Rhodamine 123

Catalog No.S3577 Synonyms: RH-123, R-22420

For research use only.

Rhodamine 123 (RH-123, R-22420) is a fluorescent cationic dye used to label mitochondria in living cells. Rhodamine 123 inhibits ADP-stimulated respiration of mitochondria with Ki = 12 μM and ATPase activity of inverted inner membrane vesicles with Ki of 126 μM and partially purified F1-ATPase with Ki of 177 μM.

Rhodamine 123 Chemical Structure

CAS No. 62669-70-9

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Biological Activity

Description Rhodamine 123 (RH-123, R-22420) is a fluorescent cationic dye used to label mitochondria in living cells. Rhodamine 123 inhibits ADP-stimulated respiration of mitochondria with Ki = 12 μM and ATPase activity of inverted inner membrane vesicles with Ki of 126 μM and partially purified F1-ATPase with Ki of 177 μM.
Targets
ATPase [1]
(inverted inner membrane vesicles)
F1-ATPase [1]
(Cell-free assay)
126 μM(Ki) 177 μM(Ki)
In vitro

Knockout of ABCB1 by CRISPR/Cas9 system significantly enhances the sensitivity of ABCB1 substrate chemotherapeutic agents and the intracellular accumulation of rhodamine 123 and doxorubicin in MDR cancer cells.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: KBV200 and HCT-8/V cells with or without knockout of ABCB1
  • Concentrations: 10 μM
  • Incubation Time: 2 h
  • Method:

    The cell was seeded into a 6-well plate at a density of 2.5×105 cells/well. After adhered, the cells were then incubated with 10 μM rhodamine 123 or doxorubicin for 2 h at 37°C. After washing three times with ice-cold PBS, cells were analyzed with FCM.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 380.82
Formula
C21H17ClN2O3
CAS No. 62669-70-9
Storage 3 years -20°C powder
2 years -80°C in solvent

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