Daunorubicin HCl

Catalog No.S3035 Synonyms: Daunomycin HCl

Daunorubicin HCl Chemical Structure

Molecular Weight(MW): 563.98

Daunorubicin HCl inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay.

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In DMSO USD 90 In stock
USD 70 In stock
USD 270 In stock
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Cited by 18 Publications

3 Customer Reviews

  • A) MV411 or B) Kasumi 1 cells were incubated with YM155 (0.25 x IC50) in the presence or absence of i) 0.5 x IC50 or ii) 1 x IC50 of Daunorubicin or iii) 0.5 x IC50 or iv) 1 x IC50 of Cytarabine for 72h. Following 72h treatment, cells were subject to a resazurin reduction assay. Percentages were normalised to DMSO controls. Column, mean of 3 independent experiments; Bars, SEM. *p<0.05, **p<0.01, ***p<0.005. Students t-test. Similar trends were observed with 0.5 x and 1 x IC50 of YM155 in combination with Daunorubicin or Cytarabine (data not shown).

    Leuk Res, 2015, 39(4):435-44.. Daunorubicin HCl purchased from Selleck.

  • IL-17A promotes resistance to daunorubicin. Nalm-6 cells and patient B-ALL cells isolated from 4 B-ALL patients were cultured for 24 h in complete medium with or without IL-17A at 50 ng/ml and subsequently supplemented with daunorubicin at 100, 200, or 400 nM as indicated for 24 h. The cell viability was determined using CCK-8 assay, and apoptosis was determined using Annexin V staining. IL-17A promoted the proliferation and reduced apoptosis of Nalm-6 cells (a, c) and patient B-ALL cells (b, d). Statistical data representing at least 3 independent experiments were shown

    J Transl Med, 2016, 14(1):132. Daunorubicin HCl purchased from Selleck.

  • LKB1 downregulation increased the sensitivity of KG1a cells to chemotherapy using cell viability assays. The viability of LKB1 knockdown cells was decreased more significantly compared with control cells after treatment with daunorubicin. *P<0.01, vs LKB1-KD; **P<0.001, vs LKB1-KD.

    Mol Cell Biochem, 2017, 434(1-2):25-32. Daunorubicin HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Daunorubicin HCl inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay.
Targets
DNA synthesis [1]
(Cell-free assay)
20 nM(Ki)
In vitro

At drug concentrations that reflect the peak plasma concentration after Daunorubicin administration, the primary mechanism is likely to be through interaction with topoisomerase II, which may be a primary triggering event for growth arrest and/or cell killing through a signalling pathway leading to apoptosis, at least in leukemic cells and thymocytes. The quinone structure permits daunorubicin to act as electron acceptors in reactions mediated by oxoreductive enzymes including cytochrome P450 reductase, NADH dehydrogenase, and xanthine oxidase. At Daunorubicin concentrations exceeding approximately 2–4 μM, free radical mediated toxicity and DNA cross-linking may become evident. Daunorubicin inhibits both DNA and RNA syntheses in HeLa cells over a concentration range of 0.2 through 2 μM. Daunorubicin inhibits both DNA syntheses in Ehrlich ascites tumor cells over a concentration range of 4 μM. Daunorubic triggers apoptosis at concentrations of 0.5 and 1 μM in either HL-60 or U-937 human leukemic cells. [1] Daunorubicin stimulates ceramide elevation and apoptosis in P388 and U937 cells through de novo synthesis via activation of the enzyme ceramide synthase. [2] Daunorubicin dose-dependently increases the phosphatidylserine exposure and consequent procoagulant activity of human umbilical vein endothelial cells. Daunorubicin (0.2 mM) significantly enhances the release of endothelial microparticles which are highly procoagulant in human umbilical vein endothelial cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
P388 leukemia cell line MkPBR5l1d3SxeHnjxsBie3OjeR?= M{DSTmNwdXCxdX7kJJdieyCndnHseYF1\WRiZn;yJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KFB|OEigcIV2c2WvaXGgZ4VtdCCuaX7lMEBKSzVyPUOgcm0> MVWxNFQ4QTJ7Nx?=
HL60 cells MlrRSpVv[3Srb36gZZN{[Xl? M3fZdGlvKH[rdILvJIlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IIDyc4xq\mW{YYTpc44hd2ZiSFy2NEBk\WyuczygTWM2OD1yLkCwOUDPxE1? NWrkR4tFOTJ6N{e1PVM>
human epidermoid carcinoma cell line KB-3-1 MW\DfZRwfG:6aXRCpIF{e2G7 NYf2Z5NQUW5idnn0do8h[3m2b4TvfIlkKGGldHn2bZR6KHejczDleoFtfWG2ZXSgeZNqdmdidHjlJIh2dWGwIHXwbYRmem2xaXSgZ4Fz[2mwb33hJINmdGxibHnu[UBMSi1|LUGsJGlEPTB;NzDuUS=> M4WxcVEzPDR|N{[z
human K562 cells NWizepZtS3m2b4TvfIlkyqCjc4PhfS=> MXe3NkBp MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLOVYzKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDYWHQh[XO|YYmsJGdKPTB;Nz60JI5O M4HtZVIxPTlzNke4
Dx5 w/PSC cell lines MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEHndVBVcGViY3;tdI92dmRid3HzJIV3[Wy3YYTl[EBnd3JidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKES6NTD3M3BUSyClZXzsJIxqdmW|IIXzbY5oKE2WVDDhd5NigSxiSVO1NF0yPCCwTR?= NEOyNmEyODZ7OES0PS=>
MDA MB 361(mammary carcinoma cell line) MomyR5l1d3SxeHnjxsBie3OjeR?= NVfUOJJrXGinIHPvcZBwfW6mIIfhd{B1\XO2ZXSg[o9zKGmwdILhZ4VtdHWuYYKgZ5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDIF3CJFM3OSivYX3tZZJ6KGOjcnPpco9u[SClZXzsJIxqdmVrIHX4dJJme3OrbnegUIFkYiBqYnX0ZU1o[WyjY4Tvd4ll[XOnKTygTWM2OD1zNzDuUS=> M3vSblExOzl3NEmw
human MDA-MB-231 cells MkHmR5l1d3SxeHnjxsBie3OjeR?= NGTFPY44OiCq NFLxW4lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvODJizszN M2jaR|E6QTZ6MkW4
MES-SA uterine tumor cell lines M1X0SWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFjwfWpVcGViY3;tdI92dmRid3HzJIV3[Wy3YYTl[EBnd3JidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKE2HUz3TRUB2fGW{aX7lJJR2dW:{IHPlcIwhdGmwZYOgeZNqdmdiTWTUJIF{e2G7LDDJR|UxRTBwMEKxJO69VQ>? MWOxNFY6QDR2OR?=
murine L1210 leukemia cells NWLCWY86TnWwY4Tpc44h[XO|YYm= M3jXcmlvcGmkaYTvdpkh[WO2aY\peJkhf2G|IH3lZZN2emWmIHHnZYlve3RibYXybY5mKExzMkGwJIxmfWunbXnhJINmdGy|LDDJR|UxRTNyIH7N MX[5O|M{PDh|
HL60 cells NI\NS5JHfW6ldHnvckBie3OjeR?= NFvUc2RKdiC4aYTyc{Bkd26lZX70doF1cW:wIILldZVqemWmIITvJIlv\HWlZTDhdI9xfG:|aYOgbY4hUEx4MDDj[YxteyxiQVO1NF0xNjB2IN88US=> M4C2fFEzQDd5NUmz
CCRF-CEM (human lymphoblastic leukemic) cells NXXhUHA6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnrmTY4hfmm2cn:g[5Jwf3SqIHnubIljcXSxcomgZYN1cX[rdImgZYdicW6|dDDDR3JHNUOHTTCobJVu[W5ibIntdIhw[myjc4TpZ{Bt\XWtZX3pZ{kh[2WubIOsJGlFPTB;MD6wOEDPxE1? M4HTRlY6PTFyNEm=
mouse NIH/3T3 cells NFXKZWZEgXSxdH;4bYPDqGG|c3H5 MlS2O|IhcA>? M1LEeWlvfHKrboPpZ{BkgXSxdH;4bYNqfHliYXfhbY5{fCC5aXzkJJR6eGVibX;1d4UhVkmKL{PUN{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMES0JO69VQ>? M{jTfFE5QTV2MESw
human prostate cancer HTB-81 cells MmXkR5l1d3SxeHnjxsBie3OjeR?= MnT6R5l1d3SxeHnjbZR6KHSqYYSgZ4F2e2WmIHGgOVAmKHKnZIXjeIlwdiCrbjDhZpNwemKjbnPlJIF1KDR7MDDucUBqdiCqdX3hckBxem:|dHH0[UBk[W6lZYKgTHRDNThzIHPlcIx{KHW|aX7nJG1VWyCjc4PhfUwhTUN3ME2wMlA2QCEQvF2= NV7QdmdMOTFyMkCyPFU>
human breast carcinoma line cells NHr2OmxHfW6ldHnvckBie3OjeR?= M1\UZ4NwdXCxdX7kJJdieyC2ZYP0[YQhcW5idnn0do8h\m:{IHnubIljcXSrb36gZYN1cX[rdImgZYdicW6|dDDhJJNmdnOrdHn2[UBpfW2jbjDidoVie3RiY3HyZ4lvd22jIHzpcoUh[2WubIOsJGlEPTB;MD6wOkDPxE1? MkjUPVI2QDN3MR?=
MCF-7 cells M3fDRmN6fG:2b4jpZ:Kh[XO|YYm= MUfDc41xd3WwZDD3ZZMhfGW|dHXkJIZweiCrdIOgeI95cWOrdImgZYdicW6|dDDoeY1idiCkcnXhd5Qh[2GwY3XyJINmdGy|KF3DSk04MSxiSVO1NF0xNjB4IN88US=> MUS5OVQ5QDJy
human HT-29 cells NFTxemdEgXSxdH;4bYPDqGG|c3H5 M4TYbVczKGh? NX7RcZFrS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFRvMkmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO|YYmsJGdKPTB;MD6wO{DPxE1? MWGxPVk3QDJ3OB?=
human uterine sarcoma cell line MES-SA NGLtU2lEgXSxdH;4bYPDqGG|c3H5 NWnTS2R7UW5idnn0do8h[3m2b4TvfIlkKGGldHn2bZR6KHejczDleoFtfWG2ZXSgeZNqdmdidHjlJIh2dWGwIIX0[ZJqdmVic3HyZ49u[SClZXzsJIxqdmViTVXTMXNCNCCLQ{WwQVAvODdizszN MXexNlQ1Ozd4Mx?=
human MRC5 cells M1zUeXBzd2yrZnXyZZRqd25iYYPzZZk> M{HjN2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTWLDOUBk\WyuczDifUBZXFRiYYPzZZktKEmFNUC9NE4xPzhizszN MnfoNlU1Pjd{OUG=
MDA435/LCC6 cells MUjQdo9tcW[ncnH0bY9vKGG|c3H5 NEXSe2hCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JG1FSTR|NT;MR2M3KGOnbHzzJIJ6KEWOSWPBMEBKSzVyPUCuNFc6KM7:TR?= MnjHNVcyPTR3MEW=
human A549 cells M3HNcGN6fG:2b4jpZ:Kh[XO|YYm= NUPCPJRkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygS2k2OD1yLkC4JO69VQ>? NVX3bFRSOTl7NkiyOVg>
Jurkat T lymphocytes M2PEfmN6fG:2b4jpZ:Kh[XO|YYm= MlLIR5l1d3SxeHnjbZR6KGGpYXnud5QhUnW{a3H0JHQhdHmvcHjvZ5l1\XNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNFgzPiEQvF2= MXqxOlM3PjZyMh?=
human DU145 cells MX;Qdo9tcW[ncnH0bY9vKGG|c3H5 M4TtOFczKGh? NFP6RpBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTVNVQ2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGlEPTB;MD6wPUDPxE1? NV7pZW1GOjJ{NkCxOlY>
human MDA-MB-231 cells NF[1[WtRem:uaX\ldoF1cW:wIHHzd4F6 NF\XZ|RCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYomgXHRVKGG|c3H5MEBKSzVyPUCuNFkyKM7:TR?= MYWyOVQ3PzJ7MR?=
human H1299 cells MkHnVJJwdGmoZYLheIlwdiCjc4PhfS=> M4DqPGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSEGyPVkh[2WubIOgZpkhYFSWIHHzd4F6NCCLQ{WwQVAvODl4IN88US=> MVSyOVQ3PzJ7MR?=
human HL60 cells NVP4XmV3S3m2b4TvfIlkyqCjc4PhfS=> MVS0PEBp M2DFfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KHS{eYDhckBjdHWnIIP0ZYlvcW6pLXLhd4VlKGinbX;jfZRwdWW2cnnjJIFv[Wy7c3nzMEBIUTVyPUCuNUDPxE1? NXvyZYxtOjV6N{SzN|U>
human LNCAP cells NHP3PJpEgXSxdH;4bYPDqGG|c3H5 NHe0TFA1KGSjeYO= Mkj4R5l1d3O2YYTpZ{Bi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzOR2FRKGOnbHzzJIFnfGW{IESg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6xJO69VQ>? MoH0NlI1QDB2OUW=
NCI-H69 cell line M320RXBzd2yrZnXyZZRqd25iYYPzZZk> M1S3O2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\yC|ZX7zbZRqfmViTlPJMWg3QSClZXzsJIxqdmViYomgRYxidWG{IFLseYUh[XO|YYmsJGlEPTB;MD6xJO69VQ>? NHHyR5YyPzJ6NkO5Ny=>
human A2780 cells MXzDfZRwfG:6aXRCpIF{e2G7 NX3peXhWPzJiaB?= M1j2bWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE4yOTd2OTFOwG0> MV:xO|g6ODB7NB?=
human HT-29 cells M1;qb3Bzd2yrZnXyZZRqd25iYYPzZZk> M3;QeFk3KGh? MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC5zM{Og{txO M{DDXFI4Pzh2NEm=
B16 mouse melanoma cells NUnWbodIS3m2b4TvfIlkyqCjc4PhfS=> MmfCR5l1d3SxeHnjbZR6KHSqYYSgZ4F2e2WmIHGgOVAmKHKnZIXjeIlwdiCrbjDhZpNwemKjbnPlJIF1KDR7MDDucUBqdiCEMU[gcY92e2VibXXsZY5wdWFiY3XscJMhfXOrbnegUXRUKGG|c3H5MEBGSzVyPUCuNVYh|ryP NHXMc|kyOTB{MEK4OS=>
human SKMES1 cells NWDi[XlvWHKxbHnm[ZJifGmxbjDhd5NigQ>? NVXaUnlMPzJiaB?= M2fvWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1vNSXMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGlEPTB;MD6xO{DPxE1? NG\ydYEzOjJ4MEG2Oi=>
human fibroblast (NHF) cells NV[1WlNuS3m2b4TvfIlkyqCjc4PhfS=> M332bWN6fG:2b4jpZ4l1gSC2aHH0JINifXOnZDDhJFUxLSC{ZXT1Z5Rqd25iaX6gZYJ{d3KkYX7j[UBifCB2OUCgco0hcW5ibn;ycYFtKGi3bXHuJIZq[nKxYnzhd5QhME6KRjmgZ4VtdHNidYPpcochVVSVIHHzd4F6NCCHQ{WwQVAvOjJizszN MYixNVAzODJ6NR?=
mouse L1210 cells NE\IS|ZHfW6ldHnvckBie3OjeR?= MkjOTY5pcWKrdHnvckBw\iCUTlGgd5lvfGinc3nzJIlvKG2xdYPlJGwyOjFyIHPlcIx{NCCHREWwQVAvOjNizszN M3TLZVY6ODl7OR?=
human NCI-H460 cells MmL6SpVv[3Srb36gZZN{[Xl? NGPhUGVCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUmNKNUh2NkCgZ4VtdHNuIFnDOVA:OC5|ODFOwG0> NX7qXHpNOjF3OUmwNFA>
human MCF7 cells M2r6SWN6fG:2b4jpZ:Kh[XO|YYm= NGroOpI1KGSjeYO= NH\XTZREgXSxc4TheIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0JIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUCuOEDPxE1? M2LHNlIzPDhyNEm1
human SF268 cells Mlq2SpVv[3Srb36gZZN{[Xl? NHjSZ4NCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gV2YzPjhiY3XscJMtKEmFNUC9NE43KM7:TR?= NX;Rc3d7OjF3OUmwNFA>
human KB cells M4DTemN6fG:2b4jpZ:Kh[XO|YYm= NFnZR|A1QCCq M3PnXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD62OEDPxE1? Ml7yNlA{PjNzNEK=
human HepG2 cells NUn4OJkyS3m2b4TvfIlkyqCjc4PhfS=> M3TxOlI1KGh? NVK0c2dxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0xNjdizszN MkHyNlExQTRyNEm=
mouse L5178Y cells MVXDfZRwfG:6aXRCpIF{e2G7 NWjXZ3hlOjRiaB?= NFG0ToNEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBNPTF5OGmgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjd{IN88US=> NXLVcFgxOjNzOUm0O|k>
human MCF7 cells NHvPfpBEgXSxdH;4bYPDqGG|c3H5 M4DDZ|czKGh? MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDYWHQh[XO|YYmsJGdKPTB;MD64OkDPxE1? M3v5bVIxPTlzNke4
human MRC5 cells M1\GNnBzd2yrZnXyZZRqd25iYYPzZZk> NWiycGtZSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNVmM2KGOnbHzzMEBKSzVyPUCuPFgh|ryP NXrzOGRnOjF3OUmwNFA>
MDA435/LCC6 cells MmrmVJJwdGmoZYLheIlwdiCjc4PhfS=> M{HoPGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcZVtfGmmcoXnJJJme2m|dHHueEBOTEF2M{WvUGNEPiClZXzsd{BjgSCHTFnTRUwhUUN3ME2wMlk4PyEQvF2= M2DZcFE4OTV2NUC1
mouse NIH/3T3 cells NEPPOGZEgXSxdH;4bYPDqGG|c3H5 NIrzOms4OiCq NHPBRlhKdnS{aX7zbYMh[3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhVkmKL{PUN{Bk\WyuczDveoVz\XiycnXzd4lv\yCPRGKxJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT6wNUDPxE1? MXyxPFk2PDB2MB?=
human Detroit 551 cells NFfNRoxEgXSxdH;4bYPDqGG|c3H5 NUDTXWZ2PzJiaB?= MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDE[ZRzd2m2IEW1NUBk\WyuczDh[pRmeiB5MjDodpMh[nliWGTUJIF{e2G7LDDHTVUxRTFwN{eg{txO MXSyNFU6OTZ5OB?=
mouse DA-3 cells M4L5TGN6fG:2b4jpZ:Kh[XO|YYm= MmrxNlQhcA>? M1HtOmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGRCNTNiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JHhVXCCjc4PhfUwhUUN3ME2xMlkh|ryP M3\WU|I1QTByNk[4
african green monkey Vero cells NWPIVJZqS3m2b4TvfIlkyqCjc4PhfS=> NIHKOmg{KGSjeYO= Moe3R5l1d3SxeHnjbZR6KGGpYXnud5Qh[W[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDh[pRmeiB|IHThfZMh[nliTWTTJIF{e2G7LDDJR|UxRTJwNTFOwG0> NYX2d4Y5OjFyOUSwOFk>
Dx5 cell lines MmDOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnvJWIhmKGOxbYDveY5lKHejczDleoFtfWG2ZXSg[o9zKHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kBFgDViY3XscEBtcW6nczD1d4lv\yCPVGSgZZN{[XluIHnjOVA:OiEQvF2= M2f5RVExPjl6NES5
human HeLa3 cell line NHLmPWlEgXSxdH;4bYPDqGG|c3H5 NEjrUZFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjMzDj[YxtKGyrbnWgZpkhVVSWIHHzd4F6NCCLQ{WwQVIvQTZizszN NUj2[WozOTZ7NEKwN|c>
mouse L5178Y cells MkT6R5l1d3SxeHnjxsBie3OjeR?= MoLoNlQhcA>? MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDMOVE4QFliY3XscJMh\XiycnXzd4lv\yCyLXfwJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Nz63N{DPxE1? NFPETpQzOzF7OUS3PS=>
human CRL7761 cells MYjDfZRwfG:6aXRCpIF{e2G7 M1;1UFMh\GG7cx?= M3jzVmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNTVDd5NkGgZ4VtdHNiYX\0[ZIhOyCmYYnzJIJ6KE2WUzDhd5NigSxiSVO1NF0yOC53IN88US=> NGT2Zo4zOTB7NEC0PS=>
human A2780/ADR cells NE\oUZZEgXSxdH;4bYPDqGG|c3H5 NH\oOWo4OiCq M2LpeGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyL1HEVkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF6Lk[yNFkh|ryP NGO3OZUyPzh7MEC5OC=>
human SKOV3 cells MnjkR5l1d3SxeHnjxsBie3OjeR?= MWKyOEBp NFfER5REgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0:YMzDj[YxteyCjZoTldkAzPCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwQVIxNjZizszN MWGyOFkxODZ4OB?=
human ES2 cells MnW2R5l1d3SxeHnjxsBie3OjeR?= M4DyPVI1KGh? NWTTfVhES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTVN{IHPlcIx{KGGodHXyJFI1KGi{czDifUBZXFRiYYPzZZktKEmFNUC9NlUvPSEQvF2= NID5[mkzPDlyME[2PC=>
CEM cells M4PGVGN6fG:2b4jpZ:Kh[XO|YYm= NFzZN3A4OiCq MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBETU1iY3XscJMh[W[2ZYKgO|IhcHK| NXGyeXV5OTd4MEizPVY>
HOS cells NIrjdW1EgXSxdH;4bYPDqGG|c3H5 NFu5UlA4OiCq M4mwUWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiRUzDj[YxteyCjZoTldkA4OiCqcoO= MmTzNVc3ODh|OU[=
mouse DA-3 cells MnW2R5l1d3SxeHnjxsBie3OjeR?= M2HuTFIxKM7:TR?= NFPReGI3OCCvaX7z M2PZOWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGRCNTNiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHH0JFIxKHWPIHHmeIVzKDZyIH3pcpMh[nliWGTUJIF{e2G7 Mmm1NlQ6ODB4Nki=
human G361 cells M1HuXGN6fG:2b4jpZ:Kh[XO|YYm= M1K2UFczKGh? NV[1W2VMS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTzN4MTDj[YxteyCjZoTldkA4OiCqcoO= NV60ZW1UOTd4MEizPVY>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Chk1 / Chk1 / p-ATR / ATR ; 

PubMed: 26727128     


After Caco-2 cells were treated with or without 1 μ M daunorubicin, whole cell extracts were collected at 1, 3, 6, 12, 24, and 48 h and immunoblotted for Chk1, p-Chk1, ATR, and p-ATR. β-actin served as the loading control.

26727128
Immunofluorescence
p-Chk1; 

PubMed: 26727128     


Caco-2 cells were treated with or without 1 μ M daunorubicin for 48 h, and then fixed and stained with anti-p-Chk1 antibody and DAPI. Images were collected under a fluorescence microscope. Scale bar = 10 μm. 

26727128
Growth inhibition assay
Cell viability; 

PubMed: 30745844     


(A) Viability of AML cell lines under different concentrations of Dnr according to the CCK-8 assay. (B) IC50 of AML cell lines calculated according to the result of the CCK-8 assay.

30745844

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL (177.31 mM)
Water 100 mg/mL (177.31 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 563.98
Formula

C27H29NO10 . HCl

CAS No. 23541-50-6
Storage powder
in solvent
Synonyms Daunomycin HCl

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03878927 Recruiting Drug: CPX-351|Drug: Gemtuzumab Ozogamicin Acute Myeloid Leukemia Weill Medical College of Cornell University|Jazz Pharmaceuticals|Pfizer August 23 2019 Phase 1
NCT03896269 Recruiting Drug: Liposome-encapsulated Daunorubicin-Cytarabine Blasts 10-19 Percent of Bone Marrow Nucleated Cells|Blasts More Than 5 Percent of Bone Marrow Nucleated Cells|High Risk Chronic Myelomonocytic Leukemia|Recurrent Chronic Myelomonocytic Leukemia|Recurrent High Risk Myelodysplastic Syndrome|Refractory Chronic Myelomonocytic Leukemia|Refractory High Risk Myelodysplastic Syndrome M.D. Anderson Cancer Center|National Cancer Institute (NCI) May 14 2019 Phase 1
NCT02914977 Active not recruiting Drug: Daunorubicin Acute Lymphocytic Leukemia|Acute Myeloid Leukemia Tara Lin|University of Kansas Medical Center November 2016 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID