AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
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Cited by 70 Publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MnLRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\hPGlEPTB;MD6wNlM{OSEQvF2= M2DZ[3NCVkeHUh?=
NCI-H720 MkT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTsTWM2OD1yLkCyPVM5KM7:TR?= MXfTRW5ITVJ?
KARPAS-45 MoHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXuTWM2OD1yLkCzN|cyKM7:TR?= NV\GVlh4W0GQR1XS
EW-3 M3n3NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn[1TWM2OD1yLkC0NFU3KM7:TR?= MXzTRW5ITVJ?
SBC-1 NV7J[nk6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LvO2lEPTB;MD6wOFU5OSEQvF2= MnryV2FPT0WU
NCI-H209 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETpVVlKSzVyPUCuNFU1PTNizszN M1\hdHNCVkeHUh?=
EoL-1-cell NGDOTYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTBwME[1Olkh|ryP NE[3XIpUSU6JRWK=
H9 Mlf6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTBwME[2PVIh|ryP NUTuXnhOW0GQR1XS
AN3-CA MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITLellKSzVyPUCuNFc6PTFizszN NHvUfVZUSU6JRWK=
HH MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXyOJBqUUN3ME2wMlA5ODB7IN88US=> NXrlVFVHW0GQR1XS
KU812 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXaTWM2OD1yLkC4NFQ{KM7:TR?= NXzORolEW0GQR1XS
DU-4475 M1vwfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;VNmlEPTB;MD6wPFA1PyEQvF2= Mn;nV2FPT0WU
CTV-1 NHTnZ4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnK5TWM2OD1yLkC4OlQyKM7:TR?= M{\tNHNCVkeHUh?=
BV-173 MmnNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTBwMEmwOlEh|ryP NHHH[4dUSU6JRWK=
ES5 M3vkOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX23VVkyUUN3ME2wMlA6OzVizszN M2PUVHNCVkeHUh?=
G-402 NUXFdJMzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vJWWlEPTB;MD6wPVk5KM7:TR?= MmnIV2FPT0WU
MV-4-11 NEiyfZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1yzW2lEPTB;MD6xNFM1PyEQvF2= NGDKb45USU6JRWK=
NB69 Mo\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rETGlEPTB;MD6xN|Uh|ryP MYDTRW5ITVJ?
EGI-1 NX:0V5l1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDTZWM{UUN3ME2wMlE{PTNzIN88US=> NYrLVI5vW0GQR1XS
EW-18 NVLvcolHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMUSwO|Mh|ryP NWfM[|BQW0GQR1XS
ME-180 NY\5VXZVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTBwMUS2PFEh|ryP MVPTRW5ITVJ?
D-283MED MoHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7YTWM2OD1yLkG0O|I{KM7:TR?= MmX5V2FPT0WU
NB14 NGn4N3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXoTWM2OD1yLkG2OFQ4KM7:TR?= M2XOfnNCVkeHUh?=
Ramos-2G6-4C10 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjodoV[UUN3ME2wMlE3QTh|IN88US=> MlXzV2FPT0WU
NBsusSR MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTBwMUewNVkh|ryP MmfUV2FPT0WU
LAMA-84 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHTTWM2OD1yLkG4NFMzKM7:TR?= NFnZU|RUSU6JRWK=
NCI-H1581 MkK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzLTWM2OD1yLkG4PVY3KM7:TR?= NWH5fHdLW0GQR1XS
A204 NXz1SXpFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjjNoROUUN3ME2wMlE5QTdizszN M1vzenNCVkeHUh?=
A549 NYPVRpdIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fiR2lEPTB;MD6xPVU2KM7:TR?= Mn;WV2FPT0WU
CAL-51 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTRS3BKSzVyPUCuNVk3PTFizszN NV[5c|hDW0GQR1XS
NKM-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXwOZNyUUN3ME2wMlIxODJ7IN88US=> NWTtOY1IW0GQR1XS
LoVo M{\KfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mly5TWM2OD1yLkKwNlAzKM7:TR?= MYDTRW5ITVJ?
HAL-01 MlTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2OyXWlEPTB;MD6yNFI6PyEQvF2= M1WyfXNCVkeHUh?=
HCC2218 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3y2U2lEPTB;MD6yNFY5QCEQvF2= M{L3OHNCVkeHUh?=
BEN M3fwUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnRe2xsUUN3ME2wMlIyOjl|IN88US=> MmfjV2FPT0WU
LC-2-ad NELDbHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrYe5h4UUN3ME2wMlIzODh2IN88US=> NU[4PIV{W0GQR1XS
LXF-289 NUjWS5NoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4i5VWlEPTB;MD6yOVA1OSEQvF2= MnnRV2FPT0WU
HGC-27 NYLBfY9TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjQTWM2OD1yLkK2OFE1KM7:TR?= MXvTRW5ITVJ?
LK-2 NGX0PW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnG2TWM2OD1yLkK2PFI1KM7:TR?= NYHiblViW0GQR1XS
CTB-1 MnzOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnm4TWM2OD1yLkK3NlE2KM7:TR?= Mk\2V2FPT0WU
697 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3EXm1[UUN3ME2wMlI4OzZ5IN88US=> M1P4fXNCVkeHUh?=
ES7 M2HBeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1rjRWlEPTB;MD6yO|M5OyEQvF2= M1TucnNCVkeHUh?=
MG-63 MkLjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTBwMke0NFch|ryP NGLzXWxUSU6JRWK=
T47D MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEj1RoVKSzVyPUCuNlc1PzVizszN MVjTRW5ITVJ?
RPMI-8226 Ml7nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DQPWlEPTB;MD6yPFU5QSEQvF2= Mmn4V2FPT0WU
NCI-H292 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrETWM2OD1yLkK5NVY1KM7:TR?= M1zh[XNCVkeHUh?=
HT M2rKV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTHe21WUUN3ME2wMlI6OzhizszN MWHTRW5ITVJ?
A3-KAW MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXuTWM2OD1yLkK5O|Qh|ryP MkLKV2FPT0WU
LB2241-RCC NGHxdIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\XW4tKSzVyPUCuN|AyQThizszN NVfw[Ws5W0GQR1XS
BB65-RCC NF\ONYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\YRWlEPTB;MD6zNFgxPSEQvF2= NVTqO4U2W0GQR1XS
G-401 NYrEdIF6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPlO2ZKSzVyPUCuN|A6QDZizszN M4DPOHNCVkeHUh?=
TYK-nu MlvaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTBwM{G4OlQh|ryP MVzTRW5ITVJ?
NCI-SNU-1 MoDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3zS2ZKSzVyPUCuN|I{OzFizszN MnjYV2FPT0WU
CHL-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3TEUWlEPTB;MD6zNlcxOyEQvF2= NFmzdVVUSU6JRWK=
P30-OHK MlfsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTBwM{K3NFUh|ryP MYDTRW5ITVJ?
PA-1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7kTWM2OD1yLkOzOlE6KM7:TR?= MVjTRW5ITVJ?
LB1047-RCC NIW2b2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHkWZhHUUN3ME2wMlM1ODR5IN88US=> M4Xrc3NCVkeHUh?=
EM-2 NVnpSldFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWWxeJJ[UUN3ME2wMlM1OTh6IN88US=> MVXTRW5ITVJ?
VA-ES-BJ MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPYTZVKSzVyPUCuN|QzPjhizszN NX[ySpR4W0GQR1XS
NCI-H520 M4TE[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVH1bGlOUUN3ME2wMlM1QDN2IN88US=> M3jseXNCVkeHUh?=
ECC10 MlPZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTsTWM2OD1yLkO1NFM2KM7:TR?= NIPCeo5USU6JRWK=
LNCaP-Clone-FGC NGPxd|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mme4TWM2OD1yLkO1N|E1KM7:TR?= NV6yb3I3W0GQR1XS
ES8 NILESlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW[4RWFmUUN3ME2wMlM3PTV|IN88US=> MV\TRW5ITVJ?
HuO-3N1 NUO3UnQ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYD2UXN[UUN3ME2wMlM5Ozd7IN88US=> NF;PfIhUSU6JRWK=
L-363 NEL5[XpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXYVW9KSzVyPUCuN|kxOjZizszN NILM[YpUSU6JRWK=
SK-LU-1 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTBwM{myNFgh|ryP NFTvU4VUSU6JRWK=
MCF7 MkL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPXXGJ5UUN3ME2wMlQxPTNizszN MWDTRW5ITVJ?
HuO9 MnXxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnsTWM2OD1yLkSwOlY4KM7:TR?= Ml7aV2FPT0WU
BHT-101 NV\5e2MyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTBwNEC3PFch|ryP MUPTRW5ITVJ?
MZ2-MEL NILsZ2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLxTWM2OD1yLkSwPFgzKM7:TR?= M1Pk[XNCVkeHUh?=
NOS-1 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTBwNEGwO|Qh|ryP Mnu5V2FPT0WU
NEC8 M2m5Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTBwNEG2PVUh|ryP MoP3V2FPT0WU
COR-L88 Mn\hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTBwNEKyOlch|ryP NXrqdlR4W0GQR1XS
MFM-223 NGDx[WVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnH3TWM2OD1yLkSyOlE4KM7:TR?= NYfHNmgyW0GQR1XS
HSC-3 NGnPV|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTBwNEK5NFgh|ryP Mmq2V2FPT0WU
OVCAR-5 MlW3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3[2WGlEPTB;MD60Nlk{OyEQvF2= NGTlRo5USU6JRWK=
MFE-280 MonWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\2c4hKUUN3ME2wMlQ{ODN{IN88US=> NUXkNIM3W0GQR1XS
KP-N-YS MnnZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7OOXZKSzVyPUCuOFMxPyEQvF2= MX3TRW5ITVJ?
EPLC-272H MoH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLiTWM2OD1yLkSzNFc4KM7:TR?= MWDTRW5ITVJ?
KE-37 NEXrS3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHOTWM2OD1yLkSzNlQ4KM7:TR?= MULTRW5ITVJ?
HuH-7 NGT4fmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjXPW5GUUN3ME2wMlQ{Ozd{IN88US=> M4H2e3NCVkeHUh?=
CHP-212 NEexcI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVuzeFFuUUN3ME2wMlQ{Pjl5IN88US=> MX7TRW5ITVJ?
SK-MES-1 M3TtV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPDTYlyUUN3ME2wMlQ{QDR6IN88US=> MV\TRW5ITVJ?
SJSA-1 MlnJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH75W2xKSzVyPUCuOFQ2PTRizszN NULyVmhGW0GQR1XS
QIMR-WIL MojjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETkemlKSzVyPUCuOFUyPSEQvF2= NH;qRZZUSU6JRWK=
ES4 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLETWM2OD1yLkS1N|M3KM7:TR?= NXPmR4djW0GQR1XS
TI-73 MmD3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzRdlFKSzVyPUCuOFU3QDZizszN M4frW3NCVkeHUh?=
COLO-684 M3fnWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;2[mlEPTB;MD60OVczPiEQvF2= M{DYVnNCVkeHUh?=
GCIY MlTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHS2dplKSzVyPUCuOFU4PTRizszN M1jkZnNCVkeHUh?=
JVM-3 NEjX[4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHNWZR{UUN3ME2wMlQ3Ojl{IN88US=> M4jYOHNCVkeHUh?=
MPP-89 NYr0S|A4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nJWmlEPTB;MD60Olk1PyEQvF2= NFXSZWZUSU6JRWK=
M14 NXnuSlZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTBwNEexO{DPxE1? MkLiV2FPT0WU
SW962 NEPu[4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7Wc|JKSzVyPUCuOFcyQSEQvF2= NHjvNWJUSU6JRWK=
VM-CUB-1 M1Sxfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXi0SXIyUUN3ME2wMlQ4Ojh4IN88US=> NE\4ZoZUSU6JRWK=
A101D NHniTolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW[5W|NzUUN3ME2wMlQ5QTZ6IN88US=> M2\EN3NCVkeHUh?=
DB MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXLSopKSzVyPUCuOVAxOjFizszN NELyT3FUSU6JRWK=
KNS-62 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGD2dGdKSzVyPUCuOVA2PjhizszN M4jUeHNCVkeHUh?=
NCI-H460 MkTSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3jclkzUUN3ME2wMlUxPTl5IN88US=> MlzkV2FPT0WU
MDA-MB-361 NEXoUppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{W1PGlEPTB;MD61NFg4KM7:TR?= MV;TRW5ITVJ?
DOK MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLEZWVKSzVyPUCuOVE{OzNizszN NVu0U415W0GQR1XS
CAL-39 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTBwNUKyOlgh|ryP MlP5V2FPT0WU
HT-1080 NHvNW5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLXTWM2OD1yLkWyOVQ{KM7:TR?= MV\TRW5ITVJ?
GR-ST MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHBTWM2OD1yLkWzNFE2KM7:TR?= NXPmNpZUW0GQR1XS
J-RT3-T3-5 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M32ybmlEPTB;MD61N|E6PSEQvF2= NUC3TGhtW0GQR1XS
NCI-H1793 MkLMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTBwNUO0OFch|ryP NU\OcJZrW0GQR1XS
NCI-H1355 NXLrTJZqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1SzWmlEPTB;MD61N|g4QCEQvF2= MljGV2FPT0WU
YH-13 MoPjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGn5dG9KSzVyPUCuOVQ6OzhizszN M3u5WHNCVkeHUh?=
LOXIMVI MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTBwNUWxNlMh|ryP MlXqV2FPT0WU
ES1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MorkTWM2OD1yLkW1OVE5KM7:TR?= M13xenNCVkeHUh?=
GAMG NF6xZWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDaTWM2OD1yLkW1OlU5KM7:TR?= MULTRW5ITVJ?
SK-MEL-28 NFXmZ21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmmwTWM2OD1yLkW1PVEh|ryP MnLiV2FPT0WU
KG-1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTBwNU[4PFQh|ryP M13LNHNCVkeHUh?=
HOS NETpPFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETCOZNKSzVyPUCuOVcyQTJizszN MV7TRW5ITVJ?
PANC-08-13 NX3XSHY1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHMOohKSzVyPUCuOVc{QDlizszN M4e3cnNCVkeHUh?=
SW780 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTBwNUe1PVUh|ryP MnnzV2FPT0WU
MDA-MB-175-VII NEnyc5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnFTWM2OD1yLkW3O|QyKM7:TR?= NVjMbnJqW0GQR1XS
MSTO-211H MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7NTlJKSzVyPUCuOVc4PTFizszN NYHPRnRMW0GQR1XS
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COR-L23 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLBTWM2OD1zLkO0PFQ2KM7:TR?= MXjTRW5ITVJ?
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HEL MmLXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLvTWM2OD1zLkm0NVg1KM7:TR?= M1rrd3NCVkeHUh?=
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OVCAR-8 MmDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrQSoNKSzVyPUKuNFAzQTVizszN MkOwV2FPT0WU
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NCI-H1395 MnXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnsWmdKSzVyPUKuNVA6QDZizszN MlPYV2FPT0WU
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OVCAR-3 NEnCNIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4q4b2lEPTB;Mz6wNlEyPSEQvF2= NEfKbFRUSU6JRWK=
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LB771-HNC MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfCTWM2OD1|LkC5N|Q{KM7:TR?= MkG2V2FPT0WU
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LCLC-103H MkHlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWi1dY9rUUN3ME2zMlI{QDl2IN88US=> NUezXJdNW0GQR1XS
U-266 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTNwMke2N|Mh|ryP M3PWOXNCVkeHUh?=
JVM-2 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MliyTWM2OD1|LkOyNVY1KM7:TR?= MmHPV2FPT0WU
UACC-257 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjsZYRNUUN3ME2zMlQ3PDNzIN88US=> NVG2WZM6W0GQR1XS
LB996-RCC MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWexVYdXUUN3ME2zMlQ3PTB{IN88US=> M3jQT3NCVkeHUh?=
BT-549 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTNwNUCyNFkh|ryP MYXTRW5ITVJ?
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NCI-H2291 M1mydWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jmfGlEPTB;Mz63OVM{PiEQvF2= Mnu3V2FPT0WU
NCI-H441 NXzwcJRWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml25TWM2OD1|LkiyOFM{KM7:TR?= NGjYVWNUSU6JRWK=
D-247MG NYrTV2lyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\FPFV6UUN3ME2zMlg3ODNzIN88US=> M12z[nNCVkeHUh?=
COLO-320-HSR MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYT3dFVXUUN3ME2zMlkyOjdzIN88US=> Mo\NV2FPT0WU
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SF268 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPHVVlKSzVyPUSuNVI5QDFizszN NV3JWHR{W0GQR1XS
RMG-I NGDI[oxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfXTWM2OD12LkKxOFU1KM7:TR?= M{fSRXNCVkeHUh?=
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GP5d MkTZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTRwNEGyPVgh|ryP NEfKd2ZUSU6JRWK=
UACC-62 MnTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXy1Upd7UUN3ME20MlQ3PTR3IN88US=> MWXTRW5ITVJ?
MS-1 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTRwNEmxNVUh|ryP NXzhVZJiW0GQR1XS
RCM-1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PENmlEPTB;ND61NlkzKM7:TR?= MoHQV2FPT0WU
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SW756 MmnMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTnTWM2OD12Lk[5NVM2KM7:TR?= NEjUSJZUSU6JRWK=
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HCC1937 MmTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXDTWM2OD12Lki1NVI2KM7:TR?= MoTXV2FPT0WU
OAW-28 NVvHfYxqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVi1dmdPUUN3ME20MlkzPDN3IN88US=> NFLLNlJUSU6JRWK=
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GI-1 MmnqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3jTWM2OD13LkKzOFg3KM7:TR?= MYLTRW5ITVJ?
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ONS-76 M4LDfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTVwNUW4NFEh|ryP NUHGNYRzW0GQR1XS
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COLO-741 NVLZSIh7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2Oz[2lEPTB;NT63NVk6PSEQvF2= M{jWNXNCVkeHUh?=
HT-29 MlXUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPMc5FPUUN3ME21MlgxPDd3IN88US=> MULTRW5ITVJ?
HCC1187 NVjMc3lCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXVTWM2OD13LkixNFA5KM7:TR?= Ml:xV2FPT0WU
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NCI-H630 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTZwMUG0OFEh|ryP NVvBS2NtW0GQR1XS
COLO-678 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLQeZZKSzVyPU[uNVUxQTJizszN MUPTRW5ITVJ?
D-502MG NVWxcJIzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mkj3TWM2OD14LkG3N|M3KM7:TR?= NF6zU5dUSU6JRWK=
NB17 MnjBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zF[WlEPTB;Nj6yOFk1OyEQvF2= MmWyV2FPT0WU
SK-MEL-24 NIniWVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTjXJpKSzVyPU[uNlY1QSEQvF2= MVPTRW5ITVJ?
NCI-SNU-5 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzDVW1KSzVyPU[uNlcyOThizszN NVfYR2lZW0GQR1XS
D-392MG MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4X5dGlEPTB;Nj6zN|M6QSEQvF2= NG\hbJBUSU6JRWK=
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CHLA-136 M2rKbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEjn[GJKSzVyPUG2Nk43KG6P MkSwNlE{Ozd4N{m=
NALM-6 NF7DdWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TnOWlEPTB;M{KuPUBvVQ>? MUiyNVM{PzZ5OR?=
COG-LL-317 NIfvdIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDndmF1UUN3ME2xMlMhdk1? M2TV[FIyOzN5Nke5
RS4;11 MlPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknxTWM2OD1|NTDuUS=> NUi3PFJIOjF|M{e2O|k>
MOLT-4 NGXKWpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PsdWlEPTB;OD62JI5O MYWyNVM{PzZ5OR?=
CCRF-CEM NXfwPJNXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mny5TWM2OD12LkGgcm0> MWGyNVM{PzZ5OR?=
Kasumi-1 Mn3KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTFwNDDuUS=> NIXGZ|kzOTN|N{[3PS=>
Karpas-299 M2jCbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTJzLkegcm0> NF:4[JczOTN|N{[3PS=>
Ramos-RA1 M2rKXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXaNnRnUUN3ME24MlIhdk1? MmDLNlE{Ozd4N{m=
H838 MX3GeY5kfGmxbjDBd5NigQ>? NXrZXWJwOSEQvF2= NVrIV3ZFPDhiaB?= NGr6RnFqdmS3Y3XzJINmdGxiYYX0c5Bp[We7 Mk\BNlA{PjRzMUO=
H838 NVPaOnFvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlzyNE0{KM7:TR?= NGmwVG44OiCq NEXMfFdqdmS3Y3XzJIdzd3e2aDDpcohq[mm2aX;uJIFv\CCjdYTvdIhi\3l? NXXMUIFLOjByMki4OVQ>
A549  NVHwfHJ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofMNE0{KM7:TR?= MVu3NkBp Mlf6bY5lfWOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh[XW2b4DoZYd6 M2nuSlIxODJ6OEW0

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; 

PubMed: 24457069     


Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙

p-EGFR / EGFR; 

PubMed: 25654224     


PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot;

p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; 

PubMed: 25654224     


PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.

24457069 25654224
Immunofluorescence
p-4EBP1 / EIF4E / mTOR; 

PubMed: 24600487     


(C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋€𢡄�෋€䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemセ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒セ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wセ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v 玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴￿￿嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗

LC3B / p62 ; 

PubMed: 30337536     


Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞

24600487 30337536
Growth inhibition assay
Cell viability ; 

PubMed: 29263324     


Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.

29263324
In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID