Daunorubicin (RP 13057) HCl

For research use only.

Catalog No.S3035 Synonyms: Daunomycin HCl, Rubidomycin HCl

45 publications

Daunorubicin (RP 13057) HCl Chemical Structure

CAS No. 23541-50-6

Daunorubicin HCl (Daunomycin, RP 13057, Rubidomycin) inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay. Daunorubicin is a topoisomerase II inhibitor that induces apoptosis.

Selleck's Daunorubicin (RP 13057) HCl has been cited by 45 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Daunorubicin HCl (Daunomycin, RP 13057, Rubidomycin) inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay. Daunorubicin is a topoisomerase II inhibitor that induces apoptosis.
Targets
DNA synthesis [1]
(Cell-free assay)
20 nM(Ki)
In vitro

At drug concentrations that reflect the peak plasma concentration after Daunorubicin administration, the primary mechanism is likely to be through interaction with topoisomerase II, which may be a primary triggering event for growth arrest and/or cell killing through a signalling pathway leading to apoptosis, at least in leukemic cells and thymocytes. The quinone structure permits daunorubicin to act as electron acceptors in reactions mediated by oxoreductive enzymes including cytochrome P450 reductase, NADH dehydrogenase, and xanthine oxidase. At Daunorubicin concentrations exceeding approximately 2–4 μM, free radical mediated toxicity and DNA cross-linking may become evident. Daunorubicin inhibits both DNA and RNA syntheses in HeLa cells over a concentration range of 0.2 through 2 μM. Daunorubicin inhibits both DNA syntheses in Ehrlich ascites tumor cells over a concentration range of 4 μM. Daunorubic triggers apoptosis at concentrations of 0.5 and 1 μM in either HL-60 or U-937 human leukemic cells. [1] Daunorubicin stimulates ceramide elevation and apoptosis in P388 and U937 cells through de novo synthesis via activation of the enzyme ceramide synthase. [2] Daunorubicin dose-dependently increases the phosphatidylserine exposure and consequent procoagulant activity of human umbilical vein endothelial cells. Daunorubicin (0.2 mM) significantly enhances the release of endothelial microparticles which are highly procoagulant in human umbilical vein endothelial cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA MB 361(mammary carcinoma cell line) NVvCUWF5S3m2b4TvfIlkyqCjc4PhfS=> M{LufXRp\SClb33wc5Vv\CC5YYOgeIV{fGWmIH\vdkBqdnS{YXPlcIx2dGG{IHP5eI91d3irY3n0fUBi\2GrboP0JG1FSSCPQjCzOlEpdWGvbXHyfUBk[XKlaX7vcYEh[2WubDDsbY5mMSCneIDy[ZN{cW6pIFzhZ3ohMGKndHGt[4Ft[WO2b4Pp[IF{\SluIFnDOVA:OTdibl2= Mn34QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB|OUW0PVAoRjFyM{m1OFkxRC:jPh?=
P388 leukemia cell line NGi1VFhEgXSxdH;4bYPDqGG|c3H5 NIr5fndEd22yb4Xu[EB4[XNiZY\hcJVifGWmIH\vdkBkgXSxdH;4bYNqfHliYXfhbY5{fCCSM{i4JIxmfWunbXnhJINmdGxibHnu[UwhUUN3ME2zJI5O NFi4dIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMES3PVI6Pyd-MUC0O|kzQTd:L3G+
Dx5 w/PSC cell lines NIHNZ29Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mom2WIhmKGOxbYDveY5lKHejczDleoFtfWG2ZXSg[o9zKHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kBFgDVidz;QV2Mh[2WubDDsbY5meyC3c3nu[{BOXFRiYYPzZZktKEmFNUC9NVQhdk1? NH\OUnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zME[5PFQ1QSd-MUC2PVg1PDl:L3G+
MES-SA uterine tumor cell lines NHi2W|hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXjUbIUh[2:vcH;1coQhf2G|IHX2ZYx2[XSnZDDmc5IhfGinIHfyc5d1cCCrbnjpZol1cW:wIH;mJG1GWy2VQTD1eIVzcW6nIIT1cY9zKGOnbHygcIlv\XNidYPpcochVVSWIHHzd4F6NCCLQ{WwQVAvODJzIN88US=> NVrrXHZnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2PVg1PDlpPkGwOlk5PDR7PD;hQi=>
Dx5 cell lines MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NU\ueGE{XGinIHPvcZBwfW6mIIfhd{BmfmGudXH0[YQh\m:{IITo[UBoem:5dHigbY5pcWKrdHnvckBw\iCGeEWgZ4VtdCCuaX7ld{B2e2mwZzDNWHQh[XO|YYmsJIlkPTB;MjFOwG0> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODZ7OES0PUc,OTB4OUi0OFk9N2F-
human epidermoid carcinoma cell line KB-3-1 Mnu5R5l1d3SxeHnjxsBie3OjeR?= NF;jT3hKdiC4aYTyc{BkgXSxdH;4bYMh[WO2aY\peJkhf2G|IHX2ZYx2[XSnZDD1d4lv\yC2aHWgbJVu[W5iZYDp[IVzdW:rZDDjZZJkcW6xbXGgZ4VtdCCuaX7lJGtDNTNvMTygTWM2OD15IH7N NUCzVYxlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK0OFM4PjNpPkGyOFQ{PzZ|PD;hQi=>
human uterine sarcoma cell line MES-SA NVXjZphkS3m2b4TvfIlkyqCjc4PhfS=> Mlf0TY4hfmm2cn:gZ5l1d3SxeHnjJIFkfGm4aYT5JJdieyCndnHseYF1\WRidYPpcochfGinIHj1cYFvKHW2ZYLpcoUhe2G{Y3;tZUBk\WyuIHzpcoUhVUWVLWPBMEBKSzVyPUCuNFch|ryP NWLkOIg4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK0OFM4PjNpPkGyOFQ{PzZ|PD;hQi=>
Jurkat T lymphocytes NF;jRXJEgXSxdH;4bYPDqGG|c3H5 M{nEVGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEq3cnvheEBVKGy7bYDoc4N6fGW|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlA5OjZizszN MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjN4Nk[wNkc,OTZ|Nk[2NFI9N2F-
human HeLa3 cell line M3vRZmN6fG:2b4jpZ:Kh[XO|YYm= MofIR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[TNiY3XscEBtcW6nIHL5JG1VXCCjc4PhfUwhUUN3ME2yMlk3KM7:TR?= M2nKb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUSyNFM4Lz5zNkm0NlA{PzxxYU6=
NCI-H69 cell line Mk\aVJJwdGmoZYLheIlwdiCjc4PhfS=> MlzwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoKHOnboPpeIl3\SCQQ1mtTFY6KGOnbHygcIlv\SCkeTDBcIFu[XJiQnz1[UBie3OjeTygTWM2OD1yLkGg{txO MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzJ6NkO5N{c,OTd{OE[zPVM9N2F-
L1210 MV\GeY5kfGmxbjDhd5NigQ>? NHjvS21KdmirYnn0bY9vKG:oIGLORUB{gW62aHXzbZMhcW5ibX;1d4UhVDF{MUCgZ4VtdHNuIFXEOVAhRSByLkOg{txONg>? MkLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPDJ|MUixK|41OjNzOEG8M4E,
L1210 MmDnSpVv[3Srb36gZZN{[Xl? NFvkU5FKdmirYnn0bY9vKG:oIFTORUB{gW62aHXzbZMhcW5ibX;1d4UhVDF{MUCgZ4VtdHNuIFXEOVAhRSBzIN88UU4> MoP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPDJ|MUixK|41OjNzOEG8M4E,
L1210 NHLDS|JHfW6ldHnvckBie3OjeR?= NHnXepVKdmirYnn0bY9vKG:oIGLORUB{gW62aHXzbZMhcW5ibX;1d4UhVDF{MUCgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCdM1jdeZJq\GmwZTDpcoNwenCxcnH0bY9vNCCHREWwJF0hOC5|IN88UU4> M2HLUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzR7MEWzO{c,PDlyNUO3QE9iRg>?
L1210 MoPrSpVv[3Srb36gZZN{[Xl? M1fadGlvcGmkaYTpc44hd2ZiRF7BJJN6dnSqZYPpd{BqdiCvb4Xz[UBNOTJzMDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFt|SG30bJlucWSrbnWgbY5kd3Kyb4LheIlwdixiRVS1NEA:KDBwNDFOwG0v MnjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPDlyNUO3K|41QTB3M{e8M4E,
L1210 M2jVXWZ2dmO2aX;uJIF{e2G7 MnvrTY5pcWKrdHnvckBw\iCdM1jdeZJq\GmwZTDpcoNwenCxcnH0bY9vKGmwdH:gdJJwdGmoZYLheIlv\yCOMUKxNEBk\WyuczygSWQ2OCB;IECuN|Mh|ryPLh?= NVPMNI46RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxNkexOlQxOSd-NkexOlQxOTxxYU6=
L1210 MnPlSpVv[3Srb36gZZN{[Xl? MoPUTY5pcWKrdHnvckBw\iCdM1jdeIh6dWmmaX7lJIlv[2:{cH;yZZRqd25iaX70c{Bxem:uaX\ldoF1cW6pIFyxNlExKGOnbHzzMEBGTDVyIE2gNE43PiEQvF2u MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek83PzF4NECxK|43PzF4NECxQE9iRg>?
L1210 NYLyfHE5TnWwY4Tpc44h[XO|YYm= NEi3NZpFd3OnIILldZVqemWmIITvJIlvcGmkaYSgeIhmKFKQQTDzfY51cGW|aYOgbY4hdGW3a3XtbYEhVDF{MUCgZ4VtdHNuIFXEOVAhRSByLkOg{txONg>? M1r1WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd{NUK5O|YoRjd{NUK5O|Y9N2F-
L1210 MWHGeY5kfGmxbjDhd5NigQ>? Mk\GSI9{\SC{ZYH1bZJm\CC2bzDpcohq[mm2IITo[UBFVkFic4nueIhme2m|IHnuJIxmfWunbXnhJGwyOjFyIHPlcIx{NCCHREWwJF0hOSEQvF2u M3L6S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd{NUK5O|YoRjd{NUK5O|Y9N2F-
MCF7 NF72WXhEgXSxdH;4bYNqfHliYYPzZZk> MljoOFghcHK| NFjZdVNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjJ{IN88UU4> M{D2NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUmwOFQ4Lz5zN{G5NFQ1PzxxYU6=
MDA-MB-231 MU\DfZRwfG:6aXPpeJkh[XO|YYm= M{XVd|Q5KGi{cx?= MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkK1JO69VS5? NV\kOHRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexPVA1PDdpPkG3NVkxPDR5PD;hQi=>
HeLa NITiXlJEgXSxdH;4bYNqfHliYYPzZZk> NVjUbXZtPDhiaILz M{TtTGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOzJizszNMi=> NG\PPFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G5NFQ1Pyd-MUexPVA1PDd:L3G+
PC-3 NWG0[IJSS3m2b4TvfIlkcXS7IHHzd4F6 MVK0PEBpenN? M2nZWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBENTNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvPDRizszNMi=> NITLXGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G5NFQ1Pyd-MUexPVA1PDd:L3G+
Yoshida sarcoma tumor cells NFToUlBHfW6ldHnvckBie3OjeR?= MV3Dc45k\W62cnH0bY9vKGmwaHnibZRqdmdiW{PIYXRlWiCrbnPvdpBwemG2aX;uJIlvKFmxc3jp[IEhe2G{Y3;tZUB1fW2xcjDj[YxteyCrbjD2bZRzdyCrbjDyZZR{NCCLQ{WwJF0hOC5yMjFOwG0v MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOzJ{OUi2K|4yOzJ{OUi2QE9iRg>?
HL60 MXvBdI9xfG:|aYOgZZN{[Xl? M1rJbGlvKH[rdILvJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5lfWOnIHHwc5B1d3OrczDpckBJVDZyIHPlcIx{NCCDQ{WwJF0hOC5yNDFOwG0v NF3ySW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMki3O|U6Oyd-MUK4O|c2QTN:L3G+
HL60R M2LuRmFxd3C2b4Ppd{Bie3OjeR?= NV:1fGhwUW5idnn0do8h[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmS3Y3WgZZBweHSxc3nzJIlvKEiONkDSJINmdGy|LDDBR|UxKD1iMUCg{txONg>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh5N{W5N{c,OTJ6N{e1PVM9N2F-
HL60 NHrFRZBEgXSxdH;4bYNqfHliYYPzZZk> M{PUUmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JJRzgXCjbjDicJVmKGW6Y3z1d4lwdiCvZYToc4QtKEGFNUCgQUAxNjFizszNMi=> M1z1S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{O5OFU2Lz5zOEOzPVQ2PTxxYU6=
HL60 NF:3dHVCeG:ydH;zbZMh[XO|YYm= MVnJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIH31cJRq\HK3ZzDy[ZNqe3SjboSgbJVu[W5iSFy2NEBk\WyuczDh[pRmeiB2ODDodpMtKEGFNUCgQUA{QCEQvF2u NIOwWVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEOzPVQ2PSd-MUizN|k1PTV:L3G+
HTB-81 NXzVPHlZS3m2b4TvfIlkcXS7IHHzd4F6 MoqzR5l1d3SxeHnjbZR6KHSqYYSgZ4F2e2WmIHGgOVAmKHKnZIXjeIlwdiCrbjDhZpNwemKjbnPlJIF1KDR7MDDucUBqdiCqdX3hckBxem:|dHH0[UBk[W6lZYKgTHRDNThzIHPlcIx{KHW|aX7nJG1VWyCjc4PhfUwhTUN3MDC9JFAvODV6IN88UU4> M1XuZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMEKwNlg2Lz5zMUCyNFI5PTxxYU6=
B16 MWHDfZRwfG:6aXPpeJkh[XO|YYm= M3WyZ2N6fG:2b4jpZ4l1gSC2aHH0JINifXOnZDDhJFUxLSC{ZXT1Z5Rqd25iaX6gZYJ{d3KkYX7j[UBifCB2OUCgco0hcW5iQkG2JI1wfXOnIH3lcIFvd22jIHPlcIx{KHW|aX7nJG1VWyCjc4PhfUwhTUN3MDC9JFAvOTZizszNMi=> Mmn0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTFyMkCyPFUoRjFzMEKwNlg2RC:jPh?=
fibroblast (NHF) cells M2PZUWN6fG:2b4jpZ4l1gSCjc4PhfS=> M3fjemN6fG:2b4jpZ4l1gSC2aHH0JINifXOnZDDhJFUxLSC{ZXT1Z5Rqd25iaX6gZYJ{d3KkYX7j[UBifCB2OUCgco0hcW5ibn;ycYFtKGi3bXHuJIZq[nKxYnzhd5QhME6KRjmgZ4VtdHNidYPpcochVVSVIHHzd4F6NCCHQ{WwJF0hOC5{MjFOwG0v NVTZblNbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUGwNlAzQDVpPkGxNFIxOjh3PD;hQi=>
L1210 MkTjSpVv[3Srb36gZZN{[Xl? NFrYO3A{KGi{cx?= MWfJcohq[mm2aX;uJI9nKESQQTDzfY51cGW|aYOgbY4hdW:3c3WgUFEzOTBiY3XscJMheHKnaX7jeYJifGWmIH\vdkA{KGi{czDmc4xtd3enZDDifUBcO0ifLYTofY1q\GmwZTDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiBzIHjyJIJ6KHOlaX70bYxt[XSrb36gd5Bm[3S{b33leJJq[yCjbnHsfZNqeyxiRVS1NEA:KDBwMzFOwG0v MnTLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4M{W5K|4yPzZ|NUm8M4E,
L1210 Mor1SpVv[3Srb36gZZN{[Xl? NUK0fI1pOyCqcoO= MWDJcohq[mm2aX;uJI9nKFKQQTDzfY51cGW|aYOgbY4hdW:3c3WgUFEzOTBiY3XscJMheHKnaX7jeYJifGWmIH\vdkA{KGi{czDmc4xtd3enZDDifUBcO0ifLYXybYRqdmViYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhOSCqcjDifUB{[2mwdHnscIF1cW:wIIPw[YN1em:vZYTybYMh[W6jbInzbZMtKEWGNUCgQUAxNjNizszNMi=> NF23eHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[zOVkoRjF5NkO1PVww[T5?
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U-2 OS MULxTHRUKGG|c3H5 Mmr6dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFVvMjDPV{Bk\Wyucx?= NGLTNFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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SK-N-SH Moq2dWhVWyCjc4PhfS=> MYrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3TTEBk\Wyucx?= NVnhWYJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 NWjmV2hYeUiWUzDhd5NigQ>? NX3MXHZCeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsdy=> NFGy[489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 M1LDN5FJXFNiYYPzZZk> NVzRenVWeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVEzKGOnbHzz NX[zdJR1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh18 MWfxTHRUKGG|c3H5 MVXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? MlXKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
OHS-50 NG[ybZVyUFSVIHHzd4F6 MU\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| M4nXOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD NGjsVXJyUFSVIHHzd4F6 M2DUcJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>? MlP1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R) MkjPdWhVWyCjc4PhfS=> NVXGO|V[eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF3HJFY{KCh4LWTHJHIqKGOnbHzz M4jy[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
fibroblast cells NVXudJp{eUiWUzDhd5NigQ>? Mlq4dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKGOxboTyc4whUGhid3ns[EB1gXCnIH\pZpJw[myjc4SgZ4VtdHN? NH3UXJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh41 NH3xWFVyUFSVIHHzd4F6 NFvjdoJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUni0NUBk\Wyucx?= MkXRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
P388 NH3ST5BCdnSrdIXtc5Ih[XO|YYm= M3v4WVAvOjVidH:gNVAhdWdxa3e= NUjzO3VUSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhdW:3c3WgVFM5QCClZXzsd{BidGyxZ4Lh[pRm\CCrbjDtc5V{\SCjc4Pld5Nm\CCjczD0eY1weiCpcn;3eIghcW6qaXLpeIlwdiCjdDCwMlI2KHSxIEGwJI1oN2up MlO5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ3NU[2NUc,OTJ3NU[2NVww[T5?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Chk1 / Chk1 / p-ATR / ATR ; 

PubMed: 26727128     


After Caco-2 cells were treated with or without 1 μ M daunorubicin, whole cell extracts were collected at 1, 3, 6, 12, 24, and 48 h and immunoblotted for Chk1, p-Chk1, ATR, and p-ATR. β-actin served as the loading control.

26727128
Immunofluorescence
p-Chk1; 

PubMed: 26727128     


Caco-2 cells were treated with or without 1 μ M daunorubicin for 48 h, and then fixed and stained with anti-p-Chk1 antibody and DAPI. Images were collected under a fluorescence microscope. Scale bar = 10 μm. 

26727128
Growth inhibition assay
Cell viability; 

PubMed: 30745844     


(A) Viability of AML cell lines under different concentrations of Dnr according to the CCK-8 assay. (B) IC50 of AML cell lines calculated according to the result of the CCK-8 assay.

30745844

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL (177.31 mM)
Water 100 mg/mL (177.31 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 563.98
Formula

C27H29NO10 . HCl

CAS No. 23541-50-6
Storage powder
in solvent
Synonyms Daunomycin HCl, Rubidomycin HCl
Smiles CC1C(C(CC(O1)OC2CC(CC3=C2C(=C4C(=C3O)C(=O)C5=C(C4=O)C(=CC=C5)OC)O)(C(=O)C)O)N)O.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04562792 Recruiting Drug: Daunorubicin Relapsed Pediatric ALL|Relapsed Pediatric AML|Refractory Acute Myeloid Leukemia|Refractory Acute Lymphoblastic Leukemia Children''s Mercy Hospital Kansas City May 8 2020 Phase 2
NCT03878927 Recruiting Drug: CPX-351|Drug: Gemtuzumab Ozogamicin Acute Myeloid Leukemia Weill Medical College of Cornell University|Jazz Pharmaceuticals|Pfizer August 23 2019 Phase 1
NCT03896269 Recruiting Drug: Liposome-encapsulated Daunorubicin-Cytarabine Blasts 10-19 Percent of Bone Marrow Nucleated Cells|Blasts More Than 5 Percent of Bone Marrow Nucleated Cells|High Risk Chronic Myelomonocytic Leukemia|Recurrent Chronic Myelomonocytic Leukemia|Recurrent High Risk Myelodysplastic Syndrome|Refractory Chronic Myelomonocytic Leukemia|Refractory High Risk Myelodysplastic Syndrome M.D. Anderson Cancer Center|National Cancer Institute (NCI) May 14 2019 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID