Mitomycin C

Catalog No.S8146 Synonyms: Ametycine

For research use only.

Mitomycin C (Ametycine) is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.

Mitomycin C Chemical Structure

CAS No. 50-07-7

Selleck's Mitomycin C has been cited by 75 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Mitomycin C (Ametycine) is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.
Targets
DNA synthesis [1]
In vitro

Mitomycin C, by inducing DNA interstrand crosslinks, physically blocks DNA replication, recombination, and RNA transcription. [1] Mitomycin C potentiates TRAIL-induced apoptosis in HCT116 (p53-/-) colon cancer cells and sensitizes TRAIL-resistant colon cancer cells HT-29 to the cytokine by through JNK-independent upregulation of death receptors. [2] In different human cancer cell lines, such OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung) cells, Mitomycin C shows cytotoxic activity. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human CH1 (ovarian) tumor cell MYXDfZRwfG:6aXRCpIF{e2G7 NH\PVVNVcGViY3;tdI92dmRid3HzJIV3[Wy3YYTl[EBnd3JidHjlJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNJOSBqb4\hdolidilidIXtc5Ih[2WubDDsbY5meyxiSVO1NF01OCCwTR?= MojMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB4OUi0OFQoRjFyNkm4OFQ1RC:jPh?=
human HT-29 (colon) tumor cell NYX1[4puS3m2b4TvfIlkyqCjc4PhfS=> M3OwVHRp\SClb33wc5Vv\CC5YYOg[ZZidHWjdHXkJIZweiC2aHWgZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliKHPvcI9vMSC2dX3vdkBk\WyuIHzpcoV{NCCLQ{WwQVQxKG6P NVnrdGRCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2PVg1PDRpPkGwOlk5PDR2PD;hQi=>
human A2780 (ovarian) tumor cell NWPJZmdMS3m2b4TvfIlkyqCjc4PhfS=> M3\5NHRp\SClb33wc5Vv\CC5YYOg[ZZidHWjdHXkJIZweiC2aHWgZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBiKH;2ZZJq[W5rIIT1cY9zKGOnbHygcIlv\XNuIFnDOVA:PTBibl2= NEfCcJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zME[5PFQ1PCd-MUC2PVg1PDR:L3G+
human colon cancer cell line HCT15 M3;rXmN6fG:2b4jpZ:Kh[XO|YYm= NHnLPWY4OiCq MlrmTY4hfmm2cn:gZ5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iY3;sc44h[2GwY3XyJINmdGxibHnu[UBJS1RzNTDh[pRmeiB5MjDodkBw\iCmcoXnJIV5eG:|dYLlJIRmfGW{bXnu[YQh[nlidHjlJHNTSiCjc4PhfUwhUUN3ME23NEBvVQ>? NHrFVJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEm4NFY4Oid-MUS5PFA3PzJ:L3G+
Human Caki-1 Renal CellCarcinoma Cell MnHMSpVv[3Srb36gZZN{[Xl? MXe5OkBp M{iyPWlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IFj1cYFvKEOja3mtNUBT\W6jbDDD[YxtS2G{Y3nuc41iKEOnbHygUIlv\XNiKFPEO|AsMSCjdDC5OpRpKGixdYKsJIlkPTB;NECgcm0> NYK2bGZkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3OFMyPzhpPkG1O|Q{OTd6PD;hQi=>
SW480 colon cancer cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVHUN|BWUW5idnn0do8hcW6qaXLpeIlwdiCxZjDTW|Q5OCClb3zvckBk[W6lZYKgZ4VtdCCuaX7lMEBKSzVyPUegcm0> MorZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{MEO0PUc,OTl{MEO0PVww[T5?
WiDr colon cancer cell NF3RTWpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHLmTJpKdiC4aYTyc{BqdmirYnn0bY9vKG:oIGfpSJIh[2:ub36gZ4Fv[2W{IHPlcIwhdGmwZTygTWM2OD1zMDDuUS=> NFXy[5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKwN|Q6Lz5zOUKwN|Q6RC:jPh?=
HeLa NHnZWotHfW6ldHnvckBie3OjeR?= M4f1eGlvKH[rdILvJIFvfGmlZXzseYxieiCjY4Tpeol1gSCjZ3HpcpN1KEinTHGgZ4VtdHNwLDDJR|UxKD1iMD64NUDPxE1w NVjoc|FmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS5OVAyOSd-MUS5OVAyOTxxYU6=
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HT-29 M2H6PWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MnfGPVYhcHK| MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkC5PUDPxE1w MonWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5OES0PUc,Ojd5OES0PVww[T5?
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EMT6 NHLReFdHfW6ldHnvckBie3OjeR?= NHTaeJYyKGi{ MnjJR49ueG:3bnSgbZMhdWWjc4Xy[YQh\m:{IIP1dpZqfmmwZzDmdoFkfGmxboOgc4YhTU2WNjD0eY1weiClZXzsd{B1emWjdHXkJIlvKGO3bIT1doUhf2m2aDCoNE42OCC3TTmgZ49v[2WwdILheIlwdiCob4KgNUBpeiC3bnTldkBi\XKxYnnjJINwdmSrdHnvck4tKEWGNUCgQUAxNjdizszNMi=> NYn1VZhuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxN{S1NlY4PCd-N{S1NlY4PDxxYU6=
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SBC-5 NG\Cb3JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MofRTWM2OCB;IEKuOVg6KG6P MnPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
HT-3 MlTmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoK2TWM2OCB;IEKuPVI5KG6P M4TIVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
SIG-M5 NXTsT2pmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4K3W2lEPTBiPTCzMlUzPCCwTR?= NEm1NoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
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CHP-212 M2XyfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4HwZ2lEPTBiPTC0MlM3QCCwTR?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
P12-ICHIKAWA MormS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVvxUWRpUUN3MDC9JFQvPzh5IH7N NHLIVGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
HUTU-80 MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4LkcGlEPTBiPTC0Mlg{KG6P MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
P30-OHK NF6zPFVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoXTTWM2OCB;IESuPFQzKG6P M3XBN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
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COR-L279 MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1XpSWlEPTBiPTC1MlU1PSCwTR?= NF;5SJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
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MHH-CALL-2 MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2j2OWlEPTBiPTC2MlA3KG6P NEHHVo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
BV-173 MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NInZRXhKSzVyIE2gOk4xPjdibl2= NFS4UY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
NCI-H209 NW\wbIxnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUTaSZd6UUN3MDC9JFYvOTZ6IH7N MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
NCI-H460 Mo\jS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEXXVolKSzVyIE2gOk41PTJibl2= M4[wV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
DU-145 M{Xa[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVfYfZB6UUN3MDC9JFYvPTF7IH7N NWDUZ2lpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
769-P NEXDN3JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFO4TYRKSzVyIE2gO{4yOTJibl2= NE\t[nE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
ES1 NF7SWWlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXnGeW9VUUN3MDC9JFcvOjh6IH7N NXPKNW9iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
COLO-668 M1TRS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUDvRnVGUUN3MDC9JFcvPTR3IH7N MmfZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
NKM-1 MkKxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXW4dmdYUUN3MDC9JFcvQDNzIH7N NHPCdnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
ES3 Ml3uS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFfDO2xKSzVyIE2gO{45QDJibl2= MlrZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
SCC-3 NHPqZ3JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1LqemlEPTBiPTC4MlUyQSCwTR?= NX\6PHJZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
SU-DHL-1 MnztS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXLmbXRSUUN3MDC9JFgvPjB7IH7N MmjRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
ML-2 NIW1UVBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlX1TWM2OCB;IEiuO|gyKG6P Mnz2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
SF295 Mn\mS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3q5VGlEPTBiPTCxNU41QCCwTR?= NFryOWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
EW-7 NGTJdIpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4DJeGlEPTBiPTCxNk4yPCCwTR?= M{TLfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
H4 M{\xSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{T6XGlEPTBiPTCxNk41PCCwTR?= MnfQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
LB2241-RCC M3\KXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV3EbJZmUUN3MDC9JFE{NjF7IH7N M1z1d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
SR Mn:4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGW1bpJKSzVyIE2gNVMvPDZibl2= NFTaR|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
LB1047-RCC M13nOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYfJR|UxKD1iMUOuOkBvVQ>? M1nQTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
MCF7 MnPLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVThblM{UUN3MDC9JFE{Njlibl2= NHfGSXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
ESS-1 NEjpVVZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH6yTYpKSzVyIE2gNVMvQTJibl2= NYTxU401RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
NCI-H2122 NXj2bnN1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2TGfmlEPTBiPTCxN{46PiCwTR?= M4rhTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
786-0 NUnLPHRvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV3JR|UxKD1iMUSuNFEhdk1? NVrMV2p[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
COLO-205 MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mn\4TWM2OCB;IEG0MlM{KG6P MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
HAL-01 NXXq[GhET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFzXbIhKSzVyIE2gNVUvPzNibl2= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
MHH-ES-1 NUG3fohET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1;Y[mlEPTBiPTCxOk4zPiCwTR?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
NUGC-3 NVz2U5NIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHjL[mdKSzVyIE2gNVYvQTVibl2= M3vHR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
RPMI-8402 MkW2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHG2cY5KSzVyIE2gNVcvOTNibl2= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
ATN-1 NUn6XI9qT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGLEVmVKSzVyIE2gNVcvOzlibl2= NVfObWtGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
NCI-H441 Mnu1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIPYT2VKSzVyIE2gNVcvPiCwTR?= MmPPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
LB996-RCC NInuZnVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXf3PZdxUUN3MDC9JFE4NjZ6IH7N NUD0UYtZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
CAL-148 MnWyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmHvTWM2OCB;IEG3Mlg{KG6P MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
CTV-1 MlqzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGXQPZBKSzVyIE2gNVgvPjdibl2= NVTOcYRQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
HOP-62 NX\HZ4h2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUe0d49TUUN3MDC9JFIxNjJibl2= NInnbpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
CAKI-1 M3\Ed2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoPvTWM2OCB;IEKzMlMzKG6P NYG5XoZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
PA-1 M3nTXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVfrbWxmUUN3MDC9JFI{NjVzIH7N M{jqeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
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SK-N-FI MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2X6SmlEPTBiPTCzPU44ODd3IN88US=> NHHDeo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
CAL-72 NYSxdIU5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVfCeYxZUUN3MDC9JFQ3Njd|M{mg{txO NHr4S5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
Assay
Methods Test Index PMID
Western blot GRP78 / CHOP / Caspase-4 / Cleaved caspase-4 ; Beclin 1 / p62 / ATG5 ; p-Akt / Akt / p-S6K / S6K / p-Jun / JNK ; PARP / Caspase 8 / Caspase 9 / Cleaved caspase-9 / Caspase 3 / Cleaved caspase-3 28553347 29142609 24901052
In vivo

As the clinical first choice in superficial bladder tumors, in a rat bladder tumor model, Mitomycin C (400 μM) significantly prevents intravesical tumor growth. [4]

Protocol (from reference)

Animal Research:

[4]

  • Animal Models: Rat bladder tumor model
  • Dosages: 400 μM
  • Administration: Intravesical treatment

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 334.37
Formula

C15H18N4O5

CAS No. 50-07-7
Storage 3 years -20°C(in the dark) powder
2 years -80°C(in the dark) in solvent
Smiles CC1=C(C(=O)C2=C(C1=O)N3CC4C(C3(C2COC(=O)N)OC)N4)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04934540 Not yet recruiting Procedure: En bloc resection of bladder tumour Bladder Cancer Chinese University of Hong Kong October 1 2022 --
NCT02199327 Completed Drug: Mitomycin C|Drug: Interferon Alfa-2b Conjunctival Intraepithelial Neoplasia|Corneal Intraepithelial Neoplasia Coordinación de Investigación en Salud Mexico|Instituto Mexicano del Seguro Social|University of Guadalajara May 2014 Phase 4

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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