Patupilone (EPO906, Epothilone B)

Catalog No.S1364

Patupilone (EPO906, Epothilone B) Chemical Structure

Molecular Weight(MW): 507.68

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.

Size Price Stock Quantity  
In DMSO USD 250 In stock
USD 70 In stock
USD 120 In stock
USD 210 In stock
USD 470 In stock
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3 Customer Reviews

  • Matrigel invasion assay of H1299 cells treated with SDF-1α or SDF-1α plus Epothilone B prior to 0 or 2 Gy irradiation. After the treatment for 12 h, the H1299 cells that migrated to the bottom surface of the membrane were stained with Giemsa, and the number of migrated cells was calculated manually.

    Oncotarget, 2015, 6(13):10893-907.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

  • Cells were seeded in 96 well paltes, and then treated with the indicated concentration of Epothilone B for 48 h. Cell survival was measured by a standarad MTT assay.

     

     

    Dr. Helen Sadik of Johns Hopkins University. Patupilone (EPO906, Epothilone B) purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.
Targets
Tubulin [1]
(Cell-free assay)
1.8 μM(EC0.01)
In vitro

Epothilone B shows better activity than Epothilone A. The EC0.01 of Epothilone B is 1.8 μM. Epothilone B potently inhibits cell proliferation in HCT116 cells, with IC50 of 0.8 nM. [1] Epothilone B induces mitotic arrest and displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 of 3 nM to 92 nM. Epothilone B competes with Taxol in binding to microtubules, with IC50 of 3.3 μM. [2] In MCF-7 cells overexpressing GFP-α-tubulin, Epothilone B (3.5 nM) efficiently blocks microtubule dynamics. Meanwhile, Epothilone B induces mitotic arrest with IC50 of 3.5 nM. [3] In multiple myeloma (MM) cells, including RPMI 8226, U266, MM.1S, LR5, and MR20, Epothilone B directly suppresses proliferation with IC50 of 1 nM to 10 nM. Similarly, Epothilone B (10 nM) also induces cell cycle arrest and apoptosis. [4] A recent study reveals that, in ovarian cancer Hey cells, Epothilone B (5 nM–100 nM) enhances surface epithelial cell adhesion antigen (EpCAM), without affecting the transcription or the total cellular level of EpCAM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LC-2-ad cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYq5eWduUW6qaXLpeIlwdiCxZjDoeY1idiCOQz2yMYFlKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy55N3WtNFYh|ryP Mk\CV2FPT0WU
human A431 cell NF;zT4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGHLdFhKdmirYnn0bY9vKG:oIHj1cYFvKEF2M{GgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25Mlg3\S1yNjFOwG0> MXPTRW5ITVJ?
human SW1710 cell NGrIUYdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{n4cWlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4yPGVvMEWg{txO NFrmN5VUSU6JRWK=
human Daoy cell NWnDeVJnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnPXTY5pcWKrdHnvckBw\iCqdX3hckBF[W:7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYpvwKwhUUN3ME2yMlc{\S1yNTFOwG0> MojlV2FPT0WU
human BFTC-905 cell NFS1WJpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoHOTY5pcWKrdHnvckBw\iCqdX3hckBDTlSFLUmwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvQTmnLUC1JO69VQ>? MoT3V2FPT0WU
human A375 cell NHLldVlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoLCTY5pcWKrdHnvckBw\iCqdX3hckBCOzd3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NXWtNFUh|ryP NYnRSHFUW0GQR1XS
human RKO cell NHXCcG5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M37vWGlvcGmkaYTpc44hd2ZiaIXtZY4hWkuRIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj60O4UuODVizszN M1H3RnNCVkeHUh?=
human KYSE-510 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUe4THh6UW6qaXLpeIlwdiCxZjDoeY1idiCNWWPFMVUyOCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwM{jlMVA2KM7:TR?= MVHTRW5ITVJ?
human HLE cell MoS5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mm\xTY5pcWKrdHnvckBw\iCqdX3hckBJVEViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16LkK2[U0xPSEQvF2= MoXJV2FPT0WU
human MCF7 cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVfJcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16Lk[2[U0xPSEQvF2= M3HW[3NCVkeHUh?=
human 786-0 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUXGPId5UW6qaXLpeIlwdiCxZjDoeY1idiB5OE[tNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTFibl2= NETOPJZUSU6JRWK=
human TE-8 cell NYLtTI1FT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFrw[YlKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlEyOSCwTR?= NUG1eGpCW0GQR1XS
ovarian carcinoma 1A9 cell M4Dhd2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{Pxd2lvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IH;2ZZJq[W5iY3HyZ4lvd22jIEHBPUBk\WyuIHfyc5d1cA>? NFvXVlgyPjF|NEmyPC=>
human PANC-03-27 cell NFTTOXVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYfJcohq[mm2aX;uJI9nKGi3bXHuJHBCVkNvMEOtNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF|MjFOwG0> M33F[HNCVkeHUh?=
human VMRC-RCZ cell MkjpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2TDdGlvcGmkaYTpc44hd2ZiaIXtZY4hXk2UQz3SR3oh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF|NjFOwG0> NImxPXJUSU6JRWK=
human IGROV-1 cell Mli2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGLsXI1KdmirYnn0bY9vKG:oIHj1cYFvKEmJUl;WMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF3NjFOwG0> MmXKV2FPT0WU
human MG-63 cell NFHD[oNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYLJcohq[mm2aX;uJI9nKGi3bXHuJG1INTZ|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOlUh|ryP MmiwV2FPT0WU
human SIG-M5 cell NXHjbnRFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVnJcohq[mm2aX;uJI9nKGi3bXHuJHNKTy2PNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVY6KM7:TR?= MV7TRW5ITVJ?
human NCI-H650 cell NFu2cWRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2LHVmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi2OVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF5IN88US=> NIi4cYJUSU6JRWK=
human PSN1 cell M4LIb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4LDe2lvcGmkaYTpc44hd2ZiaIXtZY4hWFOQMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVc{KM7:TR?= M1O0PXNCVkeHUh?=
human PA-1 cell NHn2[4JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4fiWmlvcGmkaYTpc44hd2ZiaIXtZY4hWEFvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVc1KM7:TR?= MoPXV2FPT0WU
KB-8511 cells M2Xt[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoXhO|IhcA>? MmrnR49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IITo[UBoem:5dHigc4YheGGlbHn0ZZhmdC2{ZYPpd5RidnRiaIXtZY4h\XCrZHXycY9q\CClYYLjbY5wdWFiY3XscJMhU0JvOEWxNUBjgSB3MDDw[ZJk\W62IDi3NkBpeiCneIDvd5Vz\SluIFnDOVA:OC5zODFOwG0> NHe5c3cyOTF|M{C4Oi=>
KB-31 cells NGfoO3lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnnQO|IhcA>? MWPDc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIHnubIljcXRidHjlJIdzd3e2aDDv[kBx[WOuaYThfIVtNXOnboPpeIl3\SCqdX3hckBmeGmmZYLtc4llKGOjcnPpco9u[SClZXzsd{BMSi1|MTDifUA2OCCyZYLj[Y51KCh5MjDodkBmgHCxc4Xy[Uk> NE\oRoYyOTF|M{C4Oi=>
human BHY cell NHG1XIJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4HiU2lvcGmkaYTpc44hd2ZiaIXtZY4hSki\IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xPVUhdk1? Mme0V2FPT0WU
human BB30-HNC cell NX3TXW1bT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUfnUWVuUW6qaXLpeIlwdiCxZjDoeY1idiCEQkOwMWhPSyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMkCxJO69VQ>? M3PP[nNCVkeHUh?=
human HOS cell MoHJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHPuSmdKdmirYnn0bY9vKG:oIHj1cYFvKEiRUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNlE2KM7:TR?= M{j6RnNCVkeHUh?=
human 639-V cell NXHkPXcyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF;DfGtKdmirYnn0bY9vKG:oIHj1cYFvKDZ|OT3WJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOThizszN M{jsXXNCVkeHUh?=
human ES1 cell  NXLifGhQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2P0[mlvcGmkaYTpc44hd2ZiaIXtZY4hTVNzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlEh|ryP NHq3OmlUSU6JRWK=
human TE-15 cell  M3HLc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEPoXFZKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUG1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOjVizszN NIfQVZdUSU6JRWK=
human LXF-289 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MknMTY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zPzdizszN MmPvV2FPT0WU
human CAL-12T cell M1fETWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkGwTY5pcWKrdHnvckBw\iCqdX3hckBESUxvMULUJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOzlizszN M2TFOXNCVkeHUh?=
human T84 cell MorUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3j5WWlvcGmkaYTpc44hd2ZiaIXtZY4hXDh2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yOEDPxE1? NEDwR4JUSU6JRWK=
human KYSE-450 cell Mn7tS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWfJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvNEWwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zPjdizszN M1;rXHNCVkeHUh?=
human LCLC-103H cell NInWTJhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mmr0TY5pcWKrdHnvckBw\iCqdX3hckBNS0yFLUGwN2gh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjNzIH7N MVzTRW5ITVJ?
human NCI-H810 cell NIXHO5JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MorJTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEixNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzJ{IN88US=> MWDTRW5ITVJ?
human HUTU-80 cell NVPQ[GRVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHi1dmpKdmirYnn0bY9vKG:oIHj1cYFvKEiXVGWtPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN{ODFOwG0> NIX1[FNUSU6JRWK=
human NCI-H460 cell M4HSeGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4nR[GlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi0OlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN|MzFOwG0> NFjVe2dUSU6JRWK=
human HGC-27 cell NIDJWoJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWnXNZhPUW6qaXLpeIlwdiCxZjDoeY1idiCKR1OtNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN|ODFOwG0> NFPpR29USU6JRWK=
human HSC-2 cell NX7iZ5VpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFPDVFBKdmirYnn0bY9vKG:oIHj1cYFvKEiVQz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{PjNibl2= M3LyUnNCVkeHUh?=
human IA-LM cell MXnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUjJcohq[mm2aX;uJI9nKGi3bXHuJGlCNUyPIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6zPVMh|ryP M2XBVHNCVkeHUh?=
human HMV-II cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIK3ZY1KdmirYnn0bY9vKG:oIHj1cYFvKEiPVj3JTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzl2IN88US=> M2Pac3NCVkeHUh?=
human COLO-679 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1TR[WlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz22O|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN7NDFOwG0> M{G1bXNCVkeHUh?=
human NCI-H2122 cell NUnIN2YxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mk\xTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKxNlIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjRzIN88US=> M32xTnNCVkeHUh?=
human CAL-39 cell M4\ub2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmTQTY5pcWKrdHnvckBw\iCqdX3hckBESUxvM{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQyOSEQvF2= MWTTRW5ITVJ?
human SW954 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX30WJR2UW6qaXLpeIlwdiCxZjDoeY1idiCVV{m1OEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPDN4IN88US=> NHXnOmxUSU6JRWK=
human LCLC-97TM1 cell NFSwdFBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmK4TY5pcWKrdHnvckBw\iCqdX3hckBNS0yFLUm3WG0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC52NESg{txO MVvTRW5ITVJ?
human KYSE-270 cell MofMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlziTY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUK3NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPDR3IN88US=> M{j0[nNCVkeHUh?=
human NB13 cell M2PibWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{j5XWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOFQ4KM7:TR?= M{S4d3NCVkeHUh?=
human SK-LMS-1 cell NELKO5hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUPxempOUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MUXMuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNkS2JO69VQ>? M1jTWnNCVkeHUh?=
human SW620 cell MkDOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHTFZ5RKdmirYnn0bY9vKG:oIHj1cYFvKFOZNkKwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE43PDhizszN NVzUcot{W0GQR1XS
human HCT-15 cell MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXfJcohq[mm2aX;uJI9nKGi3bXHuJGhEXC1zNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|E4KM7:TR?= M13ZdXNCVkeHUh?=
human DU-145 cell NYO5bnZwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIfORo5KdmirYnn0bY9vKG:oIHj1cYFvKESXLUG0OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPzJzIN88US=> Ml[xV2FPT0WU
human ST486 cell M4HmOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWLo[YlOUW6qaXLpeIlwdiCxZjDoeY1idiCVVES4OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPzJzIN88US=> MV7TRW5ITVJ?
human A427 cell MoDVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXHJcohq[mm2aX;uJI9nKGi3bXHuJGE1OjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkiyPEDPxE1? NX:ySmE1W0GQR1XS
human HT-1080 cell M2qyXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVntVJZKUW6qaXLpeIlwdiCxZjDoeY1idiCKVD2xNFgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56NEKg{txO MUPTRW5ITVJ?
human NCI-H2009 cell M4r0UWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml;nTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKwNFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh4MjFOwG0> Ml;wV2FPT0WU
human SK-LU-1 cell M1GyTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFu3R4pKdmirYnn0bY9vKG:oIHj1cYFvKFONLVzVMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh4NjFOwG0> MUjTRW5ITVJ?
human SCC-4 cell Ml;0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF;veIpKdmirYnn0bY9vKG:oIHj1cYFvKFOFQz20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PzdizszN MofEV2FPT0WU
human NCI-H1299 cell M3fEemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIq1do5KdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVI6QSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwOEig{txO MlrvV2FPT0WU
human NH-12 cell NIC3bYZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGLiWGZKdmirYnn0bY9vKG:oIHj1cYFvKE6KLUGyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45QTFizszN NEWwSZhUSU6JRWK=
human SK-UT-1 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYH4WIZJUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3VWE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56OUKg{txO MYnTRW5ITVJ?
human Bel7402 Mk\pVJJwdGmoZYLheIlwdiCjc4PhfS=> NF;XbJA4OiCq NHjVPGVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLlcFc1ODJiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmeiCJbH:gZZN{[XluIFnDOVA:OC57IN88US=> M4r5NFIzOzJyM{W0
human A388 cell M3K0VWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEL6PGhKdmirYnn0bY9vKG:oIHj1cYFvKEF|OEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlk1KM7:TR?= NH\BfIxUSU6JRWK=
human SW982 cell MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkPrTY5pcWKrdHnvckBw\iCqdX3hckBUXzl6MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPVk6KM7:TR?= M2TGPXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo In a mouse xenograft model of RPMI 8226 cells, Epothilone B (2.5 mg/kg–4 mg/kg) prolongs survival and suppresses tumor growth. [4] Similarly, in mouse xenograft models of prostate cancer cells, including DU145 and PC3, Epothilone B at the same dose also inhibits tumor growth. [6]

Protocol

Kinase Assay:[1]
+ Expand

Tubulin polymerization assay:

Calf brain microtubule proteins (MTP) are purified, which includes approximately 15%–20% microtubule associated proteins. The buffer (MES buffer) used for the Epothilone B-microtubule studies contains 0.1 M 2-morpholinoethanesulfonic acid (MES), 1 mM EGTA, 0.5 mM MgCl2, and 3 M glycerol at pH 6.6. Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone B is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope and expressed as the mean with standard deviation obtained from three different concentrations.
Cell Research:[2]
+ Expand
  • Cell lines: KB3-1, KBV-1, Hela, and Hs578T cells
  • Concentrations: 0–1 μM
  • Incubation Time: 72 hours
  • Method: For mitotic block and aberrant mitosis, cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips. After 24 hours, cells are treated with Epothilone B and scored at regular intervals. For the cytotoxicity analysis, cells are counted and scored as trypan blue positive or negative. Concurrently, coverslips andaliquots of cells in the culture supernatant are fixed and stained with Hoechst33342 in PBS. These cells are scored for cells blocked at the G2/M transition and aberrant mitosis.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Mice xenograft model of RPMI 8226 cells
  • Formulation: Dissolved in30% PEG-300
  • Dosages: 2.5 mg/kg–4 mg/kg
  • Administration: Inject intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 102 mg/mL (200.91 mM)
Ethanol 102 mg/mL (200.91 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.68
Formula

C27H41NO6S

CAS No. 152044-54-7
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00715013 Completed Recurrent Glioblastoma Planned for Reoperation University of Zurich July 2008 Phase 1|Phase 2
NCT00496600 Completed Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers The State University of New Jersey July 2007 Phase 1
NCT00442741 Withdrawn Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1
NCT00468260 Terminated Advanced Malignancies Novartis Pharmaceuticals|Novartis May 2007 Phase 1
NCT00448396 Completed Advanced Malignancies Novartis Pharmaceuticals|Novartis March 2007 Phase 1
NCT00407251 Completed Hormone Refractory Prostate Cancer British Columbia Cancer Agency February 2007 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Microtubule Associated Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID