Mitomycin C

Catalog No.S8146

For research use only.

Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.

Mitomycin C Chemical Structure

CAS No. 50-07-7

Selleck's Mitomycin C has been cited by 67 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.
Targets
DNA synthesis [1]
In vitro

Mitomycin C, by inducing DNA interstrand crosslinks, physically blocks DNA replication, recombination, and RNA transcription. [1] Mitomycin C potentiates TRAIL-induced apoptosis in HCT116 (p53-/-) colon cancer cells and sensitizes TRAIL-resistant colon cancer cells HT-29 to the cytokine by through JNK-independent upregulation of death receptors. [2] In different human cancer cell lines, such OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung) cells, Mitomycin C shows cytotoxic activity. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human CH1 (ovarian) tumor cell M{nrfWN6fG:2b4jpZ:Kh[XO|YYm= MortWIhmKGOxbYDveY5lKHejczDleoFtfWG2ZXSg[o9zKHSqZTDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDTFEhMG:4YYLpZY4qKHS3bX;yJINmdGxibHnu[ZMtKEmFNUC9OFAhdk1? M37CcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNkm4OFQ1Lz5zME[5PFQ1PDxxYU6=
human HT-29 (colon) tumor cell NXr5R|NWS3m2b4TvfIlkyqCjc4PhfS=> MmH4WIhmKGOxbYDveY5lKHejczDleoFtfWG2ZXSg[o9zKHSqZTDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSBqY3;sc44qKHS3bX;yJINmdGxibHnu[ZMtKEmFNUC9OFAhdk1? NFzMUIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zME[5PFQ1PCd-MUC2PVg1PDR:L3G+
human A2780 (ovarian) tumor cell M3XYT2N6fG:2b4jpZ:Kh[XO|YYm= NIDHfmtVcGViY3;tdI92dmRid3HzJIV3[Wy3YYTl[EBnd3JidHjlJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIDjveoFzcWGwKTD0eY1weiClZXzsJIxqdmW|LDDJR|UxRTVyIH7N NFPOT4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zME[5PFQ1PCd-MUC2PVg1PDR:L3G+
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MKN7 M1PScGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXvJR|UxKD1iMkeuPFU3OiEQvF2= NHTwSW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
SK-N-FI NGLOVppIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIXBd2xKSzVyIE2gN|kvPzB5NTFOwG0> M4Hm[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
CAL-72 NFLMRmtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXvJR|UxKD1iNE[uO|M{QSEQvF2= M1rLXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
Assay
Methods Test Index PMID
Western blot GRP78 / CHOP / Caspase-4 / Cleaved caspase-4 ; Beclin 1 / p62 / ATG5 ; p-Akt / Akt / p-S6K / S6K / p-Jun / JNK ; PARP / Caspase 8 / Caspase 9 / Cleaved caspase-9 / Caspase 3 / Cleaved caspase-3 28553347 29142609 24901052
In vivo As the clinical first choice in superficial bladder tumors, in a rat bladder tumor model, Mitomycin C (400 μM) significantly prevents intravesical tumor growth. [4]

Protocol (from reference)

Animal Research:[4]
  • Animal Models: Rat bladder tumor model
  • Dosages: 400 μM
  • Administration: Intravesical treatment

Solubility (25°C)

In vitro

DMSO 66 mg/mL
(197.38 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 334.37
Formula

C15H18N4O5

CAS No. 50-07-7
Storage 3 years -20°C(in the dark) powder
2 years -80°C(in the dark) in solvent
Smiles CC1=C(C(=O)C2=C(C1=O)N3CC4C(C3(C2COC(=O)N)OC)N4)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04934540 Not yet recruiting Procedure: En bloc resection of bladder tumour Bladder Cancer Chinese University of Hong Kong March 1 2022 --
NCT02199327 Completed Drug: Mitomycin C|Drug: Interferon Alfa-2b Conjunctival Intraepithelial Neoplasia|Corneal Intraepithelial Neoplasia Coordinación de Investigación en Salud Mexico|Instituto Mexicano del Seguro Social|University of Guadalajara May 2014 Phase 4

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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