AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 38 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

  • HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

    Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017, 15(1):9. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTBwMEKzN|Eh|ryP M3W2SnNCVkeHUh?=
NCI-H720 MoryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInydYJKSzVyPUCuNFI6OzhizszN MoC0V2FPT0WU
KARPAS-45 M4TVcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\GNmlEPTB;MD6wN|M4OSEQvF2= MVfTRW5ITVJ?
EW-3 MmrFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTBwMESwOVYh|ryP MYfTRW5ITVJ?
SBC-1 MnzoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHLwWWNKSzVyPUCuNFQ2QDFizszN NET5eYpUSU6JRWK=
NCI-H209 NWXDU2NyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXO4N5huUUN3ME2wMlA2PDV|IN88US=> NGiwU4NUSU6JRWK=
EoL-1-cell MkS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmriTWM2OD1yLkC2OVY6KM7:TR?= MkX1V2FPT0WU
H9 NGXZXllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{HhR2lEPTB;MD6wOlY6OiEQvF2= MYHTRW5ITVJ?
AN3-CA MkfwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvOV3JTUUN3ME2wMlA4QTVzIN88US=> NUPFO3RkW0GQR1XS
HH M1nPNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGn3eGJKSzVyPUCuNFgxODlizszN M1PCVHNCVkeHUh?=
KU812 M2raNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTv[WM6UUN3ME2wMlA5ODR|IN88US=> MVPTRW5ITVJ?
DU-4475 NF;CZ3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV7JR|UxRTBwMEiwOFch|ryP M4n6T3NCVkeHUh?=
CTV-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnEVXdKSzVyPUCuNFg3PDFizszN MXXTRW5ITVJ?
BV-173 NGDmRWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nEfWlEPTB;MD6wPVA3OSEQvF2= MnznV2FPT0WU
ES5 M3O0cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTBwMEmzOUDPxE1? MX;TRW5ITVJ?
G-402 M1X1V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXf4S3EyUUN3ME2wMlA6QThizszN Mn;EV2FPT0WU
MV-4-11 NYKye|FQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTBwMUCzOFch|ryP MkTDV2FPT0WU
NB69 NF;hT2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHhTWM2OD1yLkGzOUDPxE1? MVfTRW5ITVJ?
EGI-1 NWTmeXhUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkCxTWM2OD1yLkGzOVMyKM7:TR?= M{\abnNCVkeHUh?=
EW-18 M3LsT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoL5TWM2OD1yLkG0NFc{KM7:TR?= M2LRcXNCVkeHUh?=
ME-180 MlHWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXL3V3J4UUN3ME2wMlE1PjhzIN88US=> NYfIXW01W0GQR1XS
D-283MED M2\BOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXNVpNmUUN3ME2wMlE1PzJ|IN88US=> M13WRnNCVkeHUh?=
NB14 M{PK[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrBTWM2OD1yLkG2OFQ4KM7:TR?= NGPT[otUSU6JRWK=
Ramos-2G6-4C10 NYG5W3RrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTBwMU[5PFMh|ryP NWfC[XNVW0GQR1XS
NBsusSR NWO4co1LT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF:1OZZKSzVyPUCuNVcxOTlizszN MVTTRW5ITVJ?
LAMA-84 NWLzb5JiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPPO4hKSzVyPUCuNVgxOzJizszN NGjDO2xUSU6JRWK=
NCI-H1581 NYrtOW5NT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPsTWM2OD1yLkG4PVY3KM7:TR?= NXjjRmRpW0GQR1XS
A204 MmjPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTBwMUi5O{DPxE1? MX\TRW5ITVJ?
A549 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fIW2lEPTB;MD6xPVU2KM7:TR?= M3vDTXNCVkeHUh?=
CAL-51 NF\rZ3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTBwMUm2OVEh|ryP MUfTRW5ITVJ?
NKM-1 NX33c45[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTBwMkCwNlkh|ryP NUi3ZYdwW0GQR1XS
LoVo NHXFU2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTBwMkCyNFIh|ryP NFnYdI1USU6JRWK=
HAL-01 NGe0XGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTBwMkCyPVch|ryP M1m2WHNCVkeHUh?=
HCC2218 MnS0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnwTWM2OD1yLkKwOlg5KM7:TR?= MkDaV2FPT0WU
BEN MkjFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTBwMkGyPVMh|ryP M4rUfnNCVkeHUh?=
LC-2-ad MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3:yXWlEPTB;MD6yNlA5PCEQvF2= MV3TRW5ITVJ?
LXF-289 NWDVeYRpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\6fGlEPTB;MD6yOVA1OSEQvF2= NY\acZNrW0GQR1XS
HGC-27 NWTVbIpYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTBwMk[0NVQh|ryP MV7TRW5ITVJ?
LK-2 Ml\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn21TWM2OD1yLkK2PFI1KM7:TR?= MorOV2FPT0WU
CTB-1 M{frUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYf2PI9zUUN3ME2wMlI4OjF3IN88US=> NEDHTJpUSU6JRWK=
697 NV3leZhCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\KTWM2OD1yLkK3N|Y4KM7:TR?= MYHTRW5ITVJ?
ES7 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTBwMkezPFMh|ryP M{Xi[XNCVkeHUh?=
MG-63 NV7ZOpF3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTBwMke0NFch|ryP MWjTRW5ITVJ?
T47D MmTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfuTWM2OD1yLkK3OFc2KM7:TR?= MWjTRW5ITVJ?
RPMI-8226 NE\JVoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTBwMki1PFkh|ryP MYTTRW5ITVJ?
NCI-H292 MkjXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHLiUo9KSzVyPUCuNlkyPjRizszN MY\TRW5ITVJ?
HT NWXtUIRZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTBwMkmzPEDPxE1? MlTqV2FPT0WU
A3-KAW NYLsfVRRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rxXWlEPTB;MD6yPVc1KM7:TR?= NEfPcXBUSU6JRWK=
LB2241-RCC MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HGb2lEPTB;MD6zNFE6QCEQvF2= M1fFd3NCVkeHUh?=
BB65-RCC Mlr1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDYTWM2OD1yLkOwPFA2KM7:TR?= MUXTRW5ITVJ?
G-401 NHu5fWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfOeY1wUUN3ME2wMlMxQTh4IN88US=> M{LmOHNCVkeHUh?=
TYK-nu NEDxbIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvn[4w6UUN3ME2wMlMyQDZ2IN88US=> NEDTc|hUSU6JRWK=
NCI-SNU-1 NFrFTmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWT3eWR{UUN3ME2wMlMzOzNzIN88US=> NVvyWY5kW0GQR1XS
CHL-1 M{PJZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTQTWM2OD1yLkOyO|A{KM7:TR?= NVvzdGxHW0GQR1XS
P30-OHK MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LhPWlEPTB;MD6zNlcxPSEQvF2= Mnf3V2FPT0WU
PA-1 M33HSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jHcmlEPTB;MD6zN|YyQSEQvF2= NVPBOHdiW0GQR1XS
LB1047-RCC MknCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXITWM2OD1yLkO0NFQ4KM7:TR?= MYLTRW5ITVJ?
EM-2 M1\xd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nMSWlEPTB;MD6zOFE5QCEQvF2= MUPTRW5ITVJ?
VA-ES-BJ NIjBU5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\5UHBFUUN3ME2wMlM1OjZ6IN88US=> NGTnfYdUSU6JRWK=
NCI-H520 Mn7RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEKxU|ZKSzVyPUCuN|Q5OzRizszN M2fhVHNCVkeHUh?=
ECC10 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTBwM{WwN|Uh|ryP NX7MUYp7W0GQR1XS
LNCaP-Clone-FGC M17SRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\TTWM2OD1yLkO1N|E1KM7:TR?= NWXvb3ZIW0GQR1XS
ES8 NHfzTllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXX2TnhwUUN3ME2wMlM3PTV|IN88US=> MVfTRW5ITVJ?
HuO-3N1 MlPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnYb2FKSzVyPUCuN|g{PzlizszN MkXCV2FPT0WU
L-363 MkX5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7QTWM2OD1yLkO5NFI3KM7:TR?= NF73NFNUSU6JRWK=
SK-LU-1 NXrDTYZtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTBwM{myNFgh|ryP NHf2cG9USU6JRWK=
MCF7 NEewWVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrS[pVmUUN3ME2wMlQxPTNizszN NET4bGFUSU6JRWK=
HuO9 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7qbJJDUUN3ME2wMlQxPjZ5IN88US=> NEWxWFlUSU6JRWK=
BHT-101 M13QOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIf4U4tKSzVyPUCuOFA4QDdizszN NF\1UXFUSU6JRWK=
MZ2-MEL MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTBwNEC4PFIh|ryP NWLPcJJOW0GQR1XS
NOS-1 M1jWWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojoTWM2OD1yLkSxNFc1KM7:TR?= NFy5T21USU6JRWK=
NEC8 NHTxfWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTvTWM2OD1yLkSxOlk2KM7:TR?= NXTlTlA3W0GQR1XS
COR-L88 NVmwZZo4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTBwNEKyOlch|ryP MWDTRW5ITVJ?
MFM-223 NEK3TZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXlOHJwUUN3ME2wMlQzPjF5IN88US=> NInWSYVUSU6JRWK=
HSC-3 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn36TWM2OD1yLkSyPVA5KM7:TR?= NUXE[md{W0GQR1XS
OVCAR-5 NXTNWXFDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3yyUWlEPTB;MD60Nlk{OyEQvF2= MkPaV2FPT0WU
MFE-280 M4\iOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTBwNEOwN|Ih|ryP MYHTRW5ITVJ?
KP-N-YS M4\O[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTBwNEOwO{DPxE1? M1nKXnNCVkeHUh?=
EPLC-272H NEfLSnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1r0XWlEPTB;MD60N|A4PyEQvF2= M2i0dnNCVkeHUh?=
KE-37 NIK2c4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHPVXZ1UUN3ME2wMlQ{OjR5IN88US=> MUjTRW5ITVJ?
HuH-7 NXHOWFNqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37ZVmlEPTB;MD60N|M4OiEQvF2= M1v1WHNCVkeHUh?=
CHP-212 MkHES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwNEO2PVch|ryP MWnTRW5ITVJ?
SK-MES-1 NW\FRmVMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\vTWM2OD1yLkSzPFQ5KM7:TR?= NFmxfW5USU6JRWK=
SJSA-1 MmPES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTBwNES1OVQh|ryP NVz5endyW0GQR1XS
QIMR-WIL NYfEe4ZGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLYRYJKSzVyPUCuOFUyPSEQvF2= NIfhb4JUSU6JRWK=
ES4 MlnjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVL1ZZN[UUN3ME2wMlQ2OzN4IN88US=> NGDXdnlUSU6JRWK=
TI-73 NEjZO2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTBwNEW2PFYh|ryP M4DqXnNCVkeHUh?=
COLO-684 NHH2fFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTBwNEW3NlYh|ryP MVzTRW5ITVJ?
GCIY NFnPT4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\aSmlEPTB;MD60OVc2PCEQvF2= NFnZNmhUSU6JRWK=
JVM-3 NYHXZZJFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkTaTWM2OD1yLkS2NlkzKM7:TR?= MnXwV2FPT0WU
MPP-89 NUX1RZhvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml71TWM2OD1yLkS2PVQ4KM7:TR?= NI\JUlJUSU6JRWK=
M14 NEj6U3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnpU41kUUN3ME2wMlQ4OTdizszN M4DxZnNCVkeHUh?=
SW962 NEPJVYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnntTWM2OD1yLkS3NVkh|ryP NYjxd3ZWW0GQR1XS
VM-CUB-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLITWM2OD1yLkS3Nlg3KM7:TR?= NHqwVFdUSU6JRWK=
A101D MlHCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4q2NWlEPTB;MD60PFk3QCEQvF2= MX;TRW5ITVJ?
DB MlvqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTBwNUCwNlEh|ryP MXHTRW5ITVJ?
KNS-62 NFjmW29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXn0T25uUUN3ME2wMlUxPTZ6IN88US=> M1XYW3NCVkeHUh?=
NCI-H460 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLwPIxlUUN3ME2wMlUxPTl5IN88US=> Mmq0V2FPT0WU
MDA-MB-361 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M12xWWlEPTB;MD61NFg4KM7:TR?= M1;DcnNCVkeHUh?=
DOK MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPuXmVDUUN3ME2wMlUyOzN|IN88US=> MlvZV2FPT0WU
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Huh7  MXzGeY5kfGmxbjDBd5NigQ>? MmO4NVAxKG6P NFLYUJgyOiCq MYfEUXNQ NH;CTmtqdmS3Y3XzJIF2fG:yaHHnfeKh Mn7sNlQzQTd|MEC=
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Rh18 NIrR[FJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4i4dmlEPTB;NEGuOEBvVQ>? MVGyNVM{PzZ5OR?=
Rh30 NGmwO5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLrNIhEUUN3ME2xOEBvVQ>? MWqyNVM{PzZ5OR?=
BT-12 MlH2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvPWXE1UUN3ME2yOE44KG6P NHLm[XAzOTN|N{[3PS=>
CHLA-266 M1G3WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYK1bnBQUUN3ME2xMlEhdk1? NWHi[nd{OjF|M{e2O|k>
TC-71 NGjXd3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDCSJJKSzVyPUS2MlUhdk1? M3Lud|IyOzN5Nke5
CHLA-9 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnX3TWM2OD1zNT6zJI5O NHzQbWszOTN|N{[3PS=>
CHLA-10 MmXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI[wS2xKSzVyPUOuPUBvVQ>? NYjyc4xCOjF|M{e2O|k>
CHLA-258 MmrqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\BNGlEPTB;MUCuOEBvVQ>? M2DkbVIyOzN5Nke5
GBM2 M{L4Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTpO2x{UUN3ME20NE44KG6P M1PpTFIyOzN5Nke5
NB-1643 M{jsV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4X1RmlEPTB;M{OuPEBvVQ>? NGHPWVUzOTN|N{[3PS=>
NB-EBc1 M4HrbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHLTWM2OD1{Nz6xJI5O MmPENlE{Ozd4N{m=
CHLA-90 Mmq2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGrwVYFKSzVyPUO0MlUhdk1? NUfMXYNUOjF|M{e2O|k>
CHLA-136 M3TBb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jxWmlEPTB;MU[yMlYhdk1? M2PTPVIyOzN5Nke5
NALM-6 NYrLbWhQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrxZmo2UUN3ME2zNk46KG6P MX:yNVM{PzZ5OR?=
COG-LL-317 NXf2ZZJNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTFwMzDuUS=> MUeyNVM{PzZ5OR?=
RS4;11 MmfnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3r6OGlEPTB;M{Wgcm0> M2LRfVIyOzN5Nke5
MOLT-4 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DxbmlEPTB;OD62JI5O NETiZlkzOTN|N{[3PS=>
CCRF-CEM NVjFNVlST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXe5Xlh1UUN3ME20MlEhdk1? MWSyNVM{PzZ5OR?=
Kasumi-1 NVH0[pF6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3T3NGlEPTB;MT60JI5O M2D4[FIyOzN5Nke5
Karpas-299 M2XubGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{S2NGlEPTB;MkGuO{BvVQ>? MlHwNlE{Ozd4N{m=
Ramos-RA1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTITWM2OD16LkKgcm0> MVWyNVM{PzZ5OR?=
H838 NH3IVoxHfW6ldHnvckBCe3OjeR?= M1nsTFEh|ryP NWf4UIVtPDhiaB?= NXXnU3RRcW6mdXPld{Bk\WyuIHH1eI9xcGGpeR?= Ml;pNlA{PjRzMUO=
H838 NYHPdYZLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PkXVAuOyEQvF2= NFH6W4o4OiCq MmHwbY5lfWOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh[XW2b4DoZYd6 Ml7XNlAxOjh6NUS=
A549  NW\GO2I2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHSNE0{KM7:TR?= NV:3eYpOPzJiaB?= NUjXb2ExcW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 Ml2yNlAxOjh6NUS=

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID