For research use only.
Catalog No.S3628 Synonyms: 2,5-di-t-butyl-1,4-benzohydroquinone
CAS No. 88-58-4
BHQ (2,5-di-t-butyl-1,4-benzohydroquinone) is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA).
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|Description||BHQ (2,5-di-t-butyl-1,4-benzohydroquinone) is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA).|
2,5-Di-t-butyl-1,4-benzohydroquinone (BHQ) has been described as a rather selective sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitor. However, BHQ is not very specific, as it hits other sites of action besides SERCA. BHQ is shown, in fact, to reduce passive Ca2+ leakage from internal stores of permeabilized A7r5 vascular smooth muscle cells and to inhibit plasma membrane Ca2+ influx in parotid acinar cells, rat thymic lymphocytes, and GH3 pituitary cells. BHQ affects both endothelial and smooth muscle functions in rat aorta rings in vitro. BHQ induces endothelium-dependent relaxation by stimulating the NO synthase pathway, via activation of Ca2+ influx through Ni2+-sensitive Ca2+ channels in the endothelial cells. BHQ exhibits pleiotypic effects on rings deprived of endothelium by inducing both a myotonic response, dependent on the activation of Ca2+ influx via a Ni2+-sensitive pathway, and a myolytic effect, possibly dependent on either the depletion of intracellular Ca2+ stores or antagonism of Ca2+ entry via L-type Ca2+ channels. BHQ is a lipophilic, membrane permeable molecule, capable of generating superoxide anion at both sides of the plasmalemma.
|In vitro||Ethanol||40 mg/mL (179.92 mM)|
|DMSO||10 mg/mL (44.98 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
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|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
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|% DMSO % % Tween 80 % ddH2O|
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Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03791086||Recruiting||--||Bronchiectasis Adult||University of Dundee||November 1 2019||--|
|NCT01302886||Completed||Drug: BHQ880||Smoldering Multiple Myeloma||Novartis Pharmaceuticals|Novartis||May 2011||Phase 2|
|NCT00741377||Completed||Drug: BHQ880|Drug: Zoledronic acid||Multiple Myeloma Bone Disease||Novartis Pharmaceuticals|Novartis||January 2009||Phase 1|
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