Torin 1

For research use only.

Catalog No.S2827

115 publications

Torin 1 Chemical Structure

Molecular Weight(MW): 607.62

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

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Selleck's Torin 1 has been cited by 115 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 NXfwPI1qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmriTWM2OD1yLkCwNFMxPiEQvF2= MnLzV2FPT0WU
OCUB-M NETJbYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DxUGlEPTB;MD6wNFE6PCEQvF2= NWLUeIJPW0GQR1XS
DOHH-2 NWnvXXI{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3Xuc2lEPTB;MD6wNFIzPSEQvF2= NHvodW9USU6JRWK=
NOS-1 M1qzUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDSTWM2OD1yLkCwNlQzKM7:TR?= M1\hWXNCVkeHUh?=
L-363 NWnD[4N4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTTTWM2OD1yLkCwNlg1KM7:TR?= MULTRW5ITVJ?
RL95-2 NILlcItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;McGlEPTB;MD6wNFMzKM7:TR?= MWTTRW5ITVJ?
BL-70 Mn\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTBwMECzN|ch|ryP NYfzbWh6W0GQR1XS
TGBC1TKB MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfUUnpKSzVyPUCuNFA{PzlizszN MlvtV2FPT0WU
697 NEi2O3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorNTWM2OD1yLkCwOlM1KM7:TR?= NUO2RmhDW0GQR1XS
RPMI-8226 NFHQUppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2i2RmlEPTB;MD6wNFY2OSEQvF2= MYfTRW5ITVJ?
BL-41 MnrmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{[zbmlEPTB;MD6wNFY6PiEQvF2= NFzifJNUSU6JRWK=
MRK-nu-1 NGS0TYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HsNWlEPTB;MD6wNFcxPyEQvF2= MYjTRW5ITVJ?
EW-1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjpdm1KSzVyPUCuNFA4PDlizszN MlHSV2FPT0WU
EVSA-T NI\SOpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoWwTWM2OD1yLkCwPFkzKM7:TR?= NYXtW|dKW0GQR1XS
HUTU-80 NIPmb|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEP1N|JKSzVyPUCuNFA6OjlizszN M4DkVnNCVkeHUh?=
VA-ES-BJ NIrvbVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1H6VWlEPTB;MD6wNFk5PCEQvF2= MXnTRW5ITVJ?
GCIY MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTBwMEGwNFch|ryP M4rzfHNCVkeHUh?=
ES6 Mnj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkT2TWM2OD1yLkCxNFg{KM7:TR?= NVvEV45rW0GQR1XS
NALM-6 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHCTWM2OD1yLkCxNVAzKM7:TR?= NXrhSWFlW0GQR1XS
LB996-RCC MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH3RZYJKSzVyPUCuNFEyOzdizszN MUPTRW5ITVJ?
EW-16 NX;mT4p3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTBwMEGxPVIh|ryP NYfhVWViW0GQR1XS
KALS-1 MoXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTBwMEGxPVgh|ryP MXzTRW5ITVJ?
OPM-2 M33LUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrYTWM2OD1yLkCxNlkh|ryP NHfDOolUSU6JRWK=
KU812 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHHbXdKSzVyPUCuNFEzQTZizszN M2DsW3NCVkeHUh?=
A4-Fuk MnvsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzqXoRkUUN3ME2wMlAyOzF7IN88US=> NELPXWNUSU6JRWK=
KGN MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmflTWM2OD1yLkCxN|U6KM7:TR?= MVzTRW5ITVJ?
KS-1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTBwMEGzPVIh|ryP MoPMV2FPT0WU
no-11 M1rLZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLEdWxQUUN3ME2wMlAyOzl3IN88US=> MV\TRW5ITVJ?
PSN1 Mne0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTBwMEG0NFkh|ryP NE[2c2pUSU6JRWK=
SK-LMS-1 Ml[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEf2SXBKSzVyPUCuNFE1OTNizszN M4\IN3NCVkeHUh?=
LS-411N NEXnb5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTvO2dlUUN3ME2wMlAyPDN7IN88US=> MnHQV2FPT0WU
HT-144 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LObmlEPTB;MD6wNVQ2OSEQvF2= MnXOV2FPT0WU
ACN NHPiXoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLPTWM2OD1yLkCxOUDPxE1? M4fxRXNCVkeHUh?=
RKO NFzRO5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\QTWM2OD1yLkCxOVM{KM7:TR?= MWTTRW5ITVJ?
CMK NEH5UGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPZeVdKSzVyPUCuNFE2PDNizszN MYfTRW5ITVJ?
GB-1 NIfXS4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2foV2lEPTB;MD6wNVU2OyEQvF2= MkPFV2FPT0WU
KE-37 MlzNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mki2TWM2OD1yLkCxOVc{KM7:TR?= NYP5TZp6W0GQR1XS
no-10 M3vxTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHSSGdKSzVyPUCuNFE2QDRizszN NUXrSXFlW0GQR1XS
MOLT-16 NV\lcmJ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYKxd3RWUUN3ME2wMlAyPjJ{IN88US=> NFXscYpUSU6JRWK=
HEL MmXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXuTWM2OD1yLkCxOlQh|ryP MXXTRW5ITVJ?
NCI-H1355 M2D4[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TDOWlEPTB;MD6wNVY1PiEQvF2= M3H4R3NCVkeHUh?=
IST-MEL1 M3PZ[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTBwMEG3O|Qh|ryP MUfTRW5ITVJ?
MHH-PREB-1 MlTDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjRTWM2OD1yLkCxPFE5KM7:TR?= NH;GOJJUSU6JRWK=
ES8 M1XaNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTBwMEG4OFQh|ryP MoHIV2FPT0WU
LC-2-ad M1q3Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIS0UGFKSzVyPUCuNFIyOzdizszN MmLGV2FPT0WU
LAMA-84 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXtVGxKSzVyPUCuNFIyPjdizszN M{LNNnNCVkeHUh?=
BV-173 NYThXm1yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGC5T3ZKSzVyPUCuNFIzODNizszN MYHTRW5ITVJ?
SW872 MmnHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoD5TWM2OD1yLkCyNlQ4KM7:TR?= NET3TGlUSU6JRWK=
SK-UT-1 MoD6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkCxTWM2OD1yLkCyNlYyKM7:TR?= M2\FRnNCVkeHUh?=
OS-RC-2 NXvhRVBtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrlVFhKSzVyPUCuNFI{OjNizszN M{fwfXNCVkeHUh?=
NCI-H747 NFez[|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn25TWM2OD1yLkCyN|Mh|ryP MnrhV2FPT0WU
MZ2-MEL M4rhWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHSbpJKSzVyPUCuNFI{OzdizszN NH3aUVRUSU6JRWK=
TE-11 NXPMNolNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{f6SmlEPTB;MD6wNlM4OiEQvF2= MWfTRW5ITVJ?
KURAMOCHI MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLIS|M6UUN3ME2wMlAzPDF2IN88US=> NWL0SnZGW0GQR1XS
HD-MY-Z MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTBwMEK0NlYh|ryP NWLmOlJIW0GQR1XS
GI-1 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3njSGlEPTB;MD6wNlQ3QSEQvF2= NGjhWm5USU6JRWK=
LB1047-RCC M4\xdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTBwMEK0PFgh|ryP Mnf4V2FPT0WU
TE-12 NUG0W49pT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlK2TWM2OD1yLkCyOVUh|ryP MmLvV2FPT0WU
MZ7-mel NWmybZdbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLIRo9FUUN3ME2wMlAzPjB7IN88US=> NXm3fZRPW0GQR1XS
MMAC-SF M1vwbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTBwMEK2N|Mh|ryP MnXWV2FPT0WU
A101D MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLPTWM2OD1yLkCyOlUh|ryP MmqzV2FPT0WU
GR-ST MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTBwMEK2OVUh|ryP NY\UWnVJW0GQR1XS
MC116 MlTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXO1VYI4UUN3ME2wMlAzPjhizszN M3vpUnNCVkeHUh?=
Ramos-2G6-4C10 M1jjXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkezTWM2OD1yLkCyO|Uh|ryP NVnDXW5wW0GQR1XS
ATN-1 NGLMZZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{Lq[WlEPTB;MD6wNlc5QSEQvF2= NYfTfVJIW0GQR1XS
MFH-ino NXrRU4g1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFuxT3VKSzVyPUCuNFI5OTJizszN NEKzVFJUSU6JRWK=
DEL MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LSeWlEPTB;MD6wNlgyPSEQvF2= NIO1b|dUSU6JRWK=
OCI-AML2 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvTR|hKSzVyPUCuNFI5PzFizszN MUnTRW5ITVJ?
LXF-289 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTBwMEK4O|Ih|ryP NHTpdW5USU6JRWK=
IA-LM Ml\CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHoUmFKSzVyPUCuNFI6OTdizszN MlXkV2FPT0WU
EoL-1-cell M4TDc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\iO2lEPTB;MD6wNlk6PiEQvF2= NGDHTIpUSU6JRWK=
OVCAR-4 M2HkRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\PbpRJUUN3ME2wMlA{ODl2IN88US=> MVjTRW5ITVJ?
ES1 NGi0O|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\LTWM2OD1yLkCzNFk4KM7:TR?= M4HQVnNCVkeHUh?=
CESS NGPBOpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV3xem5EUUN3ME2wMlA{ODl7IN88US=> M175NHNCVkeHUh?=
GAK NY[zOpVlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfCVYNKSzVyPUCuNFMyQDZizszN NWPaXWFXW0GQR1XS
NTERA-S-cl-D1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDKOWY{UUN3ME2wMlA{OjJ4IN88US=> M4njZnNCVkeHUh?=
TE-10 M{DpNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnpNIt5UUN3ME2wMlA{OzFzIN88US=> M4TsenNCVkeHUh?=
CCRF-CEM NF3PNZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHaTWM2OD1yLkCzN|cyKM7:TR?= NInybYRUSU6JRWK=
NMC-G1 NX;4V4ZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3CTWM2OD1yLkCzOFQ5KM7:TR?= MX7TRW5ITVJ?
HOP-62 MnfyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\3O4loUUN3ME2wMlA{PTV4IN88US=> MX\TRW5ITVJ?
SH-4 NXTzUI9zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI[xephKSzVyPUCuNFM2QDJizszN NU\ONZAzW0GQR1XS
NCI-H1648 NWDwd49IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLJTWM2OD1yLkCzOlIzKM7:TR?= MmW4V2FPT0WU
EB-3 NFHvcY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;YO5NKSzVyPUCuNFM3PDNizszN NVHJTlhYW0GQR1XS
BB65-RCC NXvVN|h6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;YVI9sUUN3ME2wMlA{PjlzIN88US=> MYDTRW5ITVJ?
BE-13 NFjmW21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHITWM2OD1yLkCzO|U6KM7:TR?= M2\GdXNCVkeHUh?=
CP67-MEL MoXNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jXbmlEPTB;MD6wN|c2QSEQvF2= MlrBV2FPT0WU
SW954 Mm\oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPpTWM2OD1yLkCzO|kh|ryP NIjPUotUSU6JRWK=
TE-5 NEjqUm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DhOGlEPTB;MD6wN|g2PCEQvF2= M4D5dHNCVkeHUh?=
HCE-4 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7zTWM2OD1yLkCzPFU2KM7:TR?= MVTTRW5ITVJ?
LC-1F NH:yN|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPmNWpKSzVyPUCuNFM6QDlizszN NUPscIp3W0GQR1XS
TK10 NIHGS2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEG5UoFKSzVyPUCuNFQxOTlizszN M{\pTnNCVkeHUh?=
SJSA-1 NYXT[no4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITj[VlKSzVyPUCuNFQyQDhizszN MXrTRW5ITVJ?
Daudi MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTBwMESyNlUh|ryP MXPTRW5ITVJ?
SW982 M4PlUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTiSGRJUUN3ME2wMlA1OjR4IN88US=> MUPTRW5ITVJ?
SF126 MkjUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rHVWlEPTB;MD6wOFM3OSEQvF2= M{fGenNCVkeHUh?=
TE-15 M1mxVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{T6WGlEPTB;MD6wOFQ{PSEQvF2= MUXTRW5ITVJ?
COLO-684 M4Lxfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX:xSllbUUN3ME2wMlA1PTZzIN88US=> NXHzcGpnW0GQR1XS
8-MG-BA MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPUTWM2OD1yLkC0OlI6KM7:TR?= M1vyWHNCVkeHUh?=
NB13 M4\Be2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\T[2RKSzVyPUCuNFQ4PzZizszN NHH0OIxUSU6JRWK=
HCC1599 NHK1dmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTBwMES4O|kh|ryP NYXOb5FkW0GQR1XS
SK-PN-DW MoXSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mne2TWM2OD1yLkC0PVQ6KM7:TR?= NWHGWo1jW0GQR1XS
ALL-PO MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\MVYlKSzVyPUCuNFQ6PTZizszN MXLTRW5ITVJ?
NB6 NYX3fZV5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXy5R3RTUUN3ME2wMlA2OjdzIN88US=> MkLhV2FPT0WU
IST-SL2 NITHd4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHj5Z3pKSzVyPUCuNFU{PjJizszN MUDTRW5ITVJ?
BB30-HNC M2jrRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYT6XoJGUUN3ME2wMlA2PzNizszN MVPTRW5ITVJ?
SW962 Mn32S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwMEW3OlMh|ryP NFexe|lUSU6JRWK=
TGBC24TKB NVnSSFFlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzxdnVWUUN3ME2wMlA2Pzl7IN88US=> MmS3V2FPT0WU
KINGS-1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\pVZZKSzVyPUCuNFU5ODNizszN MV7TRW5ITVJ?
HC-1 NHPNfHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HwUWlEPTB;MD6wOVgzPyEQvF2= Mm\oV2FPT0WU
RS4-11 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PaPGlEPTB;MD6wOVk{QCEQvF2= NV;RO481W0GQR1XS
LS-123 MkLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPTPWNKSzVyPUCuNFU6PDJizszN NWXjdW9mW0GQR1XS
A3-KAW M1nWNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XKSWlEPTB;MD6wOlIzPSEQvF2= NYXTS5RMW0GQR1XS
SNB75 NIHueVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFP0fmNKSzVyPUCuNFY{PDFizszN MYrTRW5ITVJ?
SR NHPVUJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTBwME[zO|Qh|ryP Ml7kV2FPT0WU
LOUCY MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrlTWM2OD1yLkC2N|k1KM7:TR?= MoG1V2FPT0WU
K5 M37xSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTBwME[0NFMh|ryP MXLTRW5ITVJ?
Becker MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;2R2lEPTB;MD6wOlQzKM7:TR?= MV\TRW5ITVJ?
CTV-1 MnG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\tcoNyUUN3ME2wMlA3PTR6IN88US=> Mkf4V2FPT0WU
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NCI-H187 NXOwSYM3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTNcIdVUUN3ME2wMlQ4QDRizszN M{LLOHNCVkeHUh?=
DB NX\3bJVOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DuOmlEPTB;MD61NFA2PSEQvF2= MkTTV2FPT0WU
NCI-H69 M2rF[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrGbZhKSzVyPUCuOVAzPDdizszN NFvGOZdUSU6JRWK=
NCI-H1694 MlPIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfsTWM2OD1yLkWwOlk4KM7:TR?= MoDLV2FPT0WU
CA46 NUC1VYJYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTBwNUC5NVch|ryP NEH0eXlUSU6JRWK=
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MC-CAR MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHIU41sUUN3ME2wMlU2PDNzIN88US=> Mn25V2FPT0WU
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NCI-H1963 M1fMUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vQbGlEPTB;MD62NlU5KM7:TR?= MlTWV2FPT0WU
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NCI-H1522 MmO1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3ToWmlEPTB;MD62OFg5OyEQvF2= NHfQ[nhUSU6JRWK=
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NCI-H510A MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPCTWM2OD1yLke3NlM3KM7:TR?= NXO1fopjW0GQR1XS
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NCI-H719 Mm\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjiPXJCUUN3ME2wMlk3PjV5IN88US=> MYDTRW5ITVJ?
SF539 NXLES4NqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PUTWlEPTB;MD65PFM6PiEQvF2= M4C1cnNCVkeHUh?=
NKM-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTBwOUi2OlMh|ryP MXHTRW5ITVJ?
A253 MoTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFL0eGxKSzVyPUCuPVg5QDVizszN NH7qcHhUSU6JRWK=
TE-441-T NXnHN3NpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTFwMESzOUDPxE1? MVLTRW5ITVJ?
COR-L88 MlK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWKxZm14UUN3ME2xMlEyOzV2IN88US=> MWnTRW5ITVJ?
CPC-N Mlv1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWT1ZVU{UUN3ME2xMlE2QDh6IN88US=> NGjwb2VUSU6JRWK=
NCI-H2107 NFTGfGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rNWGlEPTB;MT6xO|I3OSEQvF2= MkixV2FPT0WU
ECC4 M3nIUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjiTWM2OD1zLkG4Olk{KM7:TR?= MYPTRW5ITVJ?
NCI-H2141 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zPWWlEPTB;MT6xPVI6QSEQvF2= NX7LTZFSW0GQR1XS
NCI-H1770 M{PUUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LFU2lEPTB;MT6zNlkzQCEQvF2= Mo\EV2FPT0WU
NCI-SNU-1 MkfZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;lSHNKSzVyPUGuN|MxPCEQvF2= MVzTRW5ITVJ?
LU-165 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4WzR2lEPTB;MT60OVQ3PyEQvF2= M1T6cnNCVkeHUh?=
Raji M1\VUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHqWGFKSzVyPUGuOFczPTlizszN MX7TRW5ITVJ?
EW-24 M1j5eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnmTWM2OD1zLkW0OlE6KM7:TR?= NWPROWc4W0GQR1XS
KP-N-RT-BM-1 NVzRWHdIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjYXZNKSzVyPUGuOVY2PTFizszN NYTMc41RW0GQR1XS
SK-MEL-1 M4fsTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTFwNkS4O|ch|ryP M4\keXNCVkeHUh?=
SCLC-21H MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLiblJFUUN3ME2xMlY3PjVzIN88US=> NVK5OJhkW0GQR1XS
SIMA Ml7QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTFwNkiyN|Mh|ryP MX3TRW5ITVJ?
NCI-H1304 NGj1c2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnjTWM2OD1zLkewO|Y2KM7:TR?= MmLHV2FPT0WU
REH NHnKOJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTFwN{GwO|gh|ryP M4Toe3NCVkeHUh?=
KP-N-YS M1HvOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;QW2lEPTB;MT65N|U2OSEQvF2= NF30WHhUSU6JRWK=
DMS-153 NYDlcpVCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHYTWM2OD1zLkm3OVkh|ryP MVLTRW5ITVJ?
NB10 NGLWUpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTJwMkC4O|gh|ryP NH7JNIdUSU6JRWK=
NCI-H345 M3TqWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTJwNkG5NFUh|ryP MWTTRW5ITVJ?
SK-N-FI MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrHe3lvUUN3ME2yMlc2OzhzIN88US=> M2DwNXNCVkeHUh?=
NCI-H1092 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{L3WGlEPTB;Mj65OVM6PCEQvF2= NGK3RYZUSU6JRWK=
KM-H2 MoXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIm0TopKSzVyPUOuOlU{QTZizszN NHfvc3RUSU6JRWK=
KG-1 MnHxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfrTWM2OD1|LkexOlg5KM7:TR?= MojHV2FPT0WU
D-283MED MmXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3QPFJKSzVyPUOuO|U5PyEQvF2= NEXhcFZUSU6JRWK=
LU-65 NVrEPYtxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTrTWM2OD12LkGxOFg2KM7:TR?= NVjEVGIxW0GQR1XS
MSTO-211H MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLMTWM2OD12LkG4PFk5KM7:TR?= MkDjV2FPT0WU
NCI-H2081 NW[3[oxiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGftUHJKSzVyPUSuNlkyPzRizszN M1joOXNCVkeHUh?=
D-247MG M{jwXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDUfnpKSzVyPUSuOFMyQTFizszN NWTi[pI{W0GQR1XS
P31-FUJ NFXnc|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTpR|EyUUN3ME20MlQ2ODF7IN88US=> NXXGS5dMW0GQR1XS
DMS-79 NFjxSW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2D2SWlEPTB;ND65N|E5OyEQvF2= NX7Wc41RW0GQR1XS
KASUMI-1 NHLpZVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvDOopKSzVyPUSuPVc2ODlizszN NEWyZ|ZUSU6JRWK=
JVM-3 M4nMc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTVwMkWwNVQh|ryP NVjwenBJW0GQR1XS
LAN-6 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTVwNUG3N|Yh|ryP NEfO[IRUSU6JRWK=
DJM-1 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHy5eI5KSzVyPUWuOVYxOjlizszN NEP6[4FUSU6JRWK=
JVM-2 NWG2ZZJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTVwNkewPVIh|ryP MnvNV2FPT0WU
U-87-MG NXf6dnJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYL3b5IxUUN3ME21Mlg6QTZ2IN88US=> MWTTRW5ITVJ?
SHP-77 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3sNnRKSzVyPUWuPVQ6OjhizszN NYHm[W1nW0GQR1XS
RPMI-8866 NX;0Z2RkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnveo5nUUN3ME22MlI4ODF|IN88US=> M1rvUXNCVkeHUh?=
ES7 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2fuWmlEPTB;Nj6zPVkzPCEQvF2= MlzkV2FPT0WU
WSU-NHL M1;vNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzHe3VKSzVyPU[uOVE3ODlizszN MUjTRW5ITVJ?
CAS-1 MmXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlOzTWM2OD15LkC5O|Q6KM7:TR?= MnHIV2FPT0WU
MHH-NB-11 NHe5WIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;pTWM2OD16LkKzNVUzKM7:TR?= M3W2N3NCVkeHUh?=
ES5 NWfWWolsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTlwNUKyPFYh|ryP NVj3UYRtW0GQR1XS
HDLM-2 MkXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHhT|l6UUN3ME25Mlk2QTB{IN88US=> MUHTRW5ITVJ?
NCI-H2171 NXq3fIRFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTFyLkW5Nlch|ryP NX\3emhQW0GQR1XS
LNCaP-Clone-FGC NE\HZoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\MTWM2OD1zMT6zO|E5KM7:TR?= M4qxWHNCVkeHUh?=
NCI-H1395 NYPhb4xUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LmcmlEPTB;MUKuNlY6QSEQvF2= M3qxdXNCVkeHUh?=
NCI-H1436 M4\CeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTF|LkOxPVUh|ryP MoPMV2FPT0WU
RCC10RGB NXvhd5A4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7rTWM2OD1zMz6zNlIyKM7:TR?= NUL6ZZpbW0GQR1XS
SW684 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\ETWM2OD1zMz65O|k4KM7:TR?= M2PPb3NCVkeHUh?=
NCI-H1650 NHTqOVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmeyTWM2OD1zNT6wOFQ4KM7:TR?= MXPTRW5ITVJ?
EW-13 MmLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTF3LkKwNlEh|ryP M1nPUHNCVkeHUh?=
EC-GI-10 Moj3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYi5SnVsUUN3ME2xOU45PjR2IN88US=> NWjZR|RwW0GQR1XS
CHP-126 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITVdo9KSzVyPUG3MlE6OThizszN M2LlUXNCVkeHUh?=
NCI-H226 Mm\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nLcGlEPTB;MUiuNFQxOyEQvF2= MmXkV2FPT0WU
NCI-H1838 MkPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrYTWM2OD1{MD6xPFQ5KM7:TR?= NF71XGtUSU6JRWK=
EW-11 M3HTWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nWWmlEPTB;MkSuNlcyQCEQvF2= MULTRW5ITVJ?
EB2 NXP6UoNJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnpSppHUUN3ME2zNU42OzF6IN88US=> M33GTHNCVkeHUh?=
D-502MG MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPj[GRKSzVyPUSzMlkzOjlizszN M1;w[nNCVkeHUh?=
U-698-M M176Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLme5hJUUN3ME20PE45PjdzIN88US=> NEmyN3pUSU6JRWK=
ES3 MknGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTCWZlKSzVyPUS5MlM5QTNizszN Moj1V2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol

Kinase Assay:[1]
- Collapse

mTORC1 and mTORC2 in Vitro Kinase Assays:

To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research:[1]
- Collapse
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: U87MG xenograft model
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
20% NMP+50% PEG 400
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage powder
in solvent
Synonyms N/A
Smiles CCC(=O)N1CCN(CC1)C2=CC=C(C=C2C(F)(F)F)N3C(=O)C=CC4=C3C5=CC(=CC=C5N=C4)C6=CC7=CC=CC=C7N=C6

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O
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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to re-constitute the inhibitor for i.p. administration?

  • Answer:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID