Daunorubicin HCl

For research use only.

Catalog No.S3035 Synonyms: Daunomycin HCl

30 publications

Daunorubicin HCl Chemical Structure

Molecular Weight(MW): 563.98

Daunorubicin HCl inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay. Daunorubicin is a topoisomerase II inhibitor that induces apoptosis.

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Selleck's Daunorubicin HCl has been cited by 30 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Daunorubicin HCl inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay. Daunorubicin is a topoisomerase II inhibitor that induces apoptosis.
Targets
DNA synthesis [1]
(Cell-free assay)
20 nM(Ki)
In vitro

At drug concentrations that reflect the peak plasma concentration after Daunorubicin administration, the primary mechanism is likely to be through interaction with topoisomerase II, which may be a primary triggering event for growth arrest and/or cell killing through a signalling pathway leading to apoptosis, at least in leukemic cells and thymocytes. The quinone structure permits daunorubicin to act as electron acceptors in reactions mediated by oxoreductive enzymes including cytochrome P450 reductase, NADH dehydrogenase, and xanthine oxidase. At Daunorubicin concentrations exceeding approximately 2–4 μM, free radical mediated toxicity and DNA cross-linking may become evident. Daunorubicin inhibits both DNA and RNA syntheses in HeLa cells over a concentration range of 0.2 through 2 μM. Daunorubicin inhibits both DNA syntheses in Ehrlich ascites tumor cells over a concentration range of 4 μM. Daunorubic triggers apoptosis at concentrations of 0.5 and 1 μM in either HL-60 or U-937 human leukemic cells. [1] Daunorubicin stimulates ceramide elevation and apoptosis in P388 and U937 cells through de novo synthesis via activation of the enzyme ceramide synthase. [2] Daunorubicin dose-dependently increases the phosphatidylserine exposure and consequent procoagulant activity of human umbilical vein endothelial cells. Daunorubicin (0.2 mM) significantly enhances the release of endothelial microparticles which are highly procoagulant in human umbilical vein endothelial cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
P388 leukemia cell line NXn4d2Y5S3m2b4TvfIlkyqCjc4PhfS=> MWnDc41xd3WwZDD3ZZMh\X[jbIXheIVlKG[xcjDjfZRwfG:6aXPpeJkh[WejaX7zeEBROzh6IHzleYtmdWmjIHPlcIwhdGmwZTygTWM2OD1|IH7N NXf6bWtpOTB2N{myPVc>
HL60 cells NXnafII4TnWwY4Tpc44h[XO|YYm= NYLvO3BTUW5idnn0do8hcW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDIUFYxKGOnbHzzMEBKSzVyPUCuNFA2KM7:TR?= MkO3NVI5Pzd3OUO=
human epidermoid carcinoma cell line KB-3-1 NV;6WWZGS3m2b4TvfIlkyqCjc4PhfS=> MYrJckB3cXS{bzDjfZRwfG:6aXOgZYN1cX[rdImge4F{KGW4YXz1ZZRm\CC3c3nu[{B1cGViaIXtZY4h\XCrZHXycY9q\CClYYLjbY5wdWFiY3XscEBtcW6nIFvCMVMuOSxiSVO1NF04KG6P MlXONVI1PDN5NkO=
human K562 cells MlnPR5l1d3SxeHnjxsBie3OjeR?= MV:3NkBp MmfjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bi\nSncjC3NkBpenNiYomgXHRVKGG|c3H5MEBIUTVyPUeuOEBvVQ>? MWCyNFU6OTZ5OB?=
Dx5 w/PSC cell lines MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3vFfHRp\SClb33wc5Vv\CC5YYOg[ZZidHWjdHXkJIZweiC2aHWg[5Jwf3SqIHnubIljcXSrb36gc4YhTHh3IIevVHNEKGOnbHygcIlv\XNidYPpcochVVSWIHHzd4F6NCCLQ{WwQVE1KG6P NHvSR5UyODZ7OES0PS=>
MDA MB 361(mammary carcinoma cell line) NVfkTY9QS3m2b4TvfIlkyqCjc4PhfS=> MlLnWIhmKGOxbYDveY5lKHejczD0[ZN1\WRiZn;yJIlvfHKjY3XscJVt[XJiY4n0c5RwgGmlaYT5JIFo[Wmwc4SgUWRCKE2EIEO2NUhu[W2vYYL5JINiemOrbn;tZUBk\WyuIHzpcoUqKGW6cILld5NqdmdiTHHjXkAp[mW2YT3nZYxi[3Sxc3nkZZNmMSxiSVO1NF0yPyCwTR?= MUexNFM6PTR7MB?=
human MDA-MB-231 cells MW\DfZRwfG:6aXRCpIF{e2G7 NHrIPXY4OiCq NETCc|NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvODJizszN MnPoNVk6Pjh{NUi=
MES-SA uterine tumor cell lines NYPmZYpOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3fO[HRp\SClb33wc5Vv\CC5YYOg[ZZidHWjdHXkJIZweiC2aHWg[5Jwf3SqIHnubIljcXSrb36gc4YhVUWVLWPBJJV1\XKrbnWgeJVud3JiY3XscEBtcW6nczD1d4lv\yCPVGSgZZN{[XluIFnDOVA:OC5yMkGg{txO MnPrNVA3QTh2NEm=
murine L1210 leukemia cells NI\yfmdHfW6ldHnvckBie3OjeR?= NH\wNYZKdmirYnn0c5J6KGGldHn2bZR6KHejczDt[YF{fXKnZDDh[4FqdnO2IH31dolv\SCOMUKxNEBt\XWtZX3pZUBk\WyuczygTWM2OD1|MDDuUS=> NIq3eFk6PzN|NEiz
HL60 cells MoXCSpVv[3Srb36gZZN{[Xl? MoT5TY4hfmm2cn:gZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcoR2[2ViYYDvdJRwe2m|IHnuJGhNPjBiY3XscJMtKEGFNUC9NE4xPCEQvF2= MYqxNlg4PzV7Mx?=
CCRF-CEM (human lymphoblastic leukemic) cells MlvTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIjKU4pKdiC4aYTyc{Boem:5dHigbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGNEWkZvQ1XNJEhpfW2jbjDsfY1xcG:kbHHzeIlkKGyndXvlcYlkMSClZXzsd{whUUR3ME2wMlA1KM7:TR?= NW[yXVJOPjl3MUC0PS=>
mouse NIH/3T3 cells M2Po[GN6fG:2b4jpZ:Kh[XO|YYm= MVK3NkBp M4f0NGlvfHKrboPpZ{BkgXSxdH;4bYNqfHliYXfhbY5{fCC5aXzkJJR6eGVibX;1d4UhVkmKL{PUN{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMES0JO69VQ>? M1XDS|E5QTV2MESw
human prostate cancer HTB-81 cells NWLhZ2ZFS3m2b4TvfIlkyqCjc4PhfS=> NHq2WoFEgXSxdH;4bYNqfHlidHjheEBk[XW|ZXSgZUA2OCVicnXkeYN1cW:wIHnuJIFje2:{YnHuZ4Uh[XRiNEmwJI5uKGmwIHj1cYFvKHC{b4P0ZZRmKGOjbnPldkBJXEJvOEGgZ4VtdHNidYPpcochVVSVIHHzd4F6NCCHQ{WwQVAvODV6IN88US=> NVX0e2xROTFyMkCyPFU>
human breast carcinoma line cells NWrvSVdmTnWwY4Tpc44h[XO|YYm= MmPIZ49ueG:3bnSge4F{KHSnc4Tl[EBqdiC4aYTyc{Bnd3JiaX7obYJqfGmxbjDhZ5Rqfmm2eTDh[4FqdnO2IHGgd4Vve2m2aY\lJIh2dWGwIHLy[YF{fCClYYLjbY5wdWFibHnu[UBk\WyuczygTWM2OD1yLkC2JO69VQ>? MkXxPVI2QDN3MR?=
MCF-7 cells NUjocVFGS3m2b4TvfIlkyqCjc4PhfS=> NXvkd4w5S2:vcH;1coQhf2G|IITld5Rm\CCob4KgbZR{KHSxeHnjbZR6KGGpYXnud5QhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsd{hOS0ZvNzmsJGlEPTB;MD6wOkDPxE1? MUK5OVQ5QDJy
human HT-29 cells Mo\iR5l1d3SxeHnjxsBie3OjeR?= NWrwR4JMPzJiaB?= NHG4T29EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygS2k2OD1yLkC3JO69VQ>? NYraflM2OTl7NkiyOVg>
human uterine sarcoma cell line MES-SA NGPq[JZEgXSxdH;4bYPDqGG|c3H5 M3Xo[WlvKH[rdILvJIN6fG:2b4jpZ{Bi[3Srdnn0fUB4[XNiZY\hcJVifGWmIIXzbY5oKHSqZTDoeY1idiC3dHXybY5mKHOjcnPvcYEh[2WubDDsbY5mKE2HUz3TRUwhUUN3ME2wMlA4KM7:TR?= M2T3[|EzPDR|N{[z
human MRC5 cells MkLKVJJwdGmoZYLheIlwdiCjc4PhfS=> MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2UQ{WgZ4VtdHNiYomgXHRVKGG|c3H5MEBKSzVyPUCuNFc5KM7:TR?= MYSyOVQ3PzJ7MR?=
MDA435/LCC6 cells NYWzOXljWHKxbHnm[ZJifGmxbjDhd5NigQ>? MUPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IF3ERVQ{PS:OQ1O2JINmdGy|IHL5JGVNUVODLDDJR|UxRTBwMEe5JO69VQ>? M32wPFE4OTV2NUC1
human A549 cells NGfSPFJEgXSxdH;4bYPDqGG|c3H5 NIr1SIREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFczKGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDHTVUxRTBwMEig{txO NFHnWZgyQTl4OEK1PC=>
Jurkat T lymphocytes NVy2ZoFDS3m2b4TvfIlkyqCjc4PhfS=> MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBLfXKtYYSgWEBtgW2yaH;jfZRmeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6wPFI3KM7:TR?= NW\CWJpYOTZ|Nk[2NFI>
human DU145 cells M4HmcHBzd2yrZnXyZZRqd25iYYPzZZk> M4K5RVczKGh? M3e5PGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRGWxOFUh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1yLkC5JO69VQ>? NEK0fXAzOjJ4MEG2Oi=>
human MDA-MB-231 cells M174[nBzd2yrZnXyZZRqd25iYYPzZZk> MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[nliWGTUJIF{e2G7LDDJR|UxRTBwMEmxJO69VQ>? M3yw[FI2PDZ5Mkmx
human H1299 cells NFXMS45Rem:uaX\ldoF1cW:wIHHzd4F6 MWjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEhzMkm5JINmdGy|IHL5JHhVXCCjc4PhfUwhUUN3ME2wMlA6PiEQvF2= MmLQNlU1Pjd{OUG=
human HL60 cells NHmwOFNEgXSxdH;4bYPDqGG|c3H5 MmL6OFghcA>? MmSxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhfHK7cHHuJIJtfWVic4ThbY5qdmdvYnHz[YQhcGWvb3P5eI9u\XS{aXOgZY5idHm|aYOsJGdKPTB;MD6xJO69VQ>? NGH5cmYzPTh5NEOzOS=>
human LNCAP cells MYnDfZRwfG:6aXRCpIF{e2G7 MXG0JIRigXN? M2\JU2N6fG:|dHH0bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMUmNCWCClZXzsd{Bi\nSncjC0JIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNUDPxE1? MXSyNlQ5ODR7NR?=
NCI-H69 cell line NXHSOJJLWHKxbHnm[ZJifGmxbjDhd5NigQ>? NIPi[XVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIRzfWdic3Xud4l1cX[nIF7DTU1JPjliY3XscEBtcW6nIHL5JGFt[W2jcjDCcJVmKGG|c3H5MEBKSzVyPUCuNUDPxE1? MnXCNVczQDZ|OUO=
human A2780 cells NEL3e4xEgXSxdH;4bYPDqGG|c3H5 M1vRdVczKGh? NIHaVplEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvOTF5NEmg{txO MljsNVc5QTByOUS=
human HT-29 cells NYTnR|NGWHKxbHnm[ZJifGmxbjDhd5NigQ>? MVq5OkBp MX;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC5zM{Og{txO MmjZNlc4QDR2OR?=
B16 mouse melanoma cells Mkj2R5l1d3SxeHnjxsBie3OjeR?= NHXxbmxEgXSxdH;4bYNqfHlidHjheEBk[XW|ZXSgZUA2OCVicnXkeYN1cW:wIHnuJIFje2:{YnHuZ4Uh[XRiNEmwJI5uKGmwIFKxOkBud3W|ZTDt[Yxidm:vYTDj[YxteyC3c3nu[{BOXFNiYYPzZZktKEWFNUC9NE4yPiEQvF2= NWG4NmVkOTFyMkCyPFU>
human SKMES1 cells MnHNVJJwdGmoZYLheIlwdiCjc4PhfS=> NVXpcY1sPzJiaB?= MmruRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVS13FV|Eh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1yLkG3JO69VQ>? NXrtS5hKOjJ{NkCxOlY>
human fibroblast (NHF) cells MWnDfZRwfG:6aXRCpIF{e2G7 MY\DfZRwfG:6aXPpeJkhfGijdDDjZZV{\WRiYTC1NEUhemWmdXP0bY9vKGmwIHHid49z[mGwY3WgZZQhPDlyIH7tJIlvKG6xcn3hcEBpfW2jbjDmbYJzd2KuYYP0JEhPUEZrIHPlcIx{KHW|aX7nJG1VWyCjc4PhfUwhTUN3ME2wMlIzKM7:TR?= M1jublEyODJyMki1
mouse L1210 cells Ml;pSpVv[3Srb36gZZN{[Xl? NH\LV4pKdmirYnn0bY9vKG:oIGLORUB{gW62aHXzbZMhcW5ibX;1d4UhVDF{MUCgZ4VtdHNuIFXEOVA:OC5{MzFOwG0> MmDKOlkxQTl7
human NCI-H460 cells M4LwNWZ2dmO2aX;uJIF{e2G7 M2W2XWFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDR4MDDj[YxteyxiSVO1NF0xNjN6IN88US=> MVGyNVU6QTByMB?=
human MCF7 cells NHTUfHFEgXSxdH;4bYPDqGG|c3H5 MVm0JIRigXN? NYPzSWs2S3m2b4P0ZZRq[yCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOEBl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlQh|ryP MY[yNlQ5ODR7NR?=
human SF268 cells MkXFSpVv[3Srb36gZZN{[Xl? MY\BcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1[yOlgh[2WubIOsJGlEPTB;MD62JO69VQ>? NFjXXlIzOTV7OUCwNC=>
human KB cells MlXLR5l1d3SxeHnjxsBie3OjeR?= M2n0T|Q5KGh? MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwNkSg{txO NI\0PHYzODN4M{G0Ni=>
human HepG2 cells NXXHe4M2S3m2b4TvfIlkyqCjc4PhfS=> MkSyNlQhcA>? NYfKdFh[S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0xNjdizszN MVmyNVA6PDB2OR?=
mouse L5178Y cells NGjoO4pEgXSxdH;4bYPDqGG|c3H5 Ml\ZNlQhcA>? NFjIcI5EgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBNPTF5OGmgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjd{IN88US=> Mkj3NlMyQTl2N{m=
human MCF7 cells MXPDfZRwfG:6aXRCpIF{e2G7 MljqO|IhcA>? MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDYWHQh[XO|YYmsJGdKPTB;MD64OkDPxE1? NUPFPHBHOjB3OUG2O|g>
human MRC5 cells NXXnc2FMWHKxbHnm[ZJifGmxbjDhd5NigQ>? M1vkUGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTWLDOUBk\WyuczygTWM2OD1yLki4JO69VQ>? M{\6SVIyPTl7MECw
MDA435/LCC6 cells NVj2bGFiWHKxbHnm[ZJifGmxbjDhd5NigQ>? M2TUR2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcZVtfGmmcoXnJJJme2m|dHHueEBOTEF2M{WvUGNEPiClZXzsd{BjgSCHTFnTRUwhUUN3ME2wMlk4PyEQvF2= M2fHcVE4OTV2NUC1
mouse NIH/3T3 cells NVTYbW14S3m2b4TvfIlkyqCjc4PhfS=> NIjQU3Q4OiCq MmToTY51emmwc3njJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJG5KUC9|VEOgZ4VtdHNib4\ldoV5eHKnc4PpcochVUSUMTDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTFwMEGg{txO M3jte|E5QTV2MESw
human Detroit 551 cells M2LPPWN6fG:2b4jpZ:Kh[XO|YYm= MmfkO|IhcA>? NYWySIxZS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTGW2cn;peEA2PTFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHhVXCCjc4PhfUwhT0l3ME2xMlc4KM7:TR?= NVvtSGRXOjB3OUG2O|g>
mouse DA-3 cells MkfNR5l1d3SxeHnjxsBie3OjeR?= M{\4d|I1KGh? Ml3HR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgSGEuOyClZXzsd{Bi\nSncjCyOEBpenNiYomgXHRVKGG|c3H5MEBKSzVyPUGuPUDPxE1? MV:yOFkxODZ4OB?=
african green monkey Vero cells M4TnemN6fG:2b4jpZ:Kh[XO|YYm= M1HuWlMh\GG7cx?= MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBi\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|IHHmeIVzKDNiZHH5d{BjgSCPVGOgZZN{[XluIFnDOVA:Oi53IN88US=> NVPhZoI1OjFyOUSwOFk>
Dx5 cell lines M4\zRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MW\UbIUh[2:vcH;1coQhf2G|IHX2ZYx2[XSnZDDmc5IhfGinIHfyc5d1cCCrbnjpZol1cW:wIH;mJGR5PSClZXzsJIxqdmW|IIXzbY5oKE2WVDDhd5NigSxiaXO1NF0zKM7:TR?= NX3qNoJbOTB4OUi0OFk>
human HeLa3 cell line NIDEZZhEgXSxdH;4bYPDqGG|c3H5 NGjPfXNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjMzDj[YxtKGyrbnWgZpkhVVSWIHHzd4F6NCCLQ{WwQVIvQTZizszN NWPKZpFNOTZ7NEKwN|c>
mouse L5178Y cells NVjpUI9VS3m2b4TvfIlkyqCjc4PhfS=> MnHiNlQhcA>? MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDMOVE4QFliY3XscJMh\XiycnXzd4lv\yCyLXfwJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Nz63N{DPxE1? MkPYNlMyQTl2N{m=
human CRL7761 cells NUCyRZZXS3m2b4TvfIlkyqCjc4PhfS=> MknsN{Bl[Xm| MnjRR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR3JNPzd4MTDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSVIHHzd4F6NCCLQ{WwQVExNjVizszN MX[yNVA6PDB2OR?=
human A2780/ADR cells NYXVR3lkS3m2b4TvfIlkyqCjc4PhfS=> Mn7tO|IhcA>? NX\NNJhIS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECvRWRTKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUiuOlIxQSEQvF2= M4L1dFE4QDlyMEm0
human SKOV3 cells NFH4VIJEgXSxdH;4bYPDqGG|c3H5 MoG4NlQhcA>? NWrKZWVRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uRVkOgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFiWVDDhd5NigSxiSVO1NF0zOC54IN88US=> MUSyOFkxODZ4OB?=
human ES2 cells NIjXcGFEgXSxdH;4bYPDqGG|c3H5 NXzXUHhpOjRiaB?= NI\aemJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBGWzJiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JHhVXCCjc4PhfUwhUUN3ME2yOU42KM7:TR?= MluzNlQ6ODB4Nki=
CEM cells M1Pzc2N6fG:2b4jpZ:Kh[XO|YYm= MmXKO|IhcA>? NYH3OYdRS3m2b4TvfIlkcXS7IHHnZYlve3RiQ1XNJINmdGy|IHHmeIVzKDd{IHjydy=> NV7uZ24xOTd4MEizPVY>
HOS cells NHfTZW1EgXSxdH;4bYPDqGG|c3H5 NIXNW3o4OiCq NXvD[5ZYS3m2b4TvfIlkcXS7IHHnZYlve3RiSF;TJINmdGy|IHHmeIVzKDd{IHjydy=> NVrrUZZCOTd4MEizPVY>
mouse DA-3 cells M2TGWGN6fG:2b4jpZ:Kh[XO|YYm= NFHab5kzOCEQvF2= Mo\IOlAhdWmwcx?= NF;5WnJEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBFSS1|IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDheEAzOCC3TTDh[pRmeiB4MDDtbY5{KGK7IGjUWEBie3OjeR?= NGrE[40zPDlyME[2PC=>
human G361 cells MYXDfZRwfG:6aXRCpIF{e2G7 MVW3NkBp M3PON2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGc{PjFiY3XscJMh[W[2ZYKgO|IhcHK| Ml:4NVc3ODh|OU[=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Chk1 / Chk1 / p-ATR / ATR ; 

PubMed: 26727128     


After Caco-2 cells were treated with or without 1 μ M daunorubicin, whole cell extracts were collected at 1, 3, 6, 12, 24, and 48 h and immunoblotted for Chk1, p-Chk1, ATR, and p-ATR. β-actin served as the loading control.

26727128
Immunofluorescence
p-Chk1; 

PubMed: 26727128     


Caco-2 cells were treated with or without 1 μ M daunorubicin for 48 h, and then fixed and stained with anti-p-Chk1 antibody and DAPI. Images were collected under a fluorescence microscope. Scale bar = 10 μm. 

26727128
Growth inhibition assay
Cell viability; 

PubMed: 30745844     


(A) Viability of AML cell lines under different concentrations of Dnr according to the CCK-8 assay. (B) IC50 of AML cell lines calculated according to the result of the CCK-8 assay.

30745844

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL (177.31 mM)
Water 100 mg/mL (177.31 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 563.98
Formula

C27H29NO10 . HCl

CAS No. 23541-50-6
Storage powder
in solvent
Synonyms Daunomycin HCl
Smiles Cl.COC1=C2C(=O)C3=C(C(=C4CC(O)(CC(OC5CC(N)C(O)C(C)O5)C4=C3O)C(C)=O)O)C(=O)C2=CC=C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03878927 Suspended Drug: CPX-351|Drug: Gemtuzumab Ozogamicin Acute Myeloid Leukemia Weill Medical College of Cornell University|Jazz Pharmaceuticals|Pfizer August 23 2019 Phase 1
NCT03896269 Recruiting Drug: Liposome-encapsulated Daunorubicin-Cytarabine Blasts 10-19 Percent of Bone Marrow Nucleated Cells|Blasts More Than 5 Percent of Bone Marrow Nucleated Cells|High Risk Chronic Myelomonocytic Leukemia|Recurrent Chronic Myelomonocytic Leukemia|Recurrent High Risk Myelodysplastic Syndrome|Refractory Chronic Myelomonocytic Leukemia|Refractory High Risk Myelodysplastic Syndrome M.D. Anderson Cancer Center|National Cancer Institute (NCI) May 14 2019 Phase 1
NCT02914977 Active not recruiting Drug: Daunorubicin Acute Lymphocytic Leukemia|Acute Myeloid Leukemia Tara Lin|University of Kansas Medical Center November 2016 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID