Mitomycin C

For research use only.

Catalog No.S8146

55 publications

Mitomycin C Chemical Structure

CAS No. 50-07-7

Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.

Selleck's Mitomycin C has been cited by 55 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.
Targets
DNA synthesis [1]
In vitro

Mitomycin C, by inducing DNA interstrand crosslinks, physically blocks DNA replication, recombination, and RNA transcription. [1] Mitomycin C potentiates TRAIL-induced apoptosis in HCT116 (p53-/-) colon cancer cells and sensitizes TRAIL-resistant colon cancer cells HT-29 to the cytokine by through JNK-independent upregulation of death receptors. [2] In different human cancer cell lines, such OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung) cells, Mitomycin C shows cytotoxic activity. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human CH1 (ovarian) tumor cell NHPPbFVEgXSxdH;4bYPDqGG|c3H5 NFq4W4lVcGViY3;tdI92dmRid3HzJIV3[Wy3YYTl[EBnd3JidHjlJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNJOSBqb4\hdolidilidIXtc5Ih[2WubDDsbY5meyxiSVO1NF01OCCwTR?= NWnSR4luRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2PVg1PDRpPkGwOlk5PDR2PD;hQi=>
human HT-29 (colon) tumor cell M321e2N6fG:2b4jpZ:Kh[XO|YYm= Ml6xWIhmKGOxbYDveY5lKHejczDleoFtfWG2ZXSg[o9zKHSqZTDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSBqY3;sc44qKHS3bX;yJINmdGxibHnu[ZMtKEmFNUC9OFAhdk1? NYrPNIZ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2PVg1PDRpPkGwOlk5PDR2PD;hQi=>
human A2780 (ovarian) tumor cell NV7n[HdTS3m2b4TvfIlkyqCjc4PhfS=> MYXUbIUh[2:vcH;1coQhf2G|IHX2ZYx2[XSnZDDmc5IhfGinIHP5eI91d3irY3n0fUBi\2GrboP0JIh2dWGwIFGyO|gxKCixdnHybYFvMSC2dX3vdkBk\WyuIHzpcoV{NCCLQ{WwQVUxKG6P MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODZ7OES0OEc,OTB4OUi0OFQ9N2F-
human colon cancer cell line HCT15 NGqyOodEgXSxdH;4bYPDqGG|c3H5 MknXO|IhcA>? Mn7jTY4hfmm2cn:gZ5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iY3;sc44h[2GwY3XyJINmdGxibHnu[UBJS1RzNTDh[pRmeiB5MjDodkBw\iCmcoXnJIV5eG:|dYLlJIRmfGW{bXnu[YQh[nlidHjlJHNTSiCjc4PhfUwhUUN3ME23NEBvVQ>? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDl6ME[3Nkc,OTR7OEC2O|I9N2F-
Human Caki-1 Renal CellCarcinoma Cell NXH4SXRETnWwY4Tpc44h[XO|YYm= NGDjXpI6PiCq NFrKXZFKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDIeY1idiCFYXvpMVEhWmWwYXygR4VtdEOjcnPpco9u[SCFZXzsJGxqdmW|IDjDSFcxMyliYYSgPVZ1cCCqb4XyMEBq[zVyPUSwJI5O MmfnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5NEOxO|goRjF3N{SzNVc5RC:jPh?=
SW480 colon cancer cell M3Ticmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYnFOW0zUW5idnn0do8hcW6qaXLpeIlwdiCxZjDTW|Q5OCClb3zvckBk[W6lZYKgZ4VtdCCuaX7lMEBKSzVyPUegcm0> MoXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{MEO0PUc,OTl{MEO0PVww[T5?
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COLO-668 NYTZPWtXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoXITWM2OCB;IEeuOVQ2KG6P NX\pfVNmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
NKM-1 M{W2VGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXXYb2N4UUN3MDC9JFcvQDNzIH7N M1\ielxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
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LB2241-RCC Mmm2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXrJR|UxKD1iMUOuNVkhdk1? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
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LB1047-RCC MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVnnV49EUUN3MDC9JFE{NjZibl2= M1nqOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
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LB996-RCC Mn3PS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3HsdGlEPTBiPTCxO{43QCCwTR?= NHjzXFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
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MKN7 MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnH0TWM2OCB;IEK3Mlg2PjJizszN MnLLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
SK-N-FI MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXrJR|UxKD1iM{muO|A4PSEQvF2= MmnBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
CAL-72 Mlf0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmXTTWM2OCB;IES2Mlc{OzlizszN NFHrTHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
GRP78 / CHOP / Caspase-4 / Cleaved caspase-4; 

PubMed: 28553347     


(A) Dose-dependent effect of MMC on endoplasmic reticulum stress-associated proteins. Cells were treated with various concentrations of MMC (5, 10, 20 μg/mL) or phosphate buffered saline for 24 hours and immunoblot analysis was performed with antibodies specific for GRP78, Caspase-4, CHOP and GAPDH (control).

Beclin 1 / p62 / ATG5; 

PubMed: 29142609     


(A) Beclin 1, p62 and ATG5 expression levels in T24 cells were measured by western blotting. 1 is the control, 2 MMC only, 3 MMC + 0.25 µmol/l, 4 MMC + 1 µmol/l and 5 MMC + 10 µmol/l 5-Aza-2′ deoxycitidine. GAPDH was used as an internal control. MMC: Mitomycin C

p-Akt / Akt / p-S6K / S6K / p-Jun / JNK; 

PubMed: 24901052     


LS174T cells were treated with mitomycin C (5 μg/ml) and/or rapamycin (2.5–10 μg/ml) for 24 h and p-Akt/Akt, p-S6K1/S6K1 and p-JNK/JNK were detected by immunoblotting. Actin was used to confirm the equal amount of proteins loaded in each lane.

PARP / Caspase 8 / Caspase 9 / Cleaved caspase-9 / Caspase 3 / Cleaved caspase-3; 

PubMed: 24901052     


LS174T cells were treated with mitomycin C (5 μg/ml) and/or rapamycin (2.5–10 μg/ml) for 24 h. After treatment, the cleavage of caspase 8, caspase 9, caspase 3, or PARP was detected by immunoblotting.

28553347 29142609 24901052
In vivo As the clinical first choice in superficial bladder tumors, in a rat bladder tumor model, Mitomycin C (400 μM) significantly prevents intravesical tumor growth. [4]

Protocol

Animal Research:[4]
- Collapse
  • Animal Models: Rat bladder tumor model
  • Dosages: 400 μM
  • Administration: Intravesical treatment
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 66 mg/mL (197.38 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 334.37
Formula

C15H18N4O5

CAS No. 50-07-7
Storage 3 years -20°C(in the dark) powder
2 years -80°C(in the dark) in solvent
Synonyms N/A
Smiles CC1=C(C(=O)C2=C(C1=O)N3CC4C(C3(C2COC(=O)N)OC)N4)N

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Molarity Calculator

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Molecular Weight Calculator

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04934540 Not yet recruiting Procedure: En bloc resection of bladder tumour Bladder Cancer Chinese University of Hong Kong September 1 2021 --
NCT02199327 Completed Drug: Mitomycin C|Drug: Interferon Alfa-2b Conjunctival Intraepithelial Neoplasia|Corneal Intraepithelial Neoplasia Coordinación de Investigación en Salud Mexico|Instituto Mexicano del Seguro Social|University of Guadalajara May 2014 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID