For research use only.

Catalog No.S3628 Synonyms: 2,5-di-t-butyl-1,4-benzohydroquinone

1 publication

BHQ Chemical Structure

CAS No. 88-58-4

BHQ (2,5-di-t-butyl-1,4-benzohydroquinone) is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA).

Selleck's BHQ has been cited by 1 publication

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Biological Activity

Description BHQ (2,5-di-t-butyl-1,4-benzohydroquinone) is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA).
66.7 μM
In vitro

2,5-Di-t-butyl-1,4-benzohydroquinone (BHQ) has been described as a rather selective sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitor. However, BHQ is not very specific, as it hits other sites of action besides SERCA. BHQ is shown, in fact, to reduce passive Ca2+ leakage from internal stores of permeabilized A7r5 vascular smooth muscle cells and to inhibit plasma membrane Ca2+ influx in parotid acinar cells, rat thymic lymphocytes, and GH3 pituitary cells. BHQ affects both endothelial and smooth muscle functions in rat aorta rings in vitro. BHQ induces endothelium-dependent relaxation by stimulating the NO synthase pathway, via activation of Ca2+ influx through Ni2+-sensitive Ca2+ channels in the endothelial cells. BHQ exhibits pleiotypic effects on rings deprived of endothelium by inducing both a myotonic response, dependent on the activation of Ca2+ influx via a Ni2+-sensitive pathway, and a myolytic effect, possibly dependent on either the depletion of intracellular Ca2+ stores or antagonism of Ca2+ entry via L-type Ca2+ channels. BHQ is a lipophilic, membrane permeable molecule, capable of generating superoxide anion at both sides of the plasmalemma[1].


Solubility (25°C)

In vitro Ethanol 40 mg/mL (179.92 mM)
DMSO 10 mg/mL (44.98 mM)
Water Insoluble

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Chemical Information

Molecular Weight 222.32


CAS No. 88-58-4
Storage powder
in solvent
Synonyms 2,5-di-t-butyl-1,4-benzohydroquinone
Smiles CC(C)(C)C1=CC(=C(C=C1O)C(C)(C)C)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03791086 Recruiting -- Bronchiectasis Adult University of Dundee November 1 2019 --
NCT01302886 Completed Drug: BHQ880 Smoldering Multiple Myeloma Novartis Pharmaceuticals|Novartis May 2011 Phase 2
NCT00741377 Completed Drug: BHQ880|Drug: Zoledronic acid Multiple Myeloma Bone Disease Novartis Pharmaceuticals|Novartis January 2009 Phase 1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID