Mitomycin C

For research use only.

Catalog No.S8146

46 publications

Mitomycin C Chemical Structure

CAS No. 50-07-7

Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.

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Selleck's Mitomycin C has been cited by 46 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.
Targets
DNA synthesis [1]
In vitro

Mitomycin C, by inducing DNA interstrand crosslinks, physically blocks DNA replication, recombination, and RNA transcription. [1] Mitomycin C potentiates TRAIL-induced apoptosis in HCT116 (p53-/-) colon cancer cells and sensitizes TRAIL-resistant colon cancer cells HT-29 to the cytokine by through JNK-independent upregulation of death receptors. [2] In different human cancer cell lines, such OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung) cells, Mitomycin C shows cytotoxic activity. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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human A2780 (ovarian) tumor cell MVjDfZRwfG:6aXRCpIF{e2G7 NFza[JpVcGViY3;tdI92dmRid3HzJIV3[Wy3YYTl[EBnd3JidHjlJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIDjveoFzcWGwKTD0eY1weiClZXzsJIxqdmW|LDDJR|UxRTVyIH7N NFPQfYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zME[5PFQ1PCd-MUC2PVg1PDR:L3G+
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COR-L279 NHXndYJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUTYeXU2UUN3MDC9JFUvPTR3IH7N NX3r[o9QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
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MHH-CALL-2 NIXnT|RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHnRd4VKSzVyIE2gOk4xPiCwTR?= MlfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
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COLO-668 MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX:3U5dQUUN3MDC9JFcvPTR3IH7N MkXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
NKM-1 NVrWTXlKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4XFdWlEPTBiPTC3Mlg{OSCwTR?= NEXQPGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
ES3 MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3LpbWlEPTBiPTC3Mlg5OiCwTR?= MljnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
SCC-3 NITweplIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH\HWpJKSzVyIE2gPE42OTlibl2= NF;i[HM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
SU-DHL-1 NF7tVIFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV;JR|UxKD1iOD62NFkhdk1? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
ML-2 NVTUcVlLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Moe2TWM2OCB;IEiuO|gyKG6P MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
SF295 NVnmRWROT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3H5dGlEPTBiPTCxNU41QCCwTR?= NUnJOnRxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
EW-7 MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIjIenhKSzVyIE2gNVIvOTRibl2= M3HBO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
H4 NH[zRVhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mly0TWM2OCB;IEGyMlQ1KG6P NIrYXZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
LB2241-RCC NYPsOW4zT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFLrUFFKSzVyIE2gNVMvOTlibl2= NGW1O2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
SR NIDUW|hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MknJTWM2OCB;IEGzMlQ3KG6P NHjWWXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
LB1047-RCC NIfTNZlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYPJR|UxKD1iMUOuOkBvVQ>? M2ThTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
MCF7 NFPzNItIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHvQRmZKSzVyIE2gNVMvQSCwTR?= NFPSNo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
ESS-1 M{fkSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWmyO41MUUN3MDC9JFE{Njl{IH7N NHe5Z3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
NCI-H2122 M{D5PWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3nvNGlEPTBiPTCxN{46PiCwTR?= NH;pbmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
786-0 MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1rtZmlEPTBiPTCxOE4xOSCwTR?= M2\RUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
COLO-205 MnX6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUHJR|UxKD1iMUSuN|Mhdk1? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
HAL-01 NX\sbHMyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWS3cI14UUN3MDC9JFE2Njd|IH7N NFT4Zo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
MHH-ES-1 MnvSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4nZTmlEPTBiPTCxOk4zPiCwTR?= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
NUGC-3 M3izT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYnKd3F4UUN3MDC9JFE3Njl3IH7N MoDpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
RPMI-8402 MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX3JR|UxKD1iMUeuNVMhdk1? NX7ESotCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
ATN-1 MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYL6fHRrUUN3MDC9JFE4NjN7IH7N NVPL[3ZURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
NCI-H441 NVPHUnBFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYXiNG9kUUN3MDC9JFE4NjZibl2= NVH0dVRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
LB996-RCC NX72Z29XT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWPJR|UxKD1iMUeuOlghdk1? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFUwLz6VQV7HSXI9N2F-
CAL-148 MofQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NY\kcWxpUUN3MDC9JFE4Njh|IH7N NV7jTWVoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
CTV-1 M1jOd2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWfMc4tUUUN3MDC9JFE5NjZ5IH7N NVzGRo8zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
HOP-62 NYDicZVuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlrwTWM2OCB;IEKwMlIhdk1? NH\yTmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODVxJ{7TRW5ITVJ:L3G+
CAKI-1 MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3zibmlEPTBiPTCyN{4{OiCwTR?= NUjyeWMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3Lze+V2FPT0WUPD;hQi=>
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MKN7 NIPndZZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NI\RcItKSzVyIE2gNlcvQDV4MjFOwG0> MoHyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2Nyd-U1HOS2VTRC:jPh?=
SK-N-FI M2X5fWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYDqUGMzUUN3MDC9JFM6NjdyN{Wg{txO M4POVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=
CAL-72 M3vSbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4Oye2lEPTBiPTC0Ok44OzN7IN88US=> M131WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPS9pPmPBUmdGWjxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
PARP / Caspase 8 / Caspase 9 / Cleaved caspase-9 / Caspase 3 / Cleaved caspase-3; 

PubMed: 24901052     


LS174T cells were treated with mitomycin C (5 μg/ml) and/or rapamycin (2.5–10 μg/ml) for 24 h. After treatment, the cleavage of caspase 8, caspase 9, caspase 3, or PARP was detected by immunoblotting.

p-Akt / Akt / p-S6K / S6K / p-Jun / JNK; 

PubMed: 24901052     


LS174T cells were treated with mitomycin C (5 μg/ml) and/or rapamycin (2.5–10 μg/ml) for 24 h and p-Akt/Akt, p-S6K1/S6K1 and p-JNK/JNK were detected by immunoblotting. Actin was used to confirm the equal amount of proteins loaded in each lane.

Beclin 1 / p62 / ATG5; 

PubMed: 29142609     


(A) Beclin 1, p62 and ATG5 expression levels in T24 cells were measured by western blotting. 1 is the control, 2 MMC only, 3 MMC + 0.25 µmol/l, 4 MMC + 1 µmol/l and 5 MMC + 10 µmol/l 5-Aza-2′ deoxycitidine. GAPDH was used as an internal control. MMC: Mitomycin C

GRP78 / CHOP / Caspase-4 / Cleaved caspase-4; 

PubMed: 28553347     


(A) Dose-dependent effect of MMC on endoplasmic reticulum stress-associated proteins. Cells were treated with various concentrations of MMC (5, 10, 20 μg/mL) or phosphate buffered saline for 24 hours and immunoblot analysis was performed with antibodies specific for GRP78, Caspase-4, CHOP and GAPDH (control).

24901052 29142609 28553347
In vivo As the clinical first choice in superficial bladder tumors, in a rat bladder tumor model, Mitomycin C (400 μM) significantly prevents intravesical tumor growth. [4]

Protocol

Animal Research:[4]
- Collapse
  • Animal Models: Rat bladder tumor model
  • Dosages: 400 μM
  • Administration: Intravesical treatment
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 66 mg/mL (197.38 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 334.37
Formula

C15H18N4O5

CAS No. 50-07-7
Storage 3 years -20°C(in the dark) powder
2 years -80°C(in the dark) in solvent
Synonyms N/A
Smiles CC1=C(C(=O)C2=C(C1=O)N3CC4C(C3(C2COC(=O)N)OC)N4)N

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02199327 Completed Drug: Mitomycin C|Drug: Interferon Alfa-2b Conjunctival Intraepithelial Neoplasia|Corneal Intraepithelial Neoplasia Coordinación de Investigación en Salud Mexico|Instituto Mexicano del Seguro Social|University of Guadalajara May 2014 Phase 4
NCT02015650 Terminated Drug: Cetuximab|Drug: Mitomycin-C/ 5-Fluorouracil Head and Neck Neoplasms Medical University Innsbruck April 2014 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID