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Isoquercitrin Wnt/beta-catenin inhibitor

Name: Isoquercitrin
Brand: Selleck Chemicals
SKU: S3842
Availability: InStock
Rating: 5 (6 reviews)

Cat.No.S3842

Isoquercitrin (Hirsutrin, 3-Glucosylquercetin, Quercetin 3-o-glucopyranoside), a flavonoid compound with anticancer activity isolated from Bidens bipinnata L, is an inhibitor of Wnt/β-catenin that acts downstream of the β-catenin nuclear translocation.

Chemical Structure

Molecular Weight: 464.38

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.71%

99.71

Related Targets	JAK STAT TGF-beta/Smad ERK GSK-3 Casein Kinase Notch ROCK Hedgehog/Smoothened PKA Secretase PORCN OCT KLF Stemness kinase SFRP Fascin
Related Products	ICG-001 IWR-1-endo IWP-2 SKL2001 BML-284 (Wnt agonist 1) Foscenvivint (PRI-724) Salinomycin (from Streptomyces albus) FH535 PNU-74654 LF3 KYA1797K KY02111 iCRT3 Adavivint (SM04690) MSAB IQ-1 iCRT14 CCT251545 JW55 Methyl Vanillate M435-1279 WAY-262611 JW74 Non-phospho β-Catenin (Ser45) Antibody [K20N13] Zamaporvint (RXC004) DKK1 Antibody [K16J6] Tegatrabetan (BC-2059) TCF4/TCF7L2 Antibody [P1D24] TCF3/TCF7L1 Antibody [G18A14] Phospho-β-Catenin (Ser552) Antibody [K9A16]

Chemical Information, Storage & Stability

Molecular Weight	464.38	Formula	C₂₁H₂₀O₁₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	482-35-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Hirsutrin, 3-Glucosylquercetin, Quercetin 3-o-glucopyranoside			Smiles	C1=CC(=C(C=C1C2=C(C(=O)C3=C(C=C(C=C3O2)O)O)OC4C(C(C(C(O4)CO)O)O)O)O)O

Solubility

In vitro
Batch:

DMSO : 92 mg/mL (198.11 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.650mg/ml (10.01mM)	Taking the 1 mL working solution as an example, add 50 μL of 93 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.450mg/ml (0.97mM)	Taking the 1 mL working solution as an example, add 50 μL of 9 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	Wnt/β-catenin ^[1]
In vitro	Isoquercitrin exerts inhibitory effect on Wnt/β-catenin signaling and no toxic effects on colon cancer cell lines. It supresses the proliferation of colon cancer cells but does not affect non-tumoral colon cells. This compound inhibits cell migration in colon cancer cells, but has also no apparent effect on the non-tumor colon cells. It inhibits the signaling downstream of β-catenin nuclear translocation as this chemical reverses the effect of β-catenin S33A, which cannot be phosphorylated and avoids degradation^[1].
In vivo	Isoquercitrin treatment significantly affects Xenopus embryo development, most likely by disturbing axial patterning. This compound affects canonical Wnt signaling downstream of β-Catenin nucleus translocation^[1]. In animals receiving this chemical, eosinophil counts are lower in the bronchoalveolar lavage fluid (BALF), blood and lung pa-renchyma. Neutrophil counts in blood and IL-5 levels in lung homogenate are lower in treated mice. No alterations in mononuclear cell numbers are observed. It also demonstrates anti-asthmatic activity by inhibiting of carbachol- and leukotriene D4-induced contraction in guinea pig airways^[2].
References	[1] https://pubmed.ncbi.nlm.nih.gov/25359775/ [2] https://pubmed.ncbi.nlm.nih.gov/18026696/

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