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Liproxstatin-1

Name: Liproxstatin-1
Brand: Selleck Chemicals
SKU: S7699
Rating: 5 (249 reviews)

Catalog No.S7699 Purity:99.88%

Liproxstatin-1 is a potent ferroptosis inhibitor with an IC50 of 22 nM.

Liproxstatin-1 Chemical Structure

CAS No. 950455-15-9

Purity & Quality Control

Batch: Purity: 99.88%

99.88

Products often used together with Liproxstatin-1

RSL3

Liproxstatin-1 and RSL3, in combination with iFSP1, induce cell death in human esophageal squamous cell carcinoma (ESCC) cell lines KYSE30/KYSE510/KYSE520.

Miyauchi W, et al. Esophagus. 2023 Jul;20(3):492-501.

DHA (Dihydroartemisinin)

Liproxstatin-1 and Dihydroartemisinin (DHA) have an antagonistic effect on the growth of blood-stage parasites of malaria.

Li S, et al. Biomed Pharmacother. 2022 Apr;148:112742.

iFSP1

Liproxstatin-1 and RSL3 combination induce cell death in human esophageal squamous cell carcinoma (ESCC) cell lines KYSE30/KYSE510/KYSE520.

Miyauchi W, et al. Esophagus. 2023 Jul;20(3):492-501.

S63845

Liproxstatin-1 completely protects BAX/BAK double knock-out (DKO) U251 cells from cell death induced by the combination of S63845 and A1331852.

Moujalled D, et al. Cell Death Differ. 2022 Jul;29(7):1335-1348.

A-1331852

Liproxstatin-1 effectively reduces cell death of U251 cells when treated with A1331852 plus S63845.

Moujalled D, et al. Cell Death Differ. 2022 Jul;29(7):1335-1348.

Liproxstatin-1 Related Products

Related Products	Fer-1 (Ferrostatin-1) Erastin RSL3 FIN56 IKE(Imidazole ketone erastin) UAMC-3203 iFSP1 SRS16-86 SRS11-92	Click to Expand
Related Compound Libraries	Metabolism Compound Library Anti-cancer Metabolism Compound Library Glutamine Metabolism Compound Library Carbohydrate Metabolism Compound Library Lipid Metabolism Compound Library	Click to Expand

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
MRC-5 lung fibroblast cells					DNDI: Cytotoxicity against human MRC-5 lung fibroblast cells, IC50 = 9.14 μM	ChEMBL
Click to View More Cell Line Experimental Data

Biological Activity

Description

Liproxstatin-1 is a potent ferroptosis inhibitor with an IC50 of 22 nM.

Targets

ferroptosis ^[1]
(Cell-free assay)

22 nM

In vitro
In vitro	Liproxstatin-1 is able to inhibit ferroptosis in the low nanomolar range and inhibit the growth of Gpx4^−/−cells with IC50 of 22 nM. Liproxstatin-1 (50 nM) completely prevents lipid peroxidation in Gpx4^−/−cells. Liproxstatin-1 (200 nM) protects against FINs, such as BSO (10 µM), erastin (1 µM) and RSL3 (0.5 µM), in a dose dependent manner, whereas it fails to rescue cell death induced by staurosporine (0.2 µM) and H₂O₂ (200 µM).^[1]
Cell Research	Cell lines	Gpx4^−/−cells
	Concentrations	~200 nM
	Incubation Time	~72 h
	Method	Cell viability is assessed at different time points after treatment using AquaBluer according to the manufacturer’s recommendations. Alternatively, cell death is also quantified by measuring released lactate dehydrogenase activity using the cytotoxicity detection kit (LDH).
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	COX2 / GPx4 / GAPDH β-actin / COX2 / GPx4 β-actin / COX2 / GPx4 NeuN / Syn / SNAP / GFAP / Actin / Tubulin GRP78 / CHOP / Actin GRP78 / CHOP / Actin GPX4 / β-actin		33889074
	Growth inhibition assay	Cell viability		31641008
	Immunofluorescence	β-actin / COX2 / GPx4 NeuN / ACSL4 mitochondrial Fe2+		30737476

In Vivo
In vivo	Liproxstatin-1 remarkably extends survival compared with the vehicle-treated group, delays ferroptosis in tubular cells, and mitigates tissue injury in ischaemia/reperfusion-induced liver injury. ^[1]
Animal Research	Animal Models	GreER^T2; Gpx4^fI/fI mice
	Dosages	10 mg/kg
	Administration	i.p.

References

[1] Friedmann Angeli JP, et al. Nat Cell Biol. 2014, 16(12):1180-1191.

Chemical Information & Solubility

Molecular Weight	340.85	Formula	C₁₉H₂₁ClN₄
CAS No.	950455-15-9	SDF	Download Liproxstatin-1 SDF
Smiles	C1CNCCC12C(=NCC3=CC(=CC=C3)Cl)NC4=CC=CC=C4N2
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 68 mg/mL ( (199.5 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 18 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	2%DMSO 1 40%PEG300 2 2%Tween80 3 56%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.000mg/ml (8.80mM)	Taking the 1 mL working solution as an example, add 20 μL of 150 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 20 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 560 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.000mg/ml (2.93mM)	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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