IWP-2

Catalog No.S7085 Batch:S708505

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Technical Data

Formula

C22H18N4O2S3
Molecular Weight 466.6 CAS No. 686770-61-6
Solubility (25°C)* In vitro DMF (warmed with 50ºC water bath) 20 mg/mL (42.86 mM)
DMSO (warmed with 50ºC water bath) 3 mg/mL (6.42 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Homogeneous suspension for Oral and Intraperitoneal Injection. Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses. IWP-2 specifically inhibits CK1δ.
Targets
Wnt [1]
(Cell-free assay)		 M82FCK1δ [3]
(Cell-free assay)
27 nM 40 nM
In vitro

IWP-2 is useful in both regenerative medicine and anticancer efforts. IWP-2 inactivates Porcn, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. IWP-2 blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. [1]
Features Selective inhibitor of Porcn-mediated Wnt secretion.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    L-Wnt-STF cells

  • Concentrations

    5 μM

  • Incubation Time

    24 h

  • Method

    L-Wnt-STF cells that exhibit constitutive Wnt pathway activation were incubated with IWR (10μM) and IWP (5μM) compounds for 24 hrs prior to lysis. Cellular lysates were subjected to Western blot analysis.
Animal Study:

[2]
  • Animal Models

    Crl:CD1 (ICR) and FVB/N female mice

  • Dosages

    50 µg

  • Administration

    s.c.

Customer Product Validation

Data from [ , , Sci Rep, 2016, 6:32770. ]

Data from [ , , Methods, 2016, 101:103-12. ]

Data from [ , , Biochem Biophys Res Commun, 2017, 486(1):49-56 ]

Selleck's IWP-2 has been cited by 139 publications

ETVs dictate hPSC differentiation by tuning biophysical properties [ Nat Commun, 2025, 16(1):1999] PubMed: 40011454
Deciphering signaling mechanisms and developmental dynamics in extraembryonic mesoderm specification from hESCs [ Nat Commun, 2025, 16(1):4688] PubMed: 40399284
AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain [ Nat Commun, 2025, 16(1):3694] PubMed: 40251179
The balance between IFN-γ and ERK/MAPK signaling activities ensures lifelong maintenance of intestinal stem cells [ Cell Rep, 2025, S2211-1247(25)00057-9] PubMed: 39952238
Human epicardial organoids from pluripotent stem cells resemble fetal stage with potential cardiomyocyte- transdifferentiation [ Cell Biosci, 2025, 15(1):4] PubMed: 39825425
Heightened sensitivity to adverse effects of metformin in mtDNA mutant patient cells [ Life Sci, 2025, 366-367:123486] PubMed: 39978587
Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging [ Sci Rep, 2025, 15(1):2536] PubMed: 39833280
Interplay of SHH, WNT and BMP4 signaling regulates the development of the lamina propria in the murine ureter [ Development, 2025, 152(3)DEV204214] PubMed: 39817691
Restoration of excitation/inhibition balance enhances neuronal signal-to-noise ratio and rescues social deficits in autism-associated Scn2a -deficiency [ bioRxiv, 2025, 2025.03.04.641498] PubMed: 40093153
Human cerebral organoids model tumor infiltration and migration supported by astrocytes in an autologous setting [ bioRxiv, 2025, 2025.01.29.635456] PubMed: 39974912

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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