Catalog No.S8155 Synonyms: (1S,3R)-RSL3

For research use only.

RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.

RSL3 Chemical Structure

CAS No. 1219810-16-8

Selleck's RSL3 has been cited by 193 publications

Purity & Quality Control

Choose Selective Ferroptosis Inhibitors

Biological Activity

Description RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.
Gpx4 [1]
(In Calu-1 cells)
In vitro

Ferroptosis-inducing compounds inactivate GPX4 by direct binding or by depleting glutathione.After binding to GPX4, RSL3 inactivates GPX4 to induce ROS production from lipid peroxidation. RSL3's ability to induce synthetic lethality with oncogenic RAS is rapid and quite potent. This compound inhibits the growth of BJ-TERT/LT/ST/RASV12 and DRD cells as low as 10 ng/mland started to kill sensitive cells as early as 8 hr after treatment[1][2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MEFs and HT1080 cells M{DmdmZ2dmO2aX;uJIF{e2G7 M1zTS|AvPSEQvF2= MkSyNlQhcA>? Ml3pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB4OE[1N|QoRjNyNki2OVM1RC:jPh?=
Jurkat MmDNR4VtdCCmZXH0bEBie3OjeR?= M{njeFAvOSEQvF2= Mn23NlQhcCCxcjC0PEBp MljBRnY3KGOxb4DldoF1\XNid3n0bEBTW0x|IITvJIlv\HWlZTDj[YxtKGSnYYToMEBi[2OxbYDhcolm\CCkeTDSU3MheHKxZIXjeIlwdiCjbnSgcIlxcWRicHXyc5hq\GG2aX;u MnP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3OEi0O|MoRjJ5NUi4OFc{RC:jPh?=
Molt-4 M3;jc2NmdGxiZHXheIgh[XO|YYm= NYHCOWpPOC5yN{Wg{txO MoHmNlQhcCCxcjC0PEBp NHTIbIFDXjZiY3;vdIVz[XSnczD3bZRpKFKVTEOgeI8hcW6mdXPlJINmdGxiZHXheIgtKGGlY3;tdIFvcWWmIHL5JHJQWyCycn;keYN1cW:wIHHu[EBtcXCrZDDw[ZJwgGmmYYTpc44> M{XUe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUi4OFc{Lz5{N{W4PFQ4OzxxYU6=
RMS13 cells NF\l[WFE\WyuIHTlZZRpKGG|c3H5 MoDENEwhPjBuIEGwNEwhOTRyIHHu[EAyQDBizszN NGrYeIY1QCCq M3f1bGFl\Gm2aX;uJI9nKE[ncoLvd5RifGmwLUGgd4lodmmoaXPhcpRtgSC{ZXT1Z4VlKFKVTEOtJI9zKEW{YYP0bY4ucW6mdXPl[EBtd3O|IH;mJINmdGxidnnhZoltcXS7Lh?= NWT3SYd3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xOVc4ODRpPkK2NVU4PzB2PD;hQi=>
BJeLR M2\IOGN6fG:2b4jpZ4l1gSCjc4PhfS=> Mli2NE42PyC3TR?= M1f2RVEzKGh? NETTU25EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDUmWOUjDj[YxteyCneIDy[ZN{cW6pIGLBV{BIOTKYIH31eIFvfCCjdDCwMlU4KHWPIHH0JFEzKGi{czDifUB1enmyYX6gZox2\SC|dHHpcolv\w>? NGDYW2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizNlMzOSd-MkK4N|I{OjF:L3G+
BJeLR M2X5bGN6fG:2b4jpZ4l1gSCjc4PhfS=> NFSyUFQxNjV5IIXN NWfOZ|l7OjRiaB?= M2LiUmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJL\UyUIHPlcIx{KGW6cILld5NqdmdiUlHTJGcyOlZibYX0ZY51KGG2IECuOVchfU1iYYSgNlQhcHK|IHL5JJRzgXCjbjDicJVmKHO2YXnubY5o MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh|MkOyNUc,OjJ6M{KzNlE9N2F-
BJeH NWPQPVNQTnWwY4Tpc44h[XO|YYm= MWi2JIg> NUjOR|NqUW6mdXP0bY9vKG:oIILlZYN1cX[nIH;4fYdmdiC|cHXjbYV{KHC{b3T1Z5Rqd25iaX6gbJVu[W5iQlrlTEBk\WyuczDlfJBz\XO|aX7nJJdqdGRidInw[UBTSVNiYX\0[ZIhPiCqcoOgZpkhTEOILXLhd4VlKG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=> M1zSdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEOyN|IyLz5{MkizNlMzOTxxYU6=
BJeLR MXHGeY5kfGmxbjDhd5NigQ>? MVS2JIg> M1TIT2lv\HWldHnvckBw\iC{ZXHjeIl3\SCxeInn[Y4he3CnY3nld{Bxem:mdXP0bY9vKGmwIHj1cYFvKEKMZVzSJINmdGy|IHX4dJJme3OrbnegVmFUKEdzMm[gcZV1[W62IHHmeIVzKDZiaILzJIJ6KESFRj3iZZNm\CCobH;3JIN6fG:vZYTybYMh[W6jbInzbZM> NXmxdI94RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|I{OjFpPkKyPFMzOzJzPD;hQi=>
Methods Test Index PMID
Western blot GPX4 / Tranferrin / Ferritin ; HO-1 30524291 28515173
Growth inhibition assay Cell viability 26157704
Immunofluorescence ALOX12 / ALOX15 ; 4-HNE 28837253
In vivo Prostaglandin-endoperoxide synthase (PTGS) is the key enzyme in prostaglandin biosynthesis. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2. PTGS2 encoding cyclooxygenase-2 (COX-2) is significantly upregulated after treatment with RSL3 and erastin in mice[1].

Protocol (from reference)

Cell Research:


  • Cell lines: TERT/LT/ST/RASV12 cells 
  • Concentrations: 1 µg/ml
  • Incubation Time: 16hrs
  • Method:

    TERT/LT/ST/RASV12 cells are seeded in 10 cm dishes and treated with 1 µM staurosporine, 10 µg/ml erastin, 20 µg/ml RSL5, and 1 µg/ml RSL3 for 16 hr. Both dying cells and live cells in each 10 cm dish are harvested and collected in the same 15 ml tubes by centrifuging cell suspension at 1000 rpm for 5 min.

Animal Research:


  • Animal Models: athymic nude mice(8 weeks)implanted with subcutaneous xenograft tumors derived from BJeLR cells
  • Dosages: 100 mg/kg
  • Administration: s.c. injection

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.


Chemical Information

Molecular Weight 440.88


CAS No. 1219810-16-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC(=O)C1CC2=C(C(N1C(=O)CCl)C3=CC=C(C=C3)C(=O)OC)NC4=CC=CC=C24

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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