For research use only. Not for use in humans.

Catalog No.S7242

57 publications

Erastin Chemical Structure

Molecular Weight(MW): 547.04

Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.

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Selleck's Erastin has been cited by 57 publications

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Biological Activity

Description Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.
Ferroptosis [1]
In vitro

Erastin is selectively lethal to oncogenic RAS-mutant cell lines, and triggers a unique iron-dependent form of non-apoptotic cell death called ferroptosis. [1] [2] Erastin binds directly to VDAC2 and causes mitochondrial damage via ROS production in an NADH-dependent manner, which induces cell death in some tumor cells harbouring activating mutations in the RAS-RAF-MEK pathway. [3] In addition, erastin, via inducing ROS-mediated CID (Caspase-independent cell death), strongly enhances the effect of cisplatin in WT EGFR cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human CCF-STTG1 cells NX:3PWJqTnWwY4Tpc44h[XO|YYm= MnX4TY5pcWKrdHnvckBw\iC[Y4SgbY4hcHWvYX6gR2NHNVOWVFexJINmdGy|IHHzd4V{e2WmIHHzJIdtfXSjbXH0[UBz\WynYYPlJIFnfGW{IEKgbJJ{KGK7IH\seY9zd22ndIL5MEBKSzVyPUCuNkDPxE1w MlqwNlYzOzFzNU[=
human HeLa cells MmG2SpVv[3Srb36gZZN{[Xl? MoLGTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iSHXMZUBk\WyuczygSWM2OD1yLk[g{txONg>? M3LnNFE4PTZ6N{S4
human BJ cells M{Xhc2Z2dmO2aX;uJIF{e2G7 M4LkfWlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFLKJINmdGy|IHX4dJJme3OrbnegWGVTXCxiTGSsJHNVKGGwZDDSRXMhTzF{VjDteZRidnRiZ3Xu[ZMh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiUFStPVgxPTliYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKG2ndHjv[EwhUUN3ME2wMlkh|ryPLh?= MXqxO|U3QDd2OB?=
human HT1080 cells M4DhVmZ2dmO2aX;uJIF{e2G7 NF;FdHBKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDIWFExQDBiY3XscJMhcW5icILld4Vv[2Vib3[gVGQuQThyNUmgZpkhfHK7cHHuJIJtfWViZYjjcJV{cW:wIH3leIhw\CxiSVO1NF0yKM7:TT6= MnjzNVc2Pjh5NEi=
human SVR cells MU\GeY5kfGmxbjDhd5NigQ>? M1\QcGlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIGPWVkBk\WyuczygSWM2OD1{LkWg{txONg>? MkfWNVc2Pjh5NEi=
human MES-SA cells NU\NVphTTnWwY4Tpc44h[XO|YYm= M1nWUWlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIF3FV{1USSClZXzsd{whTUN3ME2zJO69VS5? NEDpcpEyPzV4OEe0PC=>
human SKUT cells MWPGeY5kfGmxbjDhd5NigQ>? MXPJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCVS2XUJINmdGy|LDDFR|UxRTRizszNMi=> NF2zNWIyPzV4OEe0PC=>
human Calu1 cells NInlZ2ZHfW6ldHnvckBie3OjeR?= MlrVTY5pcWKrdHnvckBw\iCqdX3hckBE[Wy3MTDj[YxteyCneIDy[ZN{cW6pIFvSRXMhf2m2aDDhZ5RqfmG2aX7nJI12fGG2aX;ud{BjgSC2conwZY4h[my3ZTDlfINtfXOrb36gZZN{[XluIFnDOVA:PCEQvF2u M{T3VVE4PTZ6N{S4
human LNCaP cells M2L4XmZ2dmO2aX;uJIF{e2G7 MYfJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCOTlPhVEBk\WyuczygSWM2OD14IN88UU4> MkHNNVc2Pjh5NEi=
human U2OS cells NIX1eFZHfW6ldHnvckBie3OjeR?= NX2wUIJxUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gWVJQWyClZXzsd{whTUN3ME22JO69VS5? MWWxO|U3QDd2OB?=
human TC32 cells M2rsPWZ2dmO2aX;uJIF{e2G7 NYDBeJg{UW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gWGM{OiClZXzsdy=> NF7BW3AyPzV4OEe0PC=>
human SK-N-MC cells MX3GeY5kfGmxbjDhd5NigQ>? MnziTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iU1utUk1OSyClZXzsd{whTUN3ME2xNEDPxE1w MUGxO|U3QDd2OB?=
human U937 cells MkfNSpVv[3Srb36gZZN{[Xl? NH\KUmlKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDVPVM4KGOnbHzzMEBGSzVyPUGwJO69VS5? MXexO|U3QDd2OB?=
human TC71 cells MUnGeY5kfGmxbjDhd5NigQ>? NX7EUZJCUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gWGM4OSClZXzsd{whTUN3ME2xNEDPxE1w NV;qUHpYOTd3Nki3OFg>
human BJ cells NYXtVpBQTnWwY4Tpc44h[XO|YYm= NGi1XppKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBVTVKWIHX4dJJme3OrbnegbJVu[W5iQlqgZ4VtdHNuIFXDOVA:OTBizszNMi=> M2Pac|E4PTZ6N{S4
human EWS502 cells NXvVdXU3TnWwY4Tpc44h[XO|YYm= MXjJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCHV2O1NFIh[2WubIOsJGVEPTB;MUCg{txONg>? NF;odXMyPzV4OEe0PC=>
human Hs51.T cells MnXjSpVv[3Srb36gZZN{[Xl? MnLQTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iSIO1NU5VKGOnbHzzMEBGSzVyPUGyJO69VS5? MoL0NVc2Pjh5NEi=
human Hs925.T cells MmLsSpVv[3Srb36gZZN{[Xl? MYjJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCKc{myOU5VKGOnbHzzMEBGSzVyPUG3JO69VS5? NUf2fGprOTd3Nki3OFg>
human HOS cells NUixWJhiTnWwY4Tpc44h[XO|YYm= MmrlTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iSF;TJINmdGy|IDygSWM2OD1zNzFOwG0v MmTtNVc2Pjh5NEi=
human MX2 cells NVz1[HJoTnWwY4Tpc44h[XO|YYm= NWexSotnUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gUXgzKGOnbHzzMEBGSzVyPUG4JO69VS5? MoraNVc2Pjh5NEi=
human A673 cells Mlr4SpVv[3Srb36gZZN{[Xl? Ml22TY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iQU[3N{Bk\WyuczygSWM2OD1|MDFOwG0v NVHZPXd1OTd3Nki3OFg>
human BJ cells MUjGeY5kfGmxbjDhd5NigQ>? MULJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCESjDj[YxteyCneIDy[ZN{cW6pIGTFVnQtKEyWLDDTWEBidmRiUlHTJGcyOlZibYX0ZY51KGenbnXzJIlvKHC{ZYPlcoNmKG:oIGWwNVI3KGK7IITyfZBidiCkbIXlJIV5[2y3c3nvckBu\XSqb3SsJGlEPTB;M{GuNkDPxE1w MXyxO|U3QDd2OB?=
human BJ cells M4n2cWZ2dmO2aX;uJIF{e2G7 MVu5JO69VQ>? MXHJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCESjDj[YxteyCneIDy[ZN{cW6pIGTFVnQtKEyWLDDTWEBidmRiUlHTJGcyOlZibYX0ZY51KGenbnXzJINmdGy|IHH0JFkhfU1w NUjtXYpROTd3Nki3OFg>
human BJ cells M1HT[mZ2dmO2aX;uJIF{e2G7 MWe0MlYh|ryP NH;NR4FKdmO{ZXHz[UBqdiCrboTyZYNmdGy3bHHyJI95cWSjdHn2[UB{eGWlaXXzJIlvKGi3bXHuJGJLKGOnbHzzJIV5eHKnc4PpcochXEWUVDygUHQtKFOWIHHu[EBTSVNiR{GyWkBufXSjboSg[4Vv\XNiY3XscJMh[XRiND62JJVO M4TEPVE4PTZ6N{S4
human BJeLR cells MYPDfZRwfG:6aXRCpIF{e2G7 Ml\uNVAh|ryP M3vLfFEzKGh? MkfrR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmpmVFJiY3XscJMh\XiycnXzd4lv\yCUQWOgS|EzXiCvdYThcpQh[XRiMUCgeW0h[XRiMUKgbJJ{KGK7IITyfZBidiCkbIXlJJN1[WmwaX7n NW\WS2ZJOjJ6M{KzNlE>
human BJeH cells NVrqPYNITnWwY4Tpc44h[XO|YYm= MUG2JIg> MkPZTY5lfWO2aX;uJI9nKHKnYXP0bZZmKG:6eXflckB{eGWlaXXzJJBzd2S3Y4Tpc44hcW5iaIXtZY4hSkqnSDDj[YxteyCneIDy[ZN{cW6pIIfpcIQhfHmyZTDSRXMh[W[2ZYKgOkBpenNiYomgSGNHNWKjc3XkJIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?= Mnf0NlI5OzJ|MkG=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
GPX4 / cleaved-PARP / cleaved-caspase3 / LC3 / p62 / LDH / HMGB1; 

PubMed: 27308510     

HL-60 and Jurkat cells were treated with erastin (5 μM) for 24 h and subjected to western blot analysis of the indicated proteins in whole cell extracts or supernatant. 

TfR1 / p-JNK / JNK / p-P38; 

PubMed: 31105999     

HL-60/NRAS (Q61L) cells were treated with erastin (5 μM) combined with SP600125 (10 μM) and SB202190 (10 μM) for 48 h. TfR1 expression and the phosphorylation of p38 (p-P38) and JNK1/2 (p-JNK1/2) were assayed by western blot. 

HSPA5 / p-EIF2AK3; 

PubMed: 28130223     

Western blot analysis indicated protein expression in PDAC cells following treatment with erastin (2.5-40 μM) for 24 hours (n=3, *p < 0.05 versus untreated group). 


PubMed: 29383150     

Cells were treated with 50 μM erastin for various time(1-24 h). Whole-cell extracts were analyzed with immunoblotting assay.

27308510 31105999 28130223 29383150
Growth inhibition assay
Cell survival; 

PubMed: 29348676     

The A375 and G-361 human melanoma cells were treated with erastin (2.5-40 µM) or RSL3 (0.1-10 µM) with or without a cell death inhibitor (ferrostatin-1, 1 µM; ZVAD-FMK, 10 µM; necrosulfonamide, 0.5 µM) for 24 h. Cell death was assayed using a CCK-8 kit. Data shown represent mean ± SD from three independent experiments. ****p < 0.0001.


PubMed: 31105999     

HL-60/NRAS (Q61L) cells were treated with erastin (5 μM) with or without Fer-1 (1 μM) pretreatment for 48 h, and then the nuclear/cytosolic HMGB1 expression was assayed by immunofluorescence(Green, HMGB1; blue, nucleus). 



Cell Research:[1]
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  • Cell lines: BJ-TERT/LT/ST/RASV12 cells
  • Concentrations: 5 or 10 μg/mL
  • Incubation Time: 6-11 hours
  • Method: BJ-TERT/LT/ST/RASV12 cells are seeded in 100 mm dishes and allowed to grow overnight. Cells are treated with erastin (5 or 10 μg/ml) for 6, 8, or 11 hr. A camptothecin-treated (0.4 μg/ml) control is maintained, treated at the time of seeding for 20 hours. After the treatment, cells are harvested with trypsin/EDTA and washed once with fresh medium containing serum and then twice with phosphate-buffered saline. Cells are resuspended in 1× binding buffer. 100 μL is incubated with 5 μL of Annexin V-FITC and propidium iodiode for 15 min in the dark at room temperature. Then 400 μl of the 1× binding buffer s added and the cells analyzed by flow cytometry. Data are acquired and analyzed using Cellquest software. Only viable cells that do not stain with propidium iodiode are analzyed for Annexin V-FITC staining using the FL1 channel.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 19 mg/mL (34.73 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 547.04


CAS No. 571203-78-6
Storage powder
in solvent
Synonyms N/A
Smiles CCOC1=C(C=CC=C1)N2C(=O)C3=C(C=CC=C3)N=C2C(C)N4CCN(CC4)C(=O)COC5=CC=C(Cl)C=C5

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID