Monomethyl auristatin E (MMAE)

For research use only.

Catalog No.S7721

1 publication

Monomethyl auristatin E (MMAE) Chemical Structure

Molecular Weight(MW): 717.98

Monomethyl auristatin E (MMAE) is a synthetic antineoplastic agent, which is linked to a monoclonal antibody (MAB). It is also a microtubule-disrupting agent .

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Selleck's Monomethyl auristatin E (MMAE) has been cited by 1 publication

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Choose Selective ADC Cytotoxin Inhibitors

Biological Activity

Description Monomethyl auristatin E (MMAE) is a synthetic antineoplastic agent, which is linked to a monoclonal antibody (MAB). It is also a microtubule-disrupting agent .
In vitro

When coupled to cAC10, MMAE shows selective cytotoxicity in CD30+ cells, and induces G2/M-phase growth arrest and cell death through the induction of apoptosis. [1] When coupled to anti-CD79b antibody, anti–CD79b-vcMMAE has very potent and broad activity across a large panel of NHL cell lines in vitro. [2] When coupled to anti-HER2 antibody, hertuzumab-vc-MMAE can also be effectively internalized and potently kill HER2 over-expressing tumor cells. [3]

In vivo In the Karpas 299 ALCL model, cAC10-vcMMAE (1 mg/kg, i.v.) induces complete, durable tumor regression, while free MMAE (0.36 mg/kg) doesn’t produce detectable antitumor activity. [1] In mouse xenograft models of NHL, anti–CD79b-vcMMAE (7 mg/kg, p.o.) strikingly results in sustained complete tumor remission. [2]


Cell Research:


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  • Cell lines: CD30+ Karpas 299 cells
  • Concentrations: ~1000 ng/mL
  • Incubation Time: 96 h
  • Method:

    Cytotoxicity is measured using Alamar Blue dye reduction assay according to the manufacturer's directions. Briefly, a 40% solution (wt/vol) of Alamar Blue is freshly prepared in complete media just before cultures are added. Ninety-two hours after drug exposure, Alamar Blue solution is added to cells to constitute 10% culture volume. Cells are incubated for 4 hours, and dye reduction is measured on a Fusion HT fluorescent plate reader (Packard Instruments, Meriden, CT).

    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (139.27 mM)
Ethanol 100 mg/mL (139.27 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 717.98
CAS No. 474645-27-7
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04084366 Recruiting Drug: OBI-999 Locally Advanced Solid Tumor OBI Pharma Inc December 10 2019 Phase 1|Phase 2
NCT04042480 Recruiting Drug: SGN-CD228A Cutaneous Melanoma|Pleural Mesothelioma|Breast Cancer|Non-small Cell Lung Cancer|Colorectal Cancer|Pancreatic Ductal Adenocarcinoma Seattle Genetics Inc. September 3 2019 Phase 1
NCT03219333 Recruiting Drug: Enfortumab vedotin Carcinoma Transitional Cell|Urinary Bladder Neoplasms|Urologic Neoplasms|Renal Pelvis Neoplasms|Urothelial Cancer|Ureteral Neoplasms|Urethral Neoplasms Astellas Pharma Global Development Inc.|Seattle Genetics Inc.|Astellas Pharma Inc October 8 2017 Phase 2

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID