RSL3

Catalog No.S8155 Synonyms: (1S,3R)-RSL3

For research use only.

RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.

RSL3 Chemical Structure

CAS No. 1219810-16-8

Selleck's RSL3 has been cited by 169 publications

Purity & Quality Control

Choose Selective Ferroptosis Inhibitors

Biological Activity

Description RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.
Targets
Gpx4 [1]
(In Calu-1 cells)
In vitro

Ferroptosis-inducing compounds inactivate GPX4 by direct binding or by depleting glutathione.After binding to GPX4, RSL3 inactivates GPX4 to induce ROS production from lipid peroxidation. RSL3's ability to induce synthetic lethality with oncogenic RAS is rapid and quite potent. This compound inhibits the growth of BJ-TERT/LT/ST/RASV12 and DRD cells as low as 10 ng/mland started to kill sensitive cells as early as 8 hr after treatment[1][2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MEFs and HT1080 cells MWTGeY5kfGmxbjDhd5NigQ>? M2fO[lAvPSEQvF2= M3\YbVI1KGh? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODZ6NkWzOEc,OzB4OE[1N|Q9N2F-
Jurkat M3jQT2NmdGxiZHXheIgh[XO|YYm= MWiwMlEh|ryP M3raWFI1KGhib4KgOFghcA>? MUnCWlYh[2:xcHXyZZRmeyC5aYToJHJUVDNidH:gbY5lfWOnIHPlcIwh\GWjdHisJIFk[2:vcHHubYVlKGK7IGLPV{Bxem:mdXP0bY9vKGGwZDDsbZBq\CCyZYLvfIll[XSrb36= MoTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3OEi0O|MoRjJ5NUi4OFc{RC:jPh?=
Molt-4 MX3D[YxtKGSnYYToJIF{e2G7 NV7yXGx3OC5yN{Wg{txO MXmyOEBpKG:{IES4JIg> MYrCWlYh[2:xcHXyZZRmeyC5aYToJHJUVDNidH:gbY5lfWOnIHPlcIwh\GWjdHisJIFk[2:vcHHubYVlKGK7IGLPV{Bxem:mdXP0bY9vKGGwZDDsbZBq\CCyZYLvfIll[XSrb36= M{PBflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUi4OFc{Lz5{N{W4PFQ4OzxxYU6=
RMS13 cells NE[3N3pE\WyuIHTlZZRpKGG|c3H5 M{HvV|AtKDZyLDCxNFAtKDF2MDDhcoQhOThyIN88US=> Mn\QOFghcA>? NH3N[5pC\GSrdHnvckBw\iCIZYLyc5N1[XSrbj2xJJNq\26rZnnjZY51dHlicnXkeYNm\CCUU1yzMUBweiCHcnHzeIlvNWmwZIXj[YQhdG:|czDv[kBk\WyuII\pZYJqdGm2eT6= NYr2VndIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xOVc4ODRpPkK2NVU4PzB2PD;hQi=>
BJeLR MVHDfZRwfG:6aXPpeJkh[XO|YYm= NWS0NWt3OC53NzD1US=> NEnSU4UyOiCq MVrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCToVNWiClZXzsd{BmgHC{ZYPzbY5oKFKDUzDHNVJXKG23dHHueEBifCByLkW3JJVOKGG2IEGyJIhzeyCkeTD0dplx[W5iYnz1[UB{fGGrbnnu[y=> NYHySGRURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|I{OjFpPkKyPFMzOzJzPD;hQi=>
BJeLR NHPKR4hEgXSxdH;4bYNqfHliYYPzZZk> NV:ySnBIOC53NzD1US=> NIX1ZlkzPCCq MnHCR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmpmVFJiY3XscJMh\XiycnXzd4lv\yCUQWOgS|EzXiCvdYThcpQh[XRiMD61O{B2VSCjdDCyOEBpenNiYomgeJJ6eGGwIHLseYUhe3SjaX7pcoc> MoT5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{KzNlEoRjJ{OEOyN|IyRC:jPh?=
BJeH NEnSdXpHfW6ldHnvckBie3OjeR?= M{TwelYhcA>? NYezXnl7UW6mdXP0bY9vKG:oIILlZYN1cX[nIH;4fYdmdiC|cHXjbYV{KHC{b3T1Z5Rqd25iaX6gbJVu[W5iQlrlTEBk\WyuczDlfJBz\XO|aX7nJJdqdGRidInw[UBTSVNiYX\0[ZIhPiCqcoOgZpkhTEOILXLhd4VlKG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=> MmfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{KzNlEoRjJ{OEOyN|IyRC:jPh?=
BJeLR NYn5U2x1TnWwY4Tpc44h[XO|YYm= NYPHTGpNPiCq M3TqNGlv\HWldHnvckBw\iC{ZXHjeIl3\SCxeInn[Y4he3CnY3nld{Bxem:mdXP0bY9vKGmwIHj1cYFvKEKMZVzSJINmdGy|IHX4dJJme3OrbnegVmFUKEdzMm[gcZV1[W62IHHmeIVzKDZiaILzJIJ6KESFRj3iZZNm\CCobH;3JIN6fG:vZYTybYMh[W6jbInzbZM> MoL3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{KzNlEoRjJ{OEOyN|IyRC:jPh?=
Assay
Methods Test Index PMID
Western blot GPX4 / Tranferrin / Ferritin ; HO-1 30524291 28515173
Growth inhibition assay Cell viability 26157704
Immunofluorescence ALOX12 / ALOX15 ; 4-HNE 28837253
In vivo Prostaglandin-endoperoxide synthase (PTGS) is the key enzyme in prostaglandin biosynthesis. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2. PTGS2 encoding cyclooxygenase-2 (COX-2) is significantly upregulated after treatment with RSL3 and erastin in mice[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: TERT/LT/ST/RASV12 cells 
  • Concentrations: 1 µg/ml
  • Incubation Time: 16hrs
  • Method:

    TERT/LT/ST/RASV12 cells are seeded in 10 cm dishes and treated with 1 µM staurosporine, 10 µg/ml erastin, 20 µg/ml RSL5, and 1 µg/ml RSL3 for 16 hr. Both dying cells and live cells in each 10 cm dish are harvested and collected in the same 15 ml tubes by centrifuging cell suspension at 1000 rpm for 5 min.

Animal Research:

[1]

  • Animal Models: athymic nude mice(8 weeks)implanted with subcutaneous xenograft tumors derived from BJeLR cells
  • Dosages: 100 mg/kg
  • Administration: s.c. injection

Solubility (25°C)

In vitro

DMSO 88 mg/mL
(199.6 mM)
Water Insoluble
Ethanol '''22 mg/mL

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.

30mg/mL

Chemical Information

Molecular Weight 440.88
Formula

C23H21ClN2O5

CAS No. 1219810-16-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC(=O)C1CC2=C(C(N1C(=O)CCl)C3=CC=C(C=C3)C(=O)OC)NC4=CC=CC=C24

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy RSL3 | RSL3 supplier | purchase RSL3 | RSL3 cost | RSL3 manufacturer | order RSL3 | RSL3 distributor