IWR-1-endo

For research use only.

Catalog No.S7086 Synonyms: endo-IWR 1

64 publications

IWR-1-endo Chemical Structure

CAS No. 1127442-82-3

IWR-1-endo (endo-IWR 1) is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

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Selleck's IWR-1-endo has been cited by 64 publications

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Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description IWR-1-endo (endo-IWR 1) is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.
Targets
Wnt [1]
(L-cells expressing Wnt3A)
180 nM
In vitro

Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BL21 (DE3) MX;GeY5kfGmxbjDhd5NigQ>? NH3i[GE6OCCvaX7z NXPkN|NVSWO2aY\peJkhd2ZiTj30[ZJucW63czDo[ZhiUGm|LYTh[4dm\CCqdX3hckBVVlONMjDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIDjESVMqKGOnbHzzJJV{cW6pIHLpc5RqdnmuYYTl[EBPSURtIHHzJJN2[nO2cnH0[UBi\nSncjC5NEBucW6|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqeyxiRVO1NEA:KDBwMjFOwG0v NVnrSpBoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyN|M{OjBpPkKyNlM{OzJyPD;hQi=>
SW480 NEG2U2RHfW6ldHnvckBie3OjeR?= Ml63NlQhcHK| NIrmSWdKdmirYnn0bY9vKG:oIIThcot6emG|ZTDpckBpfW2jbjDTW|Q5OCClZXzsd{Bie3Onc4Pl[EBieyCjY3P1cZVt[XSrb36gc4Yh[XirbkKgZYZ1\XJiMkSgbJJ{KGK7IFjv[YNpe3RiZInlMYJie2WmIH3leIhw\CxiRVO1NEA:KDJwNTFOwG0v NYT1e3pORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFE2OTdpPkKzO|AyPTF5PD;hQi=>
HEK293T NHf2PZRHfW6ldHnvckBie3OjeR?= NU\jfpN7UW6qaXLpeIlwdiCxZjDi[ZRiNWOjc3Xpck1l\XCnbnTlcpQh[2Gwb37pZ4FtKFewdEOgdIF1cHejeTDpckBpfW2jbjDISWszQTOWIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvODJ4IN88UU4> NGXt[oc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkG5NVU2Pyd-MkKxPVE2PTd:L3G+
SW480 Ml7mSpVv[3Srb36gZZN{[Xl? NXjCO2JuPDBidH:gOFghcHK| NVHP[Gl{UW6qaXLpeIlwdiCxZjD0ZY5sgXKjc3WgbY4hcHWvYX6gV3c1QDBiY3XscJMh[XO|ZYPz[YQh[XNiZHXndoFl[XSrb36gc4Yh[mW2YTDjZZRmdmmwIHHmeIVzKDRyIITvJFQ5KGi{czygTWM2OCB;IECuNlUh|ryPLh?= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyMUWxO{c,OjN5MEG1NVc9N2F-
L-Wnt-STF MlnQSpVv[3Srb36gZZN{[Xl? M3riNVI1KGi{cx?= MnXmTY5pcWKrdHnvckBw\iCWTlvTMVIhcW5ibX;1d4UhVC2ZboStV3RHKGOnbHzzJIF{e2W|c3XkJIF{KGSrc4L1dJRqd25ib3[gW451KHOrZ37hcIlv\yCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxKD1iMD6wOVYh|ryPLh?= MkXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3Mke3PVIoRjJ2NUK3O|kzRC:jPh?=
L-Wnt-STF NIPjVZZHfW6ldHnvckBie3OjeR?= NX;IdotEOjRiaILz MYLJcohq[mm2aX;uJI9nKFSQS2OtNUBqdiCvb4Xz[UBNNVewdD3TWGYh[2WubIOgZZN{\XO|ZXSgZZMh\Gm|coXweIlwdiCxZjDXcpQhe2mpbnHsbY5oKGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVAhRSByLkGzNUDPxE1w NUXYcIF1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1Nlc4QTJpPkK0OVI4Pzl{PD;hQi=>
HT29 NX3QVG1wTnWwY4Tpc44h[XO|YYm= MnTZNlQhcHK| MX\Jcohq[mm2aX;uJI9nKFewdDDzbYdv[WyrbnegbY4hcHWvYX6gTHQzQSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIJmfGFvY3H0[Y5qdi2vZXTpZZRm\CCWY3[vUIVnKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBi\nSncjCyOEBpenNiYomg[JVidCCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmgdoVt[XSrdnWgeI8h[2:wdILvcEwhUUN3MDC9JFI1NjRizszNMi=> MlW5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NUC0PFkoRjJ2OUWwOFg6RC:jPh?=
DLD1 MknRSpVv[3Srb36gZZN{[Xl? MkjkNVAhfU1? NHPQeY1KdmS3Y4Tpc44hd2ZiQYjpclIhcW5iaIXtZY4hTEyGMTDj[YxteyCxdnXy[ZhxemW|c3nu[{BKX1JvSWOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iV370JJBifGi5YYmgZYN1cX[rdImgZZQhOTBidV2gZpkhW1SIIILldI9zfGW{IHHzd4F6KG2ndHjv[C=> NIXtNXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGyOVE2Pid-MUmxNlUyPTZ:L3G+
HEK293 NFqyRoRHfW6ldHnvckBie3OjeR?= NHH6Z2NFcXOybHHj[Y1mdnRib3[gTXdTNVCEIH\yc40hSXirbkKgbY4hUEWNMkmzJINmdGy|IHL5JHdme3Sncn6gZoxwfCCvZYToc4Q> NYrBSmNHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNlUyPTZpPkG5NVI2OTV4PD;hQi=>
DLD1 NXXTc5JSTnWwY4Tpc44h[XO|YYm= MYrJcohq[mm2aX;uJI9nKEG6aX6yJJBzd3SnaX6g[IVoemGmYYTpc44hcW5iaIXtZY4hTEyGMTDj[YxteyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> M1\RU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK1NVU3Lz5zOUGyOVE2PjxxYU6=
DLD1 M2LUZWZ2dmO2aX;uJIF{e2G7 NWf5PJlwUW6mdXP0bY9vKG:oIFH4bY4zKGGlY4XteYxifGmxbjDpckBpfW2jbjDEUGQyKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIGTodlQyKHCqb4PwbI9zgWyjdHXkJIJmfGFvY3Hz[YlvKGyndnXsd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= M3nsSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK1NVU3Lz5zOUGyOVE2PjxxYU6=
DLD1 NEnSeVNHfW6ldHnvckBie3OjeR?= MkDDTY5lfWO2aX;uJI9nKEG6aX6yJIFk[3WvdXzheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKFOnckO3JJBpd3OyaH;yfYxifGWmIHLleIEu[2G|ZXnuJIxmfmWuczDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? NWHlepZlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNlUyPTZpPkG5NVI2OTV4PD;hQi=>
DLD1 MkfaSpVv[3Srb36gZZN{[Xl? M4rjb2Rm[3KnYYPlJIlvKGKndHGtZ4F{\WmwIHHjZ5VufWyjdHnvckBqdiCqdX3hckBFVERzIHPlcIx{KGW6cILld5NqdmdiaX6gRXBEKG23dHHueC=> NUPGSJo1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNlUyPTZpPkG5NVI2OTV4PD;hQi=>
DLD1 NEj4NHZHfW6ldHnvckBie3OjeR?= NUG2XmZpOiCqcoO= MWXJcoR2[3Srb36gc4YhSXirbkKgd5Ri[mmuaYrheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPl[EB1emGwc3PybZB1cW:wIH;mJGF5cW5{IHHmeIVzKDJiaILzJIJ6KFKWLWDDVkBu\XSqb3S= M{XPblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK1NVU3Lz5zOUGyOVE2PjxxYU6=
DLD1 NXPPemg3TnWwY4Tpc44h[XO|YYm= M3XqWWlv\HWldHnvckBw\iCDeHnuNkBi[2O3bYXsZZRqd25iaX6gbJVu[W5iRFzENUBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCocnXlJIJmfGFvY3Hz[YlvKGyndnXsd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= Mn7QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzMkWxOVYoRjF7MUK1NVU3RC:jPh?=
DLD1 MX3GeY5kfGmxbjDhd5NigQ>? NHji[ndKdmS3Y4Tpc44hd2ZiQYjpclIhe3SjYnnsbZpifGmxbjDpckBpfW2jbjDEUGQyKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gW451N2KndHGtZ4F{\WmwIIDheIh4[Xl? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF{NUG1Okc,OTlzMkWxOVY9N2F-
DLD1 NHLsU5lHfW6ldHnvckBie3OjeR?= MkHCTY5lfWO2aX;uJI9nKEG6aX6yJIFk[3WvdXzheIlwdiCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKFOnckOzJJBpd3OyaH;yfYxifGWmIHLleIEu[2G|ZXnuJIxmfmWuczDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF{NUG1Okc,OTlzMkWxOVY9N2F-
DLD1 NFLaUJRHfW6ldHnvckBie3OjeR?= M2HmZmlv\HWldHnvckBw\iCDeHnuNkBi[2O3bYXsZZRqd25iaX6gbJVu[W5iRFzENUBk\WyuczDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? M{T0d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK1NVU3Lz5zOUGyOVE2PjxxYU6=
DLD1 NW\5RnlZTnWwY4Tpc44h[XO|YYm= NHHsO5QyKHSxIEKwJJVO NH3kbpMzPCCqcoO= MnfFTY5pcWKrdHnvckBw\iC2YX7rfZJie2ViaX6gbJVu[W5iRFzENUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGTDSk1l\XCnbnTlcpQhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIF1KDFidH:gNlAhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 NGn2cpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWyO|c6Oid-MkS1Nlc4QTJ:L3G+
DLD1 MX7DfZRwfG:6aXPpeJkh[XO|YYm= MoflNUB1dyB{MDD1US=> MkXFNVAh\GG7cx?= M{TNTGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRNTDFiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYYSgNUB1dyB{MDD1UUBu\WG|dYLl[EBwdiCmYYmgNVAh[nliY4L5d5RidCC4aX;s[ZQhe3SjaX7pcoc> NIj4NHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWyO|c6Oid-MkS1Nlc4QTJ:L3G+
HT29 MWHGeY5kfGmxbjDhd5NigQ>? NG[zXY8zPSC3TR?= NIjj[lEzPCCqcoO= M2[zNWlvcGmkaYTpc44hd2ZiV370JJNq\26jbHnu[{BqdiCqdX3hckBJXDJ7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[mW2YT3jZZRmdmmwLX3l[IlifGWmIGTj[k9N\WZidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGG2IEK1JJVOKGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9t NWTs[ZZuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OVA1QDlpPkK0PVUxPDh7PD;hQi=>
HT29 M3n5OWZ2dmO2aX;uJIF{e2G7 M3\lfVI2KHWP M3;vTVI1KGi{cx?= MVvJcohq[mm2aX;uJI9nKFewdDDzbYdv[WyrbnegbY4hcHWvYX6gTHQzQSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIJmfGFvY3H0[Y5qdi2vZXTpZZRm\CCWY3[vUIVnKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBifCB{NTD1UUBi\nSncjCyOEBpenNiYomg[JVidCCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmgdoVt[XSrdnWgeI8h[2:wdILvcEBqdiCycnXz[Y5k\SCxZjDHV2suO2KndHGgbY5pcWKrdH;yJGxqS2x? NVjBWmZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OVA1QDlpPkK0PVUxPDh7PD;hQi=>
HT29 NWrsNWRFTnWwY4Tpc44h[XO|YYm= M3jzdVI2KHWP M3Ptd|I1KGi{cx?= NEjtdplKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIFjUNlkh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iYYjpclIhdVKQQTDlfJBz\XO|aX;uJIF1KDJ3IIXNJIFnfGW{IEK0JIhzeyCkeTDxeYFvfGm2YYTpeoUhemWjbD30bY1mKFCFUjDhd5NigQ>? MoHjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NUC0PFkoRjJ2OUWwOFg6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
E-cadherin / N-cadherin / Snail / Vimentin; 

PubMed: 26450645     


Western blot analyses showing protein expression patterns of the EMT markers according to IWR-1 dose and exposure time. β-Actin was used as a loading control.

p-Akt / Akt; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the phosphorylation of Akt in a concentration- and time-dependent manner, respectively.

Survivin; 

PubMed: 26450645     


Western blot analysis showing that IWR-1 decreased the survivin expression in a concentration- and time-dependent manner, respectively.

26450645

Protocol

Solubility (25°C)

In vitro DMSO 21 mg/mL (51.28 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 409.44
Formula

C25H19N3O3

CAS No. 1127442-82-3
Storage powder
in solvent
Synonyms endo-IWR 1
Smiles C1C2C=CC1C3C2C(=O)N(C3=O)C4=CC=C(C=C4)C(=O)NC5=CC=CC6=C5N=CC=C6

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID