For research use only.

Catalog No.S3628 Synonyms: 2,5-di-t-butyl-1,4-benzohydroquinone

BHQ Chemical Structure

Molecular Weight(MW): 222.32

BHQ is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA).

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Biological Activity

Description BHQ is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA).
66.7 μM
In vitro

2,5-Di-t-butyl-1,4-benzohydroquinone (BHQ) has been described as a rather selective sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitor. However, BHQ is not very specific, as it hits other sites of action besides SERCA. BHQ is shown, in fact, to reduce passive Ca2+ leakage from internal stores of permeabilized A7r5 vascular smooth muscle cells and to inhibit plasma membrane Ca2+ influx in parotid acinar cells, rat thymic lymphocytes, and GH3 pituitary cells. BHQ affects both endothelial and smooth muscle functions in rat aorta rings in vitro. BHQ induces endothelium-dependent relaxation by stimulating the NO synthase pathway, via activation of Ca2+ influx through Ni2+-sensitive Ca2+ channels in the endothelial cells. BHQ exhibits pleiotypic effects on rings deprived of endothelium by inducing both a myotonic response, dependent on the activation of Ca2+ influx via a Ni2+-sensitive pathway, and a myolytic effect, possibly dependent on either the depletion of intracellular Ca2+ stores or antagonism of Ca2+ entry via L-type Ca2+ channels. BHQ is a lipophilic, membrane permeable molecule, capable of generating superoxide anion at both sides of the plasmalemma[1].


Solubility (25°C)

In vitro Ethanol 40 mg/mL (179.92 mM)
DMSO 10 mg/mL (44.98 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 222.32


CAS No. 88-58-4
Storage powder
in solvent
Synonyms 2,5-di-t-butyl-1,4-benzohydroquinone
Smiles CC(C)(C)C1=C(O)C=C(C(=C1)O)C(C)(C)C

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID