AZD8055

For research use only. Not for use in humans.

Catalog No.S1555

119 publications

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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Selleck's AZD8055 has been cited by 119 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\4R2tKSzVyPUCuNFI{OzFizszN Mk\mV2FPT0WU
NCI-H720 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTBwMEK5N|gh|ryP M17ZW3NCVkeHUh?=
KARPAS-45 M1fFbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfqRYRNUUN3ME2wMlA{OzdzIN88US=> NWS5NXM{W0GQR1XS
EW-3 M4PJUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTBwMESwOVYh|ryP NHH4[FZUSU6JRWK=
SBC-1 NGrhZoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzofHJ1UUN3ME2wMlA1PThzIN88US=> M3\jVHNCVkeHUh?=
NCI-H209 NVrkZXpYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnKcI9KSzVyPUCuNFU1PTNizszN NF3pZ|lUSU6JRWK=
EoL-1-cell NVyyNnE{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnT0TWM2OD1yLkC2OVY6KM7:TR?= NIjiTW5USU6JRWK=
H9 MlXNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1Hte2lEPTB;MD6wOlY6OiEQvF2= M4XGPHNCVkeHUh?=
AN3-CA MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnBTWM2OD1yLkC3PVUyKM7:TR?= Mn;HV2FPT0WU
HH MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTBwMEiwNFkh|ryP MkO0V2FPT0WU
KU812 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLWVo5CUUN3ME2wMlA5ODR|IN88US=> NYOxdVBSW0GQR1XS
DU-4475 Mn;6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4XMUmlEPTB;MD6wPFA1PyEQvF2= Ml24V2FPT0WU
CTV-1 NEizSZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfITWM2OD1yLkC4OlQyKM7:TR?= MkLGV2FPT0WU
BV-173 M2Lh[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTBwMEmwOlEh|ryP MWPTRW5ITVJ?
ES5 MkDCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HnUWlEPTB;MD6wPVM2KM7:TR?= M3\MRXNCVkeHUh?=
G-402 NIf2bHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLKTYhOUUN3ME2wMlA6QThizszN MoHuV2FPT0WU
MV-4-11 NWj2bYxNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETCN4pKSzVyPUCuNVA{PDdizszN M3ThUHNCVkeHUh?=
NB69 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTBwMUO1JO69VQ>? MV3TRW5ITVJ?
EGI-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfxUIZJUUN3ME2wMlE{PTNzIN88US=> Mn3NV2FPT0WU
EW-18 M4n6U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILXZlJKSzVyPUCuNVQxPzNizszN NF7SUolUSU6JRWK=
ME-180 NH7Qc5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDmZmRKSzVyPUCuNVQ3QDFizszN NYjyNWFuW0GQR1XS
D-283MED NXTMc|U3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmWyTWM2OD1yLkG0O|I{KM7:TR?= Ml\PV2FPT0WU
NB14 NUHoOI5IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTIXYJKSzVyPUCuNVY1PDdizszN MnP4V2FPT0WU
Ramos-2G6-4C10 MnPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTBwMU[5PFMh|ryP MnvoV2FPT0WU
NBsusSR NGHIUYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTBwMUewNVkh|ryP M{fkS3NCVkeHUh?=
LAMA-84 MnrNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37oV2lEPTB;MD6xPFA{OiEQvF2= NInYWoZUSU6JRWK=
NCI-H1581 NEP4[oJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUnJR|UxRTBwMUi5OlYh|ryP MojjV2FPT0WU
A204 M{LETmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3u3[2lEPTB;MD6xPFk4KM7:TR?= NHXnVpFUSU6JRWK=
A549 NH7YTHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPVPIJwUUN3ME2wMlE6PTVizszN MljUV2FPT0WU
CAL-51 NH;yNmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLXOHZKSzVyPUCuNVk3PTFizszN M17PSnNCVkeHUh?=
NKM-1 NXnZV5V3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NILDcJFKSzVyPUCuNlAxOjlizszN NV\EbVJlW0GQR1XS
LoVo MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzpWZhKSzVyPUCuNlAzODJizszN NYTPW2xGW0GQR1XS
HAL-01 M37UNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjaTWM2OD1yLkKwNlk4KM7:TR?= NYPPXGl1W0GQR1XS
HCC2218 NHzvSYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTBwMkC2PFgh|ryP NEi1dpFUSU6JRWK=
BEN NVu2bmdoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWT2VVNuUUN3ME2wMlIyOjl|IN88US=> MnvIV2FPT0WU
LC-2-ad MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX2wV3JxUUN3ME2wMlIzODh2IN88US=> M3;jOnNCVkeHUh?=
LXF-289 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rJT2lEPTB;MD6yOVA1OSEQvF2= NGnNVFNUSU6JRWK=
HGC-27 MlLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljMTWM2OD1yLkK2OFE1KM7:TR?= NI\WflBUSU6JRWK=
LK-2 M4fWZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXhUpRKSzVyPUCuNlY5OjRizszN M2r6VHNCVkeHUh?=
CTB-1 MkPTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fxO2lEPTB;MD6yO|IyPSEQvF2= Ml7WV2FPT0WU
697 Mn\ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwMkezOlch|ryP MX\TRW5ITVJ?
ES7 Mo[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTBwMkezPFMh|ryP MlKyV2FPT0WU
MG-63 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWL4OGl3UUN3ME2wMlI4PDB5IN88US=> Mn\RV2FPT0WU
T47D NWn1PIhoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTBwMke0O|Uh|ryP NHzJe5hUSU6JRWK=
RPMI-8226 M1TkZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml[5TWM2OD1yLkK4OVg6KM7:TR?= NUH5b2MxW0GQR1XS
NCI-H292 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPlW5NKSzVyPUCuNlkyPjRizszN NHzH[|hUSU6JRWK=
HT Mn76S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\lSGVKSzVyPUCuNlk{QCEQvF2= MoCyV2FPT0WU
A3-KAW MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTBwMkm3OEDPxE1? NVvYUnhpW0GQR1XS
LB2241-RCC MmjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTBwM{CxPVgh|ryP MXvTRW5ITVJ?
BB65-RCC NXTMZZJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nuc2lEPTB;MD6zNFgxPSEQvF2= NXHGOoRMW0GQR1XS
G-401 NXX1VJBrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTBwM{C5PFYh|ryP MoLrV2FPT0WU
TYK-nu MlHBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXLR4FXUUN3ME2wMlMyQDZ2IN88US=> MXzTRW5ITVJ?
NCI-SNU-1 MkXWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTBwM{KzN|Eh|ryP NIKyTIxUSU6JRWK=
CHL-1 NUHGdZZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPCe5FIUUN3ME2wMlMzPzB|IN88US=> MVfTRW5ITVJ?
P30-OHK NYfHbnF1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHpTWM2OD1yLkOyO|A2KM7:TR?= NF;IcnpUSU6JRWK=
PA-1 M3Htd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTBwM{O2NVkh|ryP MX\TRW5ITVJ?
LB1047-RCC NVz5OY51T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfZ[GtTUUN3ME2wMlM1ODR5IN88US=> NH7rUJRUSU6JRWK=
EM-2 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELUUWNKSzVyPUCuN|QyQDhizszN NVvaRmNxW0GQR1XS
VA-ES-BJ NI\QSVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfZblhKSzVyPUCuN|QzPjhizszN M4PCRnNCVkeHUh?=
NCI-H520 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTBwM{S4N|Qh|ryP NYn4Z2xDW0GQR1XS
ECC10 M2XkVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGLENFZKSzVyPUCuN|UxOzVizszN MWLTRW5ITVJ?
LNCaP-Clone-FGC NYK5XoVKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3sSXR[UUN3ME2wMlM2OzF2IN88US=> NX;tPGluW0GQR1XS
ES8 Mn7zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTBwM{[1OVMh|ryP NVTHNZoyW0GQR1XS
HuO-3N1 NGW0SJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTBwM{izO|kh|ryP MUDTRW5ITVJ?
L-363 NGLQ[49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmX5TWM2OD1yLkO5NFI3KM7:TR?= MljIV2FPT0WU
SK-LU-1 MoC1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jaN2lEPTB;MD6zPVIxQCEQvF2= MnvYV2FPT0WU
MCF7 NFS5dmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rSd2lEPTB;MD60NFU{KM7:TR?= NUDufWFrW0GQR1XS
HuO9 NXm5Sm1oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3r2SGlEPTB;MD60NFY3PyEQvF2= NWTTdZc2W0GQR1XS
BHT-101 NFLxeWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofUTWM2OD1yLkSwO|g4KM7:TR?= NXzO[|ZIW0GQR1XS
MZ2-MEL M1;keGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDWN4k2UUN3ME2wMlQxQDh{IN88US=> NGjp[ZVUSU6JRWK=
NOS-1 M2L1fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTBwNEGwO|Qh|ryP MUTTRW5ITVJ?
NEC8 NF3y[llIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGP5R41KSzVyPUCuOFE3QTVizszN MUfTRW5ITVJ?
COR-L88 Mon4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4KwcmlEPTB;MD60NlI3PyEQvF2= M{XCO3NCVkeHUh?=
MFM-223 NVO1SGMxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGizUG9KSzVyPUCuOFI3OTdizszN M4rOdXNCVkeHUh?=
HSC-3 NHu3N3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4S0OGlEPTB;MD60NlkxQCEQvF2= MkTvV2FPT0WU
OVCAR-5 MojPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVy1[HlxUUN3ME2wMlQzQTN|IN88US=> MoXlV2FPT0WU
MFE-280 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofkTWM2OD1yLkSzNFMzKM7:TR?= Ml\CV2FPT0WU
KP-N-YS MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTBwNEOwO{DPxE1? NFnNXoZUSU6JRWK=
EPLC-272H MojmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\VOnFKSzVyPUCuOFMxPzdizszN NGjrZ4FUSU6JRWK=
KE-37 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTBwNEOyOFch|ryP MmfVV2FPT0WU
HuH-7 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYewSos5UUN3ME2wMlQ{Ozd{IN88US=> NF36RZNUSU6JRWK=
CHP-212 M1PUcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEL1[JlKSzVyPUCuOFM3QTdizszN MlTUV2FPT0WU
SK-MES-1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHf2c5hKSzVyPUCuOFM5PDhizszN M3XhS3NCVkeHUh?=
SJSA-1 Ml3jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3O5Z2lEPTB;MD60OFU2PCEQvF2= MkTTV2FPT0WU
QIMR-WIL MlP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\GUFdKSzVyPUCuOFUyPSEQvF2= M1r6NHNCVkeHUh?=
ES4 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLse2ZKSzVyPUCuOFU{OzZizszN M4PLTXNCVkeHUh?=
TI-73 NHOzTotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTBwNEW2PFYh|ryP NVnwNpRrW0GQR1XS
COLO-684 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmS0TWM2OD1yLkS1O|I3KM7:TR?= M3[zTXNCVkeHUh?=
GCIY NWPa[oxqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnhWndKSzVyPUCuOFU4PTRizszN MonaV2FPT0WU
JVM-3 NVuyd2NWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTw[FhOUUN3ME2wMlQ3Ojl{IN88US=> NHnCdGJUSU6JRWK=
MPP-89 M1TmNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4X5ZmlEPTB;MD60Olk1PyEQvF2= MUfTRW5ITVJ?
M14 NH3mcGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHjRlBHUUN3ME2wMlQ4OTdizszN NY\t[YtLW0GQR1XS
SW962 NIrjO5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\XW|BUUUN3ME2wMlQ4OTlizszN NUTWVop6W0GQR1XS
VM-CUB-1 Mo\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M132WGlEPTB;MD60O|I5PiEQvF2= MXXTRW5ITVJ?
A101D M4m1ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTBwNEi5Olgh|ryP NFzWcoVUSU6JRWK=
DB MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDNTWM2OD1yLkWwNFIyKM7:TR?= MUPTRW5ITVJ?
KNS-62 M{DFeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vSeGlEPTB;MD61NFU3QCEQvF2= NHu2dmxUSU6JRWK=
NCI-H460 NILXN|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTBwNUC1PVch|ryP NH:4TnJUSU6JRWK=
MDA-MB-361 NH;aR4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXwbVVKSzVyPUCuOVA5PyEQvF2= MlXaV2FPT0WU
DOK NIjneI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoH2TWM2OD1yLkWxN|M{KM7:TR?= M3XQVXNCVkeHUh?=
CAL-39 NEHveXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPUTWM2OD1yLkWyNlY5KM7:TR?= NXX4NJdsW0GQR1XS
HT-1080 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rtb2lEPTB;MD61NlU1OyEQvF2= NFzrO2tUSU6JRWK=
GR-ST M1PuSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PXW2lEPTB;MD61N|AyPSEQvF2= NVTJem04W0GQR1XS
J-RT3-T3-5 M1;ydGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjYTJkyUUN3ME2wMlU{OTl3IN88US=> MV\TRW5ITVJ?
NCI-H1793 MkHzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlvXTWM2OD1yLkWzOFQ4KM7:TR?= NUeydZY1W0GQR1XS
NCI-H1355 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPucnM4UUN3ME2wMlU{QDd6IN88US=> NXHQe3dQW0GQR1XS
YH-13 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fDfGlEPTB;MD61OFk{QCEQvF2= M2\GRXNCVkeHUh?=
LOXIMVI NFjDdJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLNTWM2OD1yLkW1NVI{KM7:TR?= NVfOSmxKW0GQR1XS
ES1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrPXW02UUN3ME2wMlU2PTF6IN88US=> M1XxOHNCVkeHUh?=
GAMG NXnYe|k4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7VcXVSUUN3ME2wMlU2PjV6IN88US=> NInvbYdUSU6JRWK=
SK-MEL-28 NFHad2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVOxZmdIUUN3ME2wMlU2QTFizszN M1zjXXNCVkeHUh?=
KG-1 NFPP[4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7LPGJzUUN3ME2wMlU3QDh2IN88US=> MX;TRW5ITVJ?
HOS MkO4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nVTWlEPTB;MD61O|E6OiEQvF2= M{mybXNCVkeHUh?=
PANC-08-13 NEfPPXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTBwNUezPFkh|ryP M2q5O3NCVkeHUh?=
SW780 NVvWd3Q1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTBwNUe1PVUh|ryP NFTSPHFUSU6JRWK=
MDA-MB-175-VII Ml3xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXS1OoNLUUN3ME2wMlU4PzRzIN88US=> Ml72V2FPT0WU
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OVCAR-8 NWDDNIJiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoCwTWM2OD1{LkCwNlk2KM7:TR?= MY\TRW5ITVJ?
DSH1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\yNWNKSzVyPUKuNFE4PzRizszN MX3TRW5ITVJ?
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RCC10RGB MontS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3qySmlEPTB;Mj6wOFg4OSEQvF2= M1v5O3NCVkeHUh?=
SW954 NEjXWVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILvO29KSzVyPUKuNFUxOzZizszN MWPTRW5ITVJ?
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AU565 NX;JfpNOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnP3TWM2OD1{LkC1PVA2KM7:TR?= MnXGV2FPT0WU
CW-2 M3LsWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTJwME[4O|Uh|ryP NITq[lZUSU6JRWK=
TE-1 MlnrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTJwMEmzO|ch|ryP NFHNNlNUSU6JRWK=
KP-4 NXL5ZWNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7JZ3U4UUN3ME2yMlExOzF{IN88US=> MnTIV2FPT0WU
SNU-C2B NVfwRWxbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrsOWt[UUN3ME2yMlExQDJ|IN88US=> NFrtRo9USU6JRWK=
NCI-H1395 MnfXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljBTWM2OD1{LkGwPVg3KM7:TR?= NWr3WmV{W0GQR1XS
CAS-1 MlXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jYPWlEPTB;Mj6xNVU1PiEQvF2= MlLaV2FPT0WU
NCI-H1155 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTJwMkK2PUDPxE1? NWfxN2lbW0GQR1XS
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KINGS-1 M13vemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LyUmlEPTB;Mj6zNFA5QCEQvF2= MYTTRW5ITVJ?
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HT-1376 MlryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;2ZWlEPTB;Mj6zPFcxQCEQvF2= MWPTRW5ITVJ?
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HuCCT1 M3XuNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFHRXm9KSzVyPUKuOVY{QSEQvF2= M17XdnNCVkeHUh?=
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NCI-H1666 NFzx[5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LSXWlEPTB;Mj61PVA4PiEQvF2= MW\TRW5ITVJ?
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EW-13 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGC2R|BKSzVyPUKuOlg4PjhizszN MlOwV2FPT0WU
MLMA NVTSTZM5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfGTWM2OD1{Lk[4PFU3KM7:TR?= NYrzbVFpW0GQR1XS
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RD MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofGTWM2OD1{Lke5OFcyKM7:TR?= NI\PZoRUSU6JRWK=
GT3TKB NEnPb49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\0cWlEPTB;Mj64N|Q{OyEQvF2= NX74dm5YW0GQR1XS
MZ7-mel MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW[yOFBUUUN3ME2yMlg3PTB{IN88US=> MWTTRW5ITVJ?
KYSE-510 NY[3[G9qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTJwOEm1NFQh|ryP NYrISW5rW0GQR1XS
NCI-N87 Mn;CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37aRWlEPTB;Mj65NVgxPyEQvF2= M3n6ZXNCVkeHUh?=
MKN1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkH5TWM2OD1|LkCwNVY{KM7:TR?= MlnRV2FPT0WU
NCI-H2170 NV\VNWU5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTNwMEC0OVEh|ryP MWjTRW5ITVJ?
OVCAR-3 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXf5O3BIUUN3ME2zMlAzOTF3IN88US=> NGPETY5USU6JRWK=
KLE MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fpTGlEPTB;Mz6wOlgyOiEQvF2= NEXC[2RUSU6JRWK=
LB771-HNC MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTUTWM2OD1|LkC5N|Q{KM7:TR?= M3HFcXNCVkeHUh?=
MC116 MlfjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLvOJlVUUN3ME2zMlEyOTF{IN88US=> M3mwUHNCVkeHUh?=
SCC-9 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHXPXFKSzVyPUOuNVE{PjZizszN NETHXnBUSU6JRWK=
U031 MkHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonBTWM2OD1|LkGyOFI{KM7:TR?= NHXiZWxUSU6JRWK=
LCLC-103H M3:5dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDobGpKSzVyPUOuNlM5QTRizszN MUfTRW5ITVJ?
U-266 M4LmUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXocGh3UUN3ME2zMlI4PjN|IN88US=> NF3ve4lUSU6JRWK=
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UACC-257 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MniyTWM2OD1|LkS2OFMyKM7:TR?= MnXxV2FPT0WU
LB996-RCC M4nt[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4mySWlEPTB;Mz60OlUxOiEQvF2= MlP1V2FPT0WU
BT-549 M{DoV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXINmxKSzVyPUOuOVAzODlizszN M{HEcXNCVkeHUh?=
NCI-H526 MkLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17VeWlEPTB;Mz61OFE2QCEQvF2= MYrTRW5ITVJ?
RH-18 NYDqVVU3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTNwNkG3PVgh|ryP NGO0TG1USU6JRWK=
SW626 Mnj3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTNwN{GxNlgh|ryP NHvaNHRUSU6JRWK=
SK-N-AS NWridWpiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHnXpdKSzVyPUOuO|MzPDdizszN NUfne3pCW0GQR1XS
IGR-1 MlSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjtdYRGUUN3ME2zMlc1OjR6IN88US=> NHyxO|dUSU6JRWK=
NCI-H2291 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlv4TWM2OD1|Lke1N|M3KM7:TR?= MoXSV2FPT0WU
NCI-H441 NWTZSY86T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrjTWM2OD1|LkiyOFM{KM7:TR?= M1rlU3NCVkeHUh?=
D-247MG MnPCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTNwOE[wN|Eh|ryP M2rLOXNCVkeHUh?=
COLO-320-HSR MljPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTNwOUGyO|Eh|ryP MW\TRW5ITVJ?
A253 NXTyZplmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTNwOUG0N|Yh|ryP M3\MO3NCVkeHUh?=
HT55 Mn\iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3n1dmlEPTB;Mz65NVc4OyEQvF2= MWPTRW5ITVJ?
MZ1-PC Ml3QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3u2OmlEPTB;Mz65OVg3PCEQvF2= MXrTRW5ITVJ?
RERF-LC-MS NVfxNoRpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTNwOU[1NlUh|ryP Mn72V2FPT0WU
SF268 NUPJVowzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHIVnNbUUN3ME20MlEzQDhzIN88US=> NHnORY9USU6JRWK=
RMG-I M3fJWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTRwMkG0OVQh|ryP MYLTRW5ITVJ?
CFPAC-1 NGH0[4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXZT4VKSzVyPUSuNlE3QDVizszN MoDSV2FPT0WU
S-117 NH;L[JJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37ERmlEPTB;ND6yNlAzPCEQvF2= MVLTRW5ITVJ?
NCI-H2122 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTRwM{C5N|Eh|ryP NIHVRmJUSU6JRWK=
J82 M{DwXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XlTmlEPTB;ND6zN|Q1OSEQvF2= NY\VZ49wW0GQR1XS
CAL-72 NF;wOnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfjXIk6UUN3ME20MlM2PDd5IN88US=> MUHTRW5ITVJ?
GP5d Moj3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7kfItKSzVyPUSuOFEzQThizszN NYnpV28{W0GQR1XS
UACC-62 NH:2[HRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEm2[plKSzVyPUSuOFY2PDVizszN NI\LRZpUSU6JRWK=
MS-1 NVPaXohKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MorKTWM2OD12LkS5NVE2KM7:TR?= NX3qXFl6W0GQR1XS
RCM-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTRwNUK5NkDPxE1? NXi4cVFGW0GQR1XS
IST-SL1 M17nc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HZ[mlEPTB;ND62OVM2QSEQvF2= MU\TRW5ITVJ?
SW756 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVP5XHhoUUN3ME20MlY6OTN3IN88US=> M2C0OXNCVkeHUh?=
HCC38 M4nuWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTRwN{GxPVch|ryP M2Hve3NCVkeHUh?=
YAPC M3zRRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3BfnNKSzVyPUSuO|E{OTNizszN MlLZV2FPT0WU
DK-MG MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\DO3pKSzVyPUSuO|gyOTJizszN MkjEV2FPT0WU
HCC1937 NHnpU|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LrdGlEPTB;ND64OVEzPSEQvF2= MmrVV2FPT0WU
OAW-28 M{ixd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTpfoVKSzVyPUSuPVI1OzVizszN MVfTRW5ITVJ?
MEG-01 NXzxSWV4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTVwMEizNlgh|ryP MYTTRW5ITVJ?
GI-1 NEHMb2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TI[WlEPTB;NT6yN|Q5PiEQvF2= M3jYdHNCVkeHUh?=
FADU M1PzOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4TudWlEPTB;NT6yO|g{PyEQvF2= NXHwbZNUW0GQR1XS
CAPAN-1 M4jIemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLEdXhrUUN3ME21MlM6PDN{IN88US=> M2HtSHNCVkeHUh?=
TGBC11TKB MoLrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3EWYJPUUN3ME21MlQ2Pzh4IN88US=> M3r0THNCVkeHUh?=
DBTRG-05MG NUO1VXNkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\meYVKSzVyPUWuOVA2OiEQvF2= M1v6eXNCVkeHUh?=
ONS-76 M171Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoiyTWM2OD13LkW1PFAyKM7:TR?= NEjIVYFUSU6JRWK=
Ca-Ski MkLWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTSNHVHUUN3ME21MlYzPTZ4IN88US=> MUPTRW5ITVJ?
COLO-741 MmLNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\ROHJHUUN3ME21MlcyQTl3IN88US=> MUPTRW5ITVJ?
HT-29 MmHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnGTWM2OD13LkiwOFc2KM7:TR?= MWrTRW5ITVJ?
HCC1187 NVjoXFNlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnm3TWM2OD13LkixNFA5KM7:TR?= NYLYd2NXW0GQR1XS
Capan-2 MkT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInEOWlKSzVyPUWuPVU6QTRizszN NV;1XmZHW0GQR1XS
NCI-H630 NFn0[5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;R[2lEPTB;Nj6xNVQ1OSEQvF2= NIr3SlFUSU6JRWK=
COLO-678 M{LiPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnBTWM2OD14LkG1NFkzKM7:TR?= M3fGTHNCVkeHUh?=
D-502MG MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnmWGlKSzVyPU[uNVc{OzZizszN MY\TRW5ITVJ?
NB17 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjo[GlKSzVyPU[uNlQ6PDNizszN M2jUd3NCVkeHUh?=
SK-MEL-24 M33kR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTZwMk[0PUDPxE1? NV;hWnBnW0GQR1XS
NCI-SNU-5 M2\6e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXK[GhKSzVyPU[uNlcyOThizszN MoLPV2FPT0WU
D-392MG MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PPNGlEPTB;Nj6zN|M6QSEQvF2= M3jOdXNCVkeHUh?=
A498 MmqwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;RTWM2OD14LkSwN|AzKM7:TR?= MXrTRW5ITVJ?
OCI-AML2 MlewS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTwTWM2OD14LkS1OFQ2KM7:TR?= MV3TRW5ITVJ?
HCC2157 NHzXclJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTZwNEW2NVYh|ryP M4nIUnNCVkeHUh?=
C3A MnrHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjjXo9KSzVyPU[uOVIxODRizszN M1;G[3NCVkeHUh?=
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CCRF-CEM M2[zOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nM[2lEPTB;ND6xJI5O NXLRb3l5OjF|M{e2O|k>
Kasumi-1 NYiyXYw{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXUTWM2OD1zLkSgcm0> MoXTNlE{Ozd4N{m=
Karpas-299 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmmxTWM2OD1{MT63JI5O NYrUS5lFOjF|M{e2O|k>
Ramos-RA1 M2r3PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{P4[WlEPTB;OD6yJI5O NWLMemxCOjF|M{e2O|k>
H838 NH7PWINHfW6ldHnvckBCe3OjeR?= M1PwbFEh|ryP NIn4bWw1QCCq NIjFTm9qdmS3Y3XzJINmdGxiYYX0c5Bp[We7 NY\0bYpCOjB|NkSxNVM>
H838 NGfROpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HrW|AuOyEQvF2= NWjIfFdoPzJiaB?= NV3GUJRlcW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 MoXvNlAxOjh6NUS=
A549  MmWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYCwMVMh|ryP NXLKN4FYPzJiaB?= M{LhNIlv\HWlZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKGG3dH;wbIFogQ>? MkGyNlAxOjh6NUS=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; 

PubMed: 24457069     


Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙

p-EGFR / EGFR; 

PubMed: 25654224     


PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot;

p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; 

PubMed: 25654224     


PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.

24457069 25654224
Immunofluorescence
p-4EBP1 / EIF4E / mTOR; 

PubMed: 24600487     


(C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋€𢡄�෋€䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemセ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒セ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wセ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v 玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴￿￿嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗

LC3B / p62 ; 

PubMed: 30337536     


Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞

24600487 30337536
Growth inhibition assay
Cell viability ; 

PubMed: 29263324     


Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.

29263324
In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
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Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
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  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A
Smiles COC1=C(CO)C=C(C=C1)C2=NC3=C(C=C2)C(=NC(=N3)N4CCOCC4C)N5CCOCC5C

Bio Calculators

Molarity Calculator

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID