AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 38 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

  • HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

    Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017, 15(1):9. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 M4jmWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmH0TWM2OD1yLkCyN|MyKM7:TR?= NE\NdolUSU6JRWK=
NCI-H720 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHITWM2OD1yLkCyPVM5KM7:TR?= M1raV3NCVkeHUh?=
KARPAS-45 NFfDc5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTBwMEOzO|Eh|ryP NGHLcXBUSU6JRWK=
EW-3 MoC1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjJcYlKSzVyPUCuNFQxPTZizszN M3\GZXNCVkeHUh?=
SBC-1 M{jvOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXq5XnUxUUN3ME2wMlA1PThzIN88US=> MnfrV2FPT0WU
NCI-H209 NFe0U|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml:3TWM2OD1yLkC1OFU{KM7:TR?= M{X1NHNCVkeHUh?=
EoL-1-cell MorqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmS4TWM2OD1yLkC2OVY6KM7:TR?= MX;TRW5ITVJ?
H9 NEnK[ZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1:0fWlEPTB;MD6wOlY6OiEQvF2= NIrZfpBUSU6JRWK=
AN3-CA NHPiWZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY[3eG1jUUN3ME2wMlA4QTVzIN88US=> M4K3R3NCVkeHUh?=
HH NGnoWHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrjTWM2OD1yLkC4NFA6KM7:TR?= MXrTRW5ITVJ?
KU812 NV;wbIdHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLiTWM2OD1yLkC4NFQ{KM7:TR?= MoO3V2FPT0WU
DU-4475 NXj0TVNHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGO3NJZKSzVyPUCuNFgxPDdizszN NInob3FUSU6JRWK=
CTV-1 NHfReJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTBwMEi2OFEh|ryP NFTt[ZBUSU6JRWK=
BV-173 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LsVmlEPTB;MD6wPVA3OSEQvF2= NYDaflZxW0GQR1XS
ES5 M1[yS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HTRmlEPTB;MD6wPVM2KM7:TR?= NUHxVXkyW0GQR1XS
G-402 NWLJT|ZIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TaTGlEPTB;MD6wPVk5KM7:TR?= NVu0OGRqW0GQR1XS
MV-4-11 NXXTWWo5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjQO|RKSzVyPUCuNVA{PDdizszN MVfTRW5ITVJ?
NB69 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PVSGlEPTB;MD6xN|Uh|ryP MYHTRW5ITVJ?
EGI-1 M2nUT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTwR4JKSzVyPUCuNVM2OzFizszN NGjyOWdUSU6JRWK=
EW-18 M1LmZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nhN2lEPTB;MD6xOFA4OyEQvF2= MXzTRW5ITVJ?
ME-180 M4L4Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13jfWlEPTB;MD6xOFY5OSEQvF2= MVjTRW5ITVJ?
D-283MED NEXoNm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7ETWM2OD1yLkG0O|I{KM7:TR?= NUHYV3FzW0GQR1XS
NB14 NUK4eVJ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrRTWM2OD1yLkG2OFQ4KM7:TR?= MnezV2FPT0WU
Ramos-2G6-4C10 NWfnTJhwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPGTWM2OD1yLkG2PVg{KM7:TR?= NFXxeW1USU6JRWK=
NBsusSR Mn7SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTBwMUewNVkh|ryP M13renNCVkeHUh?=
LAMA-84 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nVTGlEPTB;MD6xPFA{OiEQvF2= MmT2V2FPT0WU
NCI-H1581 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTBwMUi5OlYh|ryP NVflZXozW0GQR1XS
A204 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFP4bmdKSzVyPUCuNVg6PyEQvF2= NHzsdXhUSU6JRWK=
A549 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXL1VmJUUUN3ME2wMlE6PTVizszN NILGUWNUSU6JRWK=
CAL-51 NYT5VVAzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTBwMUm2OVEh|ryP NXOycXhoW0GQR1XS
NKM-1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3oTWM2OD1yLkKwNFI6KM7:TR?= MXLTRW5ITVJ?
LoVo MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWftZ|JNUUN3ME2wMlIxOjB{IN88US=> NVTWTXJKW0GQR1XS
HAL-01 M17KTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrKcpFKSzVyPUCuNlAzQTdizszN NGLRbHlUSU6JRWK=
HCC2218 NXT3UGxxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTBwMkC2PFgh|ryP MXPTRW5ITVJ?
BEN M3r5[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkP6TWM2OD1yLkKxNlk{KM7:TR?= MXPTRW5ITVJ?
LC-2-ad NGfRNXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\VZZBKSzVyPUCuNlIxQDRizszN Ml7MV2FPT0WU
LXF-289 NXL6SVJxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVP1[FJsUUN3ME2wMlI2ODRzIN88US=> MoH4V2FPT0WU
HGC-27 M4jWeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\iTWM2OD1yLkK2OFE1KM7:TR?= MXXTRW5ITVJ?
LK-2 NIjIcoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DDW2lEPTB;MD6yOlgzPCEQvF2= MmTzV2FPT0WU
CTB-1 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rMV2lEPTB;MD6yO|IyPSEQvF2= NV3FflI1W0GQR1XS
697 M1iwT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1n5[mlEPTB;MD6yO|M3PyEQvF2= M3XrWXNCVkeHUh?=
ES7 NUnOe28{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXOR45KSzVyPUCuNlc{QDNizszN NGPoRnhUSU6JRWK=
MG-63 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LGXGlEPTB;MD6yO|QxPyEQvF2= M{jGZnNCVkeHUh?=
T47D MoHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\4UlNKSzVyPUCuNlc1PzVizszN M4fESXNCVkeHUh?=
RPMI-8226 NU\Id5J{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk[4TWM2OD1yLkK4OVg6KM7:TR?= NHXVWmVUSU6JRWK=
NCI-H292 M3m4[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nEdmlEPTB;MD6yPVE3PCEQvF2= NYDFSXhvW0GQR1XS
HT MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTBwMkmzPEDPxE1? MkKwV2FPT0WU
A3-KAW NGHocpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTBwMkm3OEDPxE1? Mmq3V2FPT0WU
LB2241-RCC NXvzenJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTBwM{CxPVgh|ryP NIjYRZJUSU6JRWK=
BB65-RCC MoLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;SNHJHUUN3ME2wMlMxQDB3IN88US=> MnnjV2FPT0WU
G-401 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfWSI9KSzVyPUCuN|A6QDZizszN M{TMTXNCVkeHUh?=
TYK-nu M3z6Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTBwM{G4OlQh|ryP M135bHNCVkeHUh?=
NCI-SNU-1 NXHTSpNST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTBwM{KzN|Eh|ryP MoOyV2FPT0WU
CHL-1 NInafYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHlVYtKSzVyPUCuN|I4ODNizszN M1fFUXNCVkeHUh?=
P30-OHK MnfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTMWHFxUUN3ME2wMlMzPzB3IN88US=> M1jyTnNCVkeHUh?=
PA-1 M3;YOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorGTWM2OD1yLkOzOlE6KM7:TR?= MlP1V2FPT0WU
LB1047-RCC M{KxUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTBwM{SwOFch|ryP NWP2[HJDW0GQR1XS
EM-2 NID1XIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYH1W4FoUUN3ME2wMlM1OTh6IN88US=> NYjvZ5J5W0GQR1XS
VA-ES-BJ Mlv5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jIcmlEPTB;MD6zOFI3QCEQvF2= M{nvcnNCVkeHUh?=
NCI-H520 NVrYSXU3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2jEVWlEPTB;MD6zOFg{PCEQvF2= MnTJV2FPT0WU
ECC10 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTBwM{WwN|Uh|ryP NXruVlVsW0GQR1XS
LNCaP-Clone-FGC NELLW4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfoTWM2OD1yLkO1N|E1KM7:TR?= MWHTRW5ITVJ?
ES8 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETqXIdKSzVyPUCuN|Y2PTNizszN M4PhRXNCVkeHUh?=
HuO-3N1 MmnwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDUb3lMUUN3ME2wMlM5Ozd7IN88US=> MnnnV2FPT0WU
L-363 NVzqPYtIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTBwM{mwNlYh|ryP Mm\oV2FPT0WU
SK-LU-1 MnHlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnx[HNZUUN3ME2wMlM6OjB6IN88US=> M2D6ZXNCVkeHUh?=
MCF7 NHzuOVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIL4eGpKSzVyPUCuOFA2OyEQvF2= MVPTRW5ITVJ?
HuO9 M1jlbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGe0VFhKSzVyPUCuOFA3PjdizszN NGfIU|NUSU6JRWK=
BHT-101 NVjuXHN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTBwNEC3PFch|ryP NXXQNXBIW0GQR1XS
MZ2-MEL M3rOTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3[yOmlEPTB;MD60NFg5OiEQvF2= MofpV2FPT0WU
NOS-1 NVTvU3d5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnmWVZRUUN3ME2wMlQyODd2IN88US=> M1G3NXNCVkeHUh?=
NEC8 Mke1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;wNWlEPTB;MD60NVY6PSEQvF2= NHjYWXRUSU6JRWK=
COR-L88 NYfnWpd[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LrUmlEPTB;MD60NlI3PyEQvF2= M2rySXNCVkeHUh?=
MFM-223 NX;LOFZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTBwNEK2NVch|ryP M2nLeXNCVkeHUh?=
HSC-3 Mlv5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGP0PXZKSzVyPUCuOFI6ODhizszN NIm2e3JUSU6JRWK=
OVCAR-5 M4HJVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4fReGlEPTB;MD60Nlk{OyEQvF2= NGXWOWZUSU6JRWK=
MFE-280 NHXMW4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHYTWM2OD1yLkSzNFMzKM7:TR?= MnPkV2FPT0WU
KP-N-YS NGL4dppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jTPGlEPTB;MD60N|A4KM7:TR?= MVvTRW5ITVJ?
EPLC-272H MlnvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm[1TWM2OD1yLkSzNFc4KM7:TR?= M3PJN3NCVkeHUh?=
KE-37 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzXTWM2OD1yLkSzNlQ4KM7:TR?= MnfrV2FPT0WU
HuH-7 M3jZPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LlTmlEPTB;MD60N|M4OiEQvF2= NUCwUGFzW0GQR1XS
CHP-212 NFzTdGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzwTFNKSzVyPUCuOFM3QTdizszN NUjocWZNW0GQR1XS
SK-MES-1 MmDmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTBwNEO4OFgh|ryP NFLESotUSU6JRWK=
SJSA-1 MlzoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnDdFlKSzVyPUCuOFQ2PTRizszN NF7zSZFUSU6JRWK=
QIMR-WIL M4O5Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnIblFUUUN3ME2wMlQ2OTVizszN MVfTRW5ITVJ?
ES4 NF;MWYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXv3dXhGUUN3ME2wMlQ2OzN4IN88US=> MVzTRW5ITVJ?
TI-73 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnWTWM2OD1yLkS1Olg3KM7:TR?= NH7WcZBUSU6JRWK=
COLO-684 M2e2c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPIe4ZFUUN3ME2wMlQ2PzJ4IN88US=> M3fMUHNCVkeHUh?=
GCIY NHTlWZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHiToJKSzVyPUCuOFU4PTRizszN NX\BOVd7W0GQR1XS
JVM-3 MlrtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzH[4czUUN3ME2wMlQ3Ojl{IN88US=> M2ri[XNCVkeHUh?=
MPP-89 MnfqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnz0TWM2OD1yLkS2PVQ4KM7:TR?= NFn0XIJUSU6JRWK=
M14 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXVOZk6UUN3ME2wMlQ4OTdizszN MkjaV2FPT0WU
SW962 NIK3WHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTBwNEexPUDPxE1? MmnJV2FPT0WU
VM-CUB-1 NYnib4ZYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1u5VmlEPTB;MD60O|I5PiEQvF2= MUnTRW5ITVJ?
A101D NGHaeHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7aSVZKSzVyPUCuOFg6PjhizszN MV3TRW5ITVJ?
DB NV;Ofmt[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTBwNUCwNlEh|ryP M3zGdnNCVkeHUh?=
KNS-62 MmrkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3sco5rUUN3ME2wMlUxPTZ6IN88US=> NF;vRVhUSU6JRWK=
NCI-H460 M4e3eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7uUYxYUUN3ME2wMlUxPTl5IN88US=> MXnTRW5ITVJ?
MDA-MB-361 MlvvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7qTWM2OD1yLkWwPFch|ryP NWP4XG9SW0GQR1XS
DOK Mm\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nTUGlEPTB;MD61NVM{OyEQvF2= MWXTRW5ITVJ?
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Rh41 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXEfoszUUN3ME2zNE44KG6P MlfMNlE{Ozd4N{m=
Rh18 NVLyUGlnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn23TWM2OD12MT60JI5O NXvBVoJ[OjF|M{e2O|k>
Rh30 MkLtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHLsWJBKSzVyPUG0JI5O MY[yNVM{PzZ5OR?=
BT-12 NHf1UHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXqTWM2OD1{ND63JI5O MXWyNVM{PzZ5OR?=
CHLA-266 MnHzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHLreZpKSzVyPUGuNUBvVQ>? Mnq2NlE{Ozd4N{m=
TC-71 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTR4LkWgcm0> MUKyNVM{PzZ5OR?=
CHLA-9 NFz0RoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHSTWM2OD1zNT6zJI5O NYj2d2NIOjF|M{e2O|k>
CHLA-10 NFG0TZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\MbnJKSzVyPUOuPUBvVQ>? NInvRVEzOTN|N{[3PS=>
CHLA-258 NYjzSI9XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTFyLkSgcm0> M2TTZlIyOzN5Nke5
GBM2 NGjHXndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUS3NYF{UUN3ME20NE44KG6P MVSyNVM{PzZ5OR?=
NB-1643 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTN|Lkigcm0> NGPqToszOTN|N{[3PS=>
NB-EBc1 MnH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHBWmNKSzVyPUK3MlEhdk1? NXrVd5BkOjF|M{e2O|k>
CHLA-90 MlfzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTN2LkWgcm0> M{PKVFIyOzN5Nke5
CHLA-136 M{K0dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\yeoVKSzVyPUG2Nk43KG6P NEXxNG4zOTN|N{[3PS=>
NALM-6 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTN{Lkmgcm0> MUKyNVM{PzZ5OR?=
COG-LL-317 MoDyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTFwMzDuUS=> NUW3NFdHOjF|M{e2O|k>
RS4;11 MnLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1K5RmlEPTB;M{Wgcm0> M{jGTVIyOzN5Nke5
MOLT-4 NVvnTW9iT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\1TWM2OD16Lk[gcm0> NF65[JQzOTN|N{[3PS=>
CCRF-CEM NYP4b|NKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITnNpNKSzVyPUSuNUBvVQ>? NIGzRXIzOTN|N{[3PS=>
Kasumi-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLhTWM2OD1zLkSgcm0> M2S5bVIyOzN5Nke5
Karpas-299 MlXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnH2TWM2OD1{MT63JI5O M{HCZ|IyOzN5Nke5
Ramos-RA1 NU\JN2J{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRThwMjDuUS=> NX7K[XZkOjF|M{e2O|k>
H838 NUHrbXhGTnWwY4Tpc44hSXO|YYm= MlexNUDPxE1? NHy5clQ1QCCq NWHROHl2cW6mdXPld{Bk\WyuIHH1eI9xcGGpeR?= NVK0SoxCOjB|NkSxNVM>
H838 NWjsc3F6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2X3TVAuOyEQvF2= MVm3NkBp MnXIbY5lfWOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh[XW2b4DoZYd6 MmLuNlAxOjh6NUS=
A549  NXHXUopWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHz2V3MxNTNizszN NGjYfFg4OiCq MXTpcoR2[2W|IHfyc5d1cCCrbnjpZol1cW:wIHHu[EBifXSxcHjh[5k> MYCyNFAzQDh3NB?=

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca null Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID