Nocodazole

For research use only.

Catalog No.S2775 Synonyms: Oncodazole

41 publications

Nocodazole Chemical Structure

Molecular Weight(MW): 301.32

Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole induces apoptosis.

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Selleck's Nocodazole has been cited by 41 publications

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Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole induces apoptosis.
Targets
Microtubules [2]
(Cell-free assay)
Abl [1]
(Cell-free assay)
Abl (E255K) [1]
(Cell-free assay)
Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
In vitro

Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. [1] At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. [2] Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. [3] Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. [4]Nocodazole doubles HDR efficiency to up to 30% in iPSCs. It improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing[6].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat LT12 cells NGXlUmlRem:uaX\ldoF1cW:wIHHzd4F6 NH7VfWo1QCCq NVnkUnlESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBz[XRiTGSxNkBk\WyuczDh[pRmeiB2ODDodpMh[nliWGTUJIF{e2G7LDDJR|UxRTZibl2= MVWxPVIzODBzOB?=
human PANC1 cells MoDpVJJwdGmoZYLheIlwdiCjc4PhfS=> M4TqUVQ5KGh? MlPpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCSQV7DNUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTFyIH7N NHy0T4YzPTh{N{WyNi=>
human HeLa cells NVnobG5[WHKxbHnm[ZJifGmxbjDhd5NigQ>? NWLDZ5B7PDhiaB?= M33sSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXMZUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTF3IH7N M4\GTlI2QDJ5NUKy
human MDA-MB-231 cells M4TFTnBzd2yrZnXyZZRqd25iYYPzZZk> NI\Pb3Y1QCCq MmHiRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEeLNUC9NVchdk1? NFfReI8zPTh{N{WyNi=>
human A549 cells M3P0UHBzd2yrZnXyZZRqd25iYYPzZZk> MmrDOFghcA>? NX;2RY43SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGdKPTB;MkCgcm0> MkDaNlU5Ojd3MkK=
lymphoid leukemia L1210 cells MU\Qdo9tcW[ncnH0bY9vKGG|c3H5 MoK1OFghcA>? NVnVe4tvUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDjeYx1fXKnZDDsfY1xcG:rZDDs[ZVs\W2rYTDMNVIyOCClZXzsd{BlfXKrbnegOFghcHK|LDDJSFUxRTJ5IH7N NI\4T2g4OTNzNEiz
human A549 cells  MlX0R5l1d3SxeHnjxsBie3OjeR?= NXPpfJJtOjRiaB?= M2C4WGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2zOk43KG6P MorrNlAyOTBzM{e=
L1210 murine leukemia cell M4rNVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVnJckB3cXS{bzDmc5IhcXS|IHnubIljcXSxcomgZYN1cX[rdImgZYdicW6|dDDMNVIyOCCvdYLpcoUhdGW3a3XtbYEh[2WubDDndo94fGhuIFnDOVA:Ozhibl2= M3G3bFE4OzhzNE[=
mouse P388 cells MoLtVJJwdGmoZYLheIlwdiCjc4PhfS=> M{fH[lQ5KGh? NF;pWldCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIGCzPFgh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGjUWEBie3OjeTygTWM2OD12MDDuUS=> NXLlOnE3OTl{MkCwNVg>
LT12 cells NYTz[ZZNWHKxbHnm[ZJifGmxbjDhd5NigQ>? MVy0PEBp M2LuVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgUHQyOiClZXzsd{BjgSC[VGSgZZN{[XliYX\0[ZIhPDhiaILzMEBKSzVyPUSwJI5O MY[xO|E5OTF4NB?=
human U2OS cells NIW5b|RHfW6ldHnvckBie3OjeR?= NUXFVFlPPzJiaB?= M2f6V2lv\HWldHnvckBw\iCvaYTveIlkKGG{cnXzeEBqdiCqdX3hckBWOk:VIHPlcIx{KGG|c3Xzd4VlKGG|IH3peI91cWNiaX7k[Zgh[W[2ZYKgO|IhcHK|IHL5JI1q[3Kxc3PvdIlkKGGwYXz5d4l{NCCHQ{WwQVUxKG6P MUSyOFkxODh6Nx?=
human NCI-H460 cells NFPP[25Rem:uaX\ldoF1cW:wIHHzd4F6 NGXSPJc1QCCq NH7rNpBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF7DTU1JPDZyIHPlcIx{KGGodHXyJFQ5KGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME22PEBvVQ>? M3q5VlIyPTZ|N{Ww
human BT549 cells NXX4bIdkWHKxbHnm[ZJifGmxbjDhd5NigQ>? NEnhOmc1QCCq NEW4[llCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLUOVQ6KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OD14OTDuUS=> MoPGNlE2PjN5NUC=
human 451LU cells M{DRR3Bzd2yrZnXyZZRqd25iYYPzZZk> MoPZOFghcA>? M1HLfGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iNEWxUHUh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTd{IH7N MUOyNVU3Ozd3MB?=
human SKOV3 cells NEe1ZYpRem:uaX\ldoF1cW:wIHHzd4F6 MVe0PEBp M3f4WWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1vPWlMh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRThyIH7N NWnSSoV5OjF3NkO3OVA>
K562 cells MXfGeY5kfGmxbjDhd5NigQ>? M4jKVGVn\mWldHn2[UBkd26lZX70doF1cW:wIILldZVqemWmIITvJIFzemW|dDDwdo9xcWSrdX2gbY9lcWSnIIP0ZYlv\WRiS{W2NkBk\WyuczDpckBIOi:PIIDoZZNmNCCHQ{WwQVgzKG6P MnPRNVI5PTJ5Nki=
human SKBR3 cells NX\1RZRmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIfRXXE1QCCq NWXIU49zT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hW0uEUkOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigQ>? NYLWd3JUOjJ6NUCyNVQ>
human COLO205 cells MnHkVJJwdGmoZYLheIlwdiCjc4PhfS=> NITGNY01QCCq M1;ZTGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ1;MU|IxPSClZXzsd{Bi\nSncjC0PEBpenNiYomgZYxidWG{IHLseYUh[XO|YYmgMEBKSzVyPUi4JI5O MlX2NlE2PjN5NUC=
human KB/HeLa cells NIXNOnRHfW6ldHnvckBie3OjeR?= NFnMUIEzPCCq NV\Yb|VoS2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hU0JxSHXMZUBk\WyuczDhd5Nme3OnZDDhd{BIOi:PIIDoZZNmKGGlY4XteYxifGmxbjDh[pRmeiB{NDDodpMh[nliRlHDV{BidmGueYPpd{whTUN3ME25NUBvVQ>? NFLnbnUyQTJ{MECxPC=>
human A549 cells MnLVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWi3OGFKT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hSTV2OTDj[YxteyCkeTDTeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvOSEQvF2= M3PSXVIyOTB4NEW4
human MCF7 cells MkXWS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4r3dWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscJMh[nliU4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhT0l3ME2wMlEh|ryP MmjINlEyODZ2NUi=
human RKO cells NYq2TIkxWHKxbHnm[ZJifGmxbjDhd5NigQ>? NV;VTGJZSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDST28h[2WubIOgZpkhYFSWIHHzd4F6NCCLQ{WwQVAvOTFizszN M1O0NFE6OjJyMEG4
human SW480 cells NVzXXXl[WHKxbHnm[ZJifGmxbjDhd5NigQ>? MUi0PEBp Mn\URY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{S4NEBk\WyuczDh[pRmeiB2ODDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFnDOVA:OC5zMjFOwG0> MmOzNlE2PjN5NUC=
human NCI-H460 cells NXfIb|h7WHKxbHnm[ZJifGmxbjDhd5NigQ>? NV2yOIdvPDhiaB?= MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3IOFYxKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDYWHQh[XO|YYmsJGlEPTB;MD6xOUDPxE1? MnW0NlA2Ozd5NkW=
human SF268 cells MX7DfZRwfG:6aXRCpIF{e2G7 M3TlPVQ5KGh? M3OzZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNHOjZ6IHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUC9NE4{KM7:TR?= NEXOZm0zOTdyNUKyNy=>
human SK-N-SH cells NX35SndnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTT{1PNVOKIHPlcIx{KGK7IGP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUC9NE43PiEQvF2= NVnMUVFROjFzME[0OVg>
human MIAPaCa2 cells MmnyVJJwdGmoZYLheIlwdiCjc4PhfS=> NGTrWmE1QCCq NHvudWZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3JRXBiS2F{IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OC57NTFOwG0> NEHsUWgzPTF|MUm1Oi=>
human HaCaT cells NF7iO3hEgXSxdH;4bYPDqGG|c3H5 NHTvdJQ1QCCq NXe3UIl2S3m2b4TvfIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTIFE[VRiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxiZ4Lve5RpKGmwY4XiZZRm\CCob4KgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME24Mlkh|ryP NF;keIkzPjJ|MUC4NC=>
human SGC7901 cells MnfMSpVv[3Srb36gZZN{[Xl? M2DrU|Q5KGh? MnrsTY5lfWO2aX;uJI9nKGSrc4TvdpRqd26|IHnuJJR2[nWuaX6gZZN{\W2kbImgbY4hcHWvYX6gV2dEPzlyMTDj[YxteyCjc4Pld5Nm\CCjczDhZo5wem2jbDDtbZRwfGmlIIPwbY5ldGViZn;ycYF1cW:wIHH0JFIh\m:uZDDJR|UxKGyndnXsJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IHntcZVvd2[udX;y[ZNk\W6lZTDzeIFqdmmwZzDiZZNm\CCobIXvdoV{[2WwY3WgcYlkem:|Y3;wfS=> MVSyOVI3OjB3MR?=
human Jurkat T cells M2C0XGZ2dmO2aX;uJIF{e2G7 NYjGdJBkOzZiaB?= MlHqTY5lfWO2aX;uJI9nKG2rY4LveJVjfWynIHThcYFo\SCrbjDoeY1idiCMdYLrZZQhXCClZXzsd{BwfmW{ZYjwdoV{e2mwZzDCZ4wzKGGodHXyJFM3KGi{czDifUBqdW23bn;zeIFqdmmwZzDt[ZRpd2R? MmLWNlIyQTd|OUO=
human PC3M cells NXHZd4dETnWwY4Tpc44h[XO|YYm= NFLTPYIxNjVidXevcYw> NH7IPY8zPCCq Mnz2TY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCSQ{PNJINmdGy|IHHzd4V{e2WmIHHzJIFk[3WvdXzheIlwdiCjdDDzeYJIOSCyaHHz[UBifCByLkWgeYcwdWxiYX\0[ZIhOjRiaILzJIJ6KG[ub4egZ5l1d22ndIL5 NVraS4xyOTh{NEe1O|M>
mouse NIH3T3 cells MU\GeY5kfGmxbjDhd5NigQ>? M{THUlAvPSC3Zz;tcC=> M4TJdFI1KGh? NUTqUHNrUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBud3W|ZTDOTWg{XDNiY3XscJMh[XO|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJIF1KHO3YlexJJBp[XOnIHH0JFAvPSC3Zz;tcEBi\nSncjCyOEBpenNiYomg[oxwfyCleYTvcYV1enl? M3G4WVE5OjR5NUez

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cdc2 / cyclin B1 / MAD2 / Cdc20 ; 

PubMed: 21918689     


E (upper part). Time-dependent changes in cyclin B1 and Cdc2 protein levels following nocodazole treatment. Cells were treated with nocodazole (250 nM) for the length of time as indicated, and whole cell lysates were prepared. An equal amount of protein lysates was electrophoretically separated on the 10% SDS-polyacrylamide gel, and transferred to nitrocellulose membrane. Western blots were detected using specific antibodies against cyclin B1, Cdc2 (CDK1), MAD2, and Cdc20 on an enhanced chemiluminescence (ECL) apparatus. Membrane was stripped for determining the levels of GAPDH as a loading control. E (lower part). The relative protein levels for cyclin B1, Cdc2, MAD2, and Cdc20 were calculated according to their densitometry readings, which were normalized according to the corresponding readings for the GAPDH protein bands. Each value is mean ± S.D. from three replicate determinations. * P<0.05, ** P<0.01 versus vehicle-treated control. 

21918689
Immunofluorescence
Tubulin / LC8 ; 

PubMed: 23038268     


Shown is endogenous LC8 colocalized with microtubules in HeLa cells. Control, 20 nm taxol- and 100 nm nocodazole-treated cells were stained with α-tubulin and LC8 antibodies. Scale bar, 20 μm.

Brd4 / α-tubulin ; 

PubMed: 22567088     


Cells were treated with indicated concentrations of nocodazole for 4 h, and mitotic cells were immunostained for endogenous Brd4 and α-tubulin. DNA was stained by Hoechst33324.

23038268 22567088
Growth inhibition assay
Cell viability ; 

PubMed: 21918689     


Changes in cell viability (MTT assay) after treatment of cells with 250 nM nocodazole for different lengths of time. Each data point is the mean ± S.D. from four replicate measurements from one representative experiment. 

21918689
In vivo The tumor volume and tumor weight of the mice treated with Ketoconazole  plus  Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole  plus  Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole  or  Nocodazole alone. [5]

Protocol

Animal Research:

[5]

- Collapse
  • Animal Models: Nude mice with COLO-205 tumor xenografts
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 7 mg/mL (23.23 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 301.32
Formula

C14H11N3O3S

CAS No. 31430-18-9
Storage powder
in solvent
Synonyms Oncodazole
Smiles COC(=O)NC1=NC2=CC=C(C=C2[NH]1)C(=O)C3=CC=CS3

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Microtubule Associated Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID