Catalog No.S7242

Erastin Chemical Structure

Molecular Weight(MW): 547.04

Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.

Size Price Stock Quantity  
USD 147 In stock
USD 770 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 27 Publications

Purity & Quality Control

Choose Selective Ferroptosis Inhibitors

Biological Activity

Description Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.
Ferroptosis [1]
In vitro

Erastin is selectively lethal to oncogenic RAS-mutant cell lines, and triggers a unique iron-dependent form of non-apoptotic cell death called ferroptosis. [1] [2] Erastin binds directly to VDAC2 and causes mitochondrial damage via ROS production in an NADH-dependent manner, which induces cell death in some tumor cells harbouring activating mutations in the RAS-RAF-MEK pathway. [3] In addition, erastin, via inducing ROS-mediated CID (Caspase-independent cell death), strongly enhances the effect of cisplatin in WT EGFR cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HeLa cells M1PnOGZ2dmO2aX;uJIF{e2G7 M33sNmlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFjlUIEh[2WubIOsJGVEPTB;MD62JO69VS5? MmLONVc2Pjh5NEi=
human BJ cells NGf6THBHfW6ldHnvckBie3OjeR?= MX\JcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCESjDj[YxteyCneIDy[ZN{cW6pIGTFVnQtKEyWLDDTWEBidmRiUlHTJGcyOlZibYX0ZY51KGenbnXzJINmdGy|IHnuJJBz\XOnbnPlJI9nKFCGLUm4NFU6KGK7IITyfZBidiCkbIXlJIV5[2y3c3nvckBu\XSqb3SsJGlEPTB;MD65JO69VS5? NYLodmlUOTd3Nki3OFg>
human HT1080 cells NX3K[VB{TnWwY4Tpc44h[XO|YYm= NXrmTGVwUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gTHQyODhyIHPlcIx{KGmwIIDy[ZNmdmOnIH;mJHBFNTl6MEW5JIJ6KHS{eYDhckBjdHWnIHX4Z4x2e2mxbjDt[ZRpd2RuIFnDOVA:OSEQvF2u MUOxO|U3QDd2OB?=
human SVR cells NU\JZ5hVTnWwY4Tpc44h[XO|YYm= MXLJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCVVmKgZ4VtdHNuIFXDOVA:Oi53IN88UU4> M3r0[FE4PTZ6N{S4
human MES-SA cells M{XZTGZ2dmO2aX;uJIF{e2G7 M4HJ[mlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIF3FV{1USSClZXzsd{whTUN3ME2zJO69VS5? NF25SooyPzV4OEe0PC=>
human SKUT cells MWLGeY5kfGmxbjDhd5NigQ>? MmL1TY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iU1vVWEBk\WyuczygSWM2OD12IN88UU4> MoHONVc2Pjh5NEi=
human Calu1 cells MkXJSpVv[3Srb36gZZN{[Xl? MmLyTY5pcWKrdHnvckBw\iCqdX3hckBE[Wy3MTDj[YxteyCneIDy[ZN{cW6pIFvSRXMhf2m2aDDhZ5RqfmG2aX7nJI12fGG2aX;ud{BjgSC2conwZY4h[my3ZTDlfINtfXOrb36gZZN{[XluIFnDOVA:PCEQvF2u NInER4IyPzV4OEe0PC=>
human LNCaP cells Mn\vSpVv[3Srb36gZZN{[Xl? MWfJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCOTlPhVEBk\WyuczygSWM2OD14IN88UU4> MUexO|U3QDd2OB?=
human U2OS cells NE[3bWRHfW6ldHnvckBie3OjeR?= MnzTTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iVULPV{Bk\WyuczygSWM2OD14IN88UU4> M3TJbFE4PTZ6N{S4
human TC32 cells MUfGeY5kfGmxbjDhd5NigQ>? M4Twd2lv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIGTDN|Ih[2WubIO= NH;QPXoyPzV4OEe0PC=>
human SK-N-MC cells MlvtSpVv[3Srb36gZZN{[Xl? MUPJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCVSz3OMW1EKGOnbHzzMEBGSzVyPUGwJO69VS5? NHfyO4IyPzV4OEe0PC=>
human U937 cells NHzsZ4VHfW6ldHnvckBie3OjeR?= MUjJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCXOUO3JINmdGy|LDDFR|UxRTFyIN88UU4> M2rpfVE4PTZ6N{S4
human TC71 cells M2jLZ2Z2dmO2aX;uJIF{e2G7 NWLV[ox4UW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gWGM4OSClZXzsd{whTUN3ME2xNEDPxE1w MX[xO|U3QDd2OB?=
human BJ cells MlziSpVv[3Srb36gZZN{[Xl? MkXZTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gWGVTXCCneIDy[ZN{cW6pIHj1cYFvKEKMIHPlcIx{NCCHQ{WwQVExKM7:TT6= MX2xO|U3QDd2OB?=
human EWS502 cells NIT0cotHfW6ldHnvckBie3OjeR?= NIPyXJlKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDFW3M2ODJiY3XscJMtKEWFNUC9NVAh|ryPLh?= M2XzSlE4PTZ6N{S4
human Hs51.T cells NH7MVJVHfW6ldHnvckBie3OjeR?= NXvBSJZuUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gTJM2OS6WIHPlcIx{NCCHQ{WwQVEzKM7:TT6= MliyNVc2Pjh5NEi=
human Hs925.T cells MXXGeY5kfGmxbjDhd5NigQ>? NFT2dYFKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDId|kzPS6WIHPlcIx{NCCHQ{WwQVE4KM7:TT6= MXOxO|U3QDd2OB?=
human HOS cells MmTFSpVv[3Srb36gZZN{[Xl? MVfJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCKT2OgZ4VtdHNiLDDFR|UxRTF5IN88UU4> MUCxO|U3QDd2OB?=
human MX2 cells NV[zeVRLTnWwY4Tpc44h[XO|YYm= MVTJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCPWEKgZ4VtdHNuIFXDOVA:OThizszNMi=> MYixO|U3QDd2OB?=
human A673 cells M4PGOmZ2dmO2aX;uJIF{e2G7 NWXvS5JOUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gRVY4OyClZXzsd{whTUN3ME2zNEDPxE1w NGjJTnEyPzV4OEe0PC=>
human BJ cells M{DoUWZ2dmO2aX;uJIF{e2G7 MkjYTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iQlqgZ4VtdHNiZYjwdoV{e2mwZzDUSXJVNCCOVDygV3Qh[W6mIGLBV{BIOTKYIH31eIFvfCCpZX7ld{BqdiCycnXz[Y5k\SCxZjDVNFEzPiCkeTD0dplx[W5iYnz1[UBmgGOudYPpc44hdWW2aH;kMEBKSzVyPUOxMlIh|ryPLh?= NHrDV5kyPzV4OEe0PC=>
human BJ cells MoroSpVv[3Srb36gZZN{[Xl? MVe5JO69VQ>? M4rDOmlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFLKJINmdGy|IHX4dJJme3OrbnegWGVTXCxiTGSsJHNVKGGwZDDSRXMhTzF{VjDteZRidnRiZ3Xu[ZMh[2WubIOgZZQhQSC3TT6= MVGxO|U3QDd2OB?=
human BJ cells MWLGeY5kfGmxbjDhd5NigQ>? Mni0OE43KM7:TR?= NUnleGdpUW6lcnXhd4UhcW5iaX70doFk\WyudXzhdkBwgGmmYYTpeoUhe3CnY3nld{BqdiCqdX3hckBDUiClZXzsd{BmgHC{ZYPzbY5oKFSHUmSsJGxVNCCVVDDhcoQhWkGVIFexNnYhdXW2YX70JIdmdmW|IHPlcIx{KGG2IESuOkB2VQ>? M13SNVE4PTZ6N{S4
human BJeLR cells MkWzR5l1d3SxeHnjxsBie3OjeR?= M1jO[VExKM7:TR?= M1ru[|EzKGh? M2rkOGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJL\UyUIHPlcIx{KGW6cILld5NqdmdiUlHTJGcyOlZibYX0ZY51KGG2IEGwJJVOKGG2IEGyJIhzeyCkeTD0dplx[W5iYnz1[UB{fGGrbnnu[y=> NVrRdo9DOjJ6M{KzNlE>
human BJeH cells NU\BO5N6TnWwY4Tpc44h[XO|YYm= Mn3tOkBp MmXkTY5lfWO2aX;uJI9nKHKnYXP0bZZmKG:6eXflckB{eGWlaXXzJJBzd2S3Y4Tpc44hcW5iaIXtZY4hSkqnSDDj[YxteyCneIDy[ZN{cW6pIIfpcIQhfHmyZTDSRXMh[W[2ZYKgOkBpenNiYomgSGNHNWKjc3XkJIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?= NYPoU|Q{OjJ6M{KzNlE>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
GPX4 / cleaved-PARP / cleaved-caspase3 / LC3 / p62 / LDH / HMGB1; 

PubMed: 27308510     

HL-60 and Jurkat cells were treated with erastin (5 μM) for 24 h and subjected to western blot analysis of the indicated proteins in whole cell extracts or supernatant. 

TfR1 / p-JNK / JNK / p-P38; 

PubMed: 31105999     

HL-60/NRAS (Q61L) cells were treated with erastin (5 μM) combined with SP600125 (10 μM) and SB202190 (10 μM) for 48 h. TfR1 expression and the phosphorylation of p38 (p-P38) and JNK1/2 (p-JNK1/2) were assayed by western blot. 

HSPA5 / p-EIF2AK3; 

PubMed: 28130223     

Western blot analysis indicated protein expression in PDAC cells following treatment with erastin (2.5-40 μM) for 24 hours (n=3, *p < 0.05 versus untreated group). 


PubMed: 29383150     

Cells were treated with 50 μM erastin for various time(1-24 h). Whole-cell extracts were analyzed with immunoblotting assay.

27308510 31105999 28130223 29383150
Growth inhibition assay
Cell survival; 

PubMed: 29348676     

The A375 and G-361 human melanoma cells were treated with erastin (2.5-40 µM) or RSL3 (0.1-10 µM) with or without a cell death inhibitor (ferrostatin-1, 1 µM; ZVAD-FMK, 10 µM; necrosulfonamide, 0.5 µM) for 24 h. Cell death was assayed using a CCK-8 kit. Data shown represent mean ± SD from three independent experiments. ****p < 0.0001.


PubMed: 31105999     

HL-60/NRAS (Q61L) cells were treated with erastin (5 μM) with or without Fer-1 (1 μM) pretreatment for 48 h, and then the nuclear/cytosolic HMGB1 expression was assayed by immunofluorescence(Green, HMGB1; blue, nucleus). 



Cell Research:[1]
+ Expand
  • Cell lines: BJ-TERT/LT/ST/RASV12 cells
  • Concentrations: 5 or 10 μg/mL
  • Incubation Time: 6-11 hours
  • Method: BJ-TERT/LT/ST/RASV12 cells are seeded in 100 mm dishes and allowed to grow overnight. Cells are treated with erastin (5 or 10 μg/ml) for 6, 8, or 11 hr. A camptothecin-treated (0.4 μg/ml) control is maintained, treated at the time of seeding for 20 hours. After the treatment, cells are harvested with trypsin/EDTA and washed once with fresh medium containing serum and then twice with phosphate-buffered saline. Cells are resuspended in 1× binding buffer. 100 μL is incubated with 5 μL of Annexin V-FITC and propidium iodiode for 15 min in the dark at room temperature. Then 400 μl of the 1× binding buffer s added and the cells analyzed by flow cytometry. Data are acquired and analyzed using Cellquest software. Only viable cells that do not stain with propidium iodiode are analzyed for Annexin V-FITC staining using the FL1 channel.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 19 mg/mL (34.73 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 547.04


CAS No. 571203-78-6
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy Erastin | Erastin supplier | purchase Erastin | Erastin cost | Erastin manufacturer | order Erastin | Erastin distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID