IWR-1-endo

Catalog No.S7086

IWR-1-endo Chemical Structure

Molecular Weight(MW): 409.44

IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

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Cited by 21 Publications

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Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.
Targets
Wnt [1]
(L-cells expressing Wnt3A)
180 nM
In vitro

Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HEK293T cells  NH;zPXJHfW6ldHnvckBie3OjeR?= MkXqTY5pcWKrdHnvckBw\iCkZYThMYNie2Wrbj3k[ZBmdmSnboSgZ4Fvd26rY3HsJHdvfDNicHH0bJdigSCrbjDoeY1idiCKRVuyPVNVKGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkCyOkDPxE1? MYeyNlE6OTV3Nx?=
mouse L-Wnt-STF cells NX;POppUTnWwY4Tpc44h[XO|YYm= MkG2TY5pcWKrdHnvckBw\iCWTlvTMVIhcW5ibX;1d4UhVC2ZboStV3RHKGOnbHzzJIF{e2W|c3XkJIF{KGSrc4L1dJRqd25ib3[gW451KHOrZ37hcIlv\yCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRTBwMEW2JO69VQ>? MXyyOFUzPzd7Mh?=
mouse L-Wnt-STF cells NF;jUZZHfW6ldHnvckBie3OjeR?= MofWTY5pcWKrdHnvckBw\iCWTlvTMVEhcW5ibX;1d4UhVC2ZboStV3RHKGOnbHzzJIF{e2W|c3XkJIF{KGSrc4L1dJRqd25ib3[gW451KHOrZ37hcIlv\yCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRTBwMUOxJO69VQ>? Mni3NlQ2Ojd5OUK=
Escherichia coli BL21 (DE3) cells M{DldWZ2dmO2aX;uJIF{e2G7 MmDiPVAhdWmwcx?= NYTwOFRXSWO2aY\peJkhd2ZiTj30[ZJucW63czDo[ZhiUGm|LYTh[4dm\CCqdX3hckBVVlONMjDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIDjESVMqKGOnbHzzJJV{cW6pIHLpc5RqdnmuYYTl[EBPSURtIHHzJJN2[nO2cnH0[UBi\nSncjC5NEBucW6|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqeyxiRVO1NF0xNjJizszN MoLmNlIzOzN|MkC=
human SW480 cells MkLpSpVv[3Srb36gZZN{[Xl? NEG1UIg1OCC2bzC0PEBp NFS5cnZKdmirYnn0bY9vKG:oIIThcot6emG|ZTDpckBpfW2jbjDTW|Q5OCClZXzsd{Bie3Onc4Pl[EBieyCmZXfyZYRifGmxbjDv[kBj\XSjIHPheIVvcW5iYX\0[ZIhPDBidH:gOFghcHK|LDDJR|UxRTBwMkWg{txO Mk\vNlM4ODF3MUe=
human SW480 cells MYnGeY5kfGmxbjDhd5NigQ>? NHjVPIszPCCq NVyyWVNpUW6qaXLpeIlwdiCxZjD0ZY5sgXKjc3WgbY4hcHWvYX6gV3c1QDBiY3XscJMh[XO|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJI9nKGG6aX6yJIFnfGW{IEK0JIhzeyCkeTDIc4VkcHO2IHT5[U1j[XOnZDDt[ZRpd2R? M2rkT|I{PzBzNUG3
human HT29 cells M4TxRmZ2dmO2aX;uJIF{e2G7 MlnnNlQhcA>? NX\Sd|R4UW6qaXLpeIlwdiCxZjDXcpQhe2mpbnHsbY5oKGmwIHj1cYFvKEiWMkmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCkZYThMYNifGWwaX6tcYVlcWG2ZXSgWINnN0ynZjD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KHKnbHH0bZZmKHSxIHPvcpRzd2xuIFnDOVA:OjRwNDFOwG0> MkC2NlQ6PTB2OEm=
human HT29 cells MUDGeY5kfGmxbjDhd5NigQ>? MojNNlUh|ryP NGXZOXozPCCq MoX1TY5pcWKrdHnvckBw\iCZboSgd4lodmGuaX7nJIlvKGi3bXHuJGhVOjliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBj\XSjLXPheIVvcW5vbXXkbYF1\WRiVHPmM2xm\iC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkh[XRiMkWgeW0h[W[2ZYKgNlQhcHK|IHL5JIR2[WxibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5JJJmdGG2aY\lJJRwKGOxboTyc4whcW5icILld4Vv[2Vib3[gS3NMNTOkZYThJIlvcGmkaYTvdkBNcUOu NGqzRYgzPDl3MES4PS=>
human DLD1 cells M{PzV2Z2dmO2aX;uJIF{e2G7 MV2yJIg> MV3JcoR2[3Srb36gc4YhSXirbkKgd5Ri[mmuaYrheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPl[EB1emGwc3PybZB1cW:wIH;mJGF5cW5{IHHmeIVzKDJiaILzJIJ6KFKWLWDDVkBu\XSqb3Su MlrCNVkyOjVzNU[=
HEK293 cells Mlj3SpVv[3Srb36gZZN{[Xl? MVPEbZNxdGGlZX3lcpQhd2ZiSWfSMXBDKG[{b32gRZhqdjJiaX6gTGVMOjl|IHPlcIx{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S= MUCxPVEzPTF3Nh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
E-cadherin / N-cadherin / Snail / Vimentin; 

PubMed: 26450645     


Western blot analyses showing protein expression patterns of the EMT markers according to IWR-1 dose and exposure time. β-Actin was used as a loading control.

p-Akt / Akt; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the phosphorylation of Akt in a concentration- and time-dependent manner, respectively.

Survivin; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the survivin expression in a concentration- and time-dependent manner, respectively.

26450645

Protocol

Solubility (25°C)

In vitro DMSO 21 mg/mL (51.28 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 409.44
Formula

C25H19N3O3

CAS No. 1127442-82-3
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID