Nocodazole

Catalog No.S2775 Batch:s277501

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Technical Data

Formula

C14H11N3O3S
Molecular Weight 301.32 CAS No. 31430-18-9
Solubility (25°C)* In vitro DMSO 7 mg/mL (23.23 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole induces apoptosis.
Targets
Microtubules [2]
(Cell-free assay)		 Abl [1]
(Cell-free assay)		 Abl (E255K) [1]
(Cell-free assay)		 Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
In vitro

Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. [1]

At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. [2]

Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. [3]

Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. [4]

Nocodazole doubles HDR efficiency to up to 30% in iPSCs. It improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing[6].
In vivo

The tumor volume and tumor weight of the mice treated with Ketoconazole  plus  Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole  plus  Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole  or  Nocodazole alone. [5]

Protocol (from reference)

Cell Assay:

[7]
  • Cell lines

    H1975 cells

  • Concentrations

    50 ng/ml

  • Incubation Time

    24 h

  • Method

    Cells were treated with indicated concentration of the drug for 24 hour.
Animal Study:

[5]
  • Animal Models

    Nude mice with COLO-205 tumor xenografts

  • Dosages

    5 mg/kg

  • Administration

    Administered via i.p.

Customer Product Validation

, , J Biol Chem, 2016, 291:14761-14772.

Data from [Data independently produced by , , Cancer Res, 2018, doi:10.1158/0008-5472.CAN-18-0520]

Data from [Data independently produced by , , Cell Signal, 2016, 28(12):1826-1832]

Data from [Data independently produced by , , J Biol Chem, 2017, 292(19):7806-7816]

Selleck's Nocodazole has been cited by 108 publications

Nucleolar protein TAAP1/C22orf46 confers pro-survival signaling in non-small cell lung cancer [ Life Sci Alliance, 2024, 7(4)e202302257] PubMed: 38228372
Allosteric regulation of CAD modulates de novo pyrimidine synthesis during the cell cycle [ Nat Metab, 2023, 5(2):277-293] PubMed: 36747088
Real-Time Dissection of the Transportation and miRNA-Release Dynamics of Small Extracellular Vesicles [ Adv Sci (Weinh), 2023, 10(7):e2205566] PubMed: 36599707
Harnessing transcriptionally driven chromosomal instability adaptation to target therapy-refractory lethal prostate cancer [ Cell Rep Med, 2023, 4(2):100937] PubMed: 36787737
Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma [ Dev Cell, 2023, 58(3):192-210.e11] PubMed: 36696903
Fatty acid oxidation facilitates DNA double-strand break repair by promoting PARP1 acetylation [ Cell Death Dis, 2023, 14(7):435] PubMed: 37454129
Targeting MCL-1 triggers DNA damage and an anti-proliferative response independent from apoptosis induction [ Cell Rep, 2023, 42(10):113176] PubMed: 37773750
Ferroptosis signaling promotes the release of misfolded proteins via exosomes to rescue ER stress in hepatocellular carcinoma [ Free Radic Biol Med, 2023, 202:110-120] PubMed: 36997100
PLK1 maintains DNA methylation and cell viability by regulating phosphorylation-dependent UHRF1 protein stability [ Cell Death Discov, 2023, 9(1):367] PubMed: 37788997
PLK1 maintains DNA methylation and cell viability by regulating phosphorylation-dependent UHRF1 protein stability [ Cell Death Discov, 2023, 9(1):367] PubMed: 37788997

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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