IWR-1-endo

For research use only.

Catalog No.S7086

54 publications

IWR-1-endo Chemical Structure

CAS No. 1127442-82-3

IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

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10mM (1mL in DMSO) USD 97 In stock
USD 110 In stock
USD 250 In stock
USD 629 In stock
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Selleck's IWR-1-endo has been cited by 54 publications

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Biological Activity

Description IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.
Targets
Wnt [1]
(L-cells expressing Wnt3A)
180 nM
In vitro

Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HEK293T cells  NIG4R4pHfW6ldHnvckBie3OjeR?= NFzXV3RKdmirYnn0bY9vKG:oIHLleIEu[2G|ZXnuMYRmeGWwZHXueEBk[W6xbnnjZYwhX262MzDwZZRpf2G7IHnuJIh2dWGwIFjFT|I6O1RiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUCuNFI3KM7:TR?= NWjnPVloOjJzOUG1OVc>
mouse L-Wnt-STF cells M4noVmZ2dmO2aX;uJIF{e2G7 NIn6foRKdmirYnn0bY9vKG:oIGTOT3MuOiCrbjDtc5V{\SCOLWfueE1UXEZiY3XscJMh[XO|ZYPz[YQh[XNiZHnzdpVxfGmxbjDv[kBYdnRic3nncoFtcW6pIHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTB;MD6wOVYh|ryP MWKyOFUzPzd7Mh?=
mouse L-Wnt-STF cells MnTHSpVv[3Srb36gZZN{[Xl? M3;6SmlvcGmkaYTpc44hd2ZiVF7LV{0yKGmwIH3veZNmKExvV370MXNVTiClZXzsd{Bie3Onc4Pl[EBieyCmaYPyeZB1cW:wIH;mJHdvfCC|aXfuZYxqdmdiYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkGzNUDPxE1? NE\qemEzPDV{N{e5Ni=>
Escherichia coli BL21 (DE3) cells MmDWSpVv[3Srb36gZZN{[Xl? Mnj5PVAhdWmwcx?= NFXOV4dC[3Srdnn0fUBw\iCQLYTldo1qdnW|IHjlfIFJcXNvdHHn[4VlKGi3bXHuJHRPW0t{IHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGJNOjFiKFTFN{kh[2WubIOgeZNqdmdiYnnveIlvgWyjdHXkJG5CTCtiYYOgd5Vje3S{YYTlJIFnfGW{IEmwJI1qdnNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{NCCHQ{WwQVAvOiEQvF2= M{XkWFIzOjN|M{Kw
human SW480 cells NInWWFVHfW6ldHnvckBie3OjeR?= NF61N4E1OCC2bzC0PEBp MV7Jcohq[mm2aX;uJI9nKHSjbnv5doF{\SCrbjDoeY1idiCVV{S4NEBk\WyuczDhd5Nme3OnZDDhd{Bl\We{YXTheIlwdiCxZjDi[ZRiKGOjdHXubY4h[W[2ZYKgOFAhfG9iNEigbJJ{NCCLQ{WwQVAvOjVizszN NVezcVNCOjN5MEG1NVc>
human SW480 cells MXjGeY5kfGmxbjDhd5NigQ>? NFzNNmUzPCCq M3rKRWlvcGmkaYTpc44hd2ZidHHub5lz[XOnIHnuJIh2dWGwIGPXOFgxKGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDv[kBigGmwMjDh[pRmeiB{NDDodpMh[nliSH;lZ4h{fCCmeXWtZoF{\WRibXX0bI9l M1vFUVI{PzBzNUG3
human HT29 cells NW\jT25VTnWwY4Tpc44h[XO|YYm= NH3UfYgzPCCq M2jQTmlvcGmkaYTpc44hd2ZiV370JJNq\26jbHnu[{BqdiCqdX3hckBJXDJ7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[mW2YT3jZZRmdmmwLX3l[IlifGWmIGTj[k9N\WZidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9tNCCLQ{WwQVI1NjRizszN NXXVO4l6OjR7NUC0PFk>
human HT29 cells M1;OfWZ2dmO2aX;uJIF{e2G7 NEf2XpMzPSEQvF2= MmPrNlQhcA>? NWPn[nl5UW6qaXLpeIlwdiCxZjDXcpQhe2mpbnHsbY5oKGmwIHj1cYFvKEiWMkmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCkZYThMYNifGWwaX6tcYVlcWG2ZXSgWINnN0ynZjD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYYSgNlUhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KHKnbHH0bZZmKHSxIHPvcpRzd2xiaX6gdJJme2WwY3Wgc4YhT1ONLUPi[ZRiKGmwaHnibZRweiCOaVPs MYSyOFk2ODR6OR?=
human DLD1 cells MYPGeY5kfGmxbjDhd5NigQ>? MXGyJIg> MV;JcoR2[3Srb36gc4YhSXirbkKgd5Ri[mmuaYrheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPl[EB1emGwc3PybZB1cW:wIH;mJGF5cW5{IHHmeIVzKDJiaILzJIJ6KFKWLWDDVkBu\XSqb3Su M2\mWVE6OTJ3MUW2
HEK293 cells M3f5WmZ2dmO2aX;uJIF{e2G7 M2LyR2Rqe3CuYXPlcYVvfCCxZjDJW3IuWEJiZoLvcUBCgGmwMjDpckBJTUt{OUOgZ4VtdHNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=> NGfBb5cyQTF{NUG1Oi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
E-cadherin / N-cadherin / Snail / Vimentin; 

PubMed: 26450645     


Western blot analyses showing protein expression patterns of the EMT markers according to IWR-1 dose and exposure time. β-Actin was used as a loading control.

p-Akt / Akt; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the phosphorylation of Akt in a concentration- and time-dependent manner, respectively.

Survivin; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the survivin expression in a concentration- and time-dependent manner, respectively.

26450645

Protocol

Solubility (25°C)

In vitro DMSO 21 mg/mL (51.28 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 409.44
Formula

C25H19N3O3

CAS No. 1127442-82-3
Storage powder
in solvent
Synonyms N/A
Smiles C1C2C=CC1C3C2C(=O)N(C3=O)C4=CC=C(C=C4)C(=O)NC5=CC=CC6=C5N=CC=C6

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID