Nocodazole

Catalog No.S2775 Synonyms: Oncodazole

Nocodazole Chemical Structure

Molecular Weight(MW): 301.32

Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.

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Cited by 16 Publications

4 Customer Reviews

  • A, HeLa cells were treated with DMSO, Taxol (100 nM for 16 h), or Nocodazole (Noco, 100 ng/ml for 16 h). Total cell lysates were probed with the indicated antibodies against Hippo components on Phos-tag SDS-polyacrylamide gels. O and * mark the non-phosphorylated and phosphorylated proteins, respectively.

    J Biol Chem, 2016, 291:14761-14772.. Nocodazole purchased from Selleck.

  • AURKA and PRL-3's time-scaled distributions in HCT116 were assessed by synchronization and released after serum starvation (SS) for 24 hours or NOC treatment for 12 hours. Arrows, mitotic cells.

    Cancer Res, 2018, doi:10.1158/0008-5472.CAN-18-0520. Nocodazole purchased from Selleck.

  • (B) HeLa cells were treated with DMSO, Taxol or Nocodazole (Noco). Total cell lysates were probed with the indicated antibodies on Phos-tag or regular SDS-polyacrylamide gels.

    Cell Signal, 2016, 28(12):1826-1832. Nocodazole purchased from Selleck.

  • A and B, SUM159 cells were treated with either DMSO, 20 μm Taxol, or 1 μm nocodazole (Noc) for 1-2 h before fixation. Fixed cells were co-stained for IRS-2 (green) and tubulin (red).

    J Biol Chem, 2017, 292(19):7806-7816. Nocodazole purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.
Targets
Microtubules [2]
(Cell-free assay)
Abl [1]
(Cell-free assay)
Abl (E255K) [1]
(Cell-free assay)
Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
In vitro

Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. [1] At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. [2] Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. [3] Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. [4]Nocodazole doubles HDR efficiency to up to 30% in iPSCs. It improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing[6].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat LT12 cells M4CzSHBzd2yrZnXyZZRqd25iYYPzZZk> NWHZSFRwPDhiaB?= MVPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IILheEBNXDF{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUC9OkBvVQ>? NG\pN20yQTJ{MECxPC=>
human PANC1 cells NVLte4hnWHKxbHnm[ZJifGmxbjDhd5NigQ>? MofsOFghcA>? MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCDTlOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OTBibl2= NWjibFFuOjV6Mke1NlI>
human HeLa cells NV3qXG1DWHKxbHnm[ZJifGmxbjDhd5NigQ>? NXznRXJFPDhiaB?= M3yxdmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXMZUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTF3IH7N NHr4OY4zPTh{N{WyNi=>
human MDA-MB-231 cells NFrwS|ZRem:uaX\ldoF1cW:wIHHzd4F6 Mk\3OFghcA>? M{j0WGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwQVE4KG6P M2DIOFI2QDJ5NUKy
human A549 cells MVvQdo9tcW[ncnH0bY9vKGG|c3H5 MnfYOFghcA>? M3;QRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTJyIH7N NYPxOXFVOjV6Mke1NlI>
lymphoid leukemia L1210 cells MVfQdo9tcW[ncnH0bY9vKGG|c3H5 NGfwZmg1QCCq NYfPcmhJUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDjeYx1fXKnZDDsfY1xcG:rZDDs[ZVs\W2rYTDMNVIyOCClZXzsd{BlfXKrbnegOFghcHK|LDDJSFUxRTJ5IH7N MnHQO|E{OTR6Mx?=
human A549 cells  NUXqSm04S3m2b4TvfIlkyqCjc4PhfS=> MUGyOEBp M135RWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2zOk43KG6P M2OwelIxOTFyMUO3
L1210 murine leukemia cell NY\2VVJGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1nXeGlvKH[rdILvJIZweiCrdIOgbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGwyOjFyIH31dolv\SCuZYXr[Y1q[SClZXzsJIdzd3e2aDygTWM2OD1|ODDuUS=> Mk\wNVc{QDF2Nh?=
mouse P388 cells Mmi4VJJwdGmoZYLheIlwdiCjc4PhfS=> NG[wZoI1QCCq NULmOYgzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDQN|g5KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDYWHQh[XO|YYmsJGlEPTB;NECgcm0> M3rpZVE6OjJyMEG4
LT12 cells NGC1RXZRem:uaX\ldoF1cW:wIHHzd4F6 MXO0PEBp MlL2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDMWFEzKGOnbHzzJIJ6KFiWVDDhd5NigSCjZoTldkA1QCCqcoOsJGlEPTB;NECgcm0> MYWxO|E5OTF4NB?=
human U2OS cells NIPZeWlHfW6ldHnvckBie3OjeR?= MYq3NkBp M3Hi[mlv\HWldHnvckBw\iCvaYTveIlkKGG{cnXzeEBqdiCqdX3hckBWOk:VIHPlcIx{KGG|c3Xzd4VlKGG|IH3peI91cWNiaX7k[Zgh[W[2ZYKgO|IhcHK|IHL5JI1q[3Kxc3PvdIlkKGGwYXz5d4l{NCCHQ{WwQVUxKG6P M4\qRVI1QTByOEi3
human NCI-H460 cells M{nPXnBzd2yrZnXyZZRqd25iYYPzZZk> MWm0PEBp MmrVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCQQ1mtTFQ3OCClZXzsd{Bi\nSncjC0PEBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;Nkigcm0> M1XCS|IyPTZ|N{Ww
human BT549 cells NHPtS3FRem:uaX\ldoF1cW:wIHHzd4F6 NXTre3R1PDhiaB?= M4PhUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS1OFkh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTZ7IH7N NELicHUzOTV4M{e1NC=>
human 451LU cells MV\Qdo9tcW[ncnH0bY9vKGG|c3H5 MWO0PEBp NYKwUpdpSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjC0OVFNXSClZXzsd{Bi\nSncjC0PEBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;N{Kgcm0> NUG4dJhHOjF3NkO3OVA>
human SKOV3 cells NWPkZ5prWHKxbHnm[ZJifGmxbjDhd5NigQ>? MUW0PEBp M1XaUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1vPWlMh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRThyIH7N MX6yNVU3Ozd3MB?=
K562 cells MYjGeY5kfGmxbjDhd5NigQ>? MoL3SYZn\WO2aY\lJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:gZZJz\XO2IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5m\CCNNU[yJINmdGy|IHnuJGczN01icHjhd4UtKEWFNUC9PFIhdk1? NX[0V2xyOTJ6NUK3Olg>
human SKBR3 cells NIK1dnJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFnFUlQ1QCCq NWW5[JI1T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hW0uEUkOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigQ>? MWSyNlg2ODJzNB?=
human COLO205 cells NXPXUJFHWHKxbHnm[ZJifGmxbjDhd5NigQ>? M1T4VVQ5KGh? Mme2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCFT1zPNlA2KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTCsJGlEPTB;OEigcm0> MnOyNlE2PjN5NUC=
human KB/HeLa cells NYS3U2tGTnWwY4Tpc44h[XO|YYm= NYD2cFZYOjRiaB?= M3vCbWNmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGtDN0inTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgS|IwVSCyaHHz[UBi[2O3bYXsZZRqd25iYX\0[ZIhOjRiaILzJIJ6KE[DQ2OgZY5idHm|aYOsJGVEPTB;OUGgcm0> NXHFR4JyOTl{MkCwNVg>
human A549 cells M4jZbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn\wS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gRVU1QSClZXzsd{BjgSCVdXzmc5Jpd2SjbXnu[UBDKGG|c3H5MEBIUTVyPUCuNUDPxE1? M13JSlIyOTB4NEW4
human MCF7 cells M4TjeWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXTHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNR2Y4KGOnbHzzJIJ6KFO3bH\vdohw\GGvaX7lJGIh[XO|YYmsJGdKPTB;MD6xJO69VQ>? NUSwe2lZOjFzME[0OVg>
human RKO cells M1LFUXBzd2yrZnXyZZRqd25iYYPzZZk> NYDscHZkSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDST28h[2WubIOgZpkhYFSWIHHzd4F6NCCLQ{WwQVAvOTFizszN NXrMVFBxOTl{MkCwNVg>
human SW480 cells NF3iXolRem:uaX\ldoF1cW:wIHHzd4F6 Ml;aOFghcA>? M4LFZWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e0PFAh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTBwMUKg{txO MX2yNVU3Ozd3MB?=
human NCI-H460 cells M3HVXXBzd2yrZnXyZZRqd25iYYPzZZk> MnjUOFghcA>? NIX3R5NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF7DTU1JPDZyIHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUC9NE4yPSEQvF2= MnP0NlA2Ozd5NkW=
human SF268 cells M17vfWN6fG:2b4jpZ:Kh[XO|YYm= NYHLU2ZpPDhiaB?= MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTSlI3QCClZXzsd{Bi\nSncjC0PEBpenNiYomgXHRVKGG|c3H5MEBKSzVyPUCuN{DPxE1? M{fiblIyPzB3MkKz
human SK-N-SH cells MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlvYS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV2suVi2VSDDj[YxteyCkeTDTeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvPjZizszN MkTINlEyODZ2NUi=
human MIAPaCa2 cells NWjSSHNrWHKxbHnm[ZJifGmxbjDhd5NigQ>? MYK0PEBp MWTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2LQWDhR4EzKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEeLNUC9NE46PSEQvF2= NGfuUoczPTF|MUm1Oi=>
human HaCaT cells MWHDfZRwfG:6aXRCpIF{e2G7 NWS0OpNGPDhiaB?= MoG4R5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHHDZXQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwh\3Kxd4ToJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD16Lkmg{txO M2K2U|I3OjNzMEiw
human SGC7901 cells NFvyO5VHfW6ldHnvckBie3OjeR?= NV3KN4x5PDhiaB?= NGXTU2ZKdmS3Y4Tpc44hd2ZiZHnzeI9zfGmxboOgbY4hfHWkdXzpckBie3OnbXLsfUBqdiCqdX3hckBUT0N5OUCxJINmdGy|IHHzd4V{e2WmIHHzJIFjdm:{bXHsJI1qfG:2aXOgd5BqdmSuZTDmc5Ju[XSrb36gZZQhOiCob3zkJGlEPTBibHX2[YwhcW6ldXLheIVlKG[xcjC0PEBpenNiYomgbY1ufW6xZnz1c5Jme2OnbnPlJJN1[WmwaX7nJIJie2WmIH\seY9z\XOlZX7j[UBucWO{b4Pjc5B6 MnnONlUzPjJyNUG=
human Jurkat T cells NVjWRY9wTnWwY4Tpc44h[XO|YYm= M2rlNlM3KGh? M2r4XWlv\HWldHnvckBw\iCvaXPyc5R2[nWuZTDkZY1i\2ViaX6gbJVu[W5iSoXyb4F1KFRiY3XscJMhd3[ncnX4dJJme3OrbnegRoNtOiCjZoTldkA{PiCqcoOgZpkhcW2vdX7vd5RicW6rbnegcYV1cG:m NWH2ZXhkOjJzOUezPVM>
human PC3M cells NWDIeXNbTnWwY4Tpc44h[XO|YYm= MVKwMlUhfWdxbXy= Mk\FNlQhcA>? M2DLeGlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iUFOzUUBk\WyuczDhd5Nme3OnZDDhd{Bi[2O3bYXsZZRqd25iYYSgd5VjTzFicHjhd4Uh[XRiMD61JJVoN22uIHHmeIVzKDJ2IHjyd{BjgSCobH;3JIN6fG:vZYTyfS=> NWf0VYJwOTh{NEe1O|M>
mouse NIH3T3 cells M2rRO2Z2dmO2aX;uJIF{e2G7 NEXldFQxNjVidXevcYw> MUGyOEBp Ml:0TY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDtc5V{\SCQSVizWFMh[2WubIOgZZN{\XO|ZXSgZZMh[WOldX31cIF1cW:wIHH0JJN2[kdzIIDoZZNmKGG2IECuOUB2\y:vbDDh[pRmeiB{NDDodpMh[nliZnzve{BkgXSxbXX0dpk> MX6xPFI1PzV5Mx?=

... Click to View More Cell Line Experimental Data

In vivo The tumor volume and tumor weight of the mice treated with Ketoconazole  plus  Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole  plus  Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole  or  Nocodazole alone. [5]

Protocol

Animal Research:

[5]

+ Expand
  • Animal Models: Nude mice with COLO-205 tumor xenografts
  • Formulation: 0.05% dimethyls
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 7 mg/mL (23.23 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 301.32
Formula

C14H11N3O3S

CAS No. 31430-18-9
Storage powder
in solvent
Synonyms Oncodazole

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Microtubule Associated Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID