IWR-1-endo

For research use only.

Catalog No.S7086

38 publications

IWR-1-endo Chemical Structure

Molecular Weight(MW): 409.44

IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

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Selleck's IWR-1-endo has been cited by 38 publications

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Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description IWR-1-endo is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.
Targets
Wnt [1]
(L-cells expressing Wnt3A)
180 nM
In vitro

Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HEK293T cells  MYDGeY5kfGmxbjDhd5NigQ>? M37TcmlvcGmkaYTpc44hd2ZiYnX0ZU1k[XOnaX6t[IVx\W6mZX70JINidm:waXPhcEBYdnR|IIDheIh4[XliaX6gbJVu[W5iSFXLNlk{XCClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTB;MD6wNlYh|ryP MnXmNlIyQTF3NUe=
mouse L-Wnt-STF cells MV;GeY5kfGmxbjDhd5NigQ>? NVXIWYkxUW6qaXLpeIlwdiCxZjDUUmtUNTJiaX6gcY92e2ViTD3XcpQuW1SIIHPlcIx{KGG|c3Xzd4VlKGG|IHTpd5J2eHSrb36gc4YhX262IIPp[45idGmwZzDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUCuNFU3KM7:TR?= Ml\UNlQ2Ojd5OUK=
mouse L-Wnt-STF cells MkXTSpVv[3Srb36gZZN{[Xl? MXfJcohq[mm2aX;uJI9nKFSQS2OtNUBqdiCvb4Xz[UBNNVewdD3TWGYh[2WubIOgZZN{\XO|ZXSgZZMh\Gm|coXweIlwdiCxZjDXcpQhe2mpbnHsbY5oKGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:OC5zM{Gg{txO NILuUpczPDV{N{e5Ni=>
Escherichia coli BL21 (DE3) cells NEjRZ3NHfW6ldHnvckBie3OjeR?= MnPVPVAhdWmwcx?= M1nqe2FkfGm4aYT5JI9nKE5vdHXycYlvfXNiaHX4ZWhqey22YXfn[YQhcHWvYX6gWG5UUzJiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSBqRFWzLUBk\WyuczD1d4lv\yCkaX;0bY56dGG2ZXSgUmFFMyCjczDzeYJ{fHKjdHWgZYZ1\XJiOUCgcYlveyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZMtKEWFNUC9NE4zKM7:TR?= NUW0VXZ5OjJ{M{OzNlA>
human SW480 cells MoHuSpVv[3Srb36gZZN{[Xl? NX7GTYlqPDBidH:gOFghcA>? M3TqXmlvcGmkaYTpc44hd2ZidHHub5lz[XOnIHnuJIh2dWGwIGPXOFgxKGOnbHzzJIF{e2W|c3XkJIF{KGSnZ4Lh[IF1cW:wIH;mJIJmfGFiY3H0[Y5qdiCjZoTldkA1OCC2bzC0PEBpenNuIFnDOVA:OC5{NTFOwG0> MUCyN|cxOTVzNx?=
human SW480 cells MoHWSpVv[3Srb36gZZN{[Xl? NX\qcY1EOjRiaB?= MULJcohq[mm2aX;uJI9nKHSjbnv5doF{\SCrbjDoeY1idiCVV{S4NEBk\WyuczDhd5Nme3OnZDDhd{Bi[2O3bYXsZZRqd25ib3[gZZhqdjJiYX\0[ZIhOjRiaILzJIJ6KEixZXPod5Qh\HmnLXLhd4VlKG2ndHjv[C=> M4LsfVI{PzBzNUG3
human HT29 cells NEPF[W1HfW6ldHnvckBie3OjeR?= MmD2NlQhcA>? NWL1bIRXUW6qaXLpeIlwdiCxZjDXcpQhe2mpbnHsbY5oKGmwIHj1cYFvKEiWMkmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCkZYThMYNifGWwaX6tcYVlcWG2ZXSgWINnN0ynZjD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KHKnbHH0bZZmKHSxIHPvcpRzd2xuIFnDOVA:OjRwNDFOwG0> MVOyOFk2ODR6OR?=
human HT29 cells MoHVSpVv[3Srb36gZZN{[Xl? MYSyOUDPxE1? NIfuVlgzPCCq M{DjOWlvcGmkaYTpc44hd2ZiV370JJNq\26jbHnu[{BqdiCqdX3hckBJXDJ7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[mW2YT3jZZRmdmmwLX3l[IlifGWmIGTj[k9N\WZidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGG2IEK1JJVOKGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9tKGmwIIDy[ZNmdmOnIH;mJGdUUy1|YnX0ZUBqdmirYnn0c5IhVGmFbB?= NEHKbYwzPDl3MES4PS=>
human DLD1 cells NWrJT3BCTnWwY4Tpc44h[XO|YYm= NYjITHh6OiCq MXLJcoR2[3Srb36gc4YhSXirbkKgd5Ri[mmuaYrheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPl[EB1emGwc3PybZB1cW:wIH;mJGF5cW5{IHHmeIVzKDJiaILzJIJ6KFKWLWDDVkBu\XSqb3Su M37EN|E6OTJ3MUW2
HEK293 cells MlP0SpVv[3Srb36gZZN{[Xl? NYXJWndxTGm|cHzhZ4Vu\W62IH;mJGlYWi2SQjDmdo9uKEG6aX6yJIlvKEiHS{K5N{Bk\WyuczDifUBY\XO2ZYLuJIJtd3RibXX0bI9l M1myflE6OTJ3MUW2

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
E-cadherin / N-cadherin / Snail / Vimentin; 

PubMed: 26450645     


Western blot analyses showing protein expression patterns of the EMT markers according to IWR-1 dose and exposure time. β-Actin was used as a loading control.

p-Akt / Akt; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the phosphorylation of Akt in a concentration- and time-dependent manner, respectively.

Survivin; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the survivin expression in a concentration- and time-dependent manner, respectively.

26450645

Protocol

Solubility (25°C)

In vitro DMSO 21 mg/mL (51.28 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 409.44
Formula

C25H19N3O3

CAS No. 1127442-82-3
Storage powder
in solvent
Synonyms N/A
Smiles CC12C3CC(C=C3)C1C(=O)N(C2=O)C4=CC=C(C=C4)C(=O)NC5=CC=CC6=C5N=CC=C6

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID