AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
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Cited by 47 Publications

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;lOIxzUUN3ME2wMlAzOzNzIN88US=> M3r1SHNCVkeHUh?=
NCI-H720 MkXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIC5bW9KSzVyPUCuNFI6OzhizszN MnW2V2FPT0WU
KARPAS-45 Mn\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4TBVmlEPTB;MD6wN|M4OSEQvF2= NYfh[npGW0GQR1XS
EW-3 M2rqOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWfJR|UxRTBwMESwOVYh|ryP M16xd3NCVkeHUh?=
SBC-1 NH23[G1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYntNWZ1UUN3ME2wMlA1PThzIN88US=> NUezWJBNW0GQR1XS
NCI-H209 M1Wzd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjBTWM2OD1yLkC1OFU{KM7:TR?= NYCwWWJWW0GQR1XS
EoL-1-cell MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXUTWM2OD1yLkC2OVY6KM7:TR?= NFLPSlRUSU6JRWK=
H9 MnfwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTBwME[2PVIh|ryP NFG2OFNUSU6JRWK=
AN3-CA NFzOSIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTBwMEe5OVEh|ryP MXzTRW5ITVJ?
HH M1HCNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojaTWM2OD1yLkC4NFA6KM7:TR?= NWLCcnB{W0GQR1XS
KU812 MkfFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TjcWlEPTB;MD6wPFA1OyEQvF2= MnWzV2FPT0WU
DU-4475 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnoUVdxUUN3ME2wMlA5ODR5IN88US=> M1rCfHNCVkeHUh?=
CTV-1 NH7ZPFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTBwMEi2OFEh|ryP M33oWHNCVkeHUh?=
BV-173 NFvQUFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;ONWlEPTB;MD6wPVA3OSEQvF2= NGLJOplUSU6JRWK=
ES5 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\ZcpdKSzVyPUCuNFk{PSEQvF2= MVjTRW5ITVJ?
G-402 NGqzNXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHq5SY1KSzVyPUCuNFk6QCEQvF2= NX74V29GW0GQR1XS
MV-4-11 NFLXOFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPrZpRYUUN3ME2wMlExOzR5IN88US=> MlzOV2FPT0WU
NB69 NF3jbXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2f1UGlEPTB;MD6xN|Uh|ryP MUDTRW5ITVJ?
EGI-1 M4W4[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTBwMUO1N|Eh|ryP M3rM[3NCVkeHUh?=
EW-18 MlfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHfVcJJKSzVyPUCuNVQxPzNizszN Mlr2V2FPT0WU
ME-180 NV\pN|ZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVX2WYViUUN3ME2wMlE1PjhzIN88US=> NF2xc3VUSU6JRWK=
D-283MED NVLMZW9qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7KWGxKSzVyPUCuNVQ4OjNizszN M3vFXnNCVkeHUh?=
NB14 M4LzVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjGbHo1UUN3ME2wMlE3PDR5IN88US=> MkHqV2FPT0WU
Ramos-2G6-4C10 NXXCXpl[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\pU5VKSzVyPUCuNVY6QDNizszN NGj4fXVUSU6JRWK=
NBsusSR NHG5UXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTBwMUewNVkh|ryP Mn3MV2FPT0WU
LAMA-84 NVrn[FBDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnfkTWM2OD1yLkG4NFMzKM7:TR?= MXfTRW5ITVJ?
NCI-H1581 NI[wSmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIj5SGhKSzVyPUCuNVg6PjZizszN MWTTRW5ITVJ?
A204 NWj6O|lyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTBwMUi5O{DPxE1? M{[zdXNCVkeHUh?=
A549 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTBwMUm1OUDPxE1? NHyxcmtUSU6JRWK=
CAL-51 M1:1Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1rLbGlEPTB;MD6xPVY2OSEQvF2= NX3hRmZGW0GQR1XS
NKM-1 MkXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fCNmlEPTB;MD6yNFAzQSEQvF2= NH3CN|BUSU6JRWK=
LoVo M4fsVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTBwMkCyNFIh|ryP MXfTRW5ITVJ?
HAL-01 M1LyeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUKzNHNmUUN3ME2wMlIxOjl5IN88US=> NXjjR|FvW0GQR1XS
HCC2218 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTBwMkC2PFgh|ryP NFjR[IlUSU6JRWK=
BEN M2D4TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLITWM2OD1yLkKxNlk{KM7:TR?= NHrhbXVUSU6JRWK=
LC-2-ad NUTqTHJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTBwMkKwPFQh|ryP MUPTRW5ITVJ?
LXF-289 NYq4WJpIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{D6dWlEPTB;MD6yOVA1OSEQvF2= MmHqV2FPT0WU
HGC-27 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zuc2lEPTB;MD6yOlQyPCEQvF2= MmPmV2FPT0WU
LK-2 M2C2[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTBwMk[4NlQh|ryP MonlV2FPT0WU
CTB-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLo[nRKSzVyPUCuNlczOTVizszN NEDENIdUSU6JRWK=
697 M{fGNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTBwMkezOlch|ryP NF\UcphUSU6JRWK=
ES7 NWf1RnRDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTBwMkezPFMh|ryP NWO0bWhWW0GQR1XS
MG-63 NVPscJllT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkX0TWM2OD1yLkK3OFA4KM7:TR?= MoLjV2FPT0WU
T47D MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFrtRWdKSzVyPUCuNlc1PzVizszN MoHyV2FPT0WU
RPMI-8226 M3jtOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2PxfGlEPTB;MD6yPFU5QSEQvF2= NHnuTZpUSU6JRWK=
NCI-H292 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDv[pI1UUN3ME2wMlI6OTZ2IN88US=> NW\pNHU6W0GQR1XS
HT NV64Xnh{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnr[m1KSzVyPUCuNlk{QCEQvF2= NWDWU457W0GQR1XS
A3-KAW Ml;MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;4TWM2OD1yLkK5O|Qh|ryP M{jpXnNCVkeHUh?=
LB2241-RCC M{X2b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnG4TWM2OD1yLkOwNVk5KM7:TR?= NEDDZnVUSU6JRWK=
BB65-RCC MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\O[5ZxUUN3ME2wMlMxQDB3IN88US=> M3zmWnNCVkeHUh?=
G-401 NFiwbHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnuVIxGUUN3ME2wMlMxQTh4IN88US=> M{DDPHNCVkeHUh?=
TYK-nu M{T3XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfxSYpVUUN3ME2wMlMyQDZ2IN88US=> MX;TRW5ITVJ?
NCI-SNU-1 M1\aeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTBwM{KzN|Eh|ryP MYTTRW5ITVJ?
CHL-1 NYniVVR6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33zVGlEPTB;MD6zNlcxOyEQvF2= Mn3uV2FPT0WU
P30-OHK MnfVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUW2TI9qUUN3ME2wMlMzPzB3IN88US=> NUfm[I1GW0GQR1XS
PA-1 MoTrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTBwM{O2NVkh|ryP MUfTRW5ITVJ?
LB1047-RCC MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Xre2lEPTB;MD6zOFA1PyEQvF2= M3nRXXNCVkeHUh?=
EM-2 NXPOUpRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rhbmlEPTB;MD6zOFE5QCEQvF2= MkTNV2FPT0WU
VA-ES-BJ NWrxO|drT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHJTWM2OD1yLkO0NlY5KM7:TR?= M3HYdXNCVkeHUh?=
NCI-H520 NI\zTlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV3DZohKUUN3ME2wMlM1QDN2IN88US=> NFXkRlhUSU6JRWK=
ECC10 M4XHbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTBwM{WwN|Uh|ryP NFTHT|FUSU6JRWK=
LNCaP-Clone-FGC MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7v[HFKSzVyPUCuN|U{OTRizszN NWrIR4R3W0GQR1XS
ES8 M3H4PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zWVmlEPTB;MD6zOlU2OyEQvF2= NVuw[mZKW0GQR1XS
HuO-3N1 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2ezS2lEPTB;MD6zPFM4QSEQvF2= MnnzV2FPT0WU
L-363 NHfqXlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4K5bGlEPTB;MD6zPVAzPiEQvF2= M4SyTHNCVkeHUh?=
SK-LU-1 NXvuNJROT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTqTWM2OD1yLkO5NlA5KM7:TR?= MnixV2FPT0WU
MCF7 NIO2bFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jDb2lEPTB;MD60NFU{KM7:TR?= MWTTRW5ITVJ?
HuO9 NHqwRolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTzT5NuUUN3ME2wMlQxPjZ5IN88US=> MnvDV2FPT0WU
BHT-101 Mne4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfUTWM2OD1yLkSwO|g4KM7:TR?= NEfRdVBUSU6JRWK=
MZ2-MEL MoTyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDtSpNuUUN3ME2wMlQxQDh{IN88US=> Mkn4V2FPT0WU
NOS-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTBwNEGwO|Qh|ryP NWrxcmhpW0GQR1XS
NEC8 Mni2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\5TWM2OD1yLkSxOlk2KM7:TR?= M3zVVXNCVkeHUh?=
COR-L88 NV\SUVFuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXT3PYY5UUN3ME2wMlQzOjZ5IN88US=> MnXSV2FPT0WU
MFM-223 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3rLS2lEPTB;MD60NlYyPyEQvF2= NELHfIhUSU6JRWK=
HSC-3 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PyN2lEPTB;MD60NlkxQCEQvF2= NUXH[5JvW0GQR1XS
OVCAR-5 MmjnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PBUGlEPTB;MD60Nlk{OyEQvF2= MmrRV2FPT0WU
MFE-280 NIKzdllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHmd3oyUUN3ME2wMlQ{ODN{IN88US=> NHTWZ2VUSU6JRWK=
KP-N-YS MkDrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rMTGlEPTB;MD60N|A4KM7:TR?= NHfHZm5USU6JRWK=
EPLC-272H NX72eWVNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4frXWlEPTB;MD60N|A4PyEQvF2= NYXnUHZ6W0GQR1XS
KE-37 MnziS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLzTWM2OD1yLkSzNlQ4KM7:TR?= NIe4VphUSU6JRWK=
HuH-7 Mn7ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPyV2hnUUN3ME2wMlQ{Ozd{IN88US=> NWf6R49wW0GQR1XS
CHP-212 NV3qe4QyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWfheoNzUUN3ME2wMlQ{Pjl5IN88US=> M{\iTHNCVkeHUh?=
SK-MES-1 M1XGfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHXTWM2OD1yLkSzPFQ5KM7:TR?= MVzTRW5ITVJ?
SJSA-1 NXHuR2VPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTBwNES1OVQh|ryP M4qwbHNCVkeHUh?=
QIMR-WIL NVrsT4tnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\4WWNKSzVyPUCuOFUyPSEQvF2= M3fZOXNCVkeHUh?=
ES4 NWjjRXBHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTBwNEWzN|Yh|ryP M33Id3NCVkeHUh?=
TI-73 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vPOmlEPTB;MD60OVY5PiEQvF2= MVPTRW5ITVJ?
COLO-684 NGS1VFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37BeWlEPTB;MD60OVczPiEQvF2= NWjC[oY{W0GQR1XS
GCIY NEHkXGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHlTWM2OD1yLkS1O|U1KM7:TR?= NYLzTWJXW0GQR1XS
JVM-3 NFrad21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWr5cJFvUUN3ME2wMlQ3Ojl{IN88US=> NIL1VHRUSU6JRWK=
MPP-89 M3jOV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX:zOnJnUUN3ME2wMlQ3QTR5IN88US=> NWC0U5B{W0GQR1XS
M14 M3fTbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXUbGdKSzVyPUCuOFcyPyEQvF2= MWrTRW5ITVJ?
SW962 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonNTWM2OD1yLkS3NVkh|ryP M3zp[XNCVkeHUh?=
VM-CUB-1 NX:4XoJKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jiPWlEPTB;MD60O|I5PiEQvF2= MVnTRW5ITVJ?
A101D M1T3WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYH1dldGUUN3ME2wMlQ5QTZ6IN88US=> NX7FToR6W0GQR1XS
DB NXXDXlJpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTBwNUCwNlEh|ryP NF;DfINUSU6JRWK=
KNS-62 NXP2XWJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTBwNUC1Olgh|ryP NWrtTJN[W0GQR1XS
NCI-H460 NXzmTpV5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTBwNUC1PVch|ryP MVXTRW5ITVJ?
MDA-MB-361 MkXKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;zcHdFUUN3ME2wMlUxQDdizszN NFvC[2FUSU6JRWK=
DOK NFvufYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTBwNUGzN|Mh|ryP MnjRV2FPT0WU
CAL-39 M2CxNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7tUm9KSzVyPUCuOVIzPjhizszN NIG4UYZUSU6JRWK=
HT-1080 NYO2[WxHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVX0Z|lmUUN3ME2wMlUzPTR|IN88US=> NX\3OIFMW0GQR1XS
GR-ST NHHNSnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1e5XGlEPTB;MD61N|AyPSEQvF2= MYfTRW5ITVJ?
J-RT3-T3-5 M{PKb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zrVGlEPTB;MD61N|E6PSEQvF2= MUPTRW5ITVJ?
NCI-H1793 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTBwNUO0OFch|ryP NH3lc2hUSU6JRWK=
NCI-H1355 NIDDcW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnG3TWM2OD1yLkWzPFc5KM7:TR?= MnfKV2FPT0WU
YH-13 M2nFWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwNUS5N|gh|ryP NX;DbFdjW0GQR1XS
LOXIMVI MmLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTBwNUWxNlMh|ryP NY\kXGJmW0GQR1XS
ES1 NEPnWIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mki1TWM2OD1yLkW1OVE5KM7:TR?= MYLTRW5ITVJ?
GAMG MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFK0SmJKSzVyPUCuOVU3PThizszN M4jYXXNCVkeHUh?=
SK-MEL-28 NIjJO3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTBwNUW5NUDPxE1? NX74S4w3W0GQR1XS
KG-1 NX:zXFZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjt[291UUN3ME2wMlU3QDh2IN88US=> MoPlV2FPT0WU
HOS M3vtRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnlNlF2UUN3ME2wMlU4OTl{IN88US=> NUfXNHlFW0GQR1XS
PANC-08-13 NYPwSGh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnDfHV7UUN3ME2wMlU4Ozh7IN88US=> MYDTRW5ITVJ?
SW780 MlrjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjKdXNoUUN3ME2wMlU4PTl3IN88US=> NGDzUIpUSU6JRWK=
MDA-MB-175-VII NGTIWIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoW5TWM2OD1yLkW3O|QyKM7:TR?= NV:1cpJUW0GQR1XS
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SW684 NEjNSHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfEbpRKSzVyPUGuPVc{PzFizszN MVLTRW5ITVJ?
NCI-H1693 M{TibWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfLUVNKSzVyPUGuPVgxQDZizszN Ml74V2FPT0WU
8-MG-BA NWi2W2ZRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnf0TWM2OD1zLkm4NlEh|ryP MUDTRW5ITVJ?
OVCAR-8 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDRTWM2OD1{LkCwNlk2KM7:TR?= MYrTRW5ITVJ?
DSH1 MnPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlvaTWM2OD1{LkCxO|c1KM7:TR?= NYHBNXFpW0GQR1XS
KYSE-520 NYD3c|lVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnQfHFzUUN3ME2yMlAzODR7IN88US=> NWn3OVZPW0GQR1XS
GB-1 NW\yOYo2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEPjSmRKSzVyPUKuNFI2PzdizszN NHm5TXdUSU6JRWK=
RCC10RGB MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTJwMES4O|Eh|ryP MmXhV2FPT0WU
SW954 NYLtU2R6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTJwMEWwN|Yh|ryP MVzTRW5ITVJ?
IST-MES1 M3yyUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTGXFFwUUN3ME2yMlA2PTZ6IN88US=> MYXTRW5ITVJ?
AU565 NIC1eHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjYR2NFUUN3ME2yMlA2QTB3IN88US=> NYP0ZldKW0GQR1XS
CW-2 M2fmWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVi1UFk2UUN3ME2yMlA3QDd3IN88US=> Mm\pV2FPT0WU
TE-1 NX7OTVBuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XYUGlEPTB;Mj6wPVM4PyEQvF2= MWHTRW5ITVJ?
KP-4 M{nKRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrITFBNUUN3ME2yMlExOzF{IN88US=> Ml\XV2FPT0WU
SNU-C2B M2nyRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTJwMUC4NlMh|ryP MVvTRW5ITVJ?
NCI-H1395 MmnWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3[4Z2lEPTB;Mj6xNFk5PiEQvF2= M12xdnNCVkeHUh?=
CAS-1 Ml;0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTJwMUG1OFYh|ryP NHLQTndUSU6JRWK=
NCI-H1155 NGPzPWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXK3O2lrUUN3ME2yMlIzPjlizszN NWriZYE2W0GQR1XS
SK-MEL-1 Mk[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTJwMkixPFEh|ryP NHO4UYpUSU6JRWK=
KINGS-1 NXLYOZMyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDEOpVzUUN3ME2yMlMxODh6IN88US=> NUnjN5JlW0GQR1XS
ESS-1 NVjmcplKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGm1VFlKSzVyPUKuN|A2OSEQvF2= MUPTRW5ITVJ?
PANC-10-05 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzqfnZ[UUN3ME2yMlM4ODJ2IN88US=> M4LXfXNCVkeHUh?=
HT-1376 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHFe29KSzVyPUKuN|g4ODhizszN NWHUWJY6W0GQR1XS
LS-513 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLPTWM2OD1{LkO4PFc1KM7:TR?= MWjTRW5ITVJ?
KNS-42 NIL2c2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH:0dlRKSzVyPUKuN|kyODVizszN NFuzSGhUSU6JRWK=
639-V NFzwXW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDsTWM2OD1{LkSyO|U5KM7:TR?= NF7qTXlUSU6JRWK=
SJRH30 NXjUUnoxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDNO3V6UUN3ME2yMlQ2OTZzIN88US=> MYrTRW5ITVJ?
SW1783 NEnNR4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTJwNEewO|gh|ryP NEPBXo9USU6JRWK=
NB6 M1vnfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTJwNEiyPEDPxE1? MomxV2FPT0WU
SK-MEL-30 NGLNTpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLDTWM2OD1{LkS4PFMh|ryP NF3YSZRUSU6JRWK=
HCE-4 NEfyd5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTJwNEm0NFIh|ryP M3HuNnNCVkeHUh?=
NB10 M3zrUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPPeohKSzVyPUKuOFk1OjlizszN NYThTJF{W0GQR1XS
H-EMC-SS M1\NUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTJwNUS2OUDPxE1? NXXrd5E1W0GQR1XS
SK-HEP-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTJwNUWyN|Yh|ryP M3LnPHNCVkeHUh?=
HCC1395 M37WW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfrUnB5UUN3ME2yMlU2PjV3IN88US=> MXLTRW5ITVJ?
HuCCT1 M3HUeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTJwNU[zPUDPxE1? NIXWUGtUSU6JRWK=
SiHa M2fIUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofjTWM2OD1{LkW4O|E6KM7:TR?= MWDTRW5ITVJ?
EFO-21 NED6NY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTJwNUi3OlQh|ryP NV;k[m9tW0GQR1XS
NCI-H1666 NILITlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDVTWM2OD1{LkW5NFc3KM7:TR?= NYDNO4NxW0GQR1XS
UMC-11 MnrTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTJwNUm4Olgh|ryP M{jtbHNCVkeHUh?=
COLO-679 NELjfm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDPW4RKSzVyPUKuOlEzOzNizszN M3HCVnNCVkeHUh?=
A388 NYi4SZhqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXBNXJiUUN3ME2yMlY2OjV|IN88US=> MYLTRW5ITVJ?
LB2518-MEL M4Ho[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\JU25MUUN3ME2yMlY2PDB6IN88US=> M1v2VnNCVkeHUh?=
EW-13 NVLZb5hJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTJwNki3Olgh|ryP MkjtV2FPT0WU
MLMA MlnXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofpTWM2OD1{Lk[4PFU3KM7:TR?= NEDS[5NUSU6JRWK=
HOP-92 NFrK[|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILqcHVKSzVyPUKuO|AyOSEQvF2= M4K1VHNCVkeHUh?=
NOMO-1 NYXNcW41T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3H1NGlEPTB;Mj63NVQ1OiEQvF2= NXTuWVVDW0GQR1XS
NCI-H810 MoXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLYTWM2OD1{LkeyNVk5KM7:TR?= Mn7CV2FPT0WU
769-P NFSwbWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;wSWlEPTB;Mj63NlI5KM7:TR?= NXnwSYdNW0GQR1XS
SAS MmK0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTJwN{[1JO69VQ>? Mo\5V2FPT0WU
M059J MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;MTWM2OD1{Lke4N|E5KM7:TR?= NXfvVphpW0GQR1XS
RD MkDBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvQNZZKSzVyPUKuO|k1PzFizszN NVfYPId{W0GQR1XS
GT3TKB M2nlZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DCeWlEPTB;Mj64N|Q{OyEQvF2= M1W1enNCVkeHUh?=
MZ7-mel M4nwTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{n3UGlEPTB;Mj64OlUxOiEQvF2= NHXjcWdUSU6JRWK=
KYSE-510 M2nWcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLndXZjUUN3ME2yMlg6PTB2IN88US=> MWPTRW5ITVJ?
NCI-N87 NWrIRo9CT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTJwOUG4NFch|ryP Ml3BV2FPT0WU
MKN1 MlHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HnUmlEPTB;Mz6wNFE3OyEQvF2= MWXTRW5ITVJ?
NCI-H2170 MlPIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXWenp1UUN3ME2zMlAxPDVzIN88US=> NI[zSXlUSU6JRWK=
OVCAR-3 M{LLNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XOSmlEPTB;Mz6wNlEyPSEQvF2= MmHSV2FPT0WU
KLE MoDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;NTWM2OD1|LkC2PFEzKM7:TR?= MUjTRW5ITVJ?
LB771-HNC MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrITWM2OD1|LkC5N|Q{KM7:TR?= MXXTRW5ITVJ?
MC116 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjuR21KSzVyPUOuNVEyOTJizszN M2m4eXNCVkeHUh?=
SCC-9 NIHORllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXtTWM2OD1|LkGxN|Y3KM7:TR?= M4LKUnNCVkeHUh?=
U031 NF[zZolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rF[GlEPTB;Mz6xNlQzOyEQvF2= M4jHdXNCVkeHUh?=
LCLC-103H MnPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlz5TWM2OD1|LkKzPFk1KM7:TR?= MkXVV2FPT0WU
U-266 MlnzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3:1Z2lEPTB;Mz6yO|Y{OyEQvF2= M1K4NXNCVkeHUh?=
JVM-2 M3n0fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvkb3hKSzVyPUOuN|IyPjRizszN NXTPVW91W0GQR1XS
UACC-257 M2XxcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHZVWlqUUN3ME2zMlQ3PDNzIN88US=> MkLjV2FPT0WU
LB996-RCC MonyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1frNGlEPTB;Mz60OlUxOiEQvF2= MkP5V2FPT0WU
BT-549 NHf4WYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrXbVFKSzVyPUOuOVAzODlizszN NHq1c3ZUSU6JRWK=
NCI-H526 NVn6bFh4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTHTWM2OD1|LkW0NVU5KM7:TR?= NVjTTolTW0GQR1XS
RH-18 NHPreWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTNwNkG3PVgh|ryP NYHub3BjW0GQR1XS
SW626 MlvrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XmXGlEPTB;Mz63NVEzQCEQvF2= NHzkVIhUSU6JRWK=
SK-N-AS Mn3sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XKW2lEPTB;Mz63N|I1PyEQvF2= NVr2VIU4W0GQR1XS
IGR-1 NY\yfJdmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLQboJKSzVyPUOuO|QzPDhizszN MoizV2FPT0WU
NCI-H2291 M2DveWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTIT2RKSzVyPUOuO|U{OzZizszN NInheVJUSU6JRWK=
NCI-H441 MnXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTNwOEK0N|Mh|ryP MnPtV2FPT0WU
D-247MG MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rQbGlEPTB;Mz64OlA{OSEQvF2= MYHTRW5ITVJ?
COLO-320-HSR MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\QT2k3UUN3ME2zMlkyOjdzIN88US=> M3HV[XNCVkeHUh?=
A253 Mk\0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PQfmlEPTB;Mz65NVQ{PiEQvF2= M1jWUnNCVkeHUh?=
HT55 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3T1[2lEPTB;Mz65NVc4OyEQvF2= MVnTRW5ITVJ?
MZ1-PC M4Xp[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjRTWM2OD1|Lkm1PFY1KM7:TR?= NGrr[oxUSU6JRWK=
RERF-LC-MS NGeyPFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrMSoVKSzVyPUOuPVY2OjVizszN MYnTRW5ITVJ?
SF268 M3Pa[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTPbnNjUUN3ME20MlEzQDhzIN88US=> NXW2UWw{W0GQR1XS
RMG-I NYi0Ro9bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTRwMkG0OVQh|ryP NHXSe2hUSU6JRWK=
CFPAC-1 NGLQdY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PqVmlEPTB;ND6yNVY5PSEQvF2= M4DY[HNCVkeHUh?=
S-117 NVHVflFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvTdZhKSzVyPUSuNlIxOjRizszN MUjTRW5ITVJ?
NCI-H2122 MlLyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zBWmlEPTB;ND6zNFk{OSEQvF2= MnnOV2FPT0WU
J82 M{ewRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnhTWM2OD12LkOzOFQyKM7:TR?= M1j4O3NCVkeHUh?=
CAL-72 NF;yco1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTOTWM2OD12LkO1OFc4KM7:TR?= Moe4V2FPT0WU
GP5d NF24NHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrOVVZKSzVyPUSuOFEzQThizszN MlHDV2FPT0WU
UACC-62 M1LGRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmS1TWM2OD12LkS2OVQ2KM7:TR?= NUjLPYNHW0GQR1XS
MS-1 M2jpSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HvZ2lEPTB;ND60PVEyPSEQvF2= M{S3XHNCVkeHUh?=
RCM-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nJNGlEPTB;ND61NlkzKM7:TR?= MUDTRW5ITVJ?
IST-SL1 NYjWRm1TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;zSYNGUUN3ME20MlY2OzV7IN88US=> MYjTRW5ITVJ?
SW756 NVLwN4VNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXjJR|UxRTRwNkmxN|Uh|ryP NUjRfINQW0GQR1XS
HCC38 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fXbmlEPTB;ND63NVE6PyEQvF2= M1\lUXNCVkeHUh?=
YAPC NW[1ZlNkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDEeHI1UUN3ME20MlcyOzF|IN88US=> M1XjR3NCVkeHUh?=
DK-MG MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvEWoNKSzVyPUSuO|gyOTJizszN MVPTRW5ITVJ?
HCC1937 NUDud2Y1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\tfI5KSzVyPUSuPFUyOjVizszN MY\TRW5ITVJ?
OAW-28 NGLhSpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTafnZWUUN3ME20MlkzPDN3IN88US=> Mn74V2FPT0WU
MEG-01 MlPKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTVwMEizNlgh|ryP Ml3VV2FPT0WU
GI-1 NUTF[nlFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYH2[FhZUUN3ME21MlI{PDh4IN88US=> MUHTRW5ITVJ?
FADU M2nsfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXie|lKSzVyPUWuNlc5OzdizszN NYPH[m1VW0GQR1XS
CAPAN-1 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\QTWM2OD13LkO5OFMzKM7:TR?= NWroZ4dnW0GQR1XS
TGBC11TKB MnnVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYC2fphqUUN3ME21MlQ2Pzh4IN88US=> Mm\HV2FPT0WU
DBTRG-05MG NUPqTIU6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTVwNUC1NkDPxE1? NFzlfVdUSU6JRWK=
ONS-76 NXTVfHBUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nIe2lEPTB;NT61OVgxOSEQvF2= Mk\IV2FPT0WU
Ca-Ski MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3TTUWlEPTB;NT62NlU3PiEQvF2= NFfMUpJUSU6JRWK=
COLO-741 NUj0[ZVFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlT1TWM2OD13LkexPVk2KM7:TR?= MU\TRW5ITVJ?
HT-29 Ml6zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkT1TWM2OD13LkiwOFc2KM7:TR?= NFHKfIVUSU6JRWK=
HCC1187 NWPVZnVET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTVwOEGwNFgh|ryP M33BeHNCVkeHUh?=
Capan-2 NVLKRpR5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjKe2M6UUN3ME21Mlk2QTl2IN88US=> MlzxV2FPT0WU
NCI-H630 NVyyTmsxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[xeIpKSzVyPU[uNVE1PDFizszN MYHTRW5ITVJ?
COLO-678 M2TyOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXuTWM2OD14LkG1NFkzKM7:TR?= MkHuV2FPT0WU
D-502MG NWf3PXNyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\VSmlEPTB;Nj6xO|M{PiEQvF2= MlzsV2FPT0WU
NB17 NHO3[olIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELM[WJKSzVyPU[uNlQ6PDNizszN NFHhemZUSU6JRWK=
SK-MEL-24 NYriRZJOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTZwMk[0PUDPxE1? MWDTRW5ITVJ?
NCI-SNU-5 M1jCNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXZTWM2OD14LkK3NVE5KM7:TR?= MYnTRW5ITVJ?
D-392MG MnXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrHSmxTUUN3ME22MlM{Ozl7IN88US=> M4PN[3NCVkeHUh?=
A498 M2f0TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTZwNECzNFIh|ryP Mn3kV2FPT0WU
OCI-AML2 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nmZmlEPTB;Nj60OVQ1PSEQvF2= MVrTRW5ITVJ?
HCC2157 NHXTdJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEPNU4hKSzVyPU[uOFU3OTZizszN M4XyRnNCVkeHUh?=
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COG-LL-317 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILYfZJKSzVyPUGuN{BvVQ>? MXWyNVM{PzZ5OR?=
RS4;11 MmnlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVHUfXd[UUN3ME2zOUBvVQ>? M3ntelIyOzN5Nke5
MOLT-4 NY[2Unh{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfyXWd4UUN3ME24MlYhdk1? NH60ZY0zOTN|N{[3PS=>
CCRF-CEM MonYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTRwMTDuUS=> M4f4NlIyOzN5Nke5
Kasumi-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTFwNDDuUS=> MnfQNlE{Ozd4N{m=
Karpas-299 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVX6[mpHUUN3ME2yNU44KG6P M3nsVFIyOzN5Nke5
Ramos-RA1 MnTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRThwMjDuUS=> NWPuVnR[OjF|M{e2O|k>
H838 NXnpU5RQTnWwY4Tpc44hSXO|YYm= Mo\FNUDPxE1? NWWwdJhiPDhiaB?= MV;pcoR2[2W|IHPlcIwh[XW2b4DoZYd6 NGCyfokzODN4NEGxNy=>
H838 NXjkVYd[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\TfFIxOC1|IN88US=> NGjZNHg4OiCq NYr2SXBIcW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 M1\1VlIxODJ6OEW0
A549  NYrwdZltT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLJRlgzOC1|IN88US=> M{fnflczKGh? Mm\abY5lfWOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh[XW2b4DoZYd6 NUX6c5lnOjByMki4OVQ>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; 

PubMed: 24457069     


Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙

p-EGFR / EGFR; 

PubMed: 25654224     


PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot;

p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; 

PubMed: 25654224     


PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.

24457069 25654224
Immunofluorescence
p-4EBP1 / EIF4E / mTOR; 

PubMed: 24600487     


(C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋€𢡄�෋€䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemセ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒セ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wセ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v 玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴￿￿嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗

LC3B / p62 ; 

PubMed: 30337536     


Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞

24600487 30337536
Growth inhibition assay
Cell viability ; 

PubMed: 29263324     


Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.

29263324
In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID