Daunorubicin HCl
Catalog No.S3035 Synonyms: Daunomycin HCl
Molecular Weight(MW): 563.98
Daunorubicin HCl inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay.
3 Customer Reviews
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A) MV411 or B) Kasumi 1 cells were incubated with YM155 (0.25 x IC50) in the presence or absence of i) 0.5 x IC50 or ii) 1 x IC50 of Daunorubicin or iii) 0.5 x IC50 or iv) 1 x IC50 of Cytarabine for 72h. Following 72h treatment, cells were subject to a resazurin reduction assay. Percentages were normalised to DMSO controls. Column, mean of 3 independent experiments; Bars, SEM. *p<0.05, **p<0.01, ***p<0.005. Students t-test. Similar trends were observed with 0.5 x and 1 x IC50 of YM155 in combination with Daunorubicin or Cytarabine (data not shown).
Leuk Res, 2015, 39(4):435-44.. Daunorubicin HCl purchased from Selleck.
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IL-17A promotes resistance to daunorubicin. Nalm-6 cells and patient B-ALL cells isolated from 4 B-ALL patients were cultured for 24 h in complete medium with or without IL-17A at 50 ng/ml and subsequently supplemented with daunorubicin at 100, 200, or 400 nM as indicated for 24 h. The cell viability was determined using CCK-8 assay, and apoptosis was determined using Annexin V staining. IL-17A promoted the proliferation and reduced apoptosis of Nalm-6 cells (a, c) and patient B-ALL cells (b, d). Statistical data representing at least 3 independent experiments were shown
J Transl Med, 2016, 14(1):132. Daunorubicin HCl purchased from Selleck.
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LKB1 downregulation increased the sensitivity of KG1a cells to chemotherapy using cell viability assays. The viability of LKB1 knockdown cells was decreased more significantly compared with control cells after treatment with daunorubicin. *P<0.01, vs LKB1-KD; **P<0.001, vs LKB1-KD.
Mol Cell Biochem, 2017, 434(1-2):25-32. Daunorubicin HCl purchased from Selleck.
Purity & Quality Control
Choose Selective Topoisomerase Inhibitors
Biological Activity
| Description | Daunorubicin HCl inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| In vitro |
At drug concentrations that reflect the peak plasma concentration after Daunorubicin administration, the primary mechanism is likely to be through interaction with topoisomerase II, which may be a primary triggering event for growth arrest and/or cell killing through a signalling pathway leading to apoptosis, at least in leukemic cells and thymocytes. The quinone structure permits daunorubicin to act as electron acceptors in reactions mediated by oxoreductive enzymes including cytochrome P450 reductase, NADH dehydrogenase, and xanthine oxidase. At Daunorubicin concentrations exceeding approximately 2–4 μM, free radical mediated toxicity and DNA cross-linking may become evident. Daunorubicin inhibits both DNA and RNA syntheses in HeLa cells over a concentration range of 0.2 through 2 μM. Daunorubicin inhibits both DNA syntheses in Ehrlich ascites tumor cells over a concentration range of 4 μM. Daunorubic triggers apoptosis at concentrations of 0.5 and 1 μM in either HL-60 or U-937 human leukemic cells. [1] Daunorubicin stimulates ceramide elevation and apoptosis in P388 and U937 cells through de novo synthesis via activation of the enzyme ceramide synthase. [2] Daunorubicin dose-dependently increases the phosphatidylserine exposure and consequent procoagulant activity of human umbilical vein endothelial cells. Daunorubicin (0.2 mM) significantly enhances the release of endothelial microparticles which are highly procoagulant in human umbilical vein endothelial cells. [3] |
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| Cell Data |
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Protocol
Solubility (25°C)
| In vitro | DMSO | 100 mg/mL (177.31 mM) |
|---|---|---|
| Water | 100 mg/mL (177.31 mM) | |
| Ethanol | Insoluble | |
| In vivo | Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): Saline For best results, use promptly after mixing. |
30 mg/mL |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 563.98 |
|---|---|
| Formula | C27H29NO10 . HCl |
| CAS No. | 23541-50-6 |
| Storage | powder |
| in solvent | |
| Synonyms | Daunomycin HCl |
Bio Calculators
Molarity Calculator
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Molarity Calculator
Clinical Trial Information
| NCT Number | Recruitment | Conditions | Sponsor/Collaborators | Start Date | Phases |
|---|---|---|---|---|---|
| NCT03836209 | Not yet recruiting | Acute Myeloid Leukemia | PrECOG LLC.|Astellas Pharma Inc | July 2019 | Phase 2 |
| NCT03836209 | Not yet recruiting | Acute Myeloid Leukemia | PrECOG LLC.|Astellas Pharma Inc | July 2019 | Phase 2 |
| NCT03825796 | Not yet recruiting | Blasts 5 Percent or More of Bone Marrow Nucleated Cells|IDH2 Gene Mutation|Recurrent Acute Myeloid Leukemia | Jonsson Comprehensive Cancer Center|National Cancer Institute (NCI) | March 1 2019 | Phase 2 |
| NCT03860844 | Recruiting | Acute Lymphoblastic Leukemia|Acute Myeloid Leukemia | Sanofi | March 11 2019 | Phase 2 |
| NCT03860844 | Recruiting | Acute Lymphoblastic Leukemia|Acute Myeloid Leukemia | Sanofi | March 11 2019 | Phase 2 |
| NCT03850535 | Recruiting | Acute Myeloid Leukemia | Hoffmann-La Roche | March 16 2019 | Phase 1|Phase 2 |
Tech Support
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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