For research use only.

Catalog No.S7242

75 publications

Erastin Chemical Structure

Molecular Weight(MW): 547.04

Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.

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Selleck's Erastin has been cited by 75 publications

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Biological Activity

Description Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.
Ferroptosis [1]
In vitro

Erastin is selectively lethal to oncogenic RAS-mutant cell lines, and triggers a unique iron-dependent form of non-apoptotic cell death called ferroptosis. [1] [2] Erastin binds directly to VDAC2 and causes mitochondrial damage via ROS production in an NADH-dependent manner, which induces cell death in some tumor cells harbouring activating mutations in the RAS-RAF-MEK pathway. [3] In addition, erastin, via inducing ROS-mediated CID (Caspase-independent cell death), strongly enhances the effect of cisplatin in WT EGFR cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human CCF-STTG1 cells M16wWGZ2dmO2aX;uJIF{e2G7 MlPsTY5pcWKrdHnvckBw\iC[Y4SgbY4hcHWvYX6gR2NHNVOWVFexJINmdGy|IHHzd4V{e2WmIHHzJIdtfXSjbXH0[UBz\WynYYPlJIFnfGW{IEKgbJJ{KGK7IH\seY9zd22ndIL5MEBKSzVyPUCuNkDPxE1w NHLBOHEzPjJ|MUG1Oi=>
human HeLa cells NGH4U|FHfW6ldHnvckBie3OjeR?= MX7JcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCKZVzhJINmdGy|LDDFR|UxRTBwNjFOwG0v NWDzfGRXOTd3Nki3OFg>
human BJ cells MYnGeY5kfGmxbjDhd5NigQ>? MVTJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCESjDj[YxteyCneIDy[ZN{cW6pIGTFVnQtKEyWLDDTWEBidmRiUlHTJGcyOlZibYX0ZY51KGenbnXzJINmdGy|IHnuJJBz\XOnbnPlJI9nKFCGLUm4NFU6KGK7IITyfZBidiCkbIXlJIV5[2y3c3nvckBu\XSqb3SsJGlEPTB;MD65JO69VS5? MWCxO|U3QDd2OB?=
human HT1080 cells NEDlVFJHfW6ldHnvckBie3OjeR?= M2n3dGlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFjUNVA5OCClZXzsd{BqdiCycnXz[Y5k\SCxZjDQSE06QDB3OTDifUB1enmyYX6gZox2\SCneHPseZNqd25ibXX0bI9lNCCLQ{WwQVEh|ryPLh?= MVKxO|U3QDd2OB?=
human SVR cells MXvGeY5kfGmxbjDhd5NigQ>? MnuxTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iU2\SJINmdGy|LDDFR|UxRTJwNTFOwG0v M2XlU|E4PTZ6N{S4
human MES-SA cells NVrKdm1NTnWwY4Tpc44h[XO|YYm= NWPXXZJkUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gUWVUNVODIHPlcIx{NCCHQ{WwQVMh|ryPLh?= M4H2UVE4PTZ6N{S4
human SKUT cells NHz0b3lHfW6ldHnvckBie3OjeR?= MX;JcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCVS2XUJINmdGy|LDDFR|UxRTRizszNMi=> M2XJXVE4PTZ6N{S4
human Calu1 cells M4T5cGZ2dmO2aX;uJIF{e2G7 MVrJcohq[mm2aX;uJI9nKGi3bXHuJGNidHVzIHPlcIx{KGW6cILld5NqdmdiS2LBV{B4cXSqIHHjeIl3[XSrbnegcZV1[XSrb37zJIJ6KHS{eYDhckBjdHWnIHX4Z4x2e2mxbjDhd5NigSxiSVO1NF01KM7:TT6= NYnOc|ZCOTd3Nki3OFg>
human LNCaP cells NW\UOJhwTnWwY4Tpc44h[XO|YYm= MoTKTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iTF7DZXAh[2WubIOsJGVEPTB;NjFOwG0v MnjtNVc2Pjh5NEi=
human U2OS cells NYfJ[4dNTnWwY4Tpc44h[XO|YYm= NUfpNoR2UW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gWVJQWyClZXzsd{whTUN3ME22JO69VS5? M2myPFE4PTZ6N{S4
human TC32 cells Mn\MSpVv[3Srb36gZZN{[Xl? NFnvfGJKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDUR|MzKGOnbHzz M4rVc|E4PTZ6N{S4
human SK-N-MC cells MnvsSpVv[3Srb36gZZN{[Xl? MXjJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCVSz3OMW1EKGOnbHzzMEBGSzVyPUGwJO69VS5? MUGxO|U3QDd2OB?=
human U937 cells NGXNTpNHfW6ldHnvckBie3OjeR?= NXztOZRPUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gWVk{PyClZXzsd{whTUN3ME2xNEDPxE1w NETNVpEyPzV4OEe0PC=>
human TC71 cells M{fobWZ2dmO2aX;uJIF{e2G7 NF\DfYpKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDUR|cyKGOnbHzzMEBGSzVyPUGwJO69VS5? MXqxO|U3QDd2OB?=
human BJ cells MUXGeY5kfGmxbjDhd5NigQ>? M4XVc2lv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJHRGWlRiZYjwdoV{e2mwZzDoeY1idiCESjDj[YxteyxiRVO1NF0yOCEQvF2u NXvFT4wyOTd3Nki3OFg>
human EWS502 cells MYTGeY5kfGmxbjDhd5NigQ>? MWnJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCHV2O1NFIh[2WubIOsJGVEPTB;MUCg{txONg>? MlHPNVc2Pjh5NEi=
human Hs51.T cells NWOy[G1jTnWwY4Tpc44h[XO|YYm= M{nURWlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFjzOVEvXCClZXzsd{whTUN3ME2xNkDPxE1w M3\meVE4PTZ6N{S4
human Hs925.T cells MnnuSpVv[3Srb36gZZN{[Xl? M2KwSmlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFjzPVI2NlRiY3XscJMtKEWFNUC9NVch|ryPLh?= NF\5OFYyPzV4OEe0PC=>
human HOS cells NVz6bIUzTnWwY4Tpc44h[XO|YYm= MYLJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCKT2OgZ4VtdHNiLDDFR|UxRTF5IN88UU4> NEHUbo8yPzV4OEe0PC=>
human MX2 cells NF\HNXNHfW6ldHnvckBie3OjeR?= MnvkTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iTWiyJINmdGy|LDDFR|UxRTF6IN88UU4> NWnY[XM{OTd3Nki3OFg>
human A673 cells M3SxNWZ2dmO2aX;uJIF{e2G7 MULJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCDNkezJINmdGy|LDDFR|UxRTNyIN88UU4> NF\hcmIyPzV4OEe0PC=>
human BJ cells NFLGWGhHfW6ldHnvckBie3OjeR?= MlrYTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iQlqgZ4VtdHNiZYjwdoV{e2mwZzDUSXJVNCCOVDygV3Qh[W6mIGLBV{BIOTKYIH31eIFvfCCpZX7ld{BqdiCycnXz[Y5k\SCxZjDVNFEzPiCkeTD0dplx[W5iYnz1[UBmgGOudYPpc44hdWW2aH;kMEBKSzVyPUOxMlIh|ryPLh?= MUGxO|U3QDd2OB?=
human BJ cells MkTtSpVv[3Srb36gZZN{[Xl? NWnHNmJqQSEQvF2= M2PMeWlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFLKJINmdGy|IHX4dJJme3OrbnegWGVTXCxiTGSsJHNVKGGwZDDSRXMhTzF{VjDteZRidnRiZ3Xu[ZMh[2WubIOgZZQhQSC3TT6= NF76U4oyPzV4OEe0PC=>
human BJ cells NUjG[|JJTnWwY4Tpc44h[XO|YYm= MWm0MlYh|ryP M3rFUmlv[3KnYYPlJIlvKGmwdILhZ4VtdHWuYYKgc5hq\GG2aY\lJJNx\WOrZYOgbY4hcHWvYX6gRmoh[2WubIOg[ZhxemW|c3nu[{BVTVKWLDDMWEwhW1RiYX7kJHJCWyCJMULWJI12fGGwdDDn[Y5meyClZXzsd{BifCB2Lk[geW0> NUfIe41XOTd3Nki3OFg>
human BJeLR cells NEfjV2VEgXSxdH;4bYPDqGG|c3H5 M1PyUlExKM7:TR?= MUixNkBp MX;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCToVNWiClZXzsd{BmgHC{ZYPzbY5oKFKDUzDHNVJXKG23dHHueEBifCBzMDD1UUBifCBzMjDodpMh[nlidIL5dIFvKGKudXWgd5RicW6rbne= M3HqN|IzQDN{M{Kx
human BJeH cells MnjvSpVv[3Srb36gZZN{[Xl? MoDZOkBp NYPXbnVKUW6mdXP0bY9vKG:oIILlZYN1cX[nIH;4fYdmdiC|cHXjbYV{KHC{b3T1Z5Rqd25iaX6gbJVu[W5iQlrlTEBk\WyuczDlfJBz\XO|aX7nJJdqdGRidInw[UBTSVNiYX\0[ZIhPiCqcoOgZpkhTEOILXLhd4VlKG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=> MUmyNlg{OjN{MR?=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
GPX4 / cleaved-PARP / cleaved-caspase3 / LC3 / p62 / LDH / HMGB1; 

PubMed: 27308510     

HL-60 and Jurkat cells were treated with erastin (5 μM) for 24 h and subjected to western blot analysis of the indicated proteins in whole cell extracts or supernatant. 

TfR1 / p-JNK / JNK / p-P38; 

PubMed: 31105999     

HL-60/NRAS (Q61L) cells were treated with erastin (5 μM) combined with SP600125 (10 μM) and SB202190 (10 μM) for 48 h. TfR1 expression and the phosphorylation of p38 (p-P38) and JNK1/2 (p-JNK1/2) were assayed by western blot. 

HSPA5 / p-EIF2AK3; 

PubMed: 28130223     

Western blot analysis indicated protein expression in PDAC cells following treatment with erastin (2.5-40 μM) for 24 hours (n=3, *p < 0.05 versus untreated group). 


PubMed: 29383150     

Cells were treated with 50 μM erastin for various time(1-24 h). Whole-cell extracts were analyzed with immunoblotting assay.

27308510 31105999 28130223 29383150
Growth inhibition assay
Cell survival; 

PubMed: 29348676     

The A375 and G-361 human melanoma cells were treated with erastin (2.5-40 µM) or RSL3 (0.1-10 µM) with or without a cell death inhibitor (ferrostatin-1, 1 µM; ZVAD-FMK, 10 µM; necrosulfonamide, 0.5 µM) for 24 h. Cell death was assayed using a CCK-8 kit. Data shown represent mean ± SD from three independent experiments. ****p < 0.0001.


PubMed: 31105999     

HL-60/NRAS (Q61L) cells were treated with erastin (5 μM) with or without Fer-1 (1 μM) pretreatment for 48 h, and then the nuclear/cytosolic HMGB1 expression was assayed by immunofluorescence(Green, HMGB1; blue, nucleus). 



Cell Research:[1]
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  • Cell lines: BJ-TERT/LT/ST/RASV12 cells
  • Concentrations: 5 or 10 μg/mL
  • Incubation Time: 6-11 hours
  • Method: BJ-TERT/LT/ST/RASV12 cells are seeded in 100 mm dishes and allowed to grow overnight. Cells are treated with erastin (5 or 10 μg/ml) for 6, 8, or 11 hr. A camptothecin-treated (0.4 μg/ml) control is maintained, treated at the time of seeding for 20 hours. After the treatment, cells are harvested with trypsin/EDTA and washed once with fresh medium containing serum and then twice with phosphate-buffered saline. Cells are resuspended in 1× binding buffer. 100 μL is incubated with 5 μL of Annexin V-FITC and propidium iodiode for 15 min in the dark at room temperature. Then 400 μl of the 1× binding buffer s added and the cells analyzed by flow cytometry. Data are acquired and analyzed using Cellquest software. Only viable cells that do not stain with propidium iodiode are analzyed for Annexin V-FITC staining using the FL1 channel.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 19 mg/mL (34.73 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG 300+5%Tween80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 547.04


CAS No. 571203-78-6
Storage 3 years -20°C powder
1 month -80°C in solvent
Synonyms N/A
Smiles CCOC1=C(C=CC=C1)N2C(=O)C3=C(C=CC=C3)N=C2C(C)N4CCN(CC4)C(=O)COC5=CC=C(Cl)C=C5

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID