Torin 1

For research use only. Not for use in humans.

Catalog No.S2827

99 publications

Torin 1 Chemical Structure

Molecular Weight(MW): 607.62

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

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Selleck's Torin 1 has been cited by 99 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 Mlr5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTBwMECwN|A3KM7:TR?= MV3TRW5ITVJ?
OCUB-M NGrUbY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4Hu[WlEPTB;MD6wNFE6PCEQvF2= MlTJV2FPT0WU
DOHH-2 M1fQbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1HUemlEPTB;MD6wNFIzPSEQvF2= NYmyepN{W0GQR1XS
NOS-1 NXnWTZQ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTBwMECyOFIh|ryP MlTTV2FPT0WU
L-363 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTBwMECyPFQh|ryP NESyUXBUSU6JRWK=
RL95-2 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2r1T2lEPTB;MD6wNFMzKM7:TR?= MmHTV2FPT0WU
BL-70 M2LEWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HjemlEPTB;MD6wNFM{PyEQvF2= NWjPeW9KW0GQR1XS
TGBC1TKB NIPDXGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{f2N2lEPTB;MD6wNFM4QSEQvF2= MWfTRW5ITVJ?
697 NXLmR2FRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlrxTWM2OD1yLkCwOlM1KM7:TR?= MlfJV2FPT0WU
RPMI-8226 NYjRTodbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPZTWM2OD1yLkCwOlUyKM7:TR?= MlLmV2FPT0WU
BL-41 NFvMdldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rFS2lEPTB;MD6wNFY6PiEQvF2= NXW4b4VtW0GQR1XS
MRK-nu-1 MkXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTqXYdKSzVyPUCuNFA4ODdizszN M{\TT3NCVkeHUh?=
EW-1 M3K4bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlqyTWM2OD1yLkCwO|Q6KM7:TR?= Mn;JV2FPT0WU
EVSA-T NEW2N2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIi2fFdKSzVyPUCuNFA5QTJizszN M1n0OXNCVkeHUh?=
HUTU-80 Mk[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17XSGlEPTB;MD6wNFkzQSEQvF2= MXXTRW5ITVJ?
VA-ES-BJ Mn\wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;xRnpKSzVyPUCuNFA6QDRizszN M4rySXNCVkeHUh?=
GCIY NIH6PZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37qeGlEPTB;MD6wNVAxPyEQvF2= M4\RSXNCVkeHUh?=
ES6 M1;TUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojDTWM2OD1yLkCxNFg{KM7:TR?= MYrTRW5ITVJ?
NALM-6 MkHOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17TZ2lEPTB;MD6wNVExOiEQvF2= NH\XNnBUSU6JRWK=
LB996-RCC M17Dc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjQTWM2OD1yLkCxNVM4KM7:TR?= MkXiV2FPT0WU
EW-16 NWXJWokyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{L0RWlEPTB;MD6wNVE6OiEQvF2= NIj3flNUSU6JRWK=
KALS-1 NYrDUIdMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\Mc3VZUUN3ME2wMlAyOTl6IN88US=> MXzTRW5ITVJ?
OPM-2 M3\ldGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWO3NplVUUN3ME2wMlAyOjlizszN M{D0ZnNCVkeHUh?=
KU812 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnqyTWM2OD1yLkCxNlk3KM7:TR?= M2H4UHNCVkeHUh?=
A4-Fuk MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moq5TWM2OD1yLkCxN|E6KM7:TR?= NUnsSYdHW0GQR1XS
KGN MlPlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;Nb2N7UUN3ME2wMlAyOzV7IN88US=> MVnTRW5ITVJ?
KS-1 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTBwMEGzPVIh|ryP NXS0NGZmW0GQR1XS
no-11 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1Tpb2lEPTB;MD6wNVM6PSEQvF2= NFHGdmxUSU6JRWK=
PSN1 M{DDVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTBwMEG0NFkh|ryP MnvBV2FPT0WU
SK-LMS-1 M2[zd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTBwMEG0NVMh|ryP MmDqV2FPT0WU
LS-411N M2m0RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTBwMEG0N|kh|ryP M{\0bnNCVkeHUh?=
HT-144 NGLTVIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDONGtCUUN3ME2wMlAyPDVzIN88US=> NXXodIpLW0GQR1XS
ACN MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTBwMEG1JO69VQ>? MlzzV2FPT0WU
RKO MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLsTWM2OD1yLkCxOVM{KM7:TR?= MXTTRW5ITVJ?
CMK Mnr6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjHTWM2OD1yLkCxOVQ{KM7:TR?= M335TnNCVkeHUh?=
GB-1 NVH6NWY5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrWZm5yUUN3ME2wMlAyPTV|IN88US=> MU\TRW5ITVJ?
KE-37 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPMTWM2OD1yLkCxOVc{KM7:TR?= NUf0cY5sW0GQR1XS
no-10 M1nxWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojITWM2OD1yLkCxOVg1KM7:TR?= MYjTRW5ITVJ?
MOLT-16 M1j5Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlfTTWM2OD1yLkCxOlIzKM7:TR?= M3K1T3NCVkeHUh?=
HEL M1;1Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\IN|VOUUN3ME2wMlAyPjRizszN MXzTRW5ITVJ?
NCI-H1355 NYjKVW1vT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPoTWM2OD1yLkCxOlQ3KM7:TR?= MX\TRW5ITVJ?
IST-MEL1 M2LyWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTBwMEG3O|Qh|ryP MX7TRW5ITVJ?
MHH-PREB-1 MoDzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUntO|VvUUN3ME2wMlAyQDF6IN88US=> MlvEV2FPT0WU
ES8 NHiyfFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTBwMEG4OFQh|ryP Ml31V2FPT0WU
LC-2-ad M1:wOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTBwMEKxN|ch|ryP MXrTRW5ITVJ?
LAMA-84 NEPyZoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzKcJFDUUN3ME2wMlAzOTZ5IN88US=> MWTTRW5ITVJ?
BV-173 M13ZSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTBwMEKyNFMh|ryP Mm\SV2FPT0WU
SW872 NXfjXYdkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vLXmlEPTB;MD6wNlI1PyEQvF2= NVnKdGRoW0GQR1XS
SK-UT-1 NXXkfGFKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHX6VZhKSzVyPUCuNFIzPjFizszN Mkn2V2FPT0WU
OS-RC-2 MmniS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37BZWlEPTB;MD6wNlMzOyEQvF2= NIXHTXRUSU6JRWK=
NCI-H747 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWn5Z2J1UUN3ME2wMlAzOzNizszN NULxdGc{W0GQR1XS
MZ2-MEL MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LxdGlEPTB;MD6wNlM{PyEQvF2= M3zaeHNCVkeHUh?=
TE-11 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTBwMEKzO|Ih|ryP M{\PeXNCVkeHUh?=
KURAMOCHI Ml\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LNZ2lEPTB;MD6wNlQyPCEQvF2= MXjTRW5ITVJ?
HD-MY-Z MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTBwMEK0NlYh|ryP MXzTRW5ITVJ?
GI-1 Mmm5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwMEK0Olkh|ryP MVjTRW5ITVJ?
LB1047-RCC NHrmdYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDWeJhKSzVyPUCuNFI1QDhizszN M3LUb3NCVkeHUh?=
TE-12 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7uWnJsUUN3ME2wMlAzPTVizszN NWHHOZdEW0GQR1XS
MZ7-mel MkP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PiZ2lEPTB;MD6wNlYxQSEQvF2= NEH3e4RUSU6JRWK=
MMAC-SF MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILhfnhKSzVyPUCuNFI3OzNizszN NVTBR2pFW0GQR1XS
A101D NEPVTVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTBwMEK2OUDPxE1? MlvIV2FPT0WU
GR-ST NV;5cXAyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2juXWlEPTB;MD6wNlY2PSEQvF2= NUTaWGVPW0GQR1XS
MC116 MmjyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLCUGVKSzVyPUCuNFI3QCEQvF2= MnTOV2FPT0WU
Ramos-2G6-4C10 NE[yUXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvIXYlOUUN3ME2wMlAzPzVizszN M13rXnNCVkeHUh?=
ATN-1 NGHkW5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LvTmlEPTB;MD6wNlc5QSEQvF2= NUXUSYZtW0GQR1XS
MFH-ino MlO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1K3XGlEPTB;MD6wNlgyOiEQvF2= MmPJV2FPT0WU
DEL MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTBwMEK4NVUh|ryP Mn7oV2FPT0WU
OCI-AML2 Ml71S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU[wVVBCUUN3ME2wMlAzQDdzIN88US=> MkP1V2FPT0WU
LXF-289 MoLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPqdFZKSzVyPUCuNFI5PzJizszN NEHocWlUSU6JRWK=
IA-LM M17H[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH2yVZdKSzVyPUCuNFI6OTdizszN MWHTRW5ITVJ?
EoL-1-cell MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHGydnhKSzVyPUCuNFI6QTZizszN NYLyNm1PW0GQR1XS
OVCAR-4 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLkTWM2OD1yLkCzNFk1KM7:TR?= NWn5RphEW0GQR1XS
ES1 M2\1S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXJTWM2OD1yLkCzNFk4KM7:TR?= MVTTRW5ITVJ?
CESS MkXQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEn0RpJKSzVyPUCuNFMxQTlizszN MVzTRW5ITVJ?
GAK NIrSfJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3nTWM2OD1yLkCzNVg3KM7:TR?= M{jHVnNCVkeHUh?=
NTERA-S-cl-D1 NH;SPVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWridGxnUUN3ME2wMlA{OjJ4IN88US=> M4DmZ3NCVkeHUh?=
TE-10 M4XjbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwMEOzNVEh|ryP NIThZ|lUSU6JRWK=
CCRF-CEM MlfiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fMc2lEPTB;MD6wN|M4OSEQvF2= M2TqcnNCVkeHUh?=
NMC-G1 NHHoSnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTBwMEO0OFgh|ryP M4DNdHNCVkeHUh?=
HOP-62 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHjbXpKSzVyPUCuNFM2PTZizszN NIW5eFVUSU6JRWK=
SH-4 NVr6fVN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvrNmlKSzVyPUCuNFM2QDJizszN MmXEV2FPT0WU
NCI-H1648 NHHnNHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVW2XYFYUUN3ME2wMlA{PjJ{IN88US=> M2LBbXNCVkeHUh?=
EB-3 MoXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDjW|dKSzVyPUCuNFM3PDNizszN MYDTRW5ITVJ?
BB65-RCC NEKxU49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTBwMEO2PVEh|ryP MX;TRW5ITVJ?
BE-13 M{DXbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknMTWM2OD1yLkCzO|U6KM7:TR?= Mlr6V2FPT0WU
CP67-MEL MoPaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\OTIxKSzVyPUCuNFM4PTlizszN MYTTRW5ITVJ?
SW954 NFuxVIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljJTWM2OD1yLkCzO|kh|ryP MXzTRW5ITVJ?
TE-5 Mmr3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvJWYpKSzVyPUCuNFM5PTRizszN NYe1W4xDW0GQR1XS
HCE-4 NYXWcIY4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\tNVVLUUN3ME2wMlA{QDV3IN88US=> NXfJd5M4W0GQR1XS
LC-1F NVHsUnd6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnYUoRKSzVyPUCuNFM6QDlizszN M3LhdnNCVkeHUh?=
TK10 MkHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjIZZhNUUN3ME2wMlA1ODF7IN88US=> NYXwOG0xW0GQR1XS
SJSA-1 M4nUO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEjWW49KSzVyPUCuNFQyQDhizszN MYTTRW5ITVJ?
Daudi M3XOUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXBTWM2OD1yLkC0NlI2KM7:TR?= MXHTRW5ITVJ?
SW982 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvxTJp6UUN3ME2wMlA1OjR4IN88US=> NGe1V|FUSU6JRWK=
SF126 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEex[4lKSzVyPUCuNFQ{PjFizszN MlLCV2FPT0WU
TE-15 M3v5O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{DkXGlEPTB;MD6wOFQ{PSEQvF2= MlKxV2FPT0WU
COLO-684 M1rBOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHDT482UUN3ME2wMlA1PTZzIN88US=> M{DGT3NCVkeHUh?=
8-MG-BA NE\4RlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXpS4VKSzVyPUCuNFQ3OjlizszN NYDwbGhjW0GQR1XS
NB13 MljxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXG[ZZpUUN3ME2wMlA1Pzd4IN88US=> M{DXdHNCVkeHUh?=
HCC1599 MlPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTBwMES4O|kh|ryP MVnTRW5ITVJ?
SK-PN-DW MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVy2[5ZyUUN3ME2wMlA1QTR7IN88US=> M2nzfnNCVkeHUh?=
ALL-PO NHrPbFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrETWM2OD1yLkC0PVU3KM7:TR?= NIXjc45USU6JRWK=
NB6 MmrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFn2R|BKSzVyPUCuNFUzPzFizszN NGGwdINUSU6JRWK=
IST-SL2 M3zqOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXj3R2NHUUN3ME2wMlA2OzZ{IN88US=> MnzPV2FPT0WU
BB30-HNC MoSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{[xZWlEPTB;MD6wOVc{KM7:TR?= MUDTRW5ITVJ?
SW962 NXfMXZVsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTBwMEW3OlMh|ryP M{X4O3NCVkeHUh?=
TGBC24TKB MnnlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17KZWlEPTB;MD6wOVc6QSEQvF2= MlPVV2FPT0WU
KINGS-1 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHHTWM2OD1yLkC1PFA{KM7:TR?= NEf0UXhUSU6JRWK=
HC-1 NIL5XmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TYTGlEPTB;MD6wOVgzPyEQvF2= NXvRfIFkW0GQR1XS
RS4-11 M2e5RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFmxS2tKSzVyPUCuNFU6OzhizszN NYr4fGhnW0GQR1XS
LS-123 Mo\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnrqTWM2OD1yLkC1PVQzKM7:TR?= MVTTRW5ITVJ?
A3-KAW MmHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjneI5EUUN3ME2wMlA3OjJ3IN88US=> NVm4VFJMW0GQR1XS
SNB75 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmG2TWM2OD1yLkC2N|QyKM7:TR?= NYrrVJRKW0GQR1XS
SR NE\LUFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jqNmlEPTB;MD6wOlM4PCEQvF2= MX7TRW5ITVJ?
LOUCY MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;KTWlEPTB;MD6wOlM6PCEQvF2= MmnOV2FPT0WU
K5 M1LGdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrHSHY3UUN3ME2wMlA3PDB|IN88US=> MlXiV2FPT0WU
Becker NFGyO2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTBwME[0NkDPxE1? MY\TRW5ITVJ?
CTV-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVqzdmRNUUN3ME2wMlA3PTR6IN88US=> NIPYNWhUSU6JRWK=
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HCC1187 M1u5eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTBwNE[3Olgh|ryP NWXvWYtXW0GQR1XS
HCC2218 NHHnN|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7UbHFJUUN3ME2wMlQ4OjJ5IN88US=> M4rRNXNCVkeHUh?=
NCI-H187 M2PHTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrz[ldKSzVyPUCuOFc5PCEQvF2= M3XOdnNCVkeHUh?=
DB NYTudVduT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLtTWM2OD1yLkWwNFU2KM7:TR?= Mn\UV2FPT0WU
NCI-H69 NEHmSItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PXcmlEPTB;MD61NFI1PyEQvF2= NVrhNnlSW0GQR1XS
NCI-H1694 M1X3Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1W2O2lEPTB;MD61NFY6PyEQvF2= M1LtcXNCVkeHUh?=
CA46 M2Pibmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWe5RZhXUUN3ME2wMlUxQTF5IN88US=> NVLtVnZJW0GQR1XS
EHEB M4TV[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTBwNUGxPVMh|ryP MlPtV2FPT0WU
NCCIT NWq0V4RLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGW3ZmJKSzVyPUCuOVQ{OjFizszN NYnLWWVqW0GQR1XS
NCI-H720 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTBwNUS5OlIh|ryP M{[xXXNCVkeHUh?=
LU-134-A NHznUlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvN[IFKSzVyPUCuOVUzQTZizszN NGf3dW5USU6JRWK=
MC-CAR MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17zd2lEPTB;MD61OVQ{OSEQvF2= MUXTRW5ITVJ?
NEC8 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHfVSGpKSzVyPUCuOVYyPzJizszN M370RnNCVkeHUh?=
NCI-H524 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDPbmpKSzVyPUCuOVg4PDhizszN MV3TRW5ITVJ?
NCI-H1963 NUSyUHNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DDSmlEPTB;MD62NlU5KM7:TR?= NVT4W2lJW0GQR1XS
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NCI-H1522 MmDXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofhTWM2OD1yLk[0PFg{KM7:TR?= NUDHOYEyW0GQR1XS
NCI-H1155 NW\yPHM{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4rHRmlEPTB;MD62OVM2KM7:TR?= MmrLV2FPT0WU
D-263MG M3;wSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWT4coZUUUN3ME2wMlY3OTBzIN88US=> M2q3c3NCVkeHUh?=
TE-6 NICyUZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3GSYlKSzVyPUCuOlgyODRizszN NYnyRWlWW0GQR1XS
NCI-H1417 Mne4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPzTWM2OD1yLk[5O|EyKM7:TR?= NX3xO4ZuW0GQR1XS
NCI-H510A MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1m5WGlEPTB;MD63O|I{PiEQvF2= NUPjeW5{W0GQR1XS
KARPAS-45 NWPTOoFvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLNTXZuUUN3ME2wMlc5OjN3IN88US=> NEXNNVBUSU6JRWK=
IST-SL1 M{nvdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITKXohKSzVyPUCuPFU5OjVizszN Mor6V2FPT0WU
COLO-824 NFSxZnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEO4WlVKSzVyPUCuPFc5OzVizszN MUHTRW5ITVJ?
NCI-H526 MknlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HwfWlEPTB;MD65NFk2QCEQvF2= NGnRc4xUSU6JRWK=
NCI-H719 MmXUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jSemlEPTB;MD65OlY2PyEQvF2= NWjPT5hUW0GQR1XS
SF539 M2T4bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1f4SWlEPTB;MD65PFM6PiEQvF2= M{n2eHNCVkeHUh?=
NKM-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HSSGlEPTB;MD65PFY3OyEQvF2= MXTTRW5ITVJ?
A253 NVTQSmJET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4XhRmlEPTB;MD65PFg5PSEQvF2= MlzZV2FPT0WU
TE-441-T NXHsUoNiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEPtUFlKSzVyPUGuNFQ{PSEQvF2= MUHTRW5ITVJ?
COR-L88 M1zDdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGm2UlhKSzVyPUGuNVE{PTRizszN M2G1RnNCVkeHUh?=
CPC-N M3nMRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUf0TJRTUUN3ME2xMlE2QDh6IN88US=> M2PXTXNCVkeHUh?=
NCI-H2107 MkK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvrTWM2OD1zLkG3NlYyKM7:TR?= MWnTRW5ITVJ?
ECC4 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTFwMUi2PVMh|ryP M4XUe3NCVkeHUh?=
NCI-H2141 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmniTWM2OD1zLkG5Nlk6KM7:TR?= MVfTRW5ITVJ?
NCI-H1770 NIXITHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTFwM{K5Nlgh|ryP NInuU25USU6JRWK=
NCI-SNU-1 NVfmPZoyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHFTWM2OD1zLkOzNFQh|ryP M2nOSHNCVkeHUh?=
LU-165 Mkm3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4TFbmlEPTB;MT60OVQ3PyEQvF2= NHW0ToNUSU6JRWK=
Raji NX\Qc2M1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTFwNEeyOVkh|ryP NI\2Z|lUSU6JRWK=
EW-24 M4CxXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYe1Z5R1UUN3ME2xMlU1PjF7IN88US=> NVj6ZnhqW0GQR1XS
KP-N-RT-BM-1 M1[1fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYm1S4lSUUN3ME2xMlU3PTVzIN88US=> MWPTRW5ITVJ?
SK-MEL-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXz[lBpUUN3ME2xMlY1QDd5IN88US=> NYDreYhyW0GQR1XS
SCLC-21H M4LncWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTFwNk[2OVEh|ryP NEfZRo1USU6JRWK=
SIMA MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mom1TWM2OD1zLk[4NlM{KM7:TR?= NHHHd3FUSU6JRWK=
NCI-H1304 NYnaXYVIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nQR2lEPTB;MT63NFc3PSEQvF2= Ml3RV2FPT0WU
REH NXvCc5FoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm[4TWM2OD1zLkexNFc5KM7:TR?= NEPzT2dUSU6JRWK=
KP-N-YS NILieIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml30TWM2OD1zLkmzOVUyKM7:TR?= NXy4[IkzW0GQR1XS
DMS-153 NGDmUndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7qTY9KSzVyPUGuPVc2QSEQvF2= NEnGcXNUSU6JRWK=
NB10 M2LLSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTMdI15UUN3ME2yMlIxQDd6IN88US=> NWrZTWw2W0GQR1XS
NCI-H345 Mm\1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoSwTWM2OD1{Lk[xPVA2KM7:TR?= MljoV2FPT0WU
SK-N-FI MnjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjuWoVKSzVyPUKuO|U{QDFizszN MXnTRW5ITVJ?
NCI-H1092 NETMV3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVT3bYZ3UUN3ME2yMlk2Ozl2IN88US=> NV64ZXZIW0GQR1XS
KM-H2 M3XRc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzKO48{UUN3ME2zMlY2Ozl4IN88US=> Ml3TV2FPT0WU
KG-1 NV\pV4ZJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnn1TWM2OD1|LkexOlg5KM7:TR?= Ml\0V2FPT0WU
D-283MED NUjFN4dnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTNwN{W4O{DPxE1? NIjUbIdUSU6JRWK=
LU-65 NYezb3JGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPtSFRKSzVyPUSuNVE1QDVizszN NYLCXnd6W0GQR1XS
MSTO-211H NXPsXmkyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTRwMUi4PVgh|ryP M3\ObXNCVkeHUh?=
NCI-H2081 NVL0[JhVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3QcnpqUUN3ME20MlI6OTd2IN88US=> NUjLVpF4W0GQR1XS
D-247MG NFLWV|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;ocJFWUUN3ME20MlQ{OTlzIN88US=> MkDEV2FPT0WU
P31-FUJ MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PFOGlEPTB;ND60OVAyQSEQvF2= NF;kcXRUSU6JRWK=
DMS-79 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLI[HRKSzVyPUSuPVMyQDNizszN M{HhW3NCVkeHUh?=
KASUMI-1 NIjqeWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLuTWM2OD12Lkm3OVA6KM7:TR?= MV3TRW5ITVJ?
JVM-3 M3:5VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTVwMkWwNVQh|ryP MojKV2FPT0WU
LAN-6 NIPrRppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFm0cnhKSzVyPUWuOVE4OzZizszN NE\0eIhUSU6JRWK=
DJM-1 M2W4N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPHe3BCUUN3ME21MlU3ODJ7IN88US=> MWnTRW5ITVJ?
JVM-2 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTVwNkewPVIh|ryP MWHTRW5ITVJ?
U-87-MG NWG3dYlVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4q2WmlEPTB;NT64PVk3PCEQvF2= M3[3NHNCVkeHUh?=
SHP-77 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3ocYxKSzVyPUWuPVQ6OjhizszN NEDncIVUSU6JRWK=
RPMI-8866 M2\tN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH3OTodKSzVyPU[uNlcxOTNizszN NX7afop7W0GQR1XS
ES7 Mn[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoC0TWM2OD14LkO5PVI1KM7:TR?= M4fieHNCVkeHUh?=
WSU-NHL Ml;SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTZwNUG2NFkh|ryP NXPCcHI5W0GQR1XS
CAS-1 NH3KeVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rwWGlEPTB;Nz6wPVc1QSEQvF2= M1rzTHNCVkeHUh?=
MHH-NB-11 NX33e3VbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HTdmlEPTB;OD6yN|E2OiEQvF2= MXvTRW5ITVJ?
ES5 M3\uZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTlwNUKyPFYh|ryP Mk\CV2FPT0WU
HDLM-2 NGrGXVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLUdppKSzVyPUmuPVU6ODJizszN NXrONVN6W0GQR1XS
NCI-H2171 M1v6W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTFyLkW5Nlch|ryP MkPhV2FPT0WU
LNCaP-Clone-FGC NW\IR493T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTFzLkO3NVgh|ryP NGrGN4VUSU6JRWK=
NCI-H1395 MoHoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHfMWW9KSzVyPUGyMlI3QTlizszN MnfMV2FPT0WU
NCI-H1436 NE\BZmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTYXYF1UUN3ME2xN{4{OTl3IN88US=> NYTlO5E1W0GQR1XS
RCC10RGB M3HZO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTF|LkOyNlEh|ryP NH\2[IJUSU6JRWK=
SW684 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF[wfYVKSzVyPUGzMlk4QTdizszN NWfleFM3W0GQR1XS
NCI-H1650 M2XIe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV31S|hnUUN3ME2xOU4xPDR5IN88US=> MonJV2FPT0WU
EW-13 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjDcohHUUN3ME2xOU4zODJzIN88US=> MX3TRW5ITVJ?
EC-GI-10 M4P1PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTF3Lki2OFQh|ryP MVjTRW5ITVJ?
CHP-126 M{\QSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rzfGlEPTB;MUeuNVkyQCEQvF2= M4T3W3NCVkeHUh?=
NCI-H226 MnPCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7UTWM2OD1zOD6wOFA{KM7:TR?= MWLTRW5ITVJ?
NCI-H1838 Mmf5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnuRnp{UUN3ME2yNE4yQDR6IN88US=> NVr2dZVOW0GQR1XS
EW-11 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHWzSnNKSzVyPUK0MlI4OThizszN MWLTRW5ITVJ?
EB2 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHpTWM2OD1|MT61N|E5KM7:TR?= MU\TRW5ITVJ?
D-502MG M3;2NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLpTWM2OD12Mz65NlI6KM7:TR?= MUjTRW5ITVJ?
U-698-M M3HsNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7ISZNKSzVyPUS4Mlg3PzFizszN NHGzXpFUSU6JRWK=
ES3 NFfoSYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzDNWF{UUN3ME20PU4{QDl|IN88US=> NWToOJZQW0GQR1XS

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol

Kinase Assay:[1]
- Collapse

mTORC1 and mTORC2 in Vitro Kinase Assays:

To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research:[1]
- Collapse
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: U87MG xenograft model
  • Formulation: Torin1 powder is first dissolved at 25 mg/mL in 100% N-methyl-2-pyrrolidone and subsequently diluted 1:4 with sterile 50% PEG400 to a final concentration of 5 mg/mL
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
20% NMP+50% PEG 400
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage powder
in solvent
Synonyms N/A
Smiles CCC(=O)N1CCN(CC1)C2=CC=C(C=C2C(F)(F)F)N3C(=O)C=CC4=C3C5=CC(=CC=C5N=C4)C6=CC7=CC=CC=C7N=C6

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    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to re-constitute the inhibitor for i.p. administration?

  • Answer:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID