JW55

Catalog No.S6745

For research use only.

JW55 is a potent and selective inhibitor of the canonical Wnt pathway that functions via inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).

JW55 Chemical Structure

CAS No. 664993-53-7

Selleck's JW55 has been cited by 2 Publications

Purity & Quality Control

Choose Selective Wnt/beta-catenin Inhibitors

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Biological Activity

Description JW55 is a potent and selective inhibitor of the canonical Wnt pathway that functions via inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).
Targets
Wnt [1]
()
In vitro

JW55 inhibits the PARP domain of TNKS1/2, leading to the stabilization of AXIN2 followed by increased degradation of β-catenin. Wnt3a-induced HEK293 cells containing a transiently transfected ST-Luc (SuperTop-luciferase) reporter show inhibition by JW55 with an IC50 value of 470 nmol/L. JW55 decreases auto-PARsylation of TNKS1/2 in vitro with IC50 values of 1.9 μmol/L and 830 nmol/L, respectively. JW55-mediated inhibition of canonical Wnt signaling results in reduced cell-cycle progression, proliferation, and colony formation in the CRC cell line SW480 in vitro[1].

In vivo

JW55 reduces XWnt8-induced axis duplication in Xenopus embryos and tamoxifen-induced polyposis formation in conditional APC mutant mice[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: SW480 cells
  • Concentrations: 10, 5, or 1 μmol/L
  • Incubation Time: 9 days
  • Method:

    --

Animal Research:

[1]

  • Animal Models: Apc(CKO/CKO)Lgr5-CreERT2+ mice
  • Dosages: 100 mg/kg
  • Administration: oral

Solubility (25°C)

In vitro

DMSO 87 mg/mL
(200.23 mM)
Water Insoluble
Ethanol '4 mg/mL

Chemical Information

Molecular Weight 434.49
Formula

C25H26N2O5

CAS No. 664993-53-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC=C(C=C1)C2(CCOCC2)CNC(=O)C3=CC=C(C=C3)NC(=O)C4=CC=CO4

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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