AZD8055

Catalog No.S1555

For research use only.

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.

AZD8055 Chemical Structure

CAS No. 1009298-09-2

Selleck's AZD8055 has been cited by 216 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Other mTOR Products

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTBwMEKzN|Eh|ryP NX3PV3lQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
NCI-H720 NWjXO3BOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYGzeXNjUUN3ME2wMlAzQTN6IN88US=> NX;3Wlk1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
KARPAS-45 Mk\NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;qVHNvUUN3ME2wMlA{OzdzIN88US=> M4\S[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
EW-3 M3TZdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETSTnRKSzVyPUCuNFQxPTZizszN NGXH[Wg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
SBC-1 Mn;SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPZTWM2OD1yLkC0OVgyKM7:TR?= MoDHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
NCI-H209 NX;tfmRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjXTGRYUUN3ME2wMlA2PDV|IN88US=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
EoL-1-cell MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\wWWlEPTB;MD6wOlU3QSEQvF2= Mm\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
H9 NXi2fFFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHsPZNNUUN3ME2wMlA3Pjl{IN88US=> M1jNbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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MV-4-11 MnG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33OdWlEPTB;MD6xNFM1PyEQvF2= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
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A3-KAW NXPEUHp5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIXvbpZKSzVyPUCuNlk4PCEQvF2= NF\ndoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
LB2241-RCC NUnaemdiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrwToRnUUN3ME2wMlMxOTl6IN88US=> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
BB65-RCC MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoiwTWM2OD1yLkOwPFA2KM7:TR?= NGXoPFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
G-401 M37TfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrZfm0{UUN3ME2wMlMxQTh4IN88US=> M3\ET|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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NCI-SNU-1 NXrOepBVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{Hwc2lEPTB;MD6zNlM{OSEQvF2= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
CHL-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWGzNms2UUN3ME2wMlMzPzB|IN88US=> NEW1epE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
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LB1047-RCC NVzUOVRZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\6ZXN2UUN3ME2wMlM1ODR5IN88US=> MkHXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
EM-2 M{Dsd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M12xOGlEPTB;MD6zOFE5QCEQvF2= M4KzZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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NCI-H520 NXXxWJBIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\URYc{UUN3ME2wMlM1QDN2IN88US=> NGn4VIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
ECC10 MnXYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn:1TWM2OD1yLkO1NFM2KM7:TR?= NG[wVJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
LNCaP-Clone-FGC MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLlTWM2OD1yLkO1N|E1KM7:TR?= MkS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
ES8 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3oTWM2OD1yLkO2OVU{KM7:TR?= NHn4cmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
HuO-3N1 NI\QcW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY\BT5p5UUN3ME2wMlM5Ozd7IN88US=> MmX0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
L-363 NEfVfolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTBwM{mwNlYh|ryP NXrnZoxzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
SK-LU-1 NYW3fG5DT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDSTWM2OD1yLkO5NlA5KM7:TR?= MmfrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
MCF7 M3SzNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\5Rm5KSzVyPUCuOFA2OyEQvF2= MnPaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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BHT-101 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTBwNEC3PFch|ryP NFTaOXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
MZ2-MEL NW\2O4ZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zQXmlEPTB;MD60NFg5OiEQvF2= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NOS-1 NUXzb5F7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVTnZoQ3UUN3ME2wMlQyODd2IN88US=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NEC8 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHScYk5UUN3ME2wMlQyPjl3IN88US=> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
COR-L88 NGW1NlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XWSWlEPTB;MD60NlI3PyEQvF2= NULmbFJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
MFM-223 MmHvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlziTWM2OD1yLkSyOlE4KM7:TR?= M2HDW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
HSC-3 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHW1W3RKSzVyPUCuOFI6ODhizszN M2[wSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
OVCAR-5 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlj4TWM2OD1yLkSyPVM{KM7:TR?= NFHMWow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
MFE-280 NYDHSo83T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrXVFF6UUN3ME2wMlQ{ODN{IN88US=> MmTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
KP-N-YS NIf5U45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX\4[VlqUUN3ME2wMlQ{ODdizszN NVzNdI9HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
EPLC-272H M2O2S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XsTGlEPTB;MD60N|A4PyEQvF2= MnvIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
KE-37 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTBwNEOyOFch|ryP NYrlZXVwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
HuH-7 NGDacm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTBwNEOzO|Ih|ryP MkLrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
CHP-212 M3Wwcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXjTWM2OD1yLkSzOlk4KM7:TR?= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
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SJSA-1 MlnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;TTWM2OD1yLkS0OVU1KM7:TR?= MkjuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
QIMR-WIL NHG2[VdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TvemlEPTB;MD60OVE2KM7:TR?= NWP0O4ZlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
ES4 M1u5VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrQem1ZUUN3ME2wMlQ2OzN4IN88US=> NXvMUphVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
TI-73 NHjxNYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzkTmg{UUN3ME2wMlQ2Pjh4IN88US=> Mmr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
COLO-684 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4ixdWlEPTB;MD60OVczPiEQvF2= NYDD[pZIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
GCIY NFe0RXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;0SldKSzVyPUCuOFU4PTRizszN NHHwUIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
JVM-3 Mn\6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoCxTWM2OD1yLkS2NlkzKM7:TR?= NVvLcYVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
MPP-89 NEWxUI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTBwNE[5OFch|ryP NWXKcHdYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
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TE-9 M3vMUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3\iV2lEPTB;MUeuOVE3PiEQvF2= NX3UTplGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
TE-10 NXn6XHh2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX[wWG1VUUN3ME2xPU4xPjB5IN88US=> NXziOo0yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
COLO-824 NHfxeGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTJyLkmyO|Yh|ryP NX7FVXNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
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KALS-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;LPWlEPTB;MkGuNVc2KM7:TR?= MnTpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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DoTc2-4510 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PSeGlEPTB;MkOuPFQ4PSEQvF2= NWqySpdiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
ABC-1 MoSwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfrWWxKSzVyPUK2Mlc6PTdizszN MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
MHH-NB-11 NYH3WVBQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVm1RmpFUUN3ME2zN{4{PDZ2IN88US=> M{Lt[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
SW1463 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3Tne2lEPTB;M{OuPVg{KM7:TR?= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
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MCF7-X  Mln0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vwe2lEPTB;MkSgcm0> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3N{C2PUc,OjR2NUewOlk9N2F-
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293T  NXy2OZdjTnWwY4Tpc44hSXO|YYm= M{HubFIxKG6P NFq5bmk{KGh? M3LoRYRqe3K3cITzJGFsfC2vZXTpZZRm\CCWSV[tTWEhe3SjYnnsbZR6NCC2cnHud4xw[2G2aX;uMEBidmRiYXP0bZZqfHl? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4M{S0PUc,OjR|NkO0OFk9N2F-
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Huh7  M4DJN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlTNOU0yODBibl2= M3LNeVAuOjRiaB?= NH3TUZlFVVOR NEXobIhqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NXO3WWQzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVc{ODBpPkK0Nlk4OzByPD;hQi=>
Hep3B  MlHBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTuT5c2PS1zMECgcm0> MYWwMVI1KGh? NVX0elZGTE2VTx?= MX;pcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> Mn\IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OUezNFAoRjJ2Mkm3N|AxRC:jPh?=
Huh7  NHPoOFBHfW6ldHnvckBCe3OjeR?= MoLFNVAxKG6P M{DrbFEzKGh? NYPXN|JnTE2VTx?= NULafWh1cW6mdXPld{BifXSxcHjh[5nDqA>? MlrIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OUezNFAoRjJ2Mkm3N|AxRC:jPh?=
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Hep-2 NGnSZY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWOwMlAyNTFizszN NIqwdXIyOi17NjDo MXrpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyM{e0PUc,OjRzMEO3OFk9N2F-
SCC-9 MknIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFz0dlcxNjBzLUGg{txO NF3GOGQyOi17NjDo Ml:0bY5kemWjc3XzJINmdGxiZHXheIghcW5iYTDjc45k\W62cnH0bY9vNSCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVz NFy0SZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGwN|c1QSd-MkSxNFM4PDl:L3G+
Hep-2 NX;ZTmYyTnWwY4Tpc44hSXO|YYm= MljnNE4yNzBwNTFOwG0> M1PVdlI1KGh? NGj3OHNjdG:la4OgcXRQWkNzIHHu[EBuXE:UQ{KgZYN1cX[jdHnvci=> MofqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMEO3OFkoRjJ2MUCzO|Q6RC:jPh?=
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MOLT-4 MkHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRThwNjDuUS=> NHr0bII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
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Ramos-RA1 NVn0[Wl[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3H0[2lEPTB;OD6yJI5O MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
H838 M{nCUWZ2dmO2aX;uJGF{e2G7 NH3NeWgyKM7:TR?= NYTzTYVRPDhiaB?= M4LCV4lv\HWlZYOgZ4VtdCCjdYTvdIhi\3l? MmLYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|NkSxNVMoRjJyM{[0NVE{RC:jPh?=
H838 NGLCR4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzENE0{KM7:TR?= Mkm0O|IhcA>? NXPLdFZ4cW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 M4i1ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEK4PFU1Lz5{MECyPFg2PDxxYU6=
A549  M4nsSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NESxXHAxNTNizszN MkDGO|IhcA>? NIXZSVdqdmS3Y3XzJIdzd3e2aDDpcohq[mm2aX;uJIFv\CCjdYTvdIhi\3l? NUCzTnBsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwNlg5PTRpPkKwNFI5QDV2PD;hQi=>
Assay
Methods Test Index PMID
Western blot p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40 ; p-EGFR / EGFR ; p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a 24457069 25654224
Immunofluorescence p-4EBP1 / EIF4E / mTOR ; LC3B / p62 24600487 30337536
Growth inhibition assay Cell viability 29263324
In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol (from reference)

Kinase Assay:[1]
  • Cell-based determinations of mTOR inhibition:

    A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.

Cell Research:[1]
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 50 mg/mL warmed
(107.4 mM)
Ethanol 3 mg/mL
(6.44 mM)
Water Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1COCCN1C2=NC(=NC3=C2C=CC(=N3)C4=CC(=C(C=C4)OC)CO)N5CCOCC5C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Drug: AZD8055 Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT00999882 Completed Drug: AZD8055 Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Drug: AZD8055 Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Drug: AZD8055 Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Drug: AZD8055 Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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