Tetrahydropalmatine hydrochloride

For research use only.

Catalog No.S3854 Synonyms: Gindarine hydrochloride, 1-Tetrahydropalmitine HCl

1 publication

Tetrahydropalmatine hydrochloride Chemical Structure

CAS No. 6024-85-7

Tetrahydropalmatine (THP, Gindarine, 1-Tetrahydropalmitine) is an isoquinoline alkaloid found in several different plant species, mainly in the Corydalis genus (Yan Hu Suo), but also in other plants such as Stephania rotunda. It is a potent muscle relaxant. Tetrahydropalmatine hydrochloride acts through inhibition of amygdaloid release of dopamine to inhibit an epileptic attack in rats.

Selleck's Tetrahydropalmatine hydrochloride has been cited by 1 publication

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Biological Activity

Description Tetrahydropalmatine (THP, Gindarine, 1-Tetrahydropalmitine) is an isoquinoline alkaloid found in several different plant species, mainly in the Corydalis genus (Yan Hu Suo), but also in other plants such as Stephania rotunda. It is a potent muscle relaxant. Tetrahydropalmatine hydrochloride acts through inhibition of amygdaloid release of dopamine to inhibit an epileptic attack in rats.
In vitro

Tetrahydropalmatine inhibits the LPSinduced production of COX-2 protein and mRNA in a dose-dependent manner. It suppresses activation of the NF-kB signaling pathway, which results in regulation of IL-8 expression and specifically blocks the ERK1=2 and p38 MAPK signaling pathways[1]. L-THP results in p53 independent apoptosis via down-regulating XIAP protein by inhibiting MDM2 associated with proteasome-dependent pathway and increased sensitivity of EU-4 cells against doxorubicin. L-THP causes activation of caspase and resulted in apoptosis[2]. l-THP possesses a blocking effect on dopamine D1 and D2 receptors and voltage-sensitive Ca2+ channels[3].

In vivo l-THP could attenuate the locomotor-stimulating effects of oxycodone and inhibit the development and expression of oxycodone behavioral sensitization[3].

Protocol

Cell Research:[1]
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  • Cell lines: THP-1 cells
  • Concentrations: 0.2, 1, or 2 mM
  • Incubation Time: 24 h
  • Method: Cell viability is examined by MTT assay. In brief, THP-1 cells are seeded at a density of 5×104 cells/mL in 96-well plates. Each batch of cells includes a nontreated group as a control. Tetrahydropalmatine (0.2, 1, or 2 mM) and LPS (2 mg/mL) are then added to each well, after which the plates are incubated for 24 hours at 37℃ under 5% CO2. Next, MTT solutions (5 mg/mL) are added to each well, and the samples are then cultured for an additional 4 hours. The supernatant is then discarded, and 100 mL of dimethylsulfoxide is added to each well. Next, the optical density is read at 590 nm.
    (Only for Reference)
Animal Research:[3]
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  • Animal Models: Kunming mice
  • Dosages: 6.25, 12.5, and 18.75 mg/kg
  • Administration: administered intragastrically
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 12 mg/mL (30.62 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 391.89
Formula

C21H25NO4.HCl

CAS No. 6024-85-7
Storage powder
in solvent
Synonyms Gindarine hydrochloride, 1-Tetrahydropalmitine HCl
Smiles COC1=C(C2=C(CC3C4=CC(=C(C=C4CCN3C2)OC)OC)C=C1)OC.Cl

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID