IWR-1-endo

Catalog No.S7086

IWR-1-endo Chemical Structure

Molecular Weight(MW): 409.44

IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

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Cited by 24 Publications

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Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.
Targets
Wnt [1]
(L-cells expressing Wnt3A)
180 nM
In vitro

Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HEK293T cells  MWLGeY5kfGmxbjDhd5NigQ>? MoTUTY5pcWKrdHnvckBw\iCkZYThMYNie2Wrbj3k[ZBmdmSnboSgZ4Fvd26rY3HsJHdvfDNicHH0bJdigSCrbjDoeY1idiCKRVuyPVNVKGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkCyOkDPxE1? M4D5fFIzOTlzNUW3
mouse L-Wnt-STF cells MlvMSpVv[3Srb36gZZN{[Xl? M2fpWWlvcGmkaYTpc44hd2ZiVF7LV{0zKGmwIH3veZNmKExvV370MXNVTiClZXzsd{Bie3Onc4Pl[EBieyCmaYPyeZB1cW:wIH;mJHdvfCC|aXfuZYxqdmdiYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkC1OkDPxE1? MUOyOFUzPzd7Mh?=
mouse L-Wnt-STF cells MkGwSpVv[3Srb36gZZN{[Xl? M4nYW2lvcGmkaYTpc44hd2ZiVF7LV{0yKGmwIH3veZNmKExvV370MXNVTiClZXzsd{Bie3Onc4Pl[EBieyCmaYPyeZB1cW:wIH;mJHdvfCC|aXfuZYxqdmdiYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkGzNUDPxE1? Mkn1NlQ2Ojd5OUK=
Escherichia coli BL21 (DE3) cells M1jsUmZ2dmO2aX;uJIF{e2G7 MmX2PVAhdWmwcx?= MUPBZ5Rqfmm2eTDv[kBPNXSncn3pcpV{KGineHHIbZMufGGpZ3XkJIh2dWGwIGTOV2szKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEhMESHMzmgZ4VtdHNidYPpcoch[mmxdHnufYxifGWmIF7BSEsh[XNic4Xid5Rz[XSnIHHmeIVzKDlyIH3pcpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nzMEBGSzVyPUCuNkDPxE1? Ml7lNlIzOzN|MkC=
human SW480 cells NYfzc5lFTnWwY4Tpc44h[XO|YYm= M4D5b|QxKHSxIES4JIg> NIfEeZVKdmirYnn0bY9vKG:oIIThcot6emG|ZTDpckBpfW2jbjDTW|Q5OCClZXzsd{Bie3Onc4Pl[EBieyCmZXfyZYRifGmxbjDv[kBj\XSjIHPheIVvcW5iYX\0[ZIhPDBidH:gOFghcHK|LDDJR|UxRTBwMkWg{txO M1L6ZlI{PzBzNUG3
human SW480 cells MWnGeY5kfGmxbjDhd5NigQ>? M1na[VI1KGh? M{TLTGlvcGmkaYTpc44hd2ZidHHub5lz[XOnIHnuJIh2dWGwIGPXOFgxKGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDv[kBigGmwMjDh[pRmeiB{NDDodpMh[nliSH;lZ4h{fCCmeXWtZoF{\WRibXX0bI9l MmKxNlM4ODF3MUe=
human HT29 cells MmfOSpVv[3Srb36gZZN{[Xl? M{\XTFI1KGh? M4LUeWlvcGmkaYTpc44hd2ZiV370JJNq\26jbHnu[{BqdiCqdX3hckBJXDJ7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[mW2YT3jZZRmdmmwLX3l[IlifGWmIGTj[k9N\WZidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9tNCCLQ{WwQVI1NjRizszN MWGyOFk2ODR6OR?=
human HT29 cells MnjCSpVv[3Srb36gZZN{[Xl? MUWyOUDPxE1? Mm\ONlQhcA>? MmfMTY5pcWKrdHnvckBw\iCZboSgd4lodmGuaX7nJIlvKGi3bXHuJGhVOjliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBj\XSjLXPheIVvcW5vbXXkbYF1\WRiVHPmM2xm\iC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkh[XRiMkWgeW0h[W[2ZYKgNlQhcHK|IHL5JIR2[WxibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5JJJmdGG2aY\lJJRwKGOxboTyc4whcW5icILld4Vv[2Vib3[gS3NMNTOkZYThJIlvcGmkaYTvdkBNcUOu M3e3VFI1QTVyNEi5
human DLD1 cells Mn\vSpVv[3Srb36gZZN{[Xl? NHHBdGQzKGh? MWnJcoR2[3Srb36gc4YhSXirbkKgd5Ri[mmuaYrheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPl[EB1emGwc3PybZB1cW:wIH;mJGF5cW5{IHHmeIVzKDJiaILzJIJ6KFKWLWDDVkBu\XSqb3Su MXexPVEzPTF3Nh?=
HEK293 cells MYnGeY5kfGmxbjDhd5NigQ>? M{m1U2Rqe3CuYXPlcYVvfCCxZjDJW3IuWEJiZoLvcUBCgGmwMjDpckBJTUt{OUOgZ4VtdHNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=> NFW4UHAyQTF{NUG1Oi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
E-cadherin / N-cadherin / Snail / Vimentin; 

PubMed: 26450645     


Western blot analyses showing protein expression patterns of the EMT markers according to IWR-1 dose and exposure time. β-Actin was used as a loading control.

p-Akt / Akt; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the phosphorylation of Akt in a concentration- and time-dependent manner, respectively.

Survivin; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the survivin expression in a concentration- and time-dependent manner, respectively.

26450645

Protocol

Solubility (25°C)

In vitro DMSO 21 mg/mL (51.28 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 409.44
Formula

C25H19N3O3

CAS No. 1127442-82-3
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID