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Psoralen ADC Cytotoxin inhibitor

Cat.No.S4737

Psoralen (Psoralene, Ficusin, Furocoumarin) is a naturally occurring furocoumarin that intercalates with DNA, inhibiting DNA synthesis and cell division.
Psoralen ADC Cytotoxin inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 186.16

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Quality Control

Batch: Purity: 99.94%
99.94

Cell Culture, Treatment & Working Concentration

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LoVo Cytotoxicity assay Cytotoxicity against LoVo (human intestinal adenocarcinoma) cell line; Range 0.4-16 uM, IC50=16 μM
HL-60 Cytotoxicity assay Cytotoxicity against HL-60 (human fibrosarcoma) cell line; Range 0.4-16 uM, IC50=16 μM
HT-29 cells Cytotoxicity assay 96 h Cytotoxicity against human HT-29 cells after 96 hrs by MTT assay, IC50=41.7 μM
HCT116 cells Cytotoxicity assay 96 h Cytotoxicity against human HCT116 cells after 96 hrs by MTT assay, IC50=45.6 μM
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 37 mg/mL (198.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight
Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg
g
μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO
%
% Tween 80
% ddH2O
% DMSO
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%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 186.16 Formula

C11H6O3

 Storage (From the date of receipt)
CAS No. 66-97-7 Download SDF Storage of Stock Solutions

Synonyms Psoralene, Ficusin, Furocoumarin Smiles C1=CC(=O)OC2=CC3=C(C=CO3)C=C21

Mechanism of Action

In vitro
Psoralen inhibits the proliferation of MCF-7/ADR cells as shown by G0/G1 phase arrest rather than encouraging apoptosis. This compound reverses MDR(multidrug resistance) through inhibiting ATPase activity rather than reducing P-gp expression. It inhibits the migration abilities of MCF-7/ADR cells by repressing EMT possibly through inhibiting the activation of NF-κB. This chemical are photoactive compounds that readily alkylate DNA when activated by longwave ultraviolet light. Proliferation has been significantly promoted in MCF-7/ADR cells treated with low concentration of this compound (<10.75 µM) and inhibited with high concentration(>21.5 µM). It can inhibit metastasis of breast cancer. This compound mediates a variety of cell processes including cell death, proliferation, inflammation and migration.
In vivo
Psoralen has been characterized as a tumor suppressor in various tumors. This compound ameliorates sex hormone deficiency-induced osteoporosis in female and male mice. It has antiosteoporosis effect in ovariectomy-induced osteoporotic rats via stimulating osteoblastic differentiation from bone mesenchymal stem cells.
References

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