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Catalog No.S7242

81 publications

Erastin Chemical Structure

CAS No. 571203-78-6

Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.

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Selleck's Erastin has been cited by 81 publications

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Biological Activity

Description Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.
Ferroptosis [1]
In vitro

Erastin is selectively lethal to oncogenic RAS-mutant cell lines, and triggers a unique iron-dependent form of non-apoptotic cell death called ferroptosis. [1] [2] Erastin binds directly to VDAC2 and causes mitochondrial damage via ROS production in an NADH-dependent manner, which induces cell death in some tumor cells harbouring activating mutations in the RAS-RAF-MEK pathway. [3] In addition, erastin, via inducing ROS-mediated CID (Caspase-independent cell death), strongly enhances the effect of cisplatin in WT EGFR cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human CCF-STTG1 cells NUWxZ3NyTnWwY4Tpc44h[XO|YYm= NIPZUHBKdmirYnn0bY9vKG:oIGjjeEBqdiCqdX3hckBES0ZvU2TUS|Eh[2WubIOgZZN{\XO|ZXSgZZMh\2y3dHHtZZRmKHKnbHXhd4Uh[W[2ZYKgNkBpenNiYomg[ox2d3KxbXX0dpktKEmFNUC9NE4zKM7:TT6= NGrPVIEzPjJ|MUG1Oi=>
human HeLa cells M1HxUmZ2dmO2aX;uJIF{e2G7 MXHJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCKZVzhJINmdGy|LDDFR|UxRTBwNjFOwG0v NWHpcJFGOTd3Nki3OFg>
human BJ cells MVXGeY5kfGmxbjDhd5NigQ>? NFq3d2RKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDCTkBk\WyuczDlfJBz\XO|aX7nJHRGWlRuIFzUMEBUXCCjbnSgVmFUKEdzMm[gcZV1[W62IHflcoV{KGOnbHzzJIlvKHC{ZYPlcoNmKG:oIGDEMVk5ODV7IHL5JJRzgXCjbjDicJVmKGW6Y3z1d4lwdiCvZYToc4QtKEmFNUC9NE46KM7:TT6= NF61SpAyPzV4OEe0PC=>
human HT1080 cells NH\CdYxHfW6ldHnvckBie3OjeR?= NHS3bWdKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDIWFExQDBiY3XscJMhcW5icILld4Vv[2Vib3[gVGQuQThyNUmgZpkhfHK7cHHuJIJtfWViZYjjcJV{cW:wIH3leIhw\CxiSVO1NF0yKM7:TT6= M3ToU|E4PTZ6N{S4
human SVR cells MVzGeY5kfGmxbjDhd5NigQ>? NFHhWlBKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDTWnIh[2WubIOsJGVEPTB;Mj61JO69VS5? MY[xO|U3QDd2OB?=
human MES-SA cells M1jWSmZ2dmO2aX;uJIF{e2G7 MWXJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCPRWOtV2Eh[2WubIOsJGVEPTB;MzFOwG0v Mn;iNVc2Pjh5NEi=
human SKUT cells NF\1UGxHfW6ldHnvckBie3OjeR?= NIW0bFZKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDTT3VVKGOnbHzzMEBGSzVyPUSg{txONg>? NVGwRW9vOTd3Nki3OFg>
human Calu1 cells MX3GeY5kfGmxbjDhd5NigQ>? NUjVTZNRUW6qaXLpeIlwdiCxZjDoeY1idiCFYXz1NUBk\WyuczDlfJBz\XO|aX7nJGtTSVNid3n0bEBi[3SrdnH0bY5oKG23dHH0bY9veyCkeTD0dplx[W5iYnz1[UBmgGOudYPpc44h[XO|YYmsJGlEPTB;NDFOwG0v M2DBPFE4PTZ6N{S4
human LNCaP cells MUDGeY5kfGmxbjDhd5NigQ>? Mn73TY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iTF7DZXAh[2WubIOsJGVEPTB;NjFOwG0v MYmxO|U3QDd2OB?=
human U2OS cells M{TX[2Z2dmO2aX;uJIF{e2G7 MXfJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCXMl;TJINmdGy|LDDFR|UxRTZizszNMi=> M3;wTlE4PTZ6N{S4
human TC32 cells NIHwPYdHfW6ldHnvckBie3OjeR?= M4rPU2lv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIGTDN|Ih[2WubIO= Ml;DNVc2Pjh5NEi=
human SK-N-MC cells NGW2c29HfW6ldHnvckBie3OjeR?= NIDL[WFKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDTT{1PNU2FIHPlcIx{NCCHQ{WwQVExKM7:TT6= MXSxO|U3QDd2OB?=
human U937 cells Mme4SpVv[3Srb36gZZN{[Xl? NXrsTVZVUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gWVk{PyClZXzsd{whTUN3ME2xNEDPxE1w M{LUN|E4PTZ6N{S4
human TC71 cells M2TOXmZ2dmO2aX;uJIF{e2G7 NELK[WZKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDUR|cyKGOnbHzzMEBGSzVyPUGwJO69VS5? NEfXU|QyPzV4OEe0PC=>
human BJ cells NF\JSJlHfW6ldHnvckBie3OjeR?= M2TsOWlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJHRGWlRiZYjwdoV{e2mwZzDoeY1idiCESjDj[YxteyxiRVO1NF0yOCEQvF2u NFzjXXkyPzV4OEe0PC=>
human EWS502 cells NVe1XHRQTnWwY4Tpc44h[XO|YYm= MX\JcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCHV2O1NFIh[2WubIOsJGVEPTB;MUCg{txONg>? NFyzb4oyPzV4OEe0PC=>
human Hs51.T cells NUHEcGdRTnWwY4Tpc44h[XO|YYm= MX3JcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCKc{WxMnQh[2WubIOsJGVEPTB;MUKg{txONg>? M1vNZlE4PTZ6N{S4
human Hs925.T cells M2rofGZ2dmO2aX;uJIF{e2G7 NXfr[IJXUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gTJM6OjVwVDDj[YxteyxiRVO1NF0yPyEQvF2u MmPZNVc2Pjh5NEi=
human HOS cells M1jEeGZ2dmO2aX;uJIF{e2G7 MnzjTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iSF;TJINmdGy|IDygSWM2OD1zNzFOwG0v NXnlWJlNOTd3Nki3OFg>
human MX2 cells MXjGeY5kfGmxbjDhd5NigQ>? MknqTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iTWiyJINmdGy|LDDFR|UxRTF6IN88UU4> NG\iS5YyPzV4OEe0PC=>
human A673 cells M4XjTGZ2dmO2aX;uJIF{e2G7 MV3JcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCDNkezJINmdGy|LDDFR|UxRTNyIN88UU4> NH;nRYsyPzV4OEe0PC=>
human BJ cells Mnu5SpVv[3Srb36gZZN{[Xl? M2jkNmlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFLKJINmdGy|IHX4dJJme3OrbnegWGVTXCxiTGSsJHNVKGGwZDDSRXMhTzF{VjDteZRidnRiZ3Xu[ZMhcW5icILld4Vv[2Vib3[gWVAyOjZiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKG2ndHjv[EwhUUN3ME2zNU4zKM7:TT6= NW\lPIZ{OTd3Nki3OFg>
human BJ cells MXnGeY5kfGmxbjDhd5NigQ>? M1LRfFkh|ryP M{\EcWlv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFLKJINmdGy|IHX4dJJme3OrbnegWGVTXCxiTGSsJHNVKGGwZDDSRXMhTzF{VjDteZRidnRiZ3Xu[ZMh[2WubIOgZZQhQSC3TT6= MUexO|U3QDd2OB?=
human BJ cells NIDKT2FHfW6ldHnvckBie3OjeR?= M3K4UVQvPiEQvF2= NFv6Z2FKdmO{ZXHz[UBqdiCrboTyZYNmdGy3bHHyJI95cWSjdHn2[UB{eGWlaXXzJIlvKGi3bXHuJGJLKGOnbHzzJIV5eHKnc4PpcochXEWUVDygUHQtKFOWIHHu[EBTSVNiR{GyWkBufXSjboSg[4Vv\XNiY3XscJMh[XRiND62JJVO MYKxO|U3QDd2OB?=
human BJeLR cells MULDfZRwfG:6aXRCpIF{e2G7 MonQNVAh|ryP M3rEOlEzKGh? MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCToVNWiClZXzsd{BmgHC{ZYPzbY5oKFKDUzDHNVJXKG23dHHueEBifCBzMDD1UUBifCBzMjDodpMh[nlidIL5dIFvKGKudXWgd5RicW6rbne= NHrJOlczOjh|MkOyNS=>
human BJeH cells NF3wRlBHfW6ldHnvckBie3OjeR?= M13CV|YhcA>? NXXWbop4UW6mdXP0bY9vKG:oIILlZYN1cX[nIH;4fYdmdiC|cHXjbYV{KHC{b3T1Z5Rqd25iaX6gbJVu[W5iQlrlTEBk\WyuczDlfJBz\XO|aX7nJJdqdGRidInw[UBTSVNiYX\0[ZIhPiCqcoOgZpkhTEOILXLhd4VlKG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=> M1;kV|IzQDN{M{Kx

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
GPX4 / cleaved-PARP / cleaved-caspase3 / LC3 / p62 / LDH / HMGB1; 

PubMed: 27308510     

HL-60 and Jurkat cells were treated with erastin (5 μM) for 24 h and subjected to western blot analysis of the indicated proteins in whole cell extracts or supernatant. 

TfR1 / p-JNK / JNK / p-P38; 

PubMed: 31105999     

HL-60/NRAS (Q61L) cells were treated with erastin (5 μM) combined with SP600125 (10 μM) and SB202190 (10 μM) for 48 h. TfR1 expression and the phosphorylation of p38 (p-P38) and JNK1/2 (p-JNK1/2) were assayed by western blot. 

HSPA5 / p-EIF2AK3; 

PubMed: 28130223     

Western blot analysis indicated protein expression in PDAC cells following treatment with erastin (2.5-40 μM) for 24 hours (n=3, *p < 0.05 versus untreated group). 


PubMed: 29383150     

Cells were treated with 50 μM erastin for various time(1-24 h). Whole-cell extracts were analyzed with immunoblotting assay.

27308510 31105999 28130223 29383150
Growth inhibition assay
Cell survival; 

PubMed: 29348676     

The A375 and G-361 human melanoma cells were treated with erastin (2.5-40 µM) or RSL3 (0.1-10 µM) with or without a cell death inhibitor (ferrostatin-1, 1 µM; ZVAD-FMK, 10 µM; necrosulfonamide, 0.5 µM) for 24 h. Cell death was assayed using a CCK-8 kit. Data shown represent mean ± SD from three independent experiments. ****p < 0.0001.


PubMed: 31105999     

HL-60/NRAS (Q61L) cells were treated with erastin (5 μM) with or without Fer-1 (1 μM) pretreatment for 48 h, and then the nuclear/cytosolic HMGB1 expression was assayed by immunofluorescence(Green, HMGB1; blue, nucleus). 



Cell Research:[1]
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  • Cell lines: BJ-TERT/LT/ST/RASV12 cells
  • Concentrations: 5 or 10 μg/mL
  • Incubation Time: 6-11 hours
  • Method: BJ-TERT/LT/ST/RASV12 cells are seeded in 100 mm dishes and allowed to grow overnight. Cells are treated with erastin (5 or 10 μg/ml) for 6, 8, or 11 hr. A camptothecin-treated (0.4 μg/ml) control is maintained, treated at the time of seeding for 20 hours. After the treatment, cells are harvested with trypsin/EDTA and washed once with fresh medium containing serum and then twice with phosphate-buffered saline. Cells are resuspended in 1× binding buffer. 100 μL is incubated with 5 μL of Annexin V-FITC and propidium iodiode for 15 min in the dark at room temperature. Then 400 μl of the 1× binding buffer s added and the cells analyzed by flow cytometry. Data are acquired and analyzed using Cellquest software. Only viable cells that do not stain with propidium iodiode are analzyed for Annexin V-FITC staining using the FL1 channel.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 19 mg/mL (34.73 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG 300+5%Tween80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 547.04


CAS No. 571203-78-6
Storage 3 years -20°C powder
1 month -80°C in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID