Brefeldin A

For research use only.

Catalog No.S7046

40 publications

Brefeldin A  Chemical Structure

CAS No. 20350-15-6

Brefeldin A is a lactone antibiotic and ATPase inhibitor for protein transport with IC50 of 0.2 μM in HCT 116 cells, induces cancer cell differentiation and apoptosis. It could also improve the HDR(homology-directed repair) efficiency and be an enhancer of CRISPR-mediated HDR. Brefeldin A is also an inhibitor of autophagy and mitophagy.

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Selleck's Brefeldin A has been cited by 40 publications

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Biological Activity

Description Brefeldin A is a lactone antibiotic and ATPase inhibitor for protein transport with IC50 of 0.2 μM in HCT 116 cells, induces cancer cell differentiation and apoptosis. It could also improve the HDR(homology-directed repair) efficiency and be an enhancer of CRISPR-mediated HDR. Brefeldin A is also an inhibitor of autophagy and mitophagy.
Targets
ATPase (HCT 116) [1]
0.2 μM
In vitro

Brefeldin A is a fungal metabolite and blocks the forward transport between the endoplasmic reticulum and Golgi apparatus, Brefeldin A causes an impaired distribution of the membrane proteins. When HCT 116 human colon cancer cell is treated with Brefeldin A, morphological changes indicating cell differentiation are observed. Brefeldin A exerts its cytotoxic effects mainly by inducing differentiation and apoptosis in tumor cells. [1] The treatment of the strips with 20 μg/mL Brefeldin A for 6 hours completely abolishes the relaxation induced by bradykinin in the presence of 10mM indomethacin and 30 μM L-NOARG. The treatment with 20 μg/mL Brefeldin A substantially abolishes the bradykinin-induced decreases in [Ca2+]i and tension in the range of concentrations between 1 nM and 1 mM. Brefeldin A has no effect on the [Ca2+]i elevation in endothelial cells induced by bradykinin or substance P. [2] Addition of the fungal metabolite Brefeldin A does not affect the spontaneous phospholipid-dependent GTPS binding to myr-rARF1 but totally abolishs the retinal isotonic extract (RIE)-catalyzed exchange, with half-maximal inhibition at 2 μM Brefeldin A. Brefeldin A prevents a wide variety of membrane traffic pathways. Brefeldin A inhibits an ADP-ribosylation factor-specific guanine nucleotide exchange activity present in Golgi membranes or in brain cytosol. The complete prevention by Brefeldin A strongly suggests that the retinal extract contains an ARF-specific guanine nucleotide exchange factor. Retinal isotonic extract (RIE)-catalyzed GTPS release from both ADP-ribosylation factors (ARFs) is only partly inhibited by Brefeldin A, even at 300 μM. [3] Brefeldin A induces fusion of the Golgi apparatus with the ER. Brefeldin A abolishes the inhibitory effect of the CERT inhibitor HPA-12. Brefeldin A treatment, which induces fusion of the Golgi apparatus and the ER, rescues the limonoid-induced prevention of sphingomyelin biosynthesis. BFA treatment of CHO cells causes a 2 to 3 fold increase in sphingomyelin synthesis. [4] Apart from B-CLL cells, Brefeldin A reportedly causes apoptosis in multiple myeloma (U266, NCI-H929), Jurkat, HeLa, leukaemia (HL60, K562, BJAB), colon (HT-29) and prostate, as well as adenoid cystic sarcoma cells. The administration of 25 ng/mL of Brefeldin A completely blocks growth of HF4.9 and HF28RA cells, whereas higher Brefeldin A doses (75 ng/mL) are required to achieve the same effect in HF1A3 cells. Cell proliferation is inhibited within 24 hours in a dose-dependent manner and, depending on the cell line, almost complete cessation of 3H-thymdine incorporation is observed at 50-75 ng/mL of Brefeldin A (26%, 76%, 87% inhibition at 50 ng/ml and 75%, 87%, 92% inhibition at 75 ng/mL for HF1A3, HF4.9 and HF28RA cells respectively. Brefeldin A-induced cell killing is in a dose-dependent manner using YO-PRO 1/PI assay. [5] Brefeldin A could improve the HDR(homology-directed repair) efficiency. It is an enhancer of CRISPR-mediated HDR[6].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC12 M{PaWWZ2dmO2aX;uJGF{e2G7 MlnQNkDPxE1? Mnr0NUBp NHTnV5RqdmirYnn0d{B1cGViTD3EU3BCKCh{MNMg{txOMS2rbnT1Z4VlKHS{YX7zbYVvfCCHUluxM|IheGixc4Doc5J6dGG2aX;uxsA> M2fZfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{[zNVkyLz5{NkO2N|E6OTxxYU6=
C2C12 NHnRW|hHfW6ldHnvckBCe3OjeR?= M{TpRlHDqM7:Zz;tcC=> NI\OfoIyyqCq NILhcpZi[m:uaYPo[ZMh[3m2b3vpcoUhemWuZXHz[UBnem:vIFOyR|EzKG27b4T1ZoV{ NVS0OW53RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yPVEzPzlpPkK2NlkyOjd7PD;hQi=>
MEFs WT MkX3SpVv[3Srb36gRZN{[Xl? M2DSTFXDqM7:TR?= MlnJNlAhdWmw MmjyZ4F2e2W|IILld4ll\W62IHXufplu\XNic4XjbEBieyCQQVfUMWdHWCxidH:g[Iln\nW|ZTDiZYNsKHSxIITo[UBGWg>? M{HQWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUm2NFI{Lz5{NkG5OlAzOzxxYU6=
MEFs VAMP7 KO NEXO[nlHfW6ldHnvckBCe3OjeR?= MWW1xsDPxE1? NG\xWm4zOCCvaX6= Mn2wZ4F2e2W|IILld4ll\W62IHXufplu\XNic4XjbEBieyCQQVfUMWdHWCxidH:g[Iln\nW|ZTDiZYNsKHSxIITo[UBGWg>? MnTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzOU[wNlMoRjJ4MUm2NFI{RC:jPh?=
SMCs MlXPSpVv[3Srb36gRZN{[Xl? M1WxTFExyqEEtXevcYw> MnfyNE0yOiCq MV\EUXNQ Mmf4d4hwf3NiYTD0doVv\CC2b4fhdoR{KGFiaHnnbIVzKGOxbnPlcpRz[XSrb36gc4YhfGinIFXSM3NTKG6ndIfvdoshcW5idHjlJJBmemmwdXPs[YFzKGG{ZXJCpC=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5MkC4NEc,OjZzN{KwPFA9N2F-
SMCs MoLrSpVv[3Srb36gRZN{[Xl? M13RVFExyqEEtXevcYw> NFTieZMxNTF{IHi= M2jzN2ROW09? NFL1dldk[XW|ZYOgZUB1emGwc3nlcpQhS2F{K9MgdoVt\WG|ZTDmdo9uKHSqZTDFVk9UWsLi M17hfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUeyNFgxLz5{NkG3NlA5ODxxYU6=
HEMC-1 MUPGeY5kfGmxbjDBd5NigQ>? M3PD[lAvOcLiwsXnM41t MnTHNlTDqGh? NYPUXo1I[2G3c3XzJIEhcGmpaHXyJIlvcGmkaYTvdpkh\W[oZXP0JI9vKGW6b3P5eI9{cXNidHjhckBvd2OxZHH6c4xm MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl5Mke1PUc,OjV7N{K3OVk9N2F-
HUVEC MkmwSpVv[3Srb36gRZN{[Xl? NVuyNlZSOTEEoN88US=> MljFNeKhcA>? MV\EUXNQ NXm3eWRi[WKxbHnzbIV{KGi7cH;4bYEucW6mdXPl[EBz\WynYYPlJI9nKEGWUDDmdo9uKGGyaXPhcEBidmRiYnHzc4xifGW{YXygd5Vz\mGlZYO= Mm\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NU[5PFgoRjJ3OUW2PVg5RC:jPh?=
HUVEC MUfGeY5kfGmxbjDBd5NigQ>? M1vFSlExyqEQvF2= MXSxxsBp NG\PbI5FVVOR NEn3VlNqdmO{ZXHz[ZPDqHSqZTDueY1j\XJiYX7kJIlvfGWwc3n0fUBw\iCobIXvdoV{[2WwdDDhdoVieyCnc4DlZ4lidGy7IHnuJJBmemmwdXPs[YFzKHOyYXPl NHXUbnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm1Olk5QCd-MkW5OVY6QDh:L3G+
Caco-2 NXLzbYxLTnWwY4Tpc44hSXO|YYm= M2SxclIvPSEQvF2= M1TQXlMxKG2rbh?= NVvMdmtb[XS2ZX71ZZRmeyC2aHWgWGdHNc7{MT3t[YRq[XSnZDDpcoNz\WG|ZTDpckBUTVKWIH\1coN1cW:w MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl3NEmzNUc,OjV7NUS5N|E9N2F-
NRK NF3pNllHfW6ldHnvckBCe3OjeR?= NIS2dYYzODEkgJXu[{9udA>? M3XafFTjiIWq NXzvboFnTE2VTx?= M133c5Jme2O3ZYOgcYl1d3SrYzDwdo9oemW|c3nvci=> Mnf0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NEi1PFYoRjJ3OUS4OVg3RC:jPh?=
HeLa M4rZVWZ2dmO2aX;uJGF{e2G7 NFzjZ4EzODEkgJXu[{9udA>? MVmzJIg> MYDEUXNQ MmnlbY5lfWOnczD0bIUh[XK2aX\pZ4lidCCkcnXhb{12eCCxZjD0bIUhT2:uZ3mgZ49ueGyneB?= NU\mblNSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OFg2QDZpPkK1PVQ5PTh4PD;hQi=>
COS NELYTpZHfW6ldHnvckBCe3OjeR?= Mn:wNUDPxGdxbXy= M4fLcVMhcA>? MlrWZ49ueGyndHXsfUBlcXOyZYLz[ZMhfGinIFHQMVEhe2mpbnHsxsA> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTlzNUmwNEc,OjV7MUW5NFA9N2F-
DF1  NYHuWmlyTnWwY4Tpc44hSXO|YYm= NH:yNVEyyqEQvF5CpC=> NHu0c4M1QCCq M{nkdGROW09? MXrkbZNx\XK|ZYRCpJRp\SCneH;n[Y5wfXNiQ2PHZYxPSWOWMjDwdo91\Wmw M13LclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEC3NFU1Lz5{NUiwO|A2PDxxYU6=
nHDFs  NWP1UpR5TnWwY4Tpc44hSXO|YYm= NF:2PVUyyqEQvF5CpC=> NX3oVHRZOsLiaB?= NHfMXVdxemW4ZX70d{B1cGViYYPz[Y1jdHlib3[gZ5l1d3OxbHnjJINw[XRicILveIVqdnNib370c{BId2ypaTDt[Y1jemGwZYO= M4TPe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{eyOlE3Lz5{NUe3NlYyPjxxYU6=
FRT  MnLZSpVv[3Srb36gRZN{[Xl? M2XxVlUh|rypL33s NEfrZlAzyqCq MWLicI9kc3NidILh[oZq[2urbnegeIhzd3WpaDD0bIUhT2:uZ3mgZ49ueGyneDDifUBqdmirYnn0bY5oKEWULYTvMWdwdGerIITyZY5{eG:{dB?= MnLHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NkexNVUoRjJ3N{[3NVE2RC:jPh?=
FRT  NIKydWlHfW6ldHnvckBCe3OjeR?= NH7ESmI2KM7:Zz;tcC=> NHHFT5czyqCq NGn6O2lxemW4ZX70d{B1cGViaX7jdoVie2ViaX6gZ4xm[X[nZDFOtUB{fWK3bnn0d{B4cGWwIGvOZUteccLid3HzJJJm\HWlZXS= Mo\EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NkexNVUoRjJ3N{[3NVE2RC:jPh?=
HepG2  NYDqcZdiTnWwY4Tpc44hSXO|YYm= M{e2clHjiIoEtV5CpC=> MXGyOEBp NFfUV29FVVOR M1OybYRm[3KnYYPld{B1cGVibHX2[Ywhd2ZiUGjSJI1TVkF? M{XDZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkG2OVk4Lz5{NU[xOlU6PzxxYU6=
SMCs NIHreWJHfW6ldHnvckBCe3OjeR?= NILEbXQy|rypL33M NFTWNmc{KGh? M3LGSoFk[3WvdXzheIV{KEOQUGmyJJBzd3SnaX6gbY4hfGinIFXSJINwdXCjcoTt[Y51KGGwZDDuc{Btd26pZYKgZ48udG:lYXzpfoVlKHerdHigeIhmKEexbHfpJI1iemunctMg MnPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OEm0NlUoRjJ3NUi5OFI2RC:jPh?=
OB-6 M{XzZmFxd3C2b4Ppd{BCe3OjeR?= MlTnNk44yqEQvF2= M2jjSFQ5KGh? MnLEbY5lfWOnczDhdI9xfG:|aYO= NHG2WGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWzNlQ5OCd-MkW1N|I1QDB:L3G+
iPSC-CMs  MlHOSpVv[3Srb36gRZN{[Xl? M1zacVUxOCCwZz;tcC=> MlL0OFghcA>? NEPBWHlqdmO{ZXHz[ZMhfGinIHnueIVve2m2eTDv[kB1cGViaHnnbIVzKG2xYnnsbZR6KEyDTWDzJIF1KHSqZTDjc5N1KG:oIITo[UBtd3encjDtc4JqdGm2eTDzdIVkcWW| M4HXSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEi4OlY3Lz5{NUS4PFY3PjxxYU6=
SP-Nluc MlztSpVv[3Srb36gRZN{[Xl? MVy1JI1oN22O MUe2JIg> M2H2WGROW00EoB?= MkXSZ4F2e2W|IHHuJIlv[3KnYYPlJIlvKHKncH;yeIVzKGGldHn2bZR6KGmwIITo[UBx[XKjc3n0[S=> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN7Mkm5PEc,OjV|OUK5PVg9N2F-
PEXEL-Nluc MojpSpVv[3Srb36gRZN{[Xl? MYG1JI1oN22O MmTCOkBp NEnIcmxFVVORwrC= NUf0Opc3[2G3c3XzJIFvKGmwY4LlZZNmKGmwIILldI9zfGW{IHHjeIl3cXS7IHnuJJRp\SCyYYLhd4l1\Q>? M4DvWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{myPVk5Lz5{NUO5Nlk6QDxxYU6=
H1299 M3qwbGZ2dmO2aX;uJGF{e2G7 NFnoTIwyOCEQvHevcYw> NGn5NIIzPCCq Ml3mbY5lfWOnczDheZRweGijZ4pCpC=> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN6OEm3NEc,OjV|OEi5O|A9N2F-
MDA-MB-231 NEDQZolE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MViw5qCUPTBizsznM41N NYC5WnRDPDhiaB?= M{e0eGVEPTEEoE2gNE4xOTZiwsXnM41N NGTWUY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO1OlU3Pyd-MkWzOVY2Pjd:L3G+
MDA-MB-231 NXLjdIx4SXCxcITvd4l{KEG|c3H5 MknZNE4yKM7:Zz;tUC=> M3raPVQhcA>? NF3VWJVqdmS3Y3XzJIFxd3C2b4Ppdy=> NF7lZ2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO1OlU3Pyd-MkWzOVY2Pjd:L3G+
MDA-MB-231 NYiyTnpsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFX0fGcxNjBzL{CuNFUh|rypL33M NGnKenYzPCCq Mn7wbY5kemWjc3XzJJRp\SCocnHjeIlwdiCxZjDzeYIuTzFiY3XscEBl\WK{aYO= M{\leVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{W2OVY4Lz5{NUO1OlU3PzxxYU6=
MDA-MB-231 MUnBdI9xfG:|aYOgRZN{[Xl? M3;vZVAvODYkgKOxJO69\y:vTB?= Moi0NlQhcA>? M{SzeYlv\HWlZYOgVGFTWCBqcH;sfUBCTFBvcnnic5NmKHCxbInt[ZJie2VvMTmgZ4xm[X[jZ3W= NYLnTYVYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzOVY2PjdpPkK1N|U3PTZ5PD;hQi=>
MDA-MB-231 NGrVfoZHfW6ldHnvckBCe3OjeR?= NELZbIEx6oDVNUCg{txoN22O MmTlNlQhcA>? M13uWYlvcGmkaYTzJJRp\SCob4LtZZRqd25ib3[gN2Qh[W6mIELEJINwdG:waXXz NVG4fHpuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzOVY2PjdpPkK1N|U3PTZ5PD;hQi=>
A172 M3;KS2Z2dmO2aX;uJGF{e2G7 NVPxSIdxOTEEoN88[{9udA>? NIPvWXM1yqCq Mn\nSG1UV8Li NVH6e2ZUemW|dXz0d{BqdiC2aHWgdoV1em:pcnHk[UB1emGwc4DvdpQhd2ZiZnz1c5Jme2OnboSg[5JidnWuZYO= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ|OUWwO{c,OjV{M{m1NFc9N2F-
KMS-6 Ml\SSpVv[3Srb36gRZN{[Xl? MYqxxsDPxE4EoB?= Mlm1NlQhcA>? NFTMZnpmgGirYnn0d{Bp[WyoIITo[UB{\WO{ZYTpc44hd2ZiZ3HsZY5qdi2OSTDhd{BlcWRidHjlJINwdnS{b3y= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ{OUGyOkc,OjV{MkmxNlY9N2F-
MEC M3[5dWZ2dmO2aX;uJGF{e2G7 M4PQO|Eh|ryP MYGxMlUhcA>? NHXjNVVk[XW|ZYOgZUBlemGvYYTpZ{Bl\WO{ZXHz[UBqdiC2aHWgd5Vz\mGlZTDWSWdHWjJ? MnzKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{Mki4NVUoRjJ3MkK4PFE2RC:jPh?=
HEK293/hERG M3W3OWZ2dmO2aX;uJGF{e2G7 NWflSHJ4OTEEoN88US=> MXKxxsBp MUPy[ZN2dHS|IHnuJIEhfGmvZT3k[ZBmdmSnboSgdoVlfWO2aX;uJI1ifHW{ZTDoSXJIKHC{b4TlbY7DqA>? M4nlS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkG4OFY6Lz5{NUKxPFQ3QTxxYU6=
RBE4 Mo[wRZBweHSxc3nzJGF{e2G7 M1vyNlLDqM7:TR?= NIjzTlE{6oDVMkVCpIg> M2X4T4lv\HWlZYOgZZBweHSxc3nzJJRqdWViZHXw[Y5l\W62bIm= NF7WcZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyPFAzPSd-MkWxNlgxOjV:L3G+
RBE4 Mk\wSpVv[3Srb36gRZN{[Xl? MoHDNuKh|ryP NVfyNplLO+LCk{K0xsBp NVW2OIp1cW6lcnXhd4V{KHSqZTDYRnAyKHC{b4TlbY4hdGW4ZXzzJIFnfGW{IEOgZY5lKDcEoHigc4YhfHKnYYTt[Y51 MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF{OECyOUc,OjVzMkiwNlU9N2F-
RBE4 Mn[0SpVv[3Srb36gRZN{[Xl? M2P5TVLDqM7:TR?= MVWz5qCUOjUEoHi= MWLpcoNz\WG|ZYOgZYN1cX[nIHPhd5Bie2VvMUKgbY4h[SC2aX3lMYRmeGWwZHXueEBu[W6wZYNCpC=> NFrkcI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyPFAzPSd-MkWxNlgxOjV:L3G+
RBE4 MWDGeY5kfGmxbjDBd5NigQ>? NYS0VpZWOsLizszN MWKz5qCUOjUEoHi= MnvWbY5kemWjc3XzJJRp\SCuZY\lcJMhd2ZiUl;TJJRqdWVvZHXw[Y5l\W62bIm= NX30T4JMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlgxOjVpPkK1NVI5ODJ3PD;hQi=>
RBE4 NWfhTXg2TnWwY4Tpc44hSXO|YYm= NWHZepRDOsLizszN NW\WRVNwO+LCk{K0xsBp MnSwbY5lfWOnczDhJIRmdGG7ZXSg[IVxdGW2aX;uJI9nKHSqZTDFVkBE[TJtwrDjc451\W62IHH0JFbDqGhib3[gbY5kfWKjdHnvckB{cWewaX\pZ4FvfGy7 NVHWXJRCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlgxOjVpPkK1NVI5ODJ3PD;hQi=>
RBE4 NFX4OGpHfW6ldHnvckBCe3OjeR?= NYL0[VVTOsLizszN MkHwN-KBmzJ2wrDo MnGxbY5lfWOnczDhckBwfmW{bH;h[EBw\iCFYUKrxsBqdiC2aHWgcYl1d2Oqb37kdoliKGmwIITo[UBncXK|dDC2xsBpKG:oIHnuZ5Vj[XSrb36gLJDjiIl:4pEJNE4xODFrIHL1eEBE[TJtwrDs[ZZmdHNiaX6geIhqeyCxcnfhcoVtdGViZHXjdoVie2WmIHHmeIVzKDF{wrDoJI9nKGmwY4XiZZRqd25? NF7PWGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyPFAzPSd-MkWxNlgxOjV:L3G+
Huh-7  NEj6VnlHfW6ldHnvckBCe3OjeR?= NG[zU2Qy|rypL33M NHvIelI{6oDVMkVCpIg> M13sSYlv[3KnYYPld{B1cGVibHX2[Ywhd2ZiQWDFNUBqdiCjIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NGPScnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCyOlE4PCd-MkWwNlYyPzR:L3G+
HepG2  NXv0eFdKTnWwY4Tpc44hSXO|YYm= NIr3dVcy|rypL33M MXiz5qCUOjUEoHi= M3y3cYlv[3KnYYPld{B1cGVibHX2[Ywhd2ZiQWDFNUBqdiCjIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> M3HXfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEK2NVc1Lz5{NUCyOlE4PDxxYU6=
H838-LKB1 M1LBR2Z2dmO2aX;uJGF{e2G7 M1q3VlMxyqCwZz;tcC=> MkHiNVIwOThiaB?= MYrpcoNz\WG|ZYOgeIhmKHC{b4TlbY4hdGW4ZXzzJI9nKEKrUNMg MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTBzMUC4Nkc,OjVyMUGwPFI9N2F-
H838-KDLKB1  MmDxSpVv[3Srb36gRZN{[Xl? NFq3U48{OMLibnevcYw> Mk\GNVIwOThiaB?= Ml3QbY5kemWjc3XzJJRp\SCycn;0[YlvKGyndnXsd{Bw\iCEaWFCpC=> NF7NXGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxNVA5Oid-MkWwNVExQDJ:L3G+
H838-KDLKB1  MX;GeY5kfGmxbjDBd5NigQ>? MXezNOKhdmdxbXy= NFnEVpkyOi9zODDo NHW0eWlqdmO{ZXHz[ZMhfGinIHzleoVteyCxZjDwbI9{eGixconsZZRm\CCnSV[y{tEhMHCqb4PwbI8u\UmIMt8xLS=> NFPTbVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxNVA5Oid-MkWwNVExQDJ:L3G+
3T3-L1 M13C[mZ2dmO2aX;uJGF{e2G7 M3fNd|Uh|rypL33s NFryWlc{OCCvaX6= NF7qO2hucW2rY4OgeIhmKGWoZnXjeJMhd2ZiaX7zeYxqdiCjbnSgZ4F2e2W|IILvZpV{fCCyaH;zdIhwenmuYYTpc44hd2ZiQXv0JEhU\XJiNEezLUBidmRicHjvd5Bpd3K7bHH0bY9vKG:oIFHTNVYxKCiWaIKgOlQzKGGwZDDT[ZIhPTh6KR?= NHfSZlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0N|gzPyd-MkS4OFM5Ojd:L3G+
3T3-L1 NIPKeVBHfW6ldHnvckBCe3OjeR?= MV:1JO69\y:vbB?= NXK1XIFUOzBibXnu M3PtZ5Jm[2GyaYT1cIF1\XNiaX7zeYxqdiCjY4Tpc44hf2m2aDDy[ZNx\WO2IITvJJJm\3WuYYTpcochSWu2IHHjeIl3cXS7IHHu[EBCWzF4MDDwbI9{eGixconsZZRqd25? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2M{iyO{c,OjR6NEO4Nlc9N2F-
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3T3-L1 NIDhVIZHfW6ldHnvckBCe3OjeR?= M1HoS|Uh|rypL33s MUGxxsBp NX\2UYJt[2G3c3XzJJJm\Gm|dILpZpV1cW:wIH;mJGdNXVR2IHL1eEBvd3RiaX7jdoVie2ViaX6g[4x2[2:|ZTD1dJRic2V? MkniQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEO4NlcoRjJ2OESzPFI4RC:jPh?=
3T3-L1 NXvzblV[TnWwY4Tpc44hSXO|YYm= M1\2PFUh|rypL33s NHTRUooyyqCq MmT5Z4F2e2W|IIDoc5NxcG:{eXzheIlwdiCxZjD0bIUhTm:6T{GgeJJidnOlcnnweIlwdiCoYXP0c5I> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2M{iyO{c,OjR6NEO4Nlc9N2F-
HeLa  NEL6NpJHfW6ldHnvckBCe3OjeR?= MVi1JO69\y:vbB?= M33r[|MhcA>? NF\mdWxk[XW|ZYOgcpVkdGWjcjDlfINtfXOrb36gc4YhfGinIF\vfG8yKHS{YX7zZ5JqeHSrb36g[oFkfG:{IHHu[EBl\WO{ZXHz[ZMhfHKjboPjdolxfGmxbjDv[kBHd3iRMT3y[Yd2dGG2ZXSg[4Vv\XN? NI\m[Y89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0N|gzPyd-MkS4OFM5Ojd:L3G+
HEK293 NWO5[ndLTnWwY4Tpc44hSXO|YYm= MWe1xsDPxGdxbXy= NHezdIoyOsLiaB?= NVXORnZK[WKxbHnzbIV{KEOPQT3pcoR2[2WmIFPSSWxFOiC|ZXPy[ZRqd25? Mn\xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4OEe0N|EoRjJ2Nki3OFMyRC:jPh?=
COS-1 NYjOSmNRTnWwY4Tpc44hSXO|YYm= M2fmeFUhyrWpL33s NU\uVVAzOjRiaNMg MXXy[ZN1emmldIOgcI9k[WyrenH0bY9vKG:oIF7CJIlvKHSqZTDw[ZJqdnWlbHXhdkBz\Werb39CpC=> M2Poc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkexO|UyLz5{NE[3NVc2OTxxYU6=
PRP NH6ye4VHfW6ldHnvckBCe3OjeR?= NWHveItxOTBizszN NWXhfJlI[WK{b3fheIV{KFOGRj2x{tEudWWmaXH0[YQhS1iFUkeg[Zh1\XKwYXzpfoF1cW:wwrC= NHSyTnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[2PFc2OCd-MkS2Olg4PTB:L3G+
RAW264.7 M3X5b2Fxd3C2b4Ppd{BCe3OjeR?= M3jVb|Qh|ryP NXvyUodGPDhiaB?= Mn3xZZR1\W63YYTld{B1cGViaX7obYJqfGmxbjDv[kBwgC2ORFytbY5lfWOnZDDhdI9xfG:|aYOgZY5lKHSqZTDmZYNqdGm2YYTpc44hd2ZiY3jvcIV{fGW{b3yg[YZndHW6IHL5JGFkNWiHLUG4RU1PUDJ? NHX3b2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zPVA{Oid-MkS2N|kxOzJ:L3G+
MDMs M4\1WmFxd3C2b4Ppd{BCe3OjeR?= MW[xNQKBkc7:Zz;tcC=> NIKxPXYyOi9zNTDo M4T0PYlv\HWlZYOgZZBweHSxc3nz MkDJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3NU[2PVUoRjJ2NUW2Olk2RC:jPh?=
PMHs  NFj3SnJHfW6ldHnvckBCe3OjeR?= NHq2[mwyOOLCk{Kw5qCK|rypL33s MXmyOQKBkWh? MkC1SG1UVw>? NXTQTI95cW6mdXPl[EBGWiC|dILld5M> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDRyN{K0Nkc,OjR2MEeyOFI9N2F-
PMHs  NWe4[GYySXCxcITvd4l{KEG|c3H5 NGfCbXMyOOLCk{Kw5qCK|rypL33s NGHSdWczPOLCiXi= NWLS[XRVTE2VTx?= M3XLSIlv[3KnYYPld{Bk\WyuIHTlZZRp NYPpTo5zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NFczPDJpPkK0OFA4OjR{PD;hQi=>
HEK293/tau M37sWmZ2dmO2aX;uJGF{e2G7 M4nIfFUh|ryP MlSxNU8zNzRiaB?= Ml:2bY5lfWOnczDHc4xocSCocnHncYVvfGG2aX;uxsA> MmD0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NkiwPFkoRjJ2M{[4NFg6RC:jPh?=
HEK293/tau M1P5cmZ2dmO2aX;uJGF{e2G7 NGK0PI02KM7:TR?= MYWzJIg> M{K0Z4lv\HWlZYOgeIF2KGi7cHXydIhwe3Cqb4L5cIF1cW:w MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4OEC4PUc,OjR|NkiwPFk9N2F-
ADF Ml;YSpVv[3Srb36gRZN{[Xl? MmHzNVAh|ryP MlXTNVYhcA>? MUTpcohq[mm2czD0bIUhYm6FbEKtbY5lfWOnZDD0doFve2yxY3H0bY9vKG:oIFPSWC=> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{OEKzNkc,OjR{MkiyN|I9N2F-
U373  MnjsSpVv[3Srb36gRZN{[Xl? NYX6XWR4OTBizszN M{Xrc|E3KGh? MV3pcohq[mm2czD0bIUhYm6FbEKtbY5lfWOnZDD0doFve2yxY3H0bY9vKG:oIFPSWC=> NYnhN2V[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlgzOzJpPkK0NlI5OjN{PD;hQi=>
RKO-HIPK2i NW[1R|l[TnWwY4Tpc44hSXO|YYm= NF[zN5UyOCEQvF2= MojYNVYhcA>? NUHjWWJVcW6qaXLpeJMhfGinIGruR4wzNWmwZIXj[YQhfHKjboPsc4NifGmxbjDv[kBEWlR? NWjUPYQzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlgzOzJpPkK0NlI5OjN{PD;hQi=>
ADF  MoX3SpVv[3Srb36gRZN{[Xl? MnfJNVAh|ryPwrC= MUK2JIg> NGW1c4pqdXCjaYLzJJRp\SCGQzDhZ5RqfmG2aX;u M1rBVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkK4NlMzLz5{NEKyPFI{OjxxYU6=
Huh7 NIjp[W9HfW6ldHnvckBCe3OjeR?= NFq3N2Y2KM7:Zz;tcC=> M36wTVQhcA>? NUDYVoRp[WKxbHnzbIV{KHSqZTDz[YNz\XSrb36gc4YhKGmwdILhZ4VtdHWuYYKgRZBwSg>? M3r2OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUCwNVQxLz5{NEGwNFE1ODxxYU6=
Huh7 NF\3ZXRHfW6ldHnvckBCe3OjeR?= MXO1JO69\y:vbB?= NHGwO4EyKGh? MULjZZV{\XNiYTDzbYdvcW[rY3HueEBqdmO{ZXHz[UBqdiCDcH;CMYNz\XOlZX70dy=> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyMEG0NEc,OjRzMECxOFA9N2F-
Huh7 M1Oy[mZ2dmO2aX;uJGF{e2G7 M1v0cFXjiJNzMNMgcocwdWx? MnO3NVIhcA>? NWLTb2NocW6lcnXhd4V{KEGyb1KtZ5Jme2OnboTzJJdqfGixdYSgbY5pcWKrdHnu[{B{\WO{ZYTpc44> MmDRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMECxOFAoRjJ2MUCwNVQxRC:jPh?=
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Macrophages NXPhSXZXTnWwY4Tpc44hSXO|YYm= MVO3NUDDvU1? MUe2JIg> MkXYbY5pcWKrdIOgcJVv[XOrbjDpcpRmem6jbHn6ZZRqd28EoB?= MkHoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyM{m3OFAoRjJ2MEO5O|QxRC:jPh?=
Colo 205 NU\KfGZ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVewMVUh|rypL33M NVfGTldJPDhiaB?= M2\MWYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIIP1d5BmdnOrb36gZ5VtfHW{ZYOge4l1cCCjbjDld5RqdWG2ZXSgTWM2OCCxZjD+NVUhdmdxbVy= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl5M{m5Okc,OjN7N{O5PVY9N2F-
Colo 205 MXjGeY5kfGmxbjDBd5NigQ>? Mlr2NE4xOTJvMD6wNlUh|rypL33M MofrNVQh\A>? NXuzU4g3emWmdXPld{B1cGViY3zvco9o\W6rY3n0fUBw\iCFb3zvJFIxPSCFU1Pz MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl5M{m5Okc,OjN7N{O5PVY9N2F-
Colo 205 MU\BdI9xfG:|aYOgRZN{[Xl? MmjRNE4yKM7:Zz;tUC=> Mn\mNE0zPCCq NWXIdJdFcW6mdXPld{BieG:ydH;zbZMhd2ZiQ3;sc{AzODViY3XscJMhcW5ic4XzdIVve2mxbjDjeYx1fXKncx?= NYn3T2Q2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5O|M6QTZpPkKzPVc{QTl4PD;hQi=>
Colo 205 NHiycmFHfW6ldHnvckBCe3OjeR?= NF\w[VUxNjBzNTFOwIcwdUx? MnjhNlQhcA>? M1jGZYlv\HWlZYOgeIhmKGW6cILld5Nqd25ib3[gSXIhe3S{ZYPzMZJmdGG2ZXSg[4Vv\XN? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl5M{m5Okc,OjN7N{O5PVY9N2F-
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p53 / GRP78 ; 

PubMed: 22859938     


(B) present the dose- and time- dependent effect of ER stress inducer brefeldin A on p53 expression. MCF-7 Cells were exposed to brefeldin A in 10% FBS–supplemented DMEM as time and dose indicated. The cell lysates were analyzed by western blotting with antibodies for p53, GRP78, and α-tubulin. The p53 protein expression level was quantified densitometrically. GRP78 served as an ER stress marker. 

22859938
Immunofluorescence
FMNL1 / GM130 ; 

PubMed: 21868368     


FMNL1 colocalizes with the cis-Golgi marker GM130 and is dispersed by treatment with brefeldin A (+BFA).

ErbB3 / Calnexin ; 

PubMed: 21576364     


293T cells transfected with ErbB3 were treated without (top) or with (bottom) BfA, and confocal immunofluorescence microscopy was used to examine the colocalization of ErbB3 with endogenous calnexin.

MTP / GBF1 ; 

PubMed: 26267806     


3T3-L1 cells, plated as above, were treated with brefeldin A (5 μg/ml, 3 hr), fixed and stained for MTP and GBF1. Brefeldin A treatment leads to redistribution of MTP from the juxtanuclear region, similar to what occurs with GBF1. Bars = 10 μm.

21868368 21576364 26267806
Growth inhibition assay
Cell viability; 

PubMed: 28462831     


Effects of brefeldin A on human prostate cancer cells. LNCaP and PC-3 cells were seeded in cell culture dishes and incubated for 24 h. The cells were then treated with different concentrations of brefeldin A for 72 h. The number of viable cells was determined by the trypan blue exclusion assay. Apoptosis was determined by propidium iodide staining and morphological assessment.

28462831

Protocol

Cell Research:

[5]

- Collapse
  • Cell lines: Human follicular lymphoma cell lines HF1A3, HF4.9 and HF28RA
  • Concentrations: 0 ng/mL -75 ng/mL
  • Incubation Time: 5 days
  • Method:

    HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 4 mg/mL (14.26 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 280.36
Formula

C16H24O4

CAS No. 20350-15-6
Storage powder
in solvent
Synonyms N/A
Smiles CC1CCCC=CC2CC(CC2C(C=CC(=O)O1)O)O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID