AZD8055

Catalog No.S1555

For research use only.

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.

AZD8055 Chemical Structure

CAS No. 1009298-09-2

Selleck's AZD8055 has been cited by 228 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Other mTOR Products

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 NYH1NopGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTBwMEKzN|Eh|ryP NYHpR|VbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
NCI-H720 M{XTbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTBwMEK5N|gh|ryP M4XCOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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SBC-1 M33scGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTBwMES1PFEh|ryP MojWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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EoL-1-cell MkWwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHtTIVKSzVyPUCuNFY2PjlizszN MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
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LB2241-RCC MoW2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTBwM{CxPVgh|ryP NWrqc2VjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
BB65-RCC MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvWZm5KSzVyPUCuN|A5ODVizszN NW\NXIV4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
G-401 NF7sS3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTBwM{C5PFYh|ryP MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
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LB1047-RCC NHvkUZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVr4SHEyUUN3ME2wMlM1ODR5IN88US=> NHXyUGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
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VA-ES-BJ NGPZZotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vNPGlEPTB;MD6zOFI3QCEQvF2= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
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ECC10 NFn3[FZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXoT|BKSzVyPUCuN|UxOzVizszN NY\1c5ZORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
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L-363 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfod|k2UUN3ME2wMlM6ODJ4IN88US=> NUGyboVYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
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MCF7 NVHIPJUyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPGTWM2OD1yLkSwOVMh|ryP NULRWoc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
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COR-L88 M2\kTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HIOGlEPTB;MD60NlI3PyEQvF2= M{G3VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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HSC-3 NF65SYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDxTWM2OD1yLkSyPVA5KM7:TR?= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
OVCAR-5 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmD3TWM2OD1yLkSyPVM{KM7:TR?= Mo\0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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KP-N-YS NGfrVWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7WTGxKSzVyPUCuOFMxPyEQvF2= NIq3d5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
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HuH-7 NUjPUmxvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LRZ2lEPTB;MD60N|M4OiEQvF2= NWLqTpV5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
CHP-212 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnX6TWM2OD1yLkSzOlk4KM7:TR?= NES1WnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
SK-MES-1 M1;TV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH3RVYtKSzVyPUCuOFM5PDhizszN Ml\FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
SJSA-1 NHjCdHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknxTWM2OD1yLkS0OVU1KM7:TR?= NUjYemhLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
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TI-73 NFPmeWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLwTWM2OD1yLkS1Olg3KM7:TR?= MnmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
COLO-684 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnKdW5KSzVyPUCuOFU4OjZizszN M1PTS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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MPP-89 NEjyNWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPETWM2OD1yLkS2PVQ4KM7:TR?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
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COLO-824 NVLSOodwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHjVmNrUUN3ME2yNE46Ojd4IN88US=> MmTLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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KALS-1 Mn70S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWraTGNGUUN3ME2yNU4yPzVizszN NGS5[Y09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
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JAR MkfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLIeJBoUUN3ME2yNU42ODJizszN MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
DoTc2-4510 NFe4W3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jhcWlEPTB;MkOuPFQ4PSEQvF2= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
ABC-1 NF[3V|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jpNGlEPTB;Mk[uO|k2PyEQvF2= NGDvS4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
MHH-NB-11 NGniWZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\MOGlEPTB;M{OuN|Q3PCEQvF2= M{nqV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
SW1463 NEjCe3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXvTWM2OD1|Mz65PFMh|ryP NGW3fnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
SW1417 NE\FZVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmn3TWM2OD1|Nz6wPVUzKM7:TR?= MlfBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
NB12 MmG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVuxcpFHUUN3ME2zPU41PjV3IN88US=> Mn;zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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H1048 NUjCPXVySXCxcITvd4l{KEG|c3H5 MVm1NFAhdk1? NIDCU|U4OiCq MoTNcohidmOnczDBRnQuOjZ|4pETbY5lfWOnZDDhdI9xfG:|aYO= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd|N{W0Nkc,OjV5M{e1OFI9N2F-
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H446 NVq5VlJpSXCxcITvd4l{KEG|c3H5 NFvN[Yo2ODBibl2= M1KxRlczKGh? MX3ubIFv[2W|IFHCWE0zPjQkgKPpcoR2[2WmIHHwc5B1d3Orcx?= NVvCSotTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3N|c2PDJpPkK1O|M4PTR{PD;hQi=>
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T47D-tamR  NHXSOGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTF7IH7N NUP2WINsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVcxPjlpPkK0OFU4ODZ7PD;hQi=>
293T  NYeyfXVbTnWwY4Tpc44hSXO|YYm= NGfyXZYzOCCwTR?= NFz0Rng{KGh? NVm5dZFq\Gm|coXweJMhSWu2LX3l[IlifGWmIGTJSk1KSSC|dHHibYxqfHluIITyZY5{dG:lYYTpc44tKGGwZDDhZ5Rqfmm2eR?= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4M{S0PUc,OjR|NkO0OFk9N2F-
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Huh7  NWHBRXhpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHH1dpA2NTFyMDDuUS=> MnS0NE0zPCCq M2PEdWROW09? NYH0Vm8zcW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NX:5bYs{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVc{ODBpPkK0Nlk4OzByPD;hQi=>
Hep3B  NXrGWFh1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LD[lUuOTByIH7N MkL6NE0zPCCq NVz0fXM5TE2VTx?= NWLRUYdxcW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NVzrW3VpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVc{ODBpPkK0Nlk4OzByPD;hQi=>
Huh7  NYj0PWpCTnWwY4Tpc44hSXO|YYm= MUexNFAhdk1? MUWxNkBp NYTmbXJITE2VTx?= NYDhVYFLcW6mdXPld{BifXSxcHjh[5nDqA>? NXnnfWw{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVc{ODBpPkK0Nlk4OzByPD;hQi=>
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 L3.6pl NYH5OWJvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUWxJO69VQ>? MUS3NkBp M3zNN4lvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDjc41jcW6nZDD3bZRpKEKPUz23O|c3ODd? M2LIZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkOzN|k6Lz5{NEKzN|M6QTxxYU6=
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RD MorkRZBweHSxc3nzJGF{e2G7 NEnCT2syKM7:TR?= NEnmcJc1QCCq M{\JUYlv[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JGFDXC15M{e= NWHLZ4M6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN|MzOThpPkK0NVM{OjF6PD;hQi=>
RMS13 M3fVSmFxd3C2b4Ppd{BCe3OjeR?= M3PHeVEh|ryP MnL3OFghcA>? M1HoWYlv[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JGFDXC15M{e= NEXZemY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGzN|IyQCd-MkSxN|MzOTh:L3G+
RH30 MX\BdI9xfG:|aYOgRZN{[Xl? NF76e5cyKM7:TR?= M2Xs[VQ5KGh? NHXsc2NqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBCSlRvN{O3 M4S0clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUOzNlE5Lz5{NEGzN|IyQDxxYU6=
Hep-2 NH7yflRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml75NE4xOS1zIN88US=> M1X2SlEzNTl4IHi= NEeyUo5qdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NVjYZVFIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNFM4PDlpPkK0NVA{PzR7PD;hQi=>
SCC-9 NVqzVXF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYr1NXNUOC5yMT2xJO69VQ>? M4jt[VEzNTl4IHi= NEDsWpVqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NGj2OWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGwN|c1QSd-MkSxNFM4PDl:L3G+
Hep-2 MW\GeY5kfGmxbjDBd5NigQ>? MXWwMlEwOC53IN88US=> MnLTNlQhcA>? MkDuZoxw[2u|IH3UU3JEOSCjbnSgcXRQWkN{IHHjeIl3[XSrb36= MlLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMEO3OFkoRjJ2MUCzO|Q6RC:jPh?=
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CHLA-266 NFG4SVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTFwMTDuUS=> NGTSbHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
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CHLA-9 NWHB[GN[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTF3LkOgcm0> M2GyblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO|Y4QTxxYU6=
CHLA-10 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M32zVGlEPTB;Mz65JI5O MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
CHLA-258 NH7UfFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTlPY5rUUN3ME2xNE41KG6P M4ix[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO|Y4QTxxYU6=
GBM2 NETrSmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7mTWM2OD12MD63JI5O NFi1NVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
NB-1643 NWjNT|dbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTN|Lkigcm0> NV;IZ|JqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|c3PzlpPkKxN|M4Pjd7PD;hQi=>
NB-EBc1 NFHFPIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTJ5LkGgcm0> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
CHLA-90 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3MOmVIUUN3ME2zOE42KG6P MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
CHLA-136 M1vtTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTF4Mj62JI5O NIOwNpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
NALM-6 M2TFd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTMNotKSzVyPUOyMlkhdk1? NYf0dXhoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|c3PzlpPkKxN|M4Pjd7PD;hQi=>
COG-LL-317 NH7UNYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NET6b25KSzVyPUGuN{BvVQ>? Mn3SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{e2O|koRjJzM{O3Olc6RC:jPh?=
RS4;11 MmmzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnEV|FKSzVyPUO1JI5O M1\MN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO|Y4QTxxYU6=
MOLT-4 MlTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRThwNjDuUS=> MkfpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{e2O|koRjJzM{O3Olc6RC:jPh?=
CCRF-CEM NVWwTopQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTRwMTDuUS=> NXiyXJB6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|c3PzlpPkKxN|M4Pjd7PD;hQi=>
Kasumi-1 M3jDemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULsSodxUUN3ME2xMlQhdk1? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
Karpas-299 NHnqTW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzrXmhmUUN3ME2yNU44KG6P MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
Ramos-RA1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRThwMjDuUS=> NFrOWYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
H838 NUDDUGd4TnWwY4Tpc44hSXO|YYm= NG\oUpcyKM7:TR?= M2TI[|Q5KGh? MlXHbY5lfWOnczDj[YxtKGG3dH;wbIFogQ>? MmSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|NkSxNVMoRjJyM{[0NVE{RC:jPh?=
H838 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDYNE0{KM7:TR?= NWjnbmRoPzJiaB?= NIj1N|dqdmS3Y3XzJIdzd3e2aDDpcohq[mm2aX;uJIFv\CCjdYTvdIhi\3l? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB{OEi1OEc,OjByMki4OVQ9N2F-
A549  MnfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVuwMVMh|ryP Mm\tO|IhcA>? MYHpcoR2[2W|IHfyc5d1cCCrbnjpZol1cW:wIHHu[EBifXSxcHjh[5k> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB{OEi1OEc,OjByMki4OVQ9N2F-
Assay
Methods Test Index PMID
Western blot p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40 ; p-EGFR / EGFR ; p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a 24457069 25654224
Immunofluorescence p-4EBP1 / EIF4E / mTOR ; LC3B / p62 24600487 30337536
Growth inhibition assay Cell viability 29263324
In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol (from reference)

Kinase Assay:[1]
  • Cell-based determinations of mTOR inhibition:

    A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.

Cell Research:[1]
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
Animal Research:[1]
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1COCCN1C2=NC(=NC3=C2C=CC(=N3)C4=CC(=C(C=C4)OC)CO)N5CCOCC5C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Drug: AZD8055 Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT00999882 Completed Drug: AZD8055 Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Drug: AZD8055 Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Drug: AZD8055 Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Drug: AZD8055 Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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