Doxycycline

For research use only.

Catalog No.S5159 Synonyms: Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum

12 publications

Doxycycline Chemical Structure

CAS No. 564-25-0

Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.

Selleck's Doxycycline has been cited by 12 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.
In vitro

100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used[1]. Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Staphylococcus aureus 2 planktonic cells NVnkbZROSmGldHXybYNq\GGuIHHzd4F6 NVzTTVdVOjRiaILz MWnCZYN1\XKrY3nkZYwh[WO2aY\peJkh[WejaX7zeEBu\XSqaXPpcIxqdi2{ZYPpd5RidnRiU4ThdIh6dG:lb3PjeZMh[XW{ZYXzJFIheGyjbnv0c45q[yClZXzsd{Bi\nSncjCyOEBpenNiYomgZ4Ft\2G{eTDibY9ncWyvIHTleolk\SCvZYToc4QtKE2EQ{2y{txONg>? Mlf1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4M{ixNlEoRjJ7NkO4NVIyRC:jPh?=
THP1 MmXzSpVv[3Srb36gZZN{[Xl? MYLT[Yxm[3Srdnn0fUBqdmSneDygdoF1cW9ib3[gTWM2OCCob4KgbJVu[W5iVFjQNUBk\WyuczD0c{BKSzVyIH\vdkBRdGG|bX;kbZVuKG[jbHPpdIFzfW1uIFnDOVA:Oy5zzszNMi=> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd2OEe4NUc,OTl5NEi3PFE9N2F-
K562 MnzoR5l1d3SxeHnjbZR6KGG|c3H5 M{D6blczKGi{cx?= MYLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLOVYzKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDmcI94KGO7dH;t[ZRzgSxiSVO1NF0yPc7:TT6= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6MkS3Okc,OTl2OEK0O|Y9N2F-
K562 NUn5eY16S3m2b4TvfIlkcXS7IHHzd4F6 NE\zRVQ4OiCqcoO= NGLzWoNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGGodHXyJFczKGi{czDifUBndG:5IHP5eI9u\XS{eTygTWM2OD1zNd88UU4> MnXRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Mk[xO|MoRjF7OUK2NVc{RC:jPh?=
HepG2 M13VOGN6fG:2b4jpZ4l1gSCjc4PhfS=> NX62XW5RPzJiaILz MXXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUKw{txONg>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6MkS3Okc,OTl2OEK0O|Y9N2F-
HepG2 MWnDfZRwfG:6aXPpeJkh[XO|YYm= M{DqVVczKGi{cx?= Mn6zR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2yNO69VS5? NFPHO4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmyOlE4Oyd-MUm5NlYyPzN:L3G+
THP1 NGXWbFNEgXSxdH;4bYNqfHliYYPzZZk> NWfvT3ptPzJiaILz M{\IWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHRJWDFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnetZoF{\WRiZnzve{BkgXSxbXX0dpktKEmFNUC9NlDPxE1w M1TCVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{S4O|gyLz5zOUe0PFc5OTxxYU6=
HepG2 Mn\3R5l1d3SxeHnjbZR6KGG|c3H5 NIm2[JQ4OiCqcoO= NITS[2hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCFQ{WwQVIx|ryPLh?= NIG4fYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe0NVE{OSd-MkG3OFEyOzF:L3G+
HepG2 MV7DfZRwfG:6aXPpeJkh[XO|YYm= M1XrNFczKGi{cx?= MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBESzVyPUKw{txONg>? NWXuVWhPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4PFk2PTlpPkKyPFg6PTV7PD;hQi=>
HepG2 NGrTV4xEgXSxdH;4bYNqfHliYYPzZZk> NF\4[JM4OiCqcoO= NHnkN2hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCFQ{WwQVIx|ryPLh?= M1;5NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEWyNVMzLz5{MUi1NlE{OjxxYU6=
HepG2 NHPyNG9EgXSxdH;4bYNqfHliYYPzZZk> MU[3NkBpenN? MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBESzVyPUKw{txONg>? M{HIb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUS2NlE3Lz5{NEm0OlIyPjxxYU6=
HepG2 MoLjR5l1d3SxeHnjbZR6KGG|c3H5 MmDUO|IhcHK| NYe2dFZIS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiQ1O1NF0zOM7:TT6= MkL2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5OUG2O|UoRjJ3N{mxOlc2RC:jPh?=
HepG2 M2\mfmN6fG:2b4jpZ4l1gSCjc4PhfS=> NF;DfoE4OiCqcoO= M2DaSmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGmwY4XiZZRm\CCob4KgO|IhcHK|IHL5JG1VXCCjc4PhfUwhS0N3ME2yNO69VS5? NWDHc5NyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyPFIzPjdpPkK1NlgzOjZ5PD;hQi=>
HepG2 MlvHR5l1d3SxeHnjbZR6KGG|c3H5 NHnXXGs4OiCqcoO= M{K2SmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KG2nYYP1doVlKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEOFNUC9NlDPxE1w MonFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNUW0OlMoRjJ5MUW1OFY{RC:jPh?=
HepG2 MX\DfZRwfG:6aXPpeJkh[XO|YYm= NFrXUmw4OiCqcoO= NFX6UmZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygR2M2OD1{MN88UU4> NXG2XHZJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OVQ{QTVpPkK3OlU1Ozl3PD;hQi=>
SW1353 NWi2Nm9WTnWwY4Tpc44h[XO|YYm= NHO2bZc2OCC3TR?= MWDJcohq[mm2aX;uJI9nKEmOLUGtZoV1[S2rbnT1Z4VlKE2PUEGzJJBzd2S3Y4Tpc44hcW5iaIXtZY4hW1dzM{WzJINmdGy|IHH0JFUxKHWP NHi5c3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K2O|IzPyd-MUeyOlczOjd:L3G+
K-12 BW25113 M{TQfWJi[3SncnnjbYRidCCjc4PhfS=> MV[yOEBpenN? MW\CZYN1\XKrY3nkZYwh[WO2aY\peJkh[WejaX7zeEB6[W[TIHflcoUu\GWoaXPp[Y51KEW|Y3jldolkcGmjIHPvcIkhUy1zMjDCW|I2OTF|IHLpc4ZqdG1iYYPz[ZN{\WRiYYOgcI9oKHKnZIXjeIlwdiCxZjD2bYFjdGViY3XscJMh[W[2ZYKgNlQhcHK| MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTNyN{O3OUc,OTl|MEezO|U9N2F-
K-12 BW25113 MWnCZYN1\XKrY3nkZYwh[XO|YYm= M1vwXVI1KGi{cx?= MXrCZYN1\XKrY3nkZYwh[WO2aY\peJkh[WejaX7zeEBGe2OqZYLpZ4hq[SClb3zpJGsuOTJiQmeyOVEyOyCkaX;mbYxuKGijcnLvdolv\yCyQ1GyOG4heHSjY{q6fYFnWSCybHHzcYllKGG|c3Xzd4VlKGG|IHzv[{Bz\WS3Y4Tpc44hd2ZidnnhZoxmKGOnbHzzJIFnfGW{IEK0JIhzeyCycnX0doVifGWmIIfpeIghPSC3TTDv[kBKWFSJIH\vdkA1KGi{cx?= NXj4TVduRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzNFc{PzVpPkG5N|A4Ozd3PD;hQi=>
Neuro2a MWfGeY5kfGmxbjDhd5NigQ>? NGPTNWYyKHWP NEXGPHNF\WO{ZXHz[UBqdiB5LVTIR{Bt\X[nbIOgbY4hTGilcket[IVncWOrZX70JI1wfXOnIF7leZJwOmFiY3XscJMh[XRiMTD1UUBjgSCOQz3NV{9ISy2PUzDhcoFtgXOrcx?= NV[xTJBORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3PFk3PTdpPkK2O|g6PjV5PD;hQi=>
vascular endothelial cells M{\zW2FvfGmkYXP0[ZJq[WxiYYPzZZk> M3zWU|I2KHWpL33s M3;iblczKGi{cx?= MWLBcpRq[mGldHXybYFtKGGldHn2bZR6KGGpYXnud5QhWmmla3X0eJNq[SCycn;3ZZpmc2mrIIP0dk4hSnKnaX7sJIlv\mWldHXkJIlvKEOGIILheEBxemmvYYL5JJB2dG2xbnHyfUB3[XOldXzhdkBmdmSxdHjlcIlidCClZXzsd{Bie3Onc4Pl[EBieyCkYXP0[ZJq[Wxic3jhdIUh[2ijbnflJIF1KDJ3IIXnM41tKG2nYYP1doVlKDd{IHjyd{Bxd3O2IHnu[oVkfGmxbjDifUBJd2WlaIP0JFM{OjV6L4DoZYxtd2mmaX6gd5RicW6rbnegZoF{\WRiZnz1c5I> NEHYV3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PVM2OCd-MkiwPFk{PTB:L3G+
A673 NX\UdVZKeUiWUzDhd5NigQ>? MkfndWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEF4N{OgZ4VtdHN? Mk\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-12 M3n1cpFJXFNiYYPzZZk> MlPTdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUGyJINmdGy| M2nxOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Hep2 NUfFVo5jSW62aT3DbIxidXmmaXHsJIF{e2G7 MYOxJJVoN22u M4f2RlghcHK| NH3MWY9CdnSrLVPocIFugWSrYXygZYN1cX[rdImgZYdicW6|dDDDbIxidXmmaXGgeJJi[2ixbXH0bZMhW2W{b4\hdkBNT1ZvTEKgbY5n\WO2ZXSgbY4hUGWyMjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4he2m8ZTDhcoQhdnWvYnXyJI9nKGOqbHHtfYRq[WxiaX7jcJV{cW:wIHH0JFEhfWdxbXygeJJm[XSnZDDheEA5KGi{czDwc5N1NWmwZnXjeIlwdiCjbnSgNlQhcHK|IHzheIVzKHKnLXnu[oVkfGmwZzDmdoV{cCCKZYCyJINmdGy|IH3vco9t[Xl? M36wTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzN{MkK3PVQ5Lz5|MkKyO|k1QDxxYU6=
skeletal myoblast cells MXLDfZRwfG:6aXPpeJkh[XO|YYm= M1PYbWRPTEl8IFP5eI91d3irY3n0fUBqdiCYaYTyc{whPzJiaH;1dkwhcW5icnH0JJNs\WyndHHsJI16d2KuYYP0JINmdGy|LDDJR|UxRTF2LkS5{txONg>? M4SxclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1OzNxJ{7DbGVOSkx:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
AKT / BCL6 / Cyclin E / HDAC2 / HDAC3 / NEMO / TYK2 / RIPK1 ; 

PubMed: 26142707     


Doxycycline treatment decreases the levels of HSP90 client proteins in DLBCL cells. The DLBCL cells were treated with the indicated concentrations of doxycycline for 24 hrs. The levels of HSP90 client proteins in these cells were analyzed by western blotting.

HSP70 / HSP90 ; 

PubMed: 26142707     


Reduced protein levels of HSP70 and HSP90 in DLBCL cells treated with doxycycline. DLBCL cells were treated with doxycycline for 24 hrs, and the levels of HSP70 and HSP90 were analyzed by western blotting. 

pATM / ATM ; 

PubMed: 23282955     


Levels of phosphorylated ATM and overall ATM was determined in the indicated cell lines at the indicated times after treatment with 1μg/ml doxycycline, as detected by Western Blotting.

26142707 23282955
Immunofluorescence
E-cadherin / Vimentin ; 

PubMed: 29285218     


Doxycycline increases E-cadherin expression and decreases Vimentin expression, whereas thrombin partly counteracts this inhibitory effect in MCF-7 cells.

29285218
In vivo Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)[2].

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines
  • Concentrations: 100 ng/mL, 1 µg/mL
  • Incubation Time: 24, 72, and 96 hours
  • Method:

    Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 88 mg/mL (198.0 mM)
Ethanol '-1 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 444.43
Formula

C22H24N2O8

CAS No. 564-25-0
Storage powder
in solvent
Synonyms Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum
Smiles CC1C2C(C3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04838717 Not yet recruiting Drug: BPG|Drug: Doxycycline Syphilis Assistance Publique - Hôpitaux de Paris May 2021 Phase 3
NCT04860505 Recruiting Drug: Doxycycline|Drug: Biktarvy Sexually Transmitted Diseases Emory University|Centers for Disease Control and Prevention May 20 2021 Phase 4
NCT04762134 Not yet recruiting Drug: Doxycycline Hyclate Bacterial Sexually Transmitted Diseases Jonathan Troy Grennan|Canadian Institutes of Health Research (CIHR)|British Columbia Centre for Disease Control April 1 2021 Phase 2|Phase 3
NCT04597424 Recruiting Drug: Experimental: doxycycline|Biological: Bexsero® vaccine Unsafe Sex|Risk-Taking ANRS Emerging Infectious Diseases January 19 2021 Phase 3
NCT04686799 Recruiting Drug: Doxycycline Hyclate 20 MG Lateral Epicondylitis|Lateral Epicondylitis Left Elbow|Lateral Epicondylitis Right Elbow|Lateral Epicondylitis Unspecified Elbow|Lateral Epicondylitis (Tennis Elbow) Bilateral Hospital for Special Surgery New York December 3 2020 Phase 1
NCT04269434 Active not recruiting Other: No Screening Neisseria Gonorrhoeae Infection|Chlamydia Trachomatis Infection Institute of Tropical Medicine Belgium September 21 2020 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID