Patupilone (Epothilone B)

For research use only.

Catalog No.S1364 Synonyms: EPO906

8 publications

Patupilone (Epothilone B) Chemical Structure

CAS No. 152044-54-7

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.

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Selleck's Patupilone (Epothilone B) has been cited by 8 publications

4 Customer Reviews

  • Matrigel invasion assay of H1299 cells treated with SDF-1α or SDF-1α plus Epothilone B prior to 0 or 2 Gy irradiation. After the treatment for 12 h, the H1299 cells that migrated to the bottom surface of the membrane were stained with Giemsa, and the number of migrated cells was calculated manually.

    Oncotarget, 2015, 6(13):10893-907.. Patupilone (Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9.. Patupilone (Epothilone B) purchased from Selleck.

  • Cells were seeded in 96 well paltes, and then treated with the indicated concentration of Epothilone B for 48 h. Cell survival was measured by a standarad MTT assay.

     

     

    Dr. Helen Sadik of Johns Hopkins University. Patupilone (Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9. Patupilone (Epothilone B) purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.
Targets
Tubulin [1]
(Cell-free assay)
1.8 μM(EC0.01)
In vitro

Epothilone B shows better activity than Epothilone A. The EC0.01 of Epothilone B is 1.8 μM. Epothilone B potently inhibits cell proliferation in HCT116 cells, with IC50 of 0.8 nM. [1] Epothilone B induces mitotic arrest and displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 of 3 nM to 92 nM. Epothilone B competes with Taxol in binding to microtubules, with IC50 of 3.3 μM. [2] In MCF-7 cells overexpressing GFP-α-tubulin, Epothilone B (3.5 nM) efficiently blocks microtubule dynamics. Meanwhile, Epothilone B induces mitotic arrest with IC50 of 3.5 nM. [3] In multiple myeloma (MM) cells, including RPMI 8226, U266, MM.1S, LR5, and MR20, Epothilone B directly suppresses proliferation with IC50 of 1 nM to 10 nM. Similarly, Epothilone B (10 nM) also induces cell cycle arrest and apoptosis. [4] A recent study reveals that, in ovarian cancer Hey cells, Epothilone B (5 nM–100 nM) enhances surface epithelial cell adhesion antigen (EpCAM), without affecting the transcription or the total cellular level of EpCAM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LC-2-ad cell NHLZR4tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXTJcohq[mm2aX;uJI9nKGi3bXHuJGxENTJvYXSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlc4\S1yNjFOwG0> NVfHT|RuW0GQR1XS
human A431 cell NVHaTGk6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVTYOZJnUW6qaXLpeIlwdiCxZjDoeY1idiCDNEOxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU45PmVvME[g{txO M3viVnNCVkeHUh?=
human SW1710 cell NXLLSWRkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIqybpJKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOTSnLUC1JO69VQ>? MWPTRW5ITVJ?
human Daoy cell MnzlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF7ZfXBKdmirYnn0bY9vKG:oIHj1cYFvKESjb4mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVIvPzOnLUC1JO69VQ>? NFXjTHdUSU6JRWK=
human BFTC-905 cell MlO0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVf5bHhZUW6qaXLpeIlwdiCxZjDoeY1idiCERmTDMVkxPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwOUnlMVA2KM7:TR?= NXPHSlZMW0GQR1XS
human A375 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mo\ZTY5pcWKrdHnvckBw\iCqdX3hckBCOzd3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NXWtNFUh|ryP NIL0b3FUSU6JRWK=
human RKO cell M{LEfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmDlTY5pcWKrdHnvckBw\iCqdX3hckBTU09iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkS3[U0xPSEQvF2= NGLHe5ZUSU6JRWK=
human KYSE-510 cell MlvDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUnJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvNUGwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4{QGVvMEWg{txO M{HXZXNCVkeHUh?=
human HLE cell NV;URlBkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4DNRWlvcGmkaYTpc44hd2ZiaIXtZY4hUEyHIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD6yOoUuODVizszN M3;NcXNCVkeHUh?=
human MCF7 cell NFXpN2RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYTkOnNuUW6qaXLpeIlwdiCxZjDoeY1idiCPQ1[3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE43PmVvMEWg{txO Mo\WV2FPT0WU
human 786-0 cell NFr4fFJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3nBT2lvcGmkaYTpc44hd2ZiaIXtZY4hPzh4LUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlEyKG6P MlfkV2FPT0WU
human TE-8 cell Ml36S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXrtOGltUW6qaXLpeIlwdiCxZjDoeY1idiCWRT24JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOTFibl2= MoT4V2FPT0WU
ovarian carcinoma 1A9 cell NHW1UVNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3Ht[GlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IH;2ZZJq[W5iY3HyZ4lvd22jIEHBPUBk\WyuIHfyc5d1cA>? MkLDNVYyOzR7Mki=
human PANC-03-27 cell NWDZS3hbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkLpTY5pcWKrdHnvckBw\iCqdX3hckBRSU6FLUCzMVI4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zM{Kg{txO MlrUV2FPT0WU
human VMRC-RCZ cell NWrTToRTT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnGzTY5pcWKrdHnvckBw\iCqdX3hckBXVVKFLWLDXkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTN4IN88US=> M2HIbHNCVkeHUh?=
human IGROV-1 cell MlHaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlvQTY5pcWKrdHnvckBw\iCqdX3hckBKT1KRVj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPTZizszN MXvTRW5ITVJ?
human MG-63 cell MlLTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIS3UFBKdmirYnn0bY9vKG:oIHj1cYFvKE2JLU[zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPjVizszN NEG1O3lUSU6JRWK=
human SIG-M5 cell NG\oZlJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3zDeWlvcGmkaYTpc44hd2ZiaIXtZY4hW0mJLV21JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPjlizszN MkPQV2FPT0WU
human NCI-H650 cell NGHMSVVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmXnTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSE[1NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTdizszN NILvbWhUSU6JRWK=
human PSN1 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV3x[WNRUW6qaXLpeIlwdiCxZjDoeY1idiCSU16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPzNizszN NIfaNVJUSU6JRWK=
human PA-1 cell M1rJdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVHYW4xOUW6qaXLpeIlwdiCxZjDoeY1idiCSQT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPzRizszN MYrTRW5ITVJ?
KB-8511 cells Mo\2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkTnO|IhcA>? M3XX[2NwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDD0bIUh\3Kxd4ToJI9nKHCjY3zpeIF5\WxvcnXzbZN1[W62IHj1cYFvKGWyaXTldo1wcWRiY3HyZ4lvd22jIHPlcIx{KEuELUi1NVEh[nliNUCgdIVz[2WwdDCoO|IhcHJiZYjwc5N2emVrLDDJR|UxRTBwMUig{txO NWPLUFZjOTFzM{OwPFY>
KB-31 cells NWDMWXg{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoC4O|IhcA>? NF;iUplEd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgeIhmKGe{b4f0bEBw\iCyYXPsbZRigGWuLYPlcpNqfGm4ZTDoeY1idiCncHnk[ZJud2mmIHPhdoNqdm:vYTDj[YxteyCNQj2zNUBjgSB3MDDw[ZJk\W62IDi3NkBpeiCneIDvd5Vz\Sl? MlTkNVEyOzNyOE[=
human BHY cell Mk\LS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWrTbWU1UW6qaXLpeIlwdiCxZjDoeY1idiCESGmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE6PSCwTR?= NX3tPHlIW0GQR1XS
human BB30-HNC cell NIToV3ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmHJTY5pcWKrdHnvckBw\iCqdX3hckBDSjNyLVjOR{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjBzIN88US=> M3nvSXNCVkeHUh?=
human HOS cell M{DB[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWjJcohq[mm2aX;uJI9nKGi3bXHuJGhQWyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMkG1JO69VQ>? NVS5dG1IW0GQR1XS
human 639-V cell NIrHfWhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUPRUYdmUW6qaXLpeIlwdiCxZjDoeY1idiB4M{mtWkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjF6IN88US=> NF7rUIJUSU6JRWK=
human ES1 cell  NV7vZ|BVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGVUOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMkKxJO69VQ>? MoPUV2FPT0WU
human TE-15 cell  NHLSPW1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1rEUWlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIzPSEQvF2= M4TTfXNCVkeHUh?=
human LXF-289 cell NFnSTmtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWHJcohq[mm2aX;uJI9nKGi3bXHuJGxZTi1{OEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlI4PyEQvF2= M{fDTXNCVkeHUh?=
human CAL-12T cell M3zwO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mnr6TY5pcWKrdHnvckBw\iCqdX3hckBESUxvMULUJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOzlizszN NGPkfnVUSU6JRWK=
human T84 cell NWPuNIJFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1fMTWlvcGmkaYTpc44hd2ZiaIXtZY4hXDh2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yOEDPxE1? M{nHcHNCVkeHUh?=
human KYSE-450 cell MlS0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NILpcXdKdmirYnn0bY9vKG:oIHj1cYFvKEu\U1WtOFUxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5{Nkeg{txO M4G4SnNCVkeHUh?=
human LCLC-103H cell M4LvfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVvhU21SUW6qaXLpeIlwdiCxZjDoeY1idiCOQ1zDMVExO0hiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkOxJI5O MVvTRW5ITVJ?
human NCI-H810 cell NVjXO4xDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn3HTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEixNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzJ{IN88US=> MmjhV2FPT0WU
human HUTU-80 cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnXmTY5pcWKrdHnvckBw\iCqdX3hckBJXVSXLUiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{OjhizszN NFf2NHdUSU6JRWK=
human NCI-H460 cell MoDOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHvGcVdKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOFYxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5|M{Og{txO NYPyO4k{W0GQR1XS
human HGC-27 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYHJcohq[mm2aX;uJI9nKGi3bXHuJGhISy1{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuN|M5KM7:TR?= MXXTRW5ITVJ?
human HSC-2 cell M2PzXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF3kO5RKdmirYnn0bY9vKG:oIHj1cYFvKEiVQz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{PjNibl2= MnvDV2FPT0WU
human IA-LM cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3m0SWlvcGmkaYTpc44hd2ZiaIXtZY4hUUFvTF2gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM6OyEQvF2= MmXUV2FPT0WU
human HMV-II cell MlPpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoOxTY5pcWKrdHnvckBw\iCqdX3hckBJVVZvSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM6PCEQvF2= M1jHcnNCVkeHUh?=
human COLO-679 cell MmfhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M13VS2lvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz22O|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN7NDFOwG0> MY\TRW5ITVJ?
human NCI-H2122 cell NIXBbWNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlrhTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKxNlIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjRzIN88US=> NVj3emdPW0GQR1XS
human CAL-39 cell NIfqR5BIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWLJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOFEyKM7:TR?= NF;wT5FUSU6JRWK=
human SW954 cell MkTmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2fy[WlvcGmkaYTpc44hd2ZiaIXtZY4hW1d7NUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQ{PiEQvF2= M4ewUnNCVkeHUh?=
human LCLC-97TM1 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX;Y[nhQUW6qaXLpeIlwdiCxZjDoeY1idiCOQ1zDMVk4XE1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD60OFQh|ryP M{n3OnNCVkeHUh?=
human KYSE-270 cell M1vWSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIf3NYpKdmirYnn0bY9vKG:oIHj1cYFvKEu\U1WtNlcxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC52NEWg{txO M2HrNnNCVkeHUh?=
human NB13 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NED2dIxKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQ1PyEQvF2= Mn7vV2FPT0WU
human SK-LMS-1 cell NUHqT2NXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4XkR2lvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTF3TMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjZ2NjFOwG0> MYDTRW5ITVJ?
human SW620 cell MnXNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX3rV3hXUW6qaXLpeIlwdiCxZjDoeY1idiCVV{[yNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPjR6IN88US=> MoPZV2FPT0WU
human HCT-15 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmPXTY5pcWKrdHnvckBw\iCqdX3hckBJS1RvMUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlcyPyEQvF2= Mme1V2FPT0WU
human DU-145 cell NU\NeFZwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGjjZnVKdmirYnn0bY9vKG:oIHj1cYFvKESXLUG0OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPzJzIN88US=> Mo\kV2FPT0WU
human ST486 cell NHXZRnhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHPHSYVKdmirYnn0bY9vKG:oIHj1cYFvKFOWNEi2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE44OjFizszN NGnGNYhUSU6JRWK=
human A427 cell NWG2O3RXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4faXmlvcGmkaYTpc44hd2ZiaIXtZY4hSTR{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPFI5KM7:TR?= M1q1fnNCVkeHUh?=
human HT-1080 cell M4jmfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnzVTY5pcWKrdHnvckBw\iCqdX3hckBJXC1zMEiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PDJizszN NUXyUIhDW0GQR1XS
human NCI-H2009 cell NE\jNWVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NELBbFlKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlAxQSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwOE[yJO69VQ>? NXX5cHhiW0GQR1XS
human SK-LU-1 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnzVTY5pcWKrdHnvckBw\iCqdX3hckBUUy2OVT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PjZizszN NEjUNpBUSU6JRWK=
human SCC-4 cell NGDqOpdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUjGfYdHUW6qaXLpeIlwdiCxZjDoeY1idiCVQ1OtOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDd5IN88US=> NILaeWNUSU6JRWK=
human NCI-H1299 cell M1zQPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2jQNWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixNlk6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56ODFOwG0> NEPsWnFUSU6JRWK=
human NH-12 cell MnXoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVq0WnVKUW6qaXLpeIlwdiCxZjDoeY1idiCQSD2xNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDlzIN88US=> NUGzNWwxW0GQR1XS
human SK-UT-1 cell MlWzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4XuSGlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvVWStNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDl{IN88US=> MVnTRW5ITVJ?
human Bel7402 NGDPVZhRem:uaX\ldoF1cW:wIHHzd4F6 Ml3nO|IhcA>? NWexRVdjSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB{IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJiR3zvJIF{e2G7LDDJR|UxRTBwOTFOwG0> MXGyNlMzODN3NB?=
human A388 cell NUH4c|FyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkO5TY5pcWKrdHnvckBw\iCqdX3hckBCOzh6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD65OEDPxE1? NFnXdY5USU6JRWK=
human SW982 cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH64VVRKdmirYnn0bY9vKG:oIHj1cYFvKFOZOUiyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46QTlizszN NWnXXJV1W0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo In a mouse xenograft model of RPMI 8226 cells, Epothilone B (2.5 mg/kg–4 mg/kg) prolongs survival and suppresses tumor growth. [4] Similarly, in mouse xenograft models of prostate cancer cells, including DU145 and PC3, Epothilone B at the same dose also inhibits tumor growth. [6]

Protocol

Kinase Assay:[1]
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Tubulin polymerization assay:

Calf brain microtubule proteins (MTP) are purified, which includes approximately 15%–20% microtubule associated proteins. The buffer (MES buffer) used for the Epothilone B-microtubule studies contains 0.1 M 2-morpholinoethanesulfonic acid (MES), 1 mM EGTA, 0.5 mM MgCl2, and 3 M glycerol at pH 6.6. Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone B is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope and expressed as the mean with standard deviation obtained from three different concentrations.
Cell Research:[2]
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  • Cell lines: KB3-1, KBV-1, Hela, and Hs578T cells
  • Concentrations: 0–1 μM
  • Incubation Time: 72 hours
  • Method: For mitotic block and aberrant mitosis, cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips. After 24 hours, cells are treated with Epothilone B and scored at regular intervals. For the cytotoxicity analysis, cells are counted and scored as trypan blue positive or negative. Concurrently, coverslips andaliquots of cells in the culture supernatant are fixed and stained with Hoechst33342 in PBS. These cells are scored for cells blocked at the G2/M transition and aberrant mitosis.
    (Only for Reference)
Animal Research:[4]
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  • Animal Models: Mice xenograft model of RPMI 8226 cells
  • Dosages: 2.5 mg/kg–4 mg/kg
  • Administration: Inject intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 102 mg/mL (200.91 mM)
Ethanol 102 mg/mL (200.91 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.68
Formula

C27H41NO6S

CAS No. 152044-54-7
Storage powder
in solvent
Synonyms EPO906
Smiles CC1CCCC2(C(O2)CC(OC(=O)CC(C(C(=O)C(C1O)C)(C)C)O)C(=CC3=CSC(=N3)C)C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00496600 Completed Drug: Patupilone|Drug: RAD001 Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers The State University of New Jersey July 2007 Phase 1
NCT00442741 Withdrawn Drug: Patupilone|Drug: Patupilone + Omeprazole Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1
NCT00468260 Terminated Drug: Patupilone Advanced Malignancies Novartis Pharmaceuticals|Novartis May 2007 Phase 1
NCT00448396 Completed Drug: Patupilone Advanced Malignancies Novartis Pharmaceuticals|Novartis March 2007 Phase 1
NCT00426140 Completed Drug: Patupilone Advanced Malignancies|Solid Tumors Novartis Pharmaceuticals|Novartis August 2006 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Microtubule Associated Signaling Pathway Map

Related Microtubule Associated Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID