Liproxstatin-1

Catalog No.S7699

For research use only.

Liproxstatin-1 is a potent ferroptosis inhibitor with an IC50 of 22 nM.

Liproxstatin-1 Chemical Structure

CAS No. 950455-15-9

Selleck's Liproxstatin-1 has been cited by 101 publications

Purity & Quality Control

Choose Selective Ferroptosis Inhibitors

Biological Activity

Description Liproxstatin-1 is a potent ferroptosis inhibitor with an IC50 of 22 nM.
Targets
ferroptosis [1]
(Cell-free assay)
22 nM
In vitro

Liproxstatin-1 is able to inhibit ferroptosis in the low nanomolar range and inhibit the growth of Gpx4−/−cells with IC50 of 22 nM. Liproxstatin-1 (50 nM) completely prevents lipid peroxidation in Gpx4−/−cells. Liproxstatin-1 (200 nM) protects against FINs, such as BSO (10 µM), erastin (1 µM) and RSL3 (0.5 µM), in a dose dependent manner, whereas it fails to rescue cell death induced by staurosporine (0.2 µM) and H2O2 (200 µM).[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MRC-5 lung fibroblast cells MlfRSG5FUTpiQ4n0c5RwgGmlaYT5JIFo[Wmwc4SgbJVu[W5iTWLDMVUhdHWwZzDmbYJzd2KuYYP0JINmdGy|LDDJR|UxKD1iOT6xOEDPxE1? NULSbpJtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjB7NEe1NE8oRkOqRV3CUFww[T5?
Assay
Methods Test Index PMID
Western blot GPX4 / β-actin ; GRP78 / CHOP / Actin ; GRP78 / CHOP / Actin ; NeuN / Syn / SNAP / GFAP / Actin / Tubulin ; β-actin / COX2 / GPx4 ; β-actin / COX2 / GPx4 ; COX2 / GPx4 / GAPDH 33602915 29383150 28212525 30737476 33889074
Growth inhibition assay Cell viability 31641008
Immunofluorescence mitochondrial Fe2+ ; NeuN / ACSL4 ; β-actin / COX2 / GPx4 33343803 33314758 30737476
In vivo Liproxstatin-1 remarkably extends survival compared with the vehicle-treated group, delays ferroptosis in tubular cells, and mitigates tissue injury in ischaemia/reperfusion-induced liver injury. [1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Gpx4−/−cells
  • Concentrations: ~200 nM
  • Incubation Time: ~72 h
  • Method:

    Cell viability is assessed at different time points after treatment using AquaBluer according to the manufacturer’s recommendations. Alternatively, cell death is also quantified by measuring released lactate dehydrogenase activity using the cytotoxicity detection kit (LDH).

Animal Research:

[1]

  • Animal Models: GreERT2; Gpx4fI/fI mice
  • Dosages: 10 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

DMSO 68 mg/mL
(199.5 mM)
Water Insoluble
Ethanol '21 mg/mL warmed

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+40% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.

3mg/mL

Chemical Information

Molecular Weight 340.85
Formula

C19H21ClN4

CAS No. 950455-15-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CNCCC12C(=NCC3=CC(=CC=C3)Cl)NC4=CC=CC=C4N2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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