Mitomycin C

For research use only.

Catalog No.S8146

43 publications

Mitomycin C Chemical Structure

CAS No. 50-07-7

Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.

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Selleck's Mitomycin C has been cited by 43 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.
Targets
DNA synthesis [1]
In vitro

Mitomycin C, by inducing DNA interstrand crosslinks, physically blocks DNA replication, recombination, and RNA transcription. [1] Mitomycin C potentiates TRAIL-induced apoptosis in HCT116 (p53-/-) colon cancer cells and sensitizes TRAIL-resistant colon cancer cells HT-29 to the cytokine by through JNK-independent upregulation of death receptors. [2] In different human cancer cell lines, such OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung) cells, Mitomycin C shows cytotoxic activity. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human CH1 (ovarian) tumor cell Ml;PR5l1d3SxeHnjxsBie3OjeR?= NYO4fmtHXGinIHPvcZBwfW6mIIfhd{BmfmGudXH0[YQh\m:{IITo[UBkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBEUDFiKH;2ZZJq[W5rIIT1cY9zKGOnbHygcIlv\XNuIFnDOVA:PDBibl2= NXfheFF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2PVg1PDRpPkGwOlk5PDR2PD;hQi=>
human HT-29 (colon) tumor cell M4LDWmN6fG:2b4jpZ:Kh[XO|YYm= M1XBZnRp\SClb33wc5Vv\CC5YYOg[ZZidHWjdHXkJIZweiC2aHWgZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliKHPvcI9vMSC2dX3vdkBk\WyuIHzpcoV{NCCLQ{WwQVQxKG6P NILHSHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zME[5PFQ1PCd-MUC2PVg1PDR:L3G+
human A2780 (ovarian) tumor cell MlT3R5l1d3SxeHnjxsBie3OjeR?= MYrUbIUh[2:vcH;1coQhf2G|IHX2ZYx2[XSnZDDmc5IhfGinIHP5eI91d3irY3n0fUBi\2GrboP0JIh2dWGwIFGyO|gxKCixdnHybYFvMSC2dX3vdkBk\WyuIHzpcoV{NCCLQ{WwQVUxKG6P NWLscZNZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2PVg1PDRpPkGwOlk5PDR2PD;hQi=>
human colon cancer cell line HCT15 NX3XVJI2S3m2b4TvfIlkyqCjc4PhfS=> M{nmdVczKGh? NYHYNHdSUW5idnn0do8h[3m2b4TvfIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gZ49td25iY3HuZ4VzKGOnbHygcIlv\SCKQ2SxOUBi\nSncjC3NkBpeiCxZjDkdpVoKGW6cH;zeZJmKGSndHXycYlv\WRiYomgeIhmKFOUQjDhd5NigSxiSVO1NF04OCCwTR?= NXL3OY9KRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS5PFA3PzJpPkG0PVgxPjd{PD;hQi=>
Human Caki-1 Renal CellCarcinoma Cell MVjGeY5kfGmxbjDhd5NigQ>? NUXFN4NkQTZiaB?= NWT4TWM5UW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgTJVu[W5iQ3HrbU0yKFKnbnHsJGNmdGyFYYLjbY5wdWFiQ3XscEBNcW6nczCoR2Q4OCtrIHH0JFk3fGhiaH;1dkwhcWN3ME20NEBvVQ>? NVLaWmNvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3OFMyPzhpPkG1O|Q{OTd6PD;hQi=>
SW480 colon cancer cell NFj4e2FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXzJckB3cXS{bzDpcohq[mm2aX;uJI9nKFOZNEiwJINwdG:wIHPhcoNmeiClZXzsJIxqdmVuIFnDOVA:PyCwTR?= NVuwWWVXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmyNFM1QSd-MUmyNFM1QTxxYU6=
WiDr colon cancer cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWfJckB3cXS{bzDpcohq[mm2aX;uJI9nKFerRIKgZ49td25iY3HuZ4VzKGOnbHygcIlv\SxiSVO1NF0yOCCwTR?= NFLVd4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKwN|Q6Lz5zOUKwN|Q6RC:jPh?=
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P388 NXzDZVRiSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NEDsfGQ1QCCqcoO= M4Xx[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViUEO4PEBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMzFOwG0v NYXQb4d[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3PFQ1QSd-Mke3PFQ1QTxxYU6=
AA8 MYDGeY5kfGmxbjDhd5NigQ>? NVrNWG8xPCCqcoO= M{XQbmlvcGmkaYTvdpkh[WO2aY\peJkhd2ZiY3;tdI92dmRiZn;yJGFCQCClZXzsd{B1dyC{ZXT1Z4Uh[2WubDDk[Y5{cXS7IHL5JFUxLSBqZYjwc5Nm\CC2bzDjc41xd3WwZDDmc5IhPCCqb4Xyd{ktKEmFNUCgQUAxNjByMUC4JO69VS5? M1vhRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEm3OFEoRjJ7MEm3OFE9N2F-
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HeLa S3 Mon2SpVv[3Srb36gZZN{[Xl? MkT0TY4hfmm2cn:gZY51cWOnbHz1cIFzKGGldHn2bZR6KGGpYXnud5QhUGWOYTDTN{Bk\Wyuc{ugNE42QS1zLkGsJGlEPTBiPTCxMlEh|ryPLh?= NVnsNGJERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOECyNVkyQCd-OECyNVkyQDxxYU6=
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BV-173 MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIrOeVRKSzVyIE2gOk4xPjdibl2= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
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COLO-668 NIrscXdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkXkTWM2OCB;IEeuOVQ2KG6P M{XRUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
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LB2241-RCC MkSxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NY[2do5zUUN3MDC9JFE{NjF7IH7N M{LIeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
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LB1047-RCC NX7ncmhZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVLsOldUUUN3MDC9JFE{NjZibl2= NXz2RYpFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
MCF7 NU\hV5pET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NET5bYVKSzVyIE2gNVMvQSCwTR?= NGTKWVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
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786-0 M4fFVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4n6WGlEPTBiPTCxOE4xOSCwTR?= NHjnR289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
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HAL-01 M2nxbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH34NXlKSzVyIE2gNVUvPzNibl2= NXXueFlDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
MHH-ES-1 M4DYXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmfOTWM2OCB;IEG2MlI3KG6P MlTDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
NUGC-3 M4LFRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{DKcWlEPTBiPTCxOk46PSCwTR?= NVLWU2tvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
RPMI-8402 NHfwWJdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX;HfHRGUUN3MDC9JFE4NjF|IH7N NXLIWGJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
ATN-1 NX7UVZpCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVv2TIlVUUN3MDC9JFE4NjN7IH7N NHTNOIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
NCI-H441 M1rmSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M32xZmlEPTBiPTCxO{43KG6P MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
LB996-RCC NXPle2Z[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmDDTWM2OCB;IEG3MlY5KG6P M{XLXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
CAL-148 MmPOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2D3RmlEPTBiPTCxO{45OyCwTR?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
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HOP-62 NEizWIlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoHoTWM2OCB;IEKwMlIhdk1? M3fZZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
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PA-1 MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2f3VmlEPTBiPTCyN{42OSCwTR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
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MKN7 M4PWSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWfJR|UxKD1iMkeuPFU3OiEQvF2= NYDi[JFqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
SK-N-FI M{G3R2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3n4NGlEPTBiPTCzPU44ODd3IN88US=> M3TIWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
CAL-72 MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mki4TWM2OCB;IES2Mlc{OzlizszN M2e4cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
PARP / Caspase 8 / Caspase 9 / Cleaved caspase-9 / Caspase 3 / Cleaved caspase-3; 

PubMed: 24901052     


LS174T cells were treated with mitomycin C (5 μg/ml) and/or rapamycin (2.5–10 μg/ml) for 24 h. After treatment, the cleavage of caspase 8, caspase 9, caspase 3, or PARP was detected by immunoblotting.

p-Akt / Akt / p-S6K / S6K / p-Jun / JNK; 

PubMed: 24901052     


LS174T cells were treated with mitomycin C (5 μg/ml) and/or rapamycin (2.5–10 μg/ml) for 24 h and p-Akt/Akt, p-S6K1/S6K1 and p-JNK/JNK were detected by immunoblotting. Actin was used to confirm the equal amount of proteins loaded in each lane.

Beclin 1 / p62 / ATG5; 

PubMed: 29142609     


(A) Beclin 1, p62 and ATG5 expression levels in T24 cells were measured by western blotting. 1 is the control, 2 MMC only, 3 MMC + 0.25 µmol/l, 4 MMC + 1 µmol/l and 5 MMC + 10 µmol/l 5-Aza-2′ deoxycitidine. GAPDH was used as an internal control. MMC: Mitomycin C

GRP78 / CHOP / Caspase-4 / Cleaved caspase-4; 

PubMed: 28553347     


(A) Dose-dependent effect of MMC on endoplasmic reticulum stress-associated proteins. Cells were treated with various concentrations of MMC (5, 10, 20 μg/mL) or phosphate buffered saline for 24 hours and immunoblot analysis was performed with antibodies specific for GRP78, Caspase-4, CHOP and GAPDH (control).

24901052 29142609 28553347
In vivo As the clinical first choice in superficial bladder tumors, in a rat bladder tumor model, Mitomycin C (400 μM) significantly prevents intravesical tumor growth. [4]

Protocol

Animal Research:[4]
- Collapse
  • Animal Models: Rat bladder tumor model
  • Dosages: 400 μM
  • Administration: Intravesical treatment
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 66 mg/mL (197.38 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 334.37
Formula

C15H18N4O5

CAS No. 50-07-7
Storage 3 years -20°C(in the dark) powder
2 years -80°C(in the dark) in solvent
Synonyms N/A
Smiles CC1=C(C(=O)C2=C(C1=O)N3CC4C(C3(C2COC(=O)N)OC)N4)N

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02199327 Completed Drug: Mitomycin C|Drug: Interferon Alfa-2b Conjunctival Intraepithelial Neoplasia|Corneal Intraepithelial Neoplasia Coordinación de Investigación en Salud Mexico|Instituto Mexicano del Seguro Social|University of Guadalajara May 2014 Phase 4
NCT02015650 Terminated Drug: Cetuximab|Drug: Mitomycin-C/ 5-Fluorouracil Head and Neck Neoplasms Medical University Innsbruck April 2014 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID