Forskolin

T24 cells were treated with IFN-α (1 × 10⁴ U/mL), TPL2i (2 μM), PD98059 (40 μM), or forskolin (50 μM) for 24 h. The expression levels of COX-2, CREB, and p-CREB were analyzed by western blotting. The β-tubulin was used as the loading control.

J Exp Clin Cancer Res, 2019, 37(1):288. Forskolin purchased from Selleck.

Western blot analysis shows pERK levels after 8 hours treatment with forskolin. AC activation with 10 μmol/L forskolin (F).

Mol Cancer Res, 2017, 15(12):1792-1802. Forskolin purchased from Selleck.

The pH-dependent regulation of the nucleo-cytoplasmic translocation of YAP and phosphorylation of YAP was regulated by Forskolin and H 89 2HCl, as assessed by western blotting.

Sci Rep, 2016, 6:26835. Forskolin purchased from Selleck.

Measurement of cAMP accumulation in INS-1 cells. (A) INS-1, or (B) INS-1 transfected with murine GPR109A for 24 h, or (C) INS-1 transfected with human GPR109A for 24 h were incubated in DMEM (2.5 mM glucose) media with or without PTX (100 ng/ml) for 24 h. Cells were then incubated in KRB supplemented with 2.5 mM glucose, 500 μM IBMX, 10 μM forskolin, and with/without 10 or 100 μM NA for 1 h. After lysis with HCl (0.1 M), cells were sonicated and spun. The supernatants were saved for cAMP measurement by ELISA. (D) Reduction of cAMP accumulation in INS-1 and INS-1 cells transfected with GPR109A in the presence of 10 or 100 μM NA. White bar: INS-1 cells, grey bar: INS-1 cells transfected with murine GPR109A; black bar: INS-1 cells transfected with human GPR109A. (∗ and #p < 0.05, ∗ and ##p < 0.001). (n = 5).

Gen Comp Endocrinol, 2016, 237:98-108. Forskolin purchased from Selleck.