Catalog No.S1241 Synonyms: Leurocristine
Molecular Weight(MW): 923.04
Vincristine sulfate is an inhibitor of polymerization of microtubules by binding to tubulin with IC50 of 32 μM in a cell-free assay.
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Plk inhibitor cooperates with chemotherapeutic agents. Conditional patched mutant tumor cells were treated with increasing concentrations of the chemotherapeutic agents vincristine alone or in combination with 10 nMol/L BI-2536. Cells were cultured for 48 hours, pulsed with 3H-Td, and harvested for analysis of 3H-Td incorporation at 66 hours.
Cancer Research, 2013, 73(20): 6310-22 . Vincristine sulfate purchased from Selleck.
Purity & Quality Control
Choose Selective Microtubule Associated Inhibitors
|Description||Vincristine sulfate is an inhibitor of polymerization of microtubules by binding to tubulin with IC50 of 32 μM in a cell-free assay.|
Vincristine inhibits net addition of tubulin dimers at assembly ends of steady-state microtubules with Ki of 85 nM.  At low concentrations, Vincristine stabilizes the spindle apparatus resulting in failure of the chromosomes to segregate leading to metaphase arrest and inhibition of mitosis. At higher concentrations, Vincristine may disrupt and induce total depolymerization of microtubules.  Vincristine induces apoptosis in tumor cells and inhibits SH-SY5Y cell proliferation with IC50 of 0.1 μM. Vincristine induces mitotic arrest and promots the expression of caspase-3 and -9 and cyclin B, while decreasing the expression of cyclin D.  Vincristine induced neurotoxicity is caused by interference with microtubule function, which results in blockage of axonal transport and thus in axonal degeneration. 
|In vivo||Vincristine (3 mg/kg) administrated by a single i.p. injection to mice bearing bilateral subcutaneous xenografts Rh12 or Rh18, induces mean growth delay of >120 and >52 day, and repopulating fractions of 0.06% and 5%, respectively.  Vincristine acts on subcutaneous colon 38 tumors in mice by host cell-mediated vascular effects as well as by direct tubulin-mediated cytotoxicity. Vincristine (5 mg/kg) reduces tumor blood flow of tumors by nearly 75% . |
-  Jordan MA, et al. Cancer Res, 1985, 45(6), 2741-2747.
-  Gidding CE. Crit Rev Oncol Hematol, 1999, 29(3), 267-287.
-  Tu Y, et al. Int J Mol Med, 2013, 31(1), 113-119.
|In vitro||DMSO||100 mg/mL (108.33 mM)|
|Water||60 mg/mL (65.0 mM)|
|In vivo||Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
For best results, use promptly after mixing.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
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Clinical Trial Information
|NCT Number||Recruitment||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03571321||Not yet recruiting||Acute Lymphoblastic Leukemia|ALL Childhood|ALL||University of Chicago|Incyte Corporation||September 5 2019||Phase 1|
|NCT03349281||Recruiting||Refractory Acute Lymphoblastic Leukemia|Relapsed Acute Lymphoblastic Leukemia||Julio Barredo|Takeda|University of Miami||March 2019||Phase 1|
|NCT03710772||Not yet recruiting||CD20 Positive|Mantle Cell Lymphoma||M.D. Anderson Cancer Center|National Cancer Institute (NCI)||January 31 2019||Phase 2|
|NCT03613428||Not yet recruiting||Acute T Cell Leukemia||Sichuan University||December 1 2018||Phase 1|Phase 2|
|NCT03742258||Not yet recruiting||Diffuse Large B-Cell Lymphoma|Diffuse Large B-Cell Lymphoma Activated B-Cell Type|Diffuse Large B-Cell Lymphoma Germinal Center B-Cell Type|Diffuse Large B-Cell Lymphoma Not Otherwise Specified|High Grade B-Cell Lymphoma With MYC and BCL2 and/or BCL6 Rearrangements|High Grade B-Cell Lymphoma Not Otherwise Specified|T-Cell/Histiocyte-Rich Large B-Cell Lymphoma||Northwestern University|National Cancer Institute (NCI)||December 29 2018||Phase 1|
|NCT03704714||Not yet recruiting||Aggressive Non-Hodgkin Lymphoma|B-Cell Non-Hodgkin Lymphoma|CD20 Positive|Diffuse Large B-Cell Lymphoma Unclassifiable|Intravascular Large B-Cell Lymphoma|Primary Mediastinal (Thymic) Large B-Cell Lymphoma|T-Cell/Histiocyte-Rich Large B-Cell Lymphoma|Transformed Follicular Lymphoma to Diffuse Large B-Cell Lymphoma||Northwestern University|Bristol-Myers Squibb (BMS)|National Cancer Institute (NCI)||November 8 2018||Phase 1|Phase 2|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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Frequently Asked Questions
I want to use the product #S1421 for mouse in vivo, could you please give us your advice about administration method?
According to the reference cited on our website, we test the solubility of S1421 Staurosporine in 4% DMSO/saline, and it is a suspension at 5 mg/mL. The compound can also be dissolved in the vehicle 4% DMSO/30% PEG 300/10% Tween 80/saline at 5 mg/ml as a clear solution for injection.