AZD8055

For research use only.

Catalog No.S1555

167 publications

AZD8055 Chemical Structure

CAS No. 1009298-09-2

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.

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Selleck's AZD8055 has been cited by 167 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HlUWlEPTB;MD6wNlM{OSEQvF2= NXfiRo5TW0GQR1XS
NCI-H720 NGnURVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV[xOWdLUUN3ME2wMlAzQTN6IN88US=> MWDTRW5ITVJ?
KARPAS-45 M2Tlcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXsTWM2OD1yLkCzN|cyKM7:TR?= MX7TRW5ITVJ?
EW-3 NGnGOm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\kTWM2OD1yLkC0NFU3KM7:TR?= M{X2ZnNCVkeHUh?=
SBC-1 NWm4NmpwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jDO2lEPTB;MD6wOFU5OSEQvF2= Ml64V2FPT0WU
NCI-H209 NUDlNmZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7Wc2hKSzVyPUCuNFU1PTNizszN MVzTRW5ITVJ?
EoL-1-cell MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTBwME[1Olkh|ryP MnnlV2FPT0WU
H9 NEnHfplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fJRmlEPTB;MD6wOlY6OiEQvF2= NXLpNFVrW0GQR1XS
AN3-CA M{\wXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXtTWM2OD1yLkC3PVUyKM7:TR?= NUDqc5E6W0GQR1XS
HH MlXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2L6fWlEPTB;MD6wPFAxQSEQvF2= MlGzV2FPT0WU
KU812 MmnaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnPUppKSzVyPUCuNFgxPDNizszN M4DuNnNCVkeHUh?=
DU-4475 NYHDV2hnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTBwMEiwOFch|ryP NWj4Z2sxW0GQR1XS
CTV-1 NYfjcGxIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3zwemlEPTB;MD6wPFY1OSEQvF2= MU\TRW5ITVJ?
BV-173 MnT0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTBwMEmwOlEh|ryP MkKxV2FPT0WU
ES5 Mn;vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDNW5I5UUN3ME2wMlA6OzVizszN MkjEV2FPT0WU
G-402 M1fhN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PaemlEPTB;MD6wPVk5KM7:TR?= M{T6[HNCVkeHUh?=
MV-4-11 M{ftPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzGTVZKSzVyPUCuNVA{PDdizszN MUnTRW5ITVJ?
NB69 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTOUndOUUN3ME2wMlE{PSEQvF2= NUH2VVhEW0GQR1XS
EGI-1 MlL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HmPWlEPTB;MD6xN|U{OSEQvF2= M3rWcHNCVkeHUh?=
EW-18 M4i5fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTBwMUSwO|Mh|ryP MkjvV2FPT0WU
ME-180 NWDJUGQ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXmTWM2OD1yLkG0OlgyKM7:TR?= NFjGbYlUSU6JRWK=
D-283MED M3fiPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTBwMUS3NlMh|ryP NYrmTWtVW0GQR1XS
NB14 MorZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnwSJFKSzVyPUCuNVY1PDdizszN MVXTRW5ITVJ?
Ramos-2G6-4C10 NETBfJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTXTWM2OD1yLkG2PVg{KM7:TR?= NHO4ZmZUSU6JRWK=
NBsusSR NUC0VoF1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7lW3A{UUN3ME2wMlE4ODF7IN88US=> MkHDV2FPT0WU
LAMA-84 NHzBXpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HpbmlEPTB;MD6xPFA{OiEQvF2= MVnTRW5ITVJ?
NCI-H1581 MmjOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTBwMUi5OlYh|ryP M1HYNHNCVkeHUh?=
A204 MlvUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvtTWM2OD1yLkG4PVch|ryP NGjoT3pUSU6JRWK=
A549 NX;HT202T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYe1NXU2UUN3ME2wMlE6PTVizszN M2XNU3NCVkeHUh?=
CAL-51 M{LLPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnkTWM2OD1yLkG5OlUyKM7:TR?= NVXEfnM3W0GQR1XS
NKM-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mni5TWM2OD1yLkKwNFI6KM7:TR?= M4O4SnNCVkeHUh?=
LoVo MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUL2d2E1UUN3ME2wMlIxOjB{IN88US=> MXjTRW5ITVJ?
HAL-01 NVXERnF4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIftTHNKSzVyPUCuNlAzQTdizszN MmHuV2FPT0WU
HCC2218 Mnf0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnKOWI{UUN3ME2wMlIxPjh6IN88US=> MWPTRW5ITVJ?
BEN MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rxZmlEPTB;MD6yNVI6OyEQvF2= NGfKflhUSU6JRWK=
LC-2-ad M3K2W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTBwMkKwPFQh|ryP NV:y[4JzW0GQR1XS
LXF-289 NH33e2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LHS2lEPTB;MD6yOVA1OSEQvF2= NYfLPZdDW0GQR1XS
HGC-27 NWjKXnM5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTyTWM2OD1yLkK2OFE1KM7:TR?= NXfq[ZVnW0GQR1XS
LK-2 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnK5TWM2OD1yLkK2PFI1KM7:TR?= MY\TRW5ITVJ?
CTB-1 MlfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTBwMkeyNVUh|ryP MmLVV2FPT0WU
697 NGjud3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTBwMkezOlch|ryP NF\kSIpUSU6JRWK=
ES7 NFLTdXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\GUZRKSzVyPUCuNlc{QDNizszN MkTFV2FPT0WU
MG-63 NVPNXmdnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3KTWM2OD1yLkK3OFA4KM7:TR?= NH;xWVBUSU6JRWK=
T47D MmHLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{ToUmlEPTB;MD6yO|Q4PSEQvF2= NGj3SZpUSU6JRWK=
RPMI-8226 NUXhfFR4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rGZ2lEPTB;MD6yPFU5QSEQvF2= NGL5[VZUSU6JRWK=
NCI-H292 NIW4UGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;hVJd6UUN3ME2wMlI6OTZ2IN88US=> NUnRU5VqW0GQR1XS
HT M37PN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rGfGlEPTB;MD6yPVM5KM7:TR?= MWHTRW5ITVJ?
A3-KAW Ml;DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLRTWM2OD1yLkK5O|Qh|ryP M3;SdnNCVkeHUh?=
LB2241-RCC NXPTNGx7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2T4[GlEPTB;MD6zNFE6QCEQvF2= MkjQV2FPT0WU
BB65-RCC NEK2[Y5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnsTWM2OD1yLkOwPFA2KM7:TR?= MnPlV2FPT0WU
G-401 NIT0UXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1OzfGlEPTB;MD6zNFk5PiEQvF2= NV;zfnZnW0GQR1XS
TYK-nu MkT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTBwM{G4OlQh|ryP NG\oWmdUSU6JRWK=
NCI-SNU-1 NWXrNYs3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PJT2lEPTB;MD6zNlM{OSEQvF2= NGP4cnBUSU6JRWK=
CHL-1 MoH5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkT1TWM2OD1yLkOyO|A{KM7:TR?= M4HDUnNCVkeHUh?=
P30-OHK NVjjOG95T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\YSm54UUN3ME2wMlMzPzB3IN88US=> NEPaUIlUSU6JRWK=
PA-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDPTWM2OD1yLkOzOlE6KM7:TR?= MYTTRW5ITVJ?
LB1047-RCC NWPyWIZzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4rMb2lEPTB;MD6zOFA1PyEQvF2= MUHTRW5ITVJ?
EM-2 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYPqWVh3UUN3ME2wMlM1OTh6IN88US=> NGDZSIFUSU6JRWK=
VA-ES-BJ NInROmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkPGTWM2OD1yLkO0NlY5KM7:TR?= MV\TRW5ITVJ?
NCI-H520 MmixS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\LTWM2OD1yLkO0PFM1KM7:TR?= MXvTRW5ITVJ?
ECC10 NWfZUWwyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TCOWlEPTB;MD6zOVA{PSEQvF2= MUDTRW5ITVJ?
LNCaP-Clone-FGC NULlR4dGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjRXY5KSzVyPUCuN|U{OTRizszN Mn60V2FPT0WU
ES8 NUDLZpFqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;VTWM2OD1yLkO2OVU{KM7:TR?= NIHye4RUSU6JRWK=
HuO-3N1 NX7WXJZCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTnTWM2OD1yLkO4N|c6KM7:TR?= NETZPIZUSU6JRWK=
L-363 NUDqW|d2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\nTWM2OD1yLkO5NFI3KM7:TR?= M4rLZXNCVkeHUh?=
SK-LU-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPQTWM2OD1yLkO5NlA5KM7:TR?= MXrTRW5ITVJ?
MCF7 NVPVXFJoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17WVmlEPTB;MD60NFU{KM7:TR?= NF3KU21USU6JRWK=
HuO9 NIjUSItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzFTWM2OD1yLkSwOlY4KM7:TR?= MYfTRW5ITVJ?
BHT-101 NIHkdYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzwfpFKSzVyPUCuOFA4QDdizszN M2jjfXNCVkeHUh?=
MZ2-MEL NF3Ne|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVz6WXhkUUN3ME2wMlQxQDh{IN88US=> MVvTRW5ITVJ?
NOS-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH[4N2dKSzVyPUCuOFExPzRizszN MoHKV2FPT0WU
NEC8 NIDRTolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;xcJoyUUN3ME2wMlQyPjl3IN88US=> MofsV2FPT0WU
COR-L88 M4XJfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XqTmlEPTB;MD60NlI3PyEQvF2= NVTKWlNzW0GQR1XS
MFM-223 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvMcXBuUUN3ME2wMlQzPjF5IN88US=> MljmV2FPT0WU
HSC-3 NEDoTmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTBwNEK5NFgh|ryP MVPTRW5ITVJ?
OVCAR-5 NHL4clRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFe0VXlKSzVyPUCuOFI6OzNizszN MVjTRW5ITVJ?
MFE-280 MkS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPpTWM2OD1yLkSzNFMzKM7:TR?= M3vpeHNCVkeHUh?=
KP-N-YS NIfQbWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXQTWM2OD1yLkSzNFch|ryP M4LzN3NCVkeHUh?=
EPLC-272H MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUG3c2RtUUN3ME2wMlQ{ODd5IN88US=> MnzUV2FPT0WU
KE-37 NEfuVolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fOWGlEPTB;MD60N|I1PyEQvF2= NEfIUpZUSU6JRWK=
HuH-7 M1zXeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWW4O5hUUUN3ME2wMlQ{Ozd{IN88US=> NGHxXo1USU6JRWK=
CHP-212 NX;6NpA{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHzWVdKSzVyPUCuOFM3QTdizszN MmfBV2FPT0WU
SK-MES-1 NX;tNXNXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvBTpBrUUN3ME2wMlQ{QDR6IN88US=> NXvSSYp1W0GQR1XS
SJSA-1 MoHxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDRcWJ[UUN3ME2wMlQ1PTV2IN88US=> NYnPTFBTW0GQR1XS
QIMR-WIL MorUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTBwNEWxOUDPxE1? MYPTRW5ITVJ?
ES4 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTBwNEWzN|Yh|ryP M4fjVHNCVkeHUh?=
TI-73 NWqyUmtIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPTcmVKSzVyPUCuOFU3QDZizszN M2PrenNCVkeHUh?=
COLO-684 NWrHXndkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PPSGlEPTB;MD60OVczPiEQvF2= Mn3zV2FPT0WU
GCIY M2\Se2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvzOXljUUN3ME2wMlQ2PzV2IN88US=> MYLTRW5ITVJ?
JVM-3 MonDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTBwNE[yPVIh|ryP NGfLfXRUSU6JRWK=
MPP-89 Ml7FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPaTWM2OD1yLkS2PVQ4KM7:TR?= M1r5[XNCVkeHUh?=
M14 MmnhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PpTWlEPTB;MD60O|E4KM7:TR?= NX;uWI9yW0GQR1XS
SW962 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTBwNEexPUDPxE1? NEH3N45USU6JRWK=
VM-CUB-1 MnjHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\mUWlEPTB;MD60O|I5PiEQvF2= M{H4VXNCVkeHUh?=
A101D MlS3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoX0TWM2OD1yLkS4PVY5KM7:TR?= Mnr3V2FPT0WU
DB NEm4cotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\OWGlEPTB;MD61NFAzOSEQvF2= NXfSWIN5W0GQR1XS
KNS-62 MnLHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTBwNUC1Olgh|ryP MYXTRW5ITVJ?
NCI-H460 MlXnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnjd4VKSzVyPUCuOVA2QTdizszN MV\TRW5ITVJ?
MDA-MB-361 NF\MboZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXTTWM2OD1yLkWwPFch|ryP NEnxT29USU6JRWK=
DOK MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDPbI1bUUN3ME2wMlUyOzN|IN88US=> Ml\oV2FPT0WU
CAL-39 NU\zNpFQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTBwNUKyOlgh|ryP M1zIWHNCVkeHUh?=
HT-1080 M3X3eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTBwNUK1OFMh|ryP NH;aWZVUSU6JRWK=
GR-ST NUHPRll7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jMdmlEPTB;MD61N|AyPSEQvF2= M3LRfnNCVkeHUh?=
J-RT3-T3-5 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTBwNUOxPVUh|ryP MnTaV2FPT0WU
NCI-H1793 NVjZPIFDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX6yUGVMUUN3ME2wMlU{PDR5IN88US=> Ml[zV2FPT0WU
NCI-H1355 M2X2Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILwfVZKSzVyPUCuOVM5PzhizszN NWHnZ3ZZW0GQR1XS
YH-13 M{n0OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTBwNUS5N|gh|ryP MnPxV2FPT0WU
LOXIMVI Mm\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3m2WGlEPTB;MD61OVEzOyEQvF2= NYnPfZF[W0GQR1XS
ES1 Mlu3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DKSWlEPTB;MD61OVUyQCEQvF2= NWTvVIs4W0GQR1XS
GAMG MorDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\Ke2lEPTB;MD61OVY2QCEQvF2= M4rLVXNCVkeHUh?=
SK-MEL-28 M3Xlbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUf5[Zg3UUN3ME2wMlU2QTFizszN MmLhV2FPT0WU
KG-1 NUL6SFlGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXvsPVEzUUN3ME2wMlU3QDh2IN88US=> NXfiPWszW0GQR1XS
HOS MlzTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTBwNUexPVIh|ryP MXnTRW5ITVJ?
PANC-08-13 M3LRdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\sb4ZKSzVyPUCuOVc{QDlizszN MWLTRW5ITVJ?
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SW684 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHy1SZRKSzVyPUGuPVc{PzFizszN M3jM[nNCVkeHUh?=
NCI-H1693 NILL[25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rxeGlEPTB;MT65PFA5PiEQvF2= M3LaZnNCVkeHUh?=
8-MG-BA MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWToZ21vUUN3ME2xMlk5OjFizszN MVTTRW5ITVJ?
OVCAR-8 NVHrS3pqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTJwMECyPVUh|ryP NH7qW5RUSU6JRWK=
DSH1 NXrx[nkxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrqSGNKSzVyPUKuNFE4PzRizszN M2[y[3NCVkeHUh?=
KYSE-520 M1jJW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnoOYg5UUN3ME2yMlAzODR7IN88US=> MoDzV2FPT0WU
GB-1 NWn3SHlKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jRZWlEPTB;Mj6wNlU4PyEQvF2= M1H6RnNCVkeHUh?=
RCC10RGB Ml7KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;Xd2V6UUN3ME2yMlA1QDdzIN88US=> NEXueWpUSU6JRWK=
SW954 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3uxbGlEPTB;Mj6wOVA{PiEQvF2= MmrjV2FPT0WU
IST-MES1 M{fTcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnpNXhUUUN3ME2yMlA2PTZ6IN88US=> NVXWZ3Q5W0GQR1XS
AU565 NEnUcnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTWTWM2OD1{LkC1PVA2KM7:TR?= MXjTRW5ITVJ?
CW-2 NE\LZm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTJwME[4O|Uh|ryP NFvOXlJUSU6JRWK=
TE-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYK0c2pZUUN3ME2yMlA6Ozd5IN88US=> NHP5ZWxUSU6JRWK=
KP-4 MmDqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLITWM2OD1{LkGwN|EzKM7:TR?= NGHKfGdUSU6JRWK=
SNU-C2B NYnJNXpQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{i2cmlEPTB;Mj6xNFgzOyEQvF2= NH:5bIhUSU6JRWK=
NCI-H1395 MonPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTzTWM2OD1{LkGwPVg3KM7:TR?= MUjTRW5ITVJ?
CAS-1 NG\mOppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTJwMUG1OFYh|ryP MV3TRW5ITVJ?
NCI-H1155 NF31U21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlj0TWM2OD1{LkKyOlkh|ryP M{TGdHNCVkeHUh?=
SK-MEL-1 NVP5So15T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnKdoV5UUN3ME2yMlI5OThzIN88US=> NVT1ZlhZW0GQR1XS
KINGS-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTJwM{CwPFgh|ryP NIjRZndUSU6JRWK=
ESS-1 NUnhSVFzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTJwM{C1NUDPxE1? M4DqeXNCVkeHUh?=
PANC-10-05 NF7WdG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDXTWM2OD1{LkO3NFI1KM7:TR?= NYjhfI9pW0GQR1XS
HT-1376 NUOySmxzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XmfmlEPTB;Mj6zPFcxQCEQvF2= M3nPe3NCVkeHUh?=
LS-513 NXTnSYp[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYL3UIxVUUN3ME2yMlM5QDd2IN88US=> NFjkOZlUSU6JRWK=
KNS-42 M3HWVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHzOXZWUUN3ME2yMlM6OTB3IN88US=> MYXTRW5ITVJ?
639-V Ml;yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzzTY9KSzVyPUKuOFI4PThizszN MVfTRW5ITVJ?
SJRH30 MojuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEL3VmdKSzVyPUKuOFUyPjFizszN M{LEcnNCVkeHUh?=
SW1783 M2jYdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHG5OoZKSzVyPUKuOFcxPzhizszN NYPFOmFYW0GQR1XS
NB6 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\MbmlEPTB;Mj60PFI5KM7:TR?= MYjTRW5ITVJ?
SK-MEL-30 M2jhd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nGUWlEPTB;Mj60PFg{KM7:TR?= NGHCSFJUSU6JRWK=
HCE-4 M3jQT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIjhT4NKSzVyPUKuOFk1ODJizszN NYP4b5hWW0GQR1XS
NB10 NHXQbJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYC0fZFjUUN3ME2yMlQ6PDJ7IN88US=> NFnpTWNUSU6JRWK=
H-EMC-SS NXq5N|V3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrMTWM2OD1{LkW0OlUh|ryP NInSfFVUSU6JRWK=
SK-HEP-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTJwNUWyN|Yh|ryP NEmzSmhUSU6JRWK=
HCC1395 M3HMV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzyeGRKSzVyPUKuOVU3PTVizszN M4jyRXNCVkeHUh?=
HuCCT1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jNSWlEPTB;Mj61OlM6KM7:TR?= NU\Y[GNrW0GQR1XS
SiHa MmXaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\PR2lEPTB;Mj61PFcyQSEQvF2= M2fueXNCVkeHUh?=
EFO-21 M2nvOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXLVW1KSzVyPUKuOVg4PjRizszN NGfqTldUSU6JRWK=
NCI-H1666 NFPGW4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDjTWM2OD1{LkW5NFc3KM7:TR?= NU\BXolkW0GQR1XS
UMC-11 M3nJR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrCTWM2OD1{LkW5PFY5KM7:TR?= M4DGVnNCVkeHUh?=
COLO-679 M1L0e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnWSY5yUUN3ME2yMlYyOjN|IN88US=> NGS2WZVUSU6JRWK=
A388 MkfiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjk[|JKSzVyPUKuOlUzPTNizszN MkiwV2FPT0WU
LB2518-MEL NH;CZWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTJwNkW0NFgh|ryP MV;TRW5ITVJ?
EW-13 M1izWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjhb21KSzVyPUKuOlg4PjhizszN NXrmXXlFW0GQR1XS
MLMA MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHQ[IR5UUN3ME2yMlY5QDV4IN88US=> MWfTRW5ITVJ?
HOP-92 NUX4VoZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTJwN{CxNUDPxE1? NXPmb3RHW0GQR1XS
NOMO-1 M3[xe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPLWodxUUN3ME2yMlcyPDR{IN88US=> M2j0OHNCVkeHUh?=
NCI-H810 MlznS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XTNWlEPTB;Mj63NlE6QCEQvF2= NUHDc5RzW0GQR1XS
769-P NYDHTYdCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXvLfllJUUN3ME2yMlczOjhizszN MWnTRW5ITVJ?
SAS MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXiwbGV3UUN3ME2yMlc3PSEQvF2= NUPjVXdGW0GQR1XS
M059J NWrRe21KT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NILQbotKSzVyPUKuO|g{OThizszN NXPwTmVkW0GQR1XS
RD NFrMZ4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3XyW2lEPTB;Mj63PVQ4OSEQvF2= MXHTRW5ITVJ?
GT3TKB Mof1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTJwOEO0N|Mh|ryP M{i2bnNCVkeHUh?=
MZ7-mel MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTJwOE[1NFIh|ryP M4LGbHNCVkeHUh?=
KYSE-510 NFfn[IxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTJwOEm1NFQh|ryP NVzyT45UW0GQR1XS
NCI-N87 NGXCOW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7zTWM2OD1{LkmxPFA4KM7:TR?= M1;VW3NCVkeHUh?=
MKN1 MlfWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3qXohKSzVyPUOuNFAyPjNizszN NUPuZ4JPW0GQR1XS
NCI-H2170 NInWWHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{j3dWlEPTB;Mz6wNFQ2OSEQvF2= MofEV2FPT0WU
OVCAR-3 NXzITmtvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjwPJpKSzVyPUOuNFIyOTVizszN NGjhUXVUSU6JRWK=
KLE M1y0bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjpTWM2OD1|LkC2PFEzKM7:TR?= NEDwS|VUSU6JRWK=
LB771-HNC NEOwTWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmGyTWM2OD1|LkC5N|Q{KM7:TR?= MmfkV2FPT0WU
MC116 NGnI[|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzsN5lKSzVyPUOuNVEyOTJizszN NHuxR2xUSU6JRWK=
SCC-9 M2Tle2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37sW2lEPTB;Mz6xNVM3PiEQvF2= NVnRPHB5W0GQR1XS
U031 MoLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXmR2tZUUN3ME2zMlEzPDJ|IN88US=> NXvFbpFPW0GQR1XS
LCLC-103H NYf3RVFxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoS1TWM2OD1|LkKzPFk1KM7:TR?= M4LDSXNCVkeHUh?=
U-266 Mk\HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7vW|hKSzVyPUOuNlc3OzNizszN NWO1WnZJW0GQR1XS
JVM-2 NF;yUHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoftTWM2OD1|LkOyNVY1KM7:TR?= NV3HdpRDW0GQR1XS
UACC-257 Ml7zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTNwNE[0N|Eh|ryP MmrkV2FPT0WU
LB996-RCC M{HER2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDOPVdKSzVyPUOuOFY2ODJizszN NWLOcohHW0GQR1XS
BT-549 NEXNRpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLxTWM2OD1|LkWwNlA6KM7:TR?= MV\TRW5ITVJ?
NCI-H526 M4\wT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\hc25KSzVyPUOuOVQyPThizszN MoDnV2FPT0WU
RH-18 NELmd|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\xTWM2OD1|Lk[xO|k5KM7:TR?= NUnsT21TW0GQR1XS
SW626 Ml7US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4W4fmlEPTB;Mz63NVEzQCEQvF2= NUXNdnhmW0GQR1XS
SK-N-AS MkDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGe1OIFKSzVyPUOuO|MzPDdizszN MW\TRW5ITVJ?
IGR-1 NG\pRXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1Tk[2lEPTB;Mz63OFI1QCEQvF2= M330WnNCVkeHUh?=
NCI-H2291 M2PBSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnvOoE5UUN3ME2zMlc2OzN4IN88US=> M1TsNnNCVkeHUh?=
NCI-H441 NYiyZoZoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlrTTWM2OD1|LkiyOFM{KM7:TR?= NUH0dXVjW0GQR1XS
D-247MG MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTNwOE[wN|Eh|ryP NXj6dYZUW0GQR1XS
COLO-320-HSR Ml7PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHTPVlIUUN3ME2zMlkyOjdzIN88US=> MofHV2FPT0WU
A253 M3jJ[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFXOWXZKSzVyPUOuPVE1OzZizszN MVjTRW5ITVJ?
HT55 NIm4W5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTNwOUG3O|Mh|ryP NIXYd2xUSU6JRWK=
MZ1-PC MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnoTlFQUUN3ME2zMlk2QDZ2IN88US=> MlHIV2FPT0WU
RERF-LC-MS MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrYdJlKSzVyPUOuPVY2OjVizszN MU\TRW5ITVJ?
SF268 NE\4PHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjufJRKSzVyPUSuNVI5QDFizszN MY\TRW5ITVJ?
RMG-I NXnaSJBWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjXTWM2OD12LkKxOFU1KM7:TR?= M4i2WnNCVkeHUh?=
CFPAC-1 MkLTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnz2TWM2OD12LkKxOlg2KM7:TR?= M4LoXnNCVkeHUh?=
S-117 NWW0TIVvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;2eWlEPTB;ND6yNlAzPCEQvF2= NEXNcXhUSU6JRWK=
NCI-H2122 NYDDZYhqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHuXmJnUUN3ME20MlMxQTNzIN88US=> MVLTRW5ITVJ?
J82 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELGS5BKSzVyPUSuN|M1PDFizszN MXrTRW5ITVJ?
CAL-72 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTmeXhKSzVyPUSuN|U1PzdizszN MVjTRW5ITVJ?
GP5d MkLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTRwNEGyPVgh|ryP NWPVd3ZsW0GQR1XS
UACC-62 M3rYbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3KzcmlEPTB;ND60OlU1PSEQvF2= NYLMNlR6W0GQR1XS
MS-1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2X6OmlEPTB;ND60PVEyPSEQvF2= NV3IVYNOW0GQR1XS
RCM-1 M1fOcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;0TWM2OD12LkWyPVIh|ryP Mn7OV2FPT0WU
IST-SL1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXTWGlKSzVyPUSuOlU{PTlizszN MVzTRW5ITVJ?
SW756 MoSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33s[GlEPTB;ND62PVE{PSEQvF2= NFfZWWFUSU6JRWK=
HCC38 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTRwN{GxPVch|ryP Ml;2V2FPT0WU
YAPC M2\4fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3Tdmt5UUN3ME20MlcyOzF|IN88US=> MX\TRW5ITVJ?
DK-MG M1jTcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkKzTWM2OD12Lke4NVEzKM7:TR?= Mlu0V2FPT0WU
HCC1937 M{n0dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\JenVLUUN3ME20Mlg2OTJ3IN88US=> MXvTRW5ITVJ?
OAW-28 NWDYW2JIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\2TWM2OD12LkmyOFM2KM7:TR?= MkjsV2FPT0WU
MEG-01 Mn3TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHkTWM2OD13LkC4N|I5KM7:TR?= M1\jb3NCVkeHUh?=
GI-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTVwMkO0PFYh|ryP NUTnUWZbW0GQR1XS
FADU NGPWTGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTVwMke4N|ch|ryP M4jUeHNCVkeHUh?=
CAPAN-1 NUXnV29XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjtcHVbUUN3ME21MlM6PDN{IN88US=> MnrNV2FPT0WU
TGBC11TKB M1vHUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLuTWM2OD13LkS1O|g3KM7:TR?= M4fkWnNCVkeHUh?=
DBTRG-05MG Mo\wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUG1fYdPUUN3ME21MlUxPTJizszN NUPZblZPW0GQR1XS
ONS-76 NYH2W2dHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfiTWM2OD13LkW1PFAyKM7:TR?= NFm1Um1USU6JRWK=
Ca-Ski NVK1foZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI[xWYRKSzVyPUWuOlI2PjZizszN NWnkNo91W0GQR1XS
COLO-741 M4K3N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zHdmlEPTB;NT63NVk6PSEQvF2= NIrrNY1USU6JRWK=
HT-29 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;MT3FKSzVyPUWuPFA1PzVizszN NEDReIJUSU6JRWK=
HCC1187 M3G0R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTVwOEGwNFgh|ryP MnPzV2FPT0WU
Capan-2 M1LRdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jSUGlEPTB;NT65OVk6PCEQvF2= NXTMPG5HW0GQR1XS
NCI-H630 M1jNSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHOenZKSzVyPU[uNVE1PDFizszN M4fGbXNCVkeHUh?=
COLO-678 NEHMRWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPwPZpKSzVyPU[uNVUxQTJizszN NXvjbFhzW0GQR1XS
D-502MG MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofjTWM2OD14LkG3N|M3KM7:TR?= MnLOV2FPT0WU
NB17 M{\rSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTZwMkS5OFMh|ryP NX\BdIZJW0GQR1XS
SK-MEL-24 NFnLWXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfnTWM2OD14LkK2OFkh|ryP MV;TRW5ITVJ?
NCI-SNU-5 NHruS2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{n4[mlEPTB;Nj6yO|EyQCEQvF2= MnqxV2FPT0WU
D-392MG NX\sRYkxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUmybG1{UUN3ME22MlM{Ozl7IN88US=> NU\yb3R6W0GQR1XS
A498 M1Lo[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2PFe2lEPTB;Nj60NFMxOiEQvF2= NEKwbVhUSU6JRWK=
OCI-AML2 M1H1e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLTeo9KUUN3ME22MlQ2PDR3IN88US=> NFXVVnlUSU6JRWK=
HCC2157 M3\Wc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVG5cnVtUUN3ME22MlQ2PjF4IN88US=> NUjmeG95W0GQR1XS
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COG-LL-317 M2LUWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfTNoRKSzVyPUGuN{BvVQ>? M4DFNVIyOzN5Nke5
RS4;11 NU\4bGxGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWS3XnZTUUN3ME2zOUBvVQ>? NHLIb|MzOTN|N{[3PS=>
MOLT-4 MlvoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHROWZKSzVyPUiuOkBvVQ>? MXSyNVM{PzZ5OR?=
CCRF-CEM NXXVb4x5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfuTWM2OD12LkGgcm0> M1u5[FIyOzN5Nke5
Kasumi-1 NGTqfnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvvdnlKSzVyPUGuOEBvVQ>? NVvrV4ZnOjF|M{e2O|k>
Karpas-299 NYHIZ4RWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGftNW9KSzVyPUKxMlchdk1? NFLhXVUzOTN|N{[3PS=>
Ramos-RA1 NGPWOlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rFSmlEPTB;OD6yJI5O NFjCdHgzOTN|N{[3PS=>
H838 NVviW5VxTnWwY4Tpc44hSXO|YYm= NF6zNFgyKM7:TR?= NXj4PXFnPDhiaB?= NGrib5RqdmS3Y3XzJINmdGxiYYX0c5Bp[We7 MlXlNlA{PjRzMUO=
H838 NWLMcppsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnLdlA4OC1|IN88US=> MVu3NkBp NGrDe5FqdmS3Y3XzJIdzd3e2aDDpcohq[mm2aX;uJIFv\CCjdYTvdIhi\3l? M3v6c|IxODJ6OEW0
A549  MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX6wMVMh|ryP M335flczKGh? M1vDbIlv\HWlZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKGG3dH;wbIFogQ>? NF7vcYQzODB{OEi1OC=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; 

PubMed: 24457069     


Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙

p-EGFR / EGFR; 

PubMed: 25654224     


PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot;

p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; 

PubMed: 25654224     


PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.

24457069 25654224
Immunofluorescence
p-4EBP1 / EIF4E / mTOR; 

PubMed: 24600487     


(C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋€𢡄�෋€䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemセ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒セ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wセ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v 玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴￿￿嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗

LC3B / p62 ; 

PubMed: 30337536     


Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞

24600487 30337536
Growth inhibition assay
Cell viability ; 

PubMed: 29263324     


Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.

29263324
In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
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Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
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  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Water Insoluble
Ethanol '3 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A
Smiles CC1COCCN1C2=NC(=NC3=C2C=CC(=N3)C4=CC(=C(C=C4)OC)CO)N5CCOCC5C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

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Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID