JW55

For research use only.

Catalog No.S6745

2 publications

JW55 Chemical Structure

CAS No. 664993-53-7

JW55 is a potent and selective inhibitor of the canonical Wnt pathway that functions via inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).

Selleck's JW55 has been cited by 2 publications

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Biological Activity

Description JW55 is a potent and selective inhibitor of the canonical Wnt pathway that functions via inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).
Targets
Wnt [1]
()
In vitro

JW55 inhibits the PARP domain of TNKS1/2, leading to the stabilization of AXIN2 followed by increased degradation of β-catenin. Wnt3a-induced HEK293 cells containing a transiently transfected ST-Luc (SuperTop-luciferase) reporter show inhibition by JW55 with an IC50 value of 470 nmol/L. JW55 decreases auto-PARsylation of TNKS1/2 in vitro with IC50 values of 1.9 μmol/L and 830 nmol/L, respectively. JW55-mediated inhibition of canonical Wnt signaling results in reduced cell-cycle progression, proliferation, and colony formation in the CRC cell line SW480 in vitro[1].

In vivo

JW55 reduces XWnt8-induced axis duplication in Xenopus embryos and tamoxifen-induced polyposis formation in conditional APC mutant mice[1].

Protocol

Cell Research:

[1]

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  • Cell lines: SW480 cells
  • Concentrations: 10, 5, or 1 μmol/L
  • Incubation Time: 9 days
  • Method:

    --


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Apc(CKO/CKO)Lgr5-CreERT2+ mice
  • Dosages: 100 mg/kg
  • Administration: oral
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 87 mg/mL (200.23 mM)
Water Insoluble
Ethanol '4 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 434.49
Formula

C25H26N2O5

CAS No. 664993-53-7
Storage powder
in solvent
Synonyms N/A
Smiles COC1=CC=C(C=C1)C2(CCOCC2)CNC(=O)C3=CC=C(C=C3)NC(=O)C4=CC=CO4

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID