AZD8055

For research use only.

Catalog No.S1555

180 publications

AZD8055 Chemical Structure

CAS No. 1009298-09-2

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.

Size Price Stock Quantity  
10mM (1mL in DMSO) GBP 127 In stock
GBP 71 In stock
GBP 98 In stock
GBP 303 In stock
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Selleck's AZD8055 has been cited by 180 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 NWj4Vo5{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDR[5U3UUN3ME2wMlAzOzNzIN88US=> Mn3sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
NCI-H720 NIHwZ5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHyV4tLUUN3ME2wMlAzQTN6IN88US=> NYPsfW5FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
KARPAS-45 M{PRZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkK5TWM2OD1yLkCzN|cyKM7:TR?= NH;kSHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
EW-3 Mn\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHW4fGRKSzVyPUCuNFQxPTZizszN NWj1WIJSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
SBC-1 M1m0XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7NTWM2OD1yLkC0OVgyKM7:TR?= M4fLb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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EoL-1-cell MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjBd3pzUUN3ME2wMlA3PTZ7IN88US=> M3rwU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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AN3-CA Mm[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnOSGlSUUN3ME2wMlA4QTVzIN88US=> MmCxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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BV-173 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DrR2lEPTB;MD6wPVA3OSEQvF2= M1;nTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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G-402 MlLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvDO2ZKSzVyPUCuNFk6QCEQvF2= M4jLRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
MV-4-11 MmrHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTBwMUCzOFch|ryP MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NB69 NIL2NHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGn1eHFKSzVyPUCuNVM2KM7:TR?= M3ywO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
EGI-1 MmL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofRTWM2OD1yLkGzOVMyKM7:TR?= MlzPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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ME-180 NWe0NYc3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPxTWM2OD1yLkG0OlgyKM7:TR?= MkDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
D-283MED MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPSZndKSzVyPUCuNVQ4OjNizszN MnjhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
NB14 M2K2VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3WyZWlEPTB;MD6xOlQ1PyEQvF2= NIjpfpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
Ramos-2G6-4C10 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2faZmlEPTB;MD6xOlk5OyEQvF2= Mn7tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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LAMA-84 M1\jOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jVPWlEPTB;MD6xPFA{OiEQvF2= NFrl[HI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
NCI-H1581 NYO3TpB1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\zTWlWUUN3ME2wMlE5QTZ4IN88US=> NIXCVGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
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A549 M334WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmq0TWM2OD1yLkG5OVUh|ryP MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
CAL-51 NISzN3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfJTWM2OD1yLkG5OlUyKM7:TR?= NYjKe|ZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
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T47D M33VSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnH5TWM2OD1yLkK3OFc2KM7:TR?= M3HycFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
RPMI-8226 NF;HSItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1OwOGlEPTB;MD6yPFU5QSEQvF2= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NCI-H292 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Pm[WlEPTB;MD6yPVE3PCEQvF2= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
HT NUOw[ZlCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;SXXN3UUN3ME2wMlI6OzhizszN NFnlXZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
A3-KAW NFPUSFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;GcGlEPTB;MD6yPVc1KM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
LB2241-RCC MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPTTWM2OD1yLkOwNVk5KM7:TR?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
BB65-RCC NXL1T21mT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLwTWM2OD1yLkOwPFA2KM7:TR?= M3qyS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
G-401 NHrINItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTBwM{C5PFYh|ryP NEDYcIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
TYK-nu M2W4e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIf6Z4VKSzVyPUCuN|E5PjRizszN MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NCI-SNU-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjF[4lKSzVyPUCuN|I{OzFizszN NGPrfo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
CHL-1 Mo\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnJbIxWUUN3ME2wMlMzPzB|IN88US=> M1rpcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
P30-OHK NXzuVm1rT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrKO|dKSzVyPUCuN|I4ODVizszN NHz4b|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
PA-1 MnLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXZdYNKSzVyPUCuN|M3OTlizszN NF3BUVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
LB1047-RCC NV;ZTpA4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTBwM{SwOFch|ryP NXrUO4dwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
EM-2 NVvI[ItPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTBwM{SxPFgh|ryP M17XfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
VA-ES-BJ MoP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHwWmFKSzVyPUCuN|QzPjhizszN NWPtd49PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
NCI-H520 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4Lx[2lEPTB;MD6zOFg{PCEQvF2= NIXUPJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
ECC10 NFTSWo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnxN2JKSzVyPUCuN|UxOzVizszN MnfQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
LNCaP-Clone-FGC M37qWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV[ybm53UUN3ME2wMlM2OzF2IN88US=> MlzxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
ES8 MorqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLXSZpKSzVyPUCuN|Y2PTNizszN M4TqNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
HuO-3N1 NVj1e3JrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTBwM{izO|kh|ryP NV30PZQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
L-363 NWXReFZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTBwM{mwNlYh|ryP M{flUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
SK-LU-1 NYjscmxuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vFSGlEPTB;MD6zPVIxQCEQvF2= NEiwXYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
MCF7 NVrOR49yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTBwNEC1N{DPxE1? NF\MU|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
HuO9 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zWSGlEPTB;MD60NFY3PyEQvF2= M4HDXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
BHT-101 MmXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmq3TWM2OD1yLkSwO|g4KM7:TR?= M2TEfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
MZ2-MEL NUDPOIZIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HBe2lEPTB;MD60NFg5OiEQvF2= NYXZ[W9{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
NOS-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF31d45KSzVyPUCuOFExPzRizszN MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NEC8 MmntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjPTWM2OD1yLkSxOlk2KM7:TR?= M{nTc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
COR-L88 M2KxVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\yW3ZKSzVyPUCuOFIzPjdizszN MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
MFM-223 NH[0TldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTBwNEK2NVch|ryP M{HlfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
HSC-3 NIfaRo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrENVRuUUN3ME2wMlQzQTB6IN88US=> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
OVCAR-5 M2XXc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jiOGlEPTB;MD60Nlk{OyEQvF2= M1;vTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
MFE-280 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3TBUWlEPTB;MD60N|A{OiEQvF2= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
KP-N-YS NXOwbFN[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHn1VIhKSzVyPUCuOFMxPyEQvF2= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
EPLC-272H NVW2WmRmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWH5b49FUUN3ME2wMlQ{ODd5IN88US=> NXXqbm4zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
KE-37 NILkeGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETrcWRKSzVyPUCuOFMzPDdizszN NYWzdHI1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
HuH-7 NIjwVGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4P5SmlEPTB;MD60N|M4OiEQvF2= NWnreY1NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
CHP-212 M3XN[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jJU2lEPTB;MD60N|Y6PyEQvF2= NEfZdGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
SK-MES-1 NFHnfJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37nOWlEPTB;MD60N|g1QCEQvF2= M1rnOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
SJSA-1 MlPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DTRWlEPTB;MD60OFU2PCEQvF2= M1LxSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
QIMR-WIL NVnOPYxbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33M[2lEPTB;MD60OVE2KM7:TR?= MoDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
ES4 M2\Ge2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFq5c5RKSzVyPUCuOFU{OzZizszN MnrwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
TI-73 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTBwNEW2PFYh|ryP M4Hr[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
COLO-684 NEXafHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEL4NFdKSzVyPUCuOFU4OjZizszN NXfQXIVZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
GCIY MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWn6SZZPUUN3ME2wMlQ2PzV2IN88US=> NXLEbVM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
JVM-3 NH\lc3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\oU2lEPTB;MD60OlI6OiEQvF2= M4LScFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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TE-9 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHDTWM2OD1zNz61NVY3KM7:TR?= NETSc5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
TE-10 NFfSeIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWW5XY5NUUN3ME2xPU4xPjB5IN88US=> NEnnUnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
COLO-824 NIPFOlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUixOIg4UUN3ME2yNE46Ojd4IN88US=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
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KALS-1 Mn\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHWVGZKSzVyPUKxMlE4PSEQvF2= NIXUbGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
L-428 NIDjV|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1X6NGlEPTB;MkGuOFAyPiEQvF2= M{TsSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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DoTc2-4510 MlzlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk[1TWM2OD1{Mz64OFc2KM7:TR?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
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MHH-NB-11 NGKwW3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDlTWM2OD1|Mz6zOFY1KM7:TR?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
SW1463 MlLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7zTWM2OD1|Mz65PFMh|ryP NGjDXlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
SW1417 NW\NZ4lYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTN5LkC5OVIh|ryP MnnhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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H1048 NFfmbIlCeG:ydH;zbZMhSXO|YYm= NHPjfHg2ODBibl2= M4TsV|czKGh? NXruRZN3dmijbnPld{BCSlRvMk[z5qCUcW6mdXPl[EBieG:ydH;zbZM> NVziVJVsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3N|c2PDJpPkK1O|M4PTR{PD;hQi=>
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H446 NXvMVotJSXCxcITvd4l{KEG|c3H5 M{DmZlUxOCCwTR?= NIK2[FE4OiCq MVrubIFv[2W|IFHCWE0zPjQkgKPpcoR2[2WmIHHwc5B1d3Orcx?= NVPjT2xCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3N|c2PDJpPkK1O|M4PTR{PD;hQi=>
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MOLM13 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXOwMlIh|ryP NV33[oJYPDhiaB?= MYDlcohidmOnczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGmwZIXj[YQh[nlic3XseY1mfGmwaXK= M{nJXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{O5N|k{Lz5{NEezPVM6OzxxYU6=
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MCF7-X  NIHnSIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIL3PXpKSzVyPUK0JI5O MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3N{C2PUc,OjR2NUewOlk9N2F-
T47D-tamR  MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HxWmlEPTB;MUmgcm0> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3N{C2PUc,OjR2NUewOlk9N2F-
293T  MVXGeY5kfGmxbjDBd5NigQ>? NGq3e3UzOCCwTR?= NYXhbVd7OyCq NFTLd45lcXO{dYD0d{BCc3RvbXXkbYF1\WRiVFnGMWlCKHO2YXLpcIl1gSxidILhcpNtd2OjdHnvckwh[W6mIHHjeIl3cXS7 Mn;lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NkO0OFkoRjJ2M{[zOFQ6RC:jPh?=
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Hep3B  NWnsdnhET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV[1MVExOCCwTR?= MWGwMVI1KGh? MoLoSG1UVw>? MWLpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> NH3ofY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5O|MxOCd-MkSyPVc{ODB:L3G+
Huh7  NVrEcmdlTnWwY4Tpc44hSXO|YYm= NV65VWI5OTByIH7N Mki4NVIhcA>? NYDPeJNYTE2VTx?= MVXpcoR2[2W|IHH1eI9xcGGpedMg NVfwfJc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVc{ODBpPkK0Nlk4OzByPD;hQi=>
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Hep-2 NV\sXWx{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjmUGIxNjBzLUGg{txO NWK3PItROTJvOU[gbC=> MkSybY5kemWjc3XzJINmdGxiZHXheIghcW5iYTDjc45k\W62cnH0bY9vNSCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVz NVzub2ZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNFM4PDlpPkK0NVA{PzR7PD;hQi=>
SCC-9 M2D5SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1e1eFAvODFvMTFOwG0> NWKwZW5rOTJvOU[gbC=> NEHZcGFqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NWPx[nE5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNFM4PDlpPkK0NVA{PzR7PD;hQi=>
Hep-2 NI\GZZlHfW6ldHnvckBCe3OjeR?= NITN[oIxNjFxMD61JO69VQ>? M1e2dlI1KGh? M2XSeIJtd2OtczDtWG9TSzFiYX7kJI1VV1KFMjDhZ5RqfmG2aX;u M1v4V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUCzO|Q6Lz5{NEGwN|c1QTxxYU6=
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CHLA-10 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\jTWM2OD1|Lkmgcm0> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
CHLA-258 NFqzbYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\mPFZSUUN3ME2xNE41KG6P M13QVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO|Y4QTxxYU6=
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NB-1643 NUTB[mdQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fm[WlEPTB;M{OuPEBvVQ>? MkfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{e2O|koRjJzM{O3Olc6RC:jPh?=
NB-EBc1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrNTWM2OD1{Nz6xJI5O NYjCSG1iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|c3PzlpPkKxN|M4Pjd7PD;hQi=>
CHLA-90 M{jNSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4m1bGlEPTB;M{SuOUBvVQ>? NGWzOZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
CHLA-136 NVT6[Go3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrLO4xiUUN3ME2xOlIvPiCwTR?= NEHzVZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
NALM-6 NFX0[nRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XHe2lEPTB;M{KuPUBvVQ>? MmrJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{e2O|koRjJzM{O3Olc6RC:jPh?=
COG-LL-317 M3rDfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV22UWdyUUN3ME2xMlMhdk1? NIrV[Is9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
RS4;11 MlnPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TOS2lEPTB;M{Wgcm0> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
MOLT-4 M1naU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFn5NGhKSzVyPUiuOkBvVQ>? MoPEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{e2O|koRjJzM{O3Olc6RC:jPh?=
CCRF-CEM MnPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTRwMTDuUS=> MlzoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{e2O|koRjJzM{O3Olc6RC:jPh?=
Kasumi-1 NXzZSJRwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3NTWM2OD1zLkSgcm0> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
Karpas-299 NUj0WHdLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLkeVhnUUN3ME2yNU44KG6P M2K1clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO|Y4QTxxYU6=
Ramos-RA1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITsdJVKSzVyPUiuNkBvVQ>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
H838 M{WxSGZ2dmO2aX;uJGF{e2G7 MXuxJO69VQ>? NWXnTVRpPDhiaB?= NGr4cIxqdmS3Y3XzJINmdGxiYYX0c5Bp[We7 NImzZVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEO2OFEyOyd-MkCzOlQyOTN:L3G+
H838 M2fSfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFWwbYYxNTNizszN NWTGU5ZiPzJiaB?= NYrSR|dEcW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 NXy1bHV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwNlg5PTRpPkKwNFI5QDV2PD;hQi=>
A549  NFfTXFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVewMVMh|ryP M4j5c|czKGh? NXq4Npc5cW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 NUj4e3MyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwNlg5PTRpPkKwNFI5QDV2PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; 

PubMed: 24457069     


Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙

p-EGFR / EGFR; 

PubMed: 25654224     


PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot;

p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; 

PubMed: 25654224     


PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.

24457069 25654224
Immunofluorescence
p-4EBP1 / EIF4E / mTOR; 

PubMed: 24600487     


(C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋€𢡄�෋€䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemセ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒セ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wセ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v 玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴￿￿嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗

LC3B / p62 ; 

PubMed: 30337536     


Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞

24600487 30337536
Growth inhibition assay
Cell viability ; 

PubMed: 29263324     


Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.

29263324
In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
- Collapse

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
- Collapse
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
- Collapse
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A
Smiles CC1COCCN1C2=NC(=NC3=C2C=CC(=N3)C4=CC(=C(C=C4)OC)CO)N5CCOCC5C

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID