Doxycycline

For research use only.

Catalog No.S5159 Synonyms: Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum

2 publications

Doxycycline Chemical Structure

Molecular Weight(MW): 444.43

Doxycycline is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa.

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Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Doxycycline is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa.
In vitro

100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used[1]. Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 MULDfZRwfG:6aXPpeJkh[XO|YYm= MmDLO|IhcA>? MlHmR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bi\nSncjC3NkBpenNiYomg[oxwfyCleYTvcYV1enluIFnDOVA:OTVizszN MnvaNVk1QDJ2N{[=
HepG2 M3fKTWN6fG:2b4jpZ4l1gSCjc4PhfS=> NGfuVXA4OiCq MkLUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2yNEDPxE1? NYjTeGpKOTl2OEK0O|Y>
THP1 NH\pToJEgXSxdH;4bYNqfHliYYPzZZk> NYjuN3ljPzJiaB?= NFTGWG1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVUFBzIHPlcIx{KGGodHXyJFczKGi{czDifUBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nMYJie2WmIH\sc5ch[3m2b33leJJ6NCCLQ{WwQVIxKM7ebR?= MUKxPVc1QDd6MR?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
AKT / BCL6 / Cyclin E / HDAC2 / HDAC3 / NEMO / TYK2 / RIPK1 ; 

PubMed: 26142707     


Doxycycline treatment decreases the levels of HSP90 client proteins in DLBCL cells. The DLBCL cells were treated with the indicated concentrations of doxycycline for 24 hrs. The levels of HSP90 client proteins in these cells were analyzed by western blotting.

HSP70 / HSP90 ; 

PubMed: 26142707     


Reduced protein levels of HSP70 and HSP90 in DLBCL cells treated with doxycycline. DLBCL cells were treated with doxycycline for 24 hrs, and the levels of HSP70 and HSP90 were analyzed by western blotting. 

pATM / ATM ; 

PubMed: 23282955     


Levels of phosphorylated ATM and overall ATM was determined in the indicated cell lines at the indicated times after treatment with 1μg/ml doxycycline, as detected by Western Blotting.

26142707 23282955
Immunofluorescence
E-cadherin / Vimentin ; 

PubMed: 29285218     


Doxycycline increases E-cadherin expression and decreases Vimentin expression, whereas thrombin partly counteracts this inhibitory effect in MCF-7 cells.

29285218
In vivo Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)[2].

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines
  • Concentrations: 100 ng/mL, 1 µg/mL
  • Incubation Time: 24, 72, and 96 hours
  • Method:

    Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 88 mg/mL (198.0 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 444.43
Formula

C22H24N2O8

CAS No. 564-25-0
Storage powder
in solvent
Synonyms Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum
Smiles CC1C2C(O)C3C(N(C)C)C(=C(C(N)=O)C(=O)C3(O)C(=C2C(=O)C4=C1C=CC=C4O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04269434 Not yet recruiting Other: No Screening Neisseria Gonorrhoeae Infection|Chlamydia Trachomatis Infection Institute of Tropical Medicine Belgium May 2020 Not Applicable
NCT03709459 Recruiting Drug: Doxycycline STIs Prevention Kirby Institute|South Australian Health and Medical Research Institute|Monash University December 17 2019 --
NCT03558984 Not yet recruiting Drug: D-PLEX|Other: Standard of Care Surgical Site Infection|Sternal Infection|Cardiac Surgery PolyPid Ltd. October 2019 Phase 3
NCT02864550 Not yet recruiting Drug: Doxycycline|Other: Placebo Syphilis|Sexually Transmitted Infections British Columbia Centre for Disease Control August 15 2019 Phase 4
NCT03633123 Active not recruiting Drug: D_PLEX|Other: Standard of Care (SoC) Abdominal Surgery|Colon Surgery|Post-Op Infection PolyPid Ltd. October 4 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID