Home Metabolism Ferroptosis activator FIN56 For research use only.

FIN56

FIN56 is a specific inducer of ferroptosis .

FIN56 Chemical Structure

FIN56 Chemical Structure

CAS: 1083162-61-1

Selleck's FIN56 has been cited by 24 publications

Purity & Quality Control

Batch: S825401 DMSO] 100 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.28%
99.28

FIN56 Related Products

Choose Selective Ferroptosis Inhibitors

Biological Activity

Description FIN56 is a specific inducer of ferroptosis .
Targets
Ferroptosis [1]
In vitro
In vitro FIN56 is a specific inducer of ferroptosis. The mechanism involves two distinct pathways: one pathway is degradation of GPX4, which requires the enzymatic activity of acetyl-CoA carboxylase; the other pathway is activation of squalene synthase, which leads to coenzyme Q10 depletion, in a manner independent of GPX4 degradation[1].
Cell Research Cell lines HT-1080 cells
Concentrations 5 μM
Incubation Time 10 h
Method

500,000 HT-1080 cells are seeded in a 10-cm dish. Cells are grown at 37 °C for 16 h. On the day of the analysis, cells are cotreated with 100 μM α-tocopherol and either vehicle (DMSO) or a ferroptosis inducer (10 μM erastin, 0.5 μM (1S, 3R)-RSL3, or 5 μM FIN56) and incubated for 10 h. Cells are then trypsinized, pelleted, washed once with 400 μL of ice-cold PBS containing 1 mM EDTA, and sonicated. After the cell debris have been pelleted and removed, both oxidized and reduced glutathione in 120 μL of sample is quantified in technical triplicates. The glutathione quantity is normalized to the protein concentration measured via Bradford assay.

Chemical Information & Solubility

Molecular Weight 517.66 Formula

C25H31N3O5S2
CAS No. 1083162-61-1 SDF Download FIN56 SDF
Smiles C1CCC(CC1)NS(=O)(=O)C2=CC3=C(C=C2)C4=C(C3=NO)C=C(C=C4)S(=O)(=O)NC5CCCCC5
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (193.17 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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