Home Stem Cells & Wnt Wnt/beta-catenin inhibitor Adavivint (SM04690) For research use only.

Adavivint (SM04690)

Synonyms: Lorecivivint

Adavivint (SM04690,Lorecivivint) is a potent and specific inhibitor of canonical Wnt signaling with an EC50 of 19.5 nM for inhibiting the TCF/LEF reporter. It is ∼150- to 500-fold more potent than the other known Wnt inhibitors across multiple cellular assays.

Adavivint (SM04690) Chemical Structure

Adavivint (SM04690) Chemical Structure

CAS: 1467093-03-3

Selleck's Adavivint (SM04690) has been cited by 6 publications

Purity & Quality Control

Batch: S876101 DMSO] 30 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 98.13%
98.13

Adavivint (SM04690) Related Products

Signaling Pathway

Wnt/beta-catenin Signaling Pathways

Wnt/beta-catenin Signaling Pathway

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Biological Activity

Description Adavivint (SM04690,Lorecivivint) is a potent and specific inhibitor of canonical Wnt signaling with an EC50 of 19.5 nM for inhibiting the TCF/LEF reporter. It is ∼150- to 500-fold more potent than the other known Wnt inhibitors across multiple cellular assays.
Targets
Wnt [1]
In vitro
In vitro

Bone-marrow-derived hMSCs (CD29+, CD44+ CD166+ CD105+ CD45-), treated with SM04690, show a dose-dependent decrease in the expression of Wnt pathway genes (ASCL1, LEF1, TCF7L2, TCF7, C-262 MYC and AXIN2). M04690 also inhibits the expression of AXIN2, TCF7 and LEF1 in hMSCs and AXIN2 and LGR5 in IEC6 (intestinal stem cells sensitive to Wnt activation) when the Wnt pathway is selectively activated using either Wnt3a or a GSK3β inhibitor, CHIR-99021. SM04690 has minimal effects on the non-canonical Wnt pathway and the BMP pathway. SM04690 protects chondrocytes from catabolic breakdown in vitro[1].
Cell Research Cell lines SW480 cells and hMSCs
Concentrations 10, 30 and 100 nM
Incubation Time 48 h
Method

--
In Vivo
In vivo

Intra-articular injection of SM04690 promotes cartilage growth and improves joint health in a rat model of knee osteoarthritis. It decreases cartilage breakdown in vivo[1].
Animal Research Animal Models Sprague-Dawley (SD) rats (12 weeks old, male)
Dosages 0.3μg in 50μl
Administration intra-articular injection
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03727022 Completed
Knee Osteoarthritis
Biosplice Therapeutics Inc.
 November 28 2018 Phase 2
NCT03246399 Terminated
Degenerative Disc Disease
Biosplice Therapeutics Inc.
 July 26 2017 Phase 1

Chemical Information & Solubility

Molecular Weight 505.55 Formula

C29H24FN7O
CAS No. 1467093-03-3 SDF --
Smiles CC(C)CC(=O)NC1=CN=CC(=C1)C2=CC3=C(C=C2)NN=C3C4=NC5=C(N4)C=NC=C5C6=CC(=CC=C6)F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 30 mg/mL ( (59.34 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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