Torin 1

Catalog No.S2827

Torin 1 Chemical Structure

Molecular Weight(MW): 607.62

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

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Cited by 94 Publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 MknNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfvTWM2OD1yLkCwNFMxPiEQvF2= Ml7RV2FPT0WU
OCUB-M NFHGb5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTBwMECxPVQh|ryP NVyz[nAzW0GQR1XS
DOHH-2 NW\yWJd6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn:3TWM2OD1yLkCwNlI2KM7:TR?= NHvCc|hUSU6JRWK=
NOS-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3BXFZ1UUN3ME2wMlAxOjR{IN88US=> MlG1V2FPT0WU
L-363 NF\ad3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTBwMECyPFQh|ryP NGrvTYpUSU6JRWK=
RL95-2 NYfqelFtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPsTWM2OD1yLkCwN|Ih|ryP NX\odYxbW0GQR1XS
BL-70 NYTVeGN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfqN|BvUUN3ME2wMlAxOzN5IN88US=> Moj0V2FPT0WU
TGBC1TKB MmHxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojuTWM2OD1yLkCwN|c6KM7:TR?= MWTTRW5ITVJ?
697 Mkf4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTBwMEC2N|Qh|ryP NEm1T|dUSU6JRWK=
RPMI-8226 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrpc5poUUN3ME2wMlAxPjVzIN88US=> MXnTRW5ITVJ?
BL-41 NF3HeGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTBwMEC2PVYh|ryP MYHTRW5ITVJ?
MRK-nu-1 M{H6[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWeyU3lRUUN3ME2wMlAxPzB5IN88US=> MX3TRW5ITVJ?
EW-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnZOZF7UUN3ME2wMlAxPzR7IN88US=> Mm[0V2FPT0WU
EVSA-T NXzMTnh2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXRTWM2OD1yLkCwPFkzKM7:TR?= Mny1V2FPT0WU
HUTU-80 NGDyWHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTBwMEC5Nlkh|ryP MmXRV2FPT0WU
VA-ES-BJ MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUXMXHVCUUN3ME2wMlAxQTh2IN88US=> MWDTRW5ITVJ?
GCIY NVG3TI9XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHy0eYtKSzVyPUCuNFExODdizszN M{nGcnNCVkeHUh?=
ES6 NXvSWnRZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXy3[HFOUUN3ME2wMlAyODh|IN88US=> NHHIR5JUSU6JRWK=
NALM-6 NIjtRmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PyWWlEPTB;MD6wNVExOiEQvF2= M{HpRnNCVkeHUh?=
LB996-RCC M2mwXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zHb2lEPTB;MD6wNVE{PyEQvF2= MknJV2FPT0WU
EW-16 NIrhTHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTBwMEGxPVIh|ryP MWTTRW5ITVJ?
KALS-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTxTWM2OD1yLkCxNVk5KM7:TR?= NVu4RlVyW0GQR1XS
OPM-2 Mnq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjsS5BKSzVyPUCuNFEzQSEQvF2= MUDTRW5ITVJ?
KU812 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXqXplHUUN3ME2wMlAyOjl4IN88US=> MYnTRW5ITVJ?
A4-Fuk MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PuUGlEPTB;MD6wNVMyQSEQvF2= MlXJV2FPT0WU
KGN NFjsTZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXGXZRSUUN3ME2wMlAyOzV7IN88US=> NX3kTVREW0GQR1XS
KS-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXiTIVKSzVyPUCuNFE{QTJizszN MXzTRW5ITVJ?
no-11 NFrxZ2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTBwMEGzPVUh|ryP MUnTRW5ITVJ?
PSN1 NYH2WXd2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvmeWNKSzVyPUCuNFE1ODlizszN MYXTRW5ITVJ?
SK-LMS-1 NIjDZ3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXoTWM2OD1yLkCxOFE{KM7:TR?= MWLTRW5ITVJ?
LS-411N NV\oRlBMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTBwMEG0N|kh|ryP Mn7mV2FPT0WU
HT-144 M{TXcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml33TWM2OD1yLkCxOFUyKM7:TR?= MnXvV2FPT0WU
ACN MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfHToNKSzVyPUCuNFE2KM7:TR?= NWPUT4RyW0GQR1XS
RKO NXnxUmZVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NImyWItKSzVyPUCuNFE2OzNizszN NYjUem5pW0GQR1XS
CMK MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjUWpRKSzVyPUCuNFE2PDNizszN NEDsXVRUSU6JRWK=
GB-1 Ml7tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXW2fpJoUUN3ME2wMlAyPTV|IN88US=> M{DhV3NCVkeHUh?=
KE-37 NH\sVHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTBTWM2OD1yLkCxOVc{KM7:TR?= NWHFVYpLW0GQR1XS
no-10 NWPyU4tZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfW[Jk1UUN3ME2wMlAyPTh2IN88US=> NX\pPVA2W0GQR1XS
MOLT-16 NELnd4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDWRZhKSzVyPUCuNFE3OjJizszN MX;TRW5ITVJ?
HEL NFPkWm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnj2TWM2OD1yLkCxOlQh|ryP NXm5VXM6W0GQR1XS
NCI-H1355 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLHb45KSzVyPUCuNFE3PDZizszN Mly4V2FPT0WU
IST-MEL1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTBwMEG3O|Qh|ryP M{nu[HNCVkeHUh?=
MHH-PREB-1 MmPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTBwMEG4NVgh|ryP MU\TRW5ITVJ?
ES8 Mne5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTBwMEG4OFQh|ryP NEH5NFhUSU6JRWK=
LC-2-ad Mn2yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDRTWM2OD1yLkCyNVM4KM7:TR?= M3rLVHNCVkeHUh?=
LAMA-84 MmrPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUi0cGNtUUN3ME2wMlAzOTZ5IN88US=> NXWxe3A6W0GQR1XS
BV-173 NYP6fWYxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTifJFlUUN3ME2wMlAzOjB|IN88US=> M37xVHNCVkeHUh?=
SW872 NWThVIZVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrKNlZKSzVyPUCuNFIzPDdizszN M{LQVXNCVkeHUh?=
SK-UT-1 M1zaWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7vTWM2OD1yLkCyNlYyKM7:TR?= NX;mXndYW0GQR1XS
OS-RC-2 MlP0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTBwMEKzNlMh|ryP NEDUVm1USU6JRWK=
NCI-H747 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTBwMEKzN{DPxE1? NUjSTnh4W0GQR1XS
MZ2-MEL MmfyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTXTWM2OD1yLkCyN|M4KM7:TR?= NVnUZWt2W0GQR1XS
TE-11 NF\GTpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGC0enZKSzVyPUCuNFI{PzJizszN NWfmT3VMW0GQR1XS
KURAMOCHI MnXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HqSWlEPTB;MD6wNlQyPCEQvF2= M2TqfXNCVkeHUh?=
HD-MY-Z NXHX[3p2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjiUZZ{UUN3ME2wMlAzPDJ4IN88US=> NWHUZmQ{W0GQR1XS
GI-1 NEPse2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDZTZprUUN3ME2wMlAzPDZ7IN88US=> M2DEV3NCVkeHUh?=
LB1047-RCC MlHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HkdWlEPTB;MD6wNlQ5QCEQvF2= MWTTRW5ITVJ?
TE-12 NWHPTYwyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTBwMEK1OUDPxE1? NF;PRlFUSU6JRWK=
MZ7-mel NGPMcGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfT[GJqUUN3ME2wMlAzPjB7IN88US=> MknQV2FPT0WU
MMAC-SF NIfzenFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfoU4pKSzVyPUCuNFI3OzNizszN MX\TRW5ITVJ?
A101D NWrNepdMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHzTWM2OD1yLkCyOlUh|ryP MkLaV2FPT0WU
GR-ST NV3hWJF1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVflUHVZUUN3ME2wMlAzPjV3IN88US=> M3HFOnNCVkeHUh?=
MC116 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXnSmZKSzVyPUCuNFI3QCEQvF2= NXH5O21zW0GQR1XS
Ramos-2G6-4C10 NUfIdpJpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTBwMEK3OUDPxE1? M4LnN3NCVkeHUh?=
ATN-1 MkKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmTBTWM2OD1yLkCyO|g6KM7:TR?= MYjTRW5ITVJ?
MFH-ino NFXHZVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlf5TWM2OD1yLkCyPFEzKM7:TR?= NXvFdHFmW0GQR1XS
DEL MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTBwMEK4NVUh|ryP MoHEV2FPT0WU
OCI-AML2 NHzzdZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPVWIpnUUN3ME2wMlAzQDdzIN88US=> MnfPV2FPT0WU
LXF-289 NEfY[XlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2PKOGlEPTB;MD6wNlg4OiEQvF2= MlfxV2FPT0WU
IA-LM MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\ZepVKSzVyPUCuNFI6OTdizszN NIDKenpUSU6JRWK=
EoL-1-cell MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nZdGlEPTB;MD6wNlk6PiEQvF2= NVnKVJllW0GQR1XS
OVCAR-4 NHG3ZnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTTfmpKSzVyPUCuNFMxQTRizszN MYnTRW5ITVJ?
ES1 NUPaVXBiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXicYNKSzVyPUCuNFMxQTdizszN M1e0PHNCVkeHUh?=
CESS NYjjWmNsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHQfmhKSzVyPUCuNFMxQTlizszN NYrEWmtKW0GQR1XS
GAK MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlS4TWM2OD1yLkCzNVg3KM7:TR?= NYfjT4xFW0GQR1XS
NTERA-S-cl-D1 Ml3CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nTO2lEPTB;MD6wN|IzPiEQvF2= M3zzVHNCVkeHUh?=
TE-10 MmrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjv[WtKSzVyPUCuNFM{OTFizszN NF62VIpUSU6JRWK=
CCRF-CEM MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTBwMEOzO|Eh|ryP MmXMV2FPT0WU
NMC-G1 Mkf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXJTWM2OD1yLkCzOFQ5KM7:TR?= MV3TRW5ITVJ?
HOP-62 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFy2blRKSzVyPUCuNFM2PTZizszN MlrtV2FPT0WU
SH-4 M2rQZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHX0VXhKSzVyPUCuNFM2QDJizszN NVrKNmlVW0GQR1XS
NCI-H1648 MnjTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWLufW1WUUN3ME2wMlA{PjJ{IN88US=> M2Hh[nNCVkeHUh?=
EB-3 M4X0fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTBwMEO2OFMh|ryP NVTPb3BvW0GQR1XS
BB65-RCC NHTHOm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;PToJKSzVyPUCuNFM3QTFizszN NVXXPYxOW0GQR1XS
BE-13 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fGeWlEPTB;MD6wN|c2QSEQvF2= MXjTRW5ITVJ?
CP67-MEL M4rn[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jCO2lEPTB;MD6wN|c2QSEQvF2= MVfTRW5ITVJ?
SW954 MljwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVWze4tQUUN3ME2wMlA{PzlizszN NHq2OY1USU6JRWK=
TE-5 MnzMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{XZWGlEPTB;MD6wN|g2PCEQvF2= Mly2V2FPT0WU
HCE-4 Mnv5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTBwMEO4OVUh|ryP MV3TRW5ITVJ?
LC-1F M4HSXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLyTWM2OD1yLkCzPVg6KM7:TR?= NEfJcI9USU6JRWK=
TK10 M{HHSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;abIZKSzVyPUCuNFQxOTlizszN M1e1dXNCVkeHUh?=
SJSA-1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHOSXNFUUN3ME2wMlA1OTh6IN88US=> M4PVZ3NCVkeHUh?=
Daudi NVTzWm13T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnezTWM2OD1yLkC0NlI2KM7:TR?= NH7nTVNUSU6JRWK=
SW982 M1rscGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LzNWlEPTB;MD6wOFI1PiEQvF2= NVnWPJBtW0GQR1XS
SF126 Mle1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDYTWM2OD1yLkC0N|YyKM7:TR?= MkDTV2FPT0WU
TE-15 M2jJcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;kdYlDUUN3ME2wMlA1PDN3IN88US=> MYrTRW5ITVJ?
COLO-684 Mmj1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3SxOmlEPTB;MD6wOFU3OSEQvF2= M1vae3NCVkeHUh?=
8-MG-BA NFToOWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonRTWM2OD1yLkC0OlI6KM7:TR?= M{DqRnNCVkeHUh?=
NB13 M1[1dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfWbGVvUUN3ME2wMlA1Pzd4IN88US=> NX3IephTW0GQR1XS
HCC1599 NIXyV3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTBwMES4O|kh|ryP NXzTVpZ1W0GQR1XS
SK-PN-DW MnjJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml:3TWM2OD1yLkC0PVQ6KM7:TR?= M1;kZXNCVkeHUh?=
ALL-PO NV3OSXBsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTBwMES5OVYh|ryP M1rMbXNCVkeHUh?=
NB6 NVrac5AzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33yb2lEPTB;MD6wOVI4OSEQvF2= NF7xTFJUSU6JRWK=
IST-SL2 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTBwMEWzOlIh|ryP NXrh[ZAyW0GQR1XS
BB30-HNC MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFWwblFKSzVyPUCuNFU4OyEQvF2= NVPDN5NwW0GQR1XS
SW962 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTBwMEW3OlMh|ryP NEXVT3BUSU6JRWK=
TGBC24TKB M2nqSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTBwMEW3PVkh|ryP NVfGVZk6W0GQR1XS
KINGS-1 M1jBW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTPTWM2OD1yLkC1PFA{KM7:TR?= NFPCfplUSU6JRWK=
HC-1 MofYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFjlNGNKSzVyPUCuNFU5OjdizszN MUfTRW5ITVJ?
RS4-11 NIrUTo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fPVmlEPTB;MD6wOVk{QCEQvF2= M17aO3NCVkeHUh?=
LS-123 MmHmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXDfpdqUUN3ME2wMlA2QTR{IN88US=> NXPvVJVlW0GQR1XS
A3-KAW MnfxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvCTWM2OD1yLkC2NlI2KM7:TR?= NXXlRXZYW0GQR1XS
SNB75 NYHJOpNIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTBwME[zOFEh|ryP MUHTRW5ITVJ?
SR M{\GW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3yybmlEPTB;MD6wOlM4PCEQvF2= M4XTXnNCVkeHUh?=
LOUCY M4\ueWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mlf5TWM2OD1yLkC2N|k1KM7:TR?= M4HzUnNCVkeHUh?=
K5 M{HxXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XGUmlEPTB;MD6wOlQxOyEQvF2= MWrTRW5ITVJ?
Becker MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLSTWM2OD1yLkC2OFIh|ryP MUPTRW5ITVJ?
CTV-1 NWnHTIFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jrU2lEPTB;MD6wOlU1QCEQvF2= MULTRW5ITVJ?
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HCC2218 NGL4fHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7uTWM2OD1yLkS3NlI4KM7:TR?= NYPJTXZXW0GQR1XS
NCI-H187 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37BSmlEPTB;MD60O|g1KM7:TR?= M4rlVHNCVkeHUh?=
DB MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTBwNUCwOVUh|ryP NV\kZXpZW0GQR1XS
NCI-H69 MoLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HKd2lEPTB;MD61NFI1PyEQvF2= MU\TRW5ITVJ?
NCI-H1694 NVfwSHJUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml[zTWM2OD1yLkWwOlk4KM7:TR?= NVnHNlFKW0GQR1XS
CA46 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHFTWM2OD1yLkWwPVE4KM7:TR?= MlTyV2FPT0WU
EHEB M1:0Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGmzZ3BKSzVyPUCuOVEyQTNizszN MX3TRW5ITVJ?
NCCIT NWW3NGVoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTBwNUSzNlEh|ryP MnnYV2FPT0WU
NCI-H720 NEjic21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1O2NGlEPTB;MD61OFk3OiEQvF2= Mlv6V2FPT0WU
LU-134-A M1\0Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTWW5pbUUN3ME2wMlU2Ojl4IN88US=> MYLTRW5ITVJ?
MC-CAR NUO5[JBZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLlTWM2OD1yLkW1OFMyKM7:TR?= M{G3PXNCVkeHUh?=
NEC8 M1LxO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXkR2lUUUN3ME2wMlU3OTd{IN88US=> M2PINHNCVkeHUh?=
NCI-H524 NV3PO4xKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnoPYdKSzVyPUCuOVg4PDhizszN MXTTRW5ITVJ?
NCI-H1963 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LadWlEPTB;MD62NlU5KM7:TR?= M1\MVHNCVkeHUh?=
SU-DHL-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELOUY5KSzVyPUCuOlQ2OjZizszN MnLQV2FPT0WU
NCI-H1522 MmLES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjPb41JUUN3ME2wMlY1QDh|IN88US=> NXfuSm4zW0GQR1XS
NCI-H1155 Mnr3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXPTWM2OD1yLk[1N|Uh|ryP NWjaN|Z5W0GQR1XS
D-263MG NF:3dVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrzfZBKSzVyPUCuOlYyODFizszN M2rrUXNCVkeHUh?=
TE-6 M3LHO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXHTWM2OD1yLk[4NVA1KM7:TR?= M3PkSnNCVkeHUh?=
NCI-H1417 MnvOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTBwNkm3NVEh|ryP NXf2PYdPW0GQR1XS
NCI-H510A MmjVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HGV2lEPTB;MD63O|I{PiEQvF2= NUnHOXltW0GQR1XS
KARPAS-45 NH7WWYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGX3W5pKSzVyPUCuO|gzOzVizszN M{HDenNCVkeHUh?=
IST-SL1 MlTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLpTWM2OD1yLki1PFI2KM7:TR?= NXjxbYx1W0GQR1XS
COLO-824 M37DTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vrOWlEPTB;MD64O|g{PSEQvF2= M3H6fXNCVkeHUh?=
NCI-H526 NIHCXnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjTOWlbUUN3ME2wMlkxQTV6IN88US=> MVHTRW5ITVJ?
NCI-H719 M3jTZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTBwOU[2OVch|ryP MlPSV2FPT0WU
SF539 NIrBRpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrvb5NuUUN3ME2wMlk5Ozl4IN88US=> MXLTRW5ITVJ?
NKM-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3zbYhJUUN3ME2wMlk5PjZ|IN88US=> NHqzOYRUSU6JRWK=
A253 NHe3XFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnjYTWM2OD1yLkm4PFg2KM7:TR?= NFvrWZZUSU6JRWK=
TE-441-T MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPMXFlKSzVyPUGuNFQ{PSEQvF2= M1zF[nNCVkeHUh?=
COR-L88 NF3kXXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPWTWM2OD1zLkGxN|U1KM7:TR?= NXfUe3BUW0GQR1XS
CPC-N NFfUNZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfVR3k6UUN3ME2xMlE2QDh6IN88US=> NXnGdI9GW0GQR1XS
NCI-H2107 NVPhO|k6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nhSWlEPTB;MT6xO|I3OSEQvF2= MY\TRW5ITVJ?
ECC4 M1e1fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTFwMUi2PVMh|ryP MXvTRW5ITVJ?
NCI-H2141 NVS2R|l6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\Q[pFKSzVyPUGuNVkzQTlizszN M3nac3NCVkeHUh?=
NCI-H1770 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\UXYJKSzVyPUGuN|I6OjhizszN NWHkSXNJW0GQR1XS
NCI-SNU-1 NGDveZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TxV2lEPTB;MT6zN|A1KM7:TR?= NXz6NVU2W0GQR1XS
LU-165 M3L6VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTFwNEW0Olch|ryP NWq0N29kW0GQR1XS
Raji MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;lTWM2OD1zLkS3NlU6KM7:TR?= NY\ZXHlkW0GQR1XS
EW-24 NYXxPZZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HJS2lEPTB;MT61OFYyQSEQvF2= NVuwWJVDW0GQR1XS
KP-N-RT-BM-1 MkXmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXSd5dYUUN3ME2xMlU3PTVzIN88US=> MX7TRW5ITVJ?
SK-MEL-1 M4PUTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\OTWM2OD1zLk[0PFc4KM7:TR?= MVTTRW5ITVJ?
SCLC-21H MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2n1XGlEPTB;MT62OlY2OSEQvF2= MXfTRW5ITVJ?
SIMA Mke5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXzdoczUUN3ME2xMlY5OjN|IN88US=> NIm1cWNUSU6JRWK=
NCI-H1304 Mkj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXTc5hzUUN3ME2xMlcxPzZ3IN88US=> NXi2Xo9uW0GQR1XS
REH NEnMPI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTFwN{GwO|gh|ryP NHr5[mxUSU6JRWK=
KP-N-YS MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofETWM2OD1zLkmzOVUyKM7:TR?= NWrVOlNEW0GQR1XS
DMS-153 M4LIdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEf1UmdKSzVyPUGuPVc2QSEQvF2= MYnTRW5ITVJ?
NB10 NUHBW|RNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7UcWxKSzVyPUKuNlA5PzhizszN M{X6NnNCVkeHUh?=
NCI-H345 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrUTWM2OD1{Lk[xPVA2KM7:TR?= NIm0bVlUSU6JRWK=
SK-N-FI M4rXOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTJwN{WzPFEh|ryP NVzxW2NzW0GQR1XS
NCI-H1092 M2nHbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vuW2lEPTB;Mj65OVM6PCEQvF2= Mlr0V2FPT0WU
KM-H2 NXvXbHhtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrRZoRHUUN3ME2zMlY2Ozl4IN88US=> MkTuV2FPT0WU
KG-1 NX61OG9DT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfJe4xwUUN3ME2zMlcyPjh6IN88US=> NYjRWW43W0GQR1XS
D-283MED M4Wx[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPWfpZkUUN3ME2zMlc2QDdizszN NFPUU2tUSU6JRWK=
LU-65 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDYOXBKSzVyPUSuNVE1QDVizszN NXntPZBPW0GQR1XS
MSTO-211H MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTxUXQ5UUN3ME20MlE5QDl6IN88US=> NWrneYJZW0GQR1XS
NCI-H2081 Ml6xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTRwMkmxO|Qh|ryP MULTRW5ITVJ?
D-247MG Mn\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEX1RpRKSzVyPUSuOFMyQTFizszN NWG5O3djW0GQR1XS
P31-FUJ M1LKNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{jHemlEPTB;ND60OVAyQSEQvF2= NFfDS4xUSU6JRWK=
DMS-79 M4SyUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF62fJFKSzVyPUSuPVMyQDNizszN M1XzeXNCVkeHUh?=
KASUMI-1 Moj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3TR3FKSzVyPUSuPVc2ODlizszN NWCxXFZpW0GQR1XS
JVM-3 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jyeGlEPTB;NT6yOVAyPCEQvF2= MXPTRW5ITVJ?
LAN-6 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHvTWM2OD13LkWxO|M3KM7:TR?= NHrkdXhUSU6JRWK=
DJM-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkK3TWM2OD13LkW2NFI6KM7:TR?= NFnLTodUSU6JRWK=
JVM-2 MoqzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnyblZKSzVyPUWuOlcxQTJizszN NYTLXJBqW0GQR1XS
U-87-MG NYfac3F1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHDTWM2OD13Lki5PVY1KM7:TR?= NI\ZO3hUSU6JRWK=
SHP-77 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTVwOUS5Nlgh|ryP NXjRNZhIW0GQR1XS
RPMI-8866 NVLFOJFpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnOdoFNUUN3ME22MlI4ODF|IN88US=> NVnKbZJVW0GQR1XS
ES7 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PnR2lEPTB;Nj6zPVkzPCEQvF2= NYDEVlh6W0GQR1XS
WSU-NHL MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\rWlA6UUN3ME22MlUyPjB7IN88US=> NFrscFlUSU6JRWK=
CAS-1 NHraS4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrLXYNKSzVyPUeuNFk4PDlizszN MV3TRW5ITVJ?
MHH-NB-11 NWLJeo9RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRThwMkOxOVIh|ryP NHuzeXRUSU6JRWK=
ES5 NIXydZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTlwNUKyPFYh|ryP MWHTRW5ITVJ?
HDLM-2 NH:2[4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTlwOUW5NFIh|ryP NVrhPVl5W0GQR1XS
NCI-H2171 MmrDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7vTWM2OD1zMD61PVI4KM7:TR?= M16wSnNCVkeHUh?=
LNCaP-Clone-FGC M2Tsc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTFzLkO3NVgh|ryP MnzYV2FPT0WU
NCI-H1395 NVG5NmxQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLYTWM2OD1zMj6yOlk6KM7:TR?= M3q3PXNCVkeHUh?=
NCI-H1436 M13xbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTF|LkOxPVUh|ryP NXv3TYdUW0GQR1XS
RCC10RGB MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvFTWM2OD1zMz6zNlIyKM7:TR?= MVzTRW5ITVJ?
SW684 NVXlcGd{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\4XmRKSzVyPUGzMlk4QTdizszN MmPsV2FPT0WU
NCI-H1650 NV;RUHFFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTF3LkC0OFch|ryP MXnTRW5ITVJ?
EW-13 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mkf5TWM2OD1zNT6yNFIyKM7:TR?= M4PaN3NCVkeHUh?=
EC-GI-10 MnvFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTF3Lki2OFQh|ryP NUnB[WRYW0GQR1XS
CHP-126 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTF5LkG5NVgh|ryP MYLTRW5ITVJ?
NCI-H226 MojLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;iRYxKSzVyPUG4MlA1ODNizszN NWnhWlNCW0GQR1XS
NCI-H1838 Mn7DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTJyLkG4OFgh|ryP MkjxV2FPT0WU
EW-11 MkezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTJ2LkK3NVgh|ryP NILlUI5USU6JRWK=
EB2 Mn72S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTNzLkWzNVgh|ryP NVHlOoI{W0GQR1XS
D-502MG MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTR|LkmyNlkh|ryP M{nrdXNCVkeHUh?=
U-698-M NXTHOIx7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUe5[JVNUUN3ME20PE45PjdzIN88US=> MYDTRW5ITVJ?
ES3 MlvwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGmydJZKSzVyPUS5MlM5QTNizszN MmHJV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol

Kinase Assay:[1]
- Collapse

mTORC1 and mTORC2 in Vitro Kinase Assays:

To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research:[1]
- Collapse
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: U87MG xenograft model
  • Formulation: Torin1 powder is first dissolved at 25 mg/mL in 100% N-methyl-2-pyrrolidone and subsequently diluted 1:4 with sterile 50% PEG400 to a final concentration of 5 mg/mL
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
20% NMP+50% PEG 400
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to re-constitute the inhibitor for i.p. administration?

  • Answer:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

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