For research use only.

Catalog No.S7484

14 publications

FH535 Chemical Structure

CAS No. 108409-83-2

FH535 is a Wnt/β-catenin signaling inhibitor and also a dual PPARγ and PPARδ antagonist.

Selleck's FH535 has been cited by 14 publications

4 Customer Reviews

  • Ki-67 staining in xenograft tissues from FH535-treated and control group. Magnifications: ×200 in left column, ×400 in right column.

    J Cancer, 2017, 8(16):3142-3153. FH535 purchased from Selleck.

  • (C and D) Western bolt analysis of proteins in CSN6 knockdown, DMSO and FH535 treated cells.

    Cancer Med, 2018, 7(2):285-296. FH535 purchased from Selleck.

  • H. HCCLM3 si-THOR or SMMC7721si-THOR and their control cells were treated with FH535 (40 nM) or not and CD133+ or EpCAM+ hepatoma cells was checked by flow-cytometric assay. I. HCCLM3 si-THOR or SMMC7721si-THOR and their control cells were treated with FH535 (40 nM) or not and subjected to spheroid formation.

    Gene, 2019, 684:95-103. FH535 purchased from Selleck.

  • BrdU assay was used to detect the proliferation level of cells in four groups: the control group, FH535 (15 μM) group, NAM (50 mM) + FH535 (15 μM) group, REV (100 μM) + FH535 (15 μM) group. A. At the next day, the cells were fixed and immunostained for BrdU (red) and with DAPI (blue) then merged the two pictures of the same field. The scale bar is 100 μm. B. The total numbers of cells (DAPI-staining cells) per field in the four groups. C. BrdU-positive rate of C2C12 cells was analyzed. Each value represents the mean ± standard error of the mean of triplicate determinations from three independent cell preparations. *P<0.05, **P<0.01, ***P<0.001 vs the value of the control; n=3; error bars ± standard error of means. Statistical analysis was conducted using one-way ANOVA. Original magnification is ×200.

    Int J Clin Exp Pathol, 2016, 9(3):2857-2868.. FH535 purchased from Selleck.

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Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description FH535 is a Wnt/β-catenin signaling inhibitor and also a dual PPARγ and PPARδ antagonist.
Wnt/β-catenin [1] PPARγ [1] PPARδ [1]
In vitro

FH535 antagonizes β-Catenin/Tcf–mediated transcription, and inhibits recruitment of the coactivators GRIP1 and β-catenin to PPARδ and PPARγ. FH535 shows selective anti-proliferation effect on some cancer cells expressing high or active Wnt/β-catenin pathway. [1] FH535 increases cigarette smoke condensate cytotoxicity, and causes changes in β-catenin and EGR-1 signaling. [2] FH535 has potential therapeutic value in treatment of liver cancer by targeting liver cancer stem cells and hepatocellular carcinoma cell lines. [3]


Kinase Assay:[1]
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High-throughput Library Screen:

Three copies of the optimized or mutated Tcf-binding element from TOPFLASH or FOPFLASH driving a secreted alkaline phosphatase reporter gene are cloned into pCEP4 plasmid, replacing the cytomegalovirus promoter. The plasmids are transfected into HepG2 cells, and hygromycin-resistant clones are pooled. Library screening is done at 20 μmol/L concentration in HepG2 serum-free media. Hits are tested in the HCT116 cell line for inhibition of TOPFLASH luciferase activity but not for inhibition of a reporter activity controlled from β-actin promoter.
Cell Research:[1]
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  • Cell lines: HCT116, SW48, RKO, LoVo, COLO205, IEC6, A427, HCC15, NCI-H1703, A549, HepG2, Hep3b, Huh7, Fibroblasts
  • Concentrations: 30 μM
  • Incubation Time: 48 hours
  • Method: Cell viability is determined by the modified 3H-thymidine incorporation assay. Briefly, cells are plated in 96-well microplates for 24 h and treated in triplicate with various concentrations of the test compound. After 48 h of compound exposure, the cells are incubated for an additional 48 h in compound-free medium. The cells are then incubated in medium containing 3H-thymidine for 24 h, washed and mixed with the scintillant in the 96-well plate. Individual wells are counted with a 96-well scintillation counter and the LC50 is calculated.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 72 mg/mL warmed (199.33 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 361.20


CAS No. 108409-83-2
Storage powder
in solvent
Synonyms N/A
Smiles CC1=C(C=CC(=C1)[N+](=O)[O-])NS(=O)(=O)C2=C(C=CC(=C2)Cl)Cl

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID