IWP-2

For research use only. Not for use in humans.

Catalog No.S7085

22 publications

IWP-2 Chemical Structure

Molecular Weight(MW): 466.6

IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses.

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Selleck's IWP-2 has been cited by 22 publications

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Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses.
Features Selective inhibitor of Porcn-mediated Wnt secretion.
Targets
Wnt [1]
(Cell-free assay)
27 nM
In vitro

IWP-2 is useful in both regenerative medicine and anticancer efforts. IWP-2 inactivates Porcn, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. IWP-2 blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. [1]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse L cells NGjMcJBHfW6ldHnvckBie3OjeR?= M4\HXlI1KGh? NFTkWm5KdmirYnn0bY9vKG:oIIDvdoN2eGmwZT3t[YRq[XSnZDDXcpQhe2mpbnHscIlv\yCrbjDtc5V{\SCOIHPlcIx{KGGodHXyJFI1KGi{czDifUBUeHKrbnfldmlu[Wencz3Uc5BndGG|aDDy[ZBwenSncjDhd5NigSxiRVO1NF0{OCCwTR?= MoLmNlM1Pzd|NkW=

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
Wnt5-a/b / β-catenin / Axin2; 

PubMed: 27119499     


A549 cells were harvested, and Western blot analysis was conducted for β-catenin, Wnt5-a/b, NKD2, and Axin2 with or without IWP-2

c-myc / Cyclin D1 / Survivin; 

PubMed: 23761839     


Western blot for key regulators of the Wnt/β-catenin signaling pathway. The cells were treated with IWP-2 for 3 days. *P<0.05, **P<0.01 and ***P<0.001.

MMP7; 

PubMed: 24325363     


β-catenin inhibitor inhibited the expression of MMP7 in Bel7402 cells. Bel7402 cells were treated without or with various concentration of β-catenin inhibitor (IWP-2) for 24 h, and then the whole cell lysates were analyzed by western blotting for MMP7 exp䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖

27119499 23761839 24325363

Protocol

Cell Research:

[1]
- Collapse
  • Cell lines: L-Wnt-STF cells
  • Concentrations: 5 μM
  • Incubation Time: 24 h
  • Method:

    L-Wnt-STF cells that exhibit constitutive Wnt pathway activation were incubated with IWR (10μM) and IWP (5μM) compounds for 24 hrs prior to lysis. Cellular lysates were subjected to Western blot analysis.


    (Only for Reference)
Animal Research:

[2]
- Collapse
  • Animal Models: Crl:CD1 (ICR) and FVB/N female mice
  • Formulation: encapsulated in liposomes
  • Dosages: 50 µg
  • Administration: s.c.
    (Only for Reference)

Solubility (25°C)

In vitro DMF 22 mg/mL warmed (47.14 mM)
DMSO 4 mg/mL warmed (8.57 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 466.6
Formula

C22H18N4O2S3
CAS No. 686770-61-6
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CC2=C(C=C1)N=C(NC(=O)CSC3=NC4=C(SCC4)C(=O)N3C5=CC=CC=C5)S2

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID