Salinomycin (from Streptomyces albus)

Catalog No.S8129 Synonyms: Coxistac, Bio-cox

Salinomycin (from Streptomyces albus) Chemical Structure

Molecular Weight(MW): 751.00

Salinomycin, traditionally used as an anti-coccidial drug, has recently been shown to possess anti-cancer and anti-cancer stem cell (CSC) effects.

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2 Customer Reviews

  • The salinomycin inhibited the growth of CNE-2 in time- and dose-dependent manner. SAL salinomycin

    Tumour Biol, 2016, 305-11. Salinomycin (from Streptomyces albus) purchased from Selleck.

    Drug sensitivity of KPC tumor cell subpopulations. CD133, Aldefluor, and Sca-1-positive or -negative cells or unsorted KPC cells were cultured in the presence of increasing concentrations of salinomycin. After 72 hours, cell viability was assessed using CellTiter Glo reagent, and percent viability was normalized to vehicle-treated controls. Pair-wise multiple comparisons were performed with one-way ANOVA with Bonferroni adjustment. P values < 0.05 were considered statistically significant.

    Tumor and Stem Cell Biology, 2015, 75(21):4582-4592.. Salinomycin (from Streptomyces albus) purchased from Selleck.

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Biological Activity

Description Salinomycin, traditionally used as an anti-coccidial drug, has recently been shown to possess anti-cancer and anti-cancer stem cell (CSC) effects.
Targets
Wnt/β-catenin [1]
()
In vitro

Salinomycin, a polyether ionophore antibiotic isolated from Streptomyces albus, has been shown to kill CSCs in different types of human cancers, most likely by interfering with ABC(ATP-binding cassette) drug transporters, the Wnt/β-catenin signaling pathway, and other CSC pathways. Salinomycin exhibits antimicrobial activity against Gram-positive bacteria including Bacillus subtilis, Staphylococcus aureus, Micrococcus flavus, Sarcina lutea, Mycobacterium spp., some filamentous fungi, Plasmodium falciparum, and Eimeria spp., protozoan parasites responsible for the poultry disease coccidiosis. In addition, salinomycin has early been shown to act in different biological membranes, including cytoplasmic and mitochondrial membranes, as a monovalent cation ionophore with strict selectivity for alkali ions and a strong preference for K+, thereby promoting mitochondrial and cytoplasmic K+ efflux and inhibiting oxidative phosphorylation. Salinomycin can induce massive apoptosis in human cancer cells of different origin that display multiple mechanisms of drug and apoptosis resistance[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HL60 cells NVjNcIhOWHKxbHnm[ZJifGmxbjDhd5NigQ>? Mn[2O|IhcA>? MoTnRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGy|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6yPUDPxE1? NV;zT2d{OjV4NES2O|Q>
MV4-11 cells MWjQdo9tcW[ncnH0bY9vKGG|c3H5 MoHFO|IhcA>? NGHO[W5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3WOE0yOSClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:OC5|MzFOwG0> M1jpUFI3OTZ|MUm3
LoVo cells MlrDVJJwdGmoZYLheIlwdiCjc4PhfS=> MXi3NkBp MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyxVn:gZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IGPSRkBu\XSqb3SsJGlEPTB;MD6zOkDPxE1? M4Dyd|I2PjR2Nke0
VCaP cells M1L1T3Bzd2yrZnXyZZRqd25iYYPzZZk> NGrjTFlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIG\DZXAh[2WubIOsJGVEPTB;MD6zPEDPxE1? MknHNlMxPjN2MEC=
LS180 cells NWT5OItGWHKxbHnm[ZJifGmxbjDhd5NigQ>? Ml\tO|IhcA>? MYjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyVMUiwJINmdGy|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDTVmIhdWW2aH;kMEBKSzVyPUCuOlEh|ryP NHjYNXIzPTZ2NE[3OC=>
HT-29 cells NIrpO2dRem:uaX\ldoF1cW:wIHHzd4F6 MX23NkBp NFvzT5hCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBUWkJibXX0bI9lNCCLQ{WwQVEvODNizszN NEntWFkzPTZ2NE[3OC=>
SW707 cells NWfoRVdEWHKxbHnm[ZJifGmxbjDhd5NigQ>? MkDVO|IhcA>? MlLIRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{ewO{Bk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjC3NkBpenNiYomgV3JDKG2ndHjv[EwhUUN3ME2xMlc{KM7:TR?= MlnFNlU3PDR4N{S=
HL60/Vinc cells NVTpfYNMS3m2b4TvfIlkcXS7IHHzd4F6 NX\YeFFrPzJiaB?= NYDHbGNtS3m2b4TvfIlkcXS7IHHnZYlve3RidnnuZ5Jqe3SrbnWtdoV{cXO2YX70JIh2dWGwIFjMOlAwXmmwYzDj[YxteyCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9N{41PCEQvF2= NGDZV24zOzB5OUWyNy=>
BALB/3T3 cells NYfGcHA2S3m2b4TvfIlkcXS7IHHzd4F6 MUm3NkBp MYjDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDCRWxDNzOWMzDj[YxteyCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlgvODhizszN M4G0eVI{ODd7NUKz

... Click to View More Cell Line Experimental Data

In vivo Salinomycin is able to effectively eliminate CSCs and to induce partial clinical regression of heavily pretreated and therapy-resistant cancers. It has also been demonstrated as a positive ionotropic and chronotropic agent that increased cardiac output, left ventricular systolic pressure, heart rate, mean arterial pressure, coronary artery vasodilatation and blood flow, and plasma catecholamine concentration. These results have been obtained in experiments with mongrel dogs that has received a single intravenous injection of 150 μg/kg salinomycin. However, It has been reported with a considerable toxicity of salinomycin in mammals, such as horses, pigs, cats, and alpacas after accidental oral or inhalative intake. Risk assessment data recently published by the European Food Safety Authority declare an acceptable daily intake (ADI) of 5 μg/kg salinomycin for humans, because daily intake of more than 500 μg/kg salinomycin by dogs leads to neurotoxic effects, such as myelin loss and axonal degeneration. Intravenous administration of 200-250 μg/kg salinomycin every second day for three weeks results in partial regression of tumor metastasis and shows only minor acute and long-term side effects, but no severe acute and long-term side effects observed with conventional chemotherapeutic drugs[1].

Protocol

Cell Research:

[2]

+ Expand
  • Cell lines: colon cancer (SW480, SW620, RKO) and breast cancer cell lines (MCF-7, T47D, MDA-MB-453).
  • Concentrations: 1, 2.5, 5, 10 μM
  • Incubation Time: 26 h
  • Method:

    Cells are plated at 1500 (SW480 or SW620) or 4000 (all other cell lines) per well in flat-bottom 96-well plates. They are treated with salinomycin at the indicated concentration 16 hours later. Seventy-two hours after this, 5 mg/ml MTT in PBS is added per well; cells are lysed after 4 hours by addition of 50 µl triplex solution (10% SDS; 5% isobutanol, 0.012 M HCl). Absorbance is measured at 562 nm. Alternatively, viability is determined with the ViaCount reagent on a Guava easyCyte8HT flow cytometer, following the instructions of the manufacturer.


    (Only for Reference)
Animal Research:

[3]

+ Expand
  • Animal Models: NOD/SCID mice
  • Formulation: 5% ethanol
  • Dosages: 5 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (133.15 mM)
Ethanol 79 mg/mL (105.19 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 751.00
Formula

C42H70O11

CAS No. 53003-10-4
Storage powder
in solvent
Synonyms Coxistac, Bio-cox

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID