Vinorelbine Tartrate

Name: Vinorelbine Tartrate
Brand: Selleck Chemicals
SKU: S4269
Rating: 5 (20 reviews)

For research use only.

Catalog No.S4269

20 publications

CAS No. 125317-39-7

Vinorelbine Tartrate is a semi-synthetic vinca alkaloid, and inhibits mitosis through interaction with tubulin. Vinorelbine Tartrate exhibits anti-tumor activities via inducing the mitotic apoptosis, autophagy and inflammation.

Selleck's Vinorelbine Tartrate has been cited by 20 publications

Int J Mol Sci, 2020, 21(23)E9034

PubMed: 33261142 ( click the link to review the publication )

Cancer Chemother Pharmacol, 2020, 86(6):841-846

PubMed: 33070248 ( click the link to review the publication )

Sci Rep, 2020, 10(1):13124

PubMed: 32753665 ( click the link to review the publication )

Cell Syst, 2019, 8(2):97-108

PubMed: 30797775 ( click the link to review the publication )

Cell Death Dis, 2019, 10(9):660

PubMed: 31506430 ( click the link to review the publication )

Cells, 2019, 8(8)

PubMed: 31390735 ( click the link to review the publication )

Mol Cancer Ther, 2019, 10.1158/1535-7163.MCT-18-1319

PubMed: 31594826 ( click the link to review the publication )

Sci Rep, 2019, 9(1):19384

PubMed: 31852908 ( click the link to review the publication )

Transl Oncol, 2018, 11(6):1406-1418

PubMed: 30219696 ( click the link to review the publication )

Breast Cancer Res Treat, 2018, 172(1):23-32

PubMed: 30039287 ( click the link to review the publication )

Sci Adv, 2018, 4(9):eaar4666

PubMed: 30263952 ( click the link to review the publication )

Cell Chem Biol, 2017, 24(7):858-869

PubMed: 28669525 ( click the link to review the publication )

Lung Cancer, 2017, 109:1-8

PubMed: 28577937 ( click the link to review the publication )

J Biol Chem, 2017, 292(19):7806-7816

PubMed: 28320862 ( click the link to review the publication )

Mol Cancer Ther, 2016, 10.1158/1535-7163.MCT-16-0004

PubMed: 27256375 ( click the link to review the publication )

J Cancer Res Clin Oncol, 2016, 142(9):1955-66

PubMed: 27424189 ( click the link to review the publication )

Int J Cancer, 2015, 137(12):2947-58

PubMed: 26089022 ( click the link to review the publication )

PLoS One, 2015, 10(4):e0123901

PubMed: 25875914 ( click the link to review the publication )

Oncotarget, 2015, 6(29):27923-37

PubMed: 26314846 ( click the link to review the publication )

Oncotarget, 2015, 6(1):56-70

PubMed: 25474141 ( click the link to review the publication )

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description

Features

A semi-synthetic vinca alkaloid as an anti-mitotic chemotherapy drug.

Targets

Tubulin ^[1]
(Cell-free assay)

In vitro

Vinorelbine inhibits microtubule assembly by inducing tubulin aggregation into spirals and paracrystals. ^[1] Vinorelbine shows potent antiproliferative activity against a series of tumor cells, including human melanoma, non-small-cell lung cancer, breast cancer, etc. ^[2]^[3]^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
JEG3	M2PrNmNmdGxidnnhZoltcXS7IHHzd4F6	MXewMlAyNTFyMNMg{txO	NUCyd5F4PDhiaB?=	MkP1eolvd3KnbHLpcoUhe2mpbnnmbYNidnSueTDy[YR2[2WmIFrFS\|Mh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYKsJIRwf25idH:gZUBkd26lZX70doF1cW:wIH;mJFAvOSEQvF2u	NF7M[YEzQTR{OUi5NS=>
HTR-8/SVneo	NVzlTIpiS2WubDD2bYFjcWyrdImgZZN{[Xl?	NXfBSZRZOC5yMT2xNFDDqM7:TR?=	NWD3cnVOPDhiaB?=	Mo\yeolvd3KnbHLpcoUhe2mpbnnmbYNidnSueTDy[YR2[2WmIFjUVk05N3O4bnXvJINmdGxidnnhZoltcXS7IHnuJIEh\G:\|ZT3k[ZBmdmSnboSgcYFvdmW{LDDkc5dvKHSxIHGgZ49v[2WwdILheIlwdiCxZjCxJO69VS5?	NUHNbm5vOjl2Mkm4PVE>
EBC-1	NIfxS\|dHfW6ldHnvckBie3OjeR?=			NYmzVnpITUKFLUGgZ4VtdHNiZHnzdIxigWWmIHGgcYFzc2WmbImgbIlocGW{IILheIUhd2ZiY3XscEBl\WG2aDD1dI9vKGOjcH3heIlvcWJidILlZZRu\W62LDDhcIJmcXRibn;0JJJm[WOqaX7nJFExOCVuIIfo[ZJm[XNidHjlJIVn\mWldDDpckBPS0lvSEG5PVMhf2G\|IHzhdodmdHlicnXzeJJq[3SnZDD0c{BqdmirYnn0bY9vKG:oIIDyc4xq\mW{YYTpc44>	M4PRWFMxPjd2NUCy
NCI-H1993	MnvmSpVv[3Srb36gZZN{[Xl?			MlqzeIhmKHKjdHWgc4Yh[2WubDDk[YF1cCC3cH;uJINieG2jdHnubYIhfHKnYYTt[Y51KHejczDsZZJo\Wy7IILld5RzcWO2ZXSgeI8hcW6qaXLpeIlwdiCxZjDwdo9tcW[ncnH0bY9v	M{nSPVMxPjd2NUCy

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cyclin A / Cyclin B1 / p-Histone H3 / CDK1 / PCNA ; PubMed: 19017359 J Cell Mol Med When A498 and 786-O cells were treated with vinorelbine for 72 hrs and 48 hrs, respectively, increased Cyclin B1, phospho-histone H3, and Cdk1 were detected with doses of 100 nM and 10 nM of vinorelbine in A498 and 786-O tumours cells, respectively. In both cell lines, Cyclin A was decreased with a 1 μM dose. PCNA expression did not change with any treatments. In each case, β-Actin was used as a loading control.	19017359
Immunofluorescence	G3BP1 / eIF3b / eIF4G ; PubMed: 27083003 Oncotarget U2OS cells were stressed with sodium arsenite (SA, 100 μM), vinorelbine (VRB, 150 μM), vinblastine (VBL, 300 μM), vincristine (VCR, 750 μM) and paclitaxel (PCX, 400 μM) for 1 hour. Unstressed U2OS cells (no drug) were used as control. After treatment, cells were stained for SG markers G3BP1 (green), eIF4G (blue, shown as gray), eIF3b (red) and scored. Boxed region is shown enlarged with colors separated below each image; merged signals shown as gray. Size bar represents 10 μm. RPS6 / G3BP1 / TIAR ; PubMed: 27083003 Oncotarget VRB-induced SGs contain mRNAs and 40S ribosomal subunits. U2OS WT cells stably expressing ribosomal protein S6 (RPS6) fused to GFP (GFP-RPS6) were stressed with vinorelbine (VRB, 150 μM) or left untreated (no drug) for 1 hour. Upper panel: Cells were visualized for GFP-RPS6 (green) or SG markers G3BP1 (red) and TIAR (blue, shown as gray). Lower panel: Cells were stained with SG markers G3BP1 (green) and TIAR (blue, shown as gray). In situ hybridization with oligo-dT40 probe against polyadenylated mRNAs (red) was done as described. Boxed region is shown enlarged with colors separated below each image. Size bar represents 10 μm.	27083003
Growth inhibition assay	Cell number ; PubMed: 19017359 J Cell Mol Med Effect of vinorelbine on A498 and 786-O invasion in vitro. The effect of vinorelbine on A498 and 786-O cells invasion was assayed after 24 hrs of treatment. A 10 nM dose of vinorelbine was sufficient to inhibit both A498 and 786-O cells invasion after 6 hrs (P < 0.01 compared to untreated cells). The values represent an average of three separate experiments. **, P < 0.01 (treated group versus control group).	19017359

In vivo

In vivo, Vinorelbine also shows antitumour activity against a series of subcutaneously-implanted human tumour xenografts. ^[5]

Protocol

Animal Research:^[5]	- Collapse Animal Models: Bladder (BXF1299), pancreas (PAXF546), kidney (RXF944LX), colon (DLD-1, HT-29, TC37), central nervous system (SF-295), small cell lung (NCI-H69) and prostate (PC-3) xenografts. Dosages: ~10 mg/kg Administration: i.p. (Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	100 mg/mL (92.66 mM)
	Water	100 mg/mL warmed (92.66 mM)
	Ethanol	100 mg/mL warmed (92.66 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Vinorelbine Tartrate SDF

Molecular Weight	1079.11
Formula	C₄₅H₅₄N₄O₈.2C₄H₆O₆
CAS No.	125317-39-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CCC1=CC2CC(C3=C(CN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC.C(C(C(=O)O)O)(C(=O)O)O.C(C(C(=O)O)O)(C(=O)O)O

In vivo Formulation Calculator (Clear solution)

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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The Serial Dilution Calculator Equation

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Computed Result

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Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2020-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04299113	Recruiting	Drug: Vinorelbine\|Drug: Mocetinostat	Rhabdomyosarcoma	Jonsson Comprehensive Cancer Center\|Mirati Therapeutics Inc.\|Phase One Foundation	May 14 2020	Phase 1
NCT03891173	Recruiting	Drug: L-DOS47\|Drug: Cisplatin\|Drug: Vinorelbine	Lung Adenocarcinoma	Helix BioPharma Corporation\|KCR S.A.	February 19 2019	Phase 2
NCT02925000	Recruiting	Drug: TLC178	Cancer	Taiwan Liposome Company	June 19 2017	Phase 1\|Phase 2
NCT02658084	Terminated	Drug: Vinorelbine\|Drug: Trastuzumab Emtansine	Breast Cancer\|Metastatic Breast Cancer	University of Miami\|Genentech Inc.	April 12 2017	Phase 1\|Phase 2
NCT02768415	Active not recruiting	Drug: Apatinib\|Drug: Oral Vinorelbine	Breast Cancer	Chinese Academy of Medical Sciences	June 2016	Phase 2
NCT02619929	Completed	Drug: Vinorelbine oral	Non-Small-Cell Lung Cancer\|Breast Cancer	Pierre Fabre Pharma GmbH\|Winicker Norimed GmbH	February 2016	--

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Vinorelbine Tartrate