Patupilone (EPO906, Epothilone B)

Catalog No.S1364

Patupilone (EPO906, Epothilone B) Chemical Structure

Molecular Weight(MW): 507.68

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.

Size Price Stock Quantity  
In DMSO USD 250 In stock
USD 70 In stock
USD 120 In stock
USD 210 In stock
USD 470 In stock
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Cited by 5 Publications

4 Customer Reviews

  • Matrigel invasion assay of H1299 cells treated with SDF-1α or SDF-1α plus Epothilone B prior to 0 or 2 Gy irradiation. After the treatment for 12 h, the H1299 cells that migrated to the bottom surface of the membrane were stained with Giemsa, and the number of migrated cells was calculated manually.

    Oncotarget, 2015, 6(13):10893-907.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9. Patupilone (EPO906, Epothilone B) purchased from Selleck.

  • Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Cells were seeded in 96 well paltes, and then treated with the indicated concentration of Epothilone B for 48 h. Cell survival was measured by a standarad MTT assay.

     

     

    Dr. Helen Sadik of Johns Hopkins University. Patupilone (EPO906, Epothilone B) purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.
Targets
Tubulin [1]
(Cell-free assay)
1.8 μM(EC0.01)
In vitro

Epothilone B shows better activity than Epothilone A. The EC0.01 of Epothilone B is 1.8 μM. Epothilone B potently inhibits cell proliferation in HCT116 cells, with IC50 of 0.8 nM. [1] Epothilone B induces mitotic arrest and displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 of 3 nM to 92 nM. Epothilone B competes with Taxol in binding to microtubules, with IC50 of 3.3 μM. [2] In MCF-7 cells overexpressing GFP-α-tubulin, Epothilone B (3.5 nM) efficiently blocks microtubule dynamics. Meanwhile, Epothilone B induces mitotic arrest with IC50 of 3.5 nM. [3] In multiple myeloma (MM) cells, including RPMI 8226, U266, MM.1S, LR5, and MR20, Epothilone B directly suppresses proliferation with IC50 of 1 nM to 10 nM. Similarly, Epothilone B (10 nM) also induces cell cycle arrest and apoptosis. [4] A recent study reveals that, in ovarian cancer Hey cells, Epothilone B (5 nM–100 nM) enhances surface epithelial cell adhesion antigen (EpCAM), without affecting the transcription or the total cellular level of EpCAM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LC-2-ad cell MkS4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX;QPGNjUW6qaXLpeIlwdiCxZjDoeY1idiCOQz2yMYFlKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy55N3WtNFYh|ryP NYq1NnBzW0GQR1XS
human A431 cell MnrNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXnzXGl3UW6qaXLpeIlwdiCxZjDoeY1idiCDNEOxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU45PmVvME[g{txO NIr5[5pUSU6JRWK=
human SW1710 cell MmDyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV;McYRLUW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjF2ZT2wOUDPxE1? M37zZnNCVkeHUh?=
human Daoy cell NXTNNpVST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkXxTY5pcWKrdHnvckBw\iCqdX3hckBF[W:7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYpvwKwhUUN3ME2yMlc{\S1yNTFOwG0> NH[wc3VUSU6JRWK=
human BFTC-905 cell NYLNSmNIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYHJcohq[mm2aX;uJI9nKGi3bXHuJGJHXENvOUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE46QWVvMEWg{txO MYjTRW5ITVJ?
human A375 cell MmTJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEnQSYVKdmirYnn0bY9vKG:oIHj1cYFvKEF|N{WgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21[U0xPSEQvF2= M1HN[XNCVkeHUh?=
human RKO cell NVXHelQ6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1ewSmlvcGmkaYTpc44hd2ZiaIXtZY4hWkuRIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj60O4UuODVizszN NVW0Nml2W0GQR1XS
human KYSE-510 cell MnXoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFGyU3VKdmirYnn0bY9vKG:oIHj1cYFvKEu\U1WtOVExKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5|OHWtNFUh|ryP M1fZU3NCVkeHUh?=
human HLE cell M1i0S2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVH4RW9SUW6qaXLpeIlwdiCxZjDoeY1idiCKTFWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlI3\S1yNTFOwG0> M1nFVnNCVkeHUh?=
human MCF7 cell NVvhbmZ{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWPJcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16Lk[2[U0xPSEQvF2= NIHDT2ZUSU6JRWK=
human 786-0 cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF23U3BKdmirYnn0bY9vKG:oIHj1cYFvKDd6Nj2wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOSCwTR?= M1jBenNCVkeHUh?=
human TE-8 cell MnLJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1PrSGlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVEyKG6P NHTldIlUSU6JRWK=
ovarian carcinoma 1A9 cell MoDoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX32emlbUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4Sgc5ZiemmjbjDjZZJkcW6xbXGgNWE6KGOnbHyg[5Jwf3Sq M4S5b|E3OTN2OUK4
human PANC-03-27 cell MoDsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M33YVGlvcGmkaYTpc44hd2ZiaIXtZY4hWEGQQz2wN{0zPyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMUOyJO69VQ>? M3zVPHNCVkeHUh?=
human VMRC-RCZ cell NXHaPVFbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1rxV2lvcGmkaYTpc44hd2ZiaIXtZY4hXk2UQz3SR3oh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF|NjFOwG0> M1LGcnNCVkeHUh?=
human IGROV-1 cell NWLtTpBGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUjXPYQ6UW6qaXLpeIlwdiCxZjDoeY1idiCLR2LPWk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zNU[g{txO MmDhV2FPT0WU
human MG-63 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIjNXlZKdmirYnn0bY9vKG:oIHj1cYFvKE2JLU[zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPjVizszN M13nSnNCVkeHUh?=
human SIG-M5 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3;tW2lvcGmkaYTpc44hd2ZiaIXtZY4hW0mJLV21JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPjlizszN MkXUV2FPT0WU
human NCI-H650 cell NIWzSZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVTJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNkWwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPyEQvF2= MV7TRW5ITVJ?
human PSN1 cell NWrNSlVIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkXkTY5pcWKrdHnvckBw\iCqdX3hckBRW05zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xO|Mh|ryP NHnj[nZUSU6JRWK=
human PA-1 cell NV23cHhyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2q4e2lvcGmkaYTpc44hd2ZiaIXtZY4hWEFvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVc1KM7:TR?= MXTTRW5ITVJ?
KB-8511 cells M{TESWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Moj4O|IhcA>? Ml2xR49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IITo[UBoem:5dHigc4YheGGlbHn0ZZhmdC2{ZYPpd5RidnRiaIXtZY4h\XCrZHXycY9q\CClYYLjbY5wdWFiY3XscJMhU0JvOEWxNUBjgSB3MDDw[ZJk\W62IDi3NkBpeiCneIDvd5Vz\SluIFnDOVA:OC5zODFOwG0> NU\Yd2x6OTFzM{OwPFY>
KB-31 cells NEfPW4RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmnFO|IhcA>? MV\Dc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIHnubIljcXRidHjlJIdzd3e2aDDv[kBx[WOuaYThfIVtNXOnboPpeIl3\SCqdX3hckBmeGmmZYLtc4llKGOjcnPpco9u[SClZXzsd{BMSi1|MTDifUA2OCCyZYLj[Y51KCh5MjDodkBmgHCxc4Xy[Uk> MXexNVE{OzB6Nh?=
human BHY cell M2T1N2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXPTcHRuUW6qaXLpeIlwdiCxZjDoeY1idiCESGmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE6PSCwTR?= NXmzdpVLW0GQR1XS
human BB30-HNC cell M3e3VGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF21[HFKdmirYnn0bY9vKG:oIHj1cYFvKEKEM{CtTG5EKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5{MEGg{txO MUjTRW5ITVJ?
human HOS cell M4rDRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M171eWlvcGmkaYTpc44hd2ZiaIXtZY4hUE:VIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNVUh|ryP MXHTRW5ITVJ?
human 639-V cell M3HwdWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFrmW5VKdmirYnn0bY9vKG:oIHj1cYFvKDZ|OT3WJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOThizszN MVLTRW5ITVJ?
human ES1 cell  NELPVXFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{G2emlvcGmkaYTpc44hd2ZiaIXtZY4hTVNzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlEh|ryP Ml;2V2FPT0WU
human TE-15 cell  MnLIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUjQR|NvUW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjJ3IN88US=> M2DTVXNCVkeHUh?=
human LXF-289 cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWTDe|hwUW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5{N{eg{txO Mo\ZV2FPT0WU
human CAL-12T cell MnLRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mmf0TY5pcWKrdHnvckBw\iCqdX3hckBESUxvMULUJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOzlizszN MkPwV2FPT0WU
human T84 cell NFHF[2hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUPVUphqUW6qaXLpeIlwdiCxZjDoeY1idiCWOESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlI1KM7:TR?= MnXiV2FPT0WU
human KYSE-450 cell MljXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{HqPGlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT20OVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJ4NzFOwG0> NIHD[5JUSU6JRWK=
human LCLC-103H cell NGj5NoZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGxEVENvMUCzTEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzFibl2= MljQV2FPT0WU
human NCI-H810 cell MkmxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M33sXmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi4NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN{MjFOwG0> M4nXOHNCVkeHUh?=
human HUTU-80 cell M{fVVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnW5TY5pcWKrdHnvckBw\iCqdX3hckBJXVSXLUiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{OjhizszN Moj3V2FPT0WU
human NCI-H460 cell MleyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NICydJNKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOFYxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5|M{Og{txO NVvRTlNLW0GQR1XS
human HGC-27 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWjuRVM3UW6qaXLpeIlwdiCxZjDoeY1idiCKR1OtNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN|ODFOwG0> MYfTRW5ITVJ?
human HSC-2 cell NWniU2R[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3G4SGlvcGmkaYTpc44hd2ZiaIXtZY4hUFOFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM3OyCwTR?= M4nvZ3NCVkeHUh?=
human IA-LM cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmfzTY5pcWKrdHnvckBw\iCqdX3hckBKSS2OTTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuN|k{KM7:TR?= NFfpOI9USU6JRWK=
human HMV-II cell M2Xhbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWXJcohq[mm2aX;uJI9nKGi3bXHuJGhOXi2LSTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuN|k1KM7:TR?= NUK3V21kW0GQR1XS
human COLO-679 cell M4r2V2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4LNeGlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz22O|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN7NDFOwG0> NYfsZYV7W0GQR1XS
human NCI-H2122 cell NF[zfZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYfHSVV7UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIyOjJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkSxJO69VQ>? NIG0OpNUSU6JRWK=
human CAL-39 cell MlPvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXrJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOFEyKM7:TR?= NWG3THU4W0GQR1XS
human SW954 cell MoG2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVf5UYZMUW6qaXLpeIlwdiCxZjDoeY1idiCVV{m1OEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPDN4IN88US=> NUnpfG51W0GQR1XS
human LCLC-97TM1 cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXn0cHptUW6qaXLpeIlwdiCxZjDoeY1idiCOQ1zDMVk4XE1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD60OFQh|ryP Mn7pV2FPT0WU
human KYSE-270 cell M2LjVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWnJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvMkewJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE41PDVizszN NGTofVZUSU6JRWK=
human NB13 cell MnzTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXPJcohq[mm2aX;uJI9nKGi3bXHuJG5DOTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkS0O{DPxE1? M3HQfnNCVkeHUh?=
human SK-LMS-1 cell NGnZdYZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{LOfWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTF3TMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjZ2NjFOwG0> NXu5dZZXW0GQR1XS
human SW620 cell NXnUdYJnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUH6d4R[UW6qaXLpeIlwdiCxZjDoeY1idiCVV{[yNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPjR6IN88US=> MXTTRW5ITVJ?
human HCT-15 cell NGDaO5NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJGhEXC1zNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|E4KM7:TR?= NXP1XGJSW0GQR1XS
human DU-145 cell M3nHbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUXJcohq[mm2aX;uJI9nKGi3bXHuJGRWNTF2NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|IyKM7:TR?= Moq3V2FPT0WU
human ST486 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4q1dWlvcGmkaYTpc44hd2ZiaIXtZY4hW1R2OE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlczOSEQvF2= MmrHV2FPT0WU
human A427 cell NV\aUWNVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnjRTY5pcWKrdHnvckBw\iCqdX3hckBCPDJ5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD64Nlgh|ryP MVjTRW5ITVJ?
human HT-1080 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH;DV3JKdmirYnn0bY9vKG:oIHj1cYFvKEiWLUGwPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh2MjFOwG0> NXHVfpFVW0GQR1XS
human NCI-H2009 cell Mm\2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUfJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkCwPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDZ{IN88US=> MXjTRW5ITVJ?
human SK-LU-1 cell NGq3T3lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXe2NW5FUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56Nk[g{txO M1ToO3NCVkeHUh?=
human SCC-4 cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4LCXWlvcGmkaYTpc44hd2ZiaIXtZY4hW0OFLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg4PyEQvF2= NUXsfHkzW0GQR1XS
human NCI-H1299 cell NGTvWFBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUfJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUK5PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDhizszN NXK1UWZEW0GQR1XS
human NH-12 cell M4jNVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mon5TY5pcWKrdHnvckBw\iCqdX3hckBPUC1zMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPFkyKM7:TR?= NYTlc|VnW0GQR1XS
human SK-UT-1 cell NYXiOldOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXXJcohq[mm2aX;uJI9nKGi3bXHuJHNMNVWWLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg6OiEQvF2= M4XK[3NCVkeHUh?=
human Bel7402 Ml\oVJJwdGmoZYLheIlwdiCjc4PhfS=> M2\yRVczKGh? M3fKUGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQnXsO|QxOiCjZoTldkA4OiCqcoOgZpkhS2WubGTpeIVzKEeubzDhd5NigSxiSVO1NF0xNjlizszN Mn7PNlI{OjB|NUS=
human A388 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYDIXop[UW6qaXLpeIlwdiCxZjDoeY1idiCDM{i4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46PCEQvF2= NH\SS5dUSU6JRWK=
human SW982 cell NXfhRpV1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{G1dGlvcGmkaYTpc44hd2ZiaIXtZY4hW1d7OEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlk6QSEQvF2= NH6ze5pUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo In a mouse xenograft model of RPMI 8226 cells, Epothilone B (2.5 mg/kg–4 mg/kg) prolongs survival and suppresses tumor growth. [4] Similarly, in mouse xenograft models of prostate cancer cells, including DU145 and PC3, Epothilone B at the same dose also inhibits tumor growth. [6]

Protocol

Kinase Assay:[1]
+ Expand

Tubulin polymerization assay:

Calf brain microtubule proteins (MTP) are purified, which includes approximately 15%–20% microtubule associated proteins. The buffer (MES buffer) used for the Epothilone B-microtubule studies contains 0.1 M 2-morpholinoethanesulfonic acid (MES), 1 mM EGTA, 0.5 mM MgCl2, and 3 M glycerol at pH 6.6. Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone B is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope and expressed as the mean with standard deviation obtained from three different concentrations.
Cell Research:[2]
+ Expand
  • Cell lines: KB3-1, KBV-1, Hela, and Hs578T cells
  • Concentrations: 0–1 μM
  • Incubation Time: 72 hours
  • Method: For mitotic block and aberrant mitosis, cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips. After 24 hours, cells are treated with Epothilone B and scored at regular intervals. For the cytotoxicity analysis, cells are counted and scored as trypan blue positive or negative. Concurrently, coverslips andaliquots of cells in the culture supernatant are fixed and stained with Hoechst33342 in PBS. These cells are scored for cells blocked at the G2/M transition and aberrant mitosis.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Mice xenograft model of RPMI 8226 cells
  • Formulation: Dissolved in30% PEG-300
  • Dosages: 2.5 mg/kg–4 mg/kg
  • Administration: Inject intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 102 mg/mL (200.91 mM)
Ethanol 102 mg/mL (200.91 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.68
Formula

C27H41NO6S

CAS No. 152044-54-7
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00715013 Completed Recurrent Glioblastoma Planned for Reoperation University of Zurich July 2008 Phase 1|Phase 2
NCT00715013 Completed Recurrent Glioblastoma Planned for Reoperation University of Zurich July 2008 Phase 1|Phase 2
NCT00496600 Completed Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers The State University of New Jersey July 2007 Phase 1
NCT00442741 Withdrawn Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1
NCT00496600 Completed Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers The State University of New Jersey July 2007 Phase 1
NCT00442741 Withdrawn Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Microtubule Associated Signaling Pathway Map

Related Microtubule Associated Products0

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID