AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 40 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

  • HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

    Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017, 15(1):9. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 NEjrVplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXxd4lQUUN3ME2wMlAzOzNzIN88US=> M{PRVHNCVkeHUh?=
NCI-H720 M4LYdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTRVnVKSzVyPUCuNFI6OzhizszN MYjTRW5ITVJ?
KARPAS-45 NHXTdoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTBwMEOzO|Eh|ryP M364eXNCVkeHUh?=
EW-3 M{S1WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTBwMESwOVYh|ryP MnSxV2FPT0WU
SBC-1 NEjGbGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWizV2pJUUN3ME2wMlA1PThzIN88US=> NUezPWF6W0GQR1XS
NCI-H209 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\sTGlEPTB;MD6wOVQ2OyEQvF2= NULi[4lIW0GQR1XS
EoL-1-cell NVvVW4NJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjCc5pUUUN3ME2wMlA3PTZ7IN88US=> NHLXV4hUSU6JRWK=
H9 M{\BTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTBwME[2PVIh|ryP MnLrV2FPT0WU
AN3-CA MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3K2cmlEPTB;MD6wO|k2OSEQvF2= MnHjV2FPT0WU
HH MnztS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXO0fnBMUUN3ME2wMlA5ODB7IN88US=> MlPNV2FPT0WU
KU812 NYjwOVVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PVe2lEPTB;MD6wPFA1OyEQvF2= MV\TRW5ITVJ?
DU-4475 NWjuOoVqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3DdGFKSzVyPUCuNFgxPDdizszN NFPN[YtUSU6JRWK=
CTV-1 NH3DU5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHRTWM2OD1yLkC4OlQyKM7:TR?= NVvJUnhqW0GQR1XS
BV-173 NFnoUWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfROVFzUUN3ME2wMlA6ODZzIN88US=> NYT3TlZ3W0GQR1XS
ES5 NFHheVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH71V2pKSzVyPUCuNFk{PSEQvF2= M4HHOnNCVkeHUh?=
G-402 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTBwMEm5PEDPxE1? MXjTRW5ITVJ?
MV-4-11 MoHLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;VTWM2OD1yLkGwN|Q4KM7:TR?= NIfsPY5USU6JRWK=
NB69 Ml7RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4THTGlEPTB;MD6xN|Uh|ryP NGTFTFBUSU6JRWK=
EGI-1 NWj3RYRWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVTIZphEUUN3ME2wMlE{PTNzIN88US=> M4fU[3NCVkeHUh?=
EW-18 NVfmR|ZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmK5TWM2OD1yLkG0NFc{KM7:TR?= M4L2OXNCVkeHUh?=
ME-180 NHzHbFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHBWFRiUUN3ME2wMlE1PjhzIN88US=> MU\TRW5ITVJ?
D-283MED NFfsW|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrLRnhKSzVyPUCuNVQ4OjNizszN NILrcHJUSU6JRWK=
NB14 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITwW3dKSzVyPUCuNVY1PDdizszN NFH0T|JUSU6JRWK=
Ramos-2G6-4C10 MoDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mor5TWM2OD1yLkG2PVg{KM7:TR?= M3\Y[nNCVkeHUh?=
NBsusSR MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\WcotLUUN3ME2wMlE4ODF7IN88US=> M3W0[HNCVkeHUh?=
LAMA-84 NETlNWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1vJTWlEPTB;MD6xPFA{OiEQvF2= NHPmTZdUSU6JRWK=
NCI-H1581 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPQTWlLUUN3ME2wMlE5QTZ4IN88US=> Mmr6V2FPT0WU
A204 MlrvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmT2TWM2OD1yLkG4PVch|ryP MoewV2FPT0WU
A549 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTBwMUm1OUDPxE1? NUXucFk4W0GQR1XS
CAL-51 M3f3WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7VTWM2OD1yLkG5OlUyKM7:TR?= MlPNV2FPT0WU
NKM-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXaTnNKSzVyPUCuNlAxOjlizszN NWqxOYFTW0GQR1XS
LoVo NITHeVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfOTWM2OD1yLkKwNlAzKM7:TR?= NF3EWHNUSU6JRWK=
HAL-01 NIDUcYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHPOYFKSzVyPUCuNlAzQTdizszN M2T6cHNCVkeHUh?=
HCC2218 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHniTZpKSzVyPUCuNlA3QDhizszN NX\ZRYNWW0GQR1XS
BEN MkfSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTBwMkGyPVMh|ryP MlHTV2FPT0WU
LC-2-ad MknIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPnTWM2OD1yLkKyNFg1KM7:TR?= NWPP[WxTW0GQR1XS
LXF-289 NFP2coNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVX2RYxZUUN3ME2wMlI2ODRzIN88US=> NIDHPGJUSU6JRWK=
HGC-27 NYXaN2I6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1n3U2lEPTB;MD6yOlQyPCEQvF2= MX\TRW5ITVJ?
LK-2 Mlj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTBwMk[4NlQh|ryP NIX6ZYVUSU6JRWK=
CTB-1 MlTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDLeWRwUUN3ME2wMlI4OjF3IN88US=> M3G1fHNCVkeHUh?=
697 NVfKR2pIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTBwMkezOlch|ryP MYPTRW5ITVJ?
ES7 NF;GUZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTBwMkezPFMh|ryP MXzTRW5ITVJ?
MG-63 MlXOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTBwMke0NFch|ryP MVrTRW5ITVJ?
T47D M2DWVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIqxUlRKSzVyPUCuNlc1PzVizszN MVzTRW5ITVJ?
RPMI-8226 NFTkRZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTBwMki1PFkh|ryP M33iO3NCVkeHUh?=
NCI-H292 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zkSGlEPTB;MD6yPVE3PCEQvF2= M4n5UnNCVkeHUh?=
HT MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPY[ndDUUN3ME2wMlI6OzhizszN Mn:xV2FPT0WU
A3-KAW MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jLO2lEPTB;MD6yPVc1KM7:TR?= MXTTRW5ITVJ?
LB2241-RCC MnrvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rFNGlEPTB;MD6zNFE6QCEQvF2= NEjEXXZUSU6JRWK=
BB65-RCC NHSxeJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWX0e4dZUUN3ME2wMlMxQDB3IN88US=> Mn76V2FPT0WU
G-401 M3zRc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoW2TWM2OD1yLkOwPVg3KM7:TR?= NYX0bmFRW0GQR1XS
TYK-nu MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYP0fpJwUUN3ME2wMlMyQDZ2IN88US=> NWDtSHo3W0GQR1XS
NCI-SNU-1 NFHnboVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTBwM{KzN|Eh|ryP NGf6N4tUSU6JRWK=
CHL-1 M1\qdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3O2bmlEPTB;MD6zNlcxOyEQvF2= M333[HNCVkeHUh?=
P30-OHK MnKwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1vtU2lEPTB;MD6zNlcxPSEQvF2= NYnwXHZrW0GQR1XS
PA-1 M3\VUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTBwM{O2NVkh|ryP M3Tz[nNCVkeHUh?=
LB1047-RCC MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTBwM{SwOFch|ryP NWHScmtKW0GQR1XS
EM-2 MkLPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnuN2gyUUN3ME2wMlM1OTh6IN88US=> MXvTRW5ITVJ?
VA-ES-BJ MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3RR3pKSzVyPUCuN|QzPjhizszN NX3PU402W0GQR1XS
NCI-H520 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWi1XWhIUUN3ME2wMlM1QDN2IN88US=> NVq1VIQzW0GQR1XS
ECC10 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHDT4RjUUN3ME2wMlM2ODN3IN88US=> NVS0OGNDW0GQR1XS
LNCaP-Clone-FGC MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7rTWM2OD1yLkO1N|E1KM7:TR?= M1vwdHNCVkeHUh?=
ES8 NUnGbmp4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;MTWM2OD1yLkO2OVU{KM7:TR?= NVXj[oloW0GQR1XS
HuO-3N1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlSzTWM2OD1yLkO4N|c6KM7:TR?= M{HXbHNCVkeHUh?=
L-363 NInn[pBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTBwM{mwNlYh|ryP M2qyUnNCVkeHUh?=
SK-LU-1 MontS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFT5bpFKSzVyPUCuN|kzODhizszN M1S3NXNCVkeHUh?=
MCF7 NUnqSHo4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3lVnJ6UUN3ME2wMlQxPTNizszN MmDYV2FPT0WU
HuO9 MleyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWP1dpRDUUN3ME2wMlQxPjZ5IN88US=> MVLTRW5ITVJ?
BHT-101 NU\2Z4xGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLYTWM2OD1yLkSwO|g4KM7:TR?= NHW3c4VUSU6JRWK=
MZ2-MEL Mn;hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nTOWlEPTB;MD60NFg5OiEQvF2= Mk\ZV2FPT0WU
NOS-1 NHHtN5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX[4b2JPUUN3ME2wMlQyODd2IN88US=> M{TrS3NCVkeHUh?=
NEC8 MoLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{j0cWlEPTB;MD60NVY6PSEQvF2= MVfTRW5ITVJ?
COR-L88 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTBwNEKyOlch|ryP M4fs[nNCVkeHUh?=
MFM-223 M1nnd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{DM[2lEPTB;MD60NlYyPyEQvF2= M{[wT3NCVkeHUh?=
HSC-3 NX7Ob21IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;MU2lEPTB;MD60NlkxQCEQvF2= NIfWeVBUSU6JRWK=
OVCAR-5 NGnIcnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXyTWM2OD1yLkSyPVM{KM7:TR?= M3ns[3NCVkeHUh?=
MFE-280 MlzJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXH3bGNqUUN3ME2wMlQ{ODN{IN88US=> MVzTRW5ITVJ?
KP-N-YS NEiyO|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnX[XhuUUN3ME2wMlQ{ODdizszN NGPqcopUSU6JRWK=
EPLC-272H MmXpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfpWXZ1UUN3ME2wMlQ{ODd5IN88US=> M2T0VHNCVkeHUh?=
KE-37 NGjVPIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3mxR2lEPTB;MD60N|I1PyEQvF2= NWm5Tm5WW0GQR1XS
HuH-7 Mn76S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7xTWM2OD1yLkSzN|czKM7:TR?= NFLjcYNUSU6JRWK=
CHP-212 NFnzeYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLjb41lUUN3ME2wMlQ{Pjl5IN88US=> NVLuN5BUW0GQR1XS
SK-MES-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmP1TWM2OD1yLkSzPFQ5KM7:TR?= NV2zPIJlW0GQR1XS
SJSA-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{H4W2lEPTB;MD60OFU2PCEQvF2= NYnrNZBUW0GQR1XS
QIMR-WIL MlvqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHe4PWdKSzVyPUCuOFUyPSEQvF2= M1raS3NCVkeHUh?=
ES4 M1qwZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTBwNEWzN|Yh|ryP MojVV2FPT0WU
TI-73 MoLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVm3OVlIUUN3ME2wMlQ2Pjh4IN88US=> MXjTRW5ITVJ?
COLO-684 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvsbWhKSzVyPUCuOFU4OjZizszN MYPTRW5ITVJ?
GCIY MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTBwNEW3OVQh|ryP M2DCS3NCVkeHUh?=
JVM-3 MkD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELQS|hKSzVyPUCuOFYzQTJizszN MXnTRW5ITVJ?
MPP-89 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;tTWM2OD1yLkS2PVQ4KM7:TR?= MmnaV2FPT0WU
M14 MoLQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYCzWFk3UUN3ME2wMlQ4OTdizszN M3THUnNCVkeHUh?=
SW962 NUH0VoptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnabZRKSzVyPUCuOFcyQSEQvF2= MlnTV2FPT0WU
VM-CUB-1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33OO2lEPTB;MD60O|I5PiEQvF2= M4ezNnNCVkeHUh?=
A101D NFrET5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjUOFZKSzVyPUCuOFg6PjhizszN NI\ac3hUSU6JRWK=
DB NVTCTYFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFf3V3ZKSzVyPUCuOVAxOjFizszN NYm1VpE4W0GQR1XS
KNS-62 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDjTWM2OD1yLkWwOVY5KM7:TR?= NVPqSpdDW0GQR1XS
NCI-H460 NWqyNpZIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mly4TWM2OD1yLkWwOVk4KM7:TR?= NYXqNmNJW0GQR1XS
MDA-MB-361 Mk\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTBwNUC4O{DPxE1? NX2xR2NZW0GQR1XS
DOK M{SxSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzl[HpMUUN3ME2wMlUyOzN|IN88US=> MX\TRW5ITVJ?
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BT-474 M4SwN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTFwN{ixO|Eh|ryP NVTrVnpHW0GQR1XS
KM12 NYP1NIJ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\wVYZLUUN3ME2xMlc6PzRzIN88US=> MkTKV2FPT0WU
COLO-680N M3u3fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3jFUGlEPTB;MT64NlM5OSEQvF2= M3H0d3NCVkeHUh?=
Saos-2 M1:2d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjXT29KSzVyPUGuPFI1PDVizszN NHjod2VUSU6JRWK=
CAL-54 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojjTWM2OD1zLkizN|E{KM7:TR?= MYLTRW5ITVJ?
U-118-MG NF\KeYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF3VWVhKSzVyPUGuPFQ3OjJizszN Ml7YV2FPT0WU
TGBC24TKB NVnPRoRkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnTS3ZKSzVyPUGuPFU2ODFizszN NVXTUo1vW0GQR1XS
NCI-H2052 NEf6XXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7VTWM2OD1zLki2OFEh|ryP MmjCV2FPT0WU
BB30-HNC MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTFwOE[0OlIh|ryP NEnadpVUSU6JRWK=
KNS-81-FD NE\VN3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTFwOEmxOlUh|ryP M{nCeXNCVkeHUh?=
HN MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jpZ2lEPTB;MT64PVU1PSEQvF2= MV;TRW5ITVJ?
ACHN M1\WVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTFwOUC3PFYh|ryP MWXTRW5ITVJ?
HSC-2 Ml;3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTFwOUGwPVYh|ryP M3HDeXNCVkeHUh?=
HCC2998 NIroVWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGG5cXRKSzVyPUGuPVM{OjVizszN MWPTRW5ITVJ?
HEL M3zVR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPkRoNKSzVyPUGuPVQyQDRizszN M4T1VHNCVkeHUh?=
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8-MG-BA MknTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVuxblZ5UUN3ME2xMlk5OjFizszN NUm3O5VMW0GQR1XS
OVCAR-8 NIHJT|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3SzOmlEPTB;Mj6wNFI6PSEQvF2= NWjY[GJbW0GQR1XS
DSH1 NESwfopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDoT5pKSzVyPUKuNFE4PzRizszN NX3hN2V3W0GQR1XS
KYSE-520 NH3LVppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fke2lEPTB;Mj6wNlA1QSEQvF2= MYTTRW5ITVJ?
GB-1 NH7jVHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\z[IRKSzVyPUKuNFI2PzdizszN MWXTRW5ITVJ?
RCC10RGB MkjYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfLWYNKSzVyPUKuNFQ5PzFizszN MXnTRW5ITVJ?
SW954 NWnpRYFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2ftN2lEPTB;Mj6wOVA{PiEQvF2= NFzjWFZUSU6JRWK=
IST-MES1 Mn76S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33lemlEPTB;Mj6wOVU3QCEQvF2= M{D5cHNCVkeHUh?=
AU565 MkDyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3JVllKSzVyPUKuNFU6ODVizszN NEnWb|hUSU6JRWK=
CW-2 Ml\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\KNGlEPTB;Mj6wOlg4PSEQvF2= MWfTRW5ITVJ?
TE-1 Ml\aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTJwMEmzO|ch|ryP M4P5SHNCVkeHUh?=
KP-4 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFrlPVVKSzVyPUKuNVA{OTJizszN NG\JXnBUSU6JRWK=
SNU-C2B NFzwR29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX3XblZ1UUN3ME2yMlExQDJ|IN88US=> MVXTRW5ITVJ?
NCI-H1395 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTJwMUC5PFYh|ryP NF\PW|VUSU6JRWK=
CAS-1 NVXFdFhwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTJwMUG1OFYh|ryP M3HI[nNCVkeHUh?=
NCI-H1155 NV\3PVZ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYL3W|d1UUN3ME2yMlIzPjlizszN MXXTRW5ITVJ?
SK-MEL-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3fUTWlEPTB;Mj6yPFE5OSEQvF2= NHPnSmtUSU6JRWK=
KINGS-1 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVz4[o1NUUN3ME2yMlMxODh6IN88US=> NXz1cnpZW0GQR1XS
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PANC-10-05 MmDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjHTWM2OD1{LkO3NFI1KM7:TR?= Mlu4V2FPT0WU
HT-1376 MofXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWW3UmlWUUN3ME2yMlM5PzB6IN88US=> MVjTRW5ITVJ?
LS-513 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrmepVKSzVyPUKuN|g5PzRizszN NEfTe21USU6JRWK=
KNS-42 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTJwM{mxNFUh|ryP NYnZO2V{W0GQR1XS
639-V MmjvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDyTWM2OD1{LkSyO|U5KM7:TR?= MX3TRW5ITVJ?
SJRH30 M4fZW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTJwNEWxOlEh|ryP NF:5eHFUSU6JRWK=
SW1783 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXizRWF7UUN3ME2yMlQ4ODd6IN88US=> M17tfnNCVkeHUh?=
NB6 MlnTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXtTWM2OD1{LkS4Nlgh|ryP MWLTRW5ITVJ?
SK-MEL-30 NYHMclRXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUTJR|UxRTJwNEi4N{DPxE1? NXvoT|lVW0GQR1XS
HCE-4 NITFXmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mln5TWM2OD1{LkS5OFAzKM7:TR?= M1:0bXNCVkeHUh?=
NB10 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjkdJBMUUN3ME2yMlQ6PDJ7IN88US=> M3TNR3NCVkeHUh?=
H-EMC-SS Mo\hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTJwNUS2OUDPxE1? M{\4OnNCVkeHUh?=
SK-HEP-1 M4ewXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTJwNUWyN|Yh|ryP Ml;BV2FPT0WU
HCC1395 MnnkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXrTWM2OD1{LkW1OlU2KM7:TR?= NF;o[m9USU6JRWK=
HuCCT1 M3LGdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTJwNU[zPUDPxE1? NIX5VpRUSU6JRWK=
SiHa NEDvdYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTJwNUi3NVkh|ryP MWTTRW5ITVJ?
EFO-21 Mn7LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnVNpRKSzVyPUKuOVg4PjRizszN M4HsUHNCVkeHUh?=
NCI-H1666 NG\vc4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTJwNUmwO|Yh|ryP NVnzcFhtW0GQR1XS
UMC-11 NUfhVG9wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjaZW9lUUN3ME2yMlU6QDZ6IN88US=> NHq3dVJUSU6JRWK=
COLO-679 NHLGNpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFq5do5KSzVyPUKuOlEzOzNizszN NFPFOoZUSU6JRWK=
A388 M3vDN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTJwNkWyOVMh|ryP M33FNXNCVkeHUh?=
LB2518-MEL NEfa[o1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDRVVJZUUN3ME2yMlY2PDB6IN88US=> Mn\1V2FPT0WU
EW-13 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFTROFdKSzVyPUKuOlg4PjhizszN MWrTRW5ITVJ?
MLMA MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnlfI5KSzVyPUKuOlg5PTZizszN NXvacZo1W0GQR1XS
HOP-92 NYGzbIpZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTJwN{CxNUDPxE1? NXn4fFhNW0GQR1XS
NOMO-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{XpfWlEPTB;Mj63NVQ1OiEQvF2= NFzZS3pUSU6JRWK=
NCI-H810 NYK4PIRjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLV[oUzUUN3ME2yMlczOTl6IN88US=> MV3TRW5ITVJ?
769-P NHXnfmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;QTWM2OD1{LkeyNlgh|ryP NEHVSJVUSU6JRWK=
SAS Mn\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXBT2lnUUN3ME2yMlc3PSEQvF2= MlzXV2FPT0WU
M059J MmHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHnbWZSUUN3ME2yMlc5OzF6IN88US=> MV7TRW5ITVJ?
RD M{\pVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jt[mlEPTB;Mj63PVQ4OSEQvF2= NF3qb|RUSU6JRWK=
GT3TKB M{CzXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUn1OIdZUUN3ME2yMlg{PDN|IN88US=> MoSxV2FPT0WU
MZ7-mel MnmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXCRVZKSzVyPUKuPFY2ODJizszN NVThZ5NEW0GQR1XS
KYSE-510 NV7oS3ZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPrTWM2OD1{Lki5OVA1KM7:TR?= NH7tRopUSU6JRWK=
NCI-N87 MonpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTJwOUG4NFch|ryP MlvTV2FPT0WU
MKN1 NFvheZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fx[GlEPTB;Mz6wNFE3OyEQvF2= NHHjNJlUSU6JRWK=
NCI-H2170 M17Dc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LwZmlEPTB;Mz6wNFQ2OSEQvF2= MVPTRW5ITVJ?
OVCAR-3 NE\4eYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M134e2lEPTB;Mz6wNlEyPSEQvF2= NXHFeohxW0GQR1XS
KLE MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHxTWM2OD1|LkC2PFEzKM7:TR?= MX3TRW5ITVJ?
LB771-HNC NYHIe5U3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\XfmlEPTB;Mz6wPVM1OyEQvF2= MWDTRW5ITVJ?
MC116 MkLES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXydplKSzVyPUOuNVEyOTJizszN NHPmWHpUSU6JRWK=
SCC-9 Mn;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;Pc3lKSzVyPUOuNVE{PjZizszN MX;TRW5ITVJ?
U031 M4[wWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTNwMUK0NlMh|ryP NXqz[mdkW0GQR1XS
LCLC-103H M3r1Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHv[lJKSzVyPUOuNlM5QTRizszN MYDTRW5ITVJ?
U-266 NYj6R|dwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUK0VZBCUUN3ME2zMlI4PjN|IN88US=> NVTaVXlyW0GQR1XS
JVM-2 M3PoXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITBPGFKSzVyPUOuN|IyPjRizszN NEj3N|ZUSU6JRWK=
UACC-257 M{PoW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nYfWlEPTB;Mz60OlQ{OSEQvF2= NFPq[GVUSU6JRWK=
LB996-RCC M3mzS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrFTWM2OD1|LkS2OVAzKM7:TR?= MWfTRW5ITVJ?
BT-549 NV;hPVZET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHqxbFJKSzVyPUOuOVAzODlizszN NVjVSIhyW0GQR1XS
NCI-H526 MliwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLITWM2OD1|LkW0NVU5KM7:TR?= MWHTRW5ITVJ?
RH-18 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTNwNkG3PVgh|ryP M{iwSHNCVkeHUh?=
SW626 M2PNXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEPibGJKSzVyPUOuO|EyOjhizszN Ml;FV2FPT0WU
SK-N-AS NF3VNXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvFVpBKSzVyPUOuO|MzPDdizszN M3PaUHNCVkeHUh?=
IGR-1 NHHwcYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknYTWM2OD1|Lke0NlQ5KM7:TR?= NFjFTIZUSU6JRWK=
NCI-H2291 M3TXdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTNwN{WzN|Yh|ryP M4PuNnNCVkeHUh?=
NCI-H441 Ml3lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTNwOEK0N|Mh|ryP NXXmUpdYW0GQR1XS
D-247MG M33U[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlG4TWM2OD1|Lki2NFMyKM7:TR?= NXTXWId4W0GQR1XS
COLO-320-HSR NYDCOI5NT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLtTWM2OD1|LkmxNlcyKM7:TR?= NW\vNWs5W0GQR1XS
A253 NIqzR5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1W2TmlEPTB;Mz65NVQ{PiEQvF2= MVLTRW5ITVJ?
HT55 NVrsXmFCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7YbGczUUN3ME2zMlkyPzd|IN88US=> NHX1XIFUSU6JRWK=
MZ1-PC MmnRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTNwOUW4OlQh|ryP NV7sZ2szW0GQR1XS
RERF-LC-MS NUnPN|duT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\tVmlEPTB;Mz65OlUzPSEQvF2= NFXBfnBUSU6JRWK=
SF268 M4PJN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjCfWlNUUN3ME20MlEzQDhzIN88US=> M2nOZXNCVkeHUh?=
RMG-I NFvHS5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnJPVR[UUN3ME20MlIyPDV2IN88US=> MlPFV2FPT0WU
CFPAC-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDrTWM2OD12LkKxOlg2KM7:TR?= M3eyT3NCVkeHUh?=
S-117 M2rmd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrxXVFKSzVyPUSuNlIxOjRizszN NV7HOHQzW0GQR1XS
NCI-H2122 NHTZ[lZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NES0UWJKSzVyPUSuN|A6OzFizszN NV7oOHdwW0GQR1XS
J82 M1;HNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\0TWM2OD12LkOzOFQyKM7:TR?= MkXBV2FPT0WU
CAL-72 NX;0[YtGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjGN|RkUUN3ME20MlM2PDd5IN88US=> MlrFV2FPT0WU
GP5d MnLES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILUdmxKSzVyPUSuOFEzQThizszN Mmi1V2FPT0WU
UACC-62 Mn7lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn33TWM2OD12LkS2OVQ2KM7:TR?= M4\HeHNCVkeHUh?=
MS-1 M3nidmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTRwNEmxNVUh|ryP NIT5PVNUSU6JRWK=
RCM-1 NGTyVVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;rXZdKSzVyPUSuOVI6OiEQvF2= NYfMUJFpW0GQR1XS
IST-SL1 NV;5OWhxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHv5TppKSzVyPUSuOlU{PTlizszN NX;GcG1bW0GQR1XS
SW756 M3PyW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\UTWM2OD12Lk[5NVM2KM7:TR?= NX72Om02W0GQR1XS
HCC38 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfaXIFKSzVyPUSuO|EyQTdizszN NH\uZWNUSU6JRWK=
YAPC MlPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LS[WlEPTB;ND63NVMyOyEQvF2= MVrTRW5ITVJ?
DK-MG MmDCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH6zUWJKSzVyPUSuO|gyOTJizszN MlPUV2FPT0WU
HCC1937 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\BTWM2OD12Lki1NVI2KM7:TR?= M33qV3NCVkeHUh?=
OAW-28 NFjUN2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnwN2h7UUN3ME20MlkzPDN3IN88US=> MVvTRW5ITVJ?
MEG-01 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHH4TWtKSzVyPUWuNFg{OjhizszN NUTuR2M6W0GQR1XS
GI-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXjTWM2OD13LkKzOFg3KM7:TR?= MVPTRW5ITVJ?
FADU MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELJd4ZKSzVyPUWuNlc5OzdizszN M{TkNXNCVkeHUh?=
CAPAN-1 NVHTc297T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTVwM{m0N|Ih|ryP MlfSV2FPT0WU
TGBC11TKB MoT4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTVwNEW3PFYh|ryP MYHTRW5ITVJ?
DBTRG-05MG MlHkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVz5V2tqUUN3ME21MlUxPTJizszN NVr5c|BbW0GQR1XS
ONS-76 NFz0VYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPIcHhKSzVyPUWuOVU5ODFizszN Mn3LV2FPT0WU
Ca-Ski NHXoWHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTVwNkK1OlYh|ryP NFvicHpUSU6JRWK=
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D-392MG MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\QdWlxUUN3ME22MlM{Ozl7IN88US=> NXLFdFhlW0GQR1XS
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HCC2157 NIrpVHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnr2TWM2OD14LkS1OlE3KM7:TR?= NFj4THNUSU6JRWK=
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T47D-tamR  NVjDdJFTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTF7IH7N MlLxNlQ1PTdyNkm=
293T  MnjySpVv[3Srb36gRZN{[Xl? MV:yNEBvVQ>? M1HuWFMhcA>? NV7rXnZy\Gm|coXweJMhSWu2LX3l[IlifGWmIGTJSk1KSSC|dHHibYxqfHluIITyZY5{dG:lYYTpc44tKGGwZDDhZ5Rqfmm2eR?= NEO1fo8zPDN4M{S0PS=>
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Huh7  NFjlTlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVu1MVExOCCwTR?= MWCwMVI1KGh? M2Pse2ROW09? NHLNV4JqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NYTvO4hkOjR{OUezNFA>
Hep3B  M4G0d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3:5ZlUuOTByIH7N M{LZflAuOjRiaB?= MWDEUXNQ NUHFPIFocW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ MVmyOFI6PzNyMB?=
Huh7  MkjMSpVv[3Srb36gRZN{[Xl? MYmxNFAhdk1? M1i4WVEzKGh? MoLsSG1UVw>? NGm2OYNqdmS3Y3XzJIF2fG:yaHHnfeKh MnqwNlQzQTd|MEC=
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RMS13 MYnBdI9xfG:|aYOgRZN{[Xl? MnvDNUDPxE1? NV;Gd41CPDhiaB?= M3znZolv[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JGFDXC15M{e= NYXySlJFOjRzM{OyNVg>
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Rh41 M2XVdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTNyLkegcm0> NXKzc3k{OjF|M{e2O|k>
Rh18 M1nMOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTRzLkSgcm0> NGS1NWkzOTN|N{[3PS=>
Rh30 NIHYe3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfs[W13UUN3ME2xOEBvVQ>? NITsUVgzOTN|N{[3PS=>
BT-12 NGnDVZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHy1NGNKSzVyPUK0Mlchdk1? Mn;yNlE{Ozd4N{m=
CHLA-266 NUn2ZW9JT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTFwMTDuUS=> MmrkNlE{Ozd4N{m=
TC-71 M4nRVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LJbWlEPTB;NE[uOUBvVQ>? NVvCc5dGOjF|M{e2O|k>
CHLA-9 NVvSTYRsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTF3LkOgcm0> NFfFUYkzOTN|N{[3PS=>
CHLA-10 MlnuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWO3NXQ2UUN3ME2zMlkhdk1? MXeyNVM{PzZ5OR?=
CHLA-258 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGD1O2RKSzVyPUGwMlQhdk1? MoLTNlE{Ozd4N{m=
GBM2 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrCU|J4UUN3ME20NE44KG6P MYCyNVM{PzZ5OR?=
NB-1643 NUDQUYszT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV2zZWNmUUN3ME2zN{45KG6P NHTabngzOTN|N{[3PS=>
NB-EBc1 M2D0dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorOTWM2OD1{Nz6xJI5O MmXFNlE{Ozd4N{m=
CHLA-90 NH;uRWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TsRWlEPTB;M{SuOUBvVQ>? NELU[4czOTN|N{[3PS=>
CHLA-136 MkC4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HSOmlEPTB;MU[yMlYhdk1? Mm\PNlE{Ozd4N{m=
NALM-6 MkLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk[wTWM2OD1|Mj65JI5O NGfzfWQzOTN|N{[3PS=>
COG-LL-317 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLGTWM2OD1zLkOgcm0> MYKyNVM{PzZ5OR?=
RS4;11 NXK2Rm1ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTN3IH7N MmjzNlE{Ozd4N{m=
MOLT-4 M2nV[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1rFWmlEPTB;OD62JI5O M2LGNVIyOzN5Nke5
CCRF-CEM M3q5dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XSW2lEPTB;ND6xJI5O MlP6NlE{Ozd4N{m=
Kasumi-1 NEfsNWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTFwNDDuUS=> NX\YS4hEOjF|M{e2O|k>
Karpas-299 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnsTWM2OD1{MT63JI5O NGHtXmozOTN|N{[3PS=>
Ramos-RA1 NGPGdY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWGyZolpUUN3ME24MlIhdk1? M4\DOVIyOzN5Nke5
H838 MXTGeY5kfGmxbjDBd5NigQ>? NEGyPIcyKM7:TR?= MlLYOFghcA>? Ml63bY5lfWOnczDj[YxtKGG3dH;wbIFogQ>? NVjqUJZbOjB|NkSxNVM>
H838 NHHnN|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXewMVMh|ryP Moe2O|IhcA>? NWj3dpA2cW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 MYSyNFAzQDh3NB?=
A549  Mlz1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mkj5NE0{KM7:TR?= MUm3NkBp MnzQbY5lfWOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh[XW2b4DoZYd6 M4C1ZlIxODJ6OEW0

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca null Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID