Torin 1

For research use only.

Catalog No.S2827

116 publications

Torin 1 Chemical Structure

CAS No. 1222998-36-8

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

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Selleck's Torin 1 has been cited by 116 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 NUTNbGRKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFz2U3VKSzVyPUCuNFAxOzB4IN88US=> M2DIeHNCVkeHUh?=
OCUB-M NX;aenRKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnXOTWM2OD1yLkCwNVk1KM7:TR?= MXHTRW5ITVJ?
DOHH-2 NV7qVWJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7LSmNxUUN3ME2wMlAxOjJ3IN88US=> NH7PPY1USU6JRWK=
NOS-1 NXrxWVlLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTBwMECyOFIh|ryP M2r3UnNCVkeHUh?=
L-363 NXfldldWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHMdHlKUUN3ME2wMlAxOjh2IN88US=> NFfT[XBUSU6JRWK=
RL95-2 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTBwMECzNkDPxE1? M{n0eXNCVkeHUh?=
BL-70 M2D1Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU[3fY1SUUN3ME2wMlAxOzN5IN88US=> NIPCSm5USU6JRWK=
TGBC1TKB NWrhR4pET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLzTWM2OD1yLkCwN|c6KM7:TR?= NFvKcnpUSU6JRWK=
697 MoTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7Gc4M1UUN3ME2wMlAxPjN2IN88US=> M1\pWXNCVkeHUh?=
RPMI-8226 NELJO3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDyTYxKSzVyPUCuNFA3PTFizszN MXvTRW5ITVJ?
BL-41 NFXP[oVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVX3b|NOUUN3ME2wMlAxPjl4IN88US=> M{TRcHNCVkeHUh?=
MRK-nu-1 M4jsPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIjy[mJKSzVyPUCuNFA4ODdizszN NF7wdoVUSU6JRWK=
EW-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4frSWlEPTB;MD6wNFc1QSEQvF2= NUT1eWFlW0GQR1XS
EVSA-T NYnabJVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXCRWdKSzVyPUCuNFA5QTJizszN NGfvWlFUSU6JRWK=
HUTU-80 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTBwMEC5Nlkh|ryP MkTCV2FPT0WU
VA-ES-BJ NX7odFVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2qzPWlEPTB;MD6wNFk5PCEQvF2= MmLDV2FPT0WU
GCIY NHnmd|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVi2UGRLUUN3ME2wMlAyODB5IN88US=> NYixW3hHW0GQR1XS
ES6 NEHRb4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTBwMEGwPFMh|ryP MkDYV2FPT0WU
NALM-6 NH[yZWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PMUWlEPTB;MD6wNVExOiEQvF2= NI\Q[GNUSU6JRWK=
LB996-RCC MlzES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXETWM2OD1yLkCxNVM4KM7:TR?= NUDXUVVKW0GQR1XS
EW-16 NX;icnl4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{TlO2lEPTB;MD6wNVE6OiEQvF2= NImwb4lUSU6JRWK=
KALS-1 NV3Ed2FLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7JZ|E6UUN3ME2wMlAyOTl6IN88US=> MkTEV2FPT0WU
OPM-2 NFrtR3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnOUmtSUUN3ME2wMlAyOjlizszN NHWyVmdUSU6JRWK=
KU812 NYrDcZNkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMEGyPVYh|ryP NV\lZZhqW0GQR1XS
A4-Fuk NXj6VnZ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofrTWM2OD1yLkCxN|E6KM7:TR?= MkTJV2FPT0WU
KGN M{jreGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3nVOWlEPTB;MD6wNVM2QSEQvF2= NWjYd|F2W0GQR1XS
KS-1 NHX5PYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDsUlNYUUN3ME2wMlAyOzl{IN88US=> MkLuV2FPT0WU
no-11 NG\sNYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;ETWM2OD1yLkCxN|k2KM7:TR?= NWHOTmdnW0GQR1XS
PSN1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnS[5g3UUN3ME2wMlAyPDB7IN88US=> MmjEV2FPT0WU
SK-LMS-1 NGTKOVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTBwMEG0NVMh|ryP NVfFZpRtW0GQR1XS
LS-411N M3PaV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2ftcGlEPTB;MD6wNVQ{QSEQvF2= NVLoOZFEW0GQR1XS
HT-144 MoH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPlcFJLUUN3ME2wMlAyPDVzIN88US=> M4DlWHNCVkeHUh?=
ACN MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlj2TWM2OD1yLkCxOUDPxE1? MWLTRW5ITVJ?
RKO MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVSzdIp[UUN3ME2wMlAyPTN|IN88US=> NEnoPIFUSU6JRWK=
CMK MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTMSmQ5UUN3ME2wMlAyPTR|IN88US=> MWnTRW5ITVJ?
GB-1 NGjZe5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4izb2lEPTB;MD6wNVU2OyEQvF2= MmXLV2FPT0WU
KE-37 NWe5T5FZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XaUWlEPTB;MD6wNVU4OyEQvF2= M3GzWnNCVkeHUh?=
no-10 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\kcWlEPTB;MD6wNVU5PCEQvF2= MnPJV2FPT0WU
MOLT-16 MnrOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTBwMEG2NlIh|ryP NVTjNohlW0GQR1XS
HEL NVOxcFR{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2raXmlEPTB;MD6wNVY1KM7:TR?= M3Wxc3NCVkeHUh?=
NCI-H1355 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLRTWM2OD1yLkCxOlQ3KM7:TR?= NHLLeFdUSU6JRWK=
IST-MEL1 MlnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljITWM2OD1yLkCxO|c1KM7:TR?= M4XlfHNCVkeHUh?=
MHH-PREB-1 M4DOfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTBwMEG4NVgh|ryP MkTZV2FPT0WU
ES8 NX;vTYZiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDqTWM2OD1yLkCxPFQ1KM7:TR?= NV;wXnZWW0GQR1XS
LC-2-ad MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LifWlEPTB;MD6wNlE{PyEQvF2= MnXiV2FPT0WU
LAMA-84 M3PLbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;oXVVKSzVyPUCuNFIyPjdizszN M2fLO3NCVkeHUh?=
BV-173 MkTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIO2ZWpKSzVyPUCuNFIzODNizszN NGniZVBUSU6JRWK=
SW872 NEnBeIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXx[3hKSzVyPUCuNFIzPDdizszN MkjaV2FPT0WU
SK-UT-1 Ml3MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\LemZKSzVyPUCuNFIzPjFizszN MnzmV2FPT0WU
OS-RC-2 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTBwMEKzNlMh|ryP NEDNVplUSU6JRWK=
NCI-H747 M4jwPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PnNWlEPTB;MD6wNlM{KM7:TR?= MXnTRW5ITVJ?
MZ2-MEL MlXLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;1SWlEPTB;MD6wNlM{PyEQvF2= MYHTRW5ITVJ?
TE-11 MmH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjRTWM2OD1yLkCyN|czKM7:TR?= NH7LSFdUSU6JRWK=
KURAMOCHI NYHFdVZtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTBwMEK0NVQh|ryP NVHxV|B4W0GQR1XS
HD-MY-Z NFjsdlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\KPWlEPTB;MD6wNlQzPiEQvF2= MnHkV2FPT0WU
GI-1 NHnxXnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\FSWlEPTB;MD6wNlQ3QSEQvF2= MlezV2FPT0WU
LB1047-RCC MlvVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHX3UotKSzVyPUCuNFI1QDhizszN MX7TRW5ITVJ?
TE-12 MoWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\pSHBKSzVyPUCuNFI2PSEQvF2= Mn\JV2FPT0WU
MZ7-mel MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHmxepZKSzVyPUCuNFI3ODlizszN NV\6[FRnW0GQR1XS
MMAC-SF NWqxNGRWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTBwMEK2N|Mh|ryP M4DXWnNCVkeHUh?=
A101D M4i2fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTBwMEK2OUDPxE1? NI\tN|hUSU6JRWK=
GR-ST MkWwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\0TWM2OD1yLkCyOlU2KM7:TR?= M4fiS3NCVkeHUh?=
MC116 Mk\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTBwMEK2PEDPxE1? NFjYeJBUSU6JRWK=
Ramos-2G6-4C10 MkXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nBOGlEPTB;MD6wNlc2KM7:TR?= MnvHV2FPT0WU
ATN-1 M1niSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLId3JPUUN3ME2wMlAzPzh7IN88US=> NGDmRVNUSU6JRWK=
MFH-ino MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PncWlEPTB;MD6wNlgyOiEQvF2= NHn4[YNUSU6JRWK=
DEL NVHMfpBoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHKUZFKSzVyPUCuNFI5OTVizszN M{Xt[3NCVkeHUh?=
OCI-AML2 MnLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonjTWM2OD1yLkCyPFcyKM7:TR?= NF6zVGNUSU6JRWK=
LXF-289 Mlz2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\uV2lEPTB;MD6wNlg4OiEQvF2= M33oU3NCVkeHUh?=
IA-LM MmDjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfmdnRKSzVyPUCuNFI6OTdizszN MVXTRW5ITVJ?
EoL-1-cell NGjDbXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfXWY9yUUN3ME2wMlAzQTl4IN88US=> NEPCTI1USU6JRWK=
OVCAR-4 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFTyZmxKSzVyPUCuNFMxQTRizszN NFfXbZlUSU6JRWK=
ES1 MoLQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTBwMEOwPVch|ryP NGD3N5VUSU6JRWK=
CESS NWDHeZdFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnT1TWM2OD1yLkCzNFk6KM7:TR?= NHz2VmZUSU6JRWK=
GAK NEHoe3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zsdGlEPTB;MD6wN|E5PiEQvF2= NGCxR4dUSU6JRWK=
NTERA-S-cl-D1 Mk\yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPo[5VKSzVyPUCuNFMzOjZizszN NFHvXFFUSU6JRWK=
TE-10 NF\rV5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFyyZXdKSzVyPUCuNFM{OTFizszN Ml;YV2FPT0WU
CCRF-CEM NULXeHVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWK2WJZIUUN3ME2wMlA{OzdzIN88US=> MUPTRW5ITVJ?
NMC-G1 NHX0TnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTBwMEO0OFgh|ryP MUfTRW5ITVJ?
HOP-62 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\me4hKSzVyPUCuNFM2PTZizszN NXXm[JV2W0GQR1XS
SH-4 MnjDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTBwMEO1PFIh|ryP MorPV2FPT0WU
NCI-H1648 Mof2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIDjOZpKSzVyPUCuNFM3OjJizszN MVjTRW5ITVJ?
EB-3 M{X6SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoD4TWM2OD1yLkCzOlQ{KM7:TR?= M1rDVHNCVkeHUh?=
BB65-RCC MoDOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rSZWlEPTB;MD6wN|Y6OSEQvF2= NHnKbW9USU6JRWK=
BE-13 Mln5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWraW2JxUUN3ME2wMlA{PzV7IN88US=> MULTRW5ITVJ?
CP67-MEL MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\QOWlEPTB;MD6wN|c2QSEQvF2= MoDiV2FPT0WU
SW954 M{TjUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX7TT5VIUUN3ME2wMlA{PzlizszN M4jDZXNCVkeHUh?=
TE-5 NGjsVZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoC2TWM2OD1yLkCzPFU1KM7:TR?= MmG2V2FPT0WU
HCE-4 M330O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;xbWNKSzVyPUCuNFM5PTVizszN NWXFVm9zW0GQR1XS
LC-1F NV\MTXFET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DuPWlEPTB;MD6wN|k5QSEQvF2= NWfXeoJWW0GQR1XS
TK10 M4fjfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGi1VGdKSzVyPUCuNFQxOTlizszN MWjTRW5ITVJ?
SJSA-1 M2fuOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXNToVKSzVyPUCuNFQyQDhizszN M1Sw[3NCVkeHUh?=
Daudi NGnhT41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LkPGlEPTB;MD6wOFIzPSEQvF2= NInSW4FUSU6JRWK=
SW982 M1TCeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnOO3JKSzVyPUCuNFQzPDZizszN M3nHSHNCVkeHUh?=
SF126 M2XwOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTBwMESzOlEh|ryP MonZV2FPT0WU
TE-15 M2PiSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;U[4JkUUN3ME2wMlA1PDN3IN88US=> MUDTRW5ITVJ?
COLO-684 NYrjXmtZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWX2N|FXUUN3ME2wMlA1PTZzIN88US=> MWjTRW5ITVJ?
8-MG-BA NFPlfYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLNPW9KSzVyPUCuNFQ3OjlizszN MWHTRW5ITVJ?
NB13 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlK0TWM2OD1yLkC0O|c3KM7:TR?= NVn4W3N3W0GQR1XS
HCC1599 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXzTWM2OD1yLkC0PFc6KM7:TR?= NUfaOWVPW0GQR1XS
SK-PN-DW NUDMe5JQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFSzUFJKSzVyPUCuNFQ6PDlizszN M1jaV3NCVkeHUh?=
ALL-PO MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3radmlEPTB;MD6wOFk2PiEQvF2= NVLuW2twW0GQR1XS
NB6 MoPwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\ITWM2OD1yLkC1NlcyKM7:TR?= NFztcY9USU6JRWK=
IST-SL2 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHFTWM2OD1yLkC1N|YzKM7:TR?= NVr3Upl[W0GQR1XS
BB30-HNC NVzDbJVtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjSTYhPUUN3ME2wMlA2PzNizszN MoLmV2FPT0WU
SW962 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTBwMEW3OlMh|ryP M13QTnNCVkeHUh?=
TGBC24TKB NVTSWVBwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HWXWlEPTB;MD6wOVc6QSEQvF2= Mn3jV2FPT0WU
KINGS-1 M4XVU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrNc5VnUUN3ME2wMlA2QDB|IN88US=> NV\hd2hPW0GQR1XS
HC-1 M4W2[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDoXW5KSzVyPUCuNFU5OjdizszN NISwc2xUSU6JRWK=
RS4-11 NYHkc|B5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTBwMEW5N|gh|ryP MmOyV2FPT0WU
LS-123 M4TSb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTBwMEW5OFIh|ryP MkD1V2FPT0WU
A3-KAW NXH3cZdWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHSUW5KSzVyPUCuNFYzOjVizszN M4CydnNCVkeHUh?=
SNB75 NEfEWmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPFTWM2OD1yLkC2N|QyKM7:TR?= M1TyVXNCVkeHUh?=
SR NEO2OJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvpSmRNUUN3ME2wMlA3Ozd2IN88US=> NIH0THZUSU6JRWK=
LOUCY NFT3OVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zGPGlEPTB;MD6wOlM6PCEQvF2= MWXTRW5ITVJ?
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NCI-H2141 NV;DVGxjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTFwMUmyPVkh|ryP M2r4PHNCVkeHUh?=
NCI-H1770 NES4R41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{HnXmlEPTB;MT6zNlkzQCEQvF2= NEP6RmdUSU6JRWK=
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Raji NUTJTlRNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33kSmlEPTB;MT60O|I2QSEQvF2= MlfTV2FPT0WU
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SK-MEL-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{S5RmlEPTB;MT62OFg4PyEQvF2= NYfpbpBlW0GQR1XS
SCLC-21H NYnUO|R3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXWOJQ5UUN3ME2xMlY3PjVzIN88US=> MoTmV2FPT0WU
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NCI-H1304 M{PUemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXxTWM2OD1zLkewO|Y2KM7:TR?= MlG2V2FPT0WU
REH Mon3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXpRmI{UUN3ME2xMlcyODd6IN88US=> MWnTRW5ITVJ?
KP-N-YS NGrP[WtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoT4TWM2OD1zLkmzOVUyKM7:TR?= MYDTRW5ITVJ?
DMS-153 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3u3dWlEPTB;MT65O|U6KM7:TR?= Mk\0V2FPT0WU
NB10 NX6yNoxpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;GZ2lEPTB;Mj6yNFg4QCEQvF2= M1TqWHNCVkeHUh?=
NCI-H345 M2\0[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHuxOHRKSzVyPUKuOlE6ODVizszN M2\DcXNCVkeHUh?=
SK-N-FI MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXqcpFKSzVyPUKuO|U{QDFizszN NGfIfXZUSU6JRWK=
NCI-H1092 NXmz[GJ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTJwOUWzPVQh|ryP M{Oyb3NCVkeHUh?=
KM-H2 NXjwT|lLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFn6fohKSzVyPUOuOlU{QTZizszN NVOzR3B7W0GQR1XS
KG-1 NVjMeYVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXVXXVrUUN3ME2zMlcyPjh6IN88US=> M2HORXNCVkeHUh?=
D-283MED NY[ybG9ST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXGUFNtUUN3ME2zMlc2QDdizszN MVvTRW5ITVJ?
LU-65 NGHaT21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3P5R2lEPTB;ND6xNVQ5PSEQvF2= M3zKVHNCVkeHUh?=
MSTO-211H MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljlTWM2OD12LkG4PFk5KM7:TR?= Mn3TV2FPT0WU
NCI-H2081 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlSwTWM2OD12LkK5NVc1KM7:TR?= NEW4VXpUSU6JRWK=
D-247MG NV3ZT4pTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1m1bWlEPTB;ND60N|E6OSEQvF2= Ml20V2FPT0WU
P31-FUJ NHjnUFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInyUJdKSzVyPUSuOFUxOTlizszN NYPYU211W0GQR1XS
DMS-79 NHzjTlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzzTWM2OD12LkmzNVg{KM7:TR?= NGrTZZRUSU6JRWK=
KASUMI-1 MmL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;uVYNOUUN3ME20Mlk4PTB7IN88US=> NFu2VHpUSU6JRWK=
JVM-3 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHUTWM2OD13LkK1NFE1KM7:TR?= M1HLd3NCVkeHUh?=
LAN-6 NGfXUXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnjKTWM2OD13LkWxO|M3KM7:TR?= MlraV2FPT0WU
DJM-1 NH3iW4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;VT3dKSzVyPUWuOVYxOjlizszN MnnOV2FPT0WU
JVM-2 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXmd4o1UUN3ME21MlY4ODl{IN88US=> M3rrcHNCVkeHUh?=
U-87-MG NH7JOVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTTTWM2OD13Lki5PVY1KM7:TR?= NVnVd45HW0GQR1XS
SHP-77 MnOzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DTV2lEPTB;NT65OFkzQCEQvF2= Moi3V2FPT0WU
RPMI-8866 Mo\JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTZwMkewNVMh|ryP M12xe3NCVkeHUh?=
ES7 NGrXOW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXlTWM2OD14LkO5PVI1KM7:TR?= M{Pxb3NCVkeHUh?=
WSU-NHL NF;WXZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;SXWlEPTB;Nj61NVYxQSEQvF2= MWTTRW5ITVJ?
CAS-1 NVLiTVVmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGThWGxKSzVyPUeuNFk4PDlizszN MkLmV2FPT0WU
MHH-NB-11 NGq3fW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRThwMkOxOVIh|ryP MmHEV2FPT0WU
ES5 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHNTWM2OD17LkWyNlg3KM7:TR?= M2rhZ3NCVkeHUh?=
HDLM-2 MkfUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3j4fmlEPTB;OT65OVkxOiEQvF2= MkfJV2FPT0WU
NCI-H2171 NYnDXGRCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHKNXZpUUN3ME2xNE42QTJ5IN88US=> NWXRcI9KW0GQR1XS
LNCaP-Clone-FGC NVn5PVI1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLtTWM2OD1zMT6zO|E5KM7:TR?= MofnV2FPT0WU
NCI-H1395 NUfQUnNRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlfhTWM2OD1zMj6yOlk6KM7:TR?= MlTMV2FPT0WU
NCI-H1436 NHH1W5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfrWo5KSzVyPUGzMlMyQTVizszN MXvTRW5ITVJ?
RCC10RGB M3yyWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTF|LkOyNlEh|ryP MnPpV2FPT0WU
SW684 NWPX[I95T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWj1TnlVUUN3ME2xN{46Pzl5IN88US=> MWDTRW5ITVJ?
NCI-H1650 NF7NXVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLvbVBKSzVyPUG1MlA1PDdizszN M{f3enNCVkeHUh?=
EW-13 M{[zdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVy0PFdZUUN3ME2xOU4zODJzIN88US=> MoPmV2FPT0WU
EC-GI-10 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1P1U2lEPTB;MUWuPFY1PCEQvF2= Mn7xV2FPT0WU
CHP-126 NYfZXnJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DMWmlEPTB;MUeuNVkyQCEQvF2= M3XTOXNCVkeHUh?=
NCI-H226 NELOOJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkPITWM2OD1zOD6wOFA{KM7:TR?= MoXiV2FPT0WU
NCI-H1838 MkXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33FfmlEPTB;MkCuNVg1QCEQvF2= M3nHXnNCVkeHUh?=
EW-11 NXHvNFlPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2roS2lEPTB;MkSuNlcyQCEQvF2= M{PSS3NCVkeHUh?=
EB2 M2HNTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPtbHVKSzVyPUOxMlU{OThizszN NE[0SXRUSU6JRWK=
D-502MG MkO0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIT0WZVKSzVyPUSzMlkzOjlizszN NHfuWIVUSU6JRWK=
U-698-M MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfPe4lxUUN3ME20PE45PjdzIN88US=> M3v4c3NCVkeHUh?=
ES3 NEPYUoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LoN2lEPTB;NEmuN|g6OyEQvF2= NGG3e3hUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol

Kinase Assay:[1]
- Collapse

mTORC1 and mTORC2 in Vitro Kinase Assays:

To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research:[1]
- Collapse
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: U87MG xenograft model
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
20% NMP+50% PEG 400
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage powder
in solvent
Synonyms N/A
Smiles CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to re-constitute the inhibitor for i.p. administration?

  • Answer:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

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