IWR-1-endo

Catalog No.S7086

IWR-1-endo Chemical Structure

Molecular Weight(MW): 409.44

IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

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USD 250 In stock
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Cited by 8 Publications

3 Customer Reviews

  • Effect of IWR-1 treatment on total β-catenin levels and adipogenesis in high-passage shSCR and shTCF7L2 cells. Cells were treated with IWR-1 (10 μmol/L) every other day for 6 days during differentiation. Western blot demonstrates the effect of IWR-1 on total β-catenin protein levels at the end of adipogenesis (each lane represents a single independent adipogenesis experiment, n = 4 for each treatment).

    Diabetes, 2018, 67(4):554-568. IWR-1-endo purchased from Selleck.

    Dose effect of IWR-1-endo, PRI-724, and ICG-001 on FAS and CD36 protein levels measured by immunoblots.

    Biochim Biophys Acta Mol Cell Biol Lipids, 2018, 1863(8):834-843. IWR-1-endo purchased from Selleck.

  • (F) UNC119 influences the activity of Wnt/β-catenin signaling. β-catenin inhibitor IWR-1-endo significantly inhibits SKHep1 proliferation.

    Am J Cancer Res, 2015, 5(10):3123-3134.. IWR-1-endo purchased from Selleck.

Purity & Quality Control

Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.
Targets
Wnt [1]
(L-cells expressing Wnt3A)
180 nM
In vitro

Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HEK293T cells  NGW1OVNHfW6ldHnvckBie3OjeR?= MkG0TY5pcWKrdHnvckBw\iCkZYThMYNie2Wrbj3k[ZBmdmSnboSgZ4Fvd26rY3HsJHdvfDNicHH0bJdigSCrbjDoeY1idiCKRVuyPVNVKGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkCyOkDPxE1? NWrjSZFQOjJzOUG1OVc>
mouse L-Wnt-STF cells NEKzUZZHfW6ldHnvckBie3OjeR?= MmLQTY5pcWKrdHnvckBw\iCWTlvTMVIhcW5ibX;1d4UhVC2ZboStV3RHKGOnbHzzJIF{e2W|c3XkJIF{KGSrc4L1dJRqd25ib3[gW451KHOrZ37hcIlv\yCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRTBwMEW2JO69VQ>? MonSNlQ2Ojd5OUK=
mouse L-Wnt-STF cells NUTBSnBvTnWwY4Tpc44h[XO|YYm= Ml7STY5pcWKrdHnvckBw\iCWTlvTMVEhcW5ibX;1d4UhVC2ZboStV3RHKGOnbHzzJIF{e2W|c3XkJIF{KGSrc4L1dJRqd25ib3[gW451KHOrZ37hcIlv\yCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRTBwMUOxJO69VQ>? M3vQRlI1PTJ5N{my
Escherichia coli BL21 (DE3) cells NHfkZYNHfW6ldHnvckBie3OjeR?= NVv3[45KQTBibXnudy=> MWPBZ5Rqfmm2eTDv[kBPNXSncn3pcpV{KGineHHIbZMufGGpZ3XkJIh2dWGwIGTOV2szKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEhMESHMzmgZ4VtdHNidYPpcoch[mmxdHnufYxifGWmIF7BSEsh[XNic4Xid5Rz[XSnIHHmeIVzKDlyIH3pcpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nzMEBGSzVyPUCuNkDPxE1? NUH2XohDOjJ{M{OzNlA>
human SW480 cells NH;5V4FHfW6ldHnvckBie3OjeR?= MmHMOFAhfG9iNEigbC=> MUnJcohq[mm2aX;uJI9nKHSjbnv5doF{\SCrbjDoeY1idiCVV{S4NEBk\WyuczDhd5Nme3OnZDDhd{Bl\We{YXTheIlwdiCxZjDi[ZRiKGOjdHXubY4h[W[2ZYKgOFAhfG9iNEigbJJ{NCCLQ{WwQVAvOjVizszN MmCzNlM4ODF3MUe=
human SW480 cells MWPGeY5kfGmxbjDhd5NigQ>? M2nwVVI1KGh? NUC0NJlnUW6qaXLpeIlwdiCxZjD0ZY5sgXKjc3WgbY4hcHWvYX6gV3c1QDBiY3XscJMh[XO|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJI9nKGG6aX6yJIFnfGW{IEK0JIhzeyCkeTDIc4VkcHO2IHT5[U1j[XOnZDDt[ZRpd2R? M4PK[lI{PzBzNUG3
human HT29 cells M3\mXWZ2dmO2aX;uJIF{e2G7 NXfKN41LOjRiaB?= MVvJcohq[mm2aX;uJI9nKFewdDDzbYdv[WyrbnegbY4hcHWvYX6gTHQzQSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIJmfGFvY3H0[Y5qdi2vZXTpZZRm\CCWY3[vUIVnKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBi\nSncjCyOEBpenNiYomg[JVidCCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmgdoVt[XSrdnWgeI8h[2:wdILvcEwhUUN3ME2yOE41KM7:TR?= MmHENlQ6PTB2OEm=
human HT29 cells NU\ydIs5TnWwY4Tpc44h[XO|YYm= NHTheHgzPSEQvF2= MVWyOEBp M3vqSGlvcGmkaYTpc44hd2ZiV370JJNq\26jbHnu[{BqdiCqdX3hckBJXDJ7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[mW2YT3jZZRmdmmwLX3l[IlifGWmIGTj[k9N\WZidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGG2IEK1JJVOKGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9tKGmwIIDy[ZNmdmOnIH;mJGdUUy1|YnX0ZUBqdmirYnn0c5IhVGmFbB?= MUSyOFk2ODR6OR?=
human DLD1 cells NHjOdZpHfW6ldHnvckBie3OjeR?= M3HLcFIhcA>? NYHyVY9MUW6mdXP0bY9vKG:oIFH4bY4zKHO2YXLpcIl7[XSrb36gbY4hcHWvYX6gSGxFOSClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\WRidILhcpNkemmydHnvckBw\iCDeHnuNkBi\nSncjCyJIhzeyCkeTDSWE1RS1JibXX0bI9lNg>? MXWxPVEzPTF3Nh?=
HEK293 cells NHGzcppHfW6ldHnvckBie3OjeR?= NFf2fWVFcXOybHHj[Y1mdnRib3[gTXdTNVCEIH\yc40hSXirbkKgbY4hUEWNMkmzJINmdGy|IHL5JHdme3Sncn6gZoxwfCCvZYToc4Q> MojGNVkyOjVzNU[=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
E-cadherin / N-cadherin / Snail / Vimentin; 

PubMed: 26450645     


Western blot analyses showing protein expression patterns of the EMT markers according to IWR-1 dose and exposure time. β-Actin was used as a loading control.

p-Akt / Akt; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the phosphorylation of Akt in a concentration- and time-dependent manner, respectively.

Survivin; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the survivin expression in a concentration- and time-dependent manner, respectively.

26450645

Protocol

Solubility (25°C)

In vitro DMSO 21 mg/mL (51.28 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 409.44
Formula

C25H19N3O3

CAS No. 1127442-82-3
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Wnt/beta-catenin Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID