Patupilone (EPO906, Epothilone B)

Catalog No.S1364

Patupilone (EPO906, Epothilone B) Chemical Structure

Molecular Weight(MW): 507.68

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.

Size Price Stock Quantity  
In DMSO USD 250 In stock
USD 70 In stock
USD 120 In stock
USD 210 In stock
USD 470 In stock
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Cited by 5 Publications

4 Customer Reviews

  • Matrigel invasion assay of H1299 cells treated with SDF-1α or SDF-1α plus Epothilone B prior to 0 or 2 Gy irradiation. After the treatment for 12 h, the H1299 cells that migrated to the bottom surface of the membrane were stained with Giemsa, and the number of migrated cells was calculated manually.

    Oncotarget, 2015, 6(13):10893-907.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9. Patupilone (EPO906, Epothilone B) purchased from Selleck.

  • Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Cells were seeded in 96 well paltes, and then treated with the indicated concentration of Epothilone B for 48 h. Cell survival was measured by a standarad MTT assay.

     

     

    Dr. Helen Sadik of Johns Hopkins University. Patupilone (EPO906, Epothilone B) purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.
Targets
Tubulin [1]
(Cell-free assay)
1.8 μM(EC0.01)
In vitro

Epothilone B shows better activity than Epothilone A. The EC0.01 of Epothilone B is 1.8 μM. Epothilone B potently inhibits cell proliferation in HCT116 cells, with IC50 of 0.8 nM. [1] Epothilone B induces mitotic arrest and displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 of 3 nM to 92 nM. Epothilone B competes with Taxol in binding to microtubules, with IC50 of 3.3 μM. [2] In MCF-7 cells overexpressing GFP-α-tubulin, Epothilone B (3.5 nM) efficiently blocks microtubule dynamics. Meanwhile, Epothilone B induces mitotic arrest with IC50 of 3.5 nM. [3] In multiple myeloma (MM) cells, including RPMI 8226, U266, MM.1S, LR5, and MR20, Epothilone B directly suppresses proliferation with IC50 of 1 nM to 10 nM. Similarly, Epothilone B (10 nM) also induces cell cycle arrest and apoptosis. [4] A recent study reveals that, in ovarian cancer Hey cells, Epothilone B (5 nM–100 nM) enhances surface epithelial cell adhesion antigen (EpCAM), without affecting the transcription or the total cellular level of EpCAM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LC-2-ad cell M3fNOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1HoR2lvcGmkaYTpc44hd2ZiaIXtZY4hVENvMj3h[EBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPzenLUC2JO69VQ>? MVLTRW5ITVJ?
human A431 cell M2D4emdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWrTV3VoUW6qaXLpeIlwdiCxZjDoeY1idiCDNEOxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU45PmVvME[g{txO MUjTRW5ITVJ?
human SW1710 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWHQcGF5UW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjF2ZT2wOUDPxE1? M1j2e3NCVkeHUh?=
human Daoy cell NFXsVVNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVrJcohq[mm2aX;uJI9nKGi3bXHuJGRid3liY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3Ojef-8kEBKSzVyPUKuO|NmNTB3IN88US=> MlfBV2FPT0WU
human BFTC-905 cell MoC1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2jGfGlvcGmkaYTpc44hd2ZiaIXtZY4hSk[WQz25NFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01Njl7ZT2wOUDPxE1? NVf2cXNkW0GQR1XS
human A375 cell NYnzXXRDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEDicVJKdmirYnn0bY9vKG:oIHj1cYFvKEF|N{WgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21[U0xPSEQvF2= NIfucGtUSU6JRWK=
human RKO cell NGjFdG9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYL3NGZZUW6qaXLpeIlwdiCxZjDoeY1idiCUS1:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlQ4\S1yNTFOwG0> MlftV2FPT0WU
human KYSE-510 cell NEH0b45Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVHJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvNUGwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4{QGVvMEWg{txO MoLtV2FPT0WU
human HLE cell NVnMWpB{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWLJcohq[mm2aX;uJI9nKGi3bXHuJGhNTSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRThwMk\lMVA2KM7:TR?= Mn;mV2FPT0WU
human MCF7 cell NF7TVHFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVjp[2RPUW6qaXLpeIlwdiCxZjDoeY1idiCPQ1[3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE43PmVvMEWg{txO MojWV2FPT0WU
human 786-0 cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFHrSotKdmirYnn0bY9vKG:oIHj1cYFvKDd6Nj2wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOSCwTR?= MXPTRW5ITVJ?
human TE-8 cell NH:zeVdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnPNTY5pcWKrdHnvckBw\iCqdX3hckBVTS16IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xNVEhdk1? NWjhSHl[W0GQR1XS
ovarian carcinoma 1A9 cell NELJPYhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF;wV4xKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDveoFzcWGwIHPhdoNqdm:vYTCxRVkh[2WubDDndo94fGh? MYCxOlE{PDl{OB?=
human PANC-03-27 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlHQTY5pcWKrdHnvckBw\iCqdX3hckBRSU6FLUCzMVI4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zM{Kg{txO MoqyV2FPT0WU
human VMRC-RCZ cell MmnMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NI\FVopKdmirYnn0bY9vKG:oIHj1cYFvKF[PUlOtVmNbKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zM{[g{txO MXfTRW5ITVJ?
human IGROV-1 cell M1LSbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUHJcohq[mm2aX;uJI9nKGi3bXHuJGlIWk:YLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE2PiEQvF2= NVf1OHg3W0GQR1XS
human MG-63 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnvCTY5pcWKrdHnvckBw\iCqdX3hckBOTy14MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVY2KM7:TR?= M2DaWnNCVkeHUh?=
human SIG-M5 cell MlTRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWK0XIVtUW6qaXLpeIlwdiCxZjDoeY1idiCVSVetUVUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF4OTFOwG0> M4jYOXNCVkeHUh?=
human NCI-H650 cell NHLGeo9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnXWTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSE[1NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTdizszN NHHqSHJUSU6JRWK=
human PSN1 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX;ueGVzUW6qaXLpeIlwdiCxZjDoeY1idiCSU16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPzNizszN M2rVbXNCVkeHUh?=
human PA-1 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYXIPI9FUW6qaXLpeIlwdiCxZjDoeY1idiCSQT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPzRizszN NWTLZ4RlW0GQR1XS
KB-8511 cells MknMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NU\DXphTPzJiaB?= NIrvZnZEd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgeIhmKGe{b4f0bEBw\iCyYXPsbZRigGWuLYLld4l{fGGwdDDoeY1idiCncHnk[ZJud2mmIHPhdoNqdm:vYTDj[YxteyCNQj24OVEyKGK7IEWwJJBmemOnboSgLFczKGi{IHX4dI9{fXKnKTygTWM2OD1yLkG4JO69VQ>? MoThNVEyOzNyOE[=
KB-31 cells M3i2bGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1j0PVczKGh? NVHzNJVtS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JJRp\SCpcn;3eIghd2ZicHHjcIl1[XinbD3z[Y5{cXSrdnWgbJVu[W5iZYDp[IVzdW:rZDDjZZJkcW6xbXGgZ4VtdHNiS1KtN|Eh[nliNUCgdIVz[2WwdDCoO|IhcHJiZYjwc5N2emVr M1Hs[|EyOTN|MEi2
human BHY cell MmDCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkjyTY5pcWKrdHnvckBw\iCqdX3hckBDUFliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkG5OUBvVQ>? MoHnV2FPT0WU
human BB30-HNC cell NFTWcoxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVLJcohq[mm2aX;uJI9nKGi3bXHuJGJDOzBvSF7DJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zODFizszN NHzHcZZUSU6JRWK=
human HOS cell MnrGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXrJcohq[mm2aX;uJI9nKGi3bXHuJGhQWyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMkG1JO69VQ>? MnjHV2FPT0WU
human 639-V cell M1HsNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYqydZpxUW6qaXLpeIlwdiCxZjDoeY1idiB4M{mtWkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjF6IN88US=> Mn7qV2FPT0WU
human ES1 cell  M171VGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmLKTY5pcWKrdHnvckBw\iCqdX3hckBGWzFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkKyNUDPxE1? MoXLV2FPT0WU
human TE-15 cell  NUDse4oyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{jkcWlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIzPSEQvF2= MWjTRW5ITVJ?
human LXF-289 cell M3KwO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHvuUGRKdmirYnn0bY9vKG:oIHj1cYFvKEy[Rj2yPFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJ5NzFOwG0> NX3R[ZZ1W0GQR1XS
human CAL-12T cell MkfzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnfWTY5pcWKrdHnvckBw\iCqdX3hckBESUxvMULUJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOzlizszN Mn\4V2FPT0WU
human T84 cell NV\rU5N6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHTXb4xKdmirYnn0bY9vKG:oIHj1cYFvKFR6NDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNlQh|ryP Mn2xV2FPT0WU
human KYSE-450 cell NHv0S2lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnixTY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUS1NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjZ5IN88US=> M4mx[XNCVkeHUh?=
human LCLC-103H cell NHj3WnVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGnCTXJKdmirYnn0bY9vKG:oIHj1cYFvKEyFTFOtNVA{UCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwM{Ggcm0> M37KXHNCVkeHUh?=
human NCI-H810 cell NGDTN2hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWHJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KOEGwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{OjJizszN MnjqV2FPT0WU
human HUTU-80 cell NIji[oJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHztflBKdmirYnn0bY9vKG:oIHj1cYFvKEiXVGWtPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN{ODFOwG0> MXzTRW5ITVJ?
human NCI-H460 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M37BdmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi0OlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN|MzFOwG0> M{jRdnNCVkeHUh?=
human HGC-27 cell NGTiSlhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn;wTY5pcWKrdHnvckBw\iCqdX3hckBJT0NvMkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM{QCEQvF2= NY\mRlNDW0GQR1XS
human HSC-2 cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYjxOZhSUW6qaXLpeIlwdiCxZjDoeY1idiCKU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzZ|IH7N MkfrV2FPT0WU
human IA-LM cell M4rPTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHnr[pdKdmirYnn0bY9vKG:oIHj1cYFvKEmDLVzNJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{QTNizszN M121fXNCVkeHUh?=
human HMV-II cell M{Xycmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn73TY5pcWKrdHnvckBw\iCqdX3hckBJVVZvSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM6PCEQvF2= M33LZXNCVkeHUh?=
human COLO-679 cell NV\QNHd[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWrnVWRKUW6qaXLpeIlwdiCxZjDoeY1idiCFT1zPMVY4QSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwM{m0JO69VQ>? NULqVpd4W0GQR1XS
human NCI-H2122 cell NISzZ|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml25TY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKxNlIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjRzIN88US=> MXjTRW5ITVJ?
human CAL-39 cell MnS4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmPkTY5pcWKrdHnvckBw\iCqdX3hckBESUxvM{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQyOSEQvF2= MnnuV2FPT0WU
human SW954 cell NV\nUG1qT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml64TY5pcWKrdHnvckBw\iCqdX3hckBUXzl3NDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOFM3KM7:TR?= MVTTRW5ITVJ?
human LCLC-97TM1 cell M4O1fmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWrJcohq[mm2aX;uJI9nKGi3bXHuJGxEVENvOUfUUVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjR2NDFOwG0> M3PLb3NCVkeHUh?=
human KYSE-270 cell M3PTemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{jJT2lvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT2yO|Ah[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjR2NTFOwG0> NH\XV|VUSU6JRWK=
human NB13 cell M4ixTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXv6THF3UW6qaXLpeIlwdiCxZjDoeY1idiCQQkGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE41PDdizszN NHXqWJFUSU6JRWK=
human SK-LMS-1 cell NEDVd5dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWD5PZB7UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MUXMuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNkS2JO69VQ>? NUfoRnU{W0GQR1XS
human SW620 cell M1nHS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUPJcohq[mm2aX;uJI9nKGi3bXHuJHNYPjJyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD62OFgh|ryP M2\UPXNCVkeHUh?=
human HCT-15 cell MluyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV3ZeZlQUW6qaXLpeIlwdiCxZjDoeY1idiCKQ2StNVUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjdzNzFOwG0> NYPlSJhNW0GQR1XS
human DU-145 cell M13Remdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEjLbGNKdmirYnn0bY9vKG:oIHj1cYFvKESXLUG0OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPzJzIN88US=> M4HxbXNCVkeHUh?=
human ST486 cell NHu4[5pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4np[GlvcGmkaYTpc44hd2ZiaIXtZY4hW1R2OE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlczOSEQvF2= NFrrbJhUSU6JRWK=
human A427 cell NVe2XWcyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlzJTY5pcWKrdHnvckBw\iCqdX3hckBCPDJ5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD64Nlgh|ryP NILVOWRUSU6JRWK=
human HT-1080 cell NGPqcGxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3LYd2lvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMUC4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDR{IN88US=> M{XBc3NCVkeHUh?=
human NCI-H2009 cell Ml\DS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2fNcGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFA6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56NkKg{txO NWHGOZJzW0GQR1XS
human SK-LU-1 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUTZN4t[UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56Nk[g{txO NYHGb4VPW0GQR1XS
human SCC-4 cell Mk\DS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXnJcohq[mm2aX;uJI9nKGi3bXHuJHNESy12IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD64O|ch|ryP NHTQW3FUSU6JRWK=
human NCI-H1299 cell NXHr[YNzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4\WcGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixNlk6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56ODFOwG0> NIjEcm9USU6JRWK=
human NH-12 cell NHjRdItIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M17ZT2lvcGmkaYTpc44hd2ZiaIXtZY4hVkhvMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg6OSEQvF2= M{DGTXNCVkeHUh?=
human SK-UT-1 cell NIC0fZhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MU\Jcohq[mm2aX;uJI9nKGi3bXHuJHNMNVWWLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg6OiEQvF2= MV\TRW5ITVJ?
human Bel7402 MorCVJJwdGmoZYLheIlwdiCjc4PhfS=> M1X4TVczKGh? NYLYU4tnSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB{IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJiR3zvJIF{e2G7LDDJR|UxRTBwOTFOwG0> MV:yNlMzODN3NB?=
human A388 cell NGLzW4xIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkTwTY5pcWKrdHnvckBw\iCqdX3hckBCOzh6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD65OEDPxE1? NIHifmRUSU6JRWK=
human SW982 cell MkCxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYjJcohq[mm2aX;uJI9nKGi3bXHuJHNYQTh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD65PVkh|ryP M4LKWnNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo In a mouse xenograft model of RPMI 8226 cells, Epothilone B (2.5 mg/kg–4 mg/kg) prolongs survival and suppresses tumor growth. [4] Similarly, in mouse xenograft models of prostate cancer cells, including DU145 and PC3, Epothilone B at the same dose also inhibits tumor growth. [6]

Protocol

Kinase Assay:[1]
+ Expand

Tubulin polymerization assay:

Calf brain microtubule proteins (MTP) are purified, which includes approximately 15%–20% microtubule associated proteins. The buffer (MES buffer) used for the Epothilone B-microtubule studies contains 0.1 M 2-morpholinoethanesulfonic acid (MES), 1 mM EGTA, 0.5 mM MgCl2, and 3 M glycerol at pH 6.6. Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone B is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope and expressed as the mean with standard deviation obtained from three different concentrations.
Cell Research:[2]
+ Expand
  • Cell lines: KB3-1, KBV-1, Hela, and Hs578T cells
  • Concentrations: 0–1 μM
  • Incubation Time: 72 hours
  • Method: For mitotic block and aberrant mitosis, cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips. After 24 hours, cells are treated with Epothilone B and scored at regular intervals. For the cytotoxicity analysis, cells are counted and scored as trypan blue positive or negative. Concurrently, coverslips andaliquots of cells in the culture supernatant are fixed and stained with Hoechst33342 in PBS. These cells are scored for cells blocked at the G2/M transition and aberrant mitosis.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Mice xenograft model of RPMI 8226 cells
  • Formulation: Dissolved in30% PEG-300
  • Dosages: 2.5 mg/kg–4 mg/kg
  • Administration: Inject intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 102 mg/mL (200.91 mM)
Ethanol 102 mg/mL (200.91 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.68
Formula

C27H41NO6S

CAS No. 152044-54-7
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00715013 Completed Recurrent Glioblastoma Planned for Reoperation University of Zurich July 2008 Phase 1|Phase 2
NCT00715013 Completed Recurrent Glioblastoma Planned for Reoperation University of Zurich July 2008 Phase 1|Phase 2
NCT00496600 Completed Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers The State University of New Jersey July 2007 Phase 1
NCT00442741 Withdrawn Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1
NCT00496600 Completed Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers The State University of New Jersey July 2007 Phase 1
NCT00442741 Withdrawn Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Microtubule Associated Signaling Pathway Map

Related Microtubule Associated Products0

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID