Saikosaponin D

For research use only.

Catalog No.S5454

Saikosaponin D Chemical Structure

Molecular Weight(MW): 780.98

Saikosaponin D, a calcium mobilizing agent (SERCA inhibitor), is a type of Saponin derivative, which is a component extracted from Bupleurum falactum. Saikosaponin D has anti-cancer activities.

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Biological Activity

Description Saikosaponin D, a calcium mobilizing agent (SERCA inhibitor), is a type of Saponin derivative, which is a component extracted from Bupleurum falactum. Saikosaponin D has anti-cancer activities.
In vitro

Saikosaponin D is a novel autophagic inducer that induces autophagy in various types of cancer cells through the formation of autophagosomes as measured by GFP-LC3 puncta formation. Saikosaponin D increases cytosolic calciumlevel via direct inhibition of sarcoplasmic/endoplasmic reticulum Ca2+ATPase pump, leading to autophagy induction through the activation of the Ca2+/calmodulin-dependent kinase kinase-AMP-activated protein kinase-mammalian target of rapamycin pathway. Its treatment causes the disruption of calcium homeostasis, which induces endoplasmic reticulum stress as well as the unfolded protein responses pathway[1]. Ssd can exhibit anti-cancer activities, which is mediated by the induction of apoptosis and autophagic cell death regardless of p53 status. Low dose Ssd is capable of sensitizing chremosensitive or chemoresistant lung and cervical cancer or hepatoma cells to anti-cancer drug- or radiation-induced apoptosis. Ssd also down-regulates PPM1D content, leading to the activation of Chk1, phosphorylation of Cdc25c and Cdk1, and subsequent G2/M arrest and apoptosis[2].


Cell Research:


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  • Cell lines: HeLa and MCF-7 cells
  • Concentrations: 10 μM
  • Incubation Time: 1, 4, 8 and 24 h
  • Method:

    HeLa and MCF-7 cells were treated with Ssd (10 μM) for the indicated time, whereas rapamycin (100 nM) and thapsigargin (1 μM) were used as positive control. Cell lysates were harvested and analyzed by western blot for ER stress markers.

    (Only for Reference)
Animal Research:


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  • Animal Models: Pathogen-free, BALB/C male mice aged 6-8 weeks old
  • Dosages: 1, 5, 10 and 20 mg/kg
  • Administration: intramuscularly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (128.04 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 780.98


CAS No. 20874-52-6
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID