AZD8055

Name: AZD8055
Brand: Selleck Chemicals
SKU: S1555
Rating: 5 (198 reviews)

For research use only.

Catalog No.S1555

198 publications

CAS No. 1009298-09-2

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.

Selleck's AZD8055 has been cited by 198 publications

Nature, 2021, 591(7849):288-292

PubMed: 33658715 ( click the link to review the publication )

Immunity, 2021, S1074-7613(21)00069-8

PubMed: 33667382 ( click the link to review the publication )

Nature, 2019, 10.1038/s41586-019-1607-3

PubMed: 31578521 ( click the link to review the publication )

Nat Med, 2019, 25(5):838-849

PubMed: 31011202 ( click the link to review the publication )

Cancer Cell, 2019, 35(5):752-766

PubMed: 31085176 ( click the link to review the publication )

Cell, 2018, 172(1-2):373-386

PubMed: 29224780 ( click the link to review the publication )

Cell Stem Cell, 2018, 22(3):369-383

PubMed: 29499153 ( click the link to review the publication )

Nat Med, 2017, 23(2):200-212

PubMed: 28067899 ( click the link to review the publication )

Cell Stem Cell, 2017, 20(2):177-190

PubMed: 27939219 ( click the link to review the publication )

Cancer Cell, 2017, 31(5):635-652

PubMed: 28434841 ( click the link to review the publication )

Nat Med, 2015, 10.1038/nm.3855

PubMed: 25939062 ( click the link to review the publication )

Cancer Discov, 2015, 5(3):255-63

PubMed: 25564516 ( click the link to review the publication )

Cancer Discov, 2015, 5(3):245-54

PubMed: 25564517 ( click the link to review the publication )

Cancer Cell, 2015, 27(1):97-108

PubMed: 25544637 ( click the link to review the publication )

Cancer Cell, 2015, 27(4):533-46

PubMed: 25873175 ( click the link to review the publication )

Nat Chem Biol, 2021, 10.1038/s41589-021-00751-4

PubMed: 33686292 ( click the link to review the publication )

Nat Commun, 2021, 12(1):1589

PubMed: 33707434 ( click the link to review the publication )

Nat Commun, 2021, 12(1):1920

PubMed: 33772001 ( click the link to review the publication )

Cell Rep, 2021, 34(11):108870

PubMed: 33730585 ( click the link to review the publication )

Cell Rep, 2021, 35(1):108940

PubMed: 33784499 ( click the link to review the publication )

J Clin Endocrinol Metab, 2021, dgab020

PubMed: 33524147 ( click the link to review the publication )

STAR Protoc, 2021, 2(1):100300

PubMed: 33532743 ( click the link to review the publication )

Nat Commun, 2020, 11(1):4684

PubMed: 32943635 ( click the link to review the publication )

Dev Cell, 2020, 55(3):298-313.e10

PubMed: 33171110 ( click the link to review the publication )
Clin Cancer Res, 2020, 10.1158/1078-0432

PubMed: 32034078 ( click the link to review the publication )

Cell Rep, 2020, 31(12):107810

PubMed: 32579936 ( click the link to review the publication )

Eur J Cancer, 2020, 126:93-103

PubMed: 31927215 ( click the link to review the publication )

J Bone Miner Res, 2020, 10.1002/jbmr.4176

PubMed: 32916025 ( click the link to review the publication )

J Exp Clin Cancer Res, 2020, 9;39(1):107

PubMed: 32517736 ( click the link to review the publication )

Br J Cancer, 2020, 22

PubMed: 32439932 ( click the link to review the publication )

Front Immunol, 2020, 11:572960

PubMed: 33013932 ( click the link to review the publication )

Mol Cancer Ther, 2020, 5;molcanther.1131.2019

PubMed: 32371585 ( click the link to review the publication )

Mol Cancer Ther, 2020, 19(3):882-894

PubMed: 31879363 ( click the link to review the publication )

Aging (Albany NY), 2020, 12(19):19022-19044

PubMed: 33044945 ( click the link to review the publication )

J Cancer, 2020, 15;11(12):3492-3501

PubMed: 32284745 ( click the link to review the publication )

J Cell Sci, 2020, 133(14)jcs246306

PubMed: 32513819 ( click the link to review the publication )

Sci Rep, 2020, 4;10(1):4025

PubMed: 32132574 ( click the link to review the publication )

J Biol Chem, 2020, 22;295(21):7418-7430

PubMed: 32312748 ( click the link to review the publication )

Cell Cycle, 2020, 1-15

PubMed: 33064959 ( click the link to review the publication )

Biosci Rep, 2020, 30;40(4):BSR20200905

PubMed: 32285908 ( click the link to review the publication )

Biochem Biophys Res Commun, 2020, 23;S0006-291X(20)30951-7

PubMed: 32456793 ( click the link to review the publication )

G3 (Bethesda), 2020, 4;10(5):1585-1597

PubMed: 32265286 ( click the link to review the publication )

Kaohsiung J Med Sci, 2020, 10.1002/kjm2.12319

PubMed: 33174370 ( click the link to review the publication )

Nat Commun, 2020, 11(1):3627

PubMed: 32686664 ( click the link to review the publication )

BMC Cancer, 2020, 20(1):724

PubMed: 32758183 ( click the link to review the publication )

Nat Med, 2020, 10.1038/s41591-020-0986-1

PubMed: 32719489 ( click the link to review the publication )

J Cell Biol, 2020, 219(2)

PubMed: 31914171 ( click the link to review the publication )

J Invest Dermatol, 2020, S0022-202X(20)31981-3

PubMed: 32805217 ( click the link to review the publication )
Int Immunopharmacol, 2020, 81:106241

PubMed: 32058927 ( click the link to review the publication )

Cell Rep, 2020, 32(3):107933

PubMed: 32697996 ( click the link to review the publication )

Mol Oncol, 2020, 10.1002/1878-0261.12673

PubMed: 32191822 ( click the link to review the publication )

Cancer, 2020, 10.1002/cncr.33071

PubMed: 32767682 ( click the link to review the publication )

Mol Cell, 2019, 10.1016/j.molcel.2019.10.016

PubMed: 31733992 ( click the link to review the publication )

Autophagy, 2019, 10.1080/15548627.2019.1660545

PubMed: 31462148 ( click the link to review the publication )

Autophagy, 2019, 15(3):543-557

PubMed: 30269645 ( click the link to review the publication )

Clin Cancer Res, 2019, 25(21):6429-6442

PubMed: 31439581 ( click the link to review the publication )

Clin Cancer Res, 2019, 25(16):5107-5121

PubMed: 31164374 ( click the link to review the publication )

Curr Biol, 2019, 29(5):737-749

PubMed: 30773367 ( click the link to review the publication )

Cell Rep, 2019, 27(12):3696-3708.e5

PubMed: 31216485 ( click the link to review the publication )

Sci Signal, 2019, 12(612)

PubMed: 31848319 ( click the link to review the publication )

Cancers (Basel), 2019, 11(10)

PubMed: 31627299 ( click the link to review the publication )

J Neurosci, 2019, 39(27):5255-5268

PubMed: 31043483 ( click the link to review the publication )

Cell Death Dis, 2019, 10(8):563

PubMed: 31332166 ( click the link to review the publication )

Cell Chem Biol, 2019, 26(3):366-377

PubMed: 30661989 ( click the link to review the publication )

Curr Biol, 2019, 29(5):737-749

PubMed: 30773367 ( click the link to review the publication )

Am J Cancer Res, 2019, 9(9):1922-1937

PubMed: 31598395 ( click the link to review the publication )

Sci Rep, 2019, 9(1):30

PubMed: 30631106 ( click the link to review the publication )

Sci Rep, 2019, 9(1):9805

PubMed: 31285446 ( click the link to review the publication )

Cancer Gene Ther, 2019, 10.1038/s41417-019-0117-7

PubMed: 31257364 ( click the link to review the publication )

Biochem J, 2019, 476(20):3081-3107

PubMed: 31665227 ( click the link to review the publication )

Front Physiol, 2019, 10:167

PubMed: 30873046 ( click the link to review the publication )

Biomed Res Int, 2019, 2019:5196028

PubMed: 31223619 ( click the link to review the publication )
JCI Insight, 2019, 4(20)124164

PubMed: 31619583 ( click the link to review the publication )

Cell Mol Gastroenterol Hepatol, 2019, 8(1):21-36

PubMed: 30831322 ( click the link to review the publication )

Cancer Biol Med, 2019, 16(1):66-83

PubMed: 31119047 ( click the link to review the publication )

Nat Cell Biol, 2018, 20(4):465-478

PubMed: 29593330 ( click the link to review the publication )

Cancer Res, 2018, 78(12):3176-3189

PubMed: 29661831 ( click the link to review the publication )

Cancer Res, 2018, 78(15):4396-4410

PubMed: 29844118 ( click the link to review the publication )

Cell Death Dis, 2018, 9(11):1063

PubMed: 30337536 ( click the link to review the publication )

J Mol Cell Biol, 2018, 10(4):331-340

PubMed: 29190376 ( click the link to review the publication )

Sci Rep, 2018, 8(1):10784

PubMed: 30018387 ( click the link to review the publication )

Acta Pharmacol Sin, 2018, 39(8):1338-1346

PubMed: 29345254 ( click the link to review the publication )

Front Genet, 2018, 9:690

PubMed: 30671083 ( click the link to review the publication )

Cell Signal, 2018, 44:127-137

PubMed: 29329780 ( click the link to review the publication )

PLoS One, 2018, 13(8):e0200725

PubMed: 30067771 ( click the link to review the publication )

Med Sci Monit, 2018, 24:2735-2743

PubMed: 29720580 ( click the link to review the publication )

Oncol Lett, 2018, 16(4):4561-4566

PubMed: 30214591 ( click the link to review the publication )

J Exp Med, 2018, 215(1):159-175

PubMed: 29141866 ( click the link to review the publication )

Oncol Lett, 2018, 16(1):632-642

PubMed: 29928450 ( click the link to review the publication )

Nat Commun, 2017, 8(1):1090

PubMed: 29061961 ( click the link to review the publication )

Nat Commun, 2017, 8(1):2207

PubMed: 29263324 ( click the link to review the publication )

Nucleic Acids Res, 2017, 45(16):9654-9678

PubMed: 28934469 ( click the link to review the publication )

Cell Syst, 2017,

PubMed: 28017544 ( click the link to review the publication )

Mol Syst Biol, 2017, 13(1):905

PubMed: 28069687 ( click the link to review the publication )

Cell Rep, 2017, 20(10):2341-2356

PubMed: 28877469 ( click the link to review the publication )

Oncogene, 2017, 36(15):2074-2084

PubMed: 27748760 ( click the link to review the publication )
Oncogene, 2017, 36(26):3651-3660

PubMed: 28192408 ( click the link to review the publication )

Sci Signal, 2017, 10(507)eaam7550

PubMed: 29184034 ( click the link to review the publication )

PLoS Pathog, 2017, 13(4):e1006344

PubMed: 28414774 ( click the link to review the publication )

Cell Commun Signal, 2017, 10.1186/s12964-017-0164-3

PubMed: 28143584 ( click the link to review the publication )

Biochim Biophys Acta Mol Cell Res, 2017, 1864(10):1554-1565

PubMed: 28641977 ( click the link to review the publication )

Sci Rep, 2017, 7(1):1535

PubMed: 28484242 ( click the link to review the publication )

Sci Rep, 2017, 7(1):1994

PubMed: 28515443 ( click the link to review the publication )

J Pharm Biomed Anal, 2017, 145:473-481

PubMed: 28743078 ( click the link to review the publication )

PLoS One, 2017, 12(7):e0181243

PubMed: 28704514 ( click the link to review the publication )

Anticancer Res, 2017, 37(3):1083-1090

PubMed: 28314268 ( click the link to review the publication )

Oncotarget, 2017, 8(4):5992-6002

PubMed: 27863413 ( click the link to review the publication )

Oncotarget, 2017, 8(1):979-987

PubMed: 27894090 ( click the link to review the publication )

Oncotarget, 2017, 8(25):41422-41431

PubMed: 28507282 ( click the link to review the publication )

Nat Cell Biol, 2016, 18(5):527-39

PubMed: 27043084 ( click the link to review the publication )

Genome Biol, 2016, 17(1):149

PubMed: 27380875 ( click the link to review the publication )

Nat Chem Biol, 2016, 12(1):22-8

PubMed: 26595461 ( click the link to review the publication )

EMBO J, 2016, 35(17):1902-22

PubMed: 27481935 ( click the link to review the publication )

Mol Oncol, 2016, 10(1):101-12

PubMed: 26471831 ( click the link to review the publication )

Mol Oncol, 2016, 10(4):538-52

PubMed: 26686971 ( click the link to review the publication )

Aging (Albany NY), 2016, 8(2):231-44

PubMed: 26851731 ( click the link to review the publication )

Aging (Albany NY), 2016, 8(12):3535-3551

PubMed: 28077803 ( click the link to review the publication )

J Immunol, 2016, 196(10):4390-9

PubMed: 27067005 ( click the link to review the publication )

Sci Rep, 2016,

PubMed: 27849011 ( click the link to review the publication )

J Biol Chem, 2016, 291(12):6262-71

PubMed: 26755732 ( click the link to review the publication )
Front Plant Sci, 2016, 7:1746

PubMed: 27917191 ( click the link to review the publication )

Cell Signal, 2016, 28(4):284-93

PubMed: 26795954 ( click the link to review the publication )

PLoS One, 2016, 11(4):e0154564

PubMed: 27128484 ( click the link to review the publication )

Biochem Biophys Res Commun, 2016, 478(4):1515-20

PubMed: 27565731 ( click the link to review the publication )

Int J Endocrinol, 2016, 2016:5286972

PubMed: 27990160 ( click the link to review the publication )

Oncotarget, 2016, 7(44):71211-71222

PubMed: 27655717 ( click the link to review the publication )

Oncotarget, 2016, 7(4):3947-65

PubMed: 26678033 ( click the link to review the publication )

Autophagy, 2015, 11(2):285-97

PubMed: 25801301 ( click the link to review the publication )

Cancer Res, 2015, 75(20):4398-406

PubMed: 26359452 ( click the link to review the publication )

Cell Rep, 2015, 11(3):446-59

PubMed: 25865887 ( click the link to review the publication )

J Hematol Oncol, 2015,

PubMed: 26515759 ( click the link to review the publication )

Sci Signal, 2015, 8(375):ra42

PubMed: 25943352 ( click the link to review the publication )

J Med Chem, 2015, 58(16):6559-73

PubMed: 26237138 ( click the link to review the publication )

Cancer Discov, 2015, 5(3):245-54

PubMed: 25564517 ( click the link to review the publication )

Clin Sci, 2015, 128(8):517-26

PubMed: ( click the link to review the publication )

J Cancer, 2015, 6(12):1195-205

PubMed: 26535060 ( click the link to review the publication )

Mol Cancer Res, 2015, 13(2):223-30

PubMed: ( click the link to review the publication )

Mol Cancer Res, 2015, 13(1):120-9

PubMed: ( click the link to review the publication )

Mol Cancer Res, 2015, 13(1):120-9

PubMed: 25232030 ( click the link to review the publication )

Mol Cancer Res, 2015, 13(2):223-30

PubMed: 25582703 ( click the link to review the publication )

Int J Mol Sci, 2015, 16(2):3267-82

PubMed: 25654224 ( click the link to review the publication )

Am J Physiol Endocrinol Metab, 2015, 309(2):E142-53

PubMed: 25968579 ( click the link to review the publication )

J Biol Chem, 2015, 10.1074/jbc.C114.627778

PubMed: 25833948 ( click the link to review the publication )

Stem Cells Int, 2015, 2015:561404

PubMed: 26221145 ( click the link to review the publication )
Biochem J, 2015, 465(2):227-38

PubMed: 25353634 ( click the link to review the publication )

J Chromatogr A, 2015, 1388:267-73

PubMed: 25725958 ( click the link to review the publication )

J Chromatogr A, 2015, 1388:267-73

PubMed: ( click the link to review the publication )

Proteomics, 2015, 10.1002/pmic.201400586

PubMed: 25764225 ( click the link to review the publication )

Cancer Biol Ther, 2015,

PubMed: 25692408 ( click the link to review the publication )

PLoS One, 2015, 10(11):e0143357

PubMed: 26580203 ( click the link to review the publication )

PLoS One, 2015, 10(7):e0131400

PubMed: 26148118 ( click the link to review the publication )

Clin Sci (Lond), 2015, 128(8):517-26

PubMed: 25428582 ( click the link to review the publication )

Oncotarget, 2015, 6(7):5118-33

PubMed: 25742786 ( click the link to review the publication )

Cell Signal, 2015, 27(11):2191-200

PubMed: 26210887 ( click the link to review the publication )

J Clin Invest, 2014, 10.1172/JCI73935

PubMed: 25415435 ( click the link to review the publication )

Nat Commun, 2014, 5:3384

PubMed: 24594970 ( click the link to review the publication )

Autophagy, 2014, 10(6):1120-36

PubMed: 24879157 ( click the link to review the publication )

Leukemia, 2014, 28(5):1092-102

PubMed: 24135829 ( click the link to review the publication )

Cancer Res, 2014, 74(10):2846-56

PubMed: 24614082 ( click the link to review the publication )

Cancer Res, 2014, 74(14):3947-58

PubMed: 24986516 ( click the link to review the publication )

Clin Cancer Res, 2014, 20(22):5756-67

PubMed: 25316808 ( click the link to review the publication )

Int J Cancer, 2014, 10.1002/ijc.28880

PubMed: 24706460 ( click the link to review the publication )

Antioxid Redox Sign, 2014, 20(9):1382-95

PubMed: 24063548 ( click the link to review the publication )

Sci Signal, 2014, 7(349):ra102

PubMed: 25351247 ( click the link to review the publication )

Mol Metab, 2014, 3(6):630-41

PubMed: 25161886 ( click the link to review the publication )

Mol Cell Proteomics, 2014, 13(6):1429-38

PubMed: 24643969 ( click the link to review the publication )

Am J Cancer Res, 2014,

PubMed: 25520871 ( click the link to review the publication )

Am J Cancer Res, 2014, 4(6):824-837

PubMed: ( click the link to review the publication )
Mol Cell Biol, 2014, 34(22):4088-103

PubMed: 25182533 ( click the link to review the publication )

Mol Cell Biol, 2014, 34(13):2517-32

PubMed: 24777602 ( click the link to review the publication )

Mol Reprod Dev, 2014, 81(7):655-65

PubMed: 24753052 ( click the link to review the publication )

Oncol Rep, 2014, 31(2):649-56

PubMed: 24297300 ( click the link to review the publication )

PLoS One, 2014, 9(12):e113555

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Oncotarget, 2014,

PubMed: 25277205 ( click the link to review the publication )

Oncotarget, 2014, 5(21):10732-44

PubMed: 25313141 ( click the link to review the publication )

Oncotarget, 2014, 5(21):10503-17

PubMed: 25361177 ( click the link to review the publication )

Oncotarget, 2014, 5(15):6015-27

PubMed: 24970798 ( click the link to review the publication )

Oncotarget, 2014, 5(18):8737-49

PubMed: 25228590 ( click the link to review the publication )

Blood, 2013, 121(18):3619-30

PubMed: 23444404 ( click the link to review the publication )

EMBO J, 2013, 28;32(17):2377-91

PubMed: 23921550 ( click the link to review the publication )

Cancer Res, 2013, 73(11):3402-11

PubMed: 23585456 ( click the link to review the publication )

Mol Cancer, 2013, 12:81

PubMed: 23886294 ( click the link to review the publication )

Am J Transplant, 2013, 13(8):2035-43

PubMed: 23841834 ( click the link to review the publication )

Sci Signal, 2013, 6(271):ra25

PubMed: 23592840 ( click the link to review the publication )

Mol Cancer Ther, 2013, 13(1):37-48

PubMed: 24233399 ( click the link to review the publication )

Mol Cancer Ther, 2013,

PubMed: 23615631 ( click the link to review the publication )

Mol Oncol, 2013, 33:404-18

PubMed: 24444656 ( click the link to review the publication )

J Hypertens, 2013, 31(2):303-11

PubMed: 23303355 ( click the link to review the publication )

J Biol Chem, 2013, 288(49):35287-96

PubMed: 24133218 ( click the link to review the publication )

Mol Biol Cell, 2013, 24(23):3736-45

PubMed: 24088573 ( click the link to review the publication )

Cancer Chemother Pharmacol, 2013, 71(5):1297-307

PubMed: 23479136 ( click the link to review the publication )

PLoS One, 2013, 8(2):e56567

PubMed: 23451056 ( click the link to review the publication )
Onco Targets Ther, 2013, 6:1373-84

PubMed: 24124380 ( click the link to review the publication )

Biochem Biophys Res Commun, 2013, 440(4):701-6

PubMed: 24103749 ( click the link to review the publication )

Oncol Lett, 2013, 5(2):717-721

PubMed: 23420667 ( click the link to review the publication )

Autophagy, 2012, 9(1):74-92

PubMed: 23196898 ( click the link to review the publication )

Exp Neurol, 2012, 233(2):799-806

PubMed: 22177999 ( click the link to review the publication )

Biochem Pharmacol, 2012, 83(9):1183-94

PubMed: 22305748 ( click the link to review the publication )

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description

Features

First drug to inhibit both types of mTOR protein.

Targets

mTOR (truncated) ^[1] (MDA-MB-468 cells)	mTOR (full length) ^[1] (MDA-MB-468 cells)
0.13 nM	0.8 nM

In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. ^[1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. ^[2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. ^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
LU-139	M4HXSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWPJR\|UxRTBwMEKzN\|Eh\|ryP	MoTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
NCI-H720	NVPPO2lUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFuyeVJKSzVyPUCuNFI6OzhizszN	Mn\XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
KARPAS-45	MlyyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYL0bmJqUUN3ME2wMlA{OzdzIN88US=>	NEDyUYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
EW-3	MmfWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYfxZVl[UUN3ME2wMlA1ODV4IN88US=>	MlvkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
SBC-1	M1fNfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NV[zNm56UUN3ME2wMlA1PThzIN88US=>	NXPZOmtFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
NCI-H209	NUXZSXFuT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MojnTWM2OD1yLkC1OFU{KM7:TR?=	NFHEemI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
EoL-1-cell	NXfid\|lqT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXf2dGN{UUN3ME2wMlA3PTZ7IN88US=>	M1fJSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
H9	M3\FTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NXjqRpdIUUN3ME2wMlA3Pjl{IN88US=>	NHv3T5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
AN3-CA	NVTkdWhvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NIrEU3RKSzVyPUCuNFc6PTFizszN	MnHjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
HH	Ml\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MUTJR\|UxRTBwMEiwNFkh\|ryP	MlvOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
KU812	NYfJWYZCT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MUjJR\|UxRTBwMEiwOFMh\|ryP	NGiyd2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
DU-4475	M1jOd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M{m0dWlEPTB;MD6wPFA1PyEQvF2=	NXu0UFlGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
CTV-1	NYDtSlFET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MmXkTWM2OD1yLkC4OlQyKM7:TR?=	M3HQXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
BV-173	MlTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYC0dG45UUN3ME2wMlA6ODZzIN88US=>	M33mVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
ES5	MlzpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MnnFTWM2OD1yLkC5N\|Uh\|ryP	MmLoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
G-402	Mli5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NFe1[VZKSzVyPUCuNFk6QCEQvF2=	MkjYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
MV-4-11	NXzwb2YzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NEnuc4VKSzVyPUCuNVA{PDdizszN	M1PqdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
NB69	MnjPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M4H2NWlEPTB;MD6xN\|Uh\|ryP	M3XGc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
EGI-1	NWL1Z5ZrT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MWDJR\|UxRTBwMUO1N\|Eh\|ryP	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
EW-18	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVLpUY9OUUN3ME2wMlE1ODd\|IN88US=>	NX;idW5wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
ME-180	MmLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NWOzVGg3UUN3ME2wMlE1PjhzIN88US=>	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
D-283MED	M4L4bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVPLNZpTUUN3ME2wMlE1PzJ\|IN88US=>	M{fHcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
NB14	NWrKdlhpT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NH3pXItKSzVyPUCuNVY1PDdizszN	Mln0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
Ramos-2G6-4C10	M1nDPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MoXZTWM2OD1yLkG2PVg{KM7:TR?=	NGH3U2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
NBsusSR	NFXsWoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVnKdZVvUUN3ME2wMlE4ODF7IN88US=>	NY\LVIVtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
LAMA-84	MlfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MYfJR\|UxRTBwMUiwN\|Ih\|ryP	MojiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
NCI-H1581	NX3pUHM2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NUD0OYFiUUN3ME2wMlE5QTZ4IN88US=>	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
A204	MonIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MWDJR\|UxRTBwMUi5O{DPxE1?	NGnQS249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
A549	NU\lZmdNT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MWnJR\|UxRTBwMUm1OUDPxE1?	NV71eVVtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
CAL-51	MorlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MknQTWM2OD1yLkG5OlUyKM7:TR?=	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NKM-1	MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkfLTWM2OD1yLkKwNFI6KM7:TR?=	M2f4XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
LoVo	M1\i[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MoDxTWM2OD1yLkKwNlAzKM7:TR?=	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
HAL-01	NHnk[ZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MlLITWM2OD1yLkKwNlk4KM7:TR?=	NGnldJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
HCC2218	MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Mkn5TWM2OD1yLkKwOlg5KM7:TR?=	NWXDcI5TRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
BEN	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NY[wXYRjUUN3ME2wMlIyOjl\|IN88US=>	MlLwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
LC-2-ad	NHLkd\|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MlXMTWM2OD1yLkKyNFg1KM7:TR?=	NWPqPJRCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
LXF-289	NIPiVXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MULJR\|UxRTBwMkWwOFEh\|ryP	NXXBfWxMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
HGC-27	NULhW4I1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NEGyTWpKSzVyPUCuNlY1OTRizszN	NGX0VGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
LK-2	Mk[2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MofITWM2OD1yLkK2PFI1KM7:TR?=	NWnVRXRZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
CTB-1	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkDJTWM2OD1yLkK3NlE2KM7:TR?=	MkLwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
697	M3ixZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NHrPOpBKSzVyPUCuNlc{PjdizszN	MmDkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
ES7	NEHNVYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NFHnZm5KSzVyPUCuNlc{QDNizszN	MlTXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
MG-63	M4DONmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NEXJVohKSzVyPUCuNlc1ODdizszN	M4j0Z\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
T47D	M{C0PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXLJR\|UxRTBwMke0O\|Uh\|ryP	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
RPMI-8226	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NGTXO3pKSzVyPUCuNlg2QDlizszN	M2nkOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
NCI-H292	M3rKNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWfJR\|UxRTBwMkmxOlQh\|ryP	NGXZSZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
HT	Mn;3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYXmfIhNUUN3ME2wMlI6OzhizszN	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
A3-KAW	MlSwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MnnMTWM2OD1yLkK5O\|Qh\|ryP	Mn;NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
LB2241-RCC	NULEVFZHT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXm3[otjUUN3ME2wMlMxOTl6IN88US=>	NW\QUZU5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
BB65-RCC	MoDES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2SzbWlEPTB;MD6zNFgxPSEQvF2=	M3HQflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
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RH30	MV3BdI9xfG:\|aYOgRZN{[Xl?	MkPwNUDPxE1?	MYC0PEBp		MVXpcoNz\WG\|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDBRnQuPzN5	MlXYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{OyNVgoRjJ2MUOzNlE5RC:jPh?=
Hep-2	NYPp[YxwT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NXHpNoJmOC5yMT2xJO69VQ>?	MUKxNk06PiCq		MUPpcoNz\WG\|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI>	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyM{e0PUc,OjRzMEO3OFk9N2F-
SCC-9	MmmzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NFrTcWoxNjBzLUGg{txO	NV;pSZJFOTJvOU[gbC=>		M{m5O4lv[3KnYYPld{Bk\WyuIHTlZZRpKGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>?	MlvnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMEO3OFkoRjJ2MUCzO\|Q6RC:jPh?=
Hep-2	NULqZpZKTnWwY4Tpc44hSXO\|YYm=	NVvudHN7OC5zL{CuOUDPxE1?	MmTTNlQhcA>?		M2W1[4Jtd2OtczDtWG9TSzFiYX7kJI1VV1KFMjDhZ5RqfmG2aX;u	M4jmNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUCzO\|Q6Lz5{NEGwN\|c1QTxxYU6=
SCC-9	NX;pcYtiTnWwY4Tpc44hSXO\|YYm=	NFnaS5MxNjFxMD61JO69VQ>?	MkHTNlQhcA>?		M2HEe4Jtd2OtczDtWG9TSzFiYX7kJI1VV1KFMjDhZ5RqfmG2aX;u	Mn3YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMEO3OFkoRjJ2MUCzO\|Q6RC:jPh?=
PANC-1	MVjBdI9xfG:\|aYOgRZN{[Xl?	NV;hemdqPTByIH7N	NWXMV\|M3PDhiaB?=		NH;xOnJ{\W6\|aYTpfoV{KHCjbnPy[YF1cWNiY3HuZ4VzKGOnbHzzJJRwKHKjZHnveIhmemGyeR?=	M4rpclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OEi2Nlk1Lz5{M{i4OlI6PDxxYU6=
RMG2	Ml30RZBweHSxc3nzJGF{e2G7	M{O0[lEuOTByMDDuUS=>	NIKxb2s4OiCq	M4r5bGZDWw>?	NFrYbnZqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJkh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=>	NF;Qb4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xOVY{OSd-MkO2NVU3OzF:L3G+
HAC2	M4fWc2Fxd3C2b4Ppd{BCe3OjeR?=	MmLxNU0yODByIH7N	M4LEbVczKGh?	M2C3eGZDWw>?	NUHsSoJpcW6qaXLpeJMhfGinIHPlcIwhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk>	NGLpeIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xOVY{OSd-MkO2NVU3OzF:L3G+
HT-29	NE\BUYdCeG:ydH;zbZMhSXO\|YYm=	NISzd5AyKM7:TR?=	NGHjOYY4OiCq		MXrheJRmdnWjdHXzJFUuTlVvaX7keYNm\CClZXzsJIRm[XSq	MmnYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEmxNlEoRjJzOUS5NVIyRC:jPh?=
DLD1	M4HIOGFxd3C2b4Ppd{BCe3OjeR?=	NIHQdZkyKM7:TR?=	MV[3NkBp		NFfjUGxifHSnboXheIV{KDVvRmWtbY5lfWOnZDDj[YxtKGSnYYTo	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OUGyNUc,OjF7NEmxNlE9N2F-
HT-29	M33USWZ2dmO2aX;uJGF{e2G7	M1jwdVEh\|ryP	NGr0NVY4OiCq		NEjucplqdmS3Y3XzJIF2fG:yaHHnfUB1dyClb37m[ZIh[3m2b4Dyc5Rm[3Srb36=	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OUGyNUc,OjF7NEmxNlE9N2F-
DLD1	NID2dlNHfW6ldHnvckBCe3OjeR?=	MkTNNUDPxE1?	NILB[ZE4OiCq		M321folv\HWlZYOgZZV1d3CqYXf5JJRwKGOxbn\ldkBkgXSxcILveIVkfGmxbh?=	MmTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEmxNlEoRjJzOUS5NVIyRC:jPh?=
Rh41	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MWTJR\|UxRTNyLkegcm0>	MkHNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|M{e2O\|koRjJzM{O3Olc6RC:jPh?=
Rh18	MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MVPJR\|UxRTRzLkSgcm0>	M2XvcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO\|Y4QTxxYU6=
Rh30	M1;4Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGLvN\|RKSzVyPUG0JI5O	NYTsbXljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
BT-12	M2D2XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NWjkSmJYUUN3ME2yOE44KG6P	M1PqUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO\|Y4QTxxYU6=
CHLA-266	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4[yWmlEPTB;MT6xJI5O	NFvvRm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
TC-71	Ml\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NV;BW5BRUUN3ME20Ok42KG6P	NHn2W5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
CHLA-9	M3XrPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M2HESGlEPTB;MUWuN{BvVQ>?	NEflOok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
CHLA-10	NX31Z4d[T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVfKfVN[UUN3ME2zMlkhdk1?	Mk\uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|M{e2O\|koRjJzM{O3Olc6RC:jPh?=
CHLA-258	NFjFXHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmXCTWM2OD1zMD60JI5O	NGLVb\|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
GBM2	Mmf0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MlzNTWM2OD12MD63JI5O	NFHCUHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
NB-1643	MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NV\3VmNbUUN3ME2zN{45KG6P	M3zIcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO\|Y4QTxxYU6=
NB-EBc1	M4G2O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVjtTXNiUUN3ME2yO{4yKG6P	NHHPcJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
CHLA-90	NFS2Tm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				Ml\6TWM2OD1\|ND61JI5O	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN\|N{[3PUc,OjF\|M{e2O\|k9N2F-
CHLA-136	MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M1LjR2lEPTB;MU[yMlYhdk1?	M3;uWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO\|Y4QTxxYU6=
NALM-6	NYTPR4l4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnHTTWM2OD1\|Mj65JI5O	NYnKRVVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
COG-LL-317	NEjZcXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUHHO4RKUUN3ME2xMlMhdk1?	MkHpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|M{e2O\|koRjJzM{O3Olc6RC:jPh?=
RS4;11	M1nYWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1LZc2lEPTB;M{Wgcm0>	M3:wcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO\|Y4QTxxYU6=
MOLT-4	NFjO[YdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MXnJR\|UxRThwNjDuUS=>	MlGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|M{e2O\|koRjJzM{O3Olc6RC:jPh?=
CCRF-CEM	NXLjU2FOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MoHFTWM2OD12LkGgcm0>	NWLjXJYyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
Kasumi-1	M1[zb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NEXWOnRKSzVyPUGuOEBvVQ>?	MkPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|M{e2O\|koRjJzM{O3Olc6RC:jPh?=
Karpas-299	MnHGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MVTJR\|UxRTJzLkegcm0>	NFK5d\|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
Ramos-RA1	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmPWTWM2OD16LkKgcm0>	MmT1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|M{e2O\|koRjJzM{O3Olc6RC:jPh?=
H838	NWrKUpRJTnWwY4Tpc44hSXO\|YYm=	NGHZRpIyKM7:TR?=	M2TyWVQ5KGh?		MVvpcoR2[2W\|IHPlcIwh[XW2b4DoZYd6	NHTDRXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEO2OFEyOyd-MkCzOlQyOTN:L3G+
H838	NEjRelFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NFj5NoQxNTNizszN	NXfJdW1oPzJiaB?=		NXm1d\|VMcW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7	NE\nN5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MECyPFg2PCd-MkCwNlg5PTR:L3G+
A549	NY\NZW9MT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Mo\6NE0{KM7:TR?=	MlTFO\|IhcA>?		M1zTNYlv\HWlZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKGG3dH;wbIFogQ>?	NHXqU2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MECyPFg2PCd-MkCwNlg5PTR:L3G+

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; PubMed: 24457069 Breast Cancer Res Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙 p-EGFR / EGFR; PubMed: 25654224 Int J Mol Sci PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot; p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; PubMed: 25654224 Int J Mol Sci PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.	24457069 25654224
Immunofluorescence	p-4EBP1 / EIF4E / mTOR; PubMed: 24600487 Int J Clin Exp Med (C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋𢡄�෋䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemｾ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒ｾ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wｾ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v 玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗 LC3B / p62 ; PubMed: 30337536 Cell Death Dis Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞	24600487 30337536
Growth inhibition assay	Cell viability ; PubMed: 29263324 Nat Commun Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.	29263324

In vivo

AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. ^[1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. ^[4]

Protocol

Kinase Assay:^[1]	- Collapse Cell-based determinations of mTOR inhibition: A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:^[1]	- Collapse Cell lines: U87MG, A549 and H838 cells Concentrations: 1 nM - 1 μM Incubation Time: 72 to 96 hours Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting. (Only for Reference)
Animal Research:^[1]	- Collapse Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk). Dosages: 2.5-20 mg/kg Administration: Oral gavage once or twice daily (Only for Reference)

References

[4] García-Martínez JM, et al. Br J Cancer, 2011, 104(7), 1116-1

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Solubility (25°C)

In vitro	DMSO	50 mg/mL warmed (107.4 mM)
	Ethanol	3 mg/mL (6.44 mM)
	Water	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 4% DMSO+30% PEG 300+ddH2O For best results, use promptly after mixing.	5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download AZD8055 SDF

Molecular Weight	465.54
Formula	C₂₅H₃₁N₅O₄
CAS No.	1009298-09-2
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CC1COCCN1C2=NC(=NC3=C2C=CC(=N3)C4=CC(=C(C=C4)OC)CO)N5CCOCC5C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

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Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

Selective mTOR inhibitor

Rapamycin (Sirolimus) : mTORC1-selective, IC50=~0.1 nM.
Most Potent mTOR Inhibitor

Rapamycin (Sirolimus) : mTOR, IC50=~0.1 nM.
FDA-approved mTOR Inhibitor

Temsirolimus (CCI-779, NSC 683864) : Approved by FDA for Renal Cell Carcinoma (RCC).
Newest mTOR Inhibitor

XL388 ^New : Highly potent, selective, ATP-competitive inhibitor of mTOR with IC50 of 9.9 nM, 1000-fold selectivity over the closely related PI3K kinases.

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CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.
Dorsomorphin (Compound C) ^New

Dorsomorphin (Compound C, BML-275) is a potent, reversible, selective AMPK inhibitor with K_i of 109 nM in cell-free assays, exhibiting no significant inhibition of several structurally related kinases including ZAPK, SYK, PKCθ, PKA, and JAK3. Dorsomorphin selectively inhibits the BMP type I receptors ALK2, ALK3 and ALK6. Dorsomorphin is used in promoting specific cell differentiation and inducing cancer cell line autophagy. For animal testing, the water-soluble S7306 Dorsomorphin (Compound C) 2HCl is recommended.
Rapamycin (Sirolimus)

Rapamycin (Sirolimus, AY 22989, NSC-2260804) is a specific mTOR inhibitor with IC50 of ~0.1 nM HEK293 cells.
Everolimus (RAD001)

Everolimus (RAD001, SDZ-RAD) is an mTOR inhibitor of FKBP12 with IC50 of 1.6-2.4 nM in a cell-free assay. Everolimus induces cell apoptosis and autophagy and inhibits tumor cells proliferation.
Torin 1

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Temsirolimus (CCI-779)

Temsirolimus (CCI-779, NSC 683864) is a specific mTOR inhibitor with IC50 of 1.76 μM in a cell-free assay. Temsirolimus induces autophagy and apoptosis.
Sapanisertib (MLN0128)

Sapanisertib (MLN0128, INK 128, TAK-228) is a potent and selective mTOR inhibitor with IC50 of 1 nM in cell-free assays; >200-fold less potent to class I PI3K isoforms, superior in blocking mTORC1/2 and sensitive to pro-invasion genes (vs Rapamycin). Phase 1.
Tacrolimus (FK506)

Tacrolimus (FK506, FR900506, Fujimycin, Prograf) is a 23-membered macrolide lactone, it reduces peptidyl-prolyl isomerase activity in T cells by binding to the immunophilin FKBP12 (FK506 binding protein) creating a new complex. Tacrolimus also inhibits the phosphatase activity of calcineurin. Tacrolimus induces vascular endothelial autophagy.
KU-0063794

KU-0063794 is a potent and highly specific dual-mTOR inhibitor of mTORC1 and mTORC2 with IC50 of ~10 nM in cell-free assays; no effect on PI3Ks.

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AZD8055