Patupilone (EPO906, Epothilone B)

Catalog No.S1364

Patupilone (EPO906, Epothilone B) Chemical Structure

Molecular Weight(MW): 507.68

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.

Size Price Stock Quantity  
In DMSO USD 250 In stock
USD 70 In stock
USD 120 In stock
USD 210 In stock
USD 470 In stock
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Cited by 7 Publications

4 Customer Reviews

  • Matrigel invasion assay of H1299 cells treated with SDF-1α or SDF-1α plus Epothilone B prior to 0 or 2 Gy irradiation. After the treatment for 12 h, the H1299 cells that migrated to the bottom surface of the membrane were stained with Giemsa, and the number of migrated cells was calculated manually.

    Oncotarget, 2015, 6(13):10893-907.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

  • Cells were seeded in 96 well paltes, and then treated with the indicated concentration of Epothilone B for 48 h. Cell survival was measured by a standarad MTT assay.

     

     

    Dr. Helen Sadik of Johns Hopkins University. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9. Patupilone (EPO906, Epothilone B) purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.
Targets
Tubulin [1]
(Cell-free assay)
1.8 μM(EC0.01)
In vitro

Epothilone B shows better activity than Epothilone A. The EC0.01 of Epothilone B is 1.8 μM. Epothilone B potently inhibits cell proliferation in HCT116 cells, with IC50 of 0.8 nM. [1] Epothilone B induces mitotic arrest and displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 of 3 nM to 92 nM. Epothilone B competes with Taxol in binding to microtubules, with IC50 of 3.3 μM. [2] In MCF-7 cells overexpressing GFP-α-tubulin, Epothilone B (3.5 nM) efficiently blocks microtubule dynamics. Meanwhile, Epothilone B induces mitotic arrest with IC50 of 3.5 nM. [3] In multiple myeloma (MM) cells, including RPMI 8226, U266, MM.1S, LR5, and MR20, Epothilone B directly suppresses proliferation with IC50 of 1 nM to 10 nM. Similarly, Epothilone B (10 nM) also induces cell cycle arrest and apoptosis. [4] A recent study reveals that, in ovarian cancer Hey cells, Epothilone B (5 nM–100 nM) enhances surface epithelial cell adhesion antigen (EpCAM), without affecting the transcription or the total cellular level of EpCAM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LC-2-ad cell MnTwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVL1V5BXUW6qaXLpeIlwdiCxZjDoeY1idiCOQz2yMYFlKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy55N3WtNFYh|ryP NXXLW4xHW0GQR1XS
human A431 cell Mo\QS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUfJcohq[mm2aX;uJI9nKGi3bXHuJGE1OzFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD17Lki2[U0xPiEQvF2= NEPvOoNUSU6JRWK=
human SW1710 cell NY[0UYd6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYrGUYt1UW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjF2ZT2wOUDPxE1? MoPKV2FPT0WU
human Daoy cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUDJcohq[mm2aX;uJI9nKGi3bXHuJGRid3liY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3Ojef-8kEBKSzVyPUKuO|NmNTB3IN88US=> MYnTRW5ITVJ?
human BFTC-905 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXf2NnpXUW6qaXLpeIlwdiCxZjDoeY1idiCERmTDMVkxPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwOUnlMVA2KM7:TR?= MmDKV2FPT0WU
human A375 cell MmHDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXzZRWFmUW6qaXLpeIlwdiCxZjDoeY1idiCDM{e1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OYUuODVizszN M{LtXXNCVkeHUh?=
human RKO cell NGrQ[|BIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml;jTY5pcWKrdHnvckBw\iCqdX3hckBTU09iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkS3[U0xPSEQvF2= NYWyeGRqW0GQR1XS
human KYSE-510 cell NF7C[JRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn\zTY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUWxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvOzinLUC1JO69VQ>? NWrPcI9zW0GQR1XS
human HLE cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFnkbXdKdmirYnn0bY9vKG:oIHj1cYFvKEiORTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlZmNTB3IN88US=> Ml3XV2FPT0WU
human MCF7 cell M3GwNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHTVZ3ZKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlY3\S1yNTFOwG0> MUDTRW5ITVJ?
human 786-0 cell NWHjXFlGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn2xTY5pcWKrdHnvckBw\iCqdX3hckA4QDZvMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVEhdk1? NEKyW2xUSU6JRWK=
human TE-8 cell NUDkSFNPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M16xdGlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVEyKG6P M3PLXnNCVkeHUh?=
ovarian carcinoma 1A9 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUnJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCxdnHybYFvKGOjcnPpco9u[SBzQUmgZ4VtdCCpcn;3eIg> MlvFNVYyOzR7Mki=
human PANC-03-27 cell NXHE[XdGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFXWN|JKdmirYnn0bY9vKG:oIHj1cYFvKFCDTlOtNFMuOjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkGzNkDPxE1? M3HPR3NCVkeHUh?=
human VMRC-RCZ cell NHvt[GxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFTlb3dKdmirYnn0bY9vKG:oIHj1cYFvKF[PUlOtVmNbKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zM{[g{txO NWPFSIlnW0GQR1XS
human IGROV-1 cell NX3mPIY3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKGi3bXHuJGlIWk:YLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE2PiEQvF2= NXvEeWJMW0GQR1XS
human MG-63 cell M2Pyfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mny5TY5pcWKrdHnvckBw\iCqdX3hckBOTy14MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVY2KM7:TR?= MVnTRW5ITVJ?
human SIG-M5 cell NXW5NppmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkT6TY5pcWKrdHnvckBw\iCqdX3hckBUUUdvTUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE3QSEQvF2= M{jkcXNCVkeHUh?=
human NCI-H650 cell NWDTS4xsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVribFZHUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFY2OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMUeg{txO NXTsTWZIW0GQR1XS
human PSN1 cell M4HRWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{X6WWlvcGmkaYTpc44hd2ZiaIXtZY4hWFOQMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVc{KM7:TR?= NWjWRVBWW0GQR1XS
human PA-1 cell Ml7FS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NU\zOpZLUW6qaXLpeIlwdiCxZjDoeY1idiCSQT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPzRizszN MWfTRW5ITVJ?
KB-8511 cells NHi2SFZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXvZdYN6PzJiaB?= M{DidWNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDD0bIUh\3Kxd4ToJI9nKHCjY3zpeIF5\WxvcnXzbZN1[W62IHj1cYFvKGWyaXTldo1wcWRiY3HyZ4lvd22jIHPlcIx{KEuELUi1NVEh[nliNUCgdIVz[2WwdDCoO|IhcHJiZYjwc5N2emVrLDDJR|UxRTBwMUig{txO NHrUeY0yOTF|M{C4Oi=>
KB-31 cells M2PDR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF33eXo4OiCq NUOxTJlCS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JJRp\SCpcn;3eIghd2ZicHHjcIl1[XinbD3z[Y5{cXSrdnWgbJVu[W5iZYDp[IVzdW:rZDDjZZJkcW6xbXGgZ4VtdHNiS1KtN|Eh[nliNUCgdIVz[2WwdDCoO|IhcHJiZYjwc5N2emVr M33DOFEyOTN|MEi2
human BHY cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIKzNJdKdmirYnn0bY9vKG:oIHj1cYFvKEKKWTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVk2KG6P MX7TRW5ITVJ?
human BB30-HNC cell NHzqVFlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX7rZ49{UW6qaXLpeIlwdiCxZjDoeY1idiCEQkOwMWhPSyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMkCxJO69VQ>? M{\4ZnNCVkeHUh?=
human HOS cell Mn:zS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUPnRWpxUW6qaXLpeIlwdiCxZjDoeY1idiCKT2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIyPSEQvF2= M4TQ[XNCVkeHUh?=
human 639-V cell NX;ofVU2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYLkdnZwUW6qaXLpeIlwdiCxZjDoeY1idiB4M{mtWkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjF6IN88US=> M{fhXHNCVkeHUh?=
human ES1 cell  NGPZOWpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1XGOGlvcGmkaYTpc44hd2ZiaIXtZY4hTVNzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlEh|ryP MVLTRW5ITVJ?
human TE-15 cell  M4C4Wmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MY\Jcohq[mm2aX;uJI9nKGi3bXHuJHRGNTF3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlUh|ryP M3:xNHNCVkeHUh?=
human LXF-289 cell M4m2Tmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXLOU4NqUW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5{N{eg{txO NV3ucopxW0GQR1XS
human CAL-12T cell NHu5dWtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnTSTY5pcWKrdHnvckBw\iCqdX3hckBESUxvMULUJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOzlizszN NVPHb4QyW0GQR1XS
human T84 cell NU[1[4VzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlOxTY5pcWKrdHnvckBw\iCqdX3hckBVQDRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkK0JO69VQ>? MUHTRW5ITVJ?
human KYSE-450 cell M{T6dmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnPZTY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUS1NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjZ5IN88US=> MVHTRW5ITVJ?
human LCLC-103H cell NGHMXlRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnT3TY5pcWKrdHnvckBw\iCqdX3hckBNS0yFLUGwN2gh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjNzIH7N MWnTRW5ITVJ?
human NCI-H810 cell NIi5dmRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mkn0TY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEixNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzJ{IN88US=> M{\vZ3NCVkeHUh?=
human HUTU-80 cell NH\0UVdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUXJcohq[mm2aX;uJI9nKGi3bXHuJGhWXFVvOECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlMzQCEQvF2= M3ToN3NCVkeHUh?=
human NCI-H460 cell NFXvPGpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MonGTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSES2NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzN|IN88US=> NI\iPJRUSU6JRWK=
human HGC-27 cell M37FUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlPWTY5pcWKrdHnvckBw\iCqdX3hckBJT0NvMkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM{QCEQvF2= MYXTRW5ITVJ?
human HSC-2 cell M{XjS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{\4NWlvcGmkaYTpc44hd2ZiaIXtZY4hUFOFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM3OyCwTR?= NWXlVYRIW0GQR1XS
human IA-LM cell M2LaTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXTOV|dKUW6qaXLpeIlwdiCxZjDoeY1idiCLQT3MUUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzl|IN88US=> MkXwV2FPT0WU
human HMV-II cell NYDLfnh[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVnVXHgzUW6qaXLpeIlwdiCxZjDoeY1idiCKTW[tTWkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN7NDFOwG0> MljCV2FPT0WU
human COLO-679 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH3adJZKdmirYnn0bY9vKG:oIHj1cYFvKEORTF:tOlc6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5|OUSg{txO NHnybGRUSU6JRWK=
human NCI-H2122 cell NXXrdVF[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkPCTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKxNlIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjRzIN88US=> NVXHToIxW0GQR1XS
human CAL-39 cell NG\1O4VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXXJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOFEyKM7:TR?= M4rXWnNCVkeHUh?=
human SW954 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mme5TY5pcWKrdHnvckBw\iCqdX3hckBUXzl3NDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOFM3KM7:TR?= NVTaPWxOW0GQR1XS
human LCLC-97TM1 cell M4PtVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXTJcohq[mm2aX;uJI9nKGi3bXHuJGxEVENvOUfUUVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjR2NDFOwG0> NEnpPZBUSU6JRWK=
human KYSE-270 cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlvITY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUK3NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPDR3IN88US=> NFHvTphUSU6JRWK=
human NB13 cell NEn5O4NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn\PTY5pcWKrdHnvckBw\iCqdX3hckBPSjF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD60OFch|ryP M1rLXnNCVkeHUh?=
human SK-LMS-1 cell MmWyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEHKV3BKdmirYnn0bY9vKG:oIHj1cYFvKFONLVzNV{0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC54NE[g{txO NGe1fGNUSU6JRWK=
human SW620 cell M2jMSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1rSSmlvcGmkaYTpc44hd2ZiaIXtZY4hW1d4MkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlY1QCEQvF2= MW\TRW5ITVJ?
human HCT-15 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4fWdGlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWLUG1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE44OTdizszN M3;GdnNCVkeHUh?=
human DU-145 cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mki0TY5pcWKrdHnvckBw\iCqdX3hckBFXS1zNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlczOSEQvF2= MVrTRW5ITVJ?
human ST486 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3PoOGlvcGmkaYTpc44hd2ZiaIXtZY4hW1R2OE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlczOSEQvF2= M1P3UXNCVkeHUh?=
human A427 cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIPzPHZKdmirYnn0bY9vKG:oIHj1cYFvKEF2MkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlgzQCEQvF2= M{S0NnNCVkeHUh?=
human HT-1080 cell NGPWfm1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1P1VmlvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMUC4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDR{IN88US=> MnrBV2FPT0WU
human NCI-H2009 cell NWL1NGE2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn;yTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKwNFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh4MjFOwG0> MXTTRW5ITVJ?
human SK-LU-1 cell NUO0VWlPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIjKO3VKdmirYnn0bY9vKG:oIHj1cYFvKFONLVzVMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh4NjFOwG0> MX;TRW5ITVJ?
human SCC-4 cell NWj5[Vc{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmPXTY5pcWKrdHnvckBw\iCqdX3hckBUS0NvNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPFc4KM7:TR?= MnzvV2FPT0WU
human NCI-H1299 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmHSTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEGyPVkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh6IN88US=> MnfyV2FPT0WU
human NH-12 cell M{XlOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVTw[VNTUW6qaXLpeIlwdiCxZjDoeY1idiCQSD2xNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDlzIN88US=> M2fCb3NCVkeHUh?=
human SK-UT-1 cell NXvOO5BoT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXfsPXdoUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3VWE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56OUKg{txO Mn3qV2FPT0WU
human Bel7402 M2XSSHBzd2yrZnXyZZRqd25iYYPzZZk> M3XzOlczKGh? MnrRRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEZXy3OFAzKGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIhT2yxIHHzd4F6NCCLQ{WwQVAvQSEQvF2= NYPjU5cxOjJ|MkCzOVQ>
human A388 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGE{QDhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkm0JO69VQ>? M3X0cXNCVkeHUh?=
human SW982 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUTJcohq[mm2aX;uJI9nKGi3bXHuJHNYQTh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD65PVkh|ryP NEnHT2FUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo In a mouse xenograft model of RPMI 8226 cells, Epothilone B (2.5 mg/kg–4 mg/kg) prolongs survival and suppresses tumor growth. [4] Similarly, in mouse xenograft models of prostate cancer cells, including DU145 and PC3, Epothilone B at the same dose also inhibits tumor growth. [6]

Protocol

Kinase Assay:[1]
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Tubulin polymerization assay:

Calf brain microtubule proteins (MTP) are purified, which includes approximately 15%–20% microtubule associated proteins. The buffer (MES buffer) used for the Epothilone B-microtubule studies contains 0.1 M 2-morpholinoethanesulfonic acid (MES), 1 mM EGTA, 0.5 mM MgCl2, and 3 M glycerol at pH 6.6. Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone B is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope and expressed as the mean with standard deviation obtained from three different concentrations.
Cell Research:[2]
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  • Cell lines: KB3-1, KBV-1, Hela, and Hs578T cells
  • Concentrations: 0–1 μM
  • Incubation Time: 72 hours
  • Method: For mitotic block and aberrant mitosis, cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips. After 24 hours, cells are treated with Epothilone B and scored at regular intervals. For the cytotoxicity analysis, cells are counted and scored as trypan blue positive or negative. Concurrently, coverslips andaliquots of cells in the culture supernatant are fixed and stained with Hoechst33342 in PBS. These cells are scored for cells blocked at the G2/M transition and aberrant mitosis.
    (Only for Reference)
Animal Research:[4]
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  • Animal Models: Mice xenograft model of RPMI 8226 cells
  • Formulation: Dissolved in30% PEG-300
  • Dosages: 2.5 mg/kg–4 mg/kg
  • Administration: Inject intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 102 mg/mL (200.91 mM)
Ethanol 102 mg/mL (200.91 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.68
Formula

C27H41NO6S

CAS No. 152044-54-7
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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Molecular Weight Calculator

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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00496600 Completed Drug: Patupilone|Drug: RAD001 Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers The State University of New Jersey July 2007 Phase 1
NCT00442741 Withdrawn Drug: Patupilone|Drug: Patupilone + Omeprazole Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1
NCT00468260 Terminated Drug: Patupilone Advanced Malignancies Novartis Pharmaceuticals|Novartis May 2007 Phase 1
NCT00448396 Completed Drug: Patupilone Advanced Malignancies Novartis Pharmaceuticals|Novartis March 2007 Phase 1
NCT00426140 Completed Drug: Patupilone Advanced Malignancies|Solid Tumors Novartis Pharmaceuticals|Novartis August 2006 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Microtubule Associated Signaling Pathway Map

Related Microtubule Associated Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID