Methyl Vanillate

Catalog No.S3630

For research use only.

Methyl Vanillate, one of the ingredients in Hovenia dulcis Thunb, activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro.

Methyl Vanillate Chemical Structure

CAS No. 3943-74-6

Selleck's Methyl Vanillate has been cited by 1 Publication

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Biological Activity

Description Methyl Vanillate, one of the ingredients in Hovenia dulcis Thunb, activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro.
Targets
Wnt/β-catenin [1]
()
In vitro

Methyl vanillate (MV) activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro. It increases the expression of differentiation markers RUNX2, BMP2, ALP, and OCN in a dose-dependent manner. MV decreases and increases the expression of RANKL and OPG, respectively. Methyl vanillate dose-dependently elevates ALP activity in calvarial osteoblasts[1].

In vivo Methyl vanillate rescues trabecular or cortical femoral bone loss in the ovariectomized mice without inducing any significant weight changes or abnormality in liver tissue when administrated orally[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Calvarial osteoblasts
  • Concentrations: 10 and 20 μM
  • Incubation Time: 24 h
  • Method: Calvarial osteoblasts are seeded onto a coverslip in 12-well plates at a density of 3×104 cells/well. After 24 h, HDT extract or MV is added for another 24 h and the cells are subsequently fixed with 4% PFA for 10 min. Cells are permeabilized with 0.2% Triton X-100 for 15 min and incubated for 30 min with 5% bovine serum albumin (BSA) blocking solution. The cells are incubated with the primary antibody (β-catenin) overnight at 4°C and then washed with PBS three times. Cells are then incubated with Alexa Fluor 502-conjugated IgG secondary antibody is incubated for 1 h, followed by incubation with DAPI for 5 min. The cells are mounted in Gel/Mount media. The fluorescence signal is captured using confocal microscopy.
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: ICR mice
  • Dosages: 25 and 100 mg/kg
  • Administration: oral
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 36 mg/mL
(197.61 mM)
Ethanol 36 mg/mL
(197.61 mM)
Water 12 mg/mL
(65.87 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 182.17
Formula

C9H10O4

CAS No. 3943-74-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=C(C=CC(=C1)C(=O)OC)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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