Daunorubicin (RP 13057) HCl

Catalog No.S3035 Synonyms: Daunomycin HCl, Rubidomycin HCl

For research use only.

Daunorubicin HCl (Daunomycin, RP 13057, Rubidomycin) inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay. Daunorubicin is a topoisomerase II inhibitor that induces apoptosis.

Daunorubicin (RP 13057) HCl Chemical Structure

CAS No. 23541-50-6

Selleck's Daunorubicin (RP 13057) HCl has been cited by 53 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Daunorubicin HCl (Daunomycin, RP 13057, Rubidomycin) inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay. Daunorubicin is a topoisomerase II inhibitor that induces apoptosis.
Targets
DNA synthesis [1]
(Cell-free assay)
20 nM(Ki)
In vitro

At drug concentrations that reflect the peak plasma concentration after Daunorubicin administration, the primary mechanism is likely to be through interaction with topoisomerase II, which may be a primary triggering event for growth arrest and/or cell killing through a signalling pathway leading to apoptosis, at least in leukemic cells and thymocytes. The quinone structure permits daunorubicin to act as electron acceptors in reactions mediated by oxoreductive enzymes including cytochrome P450 reductase, NADH dehydrogenase, and xanthine oxidase. At Daunorubicin concentrations exceeding approximately 2–4 μM, free radical mediated toxicity and DNA cross-linking may become evident. Daunorubicin inhibits both DNA and RNA syntheses in HeLa cells over a concentration range of 0.2 through 2 μM. Daunorubicin inhibits both DNA syntheses in Ehrlich ascites tumor cells over a concentration range of 4 μM. Daunorubic triggers apoptosis at concentrations of 0.5 and 1 μM in either HL-60 or U-937 human leukemic cells. [1] Daunorubicin stimulates ceramide elevation and apoptosis in P388 and U937 cells through de novo synthesis via activation of the enzyme ceramide synthase. [2] Daunorubicin dose-dependently increases the phosphatidylserine exposure and consequent procoagulant activity of human umbilical vein endothelial cells. Daunorubicin (0.2 mM) significantly enhances the release of endothelial microparticles which are highly procoagulant in human umbilical vein endothelial cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA MB 361(mammary carcinoma cell line) NXThe5dRS3m2b4TvfIlkyqCjc4PhfS=> NVO3bGZFXGinIHPvcZBwfW6mIIfhd{B1\XO2ZXSg[o9zKGmwdILhZ4VtdHWuYYKgZ5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDIF3CJFM3OSivYX3tZZJ6KGOjcnPpco9u[SClZXzsJIxqdmVrIHX4dJJme3OrbnegUIFkYiBqYnX0ZU1o[WyjY4Tvd4ll[XOnKTygTWM2OD1zNzDuUS=> M4O1[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyM{m1OFkxLz5zMEO5OVQ6ODxxYU6=
P388 leukemia cell line Mn3OR5l1d3SxeHnjxsBie3OjeR?= NUjYPFBLS2:vcH;1coQhf2G|IHX2ZYx2[XSnZDDmc5Ih[3m2b4TvfIlkcXS7IHHnZYlve3RiUEO4PEBt\XWtZX3pZUBk\WyuIHzpcoUtKEmFNUC9N{BvVQ>? NULjb5ZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC0O|kzQTdpPkGwOFc6Ojl5PD;hQi=>
Dx5 w/PSC cell lines MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVfUbIUh[2:vcH;1coQhf2G|IHX2ZYx2[XSnZDDmc5IhfGinIHfyc5d1cCCrbnjpZol1cW:wIH;mJGR5PSC5L2DTR{Bk\WyuIHzpcoV{KHW|aX7nJG1VXCCjc4PhfUwhUUN3ME2xOEBvVQ>? M1XaWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNkm4OFQ6Lz5zME[5PFQ1QTxxYU6=
MES-SA uterine tumor cell lines NYHL[IxST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVHwOVd{XGinIHPvcZBwfW6mIIfhd{BmfmGudXH0[YQh\m:{IITo[UBoem:5dHigbY5pcWKrdHnvckBw\iCPRWOtV2EhfXSncnnu[UB1fW2xcjDj[YxtKGyrbnXzJJV{cW6pIF3UWEBie3OjeTygTWM2OD1yLkCyNUDPxE1? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODZ7OES0PUc,OTB4OUi0OFk9N2F-
Dx5 cell lines MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIXHbXNVcGViY3;tdI92dmRid3HzJIV3[Wy3YYTl[EBnd3JidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKES6NTDj[YxtKGyrbnXzJJV{cW6pIF3UWEBie3OjeTygbYM2OD1{IN88US=> M13PSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNkm4OFQ6Lz5zME[5PFQ1QTxxYU6=
human epidermoid carcinoma cell line KB-3-1 M1nuZWN6fG:2b4jpZ:Kh[XO|YYm= MlzBTY4hfmm2cn:gZ5l1d3SxeHnjJIFkfGm4aYT5JJdieyCndnHseYF1\WRidYPpcochfGinIHj1cYFvKGWyaXTldo1wcWRiY3HyZ4lvd22jIHPlcIwhdGmwZTDLRk0{NTFuIFnDOVA:PyCwTR?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjR2M{e2N{c,OTJ2NEO3OlM9N2F-
human uterine sarcoma cell line MES-SA M3;X[2N6fG:2b4jpZ:Kh[XO|YYm= MU\JckB3cXS{bzDjfZRwfG:6aXOgZYN1cX[rdImge4F{KGW4YXz1ZZRm\CC3c3nu[{B1cGViaIXtZY4hfXSncnnu[UB{[XKlb33hJINmdGxibHnu[UBOTVNvU1GsJGlEPTB;MD6wO{DPxE1? NHu3[GU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkS0N|c3Oyd-MUK0OFM4PjN:L3G+
Jurkat T lymphocytes NYnDcWM3S3m2b4TvfIlkyqCjc4PhfS=> MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBLfXKtYYSgWEBtgW2yaH;jfZRmeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6wPFI3KM7:TR?= NHn4Xpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkO2OlYxOid-MU[zOlY3ODJ:L3G+
human HeLa3 cell line NELOdZJEgXSxdH;4bYPDqGG|c3H5 NUjJb3psS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYUOgZ4VtdCCuaX7lJIJ6KE2WVDDhd5NigSxiSVO1NF0zNjl4IN88US=> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl2MkCzO{c,OTZ7NEKwN|c9N2F-
NCI-H69 cell line NWHORZM6WHKxbHnm[ZJifGmxbjDhd5NigQ>? MmXRRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoKHOnboPpeIl3\SCQQ1mtTFY6KGOnbHygcIlv\SCkeTDBcIFu[XJiQnz1[UBie3OjeTygTWM2OD1yLkGg{txO MmDhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd{OE[zPVMoRjF5Mki2N|k{RC:jPh?=
L1210 MofVSpVv[3Srb36gZZN{[Xl? M2\xdmlvcGmkaYTpc44hd2ZiUl7BJJN6dnSqZYPpd{BqdiCvb4Xz[UBNOTJzMDDj[YxteyxiRVS1NEA:KDBwMzFOwG0v MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek81OjNzOEGnQlQzOzF6MUyvZV4>
L1210 Mn3LSpVv[3Srb36gZZN{[Xl? MWDJcohq[mm2aX;uJI9nKESQQTDzfY51cGW|aYOgbY4hdW:3c3WgUFEzOTBiY3XscJMtKEWGNUCgQUAyKM7:TT6= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek81OjNzOEGnQlQzOzF6MUyvZV4>
L1210 NYmyclZWTnWwY4Tpc44h[XO|YYm= MUHJcohq[mm2aX;uJI9nKFKQQTDzfY51cGW|aYOgbY4hdW:3c3WgUFEzOTBiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBcO0ifdYLp[Ilv\SCrbnPvdpBwemG2aX;uMEBGTDVyIE2gNE4{KM7:TT6= NYDlU2pXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxNEmwOVM4Lz52OUC1N|c9N2F-
L1210 MYPGeY5kfGmxbjDhd5NigQ>? NXXUdlRYUW6qaXLpeIlwdiCxZjDEUmEhe3mwdHjld4l{KGmwIH3veZNmKExzMkGwJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiW{PIYZRpgW2rZHnu[UBqdmOxcoDvdoF1cW:wLDDFSFUxKD1iMD60JO69VS5? M{DQe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzR7MEWzO{c,PDlyNUO3QE9iRg>?
L1210 NUfheVVWTnWwY4Tpc44h[XO|YYm= MYXJcohq[mm2aX;uJI9nKFt|SG31dollcW6nIHnuZ49zeG:{YYTpc44hcW62bzDwdo9tcW[ncnH0bY5oKExzMkGwJINmdGy|LDDFSFUxKD1iMD6zN{DPxE1w NGToVHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi94N{G2OFAyLz54N{G2OFAyRC:jPh?=
L1210 NVXa[4o4TnWwY4Tpc44h[XO|YYm= MoTxTY5pcWKrdHnvckBw\iCdM1jdeIh6dWmmaX7lJIlv[2:{cH;yZZRqd25iaX70c{Bxem:uaX\ldoF1cW6pIFyxNlExKGOnbHzzMEBGTDVyIE2gNE43PiEQvF2u NYrXXJh5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxNkexOlQxOSd-NkexOlQxOTxxYU6=
L1210 NG[zZoFHfW6ldHnvckBie3OjeR?= NVW4VZlKTG:|ZTDy[ZF2cXKnZDD0c{BqdmirYnn0JJRp\SCUTlGgd5lvfGinc3nzJIlvKGyndXvlcYliKExzMkGwJINmdGy|LDDFSFUxKD1iMD6zJO69VS5? NHHSbFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi95MkWyPVc3Lz55MkWyPVc3RC:jPh?=
L1210 NWfIO2V3TnWwY4Tpc44h[XO|YYm= NIO2ZVJFd3OnIILldZVqemWmIITvJIlvcGmkaYSgeIhmKESQQTDzfY51cGW|aYOgbY4hdGW3a3XtbYEhVDF{MUCgZ4VtdHNuIFXEOVAhRSBzIN88UU4> M1\aVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd{NUK5O|YoRjd{NUK5O|Y9N2F-
MCF7 MV;DfZRwfG:6aXPpeJkh[XO|YYm= NWjLcGI{PDhiaILz MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlIzKM7:TT6= NIjVeHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G5NFQ1Pyd-MUexPVA1PDd:L3G+
MDA-MB-231 MWDDfZRwfG:6aXPpeJkh[XO|YYm= MnzPOFghcHK| NGTS[WtEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlI2KM7:TT6= NIDI[5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G5NFQ1Pyd-MUexPVA1PDd:L3G+
HeLa MWDDfZRwfG:6aXPpeJkh[XO|YYm= NVnuWnR6PDhiaILz MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlMzKM7:TT6= M17kcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUmwOFQ4Lz5zN{G5NFQ1PzxxYU6=
PC-3 MoCxR5l1d3SxeHnjbZR6KGG|c3H5 M{HlXVQ5KGi{cx?= MmHMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGMuOyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE41PCEQvF2u Mn\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzOUC0OFcoRjF5MUmwOFQ4RC:jPh?=
Yoshida sarcoma tumor cells MnjvSpVv[3Srb36gZZN{[Xl? NI\RTplEd26lZX70doF1cW:wIHnubIljcXSrbnegX|NJZVSmUjDpcoNwenCxcnH0bY9vKGmwIGnvd4hq\GFic3HyZ49u[SC2dX3vdkBk\WyuczDpckB3cXS{bzDpckBz[XS|LDDJR|UxKD1iMD6wNkDPxE1w MmDIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTN{Mkm4Okc,OTN{Mkm4Olww[T5?
HL60 MYjBdI9xfG:|aYOgZZN{[Xl? NInPbWxKdiC4aYTyc{Bkd26lZX70doF1cW:wIILldZVqemWmIITvJIlv\HWlZTDhdI9xfG:|aYOgbY4hUEx4MDDj[YxteyxiQVO1NEA:KDBwMESg{txONg>? M1q4[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEe3OVk{Lz5zMki3O|U6OzxxYU6=
HL60R MWfBdI9xfG:|aYOgZZN{[Xl? MWrJckB3cXS{bzDjc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIHnu[JVk\SCjcH;weI9{cXNiaX6gTGw3OFJiY3XscJMtKEGFNUCgQUAyOCEQvF2u Mo\uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ6N{e1PVMoRjF{OEe3OVk{RC:jPh?=
HL60 M3rCPGN6fG:2b4jpZ4l1gSCjc4PhfS=> NIXRU4ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGGodHXyJFQ5KGi{czDifUB1enmyYX6gZox2\SCneHPseZNqd25ibXX0bI9lNCCDQ{WwJF0hOC5zIN88UU4> NInFWFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEOzPVQ2PSd-MUizN|k1PTV:L3G+
HL60 MWrBdI9xfG:|aYOgZZN{[Xl? MYDJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIH31cJRq\HK3ZzDy[ZNqe3SjboSgbJVu[W5iSFy2NEBk\WyuczDh[pRmeiB2ODDodpMtKEGFNUCgQUA{QCEQvF2u Ml34QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|M{m0OVUoRjF6M{O5OFU2RC:jPh?=
HTB-81 NF7rZWVEgXSxdH;4bYNqfHliYYPzZZk> M1f1SmN6fG:2b4jpZ4l1gSC2aHH0JINifXOnZDDhJFUxLSC{ZXT1Z5Rqd25iaX6gZYJ{d3KkYX7j[UBifCB2OUCgco0hcW5iaIXtZY4heHKxc4TheIUh[2GwY3XyJGhVSi16MTDj[YxteyC3c3nu[{BOXFNiYYPzZZktKEWFNUCgQUAxNjB3ODFOwG0v NX35U3ZXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUGwNlAzQDVpPkGxNFIxOjh3PD;hQi=>
B16 M4HlWGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGDpSFBEgXSxdH;4bYNqfHlidHjheEBk[XW|ZXSgZUA2OCVicnXkeYN1cW:wIHnuJIFje2:{YnHuZ4Uh[XRiNEmwJI5uKGmwIFKxOkBud3W|ZTDt[Yxidm:vYTDj[YxteyC3c3nu[{BOXFNiYYPzZZktKEWFNUCgQUAxNjF4IN88UU4> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTB{MEK4OUc,OTFyMkCyPFU9N2F-
fibroblast (NHF) cells MWfDfZRwfG:6aXPpeJkh[XO|YYm= MXXDfZRwfG:6aXPpeJkhfGijdDDjZZV{\WRiYTC1NEUhemWmdXP0bY9vKGmwIHHid49z[mGwY3WgZZQhPDlyIH7tJIlvKG6xcn3hcEBpfW2jbjDmbYJzd2KuYYP0JEhPUEZrIHPlcIx{KHW|aX7nJG1VWyCjc4PhfUwhTUN3MDC9JFAvOjJizszNMi=> MkDxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTFyMkCyPFUoRjFzMEKwNlg2RC:jPh?=
L1210 Mn3qSpVv[3Srb36gZZN{[Xl? MkDoN{BpenN? MnnLTY5pcWKrdHnvckBw\iCGTlGgd5lvfGinc3nzJIlvKG2xdYPlJGwyOjFyIHPlcIx{KHC{ZXnuZ5Vj[XSnZDDmc5IhOyCqcoOg[o9tdG:5ZXSgZpkhYzOKXT30bJlucWSrbnWgZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiMTDodkBjgSC|Y3nueIltdGG2aX;uJJNx\WO2cn;t[ZRzcWNiYX7hcJl{cXNuIFXEOVAhRSByLkOg{txONg>? NUXzXFdbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2N|U6Lz5zN{[zOVk9N2F-
L1210 MVzGeY5kfGmxbjDhd5NigQ>? NH;ae4E{KGi{cx?= M1m4[2lvcGmkaYTpc44hd2ZiUl7BJJN6dnSqZYPpd{BqdiCvb4Xz[UBNOTJzMDDj[YxteyCycnXpcoN2[mG2ZXSg[o9zKDNiaILzJIZwdGyxd3XkJIJ6KFt|SG2teZJq\GmwZTDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiBzIHjyJIJ6KHOlaX70bYxt[XSrb36gd5Bm[3S{b33leJJq[yCjbnHsfZNqeyxiRVS1NEA:KDBwMzFOwG0v MoOzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4M{W5K|4yPzZ|NUm8M4E,
L1210 MV\GeY5kfGmxbjDhd5NigQ>? MVPJcohq[mm2aX;uJI9nKFKQQTDzfY51cGW|aYOgbY4hdW:3c3WgUFEzOTBiY3XscJMtKEWGNUCgQUAxNjN|IN88UU4> M3fYOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzR{M{G4Nkc,PDJ|MUiyQE9iRg>?
L1210 NH3vXHlHfW6ldHnvckBie3OjeR?= NHHLcZJKdmirYnn0bY9vKG:oIFTORUB{gW62aHXzbZMhcW5ibX;1d4UhVDF{MUCgZ4VtdHNuIFXEOVAhRSByLk[2JO69VS5? NGH6SYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi92MkOxPFIoRjR{M{G4Nlww[T5?
L1210 MYfGeY5kfGmxbjDhd5NigQ>? MmrBTY5pcWKrdHnvckBw\iCUTlGgd5lvfGinc3nzJIlvKG2xdYPlJGwyOjFyIHPlcIx{NCCHREWwJF0hOC5|MzFOwG0v M1LVUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzR7MEWzOkc,PDlyNUO2QE9iRg>?
L1210 NH\NfGJHfW6ldHnvckBie3OjeR?= M2H2SWlvcGmkaYTpc44hd2ZiRF7BJJN6dnSqZYPpd{BqdiCvb4Xz[UBNOTJzMDDj[YxteyxiRVS1NEA:KDBwNk[g{txONg>? NX;JVm9iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxNEmwOVM3Lz52OUC1N|Y9N2F-
L1210 NUjycFBbTnWwY4Tpc44h[XO|YYm= M{nUSmlvcGmkaYTpc44hd2ZiUl7BJJN6dnSqZYPpd{BqdiCvb4Xz[UBNOTJzMDDj[YxteyxiRVS1NEA:KDBwMkOg{txONg>? NILtVZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi94OUC5PVkoRjZ7MEm5PVww[T5?
L1210 MVLGeY5kfGmxbjDhd5NigQ>? NILNZYxKdmirYnn0bY9vKG:oIFTORUB{gW62aHXzbZMhcW5ibX;1d4UhVDF{MUCgZ4VtdHNuIFXEOVAhRSByLkS4JO69VS5? NHfiR5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi94OUC5PVkoRjZ7MEm5PVww[T5?
L1210 MWPGeY5kfGmxbjDhd5NigQ>? NEHJXHlEd22yb4Xu[EB4[XNiZY\hcJVifGWmIH\vdkB1cGViNUClJIlvcGmkaYTpc44hd2ZidHjlJIlv[2:{cH;yZZRqd25ib3[gX|NJZS1idHj5cYllcW6nIHnuJJRp\SCUTlGgLIlvcGmkaYTpc44hd2Zic4nueIhme2m|IHnuJIxmfWunbXnhJGwyOjFyIHPlcIx{MSxiRVS1NEA:KDBwM{Og{txONg>? MkDWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzd4MUOyOUc,Ozd4MUOyOVww[T5?
L1210 MnrFSpVv[3Srb36gZZN{[Xl? MoX0R49ueG:3bnSge4F{KGW4YXz1ZZRm\CCob4KgeIhmKDVyJTDpcohq[mm2aX;uJI9nKHSqZTDpcoNwenCxcnH0bY9vKG:oIGuzTH0uKHSqeX3p[Ilv\SCrbjD0bIUhTE6DIDjpcohq[mm2aX;uJI9nKHO7boTo[ZNqeyCrbjDs[ZVs\W2rYTDMNVIyOCClZXzsd{ktKEWGNUCgQUAxNjZ4IN88UU4> NFPG[m09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|N{[xN|I2Lz5|N{[xN|I2RC:jPh?=
L1210 NYrySZY4TnWwY4Tpc44h[XO|YYm= MkXPR49v[2WwdILheIlwdiCxZjDkdpVoKG6nY3Xzd4FzgSC2bzDy[YR2[2ViYomgOVAmKHSqZTDpcoNwenCxcnH0bY9vKG:oIITybZRq[XSnZDD1dollcW6nIHnuJJRp\SCUTlGgc4Yh[WO2aY\lcJkh\3Kxd3nu[{BNOTJzMDDj[YxteyxiRVS1NEA:KDBwM{Og{txONg>? MmLkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPzB6NkixOkc,PzB6NkixOlww[T5?
L1210 NUjySFFXTnWwY4Tpc44h[XO|YYm= MnHxR49v[2WwdILheIlwdiCxZjDkdpVoKG6nY3Xzd4FzgSC2bzDy[YR2[2ViYomgOVAmKHSqZTDpcoNwenCxcnH0bY9vKG:oIITybZRq[XSnZDD0bJlucWSrbnWgbY4hfGinIFTORUBw\iCjY4TpeoVtgSCpcn;3bY5oKExzMkGwJINmdGy|LDDFSFUxKD1iMD62OkDPxE1w NUPqOoI6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxN{C4OlgyPid-N{C4OlgyPjxxYU6=
NCI60 NIHM[mdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX;Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDOR2k3OCClZXzsd{whT0l3MDC9JFAvODR2IN88UU4> NX;aW4RvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OFQ3OTJpPkG5PVQ1PjF{PD;hQi=>
MDA-MB-231 NIjnfI5EgXSxdH;4bYNqfHliYYPzZZk> NYLZPFBTPzJiaILz M4nxXGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUCgQUAxNjB{IN88UU4> NIXlSG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2PFI2QCd-MUm5OlgzPTh:L3G+
HT-29 MYPDfZRwfG:6aXPpeJkh[XO|YYm= MWe3NkBpenN? M2HMU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFczKGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDHTVUxKD1iMD6wO{DPxE1w NUXDbW1DRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OlgzPThpPkG5PVY5OjV6PD;hQi=>
A549 NETtTmdEgXSxdH;4bYNqfHliYYPzZZk> MWS3NkBpenN? MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwJF0hOC5yODFOwG0v NGnocJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2PFI2QCd-MUm5OlgzPTh:L3G+
K562 NWq4NZFLS3m2b4TvfIlkcXS7IHHzd4F6 MlP4O|IhcHK| M1[3c2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGs2PjJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHhVXCCjc4PhfUwhT0l3MDC9JFAvODB5NDFOwG0v NWHxOmJYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1PVE3PzhpPkKwOVkyPjd6PD;hQi=>
NCI-H460 M2XQXWN6fG:2b4jpZ4l1gSCjc4PhfS=> M2j0UlczKGi{cx?= MoPmR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUmNKNUh2NkCgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFiWVDDhd5NigSxiR1m1NEA:KDBwM{ig{txONg>? M{nzeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUmxOlc5Lz5{MEW5NVY4QDxxYU6=
SF268 M{C0V2N6fG:2b4jpZ4l1gSCjc4PhfS=> M1TnZVczKGi{cx?= NYfrcnN3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0Z{NkigZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFiWVDDhd5NigSxiR1m1NEA:KDBwNjFOwG0v MkfVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3OUG2O|goRjJyNUmxOlc5RC:jPh?=
MCF7 M1HpNWN6fG:2b4jpZ4l1gSCjc4PhfS=> MW[3NkBpenN? MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDYWHQh[XO|YYmsJGdKPTBiPTCwMlg3KM7:TT6= MnHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3OUG2O|goRjJyNUmxOlc5RC:jPh?=
Detroit 551 MX;DfZRwfG:6aXPpeJkh[XO|YYm= NX7VUIIzPzJiaILz MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDE[ZRzd2m2IEW1NUBk\WyuczDh[pRmeiB5MjDodpMh[nliWGTUJIF{e2G7LDDHTVUxKD1iMT63O{DPxE1w NYjWVllHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1PVE3PzhpPkKwOVkyPjd6PD;hQi=>
HL60 NXP4XndOS3m2b4TvfIlkcXS7IHHzd4F6 MXS0PEBpenN? NYfReHkyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MDDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nlidIL5dIFvKGKudXWgd5RicW6rbnetZoF{\WRiaHXtc4N6fG:vZYTybYMh[W6jbInzbZMtKEeLNUCgQUAxNjFizszNMi=> NX21fY9mRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4O|Q{OzVpPkK1PFc1OzN3PD;hQi=>
HL60R NGruTJdEgXSxdH;4bYNqfHliYYPzZZk> MYW0PEBpenN? NVLDV3VNS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MGKgZ4VtdHNiZYjwdoV{e2mwZzDwMYdtgWOxcILveIVqdiCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nlidIL5dIFvKGKudXWgd5RicW6rbnetZoF{\WRiaHXtc4N6fG:vZYTybYMh[W6jbInzbZMtKEeLNUCgQUA{QCEQvF2u NH21W2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi3OFM{PSd-MkW4O|Q{OzV:L3G+
P388 MljZRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M4\CVlQ5KGi{cx?= MoPzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCSM{i4JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkCyPEDPxE1w MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd6NES5K|4zPzd6NES5QE9iRg>?
HT-29 MUPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NHvEU4g6PiCqcoO= NYXCbYFySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4yOzNizszNMi=> M4LmbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{i0OFkoRjJ5N{i0OFk9N2F-
HeLa MoLZSpVv[3Srb36gZZN{[Xl? NWDUV3dsXFChVGLBUnNRV1KWRWK6JIlvcGmkaYTpc44hd2ZiRUKxO4JmfGGJIIXweIFs\SBqRUKxO4JmfGGJOjCwMlA2KHWPKTDpckBu\W2kcnHu[UB3\XOrY3zlJIZzd21iTWLQNU1mgHC{ZYPzbY5oKEinTHGgZ4VtdHNuIFnDOVAhRSB6IN88UU4> M1rrWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh4MkG2OFQoRjh4MkG2OFQ9N2F-
breast carcinoma line cells NV\Yd2NFTnWwY4Tpc44h[XO|YYm= NISweoVkd22yb4Xu[EB4[XNidHXzeIVlKGmwII\peJJwKG[xcjDpcohq[mm2aX;uJIFkfGm4aYT5JIFo[Wmwc4SgZUB{\W6|aYTpeoUhcHWvYX6gZpJm[XO2IHPhdoNqdm:vYTDsbY5mKGOnbHzzMEBKSzVyIE2gNE4xPiEQvF2u NFvoXlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97MkW4N|UyLz57MkW4N|UyRC:jPh?=
breast carcinoma line cells MXHGeY5kfGmxbjDhd5NigQ>? NGHw[|Zkd22yb4Xu[EB4[XNidHXzeIVlKGmwII\peJJwKG[xcjDpcohq[mm2aX;uJIFkfGm4aYT5JIFo[Wmwc4Sg[I95d3K3YnnjbY4hemW|aYP0ZY51KGOxdX70[ZJx[XK2IH;mJIh2dWGwIHLy[YF{fCClYYLjbY5wdWFibHnu[UBk\WyuczygTWM2OCB;IEKg{txONg>? NFHsZ2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97MkW4N|UyLz57MkW4N|UyRC:jPh?=
MCF-7 MXHGeY5kfGmxbjDhd5NigQ>? Mom1R49ueG:3bnSge4F{KHSnc4Tl[EBnd3JiaYTzJJRwgGmlaYT5JIFo[Wmwc4SgbJVu[W5iYoLlZZN1KGOjbnPldkBk\WyuczjNR2YuPyluIFnDOVAhRSByLkC2JO69VS5? NIXTUIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97NUS4PFIxLz57NUS4PFIxRC:jPh?=
MCF-7/ADR MWXGeY5kfGmxbjDhd5NigQ>? Ml[wR49ueG:3bnSge4F{KHSnc4Tl[EBnd3JiaYTzJJRwgGmlaYT5JIFo[Wmwc4Sg[I95d3K3YnnjbY4hemW|aYP0ZY51KGi3bXHuJIJz\WG|dDDjZY5k\XJiY3XscJMhME2FRj23M2FFWiluIFnDOVAhRSB{IN88UU4> MkTEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTV2OEiyNEc,QTV2OEiyNFww[T5?
L1210 NF;KSIJHfW6ldHnvckBie3OjeR?= MofUTY5pcWKrdH;yfUBi[3Srdnn0fUB4[XNibXXhd5Vz\WRiYXfhbY5{fCCvdYLpcoUhVDF{MUCgcIV2c2WvaXGgZ4VtdHNuIFnDOVAhRSByLkCzJO69VS5? Mom0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTd|M{S4N{c,QTd|M{S4N|ww[T5?
HL60 NXS2W29mTnWwY4Tpc44h[XO|YYm= MWjJckB3cXS{bzDpcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCycn;sbYZmemG2aX;uJI9nKEiONkCgZ4VtdHNuIFnDOVAhRSByLkCwOUDPxE1w M4exfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEe3OVk{Lz5zMki3O|U6OzxxYU6=
HL60R NYrsUYttTnWwY4Tpc44h[XO|YYm= NXTIeIJQUW5idnn0do8hcW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDIUFYxWiClZXzsd{BidG:wZzD3bZRpKDViKIXNLUB3\XKjcHHtbYwtKEmFNUCgQUAxNjB2IN88UU4> NHHOTow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMki3O|U6Oyd-MUK4O|c2QTN:L3G+
HL60R MXHGeY5kfGmxbjDhd5NigQ>? M4DFPGlvKH[rdILvJIlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IIDyc4xq\mW{YYTpc44hd2ZiSFy2NHIh[2WubIOsJGlEPTBiPTCxMlUh|ryPLh?= NHe0coQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMki3O|U6Oyd-MUK4O|c2QTN:L3G+
K562 Mk\iR5l1d3SxeHnjbZR6KGG|c3H5 M4TxW2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KEt3NkKgZ4VtdHNiYomgUXRUNCCLQ{WwJF0hOC5yMUWg{txONg>? NFHt[4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkS1NVA2QSd-MU[0OVExPTl:L3G+
P388 NITYXopCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NHvxO2xCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JHA{QDhiY3XscJMh[nliRVzJV2EtKEmFNUCgQUAxNjB{NTFOwG0v NVrLe4dERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexOVQ2ODVpPkG3NVU1PTB3PD;hQi=>
P388 M33nSmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHHkdolidXmlaX6gdoV{cXO2YX70JHA{QDhiY3XscJMhcW5icILld4Vv[2Vib3[gOUB2VSC4ZYLhdIFucWxiYomgSWxKW0FuIFnDOVAhRSByLkC0JO69VS5? Ml20QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUS1NFUoRjF5MUW0OVA2RC:jPh?=
MDA435/LCC6 M2W1NmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MlP0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDNSGE1OzVxTFPDOkBk\WyuczDifUBGVEmVQTygTWM2OCB;IECuNFc6KM7:TT6= NIK2XpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G1OFUxPSd-MUexOVQ2ODV:L3G+
P388 MVTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NW[xc3VLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBi\HKrYX35Z4lvKHKnc3nzeIFvfCCSM{i4JINmdGy|IHnuJJBz\XOnbnPlJI9nKDVidV2gNUwyOy2kaYPbOEcuMCh3LEet[IlpgWS{b4j5LU01UC2laILvcYVvNTRvb36tNk16dCmyaHXufYxeNTFuNDy3MFExNDF|LYDlcpRid3ijdILp[IVk[W6nIHL5JGVNUVODLDDJR|UxKD1iMD6xNFYh|ryPLh?= M2DrclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUW0OVA2Lz5zN{G1OFUxPTxxYU6=
MDA435/LCC6 MnrKRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M3HI[GFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcZVtfGmmcoXnJJJme2m|dHHueEBOTEF2M{WvUGNEPiClZXzsd{BqdiCycnXz[Y5k\SCxZjC1JJVOKDFuMUOtZol{YzRpLTioOUw4NWSraInkdo95gSlvNFitZ4hzd22nbj20MY9vNTJveXypdIhmdnmuXT2xMFQtPyxzMDyxN{1x\W62YX;4ZZRzcWSnY3Hu[UBjgSCHTFnTRUwhUUN3MDC9JFAvOTJ7IN88UU4> MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3NEWwOUc,OTdzNUS1NFU9N2F-
MDA435/LCC6 M3jBcmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M{K4emFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcZVtfGmmcoXnJJJme2m|dHHueEBOTEF2M{WvUGNEPiClZXzsd{BjgSCHTFnTRUwhUUN3MDC9JFAvQTd5IN88UU4> MorjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUS1NFUoRjF5MUW0OVA2RC:jPh?=
P388 NXq4ToU5SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NYnnTnVTSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBi\HKrYX35Z4lvKHKnc3nzeIFvfCCSM{i4JINmdGy|IHL5JGVNUVODLDDJR|UxKD1iMj6xNVEh|ryPLh?= Mk\GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUS1NFUoRjF5MUW0OVA2RC:jPh?=
A2780 NGT5S5JEgXSxdH;4bYNqfHliYYPzZZk> NIDEWnk4OiCqcoO= MVPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4yOTd2OTFOwG0v Mkn5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OUCwPVQoRjF5OEmwNFk1RC:jPh?=
A2780/ADR MV;DfZRwfG:6aXPpeJkh[XO|YYm= NVrBN3FyPzJiaILz MlfZR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBxQVTSJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzOD62NlA6KM7:TT6= MojGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OUCwPVQoRjF5OEmwNFk1RC:jPh?=
NIH/3T3 MkjBR5l1d3SxeHnjbZR6KGG|c3H5 MlnCO|IhcHK| M{XYOmlvfHKrboPpZ{BkgXSxdH;4bYNqfHliYXfhbY5{fCC5aXzkJJR6eGVibX;1d4UhVkmKL{PUN{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6wOFQh|ryPLh?= NFPJSWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEm1OFA1OCd-MUi5OVQxPDB:L3G+
NIH/3T3 M1v4OWN6fG:2b4jpZ4l1gSCjc4PhfS=> NEf0clE4OiCqcoO= MYHJcpRzcW6|aXOgZ5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUmlJNzOWMzDj[YxteyCxdnXy[ZhxemW|c3nu[{BOTFJzIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkCxJO69VS5? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl3NEC0NEc,OTh7NUSwOFA9N2F-
KB MYDDfZRwfG:6aXPpeJkh[XO|YYm= MUS0PEBpenN? NX3jSng1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvPjRizszNMi=> NX\WSmlRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzOlMyPDJpPkKwN|Y{OTR{PD;hQi=>
KB-C2 NVricXp{S3m2b4TvfIlkcXS7IHHzd4F6 M1LNO|Q5KGi{cx?= NInNOI5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBufWy2aXTyeYcuemW|aYP0ZY51KEuELVOyJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzNj6yJO69VS5? M1Pye|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{[zNVQzLz5{MEO2N|E1OjxxYU6=
HT-29 M3\0TGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGj3[GczPCCqcoO= MljvR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VWyCjc4PhfUwhUUN3MDC9JFAvPCEQvF2u Mn;IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOUSwOFkoRjJzMEm0NFQ6RC:jPh?=
HepG2 M2TxPWN6fG:2b4jpZ4l1gSCjc4PhfS=> MmDHNlQhcHK| NEHJZWlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwJF0hOC55IN88UU4> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB7NEC0PUc,OjFyOUSwOFk9N2F-
NIH/3T3 NUP1fFVnS3m2b4TvfIlkcXS7IHHzd4F6 MkHEN{Bl[Xm| NI\KZ2REgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBPUUhxM2SzJINmdGy|IHHmeIVzKDNiZHH5d{BjgSCPVGOgZZN{[XluIFnDOVAhRSBzLkig{txONg>? MoXuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOUSwOFkoRjJzMEm0NFQ6RC:jPh?=
Vero MWTDfZRwfG:6aXPpeJkh[XO|YYm= MmfTN{Bl[Xm| MnvoR5l1d3SxeHnjbZR6KGGpYXnud5Qh[W[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDh[pRmeiB|IHThfZMh[nliTWTTJIF{e2G7LDDJR|UxKD1iMj61JO69VS5? MknWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOUSwOFkoRjJzMEm0NFQ6RC:jPh?=
MCF7 MlTYR5l1d3SxeHnjbZR6KGG|c3H5 MoDPNlQhcHK| M1;jZ2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VWyCjc4PhfUwhUUN3MDC9JFUvPSEQvF2u MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB7NEC0PUc,OjFyOUSwOFk9N2F-
CRL7761 NEXhN29EgXSxdH;4bYNqfHliYYPzZZk> NF3FNIM{KGSjeYO= MkjVR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR3JNPzd4MTDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSVIHHzd4F6NCCLQ{WwJF0hOTBwNTFOwG0v M4SyT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEm0NFQ6Lz5{MUC5OFA1QTxxYU6=
NCI-H460 NHLIc5RCdnSrY3HuZ4VzKGG|c3H5 NEHxO3RCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUmNKNUh2NkCgZ4VtdHNuIFnDOVAhRSByLkO4JO69VS5? NXzGZZgxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1PVkxODBpPkKxOVk6ODByPD;hQi=>
SF268 MWXBcpRq[2GwY3XyJIF{e2G7 MVPBcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1[yOlgh[2WubIOsJGlEPTBiPTCwMlYh|ryPLh?= NHj5NFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUW5PVAxOCd-MkG1PVkxODB:L3G+
MRC5 MoD3RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MUjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2UQ{WgZ4VtdHNuIFnDOVAhRSByLki4JO69VS5? NVH1NFlqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1PVkxODBpPkKxOVk6ODByPD;hQi=>
MCF7 M{OzV2FvfGmlYX7j[ZIh[XO|YYm= NGn4T49CdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{whUUN3MDC9JFUvODNizszNMi=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV7OUCwNEc,OjF3OUmwNFA9N2F-
DU145 NIXLc2VCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NH;iRZI4OiCqcoO= NUXYTIRLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRUKGG|c3H5MEBKSzVyIE2gNE4xQSEQvF2u M{XO[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[wNVY3Lz5{MkK2NFE3PjxxYU6=
SKMES1 MYnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnfUO|IhcHK| NXvJWGpiSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT21GWzFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VWyCjc4PhfUwhUUN3MDC9JFAvOTdizszNMi=> M1LFOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[wNVY3Lz5{MkK2NFE3PjxxYU6=
MCF7 MmX6RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? Mn\kO|IhcHK| MVrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiSVO1NEA:KDBwOUWg{txONg>? NFTyUWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2NFE3Pid-MkKyOlAyPjZ:L3G+
LNCAP NUiyfoJNS3m2b4P0ZZRq[yCjc4PhfS=> NITTO2U1KGSjeYO= M3e5XWN6fG:|dHH0bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMUmNCWCClZXzsd{Bi\nSncjC0JIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4yKM7:TT6= NYTWfmc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0PFA1QTVpPkKyOFgxPDl3PD;hQi=>
HT-29 M{fLfWN6fG:|dHH0bYMh[XO|YYm= M1zIUFQh\GG7cx?= MmnpR5l1d3O2YYTpZ{Bi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IESg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlIh|ryPLh?= MlnyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2OEC0PVUoRjJ{NEiwOFk2RC:jPh?=
MCF7 NFL3SZdEgXSxc4TheIlkKGG|c3H5 Mk\kOEBl[Xm| NFfrbVdEgXSxc4TheIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0JIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE41KM7:TT6= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR6MES5OUc,OjJ2OEC0PVU9N2F-
HepG2 MnP4SpVv[3Srb36gZZN{[Xl? MnvRTGFTXkGUREqgTY5pcWKrdHnvckBw\iCuaY\ldkB{fGGpZTDQcIF{dW:maYXtJIJmemeqZXmgbY5n\WO2aX;uJIlvKEincFeyJINmdGy|LDDJR|UxKD1iMj65PUDPxE1w MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV6NkGyOEc,OjJ3OE[xNlQ9N2F-
THP1 M{nTXGN6fG:2b4jpZ4l1gSCjc4PhfS=> MX63NkBpenN? MlnVR5l1d3SxeHnjbZR6KGGpYXnud5QhWE2DLYP0bY12dGG2ZXSgbJVu[W5iVFjQNUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMk[uPUDPxE1w MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl|OU[5OUc,OjJ7M{m2PVU9N2F-
L5178Y M2f3WGN6fG:2b4jpZ4l1gSCjc4PhfS=> NH;EbHozPCCqcoO= M{fsb2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGw2OTd6WTDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5|IN88UU4> Mn;yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzOUm0O|koRjJ|MUm5OFc6RC:jPh?=
L5178Y M{noNmN6fG:2b4jpZ4l1gSCjc4PhfS=> NFjneJMzPCCqcoO= NX23eoVVS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhVDVzN{jZJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkezJO69VS5? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF7OUS3PUc,OjNzOUm0O|k9N2F-
L5178Y MmPWR5l1d3SxeHnjbZR6KGG|c3H5 M3;HUVI1KGi{cx?= NFT2Z4REgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBNPTF5OGmgZ4VtdHNiZYjwdoV{e2mwZzDwMYdxKGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzNjN{IN88UU4> Ml7uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzOUm0O|koRjJ|MUm5OFc6RC:jPh?=
L5178Y MkHPR5l1d3SxeHnjbZR6KGG|c3H5 MXqyOEBpenN? NF7FU4JEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBNPTF5OGmgZ4VtdHNiZYjwdoV{e2mwZzDwMYdxKGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUA4Njd|IN88UU4> M3TifFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUm5OFc6Lz5{M{G5PVQ4QTxxYU6=
DA-3 M4r3N2N6fG:2b4jpZ4l1gSCjc4PhfS=> MWSyOEBpenN? NGXrUHlEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBFSS1|IHPlcIx{KGGodHXyJFI1KGi{czDifUBZXFRiYYPzZZktKEmFNUCgQUAyNjlizszNMi=> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyME[2PEc,OjR7MEC2Olg9N2F-
SKOV3 NGDuNGpEgXSxdH;4bYNqfHliYYPzZZk> NFjRSVIzPCCqcoO= M4HDfGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMV1Z|IHPlcIx{KGGodHXyJFI1KGi{czDifUBZXFRiYYPzZZktKEmFNUCgQUAzOC54IN88UU4> M1ryNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwOlY5Lz5{NEmwNFY3QDxxYU6=
ES2 NVvWU296S3m2b4TvfIlkcXS7IHHzd4F6 M1f3RlI1KGi{cx?= NFz3Zo9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBGWzJiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JHhVXCCjc4PhfUwhUUN3MDC9JFI2NjVizszNMi=> NUfkOnpKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA3PjhpPkK0PVAxPjZ6PD;hQi=>
MCF7 Moj4R5l1d3SxeHnjbZR6KGG|c3H5 NELkPYEzPCCqcoO= NXSyOYlJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkAzPCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwJF0hOzNwOTFOwG0v NI\0WWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFY3QCd-MkS5NFA3Pjh:L3G+
MRC5 M{jGc2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NUH3epVESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNVmM2KGOnbHzzJIJ6KFiWVDDhd5NigSxiSVO1NEA:KDBwMEe4JO69VS5? M3XyNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NE[3NlkyLz5{NUS2O|I6OTxxYU6=
A549 NEnsXZBCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NHPVZXVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZpkhYFSWIHHzd4F6NCCLQ{WwJF0hOC5yODFOwG0v M2W2SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NE[3NlkyLz5{NUS2O|I6OTxxYU6=
MDA-MB-231 MnvsRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NIrvfHNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYomgXHRVKGG|c3H5MEBKSzVyIE2gNE4xQTFizszNMi=> NYX4cmJXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlczQTFpPkK1OFY4OjlzPD;hQi=>
H1299 MVnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M4fVfWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSEGyPVkh[2WubIOgZpkhYFSWIHHzd4F6NCCLQ{WwJF0hOC5yOU[g{txONg>? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4N{K5NUc,OjV2NkeyPVE9N2F-
MCF7 M4izdWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NYPwVpl5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIJ6KFiWVDDhd5NigSxiSVO1NEA:KDBwNkWg{txONg>? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4N{K5NUc,OjV2NkeyPVE9N2F-
HEK293 MVjDfZRwfG:6aXPpeJkh[XO|YYm= NX:w[IhpOjRiaILz Mn\6R5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCDbHHtZZIhSmy3ZTDhd5NigSxiSVO1NEA:KDF{LkW1JO69VS5? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl|N{iyPEc,OjZ7M{e4Nlg9N2F-
MCF7/ADR M4jrO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUK0PEBpenN? MUHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNR2Y4N0GGUjDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hPy5zMjFOwG0v MkLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{NE[wOFEoRjJ6MkS2NFQyRC:jPh?=
HCT16 NYXMRmFGS3m2b4TvfIlkcXS7IHHzd4F6 NIW2Nm84OiCqcoO= MYnDfZRwfG:6aXPpeJkhcW5iaIXtZY4hUEOWMU[gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iClZXzsJIdzd3e2aDDpcoN2[mG2ZXSg[o9zKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkKxJO69VS5? NILqfnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyN|M5Oid-Mki5NlM{QDJ:L3G+
A549 MlLQR5l1d3SxeHnjbZR6KGG|c3H5 NUK5NVVOPzJiaILz MX\DfZRwfG:6aXPpeJkhcW5iaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGOnbHyg[5Jwf3SqIHnuZ5Vj[XSnZDDmc5IhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwNUGg{txONg>? M3;Z[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUKzN|gzLz5{OEmyN|M5OjxxYU6=
MCF7 NXXtXoJES3m2b4TvfIlkcXS7IHHzd4F6 NX7nUlVJPzJiaILz MmC2R5l1d3SxeHnjbZR6KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iClZXzsJIdzd3e2aDDpcoN2[mG2ZXSg[o9zKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkS0JO69VS5? NGXBSms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyN|M5Oid-Mki5NlM{QDJ:L3G+
RD NITkV5hEgXSxdH;4bYNqfHliYYPzZZk> M4OwVlczKGi{cx?= MVnDfZRwfG:6aXPpeJkhcW5iaIXtZY4hWkRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzNjR3IN88UU4> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl{M{O4Nkc,Ojh7MkOzPFI9N2F-
HEK293 M4TLS2N6fG:2b4jpZ4l1gSCjc4PhfS=> MVy3NkBpenN? NG\KNlFEgXSxdH;4bYNqfHliaX6gbJVu[W5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCpcn;3eIghcW6ldXLheIVlKG[xcjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNVEvOTdizszNMi=> NX[0UW1WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5NlM{QDJpPkK4PVI{Ozh{PD;hQi=>
CCRF-CEM M3vpN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYrwOJh[UW5idnn0do8h\3Kxd4ToJIlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBES1KILVPFUUApcHWvYX6gcJlueGixYnzhd5Rq[yCuZYXr[Y1q[yliY3XscJMtKEmGNUCgQUAxNjB2IN88UU4> NHLIcpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi94OUWxNFQ6Lz54OUWxNFQ6RC:jPh?=
CCRF-CEM NIPyWItHfW6ldHnvckBie3OjeR?= Mn7kTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGNEWkZvQ1XNLIh2dWGwIHzleYtmdWmlIHz5cZBpd2KuYYP0bYMqKGOnbHzzJIlvKGO3bIT1doUtKEmGNUCgQUAxNjB2IN88UU4> NIm3XoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi95MEW3OFI3Lz55MEW3OFI3RC:jPh?=
Sf9 NIXsb5NHfW6ldHnvckBie3OjeR?= NGTHWm9VWF:WUlHOV3BQWlSHUkqgRXRRKGi7ZILvcJl{cXNiaX6gcYVu[nKjbnWgeoV{cWOuZTDmdo9uKEKFUmCt[ZhxemW|c3nu[{BU\jliY3XscJMtKEujY4SgQUAzNjVizszNMi=> M1TPZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzNEO3N|gxLz5zMUSzO|M5ODxxYU6=
GLC4/ADR M1O3VmZ2dmO2aX;uJIF{e2G7 MYfUVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBFVlBvU1egeZB1[WunIHnuJI1mdWK{YX7lJJZme2mlbHXzJIZzd21iR1zDOE9CTFJiY3XscJMtKEurIE2gNE46PSEQvF2u MoXMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTF6OEe5Okc,QTF6OEe5Olww[T5?
SR3A NESxdI9HfW6ldHnvckBie3OjeR?= M2\CZ3RRZ1SUQV7TVG9TXEWUOjDpcohq[mm2aX;uJI9nKEyWQ{SgeZB1[WunIHnuJI1mdWK{YX7lJJZme2mlbHXzJIZzd21iU2KzRUBk\WyuczygT4khRSByLkC3JO69VS5? NIDCTIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97NkS3O|g{Lz57NkS3O|g{RC:jPh?=
Caco-2 MXjGeY5kfGmxbjDhd5NigQ>? MoHRWHBgXFKDTmPQU3JVTVJ8IHnubIljcXSrb36gc4YhXGG6b3ygeJJidnOncHn0bIVtcWGuIITyZY5{eG:{dDCoZoF{[WxidH:gZZBq[2GuKTDpckBE[WOxLUKgZ4VtdHNuIFvpJF0hOi53IN88UU4> MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTRyNUK4O{c,OTF2MEWyPFc9N2F-
MDCK NXLIZ4lJTnWwY4Tpc44h[XO|YYm= NV\wc4R2XFChVGLBUnNRV1KWRWK6JIlvcGmkaYTpc44hd2ZiVnnuZoxie3SrbnWgeJJidnOncHn0bIVtcWGuIITyZY5{eG:{dDCoZoF{[WxidH:gZZBq[2GuKTDpckBOWlB{LXX4dJJme3OrbnegUWREUyClZXzsd{whU2liPTC0PU41KM7:TT6= NILZd4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkGzOFk1Pid-MUKxN|Q6PDZ:L3G+
GLC4/ADR M3PEeWZ2dmO2aX;uJIF{e2G7 NYPwWZRMXFChVGLBUnNRV1KWRWK6JIVn\my3eDDpckBIVEN2L1HEVkBk\WyuczygT40hRSBzLkSg{txONg>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek84QTR3NEC2K|44QTR3NEC2QE9iRg>?
BTI-TN5B1-4 NHzRXHhHfW6ldHnvckBie3OjeR?= NILjdZZVWF:WUlHOV3BQWlSHUkqgRXRRKGi7ZILvcJl{cXNiaX6gcYVu[nKjbnWg[pJi[3Srb36g[pJwdSCKaXfoJGZqfmViKFLUTU1VVjWEMT20LUBk\WyuczygT40hRSBzLke0JO69VS5? MlfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5OEW2PFQoRjFzN{i1Olg1RC:jPh?=
A549 NIfsPHlEgXSxdH;4bYNqfHliYYPzZZk> NEXaOVE4OiCqcoO= NEjWdpZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFczKGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDMR|UxKD1iMD6zOUDPxE1w MmPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7NkiyOVgoRjF7OU[4NlU5RC:jPh?=
MDA-MB-231 MYDDfZRwfG:6aXPpeJkh[XO|YYm= NIfaZoE4OiCqcoO= NYrvWIROS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiTFO1NEA:KDBwOE[g{txONg>? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4OEK1PEc,OTl7NkiyOVg9N2F-
CEM NYrqUG9sS3m2b4TvfIlkcXS7IHHzd4F6 NV\kRXNrPzJiaILz MUDDfZRwfG:6aXPpeJkh[WejaX7zeEBETU1iY3XscJMh[W[2ZYKgO|IhcHK|LDDUR3M2OCB;IECuNFYzKM7:TT6= NIHnS3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[wPFM6Pid-MUe2NFg{QTZ:L3G+
MCF7 Mn\6R5l1d3SxeHnjbZR6KGG|c3H5 NG\Hd|I4OiCqcoO= MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czygWGNUPTBiPTCwMlE1KM7:TT6= M1\XUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkC4N|k3Lz5zN{[wPFM6PjxxYU6=
K562 NIXiT2xEgXSxdH;4bYNqfHliYYPzZZk> MVu3NkBpenN? MmD2R5l1d3SxeHnjbZR6KGGpYXnud5QhUzV4MjDj[YxteyCjZoTldkA4OiCqcoOsJHREWzVyIE2gNE4zOyEQvF2u M{fKZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkC4N|k3Lz5zN{[wPFM6PjxxYU6=
HOS NES1PFBEgXSxdH;4bYNqfHliYYPzZZk> NWiwfWZ[PzJiaILz MmfaR5l1d3SxeHnjbZR6KGGpYXnud5QhUE:VIHPlcIx{KGGodHXyJFczKGi{czygWGNUPTBiPTCwMlM{KM7:TT6= NVq1S4JXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFg{QTZpPkG3OlA5Ozl4PD;hQi=>
G361 MmXrR5l1d3SxeHnjbZR6KGG|c3H5 M{nHWVczKGi{cx?= NEHybpJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBIOzZzIHPlcIx{KGGodHXyJFczKGi{czygWGNUPTBiPTCwMlY4KM7:TT6= Ml;kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEizPVYoRjF5NkC4N|k3RC:jPh?=
HeLa NXL0eGtOS3m2b4TvfIlkcXS7IHHzd4F6 NVS4Oo1sPzJiaILz NUXlTWJSS3m2b4TvfIlkcXS7IHHnZYlve3RiSHXMZUBk\WyuczDh[pRmeiB5MjDodpMtKFSFU{WwJF0hOC54NzFOwG0v NUHEeYJIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFg{QTZpPkG3OlA5Ozl4PD;hQi=>
MDA-MB-231 NGTJcmpEgXSxdH;4bYNqfHliYYPzZZk> Mlv2O|IhcHK| Mm\TR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB5MjDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhXEeLIE2gNE4yOiEQvF2u Ml\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7NkiyOVgoRjF7OU[4NlU5RC:jPh?=
A549 MoHMR5l1d3SxeHnjbZR6KGG|c3H5 MXO3NkBpenN? NUnLfopkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygWGdKKD1iMD6xO{DPxE1w M2P0[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OU[4NlU5Lz5zOUm2PFI2QDxxYU6=
HT-29 MlvBR5l1d3SxeHnjbZR6KGG|c3H5 NXXseXNLPzJiaILz NFfBXmVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygWGdKKD1iMD6zN{DPxE1w MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4OEK1PEc,OTl7NkiyOVg9N2F-
K562 M{LJWGZ2dmO2aX;uJIF{e2G7 NI\5e|RKdmO{ZXHz[UBqdiC2aHWgcIV3\Wxib3[g[JJ2\yC3cIThb4UhcW5iRH;4MZJme2m|dHHueEBMPTZ{IHPlcIx{KGmwIIDy[ZNmdmOnIH;mJIN6[2yxc4Dvdolv\SCxdnXyJIl1eyCjYoPlcoNm M1\rUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEWxNFU6Lz5zNkS1NVA2QTxxYU6=
K562 M4TYSWZ2dmO2aX;uJIF{e2G7 MVzJcoNz\WG|ZTDpckB1cGVibHX2[Ywhd2ZiZIL1[{Bm\m[udYigbY4hTG:6LYLld4l{fGGwdDDLOVYzKGOnbHzzJIlvKHC{ZYPlcoNmKG:oIHP5Z4xwe3Cxcnnu[UBwfmW{IHn0d{Bi[nOnbnPl NEfDZVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkS1NVA2QSd-MU[0OVExPTl:L3G+
KB MUDBdI9xfG:|aYOgZZN{[Xl? Mm\oNUB2VQ>? MWmyOEBpenN? MmDHTY5lfWO2aX;uJI9nKGGyb4D0c5Rq[yCjY4Tpeol1gSCrbjDoeY1idiCNQjDj[YxteyCjdDCxJJVOKGGodHXyJFI1KGi{cx?= M2rmTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkiwNlc2Lz5zOE[4NFI4PTxxYU6=
DU145 NWXSSZNZTnWwY4Tpc44h[XO|YYm= MoDnPVAhdk1? MUK3NkBpenN? NYS0SoZoUW6qaXLpeIlwdiCxZjD0c5BwcXOxbXXyZZNmKDJiaX6gbJVu[W5iRGWxOFUh[2WubIOgZZN{\XO|ZXSgZZMhe3SjYnnsbZpifGmxbjDv[kBmdnq7bXWtSG5CKGOuZXH2ZYdmKGOxbYDs[Zgh[XRiOUCgcm0h[W[2ZYKgO|IhcHK|IHL5JIludXWwb3Lsc5Qh[W6jbInzbZM> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4MEG2Okc,OjJ{NkCxOlY9N2F-
DU145 M3fodGZ2dmO2aX;uJIF{e2G7 NX\oUGUzQTBibl2= M4roN|MxKG2rboO= NXuwfIt5UW6qaXLpeIlwdiCxZjD0c5BwcXOxbXXyZZNmKDJiaX6gbJVu[W5iRGWxOFUh[2WubIOgZZN{\XO|ZXSgZZMhe3SjYnnsbZpifGmxbjDv[kBmdnq7bXWtSG5CKGOuZXH2ZYdmKGOxbYDs[Zgh[XRiOUCgcm0h[W[2ZYKgN|AhdWmwczDifUBqdW23bn;icI91KGGwYXz5d4l{ MkHKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkCxOlYoRjJ{Mk[wNVY3RC:jPh?=
DU145 NULBPI55TnWwY4Tpc44h[XO|YYm= MWq1NFAhdk1? MlfaOEBpenN? MoW0TY5pcWKrdHnvckBw\iCKRFHDOk1u\WSrYYTl[EB1fWK3bHnuJIFk\XS7bHH0bY9vKGmwIHj1cYFvKESXMUS1JINmdGy|IHH0JFUxOCCwTTDh[pRmeiB2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4MEG2Okc,OjJ{NkCxOlY9N2F-
DU145 MlTKSpVv[3Srb36gZZN{[Xl? NVTRc45ZOC5zIITvJFAvPSC3TR?= NVm0No9GOjRiaILz MmfFTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGRWOTR3IHPlcIx{KGG|c3Xzd4VlKGG|IHjpd5RwdmViSEOgbJlx\XKjY3X0fYxifGmxbjDheEAxNjFidH:gNE42KHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4MEG2Okc,OjJ{NkCxOlY9N2F-
DA-3 M1r1cGN6fG:2b4jpZ4l1gSCjc4PhfS=> MXmyNEB2VQ>? MmnROlAhdWmwcx?= MXPDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDERU0{KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjdDCyNEB2VSCjZoTldkA3OCCvaX7zJIJ6KFiWVDDhd5NigQ>? Mk\MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC2OlgoRjJ2OUCwOlY5RC:jPh?=
TC32 MYPxTHRUKGG|c3H5 NUKyPIhieUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGTDN|Ih[2WubIO= NXrBfZd4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
DAOY MWXxTHRUKGG|c3H5 NVjyfVFieUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO= NFTl[nM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
A673 MlnodWhVWyCjc4PhfS=> NELE[FVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucx?= MnuzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC MXTxTHRUKGG|c3H5 MlzUdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= MkfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 NIXBO5hyUFSVIHHzd4F6 NIHHPWZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStN|ch[2WubIO= M{SxUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1 M1SwSJFJXFNiYYPzZZk> NVf5SINkeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM> M1PaPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
U-2 OS MWTxTHRUKGG|c3H5 M2TGdpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCXLUKgU3Mh[2WubIO= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Saos-2 MkOydWhVWyCjc4PhfS=> MlPkdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-SH NXTXOVZpeUiWUzDhd5NigQ>? NFnLb4lyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=> NYPoXWFtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 MWrxTHRUKGG|c3H5 MVXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| NIjuOHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 M4K2UJFJXFNiYYPzZZk> NF3NfolyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO= MmnoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh18 M2WxU5FJXFNiYYPzZZk> MlzVdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKqMUigZ4VtdHN? NVPjVmQ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
OHS-50 MlLzdWhVWyCjc4PhfS=> M3n6eZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCRSGOtOVAh[2WubIO= NXPmc4pSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD NY\BWXlveUiWUzDhd5NigQ>? M3TZd5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>? M3PUOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R) MUHxTHRUKGG|c3H5 M3XqdZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucx?= M3W2VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
fibroblast cells NHj0XlZyUFSVIHHzd4F6 NEG2[VJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiY3;ueJJwdCCKaDD3bYxlKHS7cHWg[oljem:kbHHzeEBk\Wyucx?= NWL0[4NVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh41 MkTKdWhVWyCjc4PhfS=> NFX0PJNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUni0NUBk\Wyucx?= NULHVnZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
P388 MV\BcpRqfHWvb4KgZZN{[Xl? NXe1TWZuOC5{NTD0c{AyOCCvZz;r[y=> MYHBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCSM{i4JINmdGy|IHHscI9oemGodHXkJIlvKG2xdYPlJIF{e2W|c3XkJIF{KHS3bX;yJIdzd3e2aDDpcohq[mm2aX;uJIF1KDBwMkWgeI8hOTBibXevb4c> Moj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ3NU[2NUc,OTJ3NU[2NVww[T5?
Assay
Methods Test Index PMID
Western blot p-Chk1 / Chk1 / p-ATR / ATR 26727128
Immunofluorescence p-Chk1 26727128
Growth inhibition assay Cell viability 30745844

Protocol (from reference)

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.

30 mg/mL

Chemical Information

Molecular Weight 563.98
Formula

C27H29NO10 . HCl

CAS No. 23541-50-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1C(C(CC(O1)OC2CC(CC3=C2C(=C4C(=C3O)C(=O)C5=C(C4=O)C(=CC=C5)OC)O)(C(=O)C)O)N)O.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04562792 Recruiting Drug: Daunorubicin Relapsed Pediatric ALL|Relapsed Pediatric AML|Refractory Acute Myeloid Leukemia|Refractory Acute Lymphoblastic Leukemia Children''s Mercy Hospital Kansas City May 8 2020 Phase 2
NCT03878927 Recruiting Drug: CPX-351|Drug: Gemtuzumab Ozogamicin Acute Myeloid Leukemia Weill Medical College of Cornell University|Jazz Pharmaceuticals|Pfizer August 23 2019 Phase 1
NCT03896269 Unknown status Drug: Liposome-encapsulated Daunorubicin-Cytarabine Blasts 10-19 Percent of Bone Marrow Nucleated Cells|Blasts More Than 5 Percent of Bone Marrow Nucleated Cells|High Risk Chronic Myelomonocytic Leukemia|Recurrent Chronic Myelomonocytic Leukemia|Recurrent High Risk Myelodysplastic Syndrome|Refractory Chronic Myelomonocytic Leukemia|Refractory High Risk Myelodysplastic Syndrome M.D. Anderson Cancer Center|National Cancer Institute (NCI) May 14 2019 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Daunorubicin (RP 13057) HCl | Daunorubicin (RP 13057) HCl supplier | purchase Daunorubicin (RP 13057) HCl | Daunorubicin (RP 13057) HCl cost | Daunorubicin (RP 13057) HCl manufacturer | order Daunorubicin (RP 13057) HCl | Daunorubicin (RP 13057) HCl distributor