Patupilone (EPO906, Epothilone B)

Catalog No.S1364

Patupilone (EPO906, Epothilone B) Chemical Structure

Molecular Weight(MW): 507.68

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.

Size Price Stock Quantity  
In DMSO USD 250 In stock
USD 70 In stock
USD 120 In stock
USD 210 In stock
USD 470 In stock
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Cited by 7 Publications

4 Customer Reviews

  • Matrigel invasion assay of H1299 cells treated with SDF-1α or SDF-1α plus Epothilone B prior to 0 or 2 Gy irradiation. After the treatment for 12 h, the H1299 cells that migrated to the bottom surface of the membrane were stained with Giemsa, and the number of migrated cells was calculated manually.

    Oncotarget, 2015, 6(13):10893-907.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

  • Cells were seeded in 96 well paltes, and then treated with the indicated concentration of Epothilone B for 48 h. Cell survival was measured by a standarad MTT assay.

     

     

    Dr. Helen Sadik of Johns Hopkins University. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9. Patupilone (EPO906, Epothilone B) purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.
Targets
Tubulin [1]
(Cell-free assay)
1.8 μM(EC0.01)
In vitro

Epothilone B shows better activity than Epothilone A. The EC0.01 of Epothilone B is 1.8 μM. Epothilone B potently inhibits cell proliferation in HCT116 cells, with IC50 of 0.8 nM. [1] Epothilone B induces mitotic arrest and displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 of 3 nM to 92 nM. Epothilone B competes with Taxol in binding to microtubules, with IC50 of 3.3 μM. [2] In MCF-7 cells overexpressing GFP-α-tubulin, Epothilone B (3.5 nM) efficiently blocks microtubule dynamics. Meanwhile, Epothilone B induces mitotic arrest with IC50 of 3.5 nM. [3] In multiple myeloma (MM) cells, including RPMI 8226, U266, MM.1S, LR5, and MR20, Epothilone B directly suppresses proliferation with IC50 of 1 nM to 10 nM. Similarly, Epothilone B (10 nM) also induces cell cycle arrest and apoptosis. [4] A recent study reveals that, in ovarian cancer Hey cells, Epothilone B (5 nM–100 nM) enhances surface epithelial cell adhesion antigen (EpCAM), without affecting the transcription or the total cellular level of EpCAM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LC-2-ad cell MmLsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3\Vd2lvcGmkaYTpc44hd2ZiaIXtZY4hVENvMj3h[EBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPzenLUC2JO69VQ>? M2fLSXNCVkeHUh?=
human A431 cell MkG5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NE\yVmFKdmirYnn0bY9vKG:oIHj1cYFvKEF2M{GgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25Mlg3\S1yNjFOwG0> NGnEZmZUSU6JRWK=
human SW1710 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIeyPW9KdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOTSnLUC1JO69VQ>? NH3ReIhUSU6JRWK=
human Daoy cell MkG4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkG4TY5pcWKrdHnvckBw\iCqdX3hckBF[W:7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYpvwKwhUUN3ME2yMlc{\S1yNTFOwG0> M{niS3NCVkeHUh?=
human BFTC-905 cell M4Hjdmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWPEfm5PUW6qaXLpeIlwdiCxZjDoeY1idiCERmTDMVkxPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwOUnlMVA2KM7:TR?= MlvNV2FPT0WU
human A375 cell NH3BfnJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4TDS2lvcGmkaYTpc44hd2ZiaIXtZY4hSTN5NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUXlMVA2KM7:TR?= MWjTRW5ITVJ?
human RKO cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M334emlvcGmkaYTpc44hd2ZiaIXtZY4hWkuRIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj60O4UuODVizszN M1L5S3NCVkeHUh?=
human KYSE-510 cell M3j5O2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFXOfVRKdmirYnn0bY9vKG:oIHj1cYFvKEu\U1WtOVExKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5|OHWtNFUh|ryP NXe4W|h3W0GQR1XS
human HLE cell MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGS0TnFKdmirYnn0bY9vKG:oIHj1cYFvKEiORTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlZmNTB3IN88US=> NXu1dYh[W0GQR1XS
human MCF7 cell Mln4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVnJcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16Lk[2[U0xPSEQvF2= NYHiW|k4W0GQR1XS
human 786-0 cell Mn3iS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYH6XlV1UW6qaXLpeIlwdiCxZjDoeY1idiB5OE[tNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTFibl2= NF75fFZUSU6JRWK=
human TE-8 cell NHHrZYxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXLTXo1WUW6qaXLpeIlwdiCxZjDoeY1idiCWRT24JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOTFibl2= MlnsV2FPT0WU
ovarian carcinoma 1A9 cell NH61[|FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1\lXmlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IH;2ZZJq[W5iY3HyZ4lvd22jIEHBPUBk\WyuIHfyc5d1cA>? M3fL[FE3OTN2OUK4
human PANC-03-27 cell Ml;jS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mmq0TY5pcWKrdHnvckBw\iCqdX3hckBRSU6FLUCzMVI4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zM{Kg{txO MUDTRW5ITVJ?
human VMRC-RCZ cell NX[0flY2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKGi3bXHuJHZOWkNvUlPaJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOzZizszN MoHXV2FPT0WU
human IGROV-1 cell MmjOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4T0dmlvcGmkaYTpc44hd2ZiaIXtZY4hUUeUT2[tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTV4IN88US=> MUTTRW5ITVJ?
human MG-63 cell NULOdJZCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4HTXWlvcGmkaYTpc44hd2ZiaIXtZY4hVUdvNkOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE3PSEQvF2= NX;QSFh7W0GQR1XS
human SIG-M5 cell NUnqbZU1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{G0bGlvcGmkaYTpc44hd2ZiaIXtZY4hW0mJLV21JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPjlizszN NH3hVWVUSU6JRWK=
human NCI-H650 cell NVXHWlZNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFXzWXBKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOlUxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zNzFOwG0> MWrTRW5ITVJ?
human PSN1 cell MlvqS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHjndnJKdmirYnn0bY9vKG:oIHj1cYFvKFCVTkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE4OyEQvF2= M1fDR3NCVkeHUh?=
human PA-1 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml;YTY5pcWKrdHnvckBw\iCqdX3hckBRSS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xO|Qh|ryP MXzTRW5ITVJ?
KB-8511 cells MlP6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXO3NkBp MlywR49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IITo[UBoem:5dHigc4YheGGlbHn0ZZhmdC2{ZYPpd5RidnRiaIXtZY4h\XCrZHXycY9q\CClYYLjbY5wdWFiY3XscJMhU0JvOEWxNUBjgSB3MDDw[ZJk\W62IDi3NkBpeiCneIDvd5Vz\SluIFnDOVA:OC5zODFOwG0> NHLtU40yOTF|M{C4Oi=>
KB-31 cells NHPx[2dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml;uO|IhcA>? M2fjOGNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDD0bIUh\3Kxd4ToJI9nKHCjY3zpeIF5\Wxvc3Xud4l1cX[nIHj1cYFvKGWyaXTldo1wcWRiY3HyZ4lvd22jIHPlcIx{KEuELUOxJIJ6KDVyIIDldoNmdnRiKEeyJIhzKGW6cH;zeZJmMQ>? NYq4O2RmOTFzM{OwPFY>
human BHY cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnyyTY5pcWKrdHnvckBw\iCqdX3hckBDUFliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkG5OUBvVQ>? NInMb|BUSU6JRWK=
human BB30-HNC cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGJDOzBvSF7DJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zODFizszN MlLoV2FPT0WU
human HOS cell MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXjjVG53UW6qaXLpeIlwdiCxZjDoeY1idiCKT2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIyPSEQvF2= MUHTRW5ITVJ?
human 639-V cell NFvqcJZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3LQRmlvcGmkaYTpc44hd2ZiaIXtZY4hPjN7LW[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIyQCEQvF2= NGnu[pJUSU6JRWK=
human ES1 cell  NVq5d|RyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlzITY5pcWKrdHnvckBw\iCqdX3hckBGWzFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkKyNUDPxE1? NHHRZnFUSU6JRWK=
human TE-15 cell  MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHeyUVRKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUG1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOjVizszN NITk[G5USU6JRWK=
human LXF-289 cell MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NG\IOZlKdmirYnn0bY9vKG:oIHj1cYFvKEy[Rj2yPFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJ5NzFOwG0> NVW5XphZW0GQR1XS
human CAL-12T cell M{OzdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVjJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1zMmSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlI{QSEQvF2= MXHTRW5ITVJ?
human T84 cell NImyWpFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M17FPGlvcGmkaYTpc44hd2ZiaIXtZY4hXDh2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yOEDPxE1? Mki2V2FPT0WU
human KYSE-450 cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{TKSGlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT20OVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJ4NzFOwG0> NUDJPWJbW0GQR1XS
human LCLC-103H cell NUDHOFhKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIDuOVlKdmirYnn0bY9vKG:oIHj1cYFvKEyFTFOtNVA{UCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwM{Ggcm0> NYTIVFM5W0GQR1XS
human NCI-H810 cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3;acWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi4NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN{MjFOwG0> M1XkVXNCVkeHUh?=
human HUTU-80 cell NHvwWHRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIXMSnpKdmirYnn0bY9vKG:oIHj1cYFvKEiXVGWtPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN{ODFOwG0> NX24bI9xW0GQR1XS
human NCI-H460 cell NUDQR2UzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFLRW|VKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOFYxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5|M{Og{txO NGLkRYZUSU6JRWK=
human HGC-27 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIjp[phKdmirYnn0bY9vKG:oIHj1cYFvKEiJQz2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzN6IN88US=> M3PXRXNCVkeHUh?=
human HSC-2 cell NYDSeVFxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1LQOWlvcGmkaYTpc44hd2ZiaIXtZY4hUFOFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM3OyCwTR?= NX24eWQ5W0GQR1XS
human IA-LM cell MnO0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml3WTY5pcWKrdHnvckBw\iCqdX3hckBKSS2OTTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuN|k{KM7:TR?= NUDmRpFoW0GQR1XS
human HMV-II cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoLMTY5pcWKrdHnvckBw\iCqdX3hckBJVVZvSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM6PCEQvF2= MUnTRW5ITVJ?
human COLO-679 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3XRWmlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz22O|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN7NDFOwG0> MXPTRW5ITVJ?
human NCI-H2122 cell NXHxVZNlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mm\xTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKxNlIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjRzIN88US=> MUXTRW5ITVJ?
human CAL-39 cell M2qwVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3vZR2lvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE41OTFizszN M1T5PHNCVkeHUh?=
human SW954 cell MmrLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlOwTY5pcWKrdHnvckBw\iCqdX3hckBUXzl3NDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOFM3KM7:TR?= MVTTRW5ITVJ?
human LCLC-97TM1 cell Mnv5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV;Jcohq[mm2aX;uJI9nKGi3bXHuJGxEVENvOUfUUVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjR2NDFOwG0> M3jlZnNCVkeHUh?=
human KYSE-270 cell MnPMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYfZVYQ3UW6qaXLpeIlwdiCxZjDoeY1idiCNWWPFMVI4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNES1JO69VQ>? M{XxT3NCVkeHUh?=
human NB13 cell NFrRW2FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWPJcohq[mm2aX;uJI9nKGi3bXHuJG5DOTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkS0O{DPxE1? MmTXV2FPT0WU
human SK-LMS-1 cell Mk\PS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4flPWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTF3TMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjZ2NjFOwG0> M1u1OXNCVkeHUh?=
human SW620 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFnFOopKdmirYnn0bY9vKG:oIHj1cYFvKFOZNkKwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE43PDhizszN Mlj0V2FPT0WU
human HCT-15 cell NUTJfow{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mm\3TY5pcWKrdHnvckBw\iCqdX3hckBJS1RvMUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlcyPyEQvF2= NVe3em96W0GQR1XS
human DU-145 cell NWruU3JTT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUHJcohq[mm2aX;uJI9nKGi3bXHuJGRWNTF2NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|IyKM7:TR?= MUXTRW5ITVJ?
human ST486 cell NI[xSZpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmHwTY5pcWKrdHnvckBw\iCqdX3hckBUXDR6NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|IyKM7:TR?= NHr4S21USU6JRWK=
human A427 cell NWHhbo5ZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX7nUHV4UW6qaXLpeIlwdiCxZjDoeY1idiCDNEK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45OjhizszN MWnTRW5ITVJ?
human HT-1080 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mo\WTY5pcWKrdHnvckBw\iCqdX3hckBJXC1zMEiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PDJizszN MmC0V2FPT0WU
human NCI-H2009 cell Mm\nS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIXPeWpKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlAxQSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwOE[yJO69VQ>? NHKweYhUSU6JRWK=
human SK-LU-1 cell MoXDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFf6UlFKdmirYnn0bY9vKG:oIHj1cYFvKFONLVzVMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh4NjFOwG0> M4[0c3NCVkeHUh?=
human SCC-4 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFrCcVVKdmirYnn0bY9vKG:oIHj1cYFvKFOFQz20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PzdizszN NF3yPY9USU6JRWK=
human NCI-H1299 cell NGq1OFVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV3Jcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUK5PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDhizszN M2LPRnNCVkeHUh?=
human NH-12 cell M2fQVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn;5TY5pcWKrdHnvckBw\iCqdX3hckBPUC1zMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPFkyKM7:TR?= M1jk[XNCVkeHUh?=
human SK-UT-1 cell MnPtS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4DNb2lvcGmkaYTpc44hd2ZiaIXtZY4hW0tvVWStNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDl{IN88US=> MVnTRW5ITVJ?
human Bel7402 MlHGVJJwdGmoZYLheIlwdiCjc4PhfS=> MV63NkBp NXvDWFdtSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB{IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJiR3zvJIF{e2G7LDDJR|UxRTBwOTFOwG0> M1TDNFIzOzJyM{W0
human A388 cell MkHkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYjac2VWUW6qaXLpeIlwdiCxZjDoeY1idiCDM{i4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46PCEQvF2= MWnTRW5ITVJ?
human SW982 cell MlLVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFfifnhKdmirYnn0bY9vKG:oIHj1cYFvKFOZOUiyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46QTlizszN MVTTRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo In a mouse xenograft model of RPMI 8226 cells, Epothilone B (2.5 mg/kg–4 mg/kg) prolongs survival and suppresses tumor growth. [4] Similarly, in mouse xenograft models of prostate cancer cells, including DU145 and PC3, Epothilone B at the same dose also inhibits tumor growth. [6]

Protocol

Kinase Assay:[1]
+ Expand

Tubulin polymerization assay:

Calf brain microtubule proteins (MTP) are purified, which includes approximately 15%–20% microtubule associated proteins. The buffer (MES buffer) used for the Epothilone B-microtubule studies contains 0.1 M 2-morpholinoethanesulfonic acid (MES), 1 mM EGTA, 0.5 mM MgCl2, and 3 M glycerol at pH 6.6. Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone B is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope and expressed as the mean with standard deviation obtained from three different concentrations.
Cell Research:[2]
+ Expand
  • Cell lines: KB3-1, KBV-1, Hela, and Hs578T cells
  • Concentrations: 0–1 μM
  • Incubation Time: 72 hours
  • Method: For mitotic block and aberrant mitosis, cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips. After 24 hours, cells are treated with Epothilone B and scored at regular intervals. For the cytotoxicity analysis, cells are counted and scored as trypan blue positive or negative. Concurrently, coverslips andaliquots of cells in the culture supernatant are fixed and stained with Hoechst33342 in PBS. These cells are scored for cells blocked at the G2/M transition and aberrant mitosis.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Mice xenograft model of RPMI 8226 cells
  • Formulation: Dissolved in30% PEG-300
  • Dosages: 2.5 mg/kg–4 mg/kg
  • Administration: Inject intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 102 mg/mL (200.91 mM)
Ethanol 102 mg/mL (200.91 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.68
Formula

C27H41NO6S

CAS No. 152044-54-7
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00496600 Completed Drug: Patupilone|Drug: RAD001 Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers The State University of New Jersey July 2007 Phase 1
NCT00442741 Withdrawn Drug: Patupilone|Drug: Patupilone + Omeprazole Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1
NCT00468260 Terminated Drug: Patupilone Advanced Malignancies Novartis Pharmaceuticals|Novartis May 2007 Phase 1
NCT00448396 Completed Drug: Patupilone Advanced Malignancies Novartis Pharmaceuticals|Novartis March 2007 Phase 1
NCT00426140 Completed Drug: Patupilone Advanced Malignancies|Solid Tumors Novartis Pharmaceuticals|Novartis August 2006 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Microtubule Associated Signaling Pathway Map

Related Microtubule Associated Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID