Nocodazole

Catalog No.S2775 Synonyms: Oncodazole

Nocodazole Chemical Structure

Molecular Weight(MW): 301.32

Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.

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Cited by 20 Publications

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Biological Activity

Description Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.
Targets
Microtubules [2]
(Cell-free assay)
Abl [1]
(Cell-free assay)
Abl (E255K) [1]
(Cell-free assay)
Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
In vitro

Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. [1] At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. [2] Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. [3] Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. [4]Nocodazole doubles HDR efficiency to up to 30% in iPSCs. It improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing[6].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat LT12 cells MljCVJJwdGmoZYLheIlwdiCjc4PhfS=> MoXnOFghcA>? MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IILheEBNXDF{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUC9OkBvVQ>? MoXwNVkzOjByMUi=
human PANC1 cells NVvkbmFEWHKxbHnm[ZJifGmxbjDhd5NigQ>? NEjKXXU1QCCq NXfQV|BlSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQRW5EOSClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBIUTVyPUGwJI5O M1;UdFI2QDJ5NUKy
human HeLa cells M2rpTXBzd2yrZnXyZZRqd25iYYPzZZk> MmHpOFghcA>? MoKxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OTVibl2= MoDDNlU5Ojd3MkK=
human MDA-MB-231 cells M2GyfXBzd2yrZnXyZZRqd25iYYPzZZk> MkLVOFghcA>? NGPHSIhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF0yPyCwTR?= M1i5UlI2QDJ5NUKy
human A549 cells M{nFU3Bzd2yrZnXyZZRqd25iYYPzZZk> MXW0PEBp M2jkd2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTJyIH7N Mn[5NlU5Ojd3MkK=
lymphoid leukemia L1210 cells NF3kS4hRem:uaX\ldoF1cW:wIHHzd4F6 NF3wUI41QCCq MkLWTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RicILvcIln\XKjdHnvckBw\iCldXz0eZJm\CCueX3wbI9q\CCuZYXr[Y1q[SCOMUKxNEBk\WyuczDkeZJqdmdiNEigbJJ{NCCLREWwQVI4KG6P MlryO|E{OTR6Mx?=
human A549 cells  NW[zeXBMS3m2b4TvfIlkyqCjc4PhfS=> MlTPNlQhcA>? MlXFR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUO2MlYhdk1? MnjrNlAyOTBzM{e=
L1210 murine leukemia cell MknnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFP3eZpKdiC4aYTyc{Bnd3JiaYTzJIlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBNOTJzMDDteZJqdmVibHX1b4VucWFiY3XscEBoem:5dHisJGlEPTB;M{igcm0> M2nyfVE4OzhzNE[=
mouse P388 cells MmLFVJJwdGmoZYLheIlwdiCjc4PhfS=> NVG1[3dNPDhiaB?= NEHm[2xCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIGCzPFgh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGjUWEBie3OjeTygTWM2OD12MDDuUS=> NH\xVYcyQTJ{MECxPC=>
LT12 cells NIjobI5Rem:uaX\ldoF1cW:wIHHzd4F6 M2rhZlQ5KGh? NGj2TG1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGxVOTJiY3XscJMh[nliWGTUJIF{e2G7IHHmeIVzKDR6IHjyd{whUUN3ME20NEBvVQ>? MU[xO|E5OTF4NB?=
human U2OS cells Moi3SpVv[3Srb36gZZN{[Xl? MljDO|IhcA>? MXnJcoR2[3Srb36gc4YhdWm2b4TpZ{BienKnc4SgbY4hcHWvYX6gWVJQWyClZXzsd{Bie3Onc4Pl[EBieyCvaYTveIlkKGmwZHX4JIFnfGW{IEeyJIhzeyCkeTDtbYNzd3Olb4DpZ{BidmGueYPpd{whTUN3ME21NEBvVQ>? NVny[4tHOjR7MEC4PFc>
human NCI-H460 cells M1L1W3Bzd2yrZnXyZZRqd25iYYPzZZk> M4XYS|Q5KGh? M{C5[WFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJMWg1PjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPU[4JI5O M{PzbVIyPTZ|N{Ww
human BT549 cells NWTvRXp[WHKxbHnm[ZJifGmxbjDhd5NigQ>? NXjheGZyPDhiaB?= NHXDbWRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLUOVQ6KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OD14OTDuUS=> Ml20NlE2PjN5NUC=
human 451LU cells NFPiN2xRem:uaX\ldoF1cW:wIHHzd4F6 M4X4bFQ5KGh? NV\zVZoySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjC0OVFNXSClZXzsd{Bi\nSncjC0PEBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;N{Kgcm0> MVKyNVU3Ozd3MB?=
human SKOV3 cells NIrrR4lRem:uaX\ldoF1cW:wIHHzd4F6 MlHDOFghcA>? NWq0SWhMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{Bi\nSncjC0PEBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;OECgcm0> MofNNlE2PjN5NUC=
K562 cells NHOwOFZHfW6ldHnvckBie3OjeR?= NV7ZfpdvTW[oZXP0bZZmKGOxbnPlcpRz[XSrb36gdoVyfWm{ZXSgeI8h[XK{ZYP0JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6nZDDLOVYzKGOnbHzzJIlvKEd{L12gdIhie2VuIFXDOVA:QDJibl2= MoXWNVI5PTJ5Nki=
human SKBR3 cells M4Swemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF7Nbpo1QCCq NHzCcmpIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBUU0KUMzDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6 M{naRlIzQDVyMkG0
human COLO205 cells MlLyVJJwdGmoZYLheIlwdiCjc4PhfS=> NFn3[Fg1QCCq NGj4[IRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFPPUG8zODViY3XscJMh[W[2ZYKgOFghcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5JEwhUUN3ME24PEBvVQ>? NEW3[|AzOTV4M{e1NC=>
human KB/HeLa cells M4j5b2Z2dmO2aX;uJIF{e2G7 MXmyOEBp M2e1W2NmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGtDN0inTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgS|IwVSCyaHHz[UBi[2O3bYXsZZRqd25iYX\0[ZIhOjRiaILzJIJ6KE[DQ2OgZY5idHm|aYOsJGVEPTB;OUGgcm0> NXewNndNOTl{MkCwNVg>
human A549 cells NXPUbJh1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX;TOJpnT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hSTV2OTDj[YxteyCkeTDTeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvOSEQvF2= MX2yNVExPjR3OB?=
human MCF7 cells NGnWXXNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV7Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNR2Y4KGOnbHzzJIJ6KFO3bH\vdohw\GGvaX7lJGIh[XO|YYmsJGdKPTB;MD6xJO69VQ>? NULjcppEOjFzME[0OVg>
human RKO cells NYLKR2JNWHKxbHnm[ZJifGmxbjDhd5NigQ>? NGTlXo9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGLLU{Bk\WyuczDifUBZXFRiYYPzZZktKEmFNUC9NE4yOSEQvF2= NWCy[IpMOTl{MkCwNVg>
human SW480 cells NHnUe5BRem:uaX\ldoF1cW:wIHHzd4F6 M{f0WVQ5KGh? M{jI[2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e0PFAh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTBwMUKg{txO MkHkNlE2PjN5NUC=
human NCI-H460 cells NVmzTmJnWHKxbHnm[ZJifGmxbjDhd5NigQ>? NUDvdoNtPDhiaB?= M3nJO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJMWg1PjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHhVXCCjc4PhfUwhUUN3ME2wMlE2KM7:TR?= M{H4TlIxPTN5N{[1
human SF268 cells Ml;rR5l1d3SxeHnjxsBie3OjeR?= NFPVWJM1QCCq NEDWV3pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUTjJ4ODDj[YxteyCjZoTldkA1QCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwQVAvOyEQvF2= MorvNlE4ODV{MkO=
human SK-N-SH cells MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmnYS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV2suVi2VSDDj[YxteyCkeTDTeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvPjZizszN MmfQNlEyODZ2NUi=
human MIAPaCa2 cells NXjMcmhkWHKxbHnm[ZJifGmxbjDhd5NigQ>? M3nPT|Q5KGh? M1fNVWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVnBVIFE[TJiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF0xNjl3IN88US=> NHy2[|kzPTF|MUm1Oi=>
human HaCaT cells MXTDfZRwfG:6aXRCpIF{e2G7 MmL6OFghcA>? NH\YWFlEgXSxdH;4bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIZWNiXCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCCpcn;3eIghcW6ldXLheIVlKG[xcjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUiuPUDPxE1? NWftflBQOjZ{M{GwPFA>
human SGC7901 cells M{TrTWZ2dmO2aX;uJIF{e2G7 NGfld4k1QCCq NF3OeZNKdmS3Y4Tpc44hd2ZiZHnzeI9zfGmxboOgbY4hfHWkdXzpckBie3OnbXLsfUBqdiCqdX3hckBUT0N5OUCxJINmdGy|IHHzd4V{e2WmIHHzJIFjdm:{bXHsJI1qfG:2aXOgd5BqdmSuZTDmc5Ju[XSrb36gZZQhOiCob3zkJGlEPTBibHX2[YwhcW6ldXLheIVlKG[xcjC0PEBpenNiYomgbY1ufW6xZnz1c5Jme2OnbnPlJJN1[WmwaX7nJIJie2WmIH\seY9z\XOlZX7j[UBucWO{b4Pjc5B6 M1\kelI2OjZ{MEWx
human Jurkat T cells MWjGeY5kfGmxbjDhd5NigQ>? NVzsT25EOzZiaB?= MVfJcoR2[3Srb36gc4YhdWmlcn;0eYJ2dGViZHHtZYdmKGmwIHj1cYFvKEq3cnvheEBVKGOnbHzzJI93\XKneIDy[ZN{cW6pIFLjcFIh[W[2ZYKgN|YhcHK|IHL5JIludXWwb4P0ZYlvcW6pIH3leIhw\A>? M17CVlIzOTl5M{mz
human PC3M cells M3q0bWZ2dmO2aX;uJIF{e2G7 MkXkNE42KHWpL33s MVuyOEBp MVnJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKFCFM12gZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IIP1ZmcyKHCqYYPlJIF1KDBwNTD1[{9udCCjZoTldkAzPCCqcoOgZpkh\myxdzDjfZRwdWW2com= NIjPWG0yQDJ2N{W3Ny=>
mouse NIH3T3 cells NHLnbItHfW6ldHnvckBie3OjeR?= NUjKUIQ{OC53IIXnM41t M3n5dVI1KGh? M2HVZWlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gcY92e2ViTlnIN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEB{fWKJMTDwbIF{\SCjdDCwMlUhfWdxbXygZYZ1\XJiMkSgbJJ{KGK7IH\sc5ch[3m2b33leJJ6 MYmxPFI1PzV5Mx?=

... Click to View More Cell Line Experimental Data

In vivo The tumor volume and tumor weight of the mice treated with Ketoconazole  plus  Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole  plus  Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole  or  Nocodazole alone. [5]

Protocol

Animal Research:

[5]

+ Expand
  • Animal Models: Nude mice with COLO-205 tumor xenografts
  • Formulation: 0.05% dimethyls
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 7 mg/mL (23.23 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 301.32
Formula

C14H11N3O3S

CAS No. 31430-18-9
Storage powder
in solvent
Synonyms Oncodazole

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID