IWR-1-endo

For research use only.

Catalog No.S7086 Synonyms: endo-IWR 1

69 publications

IWR-1-endo Chemical Structure

CAS No. 1127442-82-3

IWR-1-endo (endo-IWR 1) is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

Selleck's IWR-1-endo has been cited by 69 publications

Purity & Quality Control

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Biological Activity

Description IWR-1-endo (endo-IWR 1) is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.
Targets
Wnt [1]
(L-cells expressing Wnt3A)
180 nM
In vitro

Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BL21 (DE3) MWHGeY5kfGmxbjDhd5NigQ>? M2DreVkxKG2rboO= M4jhfmFkfGm4aYT5JI9nKE5vdHXycYlvfXNiaHX4ZWhqey22YXfn[YQhcHWvYX6gWG5UUzJiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSBqRFWzLUBk\WyuczD1d4lv\yCkaX;0bY56dGG2ZXSgUmFFMyCjczDzeYJ{fHKjdHWgZYZ1\XJiOUCgcYlveyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZMtKEWFNUCgQUAxNjJizszNMi=> NVLjSXp2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyN|M{OjBpPkKyNlM{OzJyPD;hQi=>
SW480 NETqNm5HfW6ldHnvckBie3OjeR?= M{HEOlI1KGi{cx?= MX\Jcohq[mm2aX;uJI9nKHSjbnv5doF{\SCrbjDoeY1idiCVV{S4NEBk\WyuczDhd5Nme3OnZDDhd{Bi[2O3bYXsZZRqd25ib3[gZZhqdjJiYX\0[ZIhOjRiaILzJIJ6KEixZXPod5Qh\HmnLXLhd4VlKG2ndHjv[EwhTUN3MDC9JFIvPSEQvF2u MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyMUWxO{c,OjN5MEG1NVc9N2F-
HEK293T NFv5e4RHfW6ldHnvckBie3OjeR?= NH\sSXVKdmirYnn0bY9vKG:oIHLleIEu[2G|ZXnuMYRmeGWwZHXueEBk[W6xbnnjZYwhX262MzDwZZRpf2G7IHnuJIh2dWGwIFjFT|I6O1RiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyIE2gNE4xOjZizszNMi=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF7MUW1O{c,OjJzOUG1OVc9N2F-
SW480 NVnlW3p2TnWwY4Tpc44h[XO|YYm= NEHjepA1OCC2bzC0PEBpenN? NWfCfIFwUW6qaXLpeIlwdiCxZjD0ZY5sgXKjc3WgbY4hcHWvYX6gV3c1QDBiY3XscJMh[XO|ZYPz[YQh[XNiZHXndoFl[XSrb36gc4Yh[mW2YTDjZZRmdmmwIHHmeIVzKDRyIITvJFQ5KGi{czygTWM2OCB;IECuNlUh|ryPLh?= NUXXZ5ZMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFE2OTdpPkKzO|AyPTF5PD;hQi=>
L-Wnt-STF MUfGeY5kfGmxbjDhd5NigQ>? M{DxWFI1KGi{cx?= M4jMfmlvcGmkaYTpc44hd2ZiVF7LV{0zKGmwIH3veZNmKExvV370MXNVTiClZXzsd{Bie3Onc4Pl[EBieyCmaYPyeZB1cW:wIH;mJHdvfCC|aXfuZYxqdmdiYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuNFU3KM7:TT6= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{N{e5Nkc,OjR3Mke3PVI9N2F-
L-Wnt-STF M3;KPGZ2dmO2aX;uJIF{e2G7 M3nuO|I1KGi{cx?= MWHJcohq[mm2aX;uJI9nKFSQS2OtNUBqdiCvb4Xz[UBNNVewdD3TWGYh[2WubIOgZZN{\XO|ZXSgZZMh\Gm|coXweIlwdiCxZjDXcpQhe2mpbnHsbY5oKGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVAhRSByLkGzNUDPxE1w MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{N{e5Nkc,OjR3Mke3PVI9N2F-
HT29 MmG5SpVv[3Srb36gZZN{[Xl? NUW0T4x6OjRiaILz MoXBTY5pcWKrdHnvckBw\iCZboSgd4lodmGuaX7nJIlvKGi3bXHuJGhVOjliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBj\XSjLXPheIVvcW5vbXXkbYF1\WRiVHPmM2xm\iC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkh[W[2ZYKgNlQhcHK|IHL5JIR2[WxibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5JJJmdGG2aY\lJJRwKGOxboTyc4wtKEmFNUCgQUAzPC52IN88UU4> NUHI[4JlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OVA1QDlpPkK0PVUxPDh7PD;hQi=>
DLD1 MnnJSpVv[3Srb36gZZN{[Xl? MkHDNVAhfU1? M{jwTmlv\HWldHnvckBw\iCDeHnuNkBqdiCqdX3hckBFVERzIHPlcIx{KG:4ZYLlfJBz\XO|aX7nJGlYWi2LUzDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCZboSgdIF1cHejeTDhZ5Rqfmm2eTDheEAyOCC3TTDifUBUXEZicnXwc5J1\XJiYYPzZZkhdWW2aH;k NEXaUWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGyOVE2Pid-MUmxNlUyPTZ:L3G+
HEK293 NYfzNJEyTnWwY4Tpc44h[XO|YYm= M4LSfWRqe3CuYXPlcYVvfCCxZjDJW3IuWEJiZoLvcUBCgGmwMjDpckBJTUt{OUOgZ4VtdHNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=> NGCzVoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGyOVE2Pid-MUmxNlUyPTZ:L3G+
DLD1 NH:zb49HfW6ldHnvckBie3OjeR?= NIr1N5lKdmirYnn0bY9vKG:oIFH4bY4zKHC{b4TlbY4h\GWpcnHkZZRqd25iaX6gbJVu[W5iRFzENUBk\WyuczDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? NFrodWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGyOVE2Pid-MUmxNlUyPTZ:L3G+
DLD1 NXXIRWpKTnWwY4Tpc44h[XO|YYm= MkS1TY5lfWO2aX;uJI9nKEG6aX6yJIFk[3WvdXzheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKFSqckSxJJBpd3OyaH;yfYxifGWmIHLleIEu[2G|ZXnuJIxmfmWuczDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? NGLjcWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGyOVE2Pid-MUmxNlUyPTZ:L3G+
DLD1 M2HydmZ2dmO2aX;uJIF{e2G7 MlfaTY5lfWO2aX;uJI9nKEG6aX6yJIFk[3WvdXzheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKFOnckO3JJBpd3OyaH;yfYxifGWmIHLleIEu[2G|ZXnuJIxmfmWuczDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? NVXFd4x6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNlUyPTZpPkG5NVI2OTV4PD;hQi=>
DLD1 MWDGeY5kfGmxbjDhd5NigQ>? NVq2Z5NqTGWlcnXhd4UhcW5iYnX0ZU1k[XOnaX6gZYNkfW23bHH0bY9vKGmwIHj1cYFvKESOREGgZ4VtdHNiZYjwdoV{e2mwZzDpckBCWENibYX0ZY51 M3i3SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK1NVU3Lz5zOUGyOVE2PjxxYU6=
DLD1 NH\vToRHfW6ldHnvckBie3OjeR?= MUmyJIhzew>? MlPLTY5lfWO2aX;uJI9nKEG6aX6yJJN1[WKrbHn6ZZRqd25iaX6gbJVu[W5iRFzENUBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[YQhfHKjboPjdolxfGmxbjDv[kBCgGmwMjDh[pRmeiB{IHjyd{BjgSCUVD3QR3IhdWW2aH;k MoDwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzMkWxOVYoRjF7MUK1NVU3RC:jPh?=
DLD1 MYfGeY5kfGmxbjDhd5NigQ>? MYLJcoR2[3Srb36gc4YhSXirbkKgZYNkfW23bHH0bY9vKGmwIHj1cYFvKESOREGgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4h\nKnZTDi[ZRiNWOjc3XpckBt\X[nbIOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| M1\qdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK1NVU3Lz5zOUGyOVE2PjxxYU6=
DLD1 NUDKenRiTnWwY4Tpc44h[XO|YYm= NXrNdJlOUW6mdXP0bY9vKG:oIFH4bY4zKHO2YXLpcIl7[XSrb36gbY4hcHWvYX6gSGxFOSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHdvfC:kZYThMYNie2WrbjDwZZRpf2G7 NU\sZlFDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNlUyPTZpPkG5NVI2OTV4PD;hQi=>
DLD1 MnfTSpVv[3Srb36gZZN{[Xl? NFPjNYxKdmS3Y4Tpc44hd2ZiQYjpclIh[WOldX31cIF1cW:wIHnuJIh2dWGwIFTMSFEh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iU3XyN|MheGixc4Doc5J6dGG2ZXSgZoV1[S2lYYPlbY4hdGW4ZXzzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= NYH4V4Y3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNlUyPTZpPkG5NVI2OTV4PD;hQi=>
DLD1 MVrGeY5kfGmxbjDhd5NigQ>? MnLHTY5lfWO2aX;uJI9nKEG6aX6yJIFk[3WvdXzheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? M1\rPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK1NVU3Lz5zOUGyOVE2PjxxYU6=
DLD1 Ml\kSpVv[3Srb36gZZN{[Xl? NFfaT3YyKHSxIEKwJJVO MkH2NlQhcHK| Mk\aTY5pcWKrdHnvckBw\iC2YX7rfZJie2ViaX6gbJVu[W5iRFzENUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGTDSk1l\XCnbnTlcpQhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIF1KDFidH:gNlAhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 NWPzfY1mRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1Nlc4QTJpPkK0OVI4Pzl{PD;hQi=>
DLD1 MV3DfZRwfG:6aXPpeJkh[XO|YYm= M1e0TlEhfG9iMkCgeW0> MYmxNEBl[Xm| NYnlZ|RlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTEyGMTDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDheEAyKHSxIEKwJJVOKG2nYYP1doVlKG:wIHThfUAyOCCkeTDjdpl{fGGuII\pc4xmfCC|dHHpcolv\w>? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{N{e5Nkc,OjR3Mke3PVI9N2F-
HT29 NVjhVnYzTnWwY4Tpc44h[XO|YYm= MWCyOUB2VQ>? MUKyOEBpenN? M2LKXmlvcGmkaYTpc44hd2ZiV370JJNq\26jbHnu[{BqdiCqdX3hckBJXDJ7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[mW2YT3jZZRmdmmwLX3l[IlifGWmIGTj[k9N\WZidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGG2IEK1JJVOKGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9t NFXTW449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm1NFQ5QSd-MkS5OVA1QDl:L3G+
HT29 NXv6XIJiTnWwY4Tpc44h[XO|YYm= MlnyNlUhfU1? MlnRNlQhcHK| NWfwTI1LUW6qaXLpeIlwdiCxZjDXcpQhe2mpbnHsbY5oKGmwIHj1cYFvKEiWMkmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCkZYThMYNifGWwaX6tcYVlcWG2ZXSgWINnN0ynZjD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYYSgNlUhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KHKnbHH0bZZmKHSxIHPvcpRzd2xiaX6gdJJme2WwY3Wgc4YhT1ONLUPi[ZRiKGmwaHnibZRweiCOaVPs NXvBPXhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OVA1QDlpPkK0PVUxPDh7PD;hQi=>
HT29 MonRSpVv[3Srb36gZZN{[Xl? MXSyOUB2VQ>? NWe2XJVXOjRiaILz NUTodW9tUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDpckBpfW2jbjDIWFI6KGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHH4bY4zKG2UTlGg[ZhxemW|c3nvckBifCB{NTD1UUBi\nSncjCyOEBpenNiYomgdZVidnSrdHH0bZZmKHKnYXyteIlu\SCSQ2KgZZN{[Xl? Mmn2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NUC0PFkoRjJ2OUWwOFg6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
E-cadherin / N-cadherin / Snail / Vimentin; 

PubMed: 26450645     


Western blot analyses showing protein expression patterns of the EMT markers according to IWR-1 dose and exposure time. β-Actin was used as a loading control.

p-Akt / Akt; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the phosphorylation of Akt in a concentration- and time-dependent manner, respectively.

Survivin; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the survivin expression in a concentration- and time-dependent manner, respectively.

26450645

Protocol

Solubility (25°C)

In vitro DMSO 21 mg/mL (51.28 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 409.44
Formula

C25H19N3O3

CAS No. 1127442-82-3
Storage powder
in solvent
Synonyms endo-IWR 1
Smiles C1C2C=CC1C3C2C(=O)N(C3=O)C4=CC=C(C=C4)C(=O)NC5=CC=CC6=C5N=CC=C6

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID