlA1QTV4IN88US=> MUjTRW5ITVJ?
NB6 NWG4eGFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTBwMEWyO|Eh|ryP MlWyV2FPT0WU
IST-SL2 NILweJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXRTWM2OD1yLkC1N|YzKM7:TR?= NH7HdIpUSU6JRWK=
BB30-HNC NV25WXB1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPJc4xpUUN3ME2wMlA2PzNizszN MUjTRW5ITVJ?
SW962 MlTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MorpTWM2OD1yLkC1O|Y{KM7:TR?= MYrTRW5ITVJ?
TGBC24TKB M2PtN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX[4dYR2UUN3ME2wMlA2Pzl7IN88US=> NHLKdZZUSU6JRWK=
KINGS-1 MoHPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDpTWM2OD1yLkC1PFA{KM7:TR?= Mn6xV2FPT0WU
HC-1 MkflS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjyOYhKSzVyPUCuNFU5OjdizszN NFPqeoRUSU6JRWK=
RS4-11 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPzVowzUUN3ME2wMlA2QTN6IN88US=> MYDTRW5ITVJ?
LS-123 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLaSmtzUUN3ME2wMlA2QTR{IN88US=> NUXaeYZHW0GQR1XS
A3-KAW M{K4PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV36VJFPUUN3ME2wMlA3OjJ3IN88US=> M3zWSXNCVkeHUh?=
SNB75 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnCclZKSzVyPUCuNFY{PDFizszN NUDMOXpoW0GQR1XS
SR NVTuUmd2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIC1epVKSzVyPUCuNFY{PzRizszN NW[4OFZ6W0GQR1XS
LOUCY NGm4XYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwME[zPVQh|ryP MnHMV2FPT0WU
K5 Mk\6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn[0TWM2OD1yLkC2OFA{KM7:TR?= NGnnNZBUSU6JRWK=
Becker MlPBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XhU2lEPTB;MD6wOlQzKM7:TR?= NEHESHRUSU6JRWK=
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HCC1187 NHnP[lFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4mwe2lEPTB;MD60Olc3QCEQvF2= M2jjNnNCVkeHUh?=
HCC2218 NEW5clBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTBwNEeyNlch|ryP M2T5d3NCVkeHUh?=
NCI-H187 NELPOoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTBwNEe4OEDPxE1? MlTxV2FPT0WU
DB Mnr1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTBwNUCwOVUh|ryP NEXwOo5USU6JRWK=
NCI-H69 NGTmOYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nMeWlEPTB;MD61NFI1PyEQvF2= M1LaOHNCVkeHUh?=
NCI-H1694 MmKwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTBwNUC2PVch|ryP NW\XUnFNW0GQR1XS
CA46 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7P[ZJKSzVyPUCuOVA6OTdizszN M4rIdHNCVkeHUh?=
EHEB MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTBwNUGxPVMh|ryP M{nCfHNCVkeHUh?=
NCCIT MlTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7uTWM2OD1yLkW0N|IyKM7:TR?= NGHJVotUSU6JRWK=
NCI-H720 NH7t[GJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTBwNUS5OlIh|ryP NFfKUGhUSU6JRWK=
LU-134-A NYS2WIdVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnkTWM2OD1yLkW1Nlk3KM7:TR?= MVLTRW5ITVJ?
MC-CAR NHP4PXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzFc2dVUUN3ME2wMlU2PDNzIN88US=> NYq5c5N7W0GQR1XS
NEC8 NGfXbVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH20b5hKSzVyPUCuOVYyPzJizszN NXzXdpZiW0GQR1XS
NCI-H524 M3ewfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrRTWM2OD1yLkW4O|Q5KM7:TR?= MVnTRW5ITVJ?
NCI-H1963 NWLNfVhET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7vRpdKSzVyPUCuOlI2QCEQvF2= NHrLdGZUSU6JRWK=
SU-DHL-1 M3PScmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjXVIRLUUN3ME2wMlY1PTJ4IN88US=> NVXxV2NmW0GQR1XS
NCI-H1522 NUmxblE3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LpPWlEPTB;MD62OFg5OyEQvF2= NH:3TY1USU6JRWK=
NCI-H1155 M{jqZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHFRoV6UUN3ME2wMlY2OzVizszN M2rIR3NCVkeHUh?=
D-263MG NI\RZZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmj0TWM2OD1yLk[2NVAyKM7:TR?= MWrTRW5ITVJ?
TE-6 NH7ySphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TIfWlEPTB;MD62PFExPCEQvF2= MljBV2FPT0WU
NCI-H1417 M3vQdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXq4fFJ3UUN3ME2wMlY6PzFzIN88US=> M{\nTXNCVkeHUh?=
NCI-H510A NHmw[pVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjkO|dKSzVyPUCuO|czOzZizszN MlvTV2FPT0WU
KARPAS-45 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUm5UJpKUUN3ME2wMlc5OjN3IN88US=> NGX2SWtUSU6JRWK=
IST-SL1 NVThTpVoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmr1TWM2OD1yLki1PFI2KM7:TR?= NEPXeWtUSU6JRWK=
COLO-824 M2\HSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTBwOEe4N|Uh|ryP MWjTRW5ITVJ?
NCI-H526 NX\POGpUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn[wTWM2OD1yLkmwPVU5KM7:TR?= M2nTbHNCVkeHUh?=
NCI-H719 M3fsXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUGyNI1KUUN3ME2wMlk3PjV5IN88US=> NEfvUXZUSU6JRWK=
SF539 MkDGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;mTWM2OD1yLkm4N|k3KM7:TR?= NEHoXW5USU6JRWK=
NKM-1 MoHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HRb2lEPTB;MD65PFY3OyEQvF2= Mn7YV2FPT0WU
A253 M3XSfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXS4RVRXUUN3ME2wMlk5QDh3IN88US=> NHLwRnhUSU6JRWK=
TE-441-T NYPiepNmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlzGTWM2OD1zLkC0N|Uh|ryP MXzTRW5ITVJ?
COR-L88 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfvW5lKSzVyPUGuNVE{PTRizszN NXLJWWJYW0GQR1XS
CPC-N M2jZXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTFwMUW4PFgh|ryP NEXPepJUSU6JRWK=
NCI-H2107 NEj3Wm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTFwMUeyOlEh|ryP MXXTRW5ITVJ?
ECC4 Mkn4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIOwPXFKSzVyPUGuNVg3QTNizszN MlTDV2FPT0WU
NCI-H2141 NHPmNnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTFwMUmyPVkh|ryP MnvKV2FPT0WU
NCI-H1770 M2[4RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnpTWM2OD1zLkOyPVI5KM7:TR?= MVvTRW5ITVJ?
NCI-SNU-1 NELIXXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\TTWM2OD1zLkOzNFQh|ryP MkPPV2FPT0WU
LU-165 MmL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXUdZdIUUN3ME2xMlQ2PDZ5IN88US=> MlvLV2FPT0WU
Raji NHH6dXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PGZWlEPTB;MT60O|I2QSEQvF2= MlnVV2FPT0WU
EW-24 M4LRe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LUW2lEPTB;MT61OFYyQSEQvF2= MonYV2FPT0WU
KP-N-RT-BM-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTFwNU[1OVEh|ryP M2TVXnNCVkeHUh?=
SK-MEL-1 NWDCeWpjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjsc2hKSzVyPUGuOlQ5PzdizszN NYezPHZMW0GQR1XS
SCLC-21H NWnHcVlNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTFwNk[2OVEh|ryP NHv3foVUSU6JRWK=
SIMA MnLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\IbZVKUUN3ME2xMlY5OjN|IN88US=> MVHTRW5ITVJ?
NCI-H1304 MnHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{eyZWlEPTB;MT63NFc3PSEQvF2= NUTBdZB{W0GQR1XS
REH MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3L3RWlEPTB;MT63NVA4QCEQvF2= NXzMZ4h6W0GQR1XS
KP-N-YS MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;vSYtRUUN3ME2xMlk{PTVzIN88US=> Mmi1V2FPT0WU
DMS-153 NI\zc4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTFwOUe1PUDPxE1? M3\LR3NCVkeHUh?=
NB10 NXTpfJBLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn71TWM2OD1{LkKwPFc5KM7:TR?= MnrpV2FPT0WU
NCI-H345 MkTNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2TndGlEPTB;Mj62NVkxPSEQvF2= MUHTRW5ITVJ?
SK-N-FI M2LvVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXe4N4FwUUN3ME2yMlc2OzhzIN88US=> MmPoV2FPT0WU
NCI-H1092 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFu4NXlKSzVyPUKuPVU{QTRizszN Mli2V2FPT0WU
KM-H2 MkTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFL6U2dKSzVyPUOuOlU{QTZizszN NEnMUIFUSU6JRWK=
KG-1 NVL4fpJiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHKdZlKSzVyPUOuO|E3QDhizszN MlzEV2FPT0WU
D-283MED MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPuPJdKSzVyPUOuO|U5PyEQvF2= MY\TRW5ITVJ?
LU-65 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7hPWVrUUN3ME20MlEyPDh3IN88US=> MV7TRW5ITVJ?
MSTO-211H NHW4fmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\CTWM2OD12LkG4PFk5KM7:TR?= NHO4e5VUSU6JRWK=
NCI-H2081 M37zZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXviTYZwUUN3ME20MlI6OTd2IN88US=> NIHPNW9USU6JRWK=
D-247MG NIXh[ZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIO4VodKSzVyPUSuOFMyQTFizszN MlfXV2FPT0WU
P31-FUJ MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\BXGlEPTB;ND60OVAyQSEQvF2= Mon6V2FPT0WU
DMS-79 NWS1b2xST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nVd2lEPTB;ND65N|E5OyEQvF2= NVTXOlhTW0GQR1XS
KASUMI-1 NILVcGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TxZmlEPTB;ND65O|UxQSEQvF2= MkHpV2FPT0WU
JVM-3 NYD3[ZNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDSeG9KSzVyPUWuNlUxOTRizszN MkTsV2FPT0WU
LAN-6 MlH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jGO2lEPTB;NT61NVc{PiEQvF2= NF7nR3pUSU6JRWK=
DJM-1 NWLuOmZsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;5U5FKSzVyPUWuOVYxOjlizszN M1\YSHNCVkeHUh?=
JVM-2 MnXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFz1eZFKSzVyPUWuOlcxQTJizszN NVTBV|J4W0GQR1XS
U-87-MG NVnKV|VHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\5Z3JyUUN3ME21Mlg6QTZ2IN88US=> MVHTRW5ITVJ?
SHP-77 M1T2O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjr[VJDUUN3ME21Mlk1QTJ6IN88US=> NEDU[lFUSU6JRWK=
RPMI-8866 MmryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTZwMkewNVMh|ryP MWHTRW5ITVJ?
ES7 M4T5O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\SdnhTUUN3ME22MlM6QTJ2IN88US=> NVPBTZZ7W0GQR1XS
WSU-NHL NG\3fIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDPTphHUUN3ME22MlUyPjB7IN88US=> MWHTRW5ITVJ?
CAS-1 M1PDVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTdwMEm3OFkh|ryP NXXmdYd2W0GQR1XS
MHH-NB-11 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfaTWM2OD16LkKzNVUzKM7:TR?= NYr4bIxJW0GQR1XS
ES5 MnG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIizfpZKSzVyPUmuOVIzQDZizszN NWHrUXBoW0GQR1XS
HDLM-2 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTlwOUW5NFIh|ryP MmLxV2FPT0WU
NCI-H2171 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTFyLkW5Nlch|ryP MXPTRW5ITVJ?
LNCaP-Clone-FGC NHTLVo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3K1VWlEPTB;MUGuN|cyQCEQvF2= MXLTRW5ITVJ?
NCI-H1395 NH\QRVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTF{LkK2PVkh|ryP MoXtV2FPT0WU
NCI-H1436 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LhUWlEPTB;MUOuN|E6PSEQvF2= M1jRWHNCVkeHUh?=
RCC10RGB MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PPfmlEPTB;MUOuN|IzOSEQvF2= NEHkNo1USU6JRWK=
SW684 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHP4cFdKSzVyPUGzMlk4QTdizszN MVrTRW5ITVJ?
NCI-H1650 M{T1bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVv4dpFXUUN3ME2xOU4xPDR5IN88US=> M2PLb3NCVkeHUh?=
EW-13 MnvwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\PfWlEPTB;MUWuNlAzOSEQvF2= MYPTRW5ITVJ?
EC-GI-10 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXPeW0{UUN3ME2xOU45PjR2IN88US=> M4PwXXNCVkeHUh?=
CHP-126 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkPnTWM2OD1zNz6xPVE5KM7:TR?= M3zvOXNCVkeHUh?=
NCI-H226 M4\2[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17oR2lEPTB;MUiuNFQxOyEQvF2= M1zRXHNCVkeHUh?=
NCI-H1838 NXTYOI4zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLZbnY2UUN3ME2yNE4yQDR6IN88US=> Ml;CV2FPT0WU
EW-11 NFewOo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3RPIRKSzVyPUK0MlI4OThizszN M{W0OnNCVkeHUh?=
EB2 M1;FRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\LTWM2OD1|MT61N|E5KM7:TR?= MX\TRW5ITVJ?
D-502MG M3\rPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\WTWM2OD12Mz65NlI6KM7:TR?= Mk\WV2FPT0WU
U-698-M NYLwZYxnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUK3eos2UUN3ME20PE45PjdzIN88US=> MoT0V2FPT0WU
ES3 M4\obWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LJWmlEPTB;NEmuN|g6OyEQvF2= M1zPUXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol

Kinase Assay:[1]
+ Expand

mTORC1 and mTORC2 in Vitro Kinase Assays:

To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research:[1]
+ Expand
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
    (Only for Reference)
Animal Research:[2]
+ Expand
  • Animal Models: U87MG xenograft model
  • Formulation: Torin1 powder is first dissolved at 25 mg/mL in 100% N-methyl-2-pyrrolidone and subsequently diluted 1:4 with sterile 50% PEG400 to a final concentration of 5 mg/mL
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
20% NMP+50% PEG 400
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to re-constitute the inhibitor for i.p. administration?

  • Answer:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID