AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
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Cited by 113 Publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 M2fZ[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGTudW5KSzVyPUCuNFI{OzFizszN NYPJSYtRW0GQR1XS
NCI-H720 NUTGOmdzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkX3TWM2OD1yLkCyPVM5KM7:TR?= M2r5XnNCVkeHUh?=
KARPAS-45 M3vWR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzQPW5KSzVyPUCuNFM{PzFizszN NFrVb4NUSU6JRWK=
EW-3 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTBwMESwOVYh|ryP NGXtXJlUSU6JRWK=
SBC-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPiVmtWUUN3ME2wMlA1PThzIN88US=> NVjHbJF1W0GQR1XS
NCI-H209 NWTNR49qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\rWVNKSzVyPUCuNFU1PTNizszN M3viSnNCVkeHUh?=
EoL-1-cell M3TBVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFz3eXJKSzVyPUCuNFY2PjlizszN NFvkXZJUSU6JRWK=
H9 M3zuSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTBwME[2PVIh|ryP NXX6bmFOW0GQR1XS
AN3-CA MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFn6PHpKSzVyPUCuNFc6PTFizszN MW\TRW5ITVJ?
HH NYjQTZJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMEiwNFkh|ryP MnzZV2FPT0WU
KU812 MkjHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPSW4lKSzVyPUCuNFgxPDNizszN NITheWVUSU6JRWK=
DU-4475 MofrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV32TppUUUN3ME2wMlA5ODR5IN88US=> MmDTV2FPT0WU
CTV-1 NFn6fFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jBXGlEPTB;MD6wPFY1OSEQvF2= Mm\JV2FPT0WU
BV-173 NYrINpJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\RNlNKSzVyPUCuNFkxPjFizszN MUfTRW5ITVJ?
ES5 Mlu5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjCZY5nUUN3ME2wMlA6OzVizszN MWjTRW5ITVJ?
G-402 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIKxeZNKSzVyPUCuNFk6QCEQvF2= MXrTRW5ITVJ?
MV-4-11 Mk[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULxSndLUUN3ME2wMlExOzR5IN88US=> M{TYfXNCVkeHUh?=
NB69 NGq3bodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTBwMUO1JO69VQ>? MoPjV2FPT0WU
EGI-1 NWHKb|hNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnZXJpKSzVyPUCuNVM2OzFizszN MULTRW5ITVJ?
EW-18 NFzxT5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvUZnVKUUN3ME2wMlE1ODd|IN88US=> M4fZd3NCVkeHUh?=
ME-180 NY\SWYtUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjk[5F1UUN3ME2wMlE1PjhzIN88US=> MXzTRW5ITVJ?
D-283MED M2C1eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7mbHRvUUN3ME2wMlE1PzJ|IN88US=> M4DZS3NCVkeHUh?=
NB14 M2fKXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfxOldKSzVyPUCuNVY1PDdizszN NYTwTY5kW0GQR1XS
Ramos-2G6-4C10 M3;BTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nWV2lEPTB;MD6xOlk5OyEQvF2= NX:zW5M1W0GQR1XS
NBsusSR M{Prdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTBwMUewNVkh|ryP NHHKbHRUSU6JRWK=
LAMA-84 NYjwZ|lNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfnRpBKSzVyPUCuNVgxOzJizszN M2DNSXNCVkeHUh?=
NCI-H1581 NYPhelJ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTBwMUi5OlYh|ryP NXTH[mFEW0GQR1XS
A204 NGH1cmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXpTWM2OD1yLkG4PVch|ryP MWTTRW5ITVJ?
A549 NXP5flJ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;WTWM2OD1yLkG5OVUh|ryP M17kSXNCVkeHUh?=
CAL-51 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH31T|dKSzVyPUCuNVk3PTFizszN NXuyV3QyW0GQR1XS
NKM-1 Mmq5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELIPXlKSzVyPUCuNlAxOjlizszN NIHxR5dUSU6JRWK=
LoVo MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTBwMkCyNFIh|ryP NGnveo1USU6JRWK=
HAL-01 NInyW4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NED1epVKSzVyPUCuNlAzQTdizszN NXvVcZlPW0GQR1XS
HCC2218 NX;6OodpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\Jd2lEPTB;MD6yNFY5QCEQvF2= NFvCXIdUSU6JRWK=
BEN NWqxW5RbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDsNo5KSzVyPUCuNlEzQTNizszN MlLaV2FPT0WU
LC-2-ad NVr6O2g1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTCS5JJUUN3ME2wMlIzODh2IN88US=> NEW0bYdUSU6JRWK=
LXF-289 M4jYWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTBwMkWwOFEh|ryP MkTzV2FPT0WU
HGC-27 MmPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn2wTWM2OD1yLkK2OFE1KM7:TR?= MYDTRW5ITVJ?
LK-2 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLWdlhiUUN3ME2wMlI3QDJ2IN88US=> MkLJV2FPT0WU
CTB-1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\ocWlEPTB;MD6yO|IyPSEQvF2= NYnWdoVTW0GQR1XS
697 M{P2cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jBVWlEPTB;MD6yO|M3PyEQvF2= Mn3QV2FPT0WU
ES7 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLITWM2OD1yLkK3N|g{KM7:TR?= MV\TRW5ITVJ?
MG-63 Mn;nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnn0TWM2OD1yLkK3OFA4KM7:TR?= NV\SV4hDW0GQR1XS
T47D MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTBwMke0O|Uh|ryP NIX6WIVUSU6JRWK=
RPMI-8226 NVjacW42T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrlRlVKSzVyPUCuNlg2QDlizszN MoLLV2FPT0WU
NCI-H292 Mn\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTBwMkmxOlQh|ryP NYHxRVE6W0GQR1XS
HT NH7QZXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYr4UHgxUUN3ME2wMlI6OzhizszN M4XxXHNCVkeHUh?=
A3-KAW NHL3TXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTBwMkm3OEDPxE1? MVXTRW5ITVJ?
LB2241-RCC M4\rRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{DoemlEPTB;MD6zNFE6QCEQvF2= NGHQU5hUSU6JRWK=
BB65-RCC M2j2TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWP3[pNWUUN3ME2wMlMxQDB3IN88US=> MnTXV2FPT0WU
G-401 NYnBeWNwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXqOXdKSzVyPUCuN|A6QDZizszN MUfTRW5ITVJ?
TYK-nu NGnJNWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTwcVVKSzVyPUCuN|E5PjRizszN M1nE[XNCVkeHUh?=
NCI-SNU-1 Mom3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml62TWM2OD1yLkOyN|MyKM7:TR?= NEexO4pUSU6JRWK=
CHL-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXad5ptUUN3ME2wMlMzPzB|IN88US=> M1XKd3NCVkeHUh?=
P30-OHK NWi3[IxuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\6eGlEPTB;MD6zNlcxPSEQvF2= MlzVV2FPT0WU
PA-1 M4\ORmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTBwM{O2NVkh|ryP MUnTRW5ITVJ?
LB1047-RCC Mof2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPBVphKSzVyPUCuN|QxPDdizszN MlfiV2FPT0WU
EM-2 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvWTWM2OD1yLkO0NVg5KM7:TR?= MlnuV2FPT0WU
VA-ES-BJ NEnpeHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jUdGlEPTB;MD6zOFI3QCEQvF2= MmTjV2FPT0WU
NCI-H520 NXO4PZFIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mkf0TWM2OD1yLkO0PFM1KM7:TR?= MojXV2FPT0WU
ECC10 NXLscFA1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjW[ng3UUN3ME2wMlM2ODN3IN88US=> NVrtS4RjW0GQR1XS
LNCaP-Clone-FGC NX;ycHdtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknCTWM2OD1yLkO1N|E1KM7:TR?= NH\rSolUSU6JRWK=
ES8 MnS0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DYOGlEPTB;MD6zOlU2OyEQvF2= NIe2VlJUSU6JRWK=
HuO-3N1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjmeZA1UUN3ME2wMlM5Ozd7IN88US=> M3m5UHNCVkeHUh?=
L-363 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLOSZpFUUN3ME2wMlM6ODJ4IN88US=> NYG2Nnl6W0GQR1XS
SK-LU-1 NHLFWJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTBwM{myNFgh|ryP MonWV2FPT0WU
MCF7 NFH2RXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PTRWlEPTB;MD60NFU{KM7:TR?= M{XvZ3NCVkeHUh?=
HuO9 NH65cWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvuNpppUUN3ME2wMlQxPjZ5IN88US=> MVTTRW5ITVJ?
BHT-101 NEjDeIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrs[WZGUUN3ME2wMlQxPzh5IN88US=> NWK1NXFzW0GQR1XS
MZ2-MEL M2G4PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLjZYY{UUN3ME2wMlQxQDh{IN88US=> NHLreWhUSU6JRWK=
NOS-1 Mnz3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4j4[GlEPTB;MD60NVA4PCEQvF2= NFPR[o5USU6JRWK=
NEC8 MnTqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWPZOXpsUUN3ME2wMlQyPjl3IN88US=> NVjod2tsW0GQR1XS
COR-L88 Mlr3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rGR2lEPTB;MD60NlI3PyEQvF2= MoS4V2FPT0WU
MFM-223 NUfTfY9wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;NTWM2OD1yLkSyOlE4KM7:TR?= NVnhTpNGW0GQR1XS
HSC-3 MnzOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrFTWM2OD1yLkSyPVA5KM7:TR?= NEjCXGRUSU6JRWK=
OVCAR-5 NUfQUWt3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnuSWRrUUN3ME2wMlQzQTN|IN88US=> NXPD[Wd3W0GQR1XS
MFE-280 NY\pTXd6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3qwOmlEPTB;MD60N|A{OiEQvF2= M2L4cXNCVkeHUh?=
KP-N-YS MoHuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3PdVZKSzVyPUCuOFMxPyEQvF2= M3P2fHNCVkeHUh?=
EPLC-272H MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfvRYhPUUN3ME2wMlQ{ODd5IN88US=> MYrTRW5ITVJ?
KE-37 NWGzbHBrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTBwNEOyOFch|ryP M4riZnNCVkeHUh?=
HuH-7 MmHOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLKTWM2OD1yLkSzN|czKM7:TR?= M2LJS3NCVkeHUh?=
CHP-212 M33JSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2HqPGlEPTB;MD60N|Y6PyEQvF2= MlrXV2FPT0WU
SK-MES-1 M1nwVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnG3TWM2OD1yLkSzPFQ5KM7:TR?= NIf5XYNUSU6JRWK=
SJSA-1 Mmi0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{nvXGlEPTB;MD60OFU2PCEQvF2= NGT5RmFUSU6JRWK=
QIMR-WIL MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXSdZdKSzVyPUCuOFUyPSEQvF2= NHjXPHFUSU6JRWK=
ES4 NW\KOJl[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmn0TWM2OD1yLkS1N|M3KM7:TR?= NV7tV5NmW0GQR1XS
TI-73 NYjnWpJ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\lTWM2OD1yLkS1Olg3KM7:TR?= NVzkV|FGW0GQR1XS
COLO-684 M1PTd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvkXGJKSzVyPUCuOFU4OjZizszN NXTDO3lZW0GQR1XS
GCIY NXzubVBGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLETWM2OD1yLkS1O|U1KM7:TR?= MV7TRW5ITVJ?
JVM-3 NWezXG5kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTBwNE[yPVIh|ryP NHPselBUSU6JRWK=
MPP-89 NVPEPIhHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PpUWlEPTB;MD60Olk1PyEQvF2= MnnmV2FPT0WU
M14 NHXiU3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3i2dGlEPTB;MD60O|E4KM7:TR?= M2nkSHNCVkeHUh?=
SW962 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTBwNEexPUDPxE1? M3HWO3NCVkeHUh?=
VM-CUB-1 NWn5OpE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXN[|doUUN3ME2wMlQ4Ojh4IN88US=> NXLlZ2lNW0GQR1XS
A101D NYPyUGRtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DzPWlEPTB;MD60PFk3QCEQvF2= MXrTRW5ITVJ?
DB M1jn[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1G3ZmlEPTB;MD61NFAzOSEQvF2= MmD1V2FPT0WU
KNS-62 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzqWHNKSzVyPUCuOVA2PjhizszN M16yenNCVkeHUh?=
NCI-H460 M1:2OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzO[lNKSzVyPUCuOVA2QTdizszN NE\VVWpUSU6JRWK=
MDA-MB-361 NWLG[oJlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LLVGlEPTB;MD61NFg4KM7:TR?= NH3TN49USU6JRWK=
DOK Mmn5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWixc41sUUN3ME2wMlUyOzN|IN88US=> Mlf5V2FPT0WU
CAL-39 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml[4TWM2OD1yLkWyNlY5KM7:TR?= NFW2epdUSU6JRWK=
HT-1080 NGrOcXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPETWM2OD1yLkWyOVQ{KM7:TR?= M3zafnNCVkeHUh?=
GR-ST M1zhdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\1XpltUUN3ME2wMlU{ODF3IN88US=> MUnTRW5ITVJ?
J-RT3-T3-5 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHSXVVKSzVyPUCuOVMyQTVizszN MoWyV2FPT0WU
NCI-H1793 NU\keYdGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vjcWlEPTB;MD61N|Q1PyEQvF2= NVP5T5l7W0GQR1XS
NCI-H1355 M1nCXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;EfnVLUUN3ME2wMlU{QDd6IN88US=> MkLBV2FPT0WU
YH-13 Ml6wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MorWTWM2OD1yLkW0PVM5KM7:TR?= MU\TRW5ITVJ?
LOXIMVI NHHufJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTBwNUWxNlMh|ryP NI[wd4xUSU6JRWK=
ES1 NGO5blVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvXbWZKSzVyPUCuOVU2OThizszN NYr3XJZoW0GQR1XS
GAMG MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljjTWM2OD1yLkW1OlU5KM7:TR?= NXHMSJl6W0GQR1XS
SK-MEL-28 NGG0PJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTBwNUW5NUDPxE1? NX;Sdo8{W0GQR1XS
KG-1 MnvjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTBwNU[4PFQh|ryP NIXldGlUSU6JRWK=
HOS NGfSdY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTBwNUexPVIh|ryP M4Xod3NCVkeHUh?=
PANC-08-13 MlK0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;sVZFzUUN3ME2wMlU4Ozh7IN88US=> NHjaOVZUSU6JRWK=
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NCI-H1693 NWCwVHlyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTVTWM2OD1zLkm4NFg3KM7:TR?= MVzTRW5ITVJ?
8-MG-BA NHPFdG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk[0TWM2OD1zLkm4NlEh|ryP NGnae|NUSU6JRWK=
OVCAR-8 M{\EPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjlT2hKSzVyPUKuNFAzQTVizszN MoT4V2FPT0WU
DSH1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\OOZVpUUN3ME2yMlAyPzd2IN88US=> NXjMe4piW0GQR1XS
KYSE-520 M3Kwb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPNcI51UUN3ME2yMlAzODR7IN88US=> NFyyV2dUSU6JRWK=
GB-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvwTWM2OD1{LkCyOVc4KM7:TR?= NXfR[JBwW0GQR1XS
RCC10RGB M2rOdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDFTWM2OD1{LkC0PFcyKM7:TR?= MY\TRW5ITVJ?
SW954 NGO3XpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4L0ZWlEPTB;Mj6wOVA{PiEQvF2= NUjJVZJ4W0GQR1XS
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AU565 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PCcGlEPTB;Mj6wOVkxPSEQvF2= NF\Kc|RUSU6JRWK=
CW-2 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\IUItKSzVyPUKuNFY5PzVizszN NYLD[nl5W0GQR1XS
TE-1 NXjq[HN1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWezR|dUUUN3ME2yMlA6Ozd5IN88US=> Mn;YV2FPT0WU
KP-4 MkL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTJwMUCzNVIh|ryP MkPVV2FPT0WU
SNU-C2B NWW1cFh5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTJwMUC4NlMh|ryP NHj6eWdUSU6JRWK=
NCI-H1395 MnrmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTJwMUC5PFYh|ryP NFLZemRUSU6JRWK=
CAS-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4THV2lEPTB;Mj6xNVU1PiEQvF2= M1rhe3NCVkeHUh?=
NCI-H1155 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPoTWM2OD1{LkKyOlkh|ryP NYrlbpM1W0GQR1XS
SK-MEL-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjIXJhuUUN3ME2yMlI5OThzIN88US=> Mn;2V2FPT0WU
KINGS-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXu5dlJpUUN3ME2yMlMxODh6IN88US=> MnXpV2FPT0WU
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HT-1376 Ml;wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vRNWlEPTB;Mj6zPFcxQCEQvF2= NIrPbItUSU6JRWK=
LS-513 MnXuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fJdGlEPTB;Mj6zPFg4PCEQvF2= M{jqPHNCVkeHUh?=
KNS-42 NEL4c|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;IWnFKSzVyPUKuN|kyODVizszN MkTkV2FPT0WU
639-V M3\HdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XMZWlEPTB;Mj60Nlc2QCEQvF2= NGHo[VVUSU6JRWK=
SJRH30 NICzW4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVO1[ZpWUUN3ME2yMlQ2OTZzIN88US=> MmG0V2FPT0WU
SW1783 MmPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzkTWM2OD1{LkS3NFc5KM7:TR?= M{HNSXNCVkeHUh?=
NB6 M3nvUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkX1TWM2OD1{LkS4Nlgh|ryP MoT2V2FPT0WU
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HCE-4 M{PkSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3uwdWlEPTB;Mj60PVQxOiEQvF2= M4GwS3NCVkeHUh?=
NB10 NUHsbYZZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NID5c5dKSzVyPUKuOFk1OjlizszN Mo\HV2FPT0WU
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SK-HEP-1 MonwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LCdmlEPTB;Mj61OVI{PiEQvF2= NUj6cXlbW0GQR1XS
HCC1395 NVPL[ldZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmToTWM2OD1{LkW1OlU2KM7:TR?= M{PUWnNCVkeHUh?=
HuCCT1 Ml;SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2CxcWlEPTB;Mj61OlM6KM7:TR?= NEXpUJpUSU6JRWK=
SiHa MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvKTWM2OD1{LkW4O|E6KM7:TR?= MXXTRW5ITVJ?
EFO-21 MnywS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTJwNUi3OlQh|ryP M{nZb3NCVkeHUh?=
NCI-H1666 NILtSIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTJwNUmwO|Yh|ryP M4izZnNCVkeHUh?=
UMC-11 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWm4XI93UUN3ME2yMlU6QDZ6IN88US=> MXHTRW5ITVJ?
COLO-679 NFvMSGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXzZZJTUUN3ME2yMlYyOjN|IN88US=> MXPTRW5ITVJ?
A388 NVnZcINCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3UN5M2UUN3ME2yMlY2OjV|IN88US=> NUj2e41sW0GQR1XS
LB2518-MEL MlP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzzRpBKUUN3ME2yMlY2PDB6IN88US=> NXv3[GNxW0GQR1XS
EW-13 Mk\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXlb25KSzVyPUKuOlg4PjhizszN MXnTRW5ITVJ?
MLMA MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTJwNki4OVYh|ryP M2TERXNCVkeHUh?=
HOP-92 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHNTWM2OD1{LkewNVEh|ryP MU\TRW5ITVJ?
NOMO-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3TCdmlEPTB;Mj63NVQ1OiEQvF2= NFPWZmlUSU6JRWK=
NCI-H810 MkPwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTXcpV[UUN3ME2yMlczOTl6IN88US=> Mn7NV2FPT0WU
769-P MnPZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3frcmlEPTB;Mj63NlI5KM7:TR?= MlPjV2FPT0WU
SAS NEG2R2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M371SWlEPTB;Mj63OlUh|ryP M3rHe3NCVkeHUh?=
M059J NW\QSmZzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTJwN{izNVgh|ryP NUPZRXdKW0GQR1XS
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GT3TKB NGC4XWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4i3ZmlEPTB;Mj64N|Q{OyEQvF2= M3nLcnNCVkeHUh?=
MZ7-mel M2rhT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITpXXlKSzVyPUKuPFY2ODJizszN MU\TRW5ITVJ?
KYSE-510 M{XkTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTJwOEm1NFQh|ryP M3XifnNCVkeHUh?=
NCI-N87 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTJwOUG4NFch|ryP M1nYN3NCVkeHUh?=
MKN1 MmDvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWPZ[JZFUUN3ME2zMlAxOTZ|IN88US=> MYLTRW5ITVJ?
NCI-H2170 NFXVSpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHqT3I{UUN3ME2zMlAxPDVzIN88US=> MYLTRW5ITVJ?
OVCAR-3 NGXUO|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTNwMEKxNVUh|ryP Ml31V2FPT0WU
KLE NYG2XodnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2C1cmlEPTB;Mz6wOlgyOiEQvF2= M4jzTXNCVkeHUh?=
LB771-HNC NYfmc2RzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFOyTmRKSzVyPUOuNFk{PDNizszN NXXST|F5W0GQR1XS
MC116 NWiwbYZST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLRbI5tUUN3ME2zMlEyOTF{IN88US=> NH7vdYpUSU6JRWK=
SCC-9 NVHpVFU2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnDTWM2OD1|LkGxN|Y3KM7:TR?= MXLTRW5ITVJ?
U031 MoLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTNwMUK0NlMh|ryP M2Dz[HNCVkeHUh?=
LCLC-103H MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTNwMkO4PVQh|ryP NXr3XmxpW0GQR1XS
U-266 MlHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4mz[mlEPTB;Mz6yO|Y{OyEQvF2= MXzTRW5ITVJ?
JVM-2 MlqxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTNwM{KxOlQh|ryP MoHyV2FPT0WU
UACC-257 NF:4SHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTNwNE[0N|Eh|ryP MlXZV2FPT0WU
LB996-RCC NITIbXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTNwNE[1NFIh|ryP M{fyW3NCVkeHUh?=
BT-549 NEKzZ3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPQWHNZUUN3ME2zMlUxOjB7IN88US=> MVfTRW5ITVJ?
NCI-H526 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHiTWM2OD1|LkW0NVU5KM7:TR?= NGHFfJVUSU6JRWK=
RH-18 MlvYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M13SXGlEPTB;Mz62NVc6QCEQvF2= NGCxSlVUSU6JRWK=
SW626 Mnv5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2TTUmlEPTB;Mz63NVEzQCEQvF2= MojZV2FPT0WU
SK-N-AS Ml\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUT0T3RRUUN3ME2zMlc{OjR5IN88US=> M1\nc3NCVkeHUh?=
IGR-1 NXPvbWZZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XGW2lEPTB;Mz63OFI1QCEQvF2= M2f3cXNCVkeHUh?=
NCI-H2291 M2TPWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTNwN{WzN|Yh|ryP M4\MdXNCVkeHUh?=
NCI-H441 NXfLN3lUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\oTWM2OD1|LkiyOFM{KM7:TR?= M3LSfHNCVkeHUh?=
D-247MG NHP0e49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3S0V2lEPTB;Mz64OlA{OSEQvF2= MWLTRW5ITVJ?
COLO-320-HSR NYDLdYhQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\UZmlEPTB;Mz65NVI4OSEQvF2= NXTC[|l[W0GQR1XS
A253 M1\ZXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7tO2lKSzVyPUOuPVE1OzZizszN M4XNSHNCVkeHUh?=
HT55 Mn;ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVq5UpNJUUN3ME2zMlkyPzd|IN88US=> NUfwOYtjW0GQR1XS
MZ1-PC MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLqTWM2OD1|Lkm1PFY1KM7:TR?= M3\CW3NCVkeHUh?=
RERF-LC-MS NX;NR4J3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmeyTWM2OD1|Lkm2OVI2KM7:TR?= MmTpV2FPT0WU
SF268 MlvlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2KxOGlEPTB;ND6xNlg5OSEQvF2= M2PLZnNCVkeHUh?=
RMG-I MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTiXYJKSzVyPUSuNlE1PTRizszN MXfTRW5ITVJ?
CFPAC-1 NIGxZXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HpTmlEPTB;ND6yNVY5PSEQvF2= NWLYdHJ6W0GQR1XS
S-117 NE\1V2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFj3dJZKSzVyPUSuNlIxOjRizszN MVXTRW5ITVJ?
NCI-H2122 Mo\yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXYb5ZkUUN3ME20MlMxQTNzIN88US=> MUjTRW5ITVJ?
J82 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;1XmlEPTB;ND6zN|Q1OSEQvF2= NYjyTW1MW0GQR1XS
CAL-72 M2LsbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jsPWlEPTB;ND6zOVQ4PyEQvF2= NFr4SG5USU6JRWK=
GP5d NG\iPXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYThWmdFUUN3ME20MlQyOjl6IN88US=> NFqzTFlUSU6JRWK=
UACC-62 MmTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTHfZNqUUN3ME20MlQ3PTR3IN88US=> NXnHUoc1W0GQR1XS
MS-1 MoPTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfwSHlKSzVyPUSuOFkyOTVizszN M3;MOHNCVkeHUh?=
RCM-1 NFS1eHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTRwNUK5NkDPxE1? MWnTRW5ITVJ?
IST-SL1 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH3NfnRKSzVyPUSuOlU{PTlizszN MnXoV2FPT0WU
SW756 NXex[mdET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XjT2lEPTB;ND62PVE{PSEQvF2= MmjoV2FPT0WU
HCC38 MlLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4XTfGlEPTB;ND63NVE6PyEQvF2= MmjFV2FPT0WU
YAPC MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnuVZVKSzVyPUSuO|E{OTNizszN M4nV[HNCVkeHUh?=
DK-MG M3zZWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTRwN{ixNVIh|ryP NH7rUnpUSU6JRWK=
HCC1937 Ml3DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\IW49KSzVyPUSuPFUyOjVizszN NITUT29USU6JRWK=
OAW-28 NHPjNGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPOUWlKSzVyPUSuPVI1OzVizszN MXzTRW5ITVJ?
MEG-01 M2W5T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLkTWM2OD13LkC4N|I5KM7:TR?= M1PnZnNCVkeHUh?=
GI-1 NYHQc2JDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTVwMkO0PFYh|ryP MY\TRW5ITVJ?
FADU NILBPIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTVwMke4N|ch|ryP MkP3V2FPT0WU
CAPAN-1 M4LzOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DvSGlEPTB;NT6zPVQ{OiEQvF2= MnPPV2FPT0WU
TGBC11TKB MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTVwNEW3PFYh|ryP NVPWeVFwW0GQR1XS
DBTRG-05MG MnvOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\FPHNKSzVyPUWuOVA2OiEQvF2= MoXTV2FPT0WU
ONS-76 Mn;vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTVwNUW4NFEh|ryP Mke3V2FPT0WU
Ca-Ski MnmwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\YWmlEPTB;NT62NlU3PiEQvF2= M3rpPXNCVkeHUh?=
COLO-741 MkC2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7ZNIw5UUN3ME21MlcyQTl3IN88US=> MYrTRW5ITVJ?
HT-29 NHrLdodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDjTWM2OD13LkiwOFc2KM7:TR?= NWHoSpVwW0GQR1XS
HCC1187 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFWzRXdKSzVyPUWuPFExODhizszN Ml7IV2FPT0WU
Capan-2 MnrWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHuzeWlKSzVyPUWuPVU6QTRizszN NXK0clc5W0GQR1XS
NCI-H630 NX;WZ|I5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHqzWHJKSzVyPU[uNVE1PDFizszN MXvTRW5ITVJ?
COLO-678 NEm4dVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnKU3NKSzVyPU[uNVUxQTJizszN Mn7EV2FPT0WU
D-502MG MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\nTWM2OD14LkG3N|M3KM7:TR?= MYPTRW5ITVJ?
NB17 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;MTWM2OD14LkK0PVQ{KM7:TR?= MX3TRW5ITVJ?
SK-MEL-24 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fYPWlEPTB;Nj6yOlQ6KM7:TR?= NX;sO5MxW0GQR1XS
NCI-SNU-5 M3;VeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3NTWM2OD14LkK3NVE5KM7:TR?= MmHaV2FPT0WU
D-392MG Ml;ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHNdXE3UUN3ME22MlM{Ozl7IN88US=> NE\YZXNUSU6JRWK=
A498 NISzVlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTZwNECzNFIh|ryP MWLTRW5ITVJ?
OCI-AML2 M1\lO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWiwXYxUUUN3ME22MlQ2PDR3IN88US=> NFjWcZZUSU6JRWK=
HCC2157 NHPiRpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTZwNEW2NVYh|ryP NILMU2hUSU6JRWK=
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NCI-H1650 MoTnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTZwN{C1NlIh|ryP MU\TRW5ITVJ?
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MOLT-4 NX7CUJJwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3XN|l1UUN3ME24MlYhdk1? NUG3bWJ1OjF|M{e2O|k>
CCRF-CEM NFTYVFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnmSlYzUUN3ME20MlEhdk1? MnT1NlE{Ozd4N{m=
Kasumi-1 NGfu[oFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPle3pKSzVyPUGuOEBvVQ>? MnzlNlE{Ozd4N{m=
Karpas-299 MnfnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnGRoFxUUN3ME2yNU44KG6P MYOyNVM{PzZ5OR?=
Ramos-RA1 NHftXpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3uwTGlEPTB;OD6yJI5O MoLaNlE{Ozd4N{m=
H838 NIHrbYVHfW6ldHnvckBCe3OjeR?= MlLzNUDPxE1? NHvBO3Q1QCCq NXmwcZUxcW6mdXPld{Bk\WyuIHH1eI9xcGGpeR?= MkjQNlA{PjRzMUO=
H838 Ml\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGjrUFIxNTNizszN MX:3NkBp M2LEcolv\HWlZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKGG3dH;wbIFogQ>? NIHnVIYzODB{OEi1OC=>
A549  NV;RdGJoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\TfGIxNTNizszN NXLJUFRvPzJiaB?= NITBRphqdmS3Y3XzJIdzd3e2aDDpcohq[mm2aX;uJIFv\CCjdYTvdIhi\3l? MYCyNFAzQDh3NB?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; 

PubMed: 24457069     


Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙

p-EGFR / EGFR; 

PubMed: 25654224     


PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot;

p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; 

PubMed: 25654224     


PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.

24457069 25654224
Immunofluorescence
p-4EBP1 / EIF4E / mTOR; 

PubMed: 24600487     


(C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋€𢡄�෋€䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemセ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒セ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wセ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v 玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴￿￿嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗

LC3B / p62 ; 

PubMed: 30337536     


Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞

24600487 30337536
Growth inhibition assay
Cell viability ; 

PubMed: 29263324     


Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.

29263324
In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
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Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
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  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID