Vinorelbine ditartrate (KW-2307)

For research use only.

Catalog No.S4269 Synonyms: Nor-5'-anhydrovinblastine ditartrate

23 publications

Vinorelbine ditartrate (KW-2307) Chemical Structure

CAS No. 125317-39-7

Vinorelbine Tartrate (KW-2307, Nor-5'-anhydrovinblastine ditartrate) is a semi-synthetic vinca alkaloid, and inhibits mitosis through interaction with tubulin. Vinorelbine Tartrate exhibits anti-tumor activities via inducing the mitotic apoptosis, autophagy and inflammation.

Selleck's Vinorelbine ditartrate (KW-2307) has been cited by 23 publications

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Vinorelbine Tartrate (KW-2307, Nor-5'-anhydrovinblastine ditartrate) is a semi-synthetic vinca alkaloid, and inhibits mitosis through interaction with tubulin. Vinorelbine Tartrate exhibits anti-tumor activities via inducing the mitotic apoptosis, autophagy and inflammation.
Features A semi-synthetic vinca alkaloid as an anti-mitotic chemotherapy drug.
Targets
Tubulin [1]
(Cell-free assay)
In vitro

Vinorelbine inhibits microtubule assembly by inducing tubulin aggregation into spirals and paracrystals. [1] Vinorelbine shows potent antiproliferative activity against a series of tumor cells, including human melanoma, non-small-cell lung cancer, breast cancer, etc. [2][3][4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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DK-MG MmPsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXjk[XVZUW6qaXLpeIlwdiCxZjDoeY1idiCGSz3NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hPi52NEWxOkDPxE1w M2rNWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU2OzB{NT:nQnNCVkeHUkyvZV4>
NCI-H1693 MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVnJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMU[5N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hPy56OUW3NUDPxE1w MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1OVMxOjVxJ{7TRW5ITVJ:L3G+
JVM-2 M3jsWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml3yTY5pcWKrdHnvckBw\iCqdX3hckBLXk1vMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyIE2gPE4{QTd5OTFOwG0v NUPX[lRsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTV|MEK1M{c,W0GQR1XSQE9iRg>?
MSTO-211H NFLXelBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkfOTY5pcWKrdHnvckBw\iCqdX3hckBOW1SRLUKxNWgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDhwOECwOFIh|ryPLh?= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1OVMxOjVxJ{7TRW5ITVJ:L3G+
UACC-812 MnT6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1HKbGlvcGmkaYTpc44hd2ZiaIXtZY4hXUGFQz24NVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDhwOUGzOVUh|ryPLh?= NWO1V2JpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTV|MEK1M{c,W0GQR1XSQE9iRg>?
CW-2 MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX\EUoxQUW6qaXLpeIlwdiCxZjDoeY1idiCFVz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUA6NjN{NEm0JO69VS5? NV\uZnBmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTV|MEK1M{c,W0GQR1XSQE9iRg>?
NCI-H1155 M3n3fWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGLBSIFKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVE2PSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iOT62N|A5PSEQvF2u MkLmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVU{ODJ3Lze+V2FPT0WUPD;hQi=>
NCI-H720 NYjYcWh4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3;ONGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3NlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDFyLkm1OFIh|ryPLh?= MnSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVU{ODJ3Lze+V2FPT0WUPD;hQi=>
P31-FUJ M1\YUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEfJ[5dKdmirYnn0bY9vKG:oIHj1cYFvKFB|MT3GWWoh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDFzLkO4OVgh|ryPLh?= MkewQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVU{ODJ3Lze+V2FPT0WUPD;hQi=>
TALL-1 NVq2ZmZ5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIfNPI5KdmirYnn0bY9vKG:oIHj1cYFvKFSDTFytNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOTFwOEexOEDPxE1w NXXLU3prRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTV|MEK1M{c,W0GQR1XSQE9iRg>?
NCI-H1838 NFzrU4ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUPMe5FIUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE5OzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IEGyMlI{PDhizszNMi=> M{nEc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU2OzB{NT:nQnNCVkeHUkyvZV4>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cyclin A / Cyclin B1 / p-Histone H3 / CDK1 / PCNA ; 

PubMed: 19017359     


When A498 and 786-O cells were treated with vinorelbine for 72 hrs and 48 hrs, respectively, increased Cyclin B1, phospho-histone H3, and Cdk1 were detected with doses of 100 nM and 10 nM of vinorelbine in A498 and 786-O tumours cells, respectively. In both cell lines, Cyclin A was decreased with a 1 μM dose. PCNA expression did not change with any treatments. In each case, β-Actin was used as a loading control.

19017359
Immunofluorescence
RPS6 / G3BP1 / TIAR ; 

PubMed: 27083003     


VRB-induced SGs contain mRNAs and 40S ribosomal subunits. U2OS WT cells stably expressing ribosomal protein S6 (RPS6) fused to GFP (GFP-RPS6) were stressed with vinorelbine (VRB, 150 μM) or left untreated (no drug) for 1 hour. Upper panel: Cells were visualized for GFP-RPS6 (green) or SG markers G3BP1 (red) and TIAR (blue, shown as gray). Lower panel: Cells were stained with SG markers G3BP1 (green) and TIAR (blue, shown as gray). In situ hybridization with oligo-dT40 probe against polyadenylated mRNAs (red) was done as described. Boxed region is shown enlarged with colors separated below each image. Size bar represents 10 μm.

G3BP1 / eIF3b / eIF4G ; 

PubMed: 27083003     


U2OS cells were stressed with sodium arsenite (SA, 100 μM), vinorelbine (VRB, 150 μM), vinblastine (VBL, 300 μM), vincristine (VCR, 750 μM) and paclitaxel (PCX, 400 μM) for 1 hour. Unstressed U2OS cells (no drug) were used as control. After treatment, cells were stained for SG markers G3BP1 (green), eIF4G (blue, shown as gray), eIF3b (red) and scored. Boxed region is shown enlarged with colors separated below each image; merged signals shown as gray. Size bar represents 10 μm.

27083003
Growth inhibition assay
Cell number ; 

PubMed: 19017359     


Effect of vinorelbine on A498 and 786-O invasion in vitro. The effect of vinorelbine on A498 and 786-O cells invasion was assayed after 24 hrs of treatment. A 10 nM dose of vinorelbine was sufficient to inhibit both A498 and 786-O cells invasion after 6 hrs (P < 0.01 compared to untreated cells). The values represent an average of three separate experiments. **, P < 0.01 (treated group versus control group).

19017359
In vivo In vivo, Vinorelbine also shows antitumour activity against a series of subcutaneously-implanted human tumour xenografts. [5]

Protocol

Animal Research:[5]
- Collapse
  • Animal Models: Bladder (BXF1299), pancreas (PAXF546), kidney (RXF944LX), colon (DLD-1, HT-29, TC37), central nervous system (SF-295), small cell lung (NCI-H69) and prostate (PC-3) xenografts.
  • Dosages: ~10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (92.66 mM)
Water 100 mg/mL warmed (92.66 mM)
Ethanol ''100 mg/mL warmed

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1079.11
Formula

C45H54N4O8.2C4H6O6

CAS No. 125317-39-7
Storage powder
in solvent
Synonyms Nor-5'-anhydrovinblastine ditartrate
Smiles CCC1=CC2CC(C3=C(CN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC.C(C(C(=O)O)O)(C(=O)O)O.C(C(C(=O)O)O)(C(=O)O)O

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04896320 Not yet recruiting Drug: Tucatinib Breast Cancer Stage IV Providence Health & Services|Seagen Inc. July 2021 Phase 1|Phase 2
NCT04299113 Recruiting Drug: Vinorelbine|Drug: Mocetinostat Rhabdomyosarcoma Jonsson Comprehensive Cancer Center|Mirati Therapeutics Inc.|Phase One Foundation May 14 2020 Phase 1
NCT03891173 Terminated Drug: L-DOS47|Drug: Cisplatin|Drug: Vinorelbine Lung Adenocarcinoma Helix BioPharma Corporation|KCR S.A. February 19 2019 Phase 2
NCT02925000 Completed Drug: TLC178 Cancer Taiwan Liposome Company June 19 2017 Phase 1|Phase 2
NCT02658084 Terminated Drug: Vinorelbine|Drug: Trastuzumab Emtansine Breast Cancer|Metastatic Breast Cancer University of Miami|Genentech Inc. April 12 2017 Phase 1|Phase 2
NCT02768415 Unknown status Drug: Apatinib|Drug: Oral Vinorelbine Breast Cancer Chinese Academy of Medical Sciences June 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Microtubule Associated Signaling Pathway Map

Related Microtubule Associated Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID