Isoxazole 9 (ISX-9)

For research use only.

Catalog No.S7914

Isoxazole 9 (ISX-9) Chemical Structure

CAS No. 832115-62-5

Isoxazole 9 (Isx-9) is a synthetic promotor of adult neurogenesis by triggering neuronal differentiation of adult neural stem/precursor cells (NSPCs). Isoxazole 9 (Isx-9) activates multiple pathways including TGF-β induced epithelial–mesenchymal transition (EMT) signaling, canonical and non-canonical Wnt signaling at different stages of cardiac differentiation.

Purity & Quality Control

Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description Isoxazole 9 (Isx-9) is a synthetic promotor of adult neurogenesis by triggering neuronal differentiation of adult neural stem/precursor cells (NSPCs). Isoxazole 9 (Isx-9) activates multiple pathways including TGF-β induced epithelial–mesenchymal transition (EMT) signaling, canonical and non-canonical Wnt signaling at different stages of cardiac differentiation.
In vitro

In NSPCs, isoxazole 9 increases cell number, and promotes cell differentiation. In OPCs, isoxazole 9 induces cell damage. In outgrowth EPCs, isoxazole 9 decreases tube formation without effect on early EPCs. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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HCN MofMSpVv[3Srb36gZZN{[Xl? MkLsOUB2VQ>? NXy1NFA5UW6mdXP0bY9vKG:oIF7NSGEhemWlZYD0c5IudWWmaXH0[YQhS2F{KzDpcoZtfXhiaX6gdoF1KEiFTjDj[YxteyCjdDC1JJVOKGK7IF\1doEuOiCrbXHnbY5oKGGwYXz5d4l{KGmwIIDy[ZNmdmOnIH;mJG5OTEFicnXj[ZB1d3JiYX70ZYdwdmm|dDDNT|gxOQ>? NEm1O4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW1Nlg{Oid-MUi1OVI5OzJ:L3G+
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HCN M4PUNGZ2dmO2aX;uJIF{e2G7 M3S5OFUxKHWP Mlm5NlQhcHK| NXjJO3RNUW6mdXP0bY9vKG:oIF7NSGEhemWlZYD0c5IudWWmaXH0[YQhdmW3cn;EJIdmdmViZYjwdoV{e2mxbjDpckBz[XRiSFPOJINmdGy|IHH0JFUxKHWPIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMhcW5icILld4Vv[2Vib3[gUm1FSSC{ZXPldJRweiCjboTh[49vcXO2IH7p[oVlcXCrbnW= M1yx[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUWyPFMzLz5zOEW1Nlg{OjxxYU6=
HCN NHz3eXhHfW6ldHnvckBie3OjeR?= MkX0OVAhfU1? MYmyOEBpenN? M3jEXGlv\HWldHnvckBw\iCOLYT5dIUh[2GuY3n1cUBkcGGwbnXsM25OTEFicnXj[ZB1d3JvbXXkbYF1\WRibnX1do9FKGenbnWg[ZhxemW|c3nvckBqdiC{YYSgTGNPKGOnbHzzJIF1KDVyIIXNJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXNiaX6gdJJme2WwY3Wgc4YhdXWudHnwcIUhcW6qaXLpeI9zew>? NV22XXJlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1OVI5OzJpPkG4OVUzQDN{PD;hQi=>
HCN MXjGeY5kfGmxbjDhd5NigQ>? MVK4JJRwKDZ2IIXN NX7lWlVwQSCqcoO= MWnJcoR2[3Srb36gc4YhdmW3cn;n[Y5me2m|IHnuJJVv\GmoZnXy[Y51cWG2ZXSgdoF1KEiFTjDj[YxteyCjc4Pld5Nm\CCjczDNRXAzSUJicILveIVqdiCuZY\lcEBifCB6IITvJFY1KHWPIHHmeIVzKDliaILzJIJ6KHC{b4TlbY4h[myxdITpcoch[W6jbInzbZM> NGPpdIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW1Nlg{Oid-MUi1OVI5OzJ:L3G+
HCN M2S4b2Z2dmO2aX;uJIF{e2G7 NEPYUFNKdmS3Y4Tpc44hd2ZiSFTBR|UhfHKjboPsc4NifGmxbjDpckBkgXSxcHzhd40hd2ZicnH0JGhEViClZXzsd{Bie3Onc4Pl[EBieyCyaH;zdIhwenmuYYTl[EBJTEGFNTDhZ4N2dXWuYYTpc44> M2rVU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUWyPFMzLz5zOEW1Nlg{OjxxYU6=
HCN MkHWSpVv[3Srb36gZZN{[Xl? M2fu[2lv\HWldHnvckBw\iCFQV3LMY1m\GmjdHXkJG1TTXh|IHHjeIl3cXS7IHnuJJJifCCKQ16gZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFIvPSC3TTDwc5Rie3OrdX2gZ4hidm6nbDDpcohq[mm2b4KgT246Og>? NH;mNJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW1Nlg{Oid-MUi1OVI5OzJ:L3G+
HCN NFTic4NHfW6ldHnvckBie3OjeR?= MUS4JJRwKDZ2IIXN MXW5JIhzew>? M1KzdWlv\HWldHnvckBw\iCwZYXyc4dmdmW|aYOgbY4hfW6maX\m[ZJmdnSrYYTl[EBz[XRiSFPOJINmdGy|IHHzd4V{e2WmIHHzJGdtWjJxMzDwdo91\WmwIHzleoVtKGG2IEigeI8hPjRidV2gZYZ1\XJiOTDodpMh[nlicILveIVqdiCkbH;0eIlv\yCjbnHsfZNqew>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV3MkizNkc,OTh3NUK4N|I9N2F-
HCN M2jqdmZ2dmO2aX;uJIF{e2G7 M4T4blYhcHK| NF;oSmNKdmS3Y4Tpc44hd2ZiSFTBR|UheGixc4Doc5J6dGG2aX;uJIlvKHKjdDDIR24h[2WubIOgZYZ1\XJiNjDodpM> MkO2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUK4N|IoRjF6NUWyPFMzRC:jPh?=
HCN M3ruTWZ2dmO2aX;uJIF{e2G7 MUeyOEBpenN? M2T1dGlv\HWldHnvckBw\iCKRFHDOUBxcG:|cHjvdplt[XSrb36gbY4hemG2IFjDUkBk\WyuczDh[pRmeiB{NDDodpM> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV3MkizNkc,OTh3NUK4N|I9N2F-
HCN M2PNNGZ2dmO2aX;uJIF{e2G7 M3zOeWlv\HWldHnvckBw\iCKRFHDOUB1emGwc3zvZ4F1cW:wIHnuJI52[2yndYOgc4YhemG2IFjDUkBk\WyuczDhd5Nme3OnZDDhd{BxcG:|cHjvdplt[XSnZDDISGFEPSCjY3P1cZVt[XSrb36= MlPCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUK4N|IoRjF6NUWyPFMzRC:jPh?=
HCN NH3iTnlHfW6ldHnvckBie3OjeR?= M{LmZ|UhfU1? Mk\mTY5lfWO2aX;uJI9nKEiGQVO1JJRz[W6|bH;jZZRqd25iaX6gcpVkdGW3czDv[kBz[XRiSFPOJINmdGy|IHHzd4V{e2WmIHHzJGdHWC2KRFHDOUBUOjV6QTDTOFk5SSCvdYThcpQh\nW|aX;uJJBzd3SnaX6gZYNkfW23bHH0bY9vKGG2IEWgeW0h[nliZnz1c5Jme2OnbnPlJIF{e2G7 MoC3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUK4N|IoRjF6NUWyPFMzRC:jPh?=
HCN MkHRSpVv[3Srb36gZZN{[Xl? MYm1NEB2VQ>? MkLNN{BpenN? NVfWR5VmUW6mdXP0bY9vKG:oIF7NSGEhemWlZYD0c5IudWWmaXH0[YQhdmW3cn;EJIdmdmViZYjwdoV{e2mxbjDpckBz[XRiSFPOJINmdGy|IHH0JFUxKHWPIHHmeIVzKDNiaILzJIJ6KFKWLWDDVkBidmGueYPpd{BqdiCycnXz[Y5k\SCxZjDOUWRCKHKnY3XweI9zKGGwdHHnc45qe3Ribnnm[YRqeGmwZR?= MkDmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUK4N|IoRjF6NUWyPFMzRC:jPh?=
HCN MUfGeY5kfGmxbjDhd5NigQ>? MnjtNlAhfU1? MlPjTY5lfWO2aX;uJI9nKEiGQVO1JJRz[W6|bH;jZZRqd25iaX6gcpVkdGW3czDv[kBz[XRiSFPOJINmdGy|IHHzd4V{e2WmIHHzJGdHWC2KRFHDOUBnfXOrb36gdJJwfGWrbjDhZ4N2dXWuYYTpc44h[XRiMkCgeW0h[nliZnz1c5Jme2OnbnPlJIF{e2G7 MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV3MkizNkc,OTh3NUK4N|I9N2F-
HCN M4iwcWZ2dmO2aX;uJIF{e2G7 M4G0Xmlv\HWldHnvckBw\iCFQV3LMY1m\GmjdHXkJG1TTXh|IHHjeIl3cXS7IHnuJJJifCCKQ16gZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFIxOCCwTTDQT2MhcW6qaXLpeI9zKEexNkm3Oi=> NHHqWpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW1Nlg{Oid-MUi1OVI5OzJ:L3G+
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... Click to View More Cell Line Experimental Data

In vivo In mice, isoxazole 9 (20 mg/kg, i.p.) crosses the BBB and increases proliferation of neuroblasts and neurogenesis via Mef2-specific mechanisms in the hippocampal SGZ. Isoxazole 9 (20 mg/kg, i.p.) also increases differentiation and dendritic complexity of immature neurons and improves memory. In MWM, isoxazole 9 improves spatial memory. [2]

Protocol

Cell Research:[1]
- Collapse
  • Cell lines: NSPCs, OPCs, early EPCs, and outgrowth EPCs
  • Concentrations: ~50 μM
  • Incubation Time: 48 h
  • Method: Cells are treated with different concentrations of Isx-9 (0, 6.25, 12.5, 25, or 50 μM), and 2 days later, cell numbers are counted in a blinded manner to evaluate the effects of Isx-9 on the proliferation/survival of four types of progenitor/precursor cells.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: Transgenic Nkx2.5-luciferase reporter mice
  • Dosages: 20 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 46 mg/mL (196.35 mM)
Ethanol 13 mg/mL warmed (55.49 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+55% PEG 300+ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 234.27
Formula

C11H10N2O2S

CAS No. 832115-62-5
Storage powder
in solvent
Synonyms N/A
Smiles C1CC1NC(=O)C2=NOC(=C2)C3=CC=CS3

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Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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