Vinorelbine Tartrate

Name: Vinorelbine Tartrate
Brand: Selleck Chemicals
SKU: S4269
Rating: 5 (16 reviews)

For research use only.

Catalog No.S4269

16 publications

CAS No. 125317-39-7

Vinorelbine Tartrate is a semi-synthetic vinca alkaloid, and inhibits mitosis through interaction with tubulin. Vinorelbine Tartrate exhibits anti-tumor activities via inducing the mitotic apoptosis, autophagy and inflammation.

Selleck's Vinorelbine Tartrate has been cited by 16 publications

Sci Rep, 2020, 10(1):13124

PubMed: 32753665 ( click the link to review the publication )

Cell Syst, 2019, 8(2):97-108

PubMed: 30797775 ( click the link to review the publication )

Cell Death Dis, 2019, 10(9):660

PubMed: 31506430 ( click the link to review the publication )

Cells, 2019, 8(8)

PubMed: 31390735 ( click the link to review the publication )

Mol Cancer Ther, 2019, 10.1158/1535-7163.MCT-18-1319

PubMed: 31594826 ( click the link to review the publication )

Transl Oncol, 2018, 11(6):1406-1418

PubMed: 30219696 ( click the link to review the publication )

Breast Cancer Res Treat, 2018, 172(1):23-32

PubMed: 30039287 ( click the link to review the publication )

Sci Adv, 2018, 4(9):eaar4666

PubMed: 30263952 ( click the link to review the publication )

Cell Chem Biol, 2017, 24(7):858-869

PubMed: 28669525 ( click the link to review the publication )

Lung Cancer, 2017, 109:1-8

PubMed: 28577937 ( click the link to review the publication )

J Biol Chem, 2017, 292(19):7806-7816

PubMed: 28320862 ( click the link to review the publication )

Mol Cancer Ther, 2016, 10.1158/1535-7163.MCT-16-0004

PubMed: 27256375 ( click the link to review the publication )

J Cancer Res Clin Oncol, 2016, 142(9):1955-66

PubMed: 27424189 ( click the link to review the publication )

PLoS One, 2015, 10(4):e0123901

PubMed: 25875914 ( click the link to review the publication )

Oncotarget, 2015, 6(29):27923-37

PubMed: 26314846 ( click the link to review the publication )

Oncotarget, 2015, 6(1):56-70

PubMed: 25474141 ( click the link to review the publication )

5 Customer Reviews

Activity of vinorelbine in NSCLC cell lines in cell viability assay. Activity of vinorelbine NCI-H23, NCI-H460, and NCC44 cell lines in a Cell Titer Glo cell viability assay. Cells were treated with increasing drug concentrations from 0-10000 nM. The data are plotted as the mean % of control cells against the corresponding drug concentration.

Cynthia Bernia from McGill University. Vinorelbine Tartrate purchased from Selleck.
JQ1 and chemotherapeutics (vinorelbine, docetaxel, cisplatin and carboplatin) cooperate to induce apoptosis. MDA-MB-231 and HS578T were exposed to drugs alone and in combination with JQ1, at the indicated doses. Apoptosis was examined after 72 hours of treatment by flow cytometry using Annexin V/ PI staining as previously described.

Mol Cancer Ther, 2016, 15(8):1823-33.. Vinorelbine Tartrate purchased from Selleck.
PyMT:Irs-2−/− cells transfected with empty vector (Irs2−/−) or IRS2 (Irs2−/−:IRS2) were treated with DMSO, 1 μm nocodazole, 20 nm vinblastine (Vin), or 20 nm vinorelbine (Vino) for 1 h and then stimulated with IGF-1 (10 ng/ml) for 15 min.

J Biol Chem, 2017, 292(19):7806-7816. Vinorelbine Tartrate purchased from Selleck.
Combination drug treatment increased multinucleated giant cells via spindle microtubule alteration and cell cycle arrest. Immunofluorescent staining of BT-20 cell line after treatment with vinorelbine (NVB) or vinorelbine in combination with 5 μM of either ME0328 (NVB + ME) or olaparib (NVB + Olaparib) for 24 h. Cells were stained with Dapi for DNA (blue). α-tubulin for microtubules (green) and the merge with a 100× objective. Abundant multinucleated giant cells observed in cells treated with NVB + ME and NVB + Olaparib comparing with NVB alone.

Breast Cancer Res Treat, 2018, 172(1):23-32. Vinorelbine Tartrate purchased from Selleck.
Dose-response curves of vinorelbine. Cells and microtissues are treated with increasing doses of the anti-mitotic microtubuli inhibitor vinorelbine for 24 and 48 h. The response of 2D cultivated cells compared to microtissues was determined measuring intra-cellular ATP, LDH release and cell viability (*p < 0.05; n = 3 for ATP assay; n = 3–7 for LDH assay; n = 3–4 for flow cytometry)

J Cancer Res Clin Oncol, 2016, 142(9):1955-66. Vinorelbine Tartrate purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description

Features

A semi-synthetic vinca alkaloid as an anti-mitotic chemotherapy drug.

Targets

Tubulin ^[1]
(Cell-free assay)

In vitro

Vinorelbine inhibits microtubule assembly by inducing tubulin aggregation into spirals and paracrystals. ^[1] Vinorelbine shows potent antiproliferative activity against a series of tumor cells, including human melanoma, non-small-cell lung cancer, breast cancer, etc. ^[2]^[3]^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
JEG3	NVy3OYhkS2WubDD2bYFjcWyrdImgZZN{[Xl?	NIPLT3oxNjBzLUGwNOKh\|ryP	NUXiPYFrPDhiaB?=	NWPkWm1[fmmwb4LlcIJqdmVic3nncolncWOjboTsfUBz\WS3Y3XkJGpGTzNiY3XscEB3cWGkaXzpeJkhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZItKGSxd36geI8h[SClb37j[Y51emG2aX;uJI9nKDBwMTFOwG0v	M1;RWFI6PDJ7OEmx
HTR-8/SVneo	MXLD[YxtKH[rYXLpcIl1gSCjc4PhfS=>	MUmwMlAyNTFyMNMg{txO	M2PFVlQ5KGh?	M1jhR5Zqdm:{ZXzibY5mKHOrZ37p[olk[W62bImgdoVlfWOnZDDIWHIuQC:\|dn7lc{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6ncjyg[I94diC2bzDhJINwdmOnboTyZZRqd25ib3[gNUDPxE1w	MXuyPVQzQTh7MR?=
EBC-1	NICzR4xHfW6ldHnvckBie3OjeR?=			NXTOOI0zTUKFLUGgZ4VtdHNiZHnzdIxigWWmIHGgcYFzc2WmbImgbIlocGW{IILheIUhd2ZiY3XscEBl\WG2aDD1dI9vKGOjcH3heIlvcWJidILlZZRu\W62LDDhcIJmcXRibn;0JJJm[WOqaX7nJFExOCVuIIfo[ZJm[XNidHjlJIVn\mWldDDpckBPS0lvSEG5PVMhf2G\|IHzhdodmdHlicnXzeJJq[3SnZDD0c{BqdmirYnn0bY9vKG:oIIDyc4xq\mW{YYTpc44>	NXzZbmxMOzB4N{S1NFI>
NCI-H1993	MoLZSpVv[3Srb36gZZN{[Xl?			MknGeIhmKHKjdHWgc4Yh[2WubDDk[YF1cCC3cH;uJINieG2jdHnubYIhfHKnYYTt[Y51KHejczDsZZJo\Wy7IILld5RzcWO2ZXSgeI8hcW6qaXLpeIlwdiCxZjDwdo9tcW[ncnH0bY9v	NH:wXlA{ODZ5NEWwNi=>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cyclin A / Cyclin B1 / p-Histone H3 / CDK1 / PCNA ; PubMed: 19017359 J Cell Mol Med When A498 and 786-O cells were treated with vinorelbine for 72 hrs and 48 hrs, respectively, increased Cyclin B1, phospho-histone H3, and Cdk1 were detected with doses of 100 nM and 10 nM of vinorelbine in A498 and 786-O tumours cells, respectively. In both cell lines, Cyclin A was decreased with a 1 μM dose. PCNA expression did not change with any treatments. In each case, β-Actin was used as a loading control.	19017359
Immunofluorescence	G3BP1 / eIF3b / eIF4G ; PubMed: 27083003 Oncotarget U2OS cells were stressed with sodium arsenite (SA, 100 μM), vinorelbine (VRB, 150 μM), vinblastine (VBL, 300 μM), vincristine (VCR, 750 μM) and paclitaxel (PCX, 400 μM) for 1 hour. Unstressed U2OS cells (no drug) were used as control. After treatment, cells were stained for SG markers G3BP1 (green), eIF4G (blue, shown as gray), eIF3b (red) and scored. Boxed region is shown enlarged with colors separated below each image; merged signals shown as gray. Size bar represents 10 μm. RPS6 / G3BP1 / TIAR ; PubMed: 27083003 Oncotarget VRB-induced SGs contain mRNAs and 40S ribosomal subunits. U2OS WT cells stably expressing ribosomal protein S6 (RPS6) fused to GFP (GFP-RPS6) were stressed with vinorelbine (VRB, 150 μM) or left untreated (no drug) for 1 hour. Upper panel: Cells were visualized for GFP-RPS6 (green) or SG markers G3BP1 (red) and TIAR (blue, shown as gray). Lower panel: Cells were stained with SG markers G3BP1 (green) and TIAR (blue, shown as gray). In situ hybridization with oligo-dT40 probe against polyadenylated mRNAs (red) was done as described. Boxed region is shown enlarged with colors separated below each image. Size bar represents 10 μm.	27083003
Growth inhibition assay	Cell number ; PubMed: 19017359 J Cell Mol Med Effect of vinorelbine on A498 and 786-O invasion in vitro. The effect of vinorelbine on A498 and 786-O cells invasion was assayed after 24 hrs of treatment. A 10 nM dose of vinorelbine was sufficient to inhibit both A498 and 786-O cells invasion after 6 hrs (P < 0.01 compared to untreated cells). The values represent an average of three separate experiments. **, P < 0.01 (treated group versus control group).	19017359

In vivo

In vivo, Vinorelbine also shows antitumour activity against a series of subcutaneously-implanted human tumour xenografts. ^[5]

Protocol

Animal Research:^[5]	- Collapse Animal Models: Bladder (BXF1299), pancreas (PAXF546), kidney (RXF944LX), colon (DLD-1, HT-29, TC37), central nervous system (SF-295), small cell lung (NCI-H69) and prostate (PC-3) xenografts. Dosages: ~10 mg/kg Administration: i.p. (Only for Reference)

References

- Collapse

Solubility (25°C)

In vitro	DMSO	100 mg/mL (92.66 mM)
	Water	100 mg/mL warmed (92.66 mM)
	Ethanol	'100 mg/mL warmed

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Vinorelbine Tartrate SDF

Molecular Weight	1079.11
Formula	C₄₅H₅₄N₄O₈.2C₄H₆O₆
CAS No.	125317-39-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CCC1=CC2CC(C3=C(CN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC.C(C(C(=O)O)O)(C(=O)O)O.C(C(C(=O)O)O)(C(=O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2020-09-01)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04299113	Recruiting	Drug: Vinorelbine\|Drug: Mocetinostat	Rhabdomyosarcoma	Jonsson Comprehensive Cancer Center\|Mirati Therapeutics Inc.\|Phase One Foundation	May 14 2020	Phase 1
NCT03891173	Recruiting	Drug: L-DOS47\|Drug: Cisplatin\|Drug: Vinorelbine	Lung Adenocarcinoma	Helix BioPharma Corporation\|KCR S.A.	February 19 2019	Phase 2
NCT02925000	Recruiting	Drug: TLC178	Cancer	Taiwan Liposome Company	June 19 2017	Phase 1\|Phase 2
NCT02658084	Terminated	Drug: Vinorelbine\|Drug: Trastuzumab Emtansine	Breast Cancer\|Metastatic Breast Cancer	University of Miami\|Genentech Inc.	April 12 2017	Phase 1\|Phase 2
NCT02768415	Active not recruiting	Drug: Apatinib\|Drug: Oral Vinorelbine	Breast Cancer	Chinese Academy of Medical Sciences	June 2016	Phase 2
NCT02619929	Completed	Drug: Vinorelbine oral	Non-Small-Cell Lung Cancer\|Breast Cancer	Pierre Fabre Pharma GmbH\|Winicker Norimed GmbH	February 2016	--

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Microtubule Associated Signaling Pathway Map

Related Microtubule Associated Products

TAI-1 ^New

TAI-1 is a potent and specific Hec1 inhibitor, which disrupts Hec1-Nek2 protein interaction.
Docetaxel Trihydrate ^New

Docetaxel (RP56976, NSC 628503), an analog of paclitaxel, is an inhibitor of depolymerisation of microtubules by binding to stabilized microtubules.
Combretastatin A4 ^New

Combretastatin A4 is a microtubule-targeting agent that binds β-tubulin with K_d of 0.4 μM.Phase 3.
Paclitaxel

Paclitaxel (NSC 125973, PTX, Taxol, Onxal) is a microtubule polymer stabilizer with IC50 of 0.1 pM in human endothelial cells.
Docetaxel

Docetaxel (RP56976, NSC 628503), an analog of paclitaxel, is an inhibitor of depolymerisation of microtubules by binding to stabilized microtubules.
Vincristine sulfate

Vincristine sulfate (Leurocristine) is an inhibitor of polymerization of microtubules by binding to tubulin with IC50 of 32 μM in a cell-free assay. Vincristine sulfate induces apoptosis.
Nocodazole

Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole induces apoptosis.
Cabazitaxel

Cabazitaxel (RPR-116258A, XRP6258, TXD 258) is a semi-synthetic derivative of a natural taxoid that kills cancer cells by inhibiting cell division and growth. Cabazitaxel exerts its effects by inhibiting microtubule growth and assembly, processes that are essential for cells to divide. Cabazitaxel induces autophagy via the PI3K/Akt/mTOR pathway.

Features:A semi-synthetic derivative of a natural taxoid.
Patupilone (Epothilone B)

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC_0.01 of 1.8 μM. Phase 2.

Choose Your Country or Region

By Signaling Pathways

By product type

Vinorelbine Tartrate