AZD8055

For research use only.

Catalog No.S1555

138 publications

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.

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Selleck's AZD8055 has been cited by 138 publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. AZD8055 induces caspase-dependent apoptosis and also induces autophagy. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTBwMEKzN|Eh|ryP NGryUWVUSU6JRWK=
NCI-H720 NVzUeGRlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTBwMEK5N|gh|ryP M4fBNXNCVkeHUh?=
KARPAS-45 M1\lbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;nUVZKSzVyPUCuNFM{PzFizszN NWPP[JdzW0GQR1XS
EW-3 NV;vOZJzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTBwMESwOVYh|ryP MXHTRW5ITVJ?
SBC-1 NXLFPFJlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;lcpBKSzVyPUCuNFQ2QDFizszN MX3TRW5ITVJ?
NCI-H209 NXnrRWFHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\yPFZNUUN3ME2wMlA2PDV|IN88US=> NWjHUHA2W0GQR1XS
EoL-1-cell MmT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTBwME[1Olkh|ryP M{nTU3NCVkeHUh?=
H9 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\wRWFJUUN3ME2wMlA3Pjl{IN88US=> NUKzeWlxW0GQR1XS
AN3-CA NUfFXYhtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXEUlJJUUN3ME2wMlA4QTVzIN88US=> MlviV2FPT0WU
HH NWT4ZYdGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vBPGlEPTB;MD6wPFAxQSEQvF2= NIfJZYtUSU6JRWK=
KU812 M2LBeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvsUlZyUUN3ME2wMlA5ODR|IN88US=> M13mU3NCVkeHUh?=
DU-4475 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzOW3NKSzVyPUCuNFgxPDdizszN MknNV2FPT0WU
CTV-1 NFPxZlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3VTWM2OD1yLkC4OlQyKM7:TR?= M3\2SHNCVkeHUh?=
BV-173 NGDFNXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHFV2pKSzVyPUCuNFkxPjFizszN MWjTRW5ITVJ?
ES5 M17EbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETT[mVKSzVyPUCuNFk{PSEQvF2= NXf5[|E6W0GQR1XS
G-402 NInCVmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHT3TWRKSzVyPUCuNFk6QCEQvF2= NWfGN2xVW0GQR1XS
MV-4-11 NHrCXppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoSyTWM2OD1yLkGwN|Q4KM7:TR?= NH7yVXJUSU6JRWK=
NB69 NWXUSZV6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3vbYp7UUN3ME2wMlE{PSEQvF2= NH\ycmpUSU6JRWK=
EGI-1 M3rJSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIS2[mRKSzVyPUCuNVM2OzFizszN MVXTRW5ITVJ?
EW-18 NUW3WI1VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4noO2lEPTB;MD6xOFA4OyEQvF2= NWm3PHZNW0GQR1XS
ME-180 NHL5flVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LVS2lEPTB;MD6xOFY5OSEQvF2= NYSxUm83W0GQR1XS
D-283MED NGDhRVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTBwMUS3NlMh|ryP NXn5Z4V5W0GQR1XS
NB14 M2jwTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnLdJNKSzVyPUCuNVY1PDdizszN NVyyZWxiW0GQR1XS
Ramos-2G6-4C10 M{XBdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTBwMU[5PFMh|ryP MVPTRW5ITVJ?
NBsusSR NGm4O3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TFTmlEPTB;MD6xO|AyQSEQvF2= NVrOV|FXW0GQR1XS
LAMA-84 MmHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7R[Ws1UUN3ME2wMlE5ODN{IN88US=> NF62R29USU6JRWK=
NCI-H1581 NYO0SGpuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonsTWM2OD1yLkG4PVY3KM7:TR?= NYf4fnRWW0GQR1XS
A204 NIrySWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIT6d2NKSzVyPUCuNVg6PyEQvF2= NVzYd21RW0GQR1XS
A549 NHy4eoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTBwMUm1OUDPxE1? Ml\DV2FPT0WU
CAL-51 NIDNfmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\tTWM2OD1yLkG5OlUyKM7:TR?= MUPTRW5ITVJ?
NKM-1 NHLE[VNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTBwMkCwNlkh|ryP MoLwV2FPT0WU
LoVo NX7hbJA3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;pTWM2OD1yLkKwNlAzKM7:TR?= NEHaRllUSU6JRWK=
HAL-01 M2LOZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rIS2lEPTB;MD6yNFI6PyEQvF2= M{\HZ3NCVkeHUh?=
HCC2218 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jGS2lEPTB;MD6yNFY5QCEQvF2= Mom5V2FPT0WU
BEN NUfiV5JNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInC[4RKSzVyPUCuNlEzQTNizszN NWjEeHlUW0GQR1XS
LC-2-ad MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPzTWM2OD1yLkKyNFg1KM7:TR?= NXrFfZl2W0GQR1XS
LXF-289 NXH6R3RQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjuTWM2OD1yLkK1NFQyKM7:TR?= MVrTRW5ITVJ?
HGC-27 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTBwMk[0NVQh|ryP NFWwdIVUSU6JRWK=
LK-2 NWfVU|ZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXOR5J5UUN3ME2wMlI3QDJ2IN88US=> MkHCV2FPT0WU
CTB-1 MoXZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TzbGlEPTB;MD6yO|IyPSEQvF2= MlToV2FPT0WU
697 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYj5[ZpRUUN3ME2wMlI4OzZ5IN88US=> MXvTRW5ITVJ?
ES7 M1HkXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rZbmlEPTB;MD6yO|M5OyEQvF2= M4rXSnNCVkeHUh?=
MG-63 M3PHW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfldZF4UUN3ME2wMlI4PDB5IN88US=> NHPnWFVUSU6JRWK=
T47D NVrXd3lCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jnXGlEPTB;MD6yO|Q4PSEQvF2= MnLhV2FPT0WU
RPMI-8226 NFjINpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrzOpNKSzVyPUCuNlg2QDlizszN M3K5U3NCVkeHUh?=
NCI-H292 M{nYVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGOyVZlKSzVyPUCuNlkyPjRizszN MV7TRW5ITVJ?
HT MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4qwV2lEPTB;MD6yPVM5KM7:TR?= NYnRb3BnW0GQR1XS
A3-KAW Ml;qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGToT2tKSzVyPUCuNlk4PCEQvF2= MorSV2FPT0WU
LB2241-RCC NH7OSIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjPZlhKSzVyPUCuN|AyQThizszN NHr6SVdUSU6JRWK=
BB65-RCC MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLtV4k{UUN3ME2wMlMxQDB3IN88US=> M{\pVHNCVkeHUh?=
G-401 NEjmUFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDa[mF3UUN3ME2wMlMxQTh4IN88US=> M4\JenNCVkeHUh?=
TYK-nu MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTBwM{G4OlQh|ryP M1P1SnNCVkeHUh?=
NCI-SNU-1 MkmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzKTWM2OD1yLkOyN|MyKM7:TR?= M1r0ZXNCVkeHUh?=
CHL-1 NV7RUopNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnZWVNVUUN3ME2wMlMzPzB|IN88US=> MlrmV2FPT0WU
P30-OHK MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2L4[mlEPTB;MD6zNlcxPSEQvF2= MXzTRW5ITVJ?
PA-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn[2TWM2OD1yLkOzOlE6KM7:TR?= M1rMTXNCVkeHUh?=
LB1047-RCC M17Ed2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlWzTWM2OD1yLkO0NFQ4KM7:TR?= MYTTRW5ITVJ?
EM-2 NYW5dmJ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDVUXRKSzVyPUCuN|QyQDhizszN M2q0ZXNCVkeHUh?=
VA-ES-BJ NFX3PXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXI[3Q4UUN3ME2wMlM1OjZ6IN88US=> NFO3dllUSU6JRWK=
NCI-H520 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LLdGlEPTB;MD6zOFg{PCEQvF2= NYe2XJV1W0GQR1XS
ECC10 MnWwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DsdWlEPTB;MD6zOVA{PSEQvF2= M3K1U3NCVkeHUh?=
LNCaP-Clone-FGC NVexdYlCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rLOmlEPTB;MD6zOVMyPCEQvF2= NV[0OYdmW0GQR1XS
ES8 NWfUSZV3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXr4eZd1UUN3ME2wMlM3PTV|IN88US=> MmfrV2FPT0WU
HuO-3N1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrKTWM2OD1yLkO4N|c6KM7:TR?= MVfTRW5ITVJ?
L-363 NEHYXoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjTU3pKUUN3ME2wMlM6ODJ4IN88US=> MkjhV2FPT0WU
SK-LU-1 NE\CPVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVOySZdKUUN3ME2wMlM6OjB6IN88US=> NGTFOYVUSU6JRWK=
MCF7 NIHM[nZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTDTWM2OD1yLkSwOVMh|ryP NYm3dY1QW0GQR1XS
HuO9 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jvdWlEPTB;MD60NFY3PyEQvF2= NWDmZlBbW0GQR1XS
BHT-101 NFmxR|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXpcZlSUUN3ME2wMlQxPzh5IN88US=> MmXlV2FPT0WU
MZ2-MEL MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\HXW5PUUN3ME2wMlQxQDh{IN88US=> MWLTRW5ITVJ?
NOS-1 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF:zOpRKSzVyPUCuOFExPzRizszN NGDVWopUSU6JRWK=
NEC8 MlPqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX73VJB3UUN3ME2wMlQyPjl3IN88US=> M1rye3NCVkeHUh?=
COR-L88 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTBwNEKyOlch|ryP M1nJfHNCVkeHUh?=
MFM-223 NVm0NnBpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTBwNEK2NVch|ryP NVqxXHFjW0GQR1XS
HSC-3 NH\HNI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTBwNEK5NFgh|ryP NWnTbm9WW0GQR1XS
OVCAR-5 NHXSZ5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHOTWM2OD1yLkSyPVM{KM7:TR?= NIX5coNUSU6JRWK=
MFE-280 Mn75S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkW5TWM2OD1yLkSzNFMzKM7:TR?= NYXLVY03W0GQR1XS
KP-N-YS M3:5b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfRTWM2OD1yLkSzNFch|ryP MYjTRW5ITVJ?
EPLC-272H MnOwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7DTWM2OD1yLkSzNFc4KM7:TR?= NVnsS3RtW0GQR1XS
KE-37 MoTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfKRppKSzVyPUCuOFMzPDdizszN MofYV2FPT0WU
HuH-7 MmPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTBwNEOzO|Ih|ryP MW\TRW5ITVJ?
CHP-212 NG\tXnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrOUGRKSzVyPUCuOFM3QTdizszN NYj5d5FkW0GQR1XS
SK-MES-1 M4rISWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDtTWM2OD1yLkSzPFQ5KM7:TR?= NYLQXYk5W0GQR1XS
SJSA-1 NVXLfIxWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTBwNES1OVQh|ryP NXi5RYdDW0GQR1XS
QIMR-WIL MorqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XHVGlEPTB;MD60OVE2KM7:TR?= MVvTRW5ITVJ?
ES4 M{LMOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV24W2w6UUN3ME2wMlQ2OzN4IN88US=> NGTpSJJUSU6JRWK=
TI-73 Ml7IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH64PVlKSzVyPUCuOFU3QDZizszN M2fVU3NCVkeHUh?=
COLO-684 MmDZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\xVZdKSzVyPUCuOFU4OjZizszN NH7Wb5hUSU6JRWK=
GCIY MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTBwNEW3OVQh|ryP NVPPXIJ3W0GQR1XS
JVM-3 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{npSGlEPTB;MD60OlI6OiEQvF2= MUnTRW5ITVJ?
MPP-89 MmrRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFWzfIFKSzVyPUCuOFY6PDdizszN NXTOeY52W0GQR1XS
M14 M3PI[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTBwNEexO{DPxE1? NHS4bXNUSU6JRWK=
SW962 NEn6O29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnBWolKSzVyPUCuOFcyQSEQvF2= NH3ad4xUSU6JRWK=
VM-CUB-1 NUfrToFKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\TU3BKSzVyPUCuOFczQDZizszN MnzKV2FPT0WU
A101D NULPfph3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVq3W4g6UUN3ME2wMlQ5QTZ6IN88US=> MWXTRW5ITVJ?
DB MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4WydWlEPTB;MD61NFAzOSEQvF2= Mn;2V2FPT0WU
KNS-62 NVjSbpRvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PJS2lEPTB;MD61NFU3QCEQvF2= NGnaTnRUSU6JRWK=
NCI-H460 NEfWcJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwNUC1PVch|ryP MYDTRW5ITVJ?
MDA-MB-361 Ml7rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTBwNUC4O{DPxE1? MnLUV2FPT0WU
DOK MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\DTWM2OD1yLkWxN|M{KM7:TR?= MoXzV2FPT0WU
CAL-39 NHvOVW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTBwNUKyOlgh|ryP MX3TRW5ITVJ?
HT-1080 NYTLRmlxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fpZWlEPTB;MD61NlU1OyEQvF2= MWrTRW5ITVJ?
GR-ST M13OUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XrdWlEPTB;MD61N|AyPSEQvF2= M1zSTHNCVkeHUh?=
J-RT3-T3-5 NGLIT5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXWwV2p1UUN3ME2wMlU{OTl3IN88US=> NXzaO2E3W0GQR1XS
NCI-H1793 M1PFWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1ntVmlEPTB;MD61N|Q1PyEQvF2= MonJV2FPT0WU
NCI-H1355 NFuyNZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TYRWlEPTB;MD61N|g4QCEQvF2= M4jvTXNCVkeHUh?=
YH-13 NGHlRVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3z6cmlEPTB;MD61OFk{QCEQvF2= MWjTRW5ITVJ?
LOXIMVI MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfC[3VUUUN3ME2wMlU2OTJ|IN88US=> MmXXV2FPT0WU
ES1 M{PsV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnofnJKSzVyPUCuOVU2OThizszN MmjDV2FPT0WU
GAMG MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTBwNUW2OVgh|ryP MnjaV2FPT0WU
SK-MEL-28 M{Sycmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTBwNUW5NUDPxE1? NFvlPYdUSU6JRWK=
KG-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIK4PY9KSzVyPUCuOVY5QDRizszN MoXTV2FPT0WU
HOS M2C1ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnTdFNKSzVyPUCuOVcyQTJizszN M{nZXHNCVkeHUh?=
PANC-08-13 NUjtbmdMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTBwNUezPFkh|ryP M3rUcXNCVkeHUh?=
SW780 MnHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwNUe1PVUh|ryP NWHxSXBlW0GQR1XS
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HEL MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTFwOUSxPFQh|ryP M{nZXXNCVkeHUh?=
SW684 NWLGTlJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PZcGlEPTB;MT65O|M4OSEQvF2= MVnTRW5ITVJ?
NCI-H1693 M3Hq[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYC4[Yh3UUN3ME2xMlk5ODh4IN88US=> MmDSV2FPT0WU
8-MG-BA MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DVTmlEPTB;MT65PFIyKM7:TR?= MULTRW5ITVJ?
OVCAR-8 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTETWM2OD1{LkCwNlk2KM7:TR?= NIPqOYlUSU6JRWK=
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KYSE-520 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTJwMEKwOFkh|ryP NHPaPXpUSU6JRWK=
GB-1 MnvyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoG3TWM2OD1{LkCyOVc4KM7:TR?= NF;FfJVUSU6JRWK=
RCC10RGB MoLnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGj4PGhKSzVyPUKuNFQ5PzFizszN MmfrV2FPT0WU
SW954 NXzQTIdiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\jTWM2OD1{LkC1NFM3KM7:TR?= NX7p[5J{W0GQR1XS
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AU565 MnPuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1PURWlEPTB;Mj6wOVkxPSEQvF2= MonxV2FPT0WU
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NCI-H1395 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTJwMUC5PFYh|ryP MmLsV2FPT0WU
CAS-1 M3vMbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrSbVBKSzVyPUKuNVE2PDZizszN MlLiV2FPT0WU
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HCE-4 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2fFV2lEPTB;Mj60PVQxOiEQvF2= NILuV2NUSU6JRWK=
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H-EMC-SS NHfiXWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTJwNUS2OUDPxE1? M{jne3NCVkeHUh?=
SK-HEP-1 NYDhNHN4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYK0WGxMUUN3ME2yMlU2OjN4IN88US=> M1\OWHNCVkeHUh?=
HCC1395 M376c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1q1UGlEPTB;Mj61OVY2PSEQvF2= NIqzU4tUSU6JRWK=
HuCCT1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7GTWM2OD1{LkW2N|kh|ryP MXLTRW5ITVJ?
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NCI-H1666 MnfhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTJwNUmwO|Yh|ryP NHvN[JFUSU6JRWK=
UMC-11 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUW2TWxVUUN3ME2yMlU6QDZ6IN88US=> NVHiZlBzW0GQR1XS
COLO-679 NELadlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XpUWlEPTB;Mj62NVI{OyEQvF2= M4i0TnNCVkeHUh?=
A388 NXThRpRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlqzTWM2OD1{Lk[1NlU{KM7:TR?= M4nMS3NCVkeHUh?=
LB2518-MEL MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfIR|A1UUN3ME2yMlY2PDB6IN88US=> NWXhNJJHW0GQR1XS
EW-13 NHy3TldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoToTWM2OD1{Lk[4O|Y5KM7:TR?= MUDTRW5ITVJ?
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HOP-92 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTJwN{CxNUDPxE1? MkjjV2FPT0WU
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NCI-H810 NG\S[W5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXsTWM2OD1{LkeyNVk5KM7:TR?= M162UHNCVkeHUh?=
769-P NVrsN3ZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XncWlEPTB;Mj63NlI5KM7:TR?= MXTTRW5ITVJ?
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M059J MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\XTWM2OD1{Lke4N|E5KM7:TR?= M2K3b3NCVkeHUh?=
RD MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFm3UFdKSzVyPUKuO|k1PzFizszN NYjhbGlRW0GQR1XS
GT3TKB NH;mdXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfUTWM2OD1{LkizOFM{KM7:TR?= MX;TRW5ITVJ?
MZ7-mel Mn;CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF[5WmpKSzVyPUKuPFY2ODJizszN MXnTRW5ITVJ?
KYSE-510 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTJwOEm1NFQh|ryP NInudZpUSU6JRWK=
NCI-N87 MnjaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGjDcmZKSzVyPUKuPVE5ODdizszN MV\TRW5ITVJ?
MKN1 Mk\GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3P4eGlEPTB;Mz6wNFE3OyEQvF2= MnHxV2FPT0WU
NCI-H2170 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUXqVW9kUUN3ME2zMlAxPDVzIN88US=> MVrTRW5ITVJ?
OVCAR-3 M{LmWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTNwMEKxNVUh|ryP M3nN[XNCVkeHUh?=
KLE MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rTUmlEPTB;Mz6wOlgyOiEQvF2= M{DKe3NCVkeHUh?=
LB771-HNC NHHyOoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIP1c3hKSzVyPUOuNFk{PDNizszN MmPFV2FPT0WU
MC116 M{TjO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmC0TWM2OD1|LkGxNVEzKM7:TR?= NFjwflVUSU6JRWK=
SCC-9 NXnDc2NoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjzRnBlUUN3ME2zMlEyOzZ4IN88US=> NFrSVWRUSU6JRWK=
U031 MlXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHK3VmhKSzVyPUOuNVI1OjNizszN MYTTRW5ITVJ?
LCLC-103H MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nj[2lEPTB;Mz6yN|g6PCEQvF2= NYDvXlRMW0GQR1XS
U-266 NFKyTmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLa[I1lUUN3ME2zMlI4PjN|IN88US=> MmrkV2FPT0WU
JVM-2 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTNwM{KxOlQh|ryP MWrTRW5ITVJ?
UACC-257 NG[zRotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvrR5dFUUN3ME2zMlQ3PDNzIN88US=> MlTLV2FPT0WU
LB996-RCC NXzO[481T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DUXmlEPTB;Mz60OlUxOiEQvF2= NX;sc|dFW0GQR1XS
BT-549 NFzZXZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTNwNUCyNFkh|ryP NVvy[HZ4W0GQR1XS
NCI-H526 NYXMXY9NT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFGzOVNKSzVyPUOuOVQyPThizszN M4fLU3NCVkeHUh?=
RH-18 MoXOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17IUmlEPTB;Mz62NVc6QCEQvF2= M1fhUXNCVkeHUh?=
SW626 NGLifnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHP5d3FKSzVyPUOuO|EyOjhizszN M2j1NnNCVkeHUh?=
SK-N-AS MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnG5TWM2OD1|LkezNlQ4KM7:TR?= M{CwdXNCVkeHUh?=
IGR-1 MnjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HOT2lEPTB;Mz63OFI1QCEQvF2= NX7xRoVOW0GQR1XS
NCI-H2291 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTNwN{WzN|Yh|ryP NGTjbolUSU6JRWK=
NCI-H441 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37zO2lEPTB;Mz64NlQ{OyEQvF2= MWDTRW5ITVJ?
D-247MG MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3f1eWlEPTB;Mz64OlA{OSEQvF2= NUXDe|NNW0GQR1XS
COLO-320-HSR MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4eyd2lEPTB;Mz65NVI4OSEQvF2= MnnwV2FPT0WU
A253 MonLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDUd4xnUUN3ME2zMlkyPDN4IN88US=> MX;TRW5ITVJ?
HT55 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3f0SmlEPTB;Mz65NVc4OyEQvF2= MU\TRW5ITVJ?
MZ1-PC NXHVenE6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\qPGlEPTB;Mz65OVg3PCEQvF2= MnTXV2FPT0WU
RERF-LC-MS MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTNwOU[1NlUh|ryP NEX2[5pUSU6JRWK=
SF268 MofkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2C4eWlEPTB;ND6xNlg5OSEQvF2= MYrTRW5ITVJ?
RMG-I NHPXNZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTWRmJKSzVyPUSuNlE1PTRizszN MWXTRW5ITVJ?
CFPAC-1 Mk\vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HXXWlEPTB;ND6yNVY5PSEQvF2= M1jGTnNCVkeHUh?=
S-117 NWHSOY9MT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnlZlI3UUN3ME20MlIzODJ2IN88US=> NEHYV4dUSU6JRWK=
NCI-H2122 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkixTWM2OD12LkOwPVMyKM7:TR?= MVfTRW5ITVJ?
J82 M2TQO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzjXHBxUUN3ME20MlM{PDRzIN88US=> NEDFN2lUSU6JRWK=
CAL-72 M{TGOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnI[|JKSzVyPUSuN|U1PzdizszN MXrTRW5ITVJ?
GP5d M1H1OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfUTWM2OD12LkSxNlk5KM7:TR?= MU\TRW5ITVJ?
UACC-62 NFnzdZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\YSGlEPTB;ND60OlU1PSEQvF2= MlPvV2FPT0WU
MS-1 M{HsOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrI[IZKSzVyPUSuOFkyOTVizszN NXfN[2QyW0GQR1XS
RCM-1 MlLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTRwNUK5NkDPxE1? NG\2NmVUSU6JRWK=
IST-SL1 M{nk[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVL6U3BrUUN3ME20MlY2OzV7IN88US=> MlTxV2FPT0WU
SW756 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3L1U2lEPTB;ND62PVE{PSEQvF2= MYDTRW5ITVJ?
HCC38 MkDSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTtU3RKSzVyPUSuO|EyQTdizszN MoLsV2FPT0WU
YAPC M4H2Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\FPGlEPTB;ND63NVMyOyEQvF2= NFfYOXdUSU6JRWK=
DK-MG MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHue2dKSzVyPUSuO|gyOTJizszN NXTZRZlUW0GQR1XS
HCC1937 M1HTcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1fFdGlEPTB;ND64OVEzPSEQvF2= M{HX[HNCVkeHUh?=
OAW-28 MoLMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnDPG1LUUN3ME20MlkzPDN3IN88US=> M{f3VHNCVkeHUh?=
MEG-01 NXfubFdIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVTxb2I6UUN3ME21MlA5OzJ6IN88US=> M1vGZXNCVkeHUh?=
GI-1 NFfy[XRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLFTWM2OD13LkKzOFg3KM7:TR?= M170PHNCVkeHUh?=
FADU MknQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;FPWlEPTB;NT6yO|g{PyEQvF2= NHnPbGZUSU6JRWK=
CAPAN-1 NITmOFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrZTYNVUUN3ME21MlM6PDN{IN88US=> NGHlfZRUSU6JRWK=
TGBC11TKB NEfh[3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTVwNEW3PFYh|ryP NX3UcYJRW0GQR1XS
DBTRG-05MG MoKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTPdFJPUUN3ME21MlUxPTJizszN NWrpfYpwW0GQR1XS
ONS-76 NIizXphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTVwNUW4NFEh|ryP NUXON3lQW0GQR1XS
Ca-Ski NUizWoF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3iTWM2OD13Lk[yOVY3KM7:TR?= MUDTRW5ITVJ?
COLO-741 NIrGW2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHnTWM2OD13LkexPVk2KM7:TR?= NXjWeZA2W0GQR1XS
HT-29 NXfwU4lLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4K2O2lEPTB;NT64NFQ4PSEQvF2= M1jRO3NCVkeHUh?=
HCC1187 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PTPGlEPTB;NT64NVAxQCEQvF2= M2nmdnNCVkeHUh?=
Capan-2 M4HKUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGTScYNKSzVyPUWuPVU6QTRizszN M1LTdXNCVkeHUh?=
NCI-H630 MojrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3ja[mlEPTB;Nj6xNVQ1OSEQvF2= NFPS[lVUSU6JRWK=
COLO-678 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3K2PWlEPTB;Nj6xOVA6OiEQvF2= MoTtV2FPT0WU
D-502MG MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXxboFUUUN3ME22MlE4OzN4IN88US=> MlTYV2FPT0WU
NB17 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTZwMkS5OFMh|ryP MWjTRW5ITVJ?
SK-MEL-24 M3jT[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7xTWM2OD14LkK2OFkh|ryP MWDTRW5ITVJ?
NCI-SNU-5 NUT6OXdkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NECxNnBKSzVyPU[uNlcyOThizszN M4TZXXNCVkeHUh?=
D-392MG MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlW3TWM2OD14LkOzN|k6KM7:TR?= Mm\2V2FPT0WU
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293T  MmDOSpVv[3Srb36gRZN{[Xl? MWOyNEBvVQ>? NWm4dmV7OyCq MXjkbZNzfXC2czDBb5QudWWmaXH0[YQhXEmILVnBJJN1[WKrbHn0fUwhfHKjboPsc4NifGmxbjygZY5lKGGldHn2bZR6 NFXo[IYzPDN4M{S0PS=>
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Hep-2 M3rJNWZ2dmO2aX;uJGF{e2G7 NETDRm4xNjFxMD61JO69VQ>? M32zc|I1KGh? MoixZoxw[2u|IH3UU3JEOSCjbnSgcXRQWkN{IHHjeIl3[XSrb36= MnzPNlQyODN5NEm=
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BT-12 NEnITFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXztRlhXUUN3ME2yOE44KG6P MV2yNVM{PzZ5OR?=
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TC-71 NVX3d29vT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nRTmlEPTB;NE[uOUBvVQ>? MknONlE{Ozd4N{m=
CHLA-9 NVX1SGNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHWTWM2OD1zNT6zJI5O NW\LN2s{OjF|M{e2O|k>
CHLA-10 MoLYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTNwOTDuUS=> NV\xdmdCOjF|M{e2O|k>
CHLA-258 NWPy[ZhJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3HUVWlEPTB;MUCuOEBvVQ>? NX7KRWQ6OjF|M{e2O|k>
GBM2 NFyxN|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTWbpQ4UUN3ME20NE44KG6P NIX3TpczOTN|N{[3PS=>
NB-1643 M{WzWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3aVFBKSzVyPUOzMlghdk1? MmSzNlE{Ozd4N{m=
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CHLA-90 NELsbphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTN2LkWgcm0> M1;G[|IyOzN5Nke5
CHLA-136 NFK4cVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPielduUUN3ME2xOlIvPiCwTR?= MoW5NlE{Ozd4N{m=
NALM-6 NILUXYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3Lld2lEPTB;M{KuPUBvVQ>? MnXQNlE{Ozd4N{m=
COG-LL-317 M4ruSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPEUmpOUUN3ME2xMlMhdk1? MkTMNlE{Ozd4N{m=
RS4;11 M3HkNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkGyTWM2OD1|NTDuUS=> Mmf6NlE{Ozd4N{m=
MOLT-4 NUXscVJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRThwNjDuUS=> M4fMflIyOzN5Nke5
CCRF-CEM MlTnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTRwMTDuUS=> NV;mOFIxOjF|M{e2O|k>
Kasumi-1 M1[1T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTFwNDDuUS=> MX:yNVM{PzZ5OR?=
Karpas-299 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTDTWM2OD1{MT63JI5O MlTSNlE{Ozd4N{m=
Ramos-RA1 NHPzZWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4P4SmlEPTB;OD6yJI5O NW\MdppnOjF|M{e2O|k>
H838 NF7OTmZHfW6ldHnvckBCe3OjeR?= MWmxJO69VQ>? NFT1WlQ1QCCq NGDCZ21qdmS3Y3XzJINmdGxiYYX0c5Bp[We7 MUWyNFM3PDFzMx?=
H838 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;3NE0{KM7:TR?= NHiwSnA4OiCq NY\DdI5KcW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 NXTD[mVWOjByMki4OVQ>
A549  NGDCSYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1vBSVAuOyEQvF2= Ml\LO|IhcA>? M{LKT4lv\HWlZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKGG3dH;wbIFogQ>? NUXEbVc1OjByMki4OVQ>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; 

PubMed: 24457069     


Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙

p-EGFR / EGFR; 

PubMed: 25654224     


PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot;

p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; 

PubMed: 25654224     


PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.

24457069 25654224
Immunofluorescence
p-4EBP1 / EIF4E / mTOR; 

PubMed: 24600487     


(C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋€𢡄�෋€䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemセ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒セ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wセ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v 玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴￿￿嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗

LC3B / p62 ; 

PubMed: 30337536     


Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞

24600487 30337536
Growth inhibition assay
Cell viability ; 

PubMed: 29263324     


Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.

29263324
In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
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Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
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  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Water Insoluble
Ethanol '3 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A
Smiles COC1=C(CO)C=C(C=C1)C2=NC3=C(C=C2)C(=NC(=N3)N4CCOCC4C)N5CCOCC5C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O
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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID