SRS16-86

For research use only.

Catalog No.S9840

SRS16-86 Chemical Structure

CAS No. 1793052-96-6

SRS16-86, a novel third-generation ferrostatin, is an inhibitor of ferroptosis.

Purity & Quality Control

Choose Selective Ferroptosis Inhibitors

Biological Activity

Description SRS16-86, a novel third-generation ferrostatin, is an inhibitor of ferroptosis.
In vitro

SRS16-86 exhibits ferroptosis-inhibiting ability in vitro by FACS analysis of erastin-treated HT-1080 cells and in NIH 3T3 cells.[1]

In vivo

SRS16-86 (a novel third-generation ferrostatin) is more stable, to metabolism and plasma, and more potent, compared with the first-in-class compound ferrostatin-1 (Fer-1) to suppress ferroptosis in vivo. Even in conditions with extraordinarily severe IRI, SRS16-86 exerts strong protection to an extent which has not previously allowed survival in any murine setting.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: HT-1080 cells, NIH 3T3 cells
  • Concentrations: 1 μM
  • Incubation Time: 24 h, 40 h
  • Method:

    Phosphatidylserine exposure to the outer cell membrane of apoptotic cells or at the inner plasma membrane of necrotic cells and incorporation of 7-AAD into necrotic cells are quantified by fluorescence-activated cell sorting (FACS) analysis. FACS analysis is used for the necrotic marker 7AAD and phosphatidylserine accessibility (annexin V) in HT1080 and NIH 3T3 cells treated with either vehicle or 50 µM erastin in the presence or absence of 1 μM SRS16-86.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: 8- to 12-wk-old male C57BL/6 mice
  • Dosages: 2 mg/kg
  • Administration: IP
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 5 mg/mL (11.55 mM)
Water Insoluble
Ethanol ''2 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 432.56
Formula

C26H32N4O2

CAS No. 1793052-96-6
Storage powder
in solvent
Synonyms N/A

In vivo Formulation Calculator (Clear solution)

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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

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Dilution Calculator

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

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Molecular Weight Calculator

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Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID