Nocodazole

Catalog No.S2775 Synonyms: Oncodazole

Nocodazole Chemical Structure

Molecular Weight(MW): 301.32

Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.

Size Price Stock Quantity  
USD 70 In stock
USD 270 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

2 Customer Reviews

  • A, HeLa cells were treated with DMSO, Taxol (100 nM for 16 h), or Nocodazole (Noco, 100 ng/ml for 16 h). Total cell lysates were probed with the indicated antibodies against Hippo components on Phos-tag SDS-polyacrylamide gels. O and * mark the non-phosphorylated and phosphorylated proteins, respectively.

    J Biol Chem, 2016, 291:14761-14772.. Nocodazole purchased from Selleck.

    (B) HeLa cells were treated with DMSO, Taxol or Nocodazole (Noco). Total cell lysates were probed with the indicated antibodies on Phos-tag or regular SDS-polyacrylamide gels.

    Cell Signal, 2016, 28(12):1826-1832. Nocodazole purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.
Targets
Microtubules [2]
(Cell-free assay)
Abl [1]
(Cell-free assay)
Abl (E255K) [1]
(Cell-free assay)
Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
In vitro

Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. [1] At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. [2] Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. [3] Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. [4]Nocodazole doubles HDR efficiency to up to 30% in iPSCs. It improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing[6].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat LT12 cells MUfQdo9tcW[ncnH0bY9vKGG|c3H5 M{\mUFQ5KGh? NHTQVlFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JJJifCCOVEGyJINmdGy|IHHmeIVzKDR6IHjyd{BjgSC[VGSgZZN{[XluIFnDOVA:PiCwTR?= MWSxPVIzODBzOB?=
human PANC1 cells Mne4VJJwdGmoZYLheIlwdiCjc4PhfS=> M2no[FQ5KGh? MofmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCSQV7DNUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTFyIH7N MlfCNlU5Ojd3MkK=
human HeLa cells NX3nfXY6WHKxbHnm[ZJifGmxbjDhd5NigQ>? M3P5SFQ5KGh? NFvRZVdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygS2k2OD1zNTDuUS=> M4PScFI2QDJ5NUKy
human MDA-MB-231 cells MV7Qdo9tcW[ncnH0bY9vKGG|c3H5 NWPLO|VjPDhiaB?= NHHrcHZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF0yPyCwTR?= NGLJSVMzPTh{N{WyNi=>
human A549 cells NH70eoRRem:uaX\ldoF1cW:wIHHzd4F6 NEPzPYU1QCCq NH7F[XpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygS2k2OD1{MDDuUS=> MkjnNlU5Ojd3MkK=
lymphoid leukemia L1210 cells NX7aO3o6WHKxbHnm[ZJifGmxbjDhd5NigQ>? MX:0PEBp MWfJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCycn;sbYZmemG2aX;uJI9nKGO3bIT1doVlKGy7bYDoc4llKGyndXvlcYliKExzMkGwJINmdGy|IHT1dolv\yB2ODDodpMtKEmGNUC9Nlchdk1? M37aN|cyOzF2OEO=
human A549 cells  NIfabItEgXSxdH;4bYPDqGG|c3H5 MXqyOEBp NFOzNVFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9N|YvPiCwTR?= NYeyc3Z7OjBzMUCxN|c>
L1210 murine leukemia cell NVXVOIxvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mnz1TY4hfmm2cn:g[o9zKGm2czDpcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgUFEzOTBibYXybY5mKGyndXvlcYliKGOnbHyg[5Jwf3SqLDDJR|UxRTN6IH7N NEH0ZXgyPzN6MUS2
mouse P388 cells NE[ybVlRem:uaX\ldoF1cW:wIHHzd4F6 NHrxO441QCCq MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKFB|OEigZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFiWVDDhd5NigSxiSVO1NF01OCCwTR?= NECzNlUyQTJ{MECxPC=>
LT12 cells Mmm1VJJwdGmoZYLheIlwdiCjc4PhfS=> NGHUXW81QCCq MV;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFzUNVIh[2WubIOgZpkhYFSWIHHzd4F6KGGodHXyJFQ5KGi{czygTWM2OD12MDDuUS=> MlHJNVcyQDFzNkS=
human U2OS cells MnzISpVv[3Srb36gZZN{[Xl? NFzXfJc4OiCq MmjwTY5lfWO2aX;uJI9nKG2rdH;0bYMh[XK{ZYP0JIlvKGi3bXHuJHUzV1NiY3XscJMh[XO|ZYPz[YQh[XNibXn0c5Rq[yCrbnTlfEBi\nSncjC3NkBpenNiYomgcYlkem:|Y3;wbYMh[W6jbInzbZMtKEWFNUC9OVAhdk1? M2HGc|I1QTByOEi3
human NCI-H460 cells M4\kR3Bzd2yrZnXyZZRqd25iYYPzZZk> MkPVOFghcA>? M3TJfGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJMWg1PjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPU[4JI5O M4HLVVIyPTZ|N{Ww
human BT549 cells MWrQdo9tcW[ncnH0bY9vKGG|c3H5 MUG0PEBp M3r6S2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS1OFkh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTZ7IH7N NHX2d2QzOTV4M{e1NC=>
human 451LU cells NIP5O|FRem:uaX\ldoF1cW:wIHHzd4F6 NFey[ZY1QCCq NI\SVVdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIES1NWxWKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OD15MjDuUS=> NIT2Z3gzOTV4M{e1NC=>
human SKOV3 cells NI\IPW5Rem:uaX\ldoF1cW:wIHHzd4F6 NEPtNoc1QCCq MlroRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVS1;WN{Bk\WyuczDh[pRmeiB2ODDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFnDOVA:QDBibl2= M1[xW|IyPTZ|N{Ww
K562 cells NXzpTYFqTnWwY4Tpc44h[XO|YYm= M1vzWWVn\mWldHn2[UBkd26lZX70doF1cW:wIILldZVqemWmIITvJIFzemW|dDDwdo9xcWSrdX2gbY9lcWSnIIP0ZYlv\WRiS{W2NkBk\WyuczDpckBIOi:PIIDoZZNmNCCHQ{WwQVgzKG6P NWe1fGNOOTJ6NUK3Olg>
human SKBR3 cells MonNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVTUVoZxPDhiaB?= NXzYVGpYT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hW0uEUkOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigQ>? MV6yNlg2ODJzNB?=
human COLO205 cells NWHrWVMyWHKxbHnm[ZJifGmxbjDhd5NigQ>? MmT4OFghcA>? MmDqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCFT1zPNlA2KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTCsJGlEPTB;OEigcm0> M4DKUFIyPTZ|N{Ww
human KB/HeLa cells MVfGeY5kfGmxbjDhd5NigQ>? MXmyOEBp NG\R[2dE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBMSi:KZVzhJINmdGy|IHHzd4V{e2WmIHHzJGczN01icHjhd4Uh[WOldX31cIF1cW:wIHHmeIVzKDJ2IHjyd{BjgSCIQVPTJIFv[Wy7c3nzMEBGSzVyPUmxJI5O M2L3W|E6OjJyMEG4
human A549 cells Mo\iS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGDQTIZIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBCPTR7IHPlcIx{KGK7IGP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUC9NE4yKM7:TR?= Mn65NlEyODZ2NUi=
human MCF7 cells M33MVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEj1WlRIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOS0Z5IHPlcIx{KGK7IGP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUC9NE4yKM7:TR?= NWr4dI1GOjFzME[0OVg>
human RKO cells M1z5UnBzd2yrZnXyZZRqd25iYYPzZZk> MnW2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCUS1:gZ4VtdHNiYomgXHRVKGG|c3H5MEBKSzVyPUCuNVEh|ryP M1y5RVE6OjJyMEG4
human SW480 cells NIHIbpZRem:uaX\ldoF1cW:wIHHzd4F6 MVK0PEBp MmrwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{S4NEBk\WyuczDh[pRmeiB2ODDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFnDOVA:OC5zMjFOwG0> NYTUV|VROjF3NkO3OVA>
human NCI-H460 cells NI\KUYlRem:uaX\ldoF1cW:wIHHzd4F6 NH7Mb4U1QCCq MmnhRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCQQ1mtTFQ3OCClZXzsd{Bi\nSncjC0PEBpenNiYomgXHRVKGG|c3H5MEBKSzVyPUCuNVUh|ryP MUWyNFU{Pzd4NR?=
human SF268 cells Mn63R5l1d3SxeHnjxsBie3OjeR?= NFPuV2w1QCCq Mo\jR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2YzPjhiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHhVXCCjc4PhfUwhUUN3ME2wMlMh|ryP MVOyNVcxPTJ{Mx?=
human SK-N-SH cells M4LSb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkTyS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV2suVi2VSDDj[YxteyCkeTDTeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvPjZizszN MUKyNVExPjR3OB?=
human MIAPaCa2 cells Ml3JVJJwdGmoZYLheIlwdiCjc4PhfS=> MlnROFghcA>? NFW4PIhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3JRXBiS2F{IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OC57NTFOwG0> M4i5flI2OTNzOUW2
human HaCaT cells NGTYZoVEgXSxdH;4bYPDqGG|c3H5 MnnHOFghcA>? MYHDfZRwfG:6aXOgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKYVPhWEBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEBoem:5dHigbY5kfWKjdHXkJIZweiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRThwOTFOwG0> MojaNlYzOzFyOEC=
human SGC7901 cells NFnWS3lHfW6ldHnvckBie3OjeR?= NV7JWI4yPDhiaB?= MonPTY5lfWO2aX;uJI9nKGSrc4TvdpRqd26|IHnuJJR2[nWuaX6gZZN{\W2kbImgbY4hcHWvYX6gV2dEPzlyMTDj[YxteyCjc4Pld5Nm\CCjczDhZo5wem2jbDDtbZRwfGmlIIPwbY5ldGViZn;ycYF1cW:wIHH0JFIh\m:uZDDJR|UxKGyndnXsJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IHntcZVvd2[udX;y[ZNk\W6lZTDzeIFqdmmwZzDiZZNm\CCobIXvdoV{[2WwY3WgcYlkem:|Y3;wfS=> M2nEOlI2OjZ{MEWx
human Jurkat T cells NEPMXWVHfW6ldHnvckBie3OjeR?= Mk\MN|YhcA>? MUTJcoR2[3Srb36gc4YhdWmlcn;0eYJ2dGViZHHtZYdmKGmwIHj1cYFvKEq3cnvheEBVKGOnbHzzJI93\XKneIDy[ZN{cW6pIFLjcFIh[W[2ZYKgN|YhcHK|IHL5JIludXWwb4P0ZYlvcW6pIH3leIhw\A>? M2nINlIzOTl5M{mz
human PC3M cells NFvaVGpHfW6ldHnvckBie3OjeR?= NHPHT5YxNjVidXevcYw> MkXHNlQhcA>? MWDJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKFCFM12gZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IIP1ZmcyKHCqYYPlJIF1KDBwNTD1[{9udCCjZoTldkAzPCCqcoOgZpkh\myxdzDjfZRwdWW2com= MYKxPFI1PzV5Mx?=
mouse NIH3T3 cells M3LXdWZ2dmO2aX;uJIF{e2G7 NFHObJExNjVidXevcYw> MVmyOEBp MYPJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIH3veZNmKE6LSEPUN{Bk\WyuczDhd5Nme3OnZDDhd{Bi[2O3bYXsZZRqd25iYYSgd5VjTzFicHjhd4Uh[XRiMD61JJVoN22uIHHmeIVzKDJ2IHjyd{BjgSCobH;3JIN6fG:vZYTyfS=> MWCxPFI1PzV5Mx?=

... Click to View More Cell Line Experimental Data

In vivo The tumor volume and tumor weight of the mice treated with Ketoconazole  plus  Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole  plus  Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole  or  Nocodazole alone. [5]

Protocol

Animal Research:

[5]

+ Expand
  • Animal Models: Nude mice with COLO-205 tumor xenografts
  • Formulation: 0.05% dimethyls
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 7 mg/mL (23.23 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 301.32
Formula

C14H11N3O3S

CAS No. 31430-18-9
Storage powder
in solvent
Synonyms Oncodazole

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Microtubule Associated Signaling Pathway Map

Related Microtubule Associated Products

Tags: buy Nocodazole | Nocodazole supplier | purchase Nocodazole | Nocodazole cost | Nocodazole manufacturer | order Nocodazole | Nocodazole distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID