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- Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
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Torin 1
Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Torin 1 Chemical Structure
CAS No. 1222998-36-8
Purity & Quality Control
Batch:
Purity:
99.07%
99.07
Products often used together with Torin 1
Torin 1 and Rapamycin differentially regulate the iron-induced generation of mitochondrial ROS in human neuroblastoma cells.
Torin 1 Related Products
| Related Targets | mTORC1 mTORC2 | Click to Expand |
|---|---|---|
| Related Products | AZD8055 Ridaforolimus (Deforolimus, MK-8669) Sapanisertib (MLN0128) Torkinib (PP242) Vistusertib (AZD2014) MHY1485 KU-0063794 Torin 2 OSI-027 WYE-354 WYE-125132 (WYE-132) 3BDO Palomid 529 (P529) Zotarolimus (ABT-578) Salidroside GDC-0349 WAY-600 Onatasertib (CC 223) XL388 WYE-687 | Click to Expand |
| Related Compound Libraries | Kinase Inhibitor Library PI3K/Akt Inhibitor Library Apoptosis Compound Library Cell Cycle compound library NF-κB Signaling Compound Library | Click to Expand |
Signaling Pathway
Cell Data
| Cell Lines | Assay Type | Concentration | Incubation Time | Formulation | Activity Description | PMID |
|---|---|---|---|---|---|---|
| ETK-1 | Growth Inhibition Assay | IC50=0.000306 μM | SANGER | |||
| OCUB-M | Growth Inhibition Assay | IC50=0.00194 μM | SANGER | |||
| DOHH-2 | Growth Inhibition Assay | IC50=0.00225 μM | SANGER | |||
| NOS-1 | Growth Inhibition Assay | IC50=0.00242 μM | SANGER | |||
| L-363 | Growth Inhibition Assay | IC50=0.00284 μM | SANGER | |||
| RL95-2 | Growth Inhibition Assay | IC50=0.0032 μM | SANGER | |||
| BL-70 | Growth Inhibition Assay | IC50=0.00337 μM | SANGER | |||
| TGBC1TKB | Growth Inhibition Assay | IC50=0.00379 μM | SANGER | |||
| 697 | Growth Inhibition Assay | IC50=0.00634 μM | SANGER | |||
| RPMI-8226 | Growth Inhibition Assay | IC50=0.00651 μM | SANGER | |||
| BL-41 | Growth Inhibition Assay | IC50=0.00696 μM | SANGER | |||
| MRK-nu-1 | Growth Inhibition Assay | IC50=0.00707 μM | SANGER | |||
| EW-1 | Growth Inhibition Assay | IC50=0.00749 μM | SANGER | |||
| EVSA-T | Growth Inhibition Assay | IC50=0.00892 μM | SANGER | |||
| HUTU-80 | Growth Inhibition Assay | IC50=0.00929 μM | SANGER | |||
| VA-ES-BJ | Growth Inhibition Assay | IC50=0.00984 μM | SANGER | |||
| GCIY | Growth Inhibition Assay | IC50=0.01007 μM | SANGER | |||
| ES6 | Growth Inhibition Assay | IC50=0.01083 μM | SANGER | |||
| NALM-6 | Growth Inhibition Assay | IC50=0.01102 μM | SANGER | |||
| LB996-RCC | Growth Inhibition Assay | IC50=0.01137 μM | SANGER | |||
| EW-16 | Growth Inhibition Assay | IC50=0.01192 μM | SANGER | |||
| KALS-1 | Growth Inhibition Assay | IC50=0.01198 μM | SANGER | |||
| OPM-2 | Growth Inhibition Assay | IC50=0.0129 μM | SANGER | |||
| KU812 | Growth Inhibition Assay | IC50=0.01296 μM | SANGER | |||
| A4-Fuk | Growth Inhibition Assay | IC50=0.01319 μM | SANGER | |||
| KGN | Growth Inhibition Assay | IC50=0.01359 μM | SANGER | |||
| KS-1 | Growth Inhibition Assay | IC50=0.01392 μM | SANGER | |||
| no-11 | Growth Inhibition Assay | IC50=0.01395 μM | SANGER | |||
| PSN1 | Growth Inhibition Assay | IC50=0.01409 μM | SANGER | |||
| SK-LMS-1 | Growth Inhibition Assay | IC50=0.01413 μM | SANGER | |||
| LS-411N | Growth Inhibition Assay | IC50=0.01439 μM | SANGER | |||
| HT-144 | Growth Inhibition Assay | IC50=0.01451 μM | SANGER | |||
| ACN | Growth Inhibition Assay | IC50=0.015 μM | SANGER | |||
| RKO | Growth Inhibition Assay | IC50=0.01533 μM | SANGER | |||
| CMK | Growth Inhibition Assay | IC50=0.01543 μM | SANGER | |||
| GB-1 | Growth Inhibition Assay | IC50=0.01553 μM | SANGER | |||
| KE-37 | Growth Inhibition Assay | IC50=0.01573 μM | SANGER | |||
| no-10 | Growth Inhibition Assay | IC50=0.01584 μM | SANGER | |||
| MOLT-16 | Growth Inhibition Assay | IC50=0.01622 μM | SANGER | |||
| HEL | Growth Inhibition Assay | IC50=0.0164 μM | SANGER | |||
| NCI-H1355 | Growth Inhibition Assay | IC50=0.01646 μM | SANGER | |||
| IST-MEL1 | Growth Inhibition Assay | IC50=0.01774 μM | SANGER | |||
| MHH-PREB-1 | Growth Inhibition Assay | IC50=0.01818 μM | SANGER | |||
| ES8 | Growth Inhibition Assay | IC50=0.01844 μM | SANGER | |||
| LC-2-ad | Growth Inhibition Assay | IC50=0.02137 μM | SANGER | |||
| LAMA-84 | Growth Inhibition Assay | IC50=0.02167 μM | SANGER | |||
| BV-173 | Growth Inhibition Assay | IC50=0.02203 μM | SANGER | |||
| SW872 | Growth Inhibition Assay | IC50=0.02247 μM | SANGER | |||
| SK-UT-1 | Growth Inhibition Assay | IC50=0.02261 μM | SANGER | |||
| OS-RC-2 | Growth Inhibition Assay | IC50=0.02323 μM | SANGER | |||
| NCI-H747 | Growth Inhibition Assay | IC50=0.0233 μM | SANGER | |||
| MZ2-MEL | Growth Inhibition Assay | IC50=0.02337 μM | SANGER | |||
| TE-11 | Growth Inhibition Assay | IC50=0.02372 μM | SANGER | |||
| KURAMOCHI | Growth Inhibition Assay | IC50=0.02414 μM | SANGER | |||
| HD-MY-Z | Growth Inhibition Assay | IC50=0.02426 μM | SANGER | |||
| GI-1 | Growth Inhibition Assay | IC50=0.02469 μM | SANGER | |||
| LB1047-RCC | Growth Inhibition Assay | IC50=0.02488 μM | SANGER | |||
| TE-12 | Growth Inhibition Assay | IC50=0.0255 μM | SANGER | |||
| MZ7-mel | Growth Inhibition Assay | IC50=0.02609 μM | SANGER | |||
| MMAC-SF | Growth Inhibition Assay | IC50=0.02633 μM | SANGER | |||
| A101D | Growth Inhibition Assay | IC50=0.0265 μM | SANGER | |||
| GR-ST | Growth Inhibition Assay | IC50=0.02655 μM | SANGER | |||
| MC116 | Growth Inhibition Assay | IC50=0.0268 μM | SANGER | |||
| Ramos-2G6-4C10 | Growth Inhibition Assay | IC50=0.0275 μM | SANGER | |||
| ATN-1 | Growth Inhibition Assay | IC50=0.02789 μM | SANGER | |||
| MFH-ino | Growth Inhibition Assay | IC50=0.02812 μM | SANGER | |||
| DEL | Growth Inhibition Assay | IC50=0.02815 μM | SANGER | |||
| OCI-AML2 | Growth Inhibition Assay | IC50=0.02871 μM | SANGER | |||
| LXF-289 | Growth Inhibition Assay | IC50=0.02872 μM | SANGER | |||
| IA-LM | Growth Inhibition Assay | IC50=0.02917 μM | SANGER | |||
| EoL-1-cell | Growth Inhibition Assay | IC50=0.02996 μM | SANGER | |||
| OVCAR-4 | Growth Inhibition Assay | IC50=0.03094 μM | SANGER | |||
| ES1 | Growth Inhibition Assay | IC50=0.03097 μM | SANGER | |||
| CESS | Growth Inhibition Assay | IC50=0.03099 μM | SANGER | |||
| GAK | Growth Inhibition Assay | IC50=0.03186 μM | SANGER | |||
| NTERA-S-cl-D1 | Growth Inhibition Assay | IC50=0.03226 μM | SANGER | |||
| TE-10 | Growth Inhibition Assay | IC50=0.03311 μM | SANGER | |||
| CCRF-CEM | Growth Inhibition Assay | IC50=0.03371 μM | SANGER | |||
| NMC-G1 | Growth Inhibition Assay | IC50=0.03448 μM | SANGER | |||
| HOP-62 | Growth Inhibition Assay | IC50=0.03556 μM | SANGER | |||
| SH-4 | Growth Inhibition Assay | IC50=0.03582 μM | SANGER | |||
| NCI-H1648 | Growth Inhibition Assay | IC50=0.03622 μM | SANGER | |||
| EB-3 | Growth Inhibition Assay | IC50=0.03643 μM | SANGER | |||
| BB65-RCC | Growth Inhibition Assay | IC50=0.03691 μM | SANGER | |||
| BE-13 | Growth Inhibition Assay | IC50=0.03759 μM | SANGER | |||
| CP67-MEL | Growth Inhibition Assay | IC50=0.03759 μM | SANGER | |||
| SW954 | Growth Inhibition Assay | IC50=0.0379 μM | SANGER | |||
| TE-5 | Growth Inhibition Assay | IC50=0.03854 μM | SANGER | |||
| HCE-4 | Growth Inhibition Assay | IC50=0.03855 μM | SANGER | |||
| LC-1F | Growth Inhibition Assay | IC50=0.03989 μM | SANGER | |||
| TK10 | Growth Inhibition Assay | IC50=0.04019 μM | SANGER | |||
| SJSA-1 | Growth Inhibition Assay | IC50=0.04188 μM | SANGER | |||
| Daudi | Growth Inhibition Assay | IC50=0.04225 μM | SANGER | |||
| SW982 | Growth Inhibition Assay | IC50=0.04246 μM | SANGER | |||
| SF126 | Growth Inhibition Assay | IC50=0.04361 μM | SANGER | |||
| TE-15 | Growth Inhibition Assay | IC50=0.04435 μM | SANGER | |||
| COLO-684 | Growth Inhibition Assay | IC50=0.04561 μM | SANGER | |||
| 8-MG-BA | Growth Inhibition Assay | IC50=0.04629 μM | SANGER | |||
| NB13 | Growth Inhibition Assay | IC50=0.04776 μM | SANGER | |||
| HCC1599 | Growth Inhibition Assay | IC50=0.04879 μM | SANGER | |||
| SK-PN-DW | Growth Inhibition Assay | IC50=0.04949 μM | SANGER | |||
| ALL-PO | Growth Inhibition Assay | IC50=0.04956 μM | SANGER | |||
| NB6 | Growth Inhibition Assay | IC50=0.05271 μM | SANGER | |||
| IST-SL2 | Growth Inhibition Assay | IC50=0.05362 μM | SANGER | |||
| BB30-HNC | Growth Inhibition Assay | IC50=0.0573 μM | SANGER | |||
| SW962 | Growth Inhibition Assay | IC50=0.05763 μM | SANGER | |||
| TGBC24TKB | Growth Inhibition Assay | IC50=0.05799 μM | SANGER | |||
| KINGS-1 | Growth Inhibition Assay | IC50=0.05803 μM | SANGER | |||
| HC-1 | Growth Inhibition Assay | IC50=0.05827 μM | SANGER | |||
| RS4-11 | Growth Inhibition Assay | IC50=0.05938 μM | SANGER | |||
| LS-123 | Growth Inhibition Assay | IC50=0.05942 μM | SANGER | |||
| A3-KAW | Growth Inhibition Assay | IC50=0.06225 μM | SANGER | |||
| SNB75 | Growth Inhibition Assay | IC50=0.06341 μM | SANGER | |||
| SR | Growth Inhibition Assay | IC50=0.06374 μM | SANGER | |||
| LOUCY | Growth Inhibition Assay | IC50=0.06394 μM | SANGER | |||
| K5 | Growth Inhibition Assay | IC50=0.06403 μM | SANGER | |||
| Becker | Growth Inhibition Assay | IC50=0.0642 μM | SANGER | |||
| CTV-1 | Growth Inhibition Assay | IC50=0.06548 μM | SANGER | |||
| KLE | Growth Inhibition Assay | IC50=0.06584 μM | SANGER | |||
| MZ1-PC | Growth Inhibition Assay | IC50=0.06598 μM | SANGER | |||
| MOLT-4 | Growth Inhibition Assay | IC50=0.06605 μM | SANGER | |||
| MLMA | Growth Inhibition Assay | IC50=0.0678 μM | SANGER | |||
| A388 | Growth Inhibition Assay | IC50=0.06893 μM | SANGER | |||
| MV-4-11 | Growth Inhibition Assay | IC50=0.06995 μM | SANGER | |||
| DSH1 | Growth Inhibition Assay | IC50=0.07173 μM | SANGER | |||
| MONO-MAC-6 | Growth Inhibition Assay | IC50=0.07212 μM | SANGER | |||
| ARH-77 | Growth Inhibition Assay | IC50=0.07278 μM | SANGER | |||
| LB771-HNC | Growth Inhibition Assay | IC50=0.07549 μM | SANGER | |||
| PF-382 | Growth Inhibition Assay | IC50=0.07569 μM | SANGER | |||
| KM12 | Growth Inhibition Assay | IC50=0.0764 μM | SANGER | |||
| HAL-01 | Growth Inhibition Assay | IC50=0.07658 μM | SANGER | |||
| RPMI-8402 | Growth Inhibition Assay | IC50=0.07789 μM | SANGER | |||
| OMC-1 | Growth Inhibition Assay | IC50=0.07804 μM | SANGER | |||
| HT | Growth Inhibition Assay | IC50=0.08 μM | SANGER | |||
| QIMR-WIL | Growth Inhibition Assay | IC50=0.08055 μM | SANGER | |||
| KNS-42 | Growth Inhibition Assay | IC50=0.08203 μM | SANGER | |||
| BC-3 | Growth Inhibition Assay | IC50=0.08204 μM | SANGER | |||
| ES4 | Growth Inhibition Assay | IC50=0.08296 μM | SANGER | |||
| NCI-H23 | Growth Inhibition Assay | IC50=0.0844 μM | SANGER | |||
| LB2518-MEL | Growth Inhibition Assay | IC50=0.08696 μM | SANGER | |||
| LC4-1 | Growth Inhibition Assay | IC50=0.08738 μM | SANGER | |||
| SIG-M5 | Growth Inhibition Assay | IC50=0.08904 μM | SANGER | |||
| LP-1 | Growth Inhibition Assay | IC50=0.08938 μM | SANGER | |||
| TE-8 | Growth Inhibition Assay | IC50=0.09016 μM | SANGER | |||
| K-562 | Growth Inhibition Assay | IC50=0.0912 μM | SANGER | |||
| J-RT3-T3-5 | Growth Inhibition Assay | IC50=0.09153 μM | SANGER | |||
| DU-4475 | Growth Inhibition Assay | IC50=0.09165 μM | SANGER | |||
| RXF393 | Growth Inhibition Assay | IC50=0.09462 μM | SANGER | |||
| NB5 | Growth Inhibition Assay | IC50=0.09625 μM | SANGER | |||
| A704 | Growth Inhibition Assay | IC50=0.09741 μM | SANGER | |||
| SF268 | Growth Inhibition Assay | IC50=0.09825 μM | SANGER | |||
| UACC-257 | Growth Inhibition Assay | IC50=0.09833 μM | SANGER | |||
| L-428 | Growth Inhibition Assay | IC50=0.1032 μM | SANGER | |||
| ST486 | Growth Inhibition Assay | IC50=0.10606 μM | SANGER | |||
| SK-MM-2 | Growth Inhibition Assay | IC50=0.10709 μM | SANGER | |||
| COR-L279 | Growth Inhibition Assay | IC50=0.10832 μM | SANGER | |||
| JiyoyeP-2003 | Growth Inhibition Assay | IC50=0.10843 μM | SANGER | |||
| GT3TKB | Growth Inhibition Assay | IC50=0.10918 μM | SANGER | |||
| HCE-T | Growth Inhibition Assay | IC50=0.1094 μM | SANGER | |||
| SBC-1 | Growth Inhibition Assay | IC50=0.11119 μM | SANGER | |||
| KNS-81-FD | Growth Inhibition Assay | IC50=0.11273 μM | SANGER | |||
| NCI-H2126 | Growth Inhibition Assay | IC50=0.11394 μM | SANGER | |||
| EM-2 | Growth Inhibition Assay | IC50=0.11529 μM | SANGER | |||
| JAR | Growth Inhibition Assay | IC50=0.11598 μM | SANGER | |||
| NB17 | Growth Inhibition Assay | IC50=0.11652 μM | SANGER | |||
| LB647-SCLC | Growth Inhibition Assay | IC50=0.11705 μM | SANGER | |||
| CAL-148 | Growth Inhibition Assay | IC50=0.118 μM | SANGER | |||
| K052 | Growth Inhibition Assay | IC50=0.11851 μM | SANGER | |||
| KARPAS-299 | Growth Inhibition Assay | IC50=0.12233 μM | SANGER | |||
| KARPAS-422 | Growth Inhibition Assay | IC50=0.12432 μM | SANGER | |||
| LOXIMVI | Growth Inhibition Assay | IC50=0.12515 μM | SANGER | |||
| SCC-3 | Growth Inhibition Assay | IC50=0.12851 μM | SANGER | |||
| LU-139 | Growth Inhibition Assay | IC50=0.12975 μM | SANGER | |||
| COLO-320-HSR | Growth Inhibition Assay | IC50=0.13086 μM | SANGER | |||
| ONS-76 | Growth Inhibition Assay | IC50=0.1313 μM | SANGER | |||
| U-266 | Growth Inhibition Assay | IC50=0.13245 μM | SANGER | |||
| HH | Growth Inhibition Assay | IC50=0.13535 μM | SANGER | |||
| BB49-HNC | Growth Inhibition Assay | IC50=0.13587 μM | SANGER | |||
| NCI-SNU-5 | Growth Inhibition Assay | IC50=0.13596 μM | SANGER | |||
| C2BBe1 | Growth Inhibition Assay | IC50=0.13746 μM | SANGER | |||
| ML-2 | Growth Inhibition Assay | IC50=0.13953 μM | SANGER | |||
| SCH | Growth Inhibition Assay | IC50=0.13974 μM | SANGER | |||
| NCI-H82 | Growth Inhibition Assay | IC50=0.14104 μM | SANGER | |||
| MDA-MB-134-VI | Growth Inhibition Assay | IC50=0.14252 μM | SANGER | |||
| SK-N-DZ | Growth Inhibition Assay | IC50=0.14719 μM | SANGER | |||
| HL-60 | Growth Inhibition Assay | IC50=0.1492 μM | SANGER | |||
| LB373-MEL-D | Growth Inhibition Assay | IC50=0.15539 μM | SANGER | |||
| LB831-BLC | Growth Inhibition Assay | IC50=0.16076 μM | SANGER | |||
| SK-NEP-1 | Growth Inhibition Assay | IC50=0.16101 μM | SANGER | |||
| IMR-5 | Growth Inhibition Assay | IC50=0.16483 μM | SANGER | |||
| Calu-6 | Growth Inhibition Assay | IC50=0.16537 μM | SANGER | |||
| NCI-H1882 | Growth Inhibition Assay | IC50=0.16728 μM | SANGER | |||
| NCI-H446 | Growth Inhibition Assay | IC50=0.16891 μM | SANGER | |||
| GI-ME-N | Growth Inhibition Assay | IC50=0.1802 μM | SANGER | |||
| MPP-89 | Growth Inhibition Assay | IC50=0.18121 μM | SANGER | |||
| CGTH-W-1 | Growth Inhibition Assay | IC50=0.18436 μM | SANGER | |||
| MEG-01 | Growth Inhibition Assay | IC50=0.18628 μM | SANGER | |||
| NCI-H748 | Growth Inhibition Assay | IC50=0.18911 μM | SANGER | |||
| COLO-829 | Growth Inhibition Assay | IC50=0.20673 μM | SANGER | |||
| GOTO | Growth Inhibition Assay | IC50=0.2073 μM | SANGER | |||
| EW-3 | Growth Inhibition Assay | IC50=0.21718 μM | SANGER | |||
| KY821 | Growth Inhibition Assay | IC50=0.22045 μM | SANGER | |||
| CTB-1 | Growth Inhibition Assay | IC50=0.22228 μM | SANGER | |||
| TALL-1 | Growth Inhibition Assay | IC50=0.22921 μM | SANGER | |||
| NCI-H209 | Growth Inhibition Assay | IC50=0.22962 μM | SANGER | |||
| IST-MES1 | Growth Inhibition Assay | IC50=0.2351 μM | SANGER | |||
| DMS-114 | Growth Inhibition Assay | IC50=0.23993 μM | SANGER | |||
| SNU-C2B | Growth Inhibition Assay | IC50=0.24316 μM | SANGER | |||
| DG-75 | Growth Inhibition Assay | IC50=0.26195 μM | SANGER | |||
| D-336MG | Growth Inhibition Assay | IC50=0.26557 μM | SANGER | |||
| KMOE-2 | Growth Inhibition Assay | IC50=0.27538 μM | SANGER | |||
| NB1 | Growth Inhibition Assay | IC50=0.27926 μM | SANGER | |||
| RH-1 | Growth Inhibition Assay | IC50=0.27989 μM | SANGER | |||
| TE-1 | Growth Inhibition Assay | IC50=0.28479 μM | SANGER | |||
| RPMI-6666 | Growth Inhibition Assay | IC50=0.2884 μM | SANGER | |||
| TE-9 | Growth Inhibition Assay | IC50=0.28985 μM | SANGER | |||
| BT-474 | Growth Inhibition Assay | IC50=0.29334 μM | SANGER | |||
| COLO-668 | Growth Inhibition Assay | IC50=0.29429 μM | SANGER | |||
| NCI-H1581 | Growth Inhibition Assay | IC50=0.29689 μM | SANGER | |||
| MN-60 | Growth Inhibition Assay | IC50=0.29905 μM | SANGER | |||
| L-540 | Growth Inhibition Assay | IC50=0.30081 μM | SANGER | |||
| MOLT-13 | Growth Inhibition Assay | IC50=0.30183 μM | SANGER | |||
| NCI-SNU-16 | Growth Inhibition Assay | IC50=0.30205 μM | SANGER | |||
| NCI-H64 | Growth Inhibition Assay | IC50=0.30331 μM | SANGER | |||
| CP66-MEL | Growth Inhibition Assay | IC50=0.31415 μM | SANGER | |||
| NB69 | Growth Inhibition Assay | IC50=0.32188 μM | SANGER | |||
| NCI-H2196 | Growth Inhibition Assay | IC50=0.33036 μM | SANGER | |||
| MHH-CALL-2 | Growth Inhibition Assay | IC50=0.33468 μM | SANGER | |||
| HCC2157 | Growth Inhibition Assay | IC50=0.33954 μM | SANGER | |||
| LS-1034 | Growth Inhibition Assay | IC50=0.36388 μM | SANGER | |||
| BOKU | Growth Inhibition Assay | IC50=0.37199 μM | SANGER | |||
| GDM-1 | Growth Inhibition Assay | IC50=0.37234 μM | SANGER | |||
| TGW | Growth Inhibition Assay | IC50=0.37453 μM | SANGER | |||
| SNU-C1 | Growth Inhibition Assay | IC50=0.38215 μM | SANGER | |||
| LS-513 | Growth Inhibition Assay | IC50=0.39071 μM | SANGER | |||
| NOMO-1 | Growth Inhibition Assay | IC50=0.39971 μM | SANGER | |||
| MS-1 | Growth Inhibition Assay | IC50=0.40177 μM | SANGER | |||
| EKVX | Growth Inhibition Assay | IC50=0.44461 μM | SANGER | |||
| SK-MEL-2 | Growth Inhibition Assay | IC50=0.464 μM | SANGER | |||
| HCC1187 | Growth Inhibition Assay | IC50=0.46768 μM | SANGER | |||
| HCC2218 | Growth Inhibition Assay | IC50=0.47227 μM | SANGER | |||
| NCI-H187 | Growth Inhibition Assay | IC50=0.4784 μM | SANGER | |||
| DB | Growth Inhibition Assay | IC50=0.50055 μM | SANGER | |||
| NCI-H69 | Growth Inhibition Assay | IC50=0.50247 μM | SANGER | |||
| NCI-H1694 | Growth Inhibition Assay | IC50=0.50697 μM | SANGER | |||
| CA46 | Growth Inhibition Assay | IC50=0.50917 μM | SANGER | |||
| EHEB | Growth Inhibition Assay | IC50=0.51193 μM | SANGER | |||
| NCCIT | Growth Inhibition Assay | IC50=0.54321 μM | SANGER | |||
| NCI-H720 | Growth Inhibition Assay | IC50=0.54962 μM | SANGER | |||
| LU-134-A | Growth Inhibition Assay | IC50=0.55296 μM | SANGER | |||
| MC-CAR | Growth Inhibition Assay | IC50=0.55431 μM | SANGER | |||
| NEC8 | Growth Inhibition Assay | IC50=0.56172 μM | SANGER | |||
| NCI-H524 | Growth Inhibition Assay | IC50=0.58748 μM | SANGER | |||
| NCI-H1963 | Growth Inhibition Assay | IC50=0.6258 μM | SANGER | |||
| SU-DHL-1 | Growth Inhibition Assay | IC50=0.64526 μM | SANGER | |||
| NCI-H1522 | Growth Inhibition Assay | IC50=0.64883 μM | SANGER | |||
| NCI-H1155 | Growth Inhibition Assay | IC50=0.6535 μM | SANGER | |||
| D-263MG | Growth Inhibition Assay | IC50=0.66101 μM | SANGER | |||
| TE-6 | Growth Inhibition Assay | IC50=0.68104 μM | SANGER | |||
| NCI-H1417 | Growth Inhibition Assay | IC50=0.69711 μM | SANGER | |||
| NCI-H510A | Growth Inhibition Assay | IC50=0.77236 μM | SANGER | |||
| KARPAS-45 | Growth Inhibition Assay | IC50=0.78235 μM | SANGER | |||
| IST-SL1 | Growth Inhibition Assay | IC50=0.85825 μM | SANGER | |||
| COLO-824 | Growth Inhibition Assay | IC50=0.87835 μM | SANGER | |||
| NCI-H526 | Growth Inhibition Assay | IC50=0.90958 μM | SANGER | |||
| NCI-H719 | Growth Inhibition Assay | IC50=0.96657 μM | SANGER | |||
| SF539 | Growth Inhibition Assay | IC50=0.98396 μM | SANGER | |||
| NKM-1 | Growth Inhibition Assay | IC50=0.98663 μM | SANGER | |||
| A253 | Growth Inhibition Assay | IC50=0.98885 μM | SANGER | |||
| TE-441-T | Growth Inhibition Assay | IC50=1.0435 μM | SANGER | |||
| COR-L88 | Growth Inhibition Assay | IC50=1.11354 μM | SANGER | |||
| CPC-N | Growth Inhibition Assay | IC50=1.15888 μM | SANGER | |||
| NCI-H2107 | Growth Inhibition Assay | IC50=1.17261 μM | SANGER | |||
| ECC4 | Growth Inhibition Assay | IC50=1.18693 μM | SANGER | |||
| NCI-H2141 | Growth Inhibition Assay | IC50=1.19299 μM | SANGER | |||
| NCI-H1770 | Growth Inhibition Assay | IC50=1.32928 μM | SANGER | |||
| NCI-SNU-1 | Growth Inhibition Assay | IC50=1.3304 μM | SANGER | |||
| LU-165 | Growth Inhibition Assay | IC50=1.45467 μM | SANGER | |||
| Raji | Growth Inhibition Assay | IC50=1.47259 μM | SANGER | |||
| EW-24 | Growth Inhibition Assay | IC50=1.54619 μM | SANGER | |||
| KP-N-RT-BM-1 | Growth Inhibition Assay | IC50=1.56551 μM | SANGER | |||
| SK-MEL-1 | Growth Inhibition Assay | IC50=1.64877 μM | SANGER | |||
| SCLC-21H | Growth Inhibition Assay | IC50=1.66651 μM | SANGER | |||
| SIMA | Growth Inhibition Assay | IC50=1.68233 μM | SANGER | |||
| NCI-H1304 | Growth Inhibition Assay | IC50=1.70765 μM | SANGER | |||
| REH | Growth Inhibition Assay | IC50=1.71078 μM | SANGER | |||
| KP-N-YS | Growth Inhibition Assay | IC50=1.93551 μM | SANGER | |||
| DMS-153 | Growth Inhibition Assay | IC50=1.9759 μM | SANGER | |||
| NB10 | Growth Inhibition Assay | IC50=2.20878 μM | SANGER | |||
| NCI-H345 | Growth Inhibition Assay | IC50=2.61905 μM | SANGER | |||
| SK-N-FI | Growth Inhibition Assay | IC50=2.75381 μM | SANGER | |||
| NCI-H1092 | Growth Inhibition Assay | IC50=2.95394 μM | SANGER | |||
| KM-H2 | Growth Inhibition Assay | IC50=3.65396 μM | SANGER | |||
| KG-1 | Growth Inhibition Assay | IC50=3.71688 μM | SANGER | |||
| D-283MED | Growth Inhibition Assay | IC50=3.7587 μM | SANGER | |||
| LU-65 | Growth Inhibition Assay | IC50=4.11485 μM | SANGER | |||
| MSTO-211H | Growth Inhibition Assay | IC50=4.18898 μM | SANGER | |||
| NCI-H2081 | Growth Inhibition Assay | IC50=4.29174 μM | SANGER | |||
| D-247MG | Growth Inhibition Assay | IC50=4.43191 μM | SANGER | |||
| P31-FUJ | Growth Inhibition Assay | IC50=4.45019 μM | SANGER | |||
| DMS-79 | Growth Inhibition Assay | IC50=4.93183 μM | SANGER | |||
| KASUMI-1 | Growth Inhibition Assay | IC50=4.97509 μM | SANGER | |||
| JVM-3 | Growth Inhibition Assay | IC50=5.25014 μM | SANGER | |||
| LAN-6 | Growth Inhibition Assay | IC50=5.51736 μM | SANGER | |||
| DJM-1 | Growth Inhibition Assay | IC50=5.56029 μM | SANGER | |||
| JVM-2 | Growth Inhibition Assay | IC50=5.67092 μM | SANGER | |||
| U-87-MG | Growth Inhibition Assay | IC50=5.89964 μM | SANGER | |||
| SHP-77 | Growth Inhibition Assay | IC50=5.94928 μM | SANGER | |||
| RPMI-8866 | Growth Inhibition Assay | IC50=6.27013 μM | SANGER | |||
| ES7 | Growth Inhibition Assay | IC50=6.39924 μM | SANGER | |||
| WSU-NHL | Growth Inhibition Assay | IC50=6.51609 μM | SANGER | |||
| CAS-1 | Growth Inhibition Assay | IC50=7.09749 μM | SANGER | |||
| MHH-NB-11 | Growth Inhibition Assay | IC50=8.23152 μM | SANGER | |||
| ES5 | Growth Inhibition Assay | IC50=9.52286 μM | SANGER | |||
| HDLM-2 | Growth Inhibition Assay | IC50=9.95902 μM | SANGER | |||
| NCI-H2171 | Growth Inhibition Assay | IC50=10.5927 μM | SANGER | |||
| LNCaP-Clone-FGC | Growth Inhibition Assay | IC50=11.3718 μM | SANGER | |||
| NCI-H1395 | Growth Inhibition Assay | IC50=12.2699 μM | SANGER | |||
| NCI-H1436 | Growth Inhibition Assay | IC50=13.3195 μM | SANGER | |||
| RCC10RGB | Growth Inhibition Assay | IC50=13.3221 μM | SANGER | |||
| SW684 | Growth Inhibition Assay | IC50=13.9797 μM | SANGER | |||
| NCI-H1650 | Growth Inhibition Assay | IC50=15.0447 μM | SANGER | |||
| EW-13 | Growth Inhibition Assay | IC50=15.2021 μM | SANGER | |||
| EC-GI-10 | Growth Inhibition Assay | IC50=15.8644 μM | SANGER | |||
| CHP-126 | Growth Inhibition Assay | IC50=17.1918 μM | SANGER | |||
| NCI-H226 | Growth Inhibition Assay | IC50=18.0403 μM | SANGER | |||
| NCI-H1838 | Growth Inhibition Assay | IC50=20.1848 μM | SANGER | |||
| EW-11 | Growth Inhibition Assay | IC50=24.2718 μM | SANGER | |||
| EB2 | Growth Inhibition Assay | IC50=31.5318 μM | SANGER | |||
| D-502MG | Growth Inhibition Assay | IC50=43.9229 μM | SANGER | |||
| U-698-M | Growth Inhibition Assay | IC50=48.8671 μM | SANGER | |||
| ES3 | Growth Inhibition Assay | IC50=49.3893 μM | SANGER | |||
| PC3 | Function assay | Inhibition of PI3Kalpha in human PC3 cells expressing Akt1 S473D mutant assessed as phosphorylation of Akt Thr308 by immunoblotting, EC50 = 1.8 μM. | 21322566 | |||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells | 29435139 | |||
| A673 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells | 29435139 | |||
| BT-37 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells | 29435139 | |||
| OHS-50 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells | 29435139 | |||
| Rh41 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells | 29435139 | |||
| SJ-GBM2 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells | 29435139 | |||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells | 29435139 | |||
| NB-EBc1 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells | 29435139 | |||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells | 29435139 | |||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells | 29435139 | |||
| Click to View More Cell Line Experimental Data | ||||||
Biological Activity
| Description | Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K. | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Targets |
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| In vitro | ||||
| In vitro | Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3] | |||
|---|---|---|---|---|
| Kinase Assay | mTORC1 and mTORC2 in Vitro Kinase Assays | |||
| To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting. | ||||
| Cell Research | Cell lines | MEFs | ||
| Concentrations | ~250 nM | |||
| Incubation Time | 4 days | |||
| Method | Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer. | |||
| Experimental Result Images | Methods | Biomarkers | Images | PMID |
| Western blot | ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236) p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1 Vinculin / p-S6 |
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28494858 | |
| Immunofluorescence | lipin-1 |
|
28347999 | |
| Growth inhibition assay | Cell viability |
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28757207 | |
| In Vivo | ||
| In vivo | Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2] | |
|---|---|---|
| Animal Research | Animal Models | U87MG xenograft model |
| Dosages | 20 mg/kg | |
| Administration | Administered via i.p. once daily | |
Chemical Information & Solubility
| Molecular Weight | 607.62 | Formula | C35H28F3N5O2 |
| CAS No. | 1222998-36-8 | SDF | Download Torin 1 SDF |
| Smiles | CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F | ||
| Storage (From the date of receipt) | |||
|
In vitro |
DMSO : 3 mg/mL ( (4.93 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.) Water : Insoluble Ethanol : Insoluble |
Molecular Weight Calculator |
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In vivo Add solvents to the product individually and in order. |
In vivo Formulation Calculator |
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Tech Support
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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Frequently Asked Questions
Question 1:
How to re-constitute the inhibitor for i.p. administration?
Answer:
S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.
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