Home Cytoskeletal Signaling Microtubule Associated inhibitor iHAP1

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iHAP1 Microtubule Associated inhibitor

Cat.No.S9695

iHAP1 is a well-established microtubule poison, blocks microtubule polymerization fully at 5 µM which is independent of deregulated transcription, causes marked sensitization when deleting Rod1 gene in HeLa cells.
iHAP1 Microtubule Associated inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 367.85

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Quality Control

Batch: S969501 DMSO]20 mg/mL]false]Ethanol]5 mg/mL]false]Water]Insoluble]false Purity: 99.84%
99.84

Chemical Information, Storage & Stability

Molecular Weight 367.85 Formula

C20H14ClNO2S

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 105925-39-1 -- Storage of Stock Solutions

Synonyms N/A Smiles COC1=CC=C(C=C1)C(=O)N2C3=CC=CC=C3SC4=C2C=C(Cl)C=C4

Solubility

In vitro
Batch:

DMSO : 20 mg/mL ( (54.36 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Mechanism of Action

Targets/IC50/Ki
microtubule polymerization [1]
In vitro

The depletion of Rod1 and TACC3 results in sensitization to iHAP1, which is caused by chemogenic interaction between Rod1 and this compound. As for the RPE1‐hTERT P53−/− background, deletion of Rod1 in HeLa cells causes marked sensitization to this chemical.[1]
References

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