AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 40 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

  • HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

    Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017, 15(1):9. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 NV:5XZN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTBwMEKzN|Eh|ryP M{nqbXNCVkeHUh?=
NCI-H720 M1P0bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3\EZ2lEPTB;MD6wNlk{QCEQvF2= NWe4e5R3W0GQR1XS
KARPAS-45 NGTlRVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTBwMEOzO|Eh|ryP NGHF[nhUSU6JRWK=
EW-3 NIP6coJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDBXGVIUUN3ME2wMlA1ODV4IN88US=> NIDyRlVUSU6JRWK=
SBC-1 NVz6bXMyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHYXmtKSzVyPUCuNFQ2QDFizszN NGC3ZoNUSU6JRWK=
NCI-H209 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTBwMEW0OVMh|ryP MV3TRW5ITVJ?
EoL-1-cell MkTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTNTWM2OD1yLkC2OVY6KM7:TR?= NF3MXGZUSU6JRWK=
H9 MlrVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXFW3RKSzVyPUCuNFY3QTJizszN M3j4OHNCVkeHUh?=
AN3-CA Mk[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLkTWM2OD1yLkC3PVUyKM7:TR?= MmXQV2FPT0WU
HH M1qwbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3jGVWlEPTB;MD6wPFAxQSEQvF2= NFLv[mdUSU6JRWK=
KU812 Mki1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrYTWM2OD1yLkC4NFQ{KM7:TR?= MoHpV2FPT0WU
DU-4475 M4DFTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDGN4VSUUN3ME2wMlA5ODR5IN88US=> M3WyWXNCVkeHUh?=
CTV-1 MlnHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvzVHFKSzVyPUCuNFg3PDFizszN MU\TRW5ITVJ?
BV-173 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zZN2lEPTB;MD6wPVA3OSEQvF2= NHTM[pVUSU6JRWK=
ES5 NFPqTW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTnTWM2OD1yLkC5N|Uh|ryP NVXXPJIyW0GQR1XS
G-402 MorvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDrTWM2OD1yLkC5PVgh|ryP NV7CeHE3W0GQR1XS
MV-4-11 NYXjSplbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXMRoFKSzVyPUCuNVA{PDdizszN MVLTRW5ITVJ?
NB69 NHX1eoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTBwMUO1JO69VQ>? MYXTRW5ITVJ?
EGI-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofJTWM2OD1yLkGzOVMyKM7:TR?= NETWd|JUSU6JRWK=
EW-18 M4P6[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIfyVXZKSzVyPUCuNVQxPzNizszN NGLXWIpUSU6JRWK=
ME-180 M1vMV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYC1TVJxUUN3ME2wMlE1PjhzIN88US=> MXXTRW5ITVJ?
D-283MED MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rkZmlEPTB;MD6xOFczOyEQvF2= MkS5V2FPT0WU
NB14 M2Trdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLMTWM2OD1yLkG2OFQ4KM7:TR?= Mmm1V2FPT0WU
Ramos-2G6-4C10 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrnNVZPUUN3ME2wMlE3QTh|IN88US=> MnzLV2FPT0WU
NBsusSR MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;1Xm5xUUN3ME2wMlE4ODF7IN88US=> NF6ySlZUSU6JRWK=
LAMA-84 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrFfGVKSzVyPUCuNVgxOzJizszN NEnFXVZUSU6JRWK=
NCI-H1581 NIThWnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPHdXF4UUN3ME2wMlE5QTZ4IN88US=> MkHRV2FPT0WU
A204 M{HveGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofWTWM2OD1yLkG4PVch|ryP MWjTRW5ITVJ?
A549 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DaVmlEPTB;MD6xPVU2KM7:TR?= M{fBXXNCVkeHUh?=
CAL-51 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXz2XWF7UUN3ME2wMlE6PjVzIN88US=> NFLue3NUSU6JRWK=
NKM-1 NGjtR4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTBwMkCwNlkh|ryP MnHpV2FPT0WU
LoVo M1XKVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnTUTWM2OD1yLkKwNlAzKM7:TR?= NY\tXI1wW0GQR1XS
HAL-01 NIjBc45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rFdmlEPTB;MD6yNFI6PyEQvF2= NVLRe2xHW0GQR1XS
HCC2218 MljlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHtZmhKSzVyPUCuNlA3QDhizszN NWTDTZBEW0GQR1XS
BEN MlrtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPMTWM2OD1yLkKxNlk{KM7:TR?= NUGyNHo3W0GQR1XS
LC-2-ad MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXDPFJXUUN3ME2wMlIzODh2IN88US=> NF7mZolUSU6JRWK=
LXF-289 M2TSVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHWT3hDUUN3ME2wMlI2ODRzIN88US=> NX;4fVN{W0GQR1XS
HGC-27 MkSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWPmcWtvUUN3ME2wMlI3PDF2IN88US=> NYXLOHlEW0GQR1XS
LK-2 M{H2Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEm5cFRKSzVyPUCuNlY5OjRizszN NUPDeWppW0GQR1XS
CTB-1 M{jOPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTBwMkeyNVUh|ryP M{HJWHNCVkeHUh?=
697 NIH1[VRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\5cmlEPTB;MD6yO|M3PyEQvF2= MXPTRW5ITVJ?
ES7 MnzxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TjW2lEPTB;MD6yO|M5OyEQvF2= MXHTRW5ITVJ?
MG-63 Mn7VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\yNGlEPTB;MD6yO|QxPyEQvF2= MV3TRW5ITVJ?
T47D MoLpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXG4V|ZUUUN3ME2wMlI4PDd3IN88US=> MoSwV2FPT0WU
RPMI-8226 MnP4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHP3fmVKSzVyPUCuNlg2QDlizszN Mo\nV2FPT0WU
NCI-H292 Mnn4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTBwMkmxOlQh|ryP M4rKeXNCVkeHUh?=
HT MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHHXYl6UUN3ME2wMlI6OzhizszN M3HJSnNCVkeHUh?=
A3-KAW NGTMdJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLld|BKSzVyPUCuNlk4PCEQvF2= MXfTRW5ITVJ?
LB2241-RCC Mn6yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTBwM{CxPVgh|ryP NV\s[JJUW0GQR1XS
BB65-RCC MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfHeIRjUUN3ME2wMlMxQDB3IN88US=> MV\TRW5ITVJ?
G-401 NFrzRoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{j2Z2lEPTB;MD6zNFk5PiEQvF2= MVfTRW5ITVJ?
TYK-nu NVPrO3hkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XIRmlEPTB;MD6zNVg3PCEQvF2= M3n3bHNCVkeHUh?=
NCI-SNU-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPvPWJ5UUN3ME2wMlMzOzNzIN88US=> M{nXN3NCVkeHUh?=
CHL-1 NWTL[ldYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIL6W|JKSzVyPUCuN|I4ODNizszN Ml\JV2FPT0WU
P30-OHK MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4n6bmlEPTB;MD6zNlcxPSEQvF2= NXX3N3FlW0GQR1XS
PA-1 Mn7NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHBbWdKSzVyPUCuN|M3OTlizszN NEPwVo5USU6JRWK=
LB1047-RCC MmnDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFKw[ndKSzVyPUCuN|QxPDdizszN M3LEN3NCVkeHUh?=
EM-2 NWLiSGo2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3zTWM2OD1yLkO0NVg5KM7:TR?= MV\TRW5ITVJ?
VA-ES-BJ NGHXflNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnP4TWM2OD1yLkO0NlY5KM7:TR?= MmHmV2FPT0WU
NCI-H520 M2jVemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTBwM{S4N|Qh|ryP MWfTRW5ITVJ?
ECC10 NWToS2tqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDsTWM2OD1yLkO1NFM2KM7:TR?= MU\TRW5ITVJ?
LNCaP-Clone-FGC MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2D5[2lEPTB;MD6zOVMyPCEQvF2= MmnSV2FPT0WU
ES8 NWHDbnhZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPITWM2OD1yLkO2OVU{KM7:TR?= MWjTRW5ITVJ?
HuO-3N1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPwTWM2OD1yLkO4N|c6KM7:TR?= MmPLV2FPT0WU
L-363 MmDhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTBwM{mwNlYh|ryP M{OybHNCVkeHUh?=
SK-LU-1 M4r1bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnMfZNtUUN3ME2wMlM6OjB6IN88US=> MknWV2FPT0WU
MCF7 NILrXIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrGdXlKSzVyPUCuOFA2OyEQvF2= M3HGOXNCVkeHUh?=
HuO9 MkTJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3r6d2lEPTB;MD60NFY3PyEQvF2= M3L3VXNCVkeHUh?=
BHT-101 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTBwNEC3PFch|ryP NUXJUpF1W0GQR1XS
MZ2-MEL MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX75SIpYUUN3ME2wMlQxQDh{IN88US=> M1;ac3NCVkeHUh?=
NOS-1 NHPBPJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTEfY1XUUN3ME2wMlQyODd2IN88US=> M3q4UHNCVkeHUh?=
NEC8 NVL4VJJiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIWyNY9KSzVyPUCuOFE3QTVizszN M2jCXHNCVkeHUh?=
COR-L88 MoezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUOxNo1PUUN3ME2wMlQzOjZ5IN88US=> MYLTRW5ITVJ?
MFM-223 NEG4Vm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGKxRWtKSzVyPUCuOFI3OTdizszN M1;CN3NCVkeHUh?=
HSC-3 NFftTJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3j[FNKSzVyPUCuOFI6ODhizszN NWnmemZGW0GQR1XS
OVCAR-5 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;PTWM2OD1yLkSyPVM{KM7:TR?= M4HHNHNCVkeHUh?=
MFE-280 MmnWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;BTWM2OD1yLkSzNFMzKM7:TR?= NXHoOIViW0GQR1XS
KP-N-YS NVvo[5RzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVH4WYNbUUN3ME2wMlQ{ODdizszN NH7wV4VUSU6JRWK=
EPLC-272H NW\G[lkzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3XV29wUUN3ME2wMlQ{ODd5IN88US=> MXvTRW5ITVJ?
KE-37 MkTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4OwPGlEPTB;MD60N|I1PyEQvF2= M1\2PXNCVkeHUh?=
HuH-7 M1PkNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTBwNEOzO|Ih|ryP NXzZO484W0GQR1XS
CHP-212 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTBwNEO2PVch|ryP M163V3NCVkeHUh?=
SK-MES-1 M{LicWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvtTWM2OD1yLkSzPFQ5KM7:TR?= M374RXNCVkeHUh?=
SJSA-1 M3ThO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;zOWlEPTB;MD60OFU2PCEQvF2= NHPRR5pUSU6JRWK=
QIMR-WIL M2XnSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;RNWlEPTB;MD60OVE2KM7:TR?= M4XyXXNCVkeHUh?=
ES4 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PQOGlEPTB;MD60OVM{PiEQvF2= MnjvV2FPT0WU
TI-73 Mly3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTBwNEW2PFYh|ryP NGH4[HlUSU6JRWK=
COLO-684 NWPNTWhjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfndVQ6UUN3ME2wMlQ2PzJ4IN88US=> MWHTRW5ITVJ?
GCIY Mlj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;RXWxXUUN3ME2wMlQ2PzV2IN88US=> MV;TRW5ITVJ?
JVM-3 M1;W[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDXTWM2OD1yLkS2NlkzKM7:TR?= M3myRXNCVkeHUh?=
MPP-89 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfFemxXUUN3ME2wMlQ3QTR5IN88US=> NX20T2ZXW0GQR1XS
M14 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTBwNEexO{DPxE1? NVvD[2dyW0GQR1XS
SW962 M{jWXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPRb3dKSzVyPUCuOFcyQSEQvF2= NXTPUVl{W0GQR1XS
VM-CUB-1 NFLSfXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHsPI9oUUN3ME2wMlQ4Ojh4IN88US=> MX7TRW5ITVJ?
A101D M2\Sbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPMTWM2OD1yLkS4PVY5KM7:TR?= MYLTRW5ITVJ?
DB M{[z[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkS5TWM2OD1yLkWwNFIyKM7:TR?= MV3TRW5ITVJ?
KNS-62 NUXNW4xST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\tbWlEPTB;MD61NFU3QCEQvF2= M1fNTHNCVkeHUh?=
NCI-H460 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTBwNUC1PVch|ryP M4LlfHNCVkeHUh?=
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DOK MnLyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTBbYFSUUN3ME2wMlUyOzN|IN88US=> NYrjfWVnW0GQR1XS
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CAL-54 NUTZSWJ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXvWoxNUUN3ME2xMlg{OzF|IN88US=> MmfiV2FPT0WU
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HCC2998 MlTqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7GVY93UUN3ME2xMlk{OzJ3IN88US=> NUXaZ3c{W0GQR1XS
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OVCAR-8 MkHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HW[WlEPTB;Mj6wNFI6PSEQvF2= MVfTRW5ITVJ?
DSH1 M1zwNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{myd2lEPTB;Mj6wNVc4PCEQvF2= NULjRZI{W0GQR1XS
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RCC10RGB M1\Ge2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnJSlE2UUN3ME2yMlA1QDdzIN88US=> NVHxfpREW0GQR1XS
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NCI-H1395 NWL4Z4JJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fOUWlEPTB;Mj6xNFk5PiEQvF2= MUjTRW5ITVJ?
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HT-1376 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTJwM{i3NFgh|ryP M1fRfHNCVkeHUh?=
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NCI-H810 MmDxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLsSIJqUUN3ME2yMlczOTl6IN88US=> M3rL[nNCVkeHUh?=
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MZ7-mel NW\obmdyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk[3TWM2OD1{Lki2OVAzKM7:TR?= NILwTVJUSU6JRWK=
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NCI-N87 MoTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHYTWM2OD1{LkmxPFA4KM7:TR?= Moj6V2FPT0WU
MKN1 NFzhNYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\0d4hSUUN3ME2zMlAxOTZ|IN88US=> NUX0O2V7W0GQR1XS
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LCLC-103H M2DweWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGmzXmZKSzVyPUOuNlM5QTRizszN NHjMXpBUSU6JRWK=
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LB996-RCC MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XLN2lEPTB;Mz60OlUxOiEQvF2= MonYV2FPT0WU
BT-549 MnzMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTNwNUCyNFkh|ryP NHvDd|hUSU6JRWK=
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RH-18 MlT3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXITWM2OD1|Lk[xO|k5KM7:TR?= MXvTRW5ITVJ?
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SF268 NGD4dmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrxTWM2OD12LkGyPFgyKM7:TR?= MULTRW5ITVJ?
RMG-I MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTRwMkG0OVQh|ryP NWf1cW9iW0GQR1XS
CFPAC-1 NV;z[4FTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTRwMkG2PFUh|ryP MV7TRW5ITVJ?
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NCI-H2122 NXn0WZgyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXkVmtQUUN3ME20MlMxQTNzIN88US=> NVntepBxW0GQR1XS
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CAL-72 NX;lc3hCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLJUoVCUUN3ME20MlM2PDd5IN88US=> NHrzNo5USU6JRWK=
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RCM-1 NYmyV4g1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfiPVBKSzVyPUSuOVI6OiEQvF2= M2\IfnNCVkeHUh?=
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SW756 NIe0OHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnacXVuUUN3ME20MlY6OTN3IN88US=> M4X5PHNCVkeHUh?=
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YAPC MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1i4[mlEPTB;ND63NVMyOyEQvF2= NYTQ[YNIW0GQR1XS
DK-MG M1XIZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHmwV|lKSzVyPUSuO|gyOTJizszN NWHIcoxKW0GQR1XS
HCC1937 MmnLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zJfmlEPTB;ND64OVEzPSEQvF2= NWr1cHpFW0GQR1XS
OAW-28 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHX0cnRKSzVyPUSuPVI1OzVizszN NUfLOVVIW0GQR1XS
MEG-01 MkDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnCVXZ3UUN3ME21MlA5OzJ6IN88US=> NYLLXFhCW0GQR1XS
GI-1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTVwMkO0PFYh|ryP NVj5fGM6W0GQR1XS
FADU MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXuTWM2OD13LkK3PFM4KM7:TR?= MkXTV2FPT0WU
CAPAN-1 NV\L[YJnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TUemlEPTB;NT6zPVQ{OiEQvF2= MkHWV2FPT0WU
TGBC11TKB M1:1O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDITWM2OD13LkS1O|g3KM7:TR?= Mn;XV2FPT0WU
DBTRG-05MG NF;McVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUK2R3l5UUN3ME21MlUxPTJizszN M{frR3NCVkeHUh?=
ONS-76 NHPkUmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTVwNUW4NFEh|ryP M3LEWXNCVkeHUh?=
Ca-Ski MomzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTVwNkK1OlYh|ryP MXPTRW5ITVJ?
COLO-741 NVrUSlVkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;KTWM2OD13LkexPVk2KM7:TR?= NVG5NHJtW0GQR1XS
HT-29 M4XRXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\MOI9IUUN3ME21MlgxPDd3IN88US=> MXLTRW5ITVJ?
HCC1187 M3vpZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljZTWM2OD13LkixNFA5KM7:TR?= M{nDVnNCVkeHUh?=
Capan-2 NH3MVHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXXcGxKSzVyPUWuPVU6QTRizszN NFrHflBUSU6JRWK=
NCI-H630 Mm\RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnT[IpoUUN3ME22MlEyPDRzIN88US=> NFTVR5RUSU6JRWK=
COLO-678 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTnO2RKSzVyPU[uNVUxQTJizszN NFHNOnhUSU6JRWK=
D-502MG NYLEVGRNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTZwMUezN|Yh|ryP MXvTRW5ITVJ?
NB17 MoTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm[5TWM2OD14LkK0PVQ{KM7:TR?= M1OwWHNCVkeHUh?=
SK-MEL-24 NVHWTZVUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2e4eWlEPTB;Nj6yOlQ6KM7:TR?= MXfTRW5ITVJ?
NCI-SNU-5 NH2zbVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3uSHFKSzVyPU[uNlcyOThizszN MW\TRW5ITVJ?
D-392MG NHniU4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTZwM{OzPVkh|ryP M3rpTnNCVkeHUh?=
A498 MnvuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTZwNECzNFIh|ryP MYrTRW5ITVJ?
OCI-AML2 NUL3cpczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTZwNEW0OFUh|ryP MkfJV2FPT0WU
HCC2157 NELMOVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTZwNEW2NVYh|ryP NVvyPWN2W0GQR1XS
C3A M4fmWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3ryRWlEPTB;Nj61NlAxPCEQvF2= MYHTRW5ITVJ?
NCI-H1650 NFfrXm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTZwN{C1NlIh|ryP MlPyV2FPT0WU
DU-145 Ml\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rCfGlEPTB;Nj63O|A{KM7:TR?= NGPjdWFUSU6JRWK=
COLO-829 NUSwT4tVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2jN[GlEPTB;Nj63O|g4OyEQvF2= MnXOV2FPT0WU
LS-1034 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTdwMES0NVIh|ryP NIK2VoZUSU6JRWK=
HCC1954 MknMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFy4PY1KSzVyPUeuOFgyOzRizszN NGjlW5NUSU6JRWK=
KURAMOCHI Ml3wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTdwNEi1N|Mh|ryP NVX6VGF4W0GQR1XS
Mo-T M1jVZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnCbohQUUN3ME23MlUyPDl6IN88US=> NWrQTGlwW0GQR1XS
OE19 NIjjU29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTdwNkW1NFMh|ryP NGXmc5pUSU6JRWK=
COR-L105 MkWxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHjTWM2OD15LkexOFIyKM7:TR?= NYS1fGxSW0GQR1XS
NCI-H2342 NEnYWYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTdwN{G1NlEh|ryP MXrTRW5ITVJ?
U-87-MG NEPicm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn76TWM2OD16LkCzN|M1KM7:TR?= MUDTRW5ITVJ?
MKN28 MoPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3[zZ2lEPTB;OD6xNVUyPSEQvF2= MmjWV2FPT0WU
HT-1197 NVnBcIRTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRThwMkW5NFch|ryP MUXTRW5ITVJ?
ChaGo-K-1 M{XxSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRThwM{SyOFUh|ryP M1zvbXNCVkeHUh?=
NCI-H226 NFLDPXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRThwNESzN|Eh|ryP NF3VZVdUSU6JRWK=
NCI-H2029 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrLN|dKSzVyPUmuNFY4PTlizszN MmXzV2FPT0WU
TE-11 M1nxfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PIOWlEPTB;OT60OVU5QCEQvF2= Moe0V2FPT0WU
ZR-75-30 NWH2ZYdrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljRTWM2OD17LkmyOlM1KM7:TR?= M1LIc3NCVkeHUh?=
SW837 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXqTWM2OD1zMD6yNlk{KM7:TR?= MVXTRW5ITVJ?
NCI-H1651 NX:wUFNDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvJSoJKSzVyPUGwMlI{OjZizszN NFe0[GdUSU6JRWK=
NCI-H727 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;2fYJKSzVyPUGwMlQ1QTVizszN MkmwV2FPT0WU
NCI-H358 NXvzXFFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnFXVdKSzVyPUGwMlg{PjVizszN NG\XPIpUSU6JRWK=
NCI-H2087 NGPWUlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTFzLkC0N|Qh|ryP MUDTRW5ITVJ?
FTC-133 NFnUUI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGe4bnpKSzVyPUGxMlQxPTFizszN Ml;nV2FPT0WU
LAN-6 MkHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTF{LkGyOEDPxE1? M1HTdXNCVkeHUh?=
NCI-H1838 NWnJTWN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknYTWM2OD1zMj61NVUyKM7:TR?= MXXTRW5ITVJ?
Calu-6 MnfPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn:xTWM2OD1zND6xNFIyKM7:TR?= MnPyV2FPT0WU
HCC70 M4P3VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDmeYJkUUN3ME2xOE41PTFizszN MkDGV2FPT0WU
KARPAS-299 Mne2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1:5bmlEPTB;MUWuNlE2QSEQvF2= NX70W4hXW0GQR1XS
CAL-27 M4\oV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfYRZhEUUN3ME2xOU4{QDR|IN88US=> M330TnNCVkeHUh?=
NCI-H82 NYX5OY5TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXlTWM2OD1zNT61N|Ih|ryP MnHOV2FPT0WU
no-10 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{f2NGlEPTB;MUWuPVM5KM7:TR?= M4fFenNCVkeHUh?=
U251 MnXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33GWGlEPTB;MU[uN|Y4PSEQvF2= NVnZbGRoW0GQR1XS
TE-9 MmrMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIr3WHRKSzVyPUG3MlUyPjZizszN NHexWGdUSU6JRWK=
TE-10 NFHNO2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkL1TWM2OD1zOT6wOlA4KM7:TR?= NWPXSW8xW0GQR1XS
COLO-824 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWH6WZNLUUN3ME2yNE46Ojd4IN88US=> NEX3dVZUSU6JRWK=
HuP-T3 M2O5VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTJzLkC3OFgh|ryP NVXFe3JKW0GQR1XS
KALS-1 NGexPHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3fDRmlEPTB;MkGuNVc2KM7:TR?= NFvHSI9USU6JRWK=
L-428 M{n0Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\p[I54UUN3ME2yNU41ODF4IN88US=> M1nJUXNCVkeHUh?=
JAR MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjJeVRVUUN3ME2yNU42ODJizszN MXHTRW5ITVJ?
DoTc2-4510 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7kcHJZUUN3ME2yN{45PDd3IN88US=> MoHXV2FPT0WU
ABC-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTJ4Lke5OVch|ryP NVvJW2x4W0GQR1XS
MHH-NB-11 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUT4UId[UUN3ME2zN{4{PDZ2IN88US=> NEPDc|RUSU6JRWK=
SW1463 NUnrO4JQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3iTWM2OD1|Mz65PFMh|ryP NUWzPVROW0GQR1XS
SW1417 MlPsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlH4TWM2OD1|Nz6wPVUzKM7:TR?= M1exRXNCVkeHUh?=
NB12 NXjYVoM5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TxT2lEPTB;M{muOFY2PSEQvF2= MV;TRW5ITVJ?
NY NIPm[3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\4TWM2OD12MT6zNlAyKM7:TR?= MYTTRW5ITVJ?
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H446 NFXT[XhCeG:ydH;zbZMhSXO|YYm= MV:1NFAhdk1? MWi3NkBp MoH6cohidmOnczDBRnQuOjZ|4pETbY5lfWOnZDDhdI9xfG:|aYO= NE\ENZIzPTd|N{W0Ni=>
TFK-1 MlXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfLPIY4OiCq NVnYNIpKTE2VTx?= NWW4UoFIUUN3ME20O{BvVQ>? NUTUPGx5OjVzNUKyOFQ>
EGI-1 NHHEfFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUThN4k2PzJiaB?= NH3PWlZFVVOR NUfESnlwUUN3ME21N{BvVQ>? NEfXe|kzPTF3MkK0OC=>
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MOLM13 NV\mXFFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXj3U3U6OC5{IN88US=> MUC0PEBp MX\lcohidmOnczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGmwZIXj[YQh[nlic3XseY1mfGmwaXK= MX[yOFc{QTN7Mx?=
MCF7 NFjUS4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fy[FIxKG6P MmrsO|IhcA>? NYniPYI4TkKV NF7oWohz\WS3Y3XzJJRp\SCSRVzQNU1u\WSrYYTl[EBqdmO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fS=> M3TxfFI1Pjh6MES2
TamR NEPsOFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFS5PYdKSzVyPUG45qCKdk1? NIn0NokzPDR3N{C2PS=>
MCF7-X  NWDVV4x7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTabopKUUN3ME2yOEBvVQ>? MXuyOFQ2PzB4OR?=
T47D-tamR  NX;tV5BXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PaPGlEPTB;MUmgcm0> NIPkZlAzPDR3N{C2PS=>
293T  MWXGeY5kfGmxbjDBd5NigQ>? MkH5NlAhdk1? MVqzJIg> M3L0SYRqe3K3cITzJGFsfC2vZXTpZZRm\CCWSV[tTWEhe3SjYnnsbZR6NCC2cnHud4xw[2G2aX;uMEBidmRiYXP0bZZqfHl? MXOyOFM3OzR2OR?=
SW620 M4nwbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TKSFAvODBzLUGw{txO M37aflI1KGh? MYDpcohq[mm2czD0bIUh[2WubDDwdo9tcW[ncnH0bY9vKGG|IITo[UBkd26lZX70doF1cW:wIILpd4V{ MkL1NlQ{PjN2NEm=
Huh7  MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFq2XY02NTFyMDDuUS=> MnPGNE0zPCCq NV\FcXI2TE2VTx?= MVrpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> M3Hkd|I1Ojl5M{Cw
Hep3B  MlvuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HHV|UuOTByIH7N MYqwMVI1KGh? NF;sO5lFVVOR NETJOW1qdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? M4rNZVI1Ojl5M{Cw
Huh7  NGPPVllHfW6ldHnvckBCe3OjeR?= M{C5blExOCCwTR?= NV7vcppWOTJiaB?= M{DvV2ROW09? NGHONYpqdmS3Y3XzJIF2fG:yaHHnfeKh MnnLNlQzQTd|MEC=
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TE671 MX\BdI9xfG:|aYOgRZN{[Xl? MmHoNUDPxE1? MnTJOFghcA>? M4K0SYlv[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JGFDXC15M{e= MnrHNlQyOzN{MUi=
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Hep-2 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{S5bFAvODFvMTFOwG0> Ml\GNVIuQTZiaB?= NXu3eppLcW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ MnLMNlQyODN5NEm=
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Hep-2 NYHrRnlwTnWwY4Tpc44hSXO|YYm= NGHKXW0xNjFxMD61JO69VQ>? MUmyOEBp Ml;SZoxw[2u|IH3UU3JEOSCjbnSgcXRQWkN{IHHjeIl3[XSrb36= Mn7jNlQyODN5NEm=
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HT-29 MWLBdI9xfG:|aYOgRZN{[Xl? M1rlZVEh|ryP NFK4[3Q4OiCq M2e5PIF1fGWwdXH0[ZMhPS2IVT3pcoR2[2WmIHPlcIwh\GWjdHi= M{TwWVIyQTR7MUKx
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HT-29 MWfGeY5kfGmxbjDBd5NigQ>? MVqxJO69VQ>? NH[3NlY4OiCq NXnveGUzcW6mdXPld{BifXSxcHjh[5khfG9iY3;u[oVzKGO7dH;wdo91\WO2aX;u MYGyNVk1QTF{MR?=
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Rh41 NYLNO2FUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTNyLkegcm0> NWLFbFBFOjF|M{e2O|k>
Rh18 Mn\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3O3VWlEPTB;NEGuOEBvVQ>? M3\xNFIyOzN5Nke5
Rh30 M2r4bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvGTWM2OD1zNDDuUS=> MorqNlE{Ozd4N{m=
BT-12 MlHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTJ2Lkegcm0> NHnLVlgzOTN|N{[3PS=>
CHLA-266 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLkS|JQUUN3ME2xMlEhdk1? M33nZlIyOzN5Nke5
TC-71 NUO2TWRDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITLS5dKSzVyPUS2MlUhdk1? NHLLR3AzOTN|N{[3PS=>
CHLA-9 NH;ZbYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGO5OHlKSzVyPUG1MlMhdk1? MXGyNVM{PzZ5OR?=
CHLA-10 NFXsW3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPiR4tmUUN3ME2zMlkhdk1? MV2yNVM{PzZ5OR?=
CHLA-258 NWPMc|NJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LnNmlEPTB;MUCuOEBvVQ>? MWSyNVM{PzZ5OR?=
GBM2 Mn3SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTRyLkegcm0> NHv2b4IzOTN|N{[3PS=>
NB-1643 MkPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;kVphPUUN3ME2zN{45KG6P MoPlNlE{Ozd4N{m=
NB-EBc1 NWDJSGJ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\2cmlEPTB;MkeuNUBvVQ>? M3rGblIyOzN5Nke5
CHLA-90 MlvkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1izcWlEPTB;M{SuOUBvVQ>? Ml35NlE{Ozd4N{m=
CHLA-136 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;iPJBKSzVyPUG2Nk43KG6P M1K3bVIyOzN5Nke5
NALM-6 Mm\oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33KfGlEPTB;M{KuPUBvVQ>? NHvVc48zOTN|N{[3PS=>
COG-LL-317 NUfSUJB6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTFwMzDuUS=> M3jIVlIyOzN5Nke5
RS4;11 NGrae3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvvSmtKSzVyPUO1JI5O NVezWIxvOjF|M{e2O|k>
MOLT-4 MlGyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojpTWM2OD16Lk[gcm0> Mke1NlE{Ozd4N{m=
CCRF-CEM NVHlXlByT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTRwMTDuUS=> NWf4emFLOjF|M{e2O|k>
Kasumi-1 NVvYOHFjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTFwNDDuUS=> M2HvNVIyOzN5Nke5
Karpas-299 Mk\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGftWYtKSzVyPUKxMlchdk1? M1nkN|IyOzN5Nke5
Ramos-RA1 NViwNoZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\tN25yUUN3ME24MlIhdk1? MVWyNVM{PzZ5OR?=
H838 M{jLWWZ2dmO2aX;uJGF{e2G7 M1LjO|Eh|ryP M3PpVFQ5KGh? NITvc2hqdmS3Y3XzJINmdGxiYYX0c5Bp[We7 NFW5d4MzODN4NEGxNy=>
H838 Mme3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXWwMVMh|ryP NYXuUFZxPzJiaB?= MWfpcoR2[2W|IHfyc5d1cCCrbnjpZol1cW:wIHHu[EBifXSxcHjh[5k> MWOyNFAzQDh3NB?=
A549  M3yzOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXGVFUxNTNizszN M3vZWVczKGh? NGfRWYhqdmS3Y3XzJIdzd3e2aDDpcohq[mm2aX;uJIFv\CCjdYTvdIhi\3l? MlT2NlAxOjh6NUS=

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID