Brefeldin A

For research use only.

Catalog No.S7046

44 publications

Brefeldin A  Chemical Structure

CAS No. 20350-15-6

Brefeldin A is a lactone antibiotic and ATPase inhibitor for protein transport with IC50 of 0.2 μM in HCT 116 cells, induces cancer cell differentiation and apoptosis. It could also improve the HDR(homology-directed repair) efficiency and be an enhancer of CRISPR-mediated HDR. Brefeldin A is also an inhibitor of autophagy and mitophagy.

Selleck's Brefeldin A has been cited by 44 publications

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Choose Selective ATPase Inhibitors

Biological Activity

Description Brefeldin A is a lactone antibiotic and ATPase inhibitor for protein transport with IC50 of 0.2 μM in HCT 116 cells, induces cancer cell differentiation and apoptosis. It could also improve the HDR(homology-directed repair) efficiency and be an enhancer of CRISPR-mediated HDR. Brefeldin A is also an inhibitor of autophagy and mitophagy.
Targets
ATPase (HCT 116) [1]
0.2 μM
In vitro

Brefeldin A is a fungal metabolite and blocks the forward transport between the endoplasmic reticulum and Golgi apparatus, Brefeldin A causes an impaired distribution of the membrane proteins. When HCT 116 human colon cancer cell is treated with Brefeldin A, morphological changes indicating cell differentiation are observed. Brefeldin A exerts its cytotoxic effects mainly by inducing differentiation and apoptosis in tumor cells. [1] The treatment of the strips with 20 μg/mL Brefeldin A for 6 hours completely abolishes the relaxation induced by bradykinin in the presence of 10mM indomethacin and 30 μM L-NOARG. The treatment with 20 μg/mL Brefeldin A substantially abolishes the bradykinin-induced decreases in [Ca2+]i and tension in the range of concentrations between 1 nM and 1 mM. Brefeldin A has no effect on the [Ca2+]i elevation in endothelial cells induced by bradykinin or substance P. [2] Addition of the fungal metabolite Brefeldin A does not affect the spontaneous phospholipid-dependent GTPS binding to myr-rARF1 but totally abolishs the retinal isotonic extract (RIE)-catalyzed exchange, with half-maximal inhibition at 2 μM Brefeldin A. Brefeldin A prevents a wide variety of membrane traffic pathways. Brefeldin A inhibits an ADP-ribosylation factor-specific guanine nucleotide exchange activity present in Golgi membranes or in brain cytosol. The complete prevention by Brefeldin A strongly suggests that the retinal extract contains an ARF-specific guanine nucleotide exchange factor. Retinal isotonic extract (RIE)-catalyzed GTPS release from both ADP-ribosylation factors (ARFs) is only partly inhibited by Brefeldin A, even at 300 μM. [3] Brefeldin A induces fusion of the Golgi apparatus with the ER. Brefeldin A abolishes the inhibitory effect of the CERT inhibitor HPA-12. Brefeldin A treatment, which induces fusion of the Golgi apparatus and the ER, rescues the limonoid-induced prevention of sphingomyelin biosynthesis. BFA treatment of CHO cells causes a 2 to 3 fold increase in sphingomyelin synthesis. [4] Apart from B-CLL cells, Brefeldin A reportedly causes apoptosis in multiple myeloma (U266, NCI-H929), Jurkat, HeLa, leukaemia (HL60, K562, BJAB), colon (HT-29) and prostate, as well as adenoid cystic sarcoma cells. The administration of 25 ng/mL of Brefeldin A completely blocks growth of HF4.9 and HF28RA cells, whereas higher Brefeldin A doses (75 ng/mL) are required to achieve the same effect in HF1A3 cells. Cell proliferation is inhibited within 24 hours in a dose-dependent manner and, depending on the cell line, almost complete cessation of 3H-thymdine incorporation is observed at 50-75 ng/mL of Brefeldin A (26%, 76%, 87% inhibition at 50 ng/ml and 75%, 87%, 92% inhibition at 75 ng/mL for HF1A3, HF4.9 and HF28RA cells respectively. Brefeldin A-induced cell killing is in a dose-dependent manner using YO-PRO 1/PI assay. [5] Brefeldin A could improve the HDR(homology-directed repair) efficiency. It is an enhancer of CRISPR-mediated HDR[6].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC12 MXnGeY5kfGmxbjDBd5NigQ>? NYW5XXZQOiEQvF2= NFPWTGYyKGh? NFPOO2FqdmirYnn0d{B1cGViTD3EU3BCKCh{MNMg{txOMS2rbnT1Z4VlKHS{YX7zbYVvfCCHUluxM|IheGixc4Doc5J6dGG2aX;uxsA> MorNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|NkOxPVEoRjJ4M{[zNVkyRC:jPh?=
C2C12 NF\KT2ZHfW6ldHnvckBCe3OjeR?= MoHSNeKh|rypL33s MX2xxsBp Mmi2ZYJwdGm|aHXzJIN6fG:taX7lJJJmdGWjc3Wg[pJwdSCFMlOxNkBugW:2dXLldy=> Mnz5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{OUGyO|koRjJ4MkmxNlc6RC:jPh?=
MEFs WT NYHPR4hXTnWwY4Tpc44hSXO|YYm= M{[2O|XDqM7:TR?= MViyNEBucW5? NYXWW3h1[2G3c3XzJJJme2mmZX70JIVvgnmvZYOgd5VkcCCjczDORWdVNUeIUDygeI8h\GmoZoXz[UBj[WOtIITvJJRp\SCHUh?= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF7NkCyN{c,OjZzOU[wNlM9N2F-
MEFs VAMP7 KO NYHXPWJjTnWwY4Tpc44hSXO|YYm= MXi1xsDPxE1? MUCyNEBucW5? MYLjZZV{\XNicnXzbYRmdnRiZX76fY1meyC|dXPoJIF{KE6DR2StS2ZRNCC2bzDkbYZnfXOnIHLhZ4shfG9idHjlJGVT M4P2U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUm2NFI{Lz5{NkG5OlAzOzxxYU6=
SMCs MXnGeY5kfGmxbjDBd5NigQ>? MlzXNVDDqML3Zz;tcC=> MmHKNE0yOiCq MnXTSG1UVw>? NXHaSGRme2ixd4OgZUB1emWwZDD0c5diemS|IHGgbIlocGW{IHPvcoNmdnS{YYTpc44hd2ZidHjlJGVTN1OUIH7leJdwemtiaX6geIhmKHCncnnueYNt\WG{IHHy[YHDqA>? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5MkC4NEc,OjZzN{KwPFA9N2F-
SMCs NGjSVolHfW6ldHnvckBCe3OjeR?= MX6xNOKhyrWpL33s NFfKN4oxNTF{IHi= NWnrclJSTE2VTx?= MmHQZ4F2e2W|IHGgeJJidnOrZX70JGNiOiwEoILlcIVie2ViZoLvcUB1cGViRWKvV3LDqA>? M4K4TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUeyNFgxLz5{NkG3NlA5ODxxYU6=
HEMC-1 M2\EVGZ2dmO2aX;uJGF{e2G7 MWmwMlHDqML3Zz;tcC=> M3joeVI1yqCq MYLjZZV{\XNiYTDobYdp\XJiaX7obYJqfG:{eTDl[oZm[3Rib36g[Zhw[3m2b4Ppd{B1cGGwIH7vZ49l[XqxbHW= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl5Mke1PUc,OjV7N{K3OVk9N2F-
HUVEC NEfIU2FHfW6ldHnvckBCe3OjeR?= MXGxNOKh|ryP Mn;vNeKhcA>? NYHFOnNsTE2VTx?= MmPTZYJwdGm|aHXzJIh6eG:6aXGtbY5lfWOnZDDy[Yxm[XOnIH;mJGFVWCCocn;tJIFxcWOjbDDhcoQh[mG|b3zheIVz[Wxic4Xy[oFk\XN? NV\jT5d4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OVY6QDhpPkK1PVU3QTh6PD;hQi=>
HUVEC NYjDV4IxTnWwY4Tpc44hSXO|YYm= M1rFbFExyqEQvF2= MU[xxsBp MXTEUXNQ M13xUYlv[3KnYYPld:KhfGinIH71cYJmeiCjbnSgbY51\W6|aYT5JI9nKG[udX;y[ZNk\W62IHHy[YF{KGW|cHXjbYFtdHliaX6gdIVzcW63Y3zlZZIhe3CjY3W= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl3Nkm4PEc,OjV7NU[5PFg9N2F-
Caco-2 NGLuVFFHfW6ldHnvckBCe3OjeR?= NH6z[FUzNjVizszN NEL3clM{OCCvaX6= NHq3dItifHSnboXheIV{KHSqZTDUS2Yu|rJzLX3l[IlifGWmIHnuZ5Jm[XOnIHnuJHNGWlRiZoXuZ5Rqd25? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl3NEmzNUc,OjV7NUS5N|E9N2F-
NRK NVu5NJZRTnWwY4Tpc44hSXO|YYm= MWOyNFDjiIWwZz;tcC=> MkjYOQKBjWh? M{nDcmROW09? MonvdoV{[3WnczDtbZRwfGmlIIDyc4dz\XO|aX;u M2HC[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUS4OVg3Lz5{NUm0PFU5PjxxYU6=
HeLa NES3ZoNHfW6ldHnvckBCe3OjeR?= MlXlNlAx6oDHbnevcYw> M2fmcFMhcA>? MU\EUXNQ NUXOO4hmcW6mdXPld{B1cGViYYL0bYZq[2mjbDDidoVicy23cDDv[kB1cGViR3;s[4kh[2:vcHzlfC=> M4PuPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUS4OVg3Lz5{NUm0PFU5PjxxYU6=
COS Mn7wSpVv[3Srb36gRZN{[Xl? Mn;MNUDPxGdxbXy= MoHPN{Bp NGHYboJkd22ybHX0[Yx6KGSrc4DldpNmeyC2aHWgRXAuOSC|aXfuZYzDqA>? MljlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7MUW5NFAoRjJ3OUG1PVAxRC:jPh?=
DF1  NXnrem5uTnWwY4Tpc44hSXO|YYm= M4X0TFHDqM7:TdMg NYHZSI4{PDhiaB?= M3vQRmROW09? M{\YTIRqe3CncoPld:KhfGinIHX4c4dmdm:3czDDV2didE6DY2SyJJBzd3SnaX6= NEPCXZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiwO|A2PCd-MkW4NFcxPTR:L3G+
nHDFs  NEKwRVVHfW6ldHnvckBCe3OjeR?= MWGxxsDPxE4EoB?= NXvSXY5uOsLiaB?= MoTmdJJmfmWwdIOgeIhmKGG|c3XtZox6KG:oIHP5eI9{d2yrYzDjc4F1KHC{b4TlbY5{KG:wdH:gS49t\2libXXtZpJidmW| MlL4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5N{K2NVYoRjJ3N{eyOlE3RC:jPh?=
FRT  MVzGeY5kfGmxbjDBd5NigQ>? MW[1JO69\y:vbB?= MnfGNuKhcA>? MUTicI9kc3NidILh[oZq[2urbnegeIhzd3WpaDD0bIUhT2:uZ3mgZ49ueGyneDDifUBqdmirYnn0bY5oKEWULYTvMWdwdGerIITyZY5{eG:{dB?= M{i1WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{[3NVE2Lz5{NUe2O|EyPTxxYU6=
FRT  MkfCSpVv[3Srb36gRZN{[Xl? M{T3PVUh|rypL33s NHrjZ4ozyqCq NFnyTFlxemW4ZX70d{B1cGViaX7jdoVie2ViaX6gZ4xm[X[nZDFOtUB{fWK3bnn0d{B4cGWwIGvOZUteccLid3HzJJJm\HWlZXS= Ml6wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NkexNVUoRjJ3N{[3NVE2RC:jPh?=
HepG2  M4nINWZ2dmO2aX;uJGF{e2G7 NF\p[oEy6oDLwsXNxsA> MmDwNlQhcA>? MYDEUXNQ M{PwVIRm[3KnYYPld{B1cGVibHX2[Ywhd2ZiUGjSJI1TVkF? Mn3jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4MU[1PVcoRjJ3NkG2OVk4RC:jPh?=
SMCs M4DGS2Z2dmO2aX;uJGF{e2G7 NV63OJI{Oc7:Zz;tUC=> MoD4N{Bp M2T5WIFk[3WvdXzheIV{KEOQUGmyJJBzd3SnaX6gbY4hfGinIFXSJINwdXCjcoTt[Y51KGGwZDDuc{Btd26pZYKgZ48udG:lYXzpfoVlKHerdHigeIhmKEexbHfpJI1iemunctMg M2T6RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUi5OFI2Lz5{NUW4PVQzPTxxYU6=
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iPSC-CMs  MmDYSpVv[3Srb36gRZN{[Xl? NULZV|BqPTByIH7nM41t NWflUmp[PDhiaB?= NIfBTWpqdmO{ZXHz[ZMhfGinIHnueIVve2m2eTDv[kB1cGViaHnnbIVzKG2xYnnsbZR6KEyDTWDzJIF1KHSqZTDjc5N1KG:oIITo[UBtd3encjDtc4JqdGm2eTDzdIVkcWW| NEfGNoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS4PFY3Pid-MkW0PFg3PjZ:L3G+
SP-Nluc M4jJR2Z2dmO2aX;uJGF{e2G7 NVjOZVJNPSCvZz;tUC=> M4robVYhcA>? MVfEUXNQyqB? M1jaOoNifXOnczDhckBqdmO{ZXHz[UBqdiC{ZYDvdpRmeiCjY4Tpeol1gSCrbjD0bIUheGG{YYPpeIU> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN7Mkm5PEc,OjV|OUK5PVg9N2F-
PEXEL-Nluc NIfVfJBHfW6ldHnvckBCe3OjeR?= MYS1JI1oN22O NFW1T5A3KGh? NVHPUFU{TE2VT9Mg MX3jZZV{\XNiYX6gbY5kemWjc3WgbY4hemWyb4L0[ZIh[WO2aY\peJkhcW5idHjlJJBiemG|aYTl MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN7Mkm5PEc,OjV|OUK5PVg9N2F-
H1299 MVnGeY5kfGmxbjDBd5NigQ>? MmLWNVAh|rypL33s M1fuUVI1KGh? MXzpcoR2[2W|IHH1eI9xcGGpedMg MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN6OEm3NEc,OjV|OEi5O|A9N2F-
MDA-MB-231 MlXRR4VtdCCYaXHibYxqfHliQYPzZZk> M1[yWFDjiJN3MDFOwIcwdUx? MXu0PEBp MXPFR|UxyqB;IECuNFE3KML3Zz;tUC=> MmHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|NU[1OlcoRjJ3M{W2OVY4RC:jPh?=
MDA-MB-231 MnmyRZBweHSxc3nzJGF{e2G7 Mmn0NE4yKM7:Zz;tUC=> MXG0JIg> MWnpcoR2[2W|IHHwc5B1d3Orcx?= MmHmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|NU[1OlcoRjJ3M{W2OVY4RC:jPh?=
MDA-MB-231 NELpRWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;DNE4xOS9yLkC1JO69\y:vTB?= MmnvNlQhcA>? MoS2bY5kemWjc3XzJJRp\SCocnHjeIlwdiCxZjDzeYIuTzFiY3XscEBl\WK{aYO= NXjk[VdoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzOVY2PjdpPkK1N|U3PTZ5PD;hQi=>
MDA-MB-231 MWnBdI9xfG:|aYOgRZN{[Xl? NYnocJI1OC5yNfMAl|Eh|rypL33M NHnFbVMzPCCq NU\3R5ZzcW6mdXPld{BRSVKSIDjwc4x6KEGGUD3ybYJwe2VicH;sfY1memG|ZT2xLUBkdGWjdnHn[S=> M2ixNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{W2OVY4Lz5{NUO1OlU3PzxxYU6=
MDA-MB-231 M1zCdWZ2dmO2aX;uJGF{e2G7 M3zZe|DjiJN3MDFOwIcwdUx? MWGyOEBp MYrpcohq[mm2czD0bIUh\m:{bXH0bY9vKG:oIEPEJIFv\CB{RDDjc4xwdmmncx?= NVjIO|lQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzOVY2PjdpPkK1N|U3PTZ5PD;hQi=>
A172 MX\GeY5kfGmxbjDBd5NigQ>? NIL5OXoyOMLizsznM41t MmPFOOKhcA>? M4LEU2ROW00EoB?= M1LoSpJme3WudIOgbY4hfGinIILleJJw\3KjZHWgeJJidnOyb4L0JI9nKG[udX;y[ZNk\W62IHfyZY52dGW| M{\iWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkO5OVA4Lz5{NUKzPVUxPzxxYU6=
KMS-6 MkjSSpVv[3Srb36gRZN{[Xl? NH3N[XMyyqEQvF5CpC=> Ml:4NlQhcA>? NX;6R4tI\XiqaXLpeJMhcGGuZjD0bIUhe2WlcnX0bY9vKG:oIHfhcIFvcW5vTFmgZZMh\GmmIITo[UBkd262cn;s MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ{OUGyOkc,OjV{MkmxNlY9N2F-
MEC MVzGeY5kfGmxbjDBd5NigQ>? NVrwNZJGOSEQvF2= M2nnPVEvPSCq M1LuVYNifXOnczDhJIRz[W2jdHnjJIRm[3KnYYPlJIlvKHSqZTDzeZJn[WOnIG\FS2ZTOg>? M{X2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkK4PFE2Lz5{NUKyPFgyPTxxYU6=
HEK293/hERG MkHwSpVv[3Srb36gRZN{[Xl? M4jWZ|ExyqEQvF2= NVniVWRUOcLiaB?= NE[1NZpz\XO3bITzJIlvKGFidHnt[U1l\XCnbnTlcpQhemWmdXP0bY9vKG2jdIXy[UBpTVKJIIDyc5RmcW8EoB?= MkHoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{MUi0OlkoRjJ3MkG4OFY6RC:jPh?=
RBE4 MXPBdI9xfG:|aYOgRZN{[Xl? NUDlRm1TOsLizszN MmnmN-KBmzJ2wrDo M4exbolv\HWlZYOgZZBweHSxc3nzJJRqdWViZHXw[Y5l\W62bIm= MnLlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkiwNlUoRjJ3MUK4NFI2RC:jPh?=
RBE4 Mn7qSpVv[3Srb36gRZN{[Xl? NYrqSoN3OsLizszN MWiz5qCUOjUEoHi= Mn[xbY5kemWjc3XzJJRp\SC[QmCxJJBzd3SnaX6gcIV3\Wy|IHHmeIVzKDNiYX7kJFbDqGhib3[geJJm[XSvZX70 NHriN5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyPFAzPSd-MkWxNlgxOjV:L3G+
RBE4 NHjrbY1HfW6ldHnvckBCe3OjeR?= MmfENuKh|ryP NVLxWlQ4O+LCk{K0xsBp NFi5UVJqdmO{ZXHz[ZMh[WO2aY\lJINie3Cjc3WtNVIhcW5iYTD0bY1mNWSncHXu[IVvfCCvYX7u[ZLDqA>? NX3aVnQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlgxOjVpPkK1NVI5ODJ3PD;hQi=>
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HepG2  NYLLOFBnTnWwY4Tpc44hSXO|YYm= M3PyTFHPxGdxbVy= NULYNJpDO+LCk{K0xsBp NWizVGZ4cW6lcnXhd4V{KHSqZTDs[ZZmdCCxZjDBVGUyKGmwIHGgeIlu\S2mZYDlcoRmdnRibXHucoVz M4XmPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEK2NVc1Lz5{NUCyOlE4PDxxYU6=
H838-LKB1 NHi4cVFHfW6ldHnvckBCe3OjeR?= M1HE[lMxyqCwZz;tcC=> NXOxdI1MOTJxMUigbC=> MXnpcoNz\WG|ZYOgeIhmKHC{b4TlbY4hdGW4ZXzzJI9nKEKrUNMg NHzVSG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxNVA5Oid-MkWwNVExQDJ:L3G+
H838-KDLKB1  NVnJeFVoTnWwY4Tpc44hSXO|YYm= MnXVN|DDqG6pL33s NFrzNGkyOi9zODDo MmnPbY5kemWjc3XzJJRp\SCycn;0[YlvKGyndnXsd{Bw\iCEaWFCpC=> NGTXd4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxNVA5Oid-MkWwNVExQDJ:L3G+
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HEK293 MYPGeY5kfGmxbjDBd5NigQ>? MoGxOeKh|rypL33s NFPR[WwyOsLiaB?= MX\hZo9tcXOqZYOgR21CNWmwZIXj[YQhS1KHTFSyJJNm[3KndHnvci=> NHftN|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[4O|Q{OSd-MkS2PFc1OzF:L3G+
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RAW264.7 NFTnS5dCeG:ydH;zbZMhSXO|YYm= MXG0JO69VQ>? MkDDOFghcA>? MUjheJRmdnWjdHXzJJRp\SCrbnjpZol1cW:wIH;mJI95NUyGTD3pcoR2[2WmIHHwc5B1d3OrczDhcoQhfGinIH\hZ4ltcXSjdHnvckBw\iClaH;s[ZN1\XKxbDDl[oZtfXhiYomgRYMucEVvMUjBMW5JOg>? MlvzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{mwN|IoRjJ2NkO5NFMzRC:jPh?=
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HEK293/tau M3LIWGZ2dmO2aX;uJGF{e2G7 M3;6dFUh|ryP M2joOFEwOi92IHi= Mn\KbY5lfWOnczDHc4xocSCocnHncYVvfGG2aX;uxsA> MkC1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NkiwPFkoRjJ2M{[4NFg6RC:jPh?=
HEK293/tau NEDR[2ZHfW6ldHnvckBCe3OjeR?= NIKw[ZM2KM7:TR?= NEn0TlI{KGh? NHrWbmRqdmS3Y3XzJJRifSCqeYDldpBpd3OyaH;yfYxifGmxbh?= MnjBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NkiwPFkoRjJ2M{[4NFg6RC:jPh?=
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Bel7402 M{TEbGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGrxfVY4OiCqcoO= MlziRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEZXy3OFAzKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6wNlTPxE1w NHLEc4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWyOFczQCd-Mkm1NlQ4Ojh:L3G+
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Bel7402/5-FU NEDnSXRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4n1UlczKGi{cx?= MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKnbEe0NFIwPS2IVTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvQDMQvF2u M{XnU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUK0O|I5Lz5{OUWyOFczQDxxYU6=
HeLa MlnuSpVv[3Srb36gZZN{[Xl? MoLSNVghfU1? NIXNO3c{KGi{cx?= NEL4c2dKdmirYnn0bY9vKG:oIHHsb4FtcW6nIIDoc5NxcGG2YYPlJJNm[3KndHnvckBqdiCqdX3hckBJ\UyjIHPlcIx{KGG2IEG4JJVOKGmwY4XiZZRm\CCob4KgN{BpenN? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTR{MUm2OUc,OzF2MkG5OlU9N2F-
VERO-E6 M{[zXWZ2dmO2aX;uJIF{e2G7 MoP5OFghcHK| MXnE[ZRmem2rbnH0bY9vKG:oIFnDOVAhfmGudXXzJIZweiCrbnjpZol1cW:wIH;mJHNCWlNvQ3;WMVIhcW6mdXPl[EBkgXSxdH;4bYNqfHlib3[gWmVTVy2HNjDj[YxteyCjZoTldkA1QCCqb4Xyd{Ah\Xiyb4P1doUhfG9iMD6wNUBOV0liU1HSV{BEd1ZvMjD2bZJ2eyCkeTDobYdpKGOxboTlcpQhcW2jZ3nu[{whUUN3ME2wMlAz|ryPLh?= NIjxVXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQTl6MD:nQmNpTU2ETEyvZV4>
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p53 / GRP78 ; 

PubMed: 22859938     


(B) present the dose- and time- dependent effect of ER stress inducer brefeldin A on p53 expression. MCF-7 Cells were exposed to brefeldin A in 10% FBS–supplemented DMEM as time and dose indicated. The cell lysates were analyzed by western blotting with antibodies for p53, GRP78, and α-tubulin. The p53 protein expression level was quantified densitometrically. GRP78 served as an ER stress marker. 

22859938
Immunofluorescence
FMNL1 / GM130 ; 

PubMed: 21868368     


FMNL1 colocalizes with the cis-Golgi marker GM130 and is dispersed by treatment with brefeldin A (+BFA).

ErbB3 / Calnexin ; 

PubMed: 21576364     


293T cells transfected with ErbB3 were treated without (top) or with (bottom) BfA, and confocal immunofluorescence microscopy was used to examine the colocalization of ErbB3 with endogenous calnexin.

MTP / GBF1 ; 

PubMed: 26267806     


3T3-L1 cells, plated as above, were treated with brefeldin A (5 μg/ml, 3 hr), fixed and stained for MTP and GBF1. Brefeldin A treatment leads to redistribution of MTP from the juxtanuclear region, similar to what occurs with GBF1. Bars = 10 μm.

21868368 21576364 26267806
Growth inhibition assay
Cell viability; 

PubMed: 28462831     


Effects of brefeldin A on human prostate cancer cells. LNCaP and PC-3 cells were seeded in cell culture dishes and incubated for 24 h. The cells were then treated with different concentrations of brefeldin A for 72 h. The number of viable cells was determined by the trypan blue exclusion assay. Apoptosis was determined by propidium iodide staining and morphological assessment.

28462831

Protocol

Cell Research:

[5]

- Collapse
  • Cell lines: Human follicular lymphoma cell lines HF1A3, HF4.9 and HF28RA
  • Concentrations: 0 ng/mL -75 ng/mL
  • Incubation Time: 5 days
  • Method:

    HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 4 mg/mL (14.26 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 280.36
Formula

C16H24O4

CAS No. 20350-15-6
Storage powder
in solvent
Synonyms N/A
Smiles CC1CCCC=CC2CC(CC2C(C=CC(=O)O1)O)O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID