Forskolin

Catalog No.S2449 Synonyms: Coleonol

Molecular Weight(MW): 410.5

Forskolin is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP in the study and research of cell physiology.

Cited by 18 Publications

Am J Hum Genet, 2018, 102(6):1062-1077

PubMed: 29805046 ( click the link to review the publication )

Eur J Cancer, 2014, 50(7):1310-20

PubMed: 24559688 ( click the link to review the publication )

Development, 2016, 143(12):2103-10

PubMed: 27151951 ( click the link to review the publication )

Mol Cancer Res, 2017, 15(12):1792-1802

PubMed: 28851815 ( click the link to review the publication )

Gen Comp Endocrinol, 2016, 237:98-108

PubMed: 27570060 ( click the link to review the publication )

Reprod Domest Anim, 2018, 10.1111/rda.13304

PubMed: 30098220 ( click the link to review the publication )

Front Pharmacol, 2018, 9:1176

PubMed: 30416443 ( click the link to review the publication )

Clin Cancer Res, 2018, 10.1158/1078-0432.CCR-18-2912

PubMed: 30504424 ( click the link to review the publication )

Sci China Life Sci, 2018, 10.1007/s11427-018-9359-8

PubMed: 30159682 ( click the link to review the publication )

J Exp Clin Cancer Res, 2018, 37(1):288

PubMed: 30482227 ( click the link to review the publication )

Cell Physiol Biochem, 2018, 45(3):1023-1033

PubMed: 29439250 ( click the link to review the publication )

J Exp Clin Cancer Res, 2018, 37(1):114

PubMed: 29866132 ( click the link to review the publication )
Osteoarthritis Cartilage, 2017, 25(11):1868-1879

PubMed: 28716756 ( click the link to review the publication )

PLoS One, 2017, 12(3):e0173240

PubMed: 28253352 ( click the link to review the publication )

Sci Rep, 2016, 6:26835

PubMed: 27256071 ( click the link to review the publication )

Oncogene, 2016, 35(38):5070-7

PubMed: 26973244 ( click the link to review the publication )

J Biol Chem, 2015, 290(50):30030-41

PubMed: 26499801 ( click the link to review the publication )

Toxicol Appl Pharmacol, 2015, 289(1):109-16

PubMed: 26367767 ( click the link to review the publication )

3 Customer Reviews

Eur J Cancer 2014 50(7), 1310-20. Forskolin purchased from Selleck.

Western blot analysis shows pERK levels after 8 hours treatment with forskolin. AC activation with 10 μmol/L forskolin (F).

Mol Cancer Res, 2017, 15(12):1792-1802. Forskolin purchased from Selleck.
Measurement of cAMP accumulation in INS-1 cells. (A) INS-1, or (B) INS-1 transfected with murine GPR109A for 24 h, or (C) INS-1 transfected with human GPR109A for 24 h were incubated in DMEM (2.5 mM glucose) media with or without PTX (100 ng/ml) for 24 h. Cells were then incubated in KRB supplemented with 2.5 mM glucose, 500 μM IBMX, 10 μM forskolin, and with/without 10 or 100 μM NA for 1 h. After lysis with HCl (0.1 M), cells were sonicated and spun. The supernatants were saved for cAMP measurement by ELISA. (D) Reduction of cAMP accumulation in INS-1 and INS-1 cells transfected with GPR109A in the presence of 10 or 100 μM NA. White bar: INS-1 cells, grey bar: INS-1 cells transfected with murine GPR109A; black bar: INS-1 cells transfected with human GPR109A. (∗ and #p < 0.05, ∗ and ##p < 0.001). (n = 5).

Gen Comp Endocrinol, 2016, 237:98-108. Forskolin purchased from Selleck.

Purity & Quality Control

Choose Selective cAMP Inhibitors

Biological Activity

Description

Forskolin is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP in the study and research of cell physiology.

Targets

Adenylyl cyclase (AC) ^[1]
(A wide variety of cell types)

In vitro

Forskolin increases cAMP levels in preparations of membranes, cells, or tissues. Forskolin not only activates AC but also interacts with certain other proteins, including glucose transporters and ion channels. Forskolin is able to promote activation of nine different transmembrane iso-forms of AC, albeit with somewhat less efficacy for AC9, which could be used to provides a means to identify and quantify high-affinity binding sites, i.e., G-proteins (G_s)–AC complexes. Activation of _s by GPCRs contributes to Forskolin-stimulated cAMP generation in cells because of _s-Forskolin potentiation of AC activity. ^[1] Forskolin stimulates adenylate cyclase activity without interacting with cell surface receptors. Forskolin's potentiation of cAMP in turn inhibits basophil and mast cell degranulation and histamine release, lowers blood pressure and intraocular pressure, inhibits platelet aggregation, promotes vasodilation, bronchodilation, and thyroid hormone secretion, and stimulates lipolysis in fat cells. Forskolin inhibits the binding of platelet-activating factor (PAF), independently of cAMP formation, which may be a result of Forskolin's direct effect on PAF or via interference with PAF binding to receptor sites. Forskolin also appears to have an effect on several membrane transport proteins, and inhibits glucose transport in erythrocytes, adipocytes, platelets, and other cells. Forskolin is used to treat with glaucoma. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
HEK293	NUKxZ3hFTnWwY4Tpc44hSXO\|YYm=	MmL0NVAh\|ryP	NUDxdmxUPiCq		MUTpcoNz\WG\|ZYOgdIhwe3Cqb4L5cIF1cW:wIH;mJI93\XKneIDy[ZN{\WRiS1zIUFMh[XRiU{SzNy=>	NGTJNZAzPjR\|NUS5PC=>
Mo-DCs	M4m0XmZ2dmO2aX;uJGF{e2G7	M4\DdlUxKM7:TR?=	MX:yOQKBkWh?		Mkj2dJJwdW:2ZYOgTWwuOjNicILv[JVkfGmxbjDpckB1cGVic4Xw[ZJv[XSjboSgc4Yhgnmvb4PhckB{fGmvdXzheIVlKE2xLVTDd:Kh	NXfpXmJbOjZ2MUK5OFg>
RBMECs	NFWydI9HfW6ldHnvckBCe3OjeR?=	M1vGb\|XDqM7:TR?=	NULBRXg5OcLiaB?=		NUHKXXhP[myxY3vzJJRp\SCUYXOxJIlv[WO2aY\heIlwdiCrbnT1Z4VlKGK7IFXNRXAuUUl?	MmXmNlY{PThyM{m=
INA-6	NXTmRlJ1S2WubDDWbYFjcWyrdImgRZN{[Xl?	M2HyN\|AuOTByIN88US=>	MmSzO\|LjiImq		NFXJZYZqdmS3Y3XzJINmdGxiZHXheIgh\G:\|ZTDk[ZBmdmSnboTsfS=>	M3LuR\|I3OzB4NkK0
OPM-2	M{jpSGNmdGxiVnnhZoltcXS7IFHzd4F6	MlnrNE0yODBizszN	NFfyemc4OuLCiXi=		MXzpcoR2[2W\|IHPlcIwh\GWjdHig[I9{\SCmZYDlcoRmdnSueR?=	MVOyOlMxPjZ{NB?=
U266	NF\QUlNE\WyuIG\pZYJqdGm2eTDBd5NigQ>?	NF\2c20xNTFyMDFOwG0>	NXz0ZWc{PzMkgJno		NHLRd2tqdmS3Y3XzJINmdGxiZHXheIgh\G:\|ZTDk[ZBmdmSnboTsfS=>	M3TpblI3OzB4NkK0
H929	MX3D[YxtKF[rYXLpcIl1gSCDc4PhfS=>	NHXzNooxNTFyMDFOwG0>	NHXyWVg4OuLCiXi=		M3vKUYlv\HWlZYOgZ4VtdCCmZXH0bEBld3OnIHTldIVv\GWwdHz5	MknBNlY{ODZ4MkS=
RPMI 8226	M4jNZWNmdGxiVnnhZoltcXS7IFHzd4F6	Ml3YNE0yODBizszN	NXTzbopFPzMkgJno		MmnObY5lfWOnczDj[YxtKGSnYYToJIRwe2ViZHXw[Y5l\W62bIm=	MmX5NlY{ODZ4MkS=
MDCK	MmPMSpVv[3Srb36gRZN{[Xl?	MkfINVAhyrWP	NFnn[4gzPCCq	MkTTSG1UVw>?	M1jEZZVxemWpdXzheIV{KHSqZTDlfJBz\XO\|aX;uJI9nKFSJRj5OtlEh[W6mIFPUS2bDqA>?	MmD4NlYzODJ\|NUK=
MDCK	NHzuWY1HfW6ldHnvckBCe3OjeR?=	NUPKNWRkOTBiwsXN	NF;Fb3IzPCCq	M4i3dGROW09?	MW\pcohq[mm2czD0bIUhcW6lcnXhd4VlKGW6cILld5Nqd25ib3[gSm4h[2G3c3XkJIJ6KFSJRj5OtlE>	Ml;CNlYzODJ\|NUK=
Spinal cords	MVPGeY5kfGmxbjDBd5NigQ>?	NWnZ[lNLOcLizszN	Mn\XN\|AhdWmw		NFuwPWl{fGmvdXzheIV{KGODTWCgcIV3\Wy\|	M1yzdlI3OTJ4OUK2
BeWo	MnXOSpVv[3Srb36gRZN{[Xl?	MXqyOgKBkc7:TR?=	Mn61NlQwPDhxN{KgbC=>		NXHzNHpWdGWjZIOgeI8h[W5iaX7jdoVie2ViaX6geIhmKGW6cILld5Nqd25ib3[gc5Rp\XJiZoXzbY9vKG2jcnvldpM>	M1[0flI3ODV\|NUS5
bovine oocytes	M4q2PWZ2dmO2aX;uJGF{e2G7	MWqxNFDDqM7:TR?=	MXWxNuKhcA>?		NY[0c\|J5cW6qaXLpeJMhfGinIHXm[oVkfCCxZjDOVHBCKGGwZD;vdkBPWFCFIITvJJN1cW23bHH0[UBz\XO3bYD0bY9vKG:oIH3lbY9{cXN?	MmPZNlYxPTF4MUG=
Caco-2	M1zPcmZ2dmO2aX;uJGF{e2G7	M3;aZlAvOS9zL{GwJO69VQ>?	MmPtNlDDqG2rbh?=		NHL6OItqdmS3Y3XzJIEh\G:\|ZT3k[ZBmdmSnboSgbY5kemWjc3WgbY4hcW62cnHj[YxtfWyjcjDjRW1RKGyndnXsdy=>	MUOyOlA1QTFyMh?=
Caco-2	NGP1SG1HfW6ldHnvckBCe3OjeR?=	NWfkdnhxOC5zL{GvNVAh\|ryP	Mnj2NlQhcA>?		M2LNRolv[3KnYYPld{BOWlB{IIDyc5RmcW5ibHX2[Yw>	M2LnNVI3ODR7MUCy
AML-12	M{DIfWZ2dmO2aX;uJGF{e2G7	NIDFWowzOCEQvF2=	NGH0RWo{KGh?		Mkf5eZBz\We3bHH0[ZMhfGinIIDoc5NxcG:{eXzheIlwdiCuZY\lcJMh[XRiVHjyMVQyOSCjbnSgV4VzNTR7Mx?=	MkHpNlYxPDh7OEW=
AML-12	M1fQUmZ2dmO2aX;uJGF{e2G7	MXOyNEDPxE1?	NV35[XB5OS16IHi=		NX63fFkxcW6lcnXhd4V{KGeudXPvd4UheHKxZIXjeIlwdg>?	MmnZNlYxPDh7OEW=
AML-12	MVPGeY5kfGmxbjDBd5NigQ>?	NGXmeVgzOCEQvF2=	M2PPO\|MhcA>?		M1:5fpVxNXKnZ4XsZZRme8LiUHfjNYEtyqCSZYDjb{wh[W6mwrDHOpBkyqCvUl7BJIxmfmWucx?=	NWX1V41UOjZyNEi5PFU>
AML-12	NU\OV5VuTnWwY4Tpc44hSXO\|YYm=	NYrofIZFOjBizszN	NFXGb4c{KGh?		MXLpcoR2[2W\|IITo[UBl\XCqb4PwbI9zgWyjdHnvckBw\iCFUmTDNuKh	MmnhNlYxPDh7OEW=
RBMECs	MXHGeY5kfGmxbjDBd5NigQ>?	MnTYOeKh\|ryP	NFHETYkyKGh?		MmfabY5pcWKrdIOgSW1CWC2LST3pcoR2[2WmIHnuZYN1cX[jdHnvckBw\iCUYYCxxsA>	M{fyUVI3ODR2Nk[z
EndoC-βH1	NYnRNFJyTnWwY4Tpc44hSXO\|YYm=	MlrBOeKh\|ryP	MkXlNUBp		M3faNJBwfGWwdHnheIV{KGeudXPvd4UucW6mdXPl[EBqdnO3bHnuJJNm[3KndHnvckBqdiC2aHWgdJJme2WwY3Wgc4Yh\2y3Y3;z[S=>	M2jHWlI3ODJ6NU[y
EndoC-βH1	NETUVmpHfW6ldHnvckBCe3OjeR?=	MoiyOeKh\|ryP	M2LiOFEhcA>?		NH;uOJRt\WGmczD0c{BiKHO2cn;u[{BkSU2SIHnuZ5Jm[XOn	MomzNlYxOjh3NkK=
SH-SY5Y	NXTKPWFOTnWwY4Tpc44hSXO\|YYm=	MlvtN\|Ah\|ryP	MkfZN\|AhdWmw	M3XRdGROW09?	NHTsR2h{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczD0bIUh[WO2aY\heIlwdiCxZjDQT2E>	MWGyOlAzPTF\|Nx?=
PC-3	M3;yR2Z2dmO2aX;uJGF{e2G7	NX\QTG9OPDBiwsXN	Ml7mNkBp	MUTEUXNQ	NF;BcWRt\WGmczD0c{BRWDKDIHHjeIl3[XSrb36=	MoPUNlYxOjN6M{[=
PC-3	MljqR4VtdCCYaXHibYxqfHliQYPzZZk>	MX60NEDDvU1?	M2q0WlI1NzR6L{eyJIg>	NFfoOnNFVVOR	Mlzp[IVkemWjc3XzJINmdGxidnnhZoltcXS7IITpcYUh\GWyZX7k[Y51dHl?	MUWyOlAzOzh\|Nh?=
SCG	M{P1fmZ2dmO2aX;uJGF{e2G7	M3\XUVIxKM7:TdMg		MXfEUXNQ	M4\adJJmfmW{c3nicJkhe3WycILld5NmeyCLS2[ge4l1cCCjIFnDOVAhd2ZiMkSuOEDPxE1?	MXmyOVk3OjF\|Mh?=
HEK-293	MnjYSpVv[3Srb36gRZN{[Xl?	M163R\|M2KM7:TdMg		M3zBNmROW09?	MYfpcoR2[2W\|IHGgZ49ve3CrY4XveZMh6oDeaX7hZ5RqfmG2aX;u5qCeKG:oIITo[UBMfjJwMTDjeZJz\W62	M{TxXFI2QTZ{MUOy
SCG	NGD5WZRHfW6ldHnvckBCe3OjeR?=	M1;mfVExOCEQvF5CpC=>		Ml2ySG1UVw>?	MXny[YR2[2W\|IITo[UBmgGOrdHHibYxqfHlib3[gV2NIKG6ndYLvcpM>	M2nMd\|I2QTZ{MUOy
PCCL3	NGLTe4dHfW6ldHnvckBCe3OjeR?=	NH7pb5QyOCEEtV2=	MVmyOEBp		MXPlcohidmOnczDEeW95OiCycn;tc5RmeiC2cnHud4NzcXC2aX;uJIFkfGm4aYT5xsDjiIt?	NEDqUIQzPTl4MEm1Oi=>
3T3-L1 preadipocytes	NFX4b21HfW6ldHnvckBCe3OjeR?=	MnzyNVAh\|ryPwrC=	M1vQd\|EzKGh?		M2TXdYlv\HWlZYOgR3JGSiCyaH;zdIhwenmuYYTpc44h[W6mIFOvSWJR\|rJiZYjwdoV{e2mxbh?=	NV\QWGRWOjV7MkiwOVg>
H295R	NVPDVm1ZTnWwY4Tpc44hSXO\|YYm=	MkDTNVDDqM7:TR?=	MXK0POKhcA>?		NUTCNXB6cW6lcnXhd4V{KHO2ZYLvbYQhdWW2YXLvcIl1\XNiaX6geIhmKGGwZILv[4VvNCCvaX7ldoFtdy1iYX7kJIdtfWOxY3;yeIlkd2mmIIDheIh4[Xm\|	MofxNlU5Pjl3NU[=
PBMC	MVnGeY5kfGmxbjDBd5NigQ>?	NH:wPGI2OMLizszN	MXmyOOKhcMLi		NWLFV\|JqcW6qaXLpeJMhfGinIHnuZ5Jm[XOnZDDz[YNz\XSrb36gc4YhXE6IIHnu[JVk\WRiYomgeIhmKESSRR?=	NIjOUW4zPTh4NkC3PS=>
GLUTag	M3;MZmZ2dmO2aX;uJGF{e2G7	M1H2OVEx6oDLwsXN	MoPxNE8zNzRiaB?=		M3TkO5N1cW23bHH0[ZMhT0ySLUGgd4VkemW2aX;uJINwfHKnYYTl[EB4cXSqIFnCUXg>	NVHCbYk2OjV6M{K2N\|E>
GLUTag	MlfPSpVv[3Srb36gRZN{[Xl?	M17XW\|Ex6oDLwsXN	NHnpeI01KGh?		MUTpcoNz\WG\|ZYOgeIhmKHCFUlXCJIxmfmWuczD3bZRpKHSqZTDJRm1Z	MU[yOVg{OjZ\|MR?=
BAECs	MUXGeY5kfGmxbjDBd5NigQ>?	NHrkN40zPSEQvF2=	NHjlWHozPCCq		NG[2ZpFmdmijbnPld{B1cGViYXP0bZZifGmxbjDv[kBRWEGUzsGgZpkhPSEQvF2gdoV{fmW{YYTyc4wtKFR2SGOsJI9zKDRvUFHQ	NXP5[IpxOjV5OUi4NlY>
PC12	M3fF[GZ2dmO2aX;uJGF{e2G7	MlPQNlXDqM7:TR?=	NXnZemxIPDhiaB?=		NVn4TnlL[WO2aY\heIV{KGODTWC=	MmfDNlU4Pjl\|MEW=
GH3	NITNeG1HfW6ldHnvckBCe3OjeR?=	M1zwXVHDqM7:TR?=	MW[2MYg>		NI\iTVdifHSnboXheIV{KHSqZTDjc5Jz\WyjdHnvckBj\XS5ZXXuJHBTVCCjbnSgRo1idDFiZYjwdoV{e2mxbh?=	MXOyOVczPzBzOB?=
GH3	NH;1TZRHfW6ldHnvckBCe3OjeR?=	M{njXVHDqM7:TR?=	MoHCOk1p		M4\aWIlv\HWlZYOgVHJNKGGwZDDCcYFtOSxiYoX0JI5wfCCFbH;jb{whdVKQQTDlfJBz\XO\|aX;u	NF3LWVUzPTd{N{CxPC=>
BeWo	M3\Tc2Z2dmO2aX;uJGF{e2G7	NHfkVmkyOOLCit88UeKh	Ml3mO\|IhcA>?	M1u4dmROW09?	NVz0RXFKdWWmaXH0[ZMhSmWZbzDj[YxtKGSrZn\ldoVvfGmjdHnvci=>	M4K0bFI2PzF\|NEK1
oocytes	NWLIdlFCTnWwY4Tpc44hSXO\|YYm=	Mo\lOUDPxE1?	MVOyOEBp		Mmq1ZZR1\W63YYTld{BzcC2rboP1cIlvKGGldHnvckBwdiCxb3P5eIUhT1[ERDDzbYdvcW[rY3HueIx6yqB?	NGTRU\|MzPTdyN{i1OC=>
SC	NXzjXnBiTnWwY4Tpc44hSXO\|YYm=	NFO1dJkxNjVizszN	M3nCN\|I1KGh?		NFy4T4lucW2rY3vzJJRp\SCnZn\lZ5Qhd2ZiY1HNVEBidmGub3fzJI9vKE9zIHHu[EBOSlBiZYjwdoV{e2mxbh?=	MkX3NlU4ODV6N{S=
SC	NYrhV45HTnWwY4Tpc44hSXO\|YYm=	MWWwMlUh\|ryP	M4fqSFczKGh?		NVTpPIxPcW6lcnXhd4V{KGKxdHigT5JwgC1{MDDhcoQhVzFiZYjwdoV{e2mxbjDpckBigG:wLYLlcIF1\WRiU1PzJIJ2fCCxbnz5JGtzd3hvMkFCpC=>	MUKyOVcxPTh5NB?=
UACC-647	Ml7FSpVv[3Srb36gRZN{[Xl?			NXnvUIZ6TE2VTx?=	NUjyTJp3dGWjZIOgeI8h[SC{aYPlJIlvKGODTWCgcIV3\Wy\|IDjFR\|UxyqB;wrCyNE4{QcLizszNLS=>	MoriNlU4ODNyMkW=
UACC-647	NGT1[mJHfW6ldHnvckBCe3OjeR?=	NY[yemgzOTEEoN88US=>	NWHIRYtpOTVibXnu	M1HMSWROW09?	NHK1SndqdmirYnn0d{BGWkticHjvd5Bpd3K7bHH0bY9v	M2DXNVI2PzB\|MEK1
UACC-647	M1fDbmZ2dmO2aX;uJGF{e2G7	M2fuTFExyqEQvF2=	MoO3NVUhdWmw	NEfEO3VFVVOR	MXrpcoNz\WG\|ZYOg[WVHOiCyaH;zdIhwenmuYYTpc44hdGW4ZXzzxsA>	MYWyOVcxOzB{NR?=
SH-SY5Y	NIiye25HfW6ldHnvckBCe3OjeR?=	MVWxNOKh\|ryP	M4S0RlHDqGkEoB?=		M2Tte4lv[3KnYYPld{BNXUNiYXP0bZZqfHl?	NYmxXmtKOjV3OUe0N\|M>
SH-SY5Y	NFnFXJNHfW6ldHnvckBCe3OjeR?=	MX2xNOKh\|ryP	NWTafmo3OcLiaNMg		NELIbWVqdmO{ZXHz[ZMhSUeFMTDtVm5CKGyndnXs	NGDBfVQzPTV7N{SzNy=>
hADSCs	M3npNWZ2dmO2aX;uJGF{e2G7	MkXJOgKBkcL3TR?=	NGX6fHE{OCCvaX6=		MlzFbY5kemWjc3XzJINCVVBibHX2[Yx{	NHWzWnAzPTV7MUmwPC=>
HEK293	NWXjTYJ3TnWwY4Tpc44hSXO\|YYm=	NXm2XG06PeLCidM1US=>	MkHMN\|AhdWmw		MoW5bY5kemWjc3XzJINCVVBibHX2[Yx{	NHTNTYkzPTV7MUmwPC=>
3T3-L1	M3r6NmZ2dmO2aX;uJGF{e2G7	NIW3S\|gzNjVxNTFOwG0>	M3jRdFI1KGkEoB?=		NYKwVJV4e2mpbnnmbYNidnSueTDk[YNz\WG\|ZYOgRXRIVCCycn;0[YlvKGW6cILld5Nqd25iYYSgZYxtKGSxc3XzJJRme3SnZB?=	NVH2VXA6OjV3OUC1PVc>
OCI-Ly1	MkPFSpVv[3Srb36gRZN{[Xl?	M1nUTFQxyqEQvF2=	NWjtcYJOOcLiaNMg	NVrBXHZyTE2VTx?=	MmTLbY5lfWOnczD0bIUhcW6lcnXt[Y51KG:oIHPBUXAh[2:wY3XueJJifGmxboO=	MlewNlU2PzZ{MkC=
OCI-Ly18	M3HORmZ2dmO2aX;uJGF{e2G7	MknPOFDDqM7:TR?=	Mnv3NeKhcMLi	NF;1W4RFVVOR	NHnU[IFqdmS3Y3XzJJRp\SCrbnPy[Y1mdnRib3[gZ2FOWCClb37j[Y51emG2aX;udy=>	MmWzNlU2PzZ{MkC=
BeWo	MXzGeY5kfGmxbjDBd5NigQ>?	NEC2VHgzOMLiwsXN	MUO0POKhcA>?	MUDEUXNQ	MmC0bY5kemWjc3XzJJRp\SCmaX\m[ZJmdnSrYYTpc44hd2ZiQnXXc{Bk\Wyucx?=	NIHuc3MzPTV4Nke0NC=>
BeWo	NHjkfJdHfW6ldHnvckBCe3OjeR?=	MUSyNOKhyrWP	M3jOfFQ5yqCq	MXHEUXNQ	Mn35bY5kemWjc3XzJJRp\SCjZHjld4lwdiCxZjDUTHAuOSCvb37vZ5l1\XN?	MYWyOVU3Pjd2MB?=
LNCaP	MYLGeY5kfGmxbjDBd5NigQ>?	M1ToSVExyqEQvF2=	MlXqNVIhcMLi	Mlz2SG1UVw>?	MmTXbY5lfWOnczDhJIRz[W2jdHnjJIlv[3KnYYPlJI9nKEOURVKxJIFkfGm4aYT5	NWnLbIJ1OjV3NEiwPVk>
ThGCs	NX\kTYFwTnWwY4Tpc44hSXO\|YYm=	MlXRNVDjiIsQvF2=	NFrBT5E16oDMaB?=		MX\heYdu\W62czDITWYySSCuZY\lcJMhfGijdDD3[ZJmKHO2aX31cIF1\WRiYomgR49EdDJ?	NFzYWIEzPTR\|M{CyOy=>
ThGCs	MWXGeY5kfGmxbjDBd5NigQ>?	M3LDPVEx6oDMzszN	NHzhVoU16oDMaB?=		NGHYcIdqdmO{ZXHz[ZMhS2:FbEKtbY5lfWOnZDDFSG4zyqCpZX7lJIV5eHKnc4Ppc44>	MoXmNlU1OzNyMke=
ThGCs	NGHQeJlHfW6ldHnvckBCe3OjeR?=	MVmxNQKBks7:TR?=	NWrYTpVtOyCq		NGmzXolqdmirYnn0d{B1cGViZX\m[YN1KG:oIFiyU\|Ihd25iRVTONkBuWk6D	MnzENlU1OzNyMke=
RBMECs	M4rUU2Z2dmO2aX;uJGF{e2G7	MmDxNE4xPS9yLkWvOUDPxE1?	MoHGNE4zPSCq		MWjpcoNz\WG\|ZYOgZ2FOWCClb37j[Y51emG2aX;u	M4PIRlI2PDF4NkWx
RBMECs	MUDGeY5kfGmxbjDBd5NigQ>?	NI\0SJI2yqEQvF2=	MUSxJIg>		NUn4W5V7[myxY3vzJJRp\SCjY4TpeoF1cW:wIH;mJHJpd0FxUl;DT{BqdmS3Y3XkJIJ6KEWPQWCtTWk>	M1XnW\|I2PDF4NkWx
RBMECs	MXTGeY5kfGmxbjDBd5NigQ>?	NU[xOHhPPcLizszN	MXqxJIg>		MXLwdoV3\W62czD0bIUhTU2DUD3JTU1qdmS3Y3XkJHRGTVJidnHseYUh\GWlcnXhd4U>	M3TLTFI2PDF4NkWx
RBMECs	NXm1emI6TnWwY4Tpc44hSXO\|YYm=	NXjEV2UzPcLizszN	NGjVbFkyKGh?		M2nWSJBz\X[nboTzJJRp\SCrbnPy[YF{\SCrbjDIVnAh\my3eDDhZ5Jwe3NidHjlJGJVSiCrbnT1Z4VlKGK7IDDFUWFRNUmL	MnnINlU1OTZ4NUG=
RBMECs	NYL0RYpxTnWwY4Tpc44hSXO\|YYm=	Mk\3OeKh\|ryP	NVPhXJdOOSCq		MVfpcohq[mm2czD0bIUh\GWlcnXhd4VlKG:oIHHtc5VvfCCxZjDaU{0yKGmwIF3Gd{BqdmS3Y3XkJIJ6KEWPQWCtTWk>	NG[yfFQzPTRzNk[1NS=>
RBMECs	MoHrSpVv[3Srb36gRZN{[Xl?	M3zW[FXDqM7:TR?=	MW[xJIg>		NHTzOFhz\X[ncoPld{B1cGViY3jhcodmeyCrbjDaU{0yKGSrc4TybYJ2fGmxbjDz[YVvKHerdHigSW1CWC2LSTD0doVifG2nboS=	M2jOflI2PDF4NkWx
RBMECs	Mm\pSpVv[3Srb36gRZN{[Xl?	NVW0Rmo{PcLizszN	NX7HT3ZJOSCq		M2DjO4Jtd2OtczD0bIUhTU2DUD3JTU1qdmS3Y3XkJINp[W6pZTDpckBOVENicHjvd5Bpd3K7bHH0bY9v	Mn7INlU1OTZ4NUG=
RBMECs	M{DwZmZ2dmO2aX;uJGF{e2G7	MoXHOeKh\|ryP	NIDKPFEyKGh?		Ml7IZoxw[2u\|IITo[UBi[3SrbjDjfZRwe2unbHX0c44hemWjcoLhcodmdWWwdDDz[YVvKHerdHigSW1CWC2LSTD0doVifG2nboVCpC=>	NUm4NGNSOjV2MU[2OVE>
Primary bovine chondrocytes	NISye3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NVS3TXNyPc7:TR?=	NV\IV\|NzPDhiaB?=		M1n1UJJmfmW{c3XzJJRp\SCrbnjpZol1d3K7IHXm[oVkfCCxZjDj[Yxm[2:6aXKgc44heHKxbHnm[ZJifGmxbjDpckBoem:5dHigdIxifGViY3jvcoRzd2O7dHXz	M3rwPFI2PDB4MEG2
EM1	NELUR4lHfW6ldHnvckBCe3OjeR?=	MXmxOgKBks7:TR?=	MUO0PEBp		NHfkdHZz\WS3Y3XzJJRp\SCneIDy[ZN{cW:wIH;mxsBNUUcEoH;yxsBRXEeVMtMgbY7DqEODTGKtJI9zyqCHUFHDNk1{cWynbnPl[EBGVTFiY3XscJPDqA>?	MU[yOVM4QDZ4MR?=
BeWo	NGPRZlRHfW6ldHnvckBCe3OjeR?=	MkHBNlDDqML3TR?=	NHqwRYc1QMLiaNMg	NHj6dYdFVVORwrC=	NIGzfZNqdmO{ZXHz[ZMhfGinIHLleIEucEOJIILlcIVie2V?	M4nWfFI2OzZ{Mk[w
BeWo	NGjNVYdHfW6ldHnvckBCe3OjeR?=	Moj1NlDDqML3TR?=	NHT6UJM1QMLiaNMg	M1yzS2ROW00EoB?=	NYnkdYM5\G:5boLl[5Vt[XSnczD0bIUhdGW4ZXygc4YhXE2HTV[xOi=>	NGKxepAzPTN4MkK2NC=>
BeWo	MWDGeY5kfGmxbjDBd5NigQ>?	MkD3NlDDqML3TR?=	MVG0POKhcMLi	MmLmSG1UV8Li	NI[xRnlld3ewcnXneYxifGW\|IITo[UBt\X[nbDDv[kBIS01vMR?=	M2PPUlI2OzZ{Mk[w
granulosa cells	NFq0OllHfW6ldHnvckBCe3OjeR?=	MYGxNEDPxE1?	MUWxNk8zPCCq		MlzhbY5kemWjc3XzJJRp\SCuZY\lcJMhd2cEoGLHV\|LDqG2UTlG=	Mmi3NlU{OzlzMEW=
granulosa cells	NGO2SJdHfW6ldHnvckBCe3OjeR?=	NXPz[mlpOTBizszN	MknFNVIwOjRiaB?=		NF:wdHZqdmO{ZXHz[ZMhfGinIHzleoVteyCxZjDy[ZBwenSncjDhZ5Rqfmm2eTDmc5IhfGinIHzvcodme3RiZoLh[41mdnRiKPMIllg2PC9tMUjSS3MzNkyXQzm=	MkTPNlU{OzlzMEW=
granulosa cells	NILVSXVHfW6ldHnvckBCe3OjeR?=	MUixNEDPxE1?	M2DHUVI1KGh?		MV;pcoNz\WG\|ZYOgeIhmKGmwdHXud4l1gSCxZjDEUmEweHKxdHXpckBkd22ybHX4	NYX3RYhOOjV\|M{mxNFU>
granulosa cells	NHTaUXlHfW6ldHnvckBCe3OjeR?=	NYD0W4ZGOTBizszN	MVOyOEBp		NHHWXo1qdmO{ZXHz[ZMhfGinIHzleoVteyCxZjDSS3MzKHC{b33veIVzKGGldHn2bZR6	NVnLWpRDOjV\|M{mxNFU>
SK-N-AS	NWP6UlFFS2WubDDWbYFjcWyrdImgRZN{[Xl?	MoPiNVAh\|ryPwrC=	M1H3UVI1NzR6IHi=		M4PvbIVvcGGwY3XzJJRqdWVvZHXw[Y5l\W62bImgZ4VtdHWuYYKgeoli[mmuaYT5xsA>	NHzxOXQzPTJ4NkC2Ny=>
SK-N-AS	MkXTSpVv[3Srb36gRZN{[Xl?	M4nl[\|ExKM7:TdMg	NIi1SGIzPCCq		MUnpcoNz\WG\|ZYOgeIhmKGODTWCgcIV3\Wy\|wrC=	MWOyOVI3PjB4Mx?=
SK-N-AS	NV\memwyTnWwY4Tpc44hSXO\|YYm=	M{LkZ\|ExKM7:TdMg	MmryNlQhcA>?		MlnVbY5kemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJIN6[2yrbjDENS=>	MmWxNlUzPjZyNkO=
SK-N-AS	Mmn4SpVv[3Srb36gRZN{[Xl?	NHe1TXAyOCEQvF5CpC=>	NFjxepE{OCCvaX6=		NGrUcI1qdmS3Y3XzJJBpd3OyaH;yfYxifGmxbjDv[kDPui2lYYTlcolvKCi\|ZYK2O\|UqNCCyLVfTT\|PPuiBqc3XyPUkh[W6mIHPvcoNwdWm2YX70JIhq\2incjDs[ZZmdHNib3[gZYN1cX[nLDD1cpBpd3OyaH;yfYxifGWmLDFOtk1k[XSnbnnu	MnTaNlUzPjZyNkO=
SK-N-AS	NX\r[GhDTnWwY4Tpc44hSXO\|YYm=	MVGxNEDPxE4EoB?=	NVflO5JpOTBxM{CvOlAhdWmw		MWPpcoNz\WG\|ZYOgcIV3\Wy\|IH;mJJAu\|rJvY3H0[Y5qdiBqc3XyOlc2MSCjbnSgbY5lfWOnczDhZ4N2dXWuYYTpc44hd2ZicD5Otk1k[XSnbnnuJEh{\XJ4N{WpJIlvKCiyZYLpLY52[2ynYYKgdoVocW:wcx?=	MnvBNlUzPjZyNkO=
SK-N-SH	M{P1O2NmdGxiVnnhZoltcXS7IFHzd4F6	NHW0[JAyOCEQvF5CpC=>	MXy0PEBp		NYfpfo57\W6qYX7j[ZMhW0tvTj3TTEBv\XW{b3LsZZN1d22jIHPlcIwhfmmjYnnsbZR6	NYL4TZRtOjV{Nk[wOlM>
HEK‐CFTR	NIfzN45HfW6ldHnvckBCe3OjeR?=	M{LqRVLjiJN3MNMg{txO	M{HvRVAuOTJibXnu	M1GxSGROW00EoB?=	Ml62bY5lfWOnczDhJIRwe2YkgKDk[ZBmdmSnboSgbY9lcWSnIHXm[ox2gMLi	M3Po[VI2OjZ\|MkC3
L6	M4rZeWZ2dmO2aX;uJGF{e2G7	NXLtfIlGPDBiwsXN	NFX1clUzPCCq		NFjyenlqdmirYnn0d{BFVUhzLXnu[JVk\WRiQXv0JIFkfGm4YYTpc44>	NYn4XpF3OjV{NEe1OVA>
MIN6	NV7KTHNGTnWwY4Tpc44hSXO\|YYm=	M2XBVVExKM7:TdMg	NGHLSVQ{KGh?		MmXSbY5kemWjc3XzJGQ{KG2UTlGg[ZhxemW\|c3nvci=>	NFXZbHQzPTJ2MUGyOC=>
ventricular cardiomyocytes	NFXuTGVHfW6ldHnvckBCe3OjeR?=	MnfFNE4xOS1zMDFOwG0>			NGfLNVNqdmO{ZXHz[ZMhKGODTWCgZYNkfW23bHH0bY9v	NILXWIczPTJyM{GxNy=>
ventricular cardiomyocytes	M{H3NGZ2dmO2aX;uJGF{e2G7	NUW2OYpUOC5yMT2xNEDPxE1?			NY\6dFlL\X[xa3XzJIFvKGmwb4Tyc5Bq[yC{ZYPwc45{\SBzMkFCtVE2LSCjYn;2[UBj[XOjbDD3bZRpKGGwIFXDOVDDqG:oIEKuNkDDvU1?	NF3JWFgzPTJyM{GxNy=>
BeWo	M3Ts[mZ2dmO2aX;uJGF{e2G7	NXfoSXV4OjBiwsXN	NIjvdI01QCCq	NELWfWRFVVORwrC=	NWTVUHBycW6mdXPld{Bk\WyuIH\1d4lwdg>?	MoHCNlUyQDR2N{e=
THP-1	MVnGeY5kfGmxbjDBd5NigQ>?	MVGxM\|ExKM7:TR?=	NELEXXczyqCq	NFfpdphFVVORwrC=	MnL5d5VxeHKnc4Pld{BOS1BvMTDwdo9lfWO2aX;uxsA>	MV[yOVE2PDh6Mh?=
Huh-7	NIS5UpJHfW6ldHnvckBCe3OjeR?=	NHjFSHgxNTJyIN88US=>	MmfGNkBpyqB?		NEjLZ\|dz\XO3bITzJIlvKGFiZH;z[U1l\XCnbnTlcpQhcW6lcnXhd4UhcW5iYz3NfYMh\XiycnXzd4lwdiCjdDD0bIUheHKxdHXpckBidmRibWLORUBt\X[nbIO=	Mn;0NlUyODl6M{S=
C6	MlvnSpVv[3Srb36gRZN{[Xl?	MlHGNVAh\|ryPwrC=	NGHkbnYzOCCvaX6=		MVHpcoNz\WG\|ZYOgZ2FOWCCjY3P1cZVt[XSrb36=	NUfNUYRROjVyNkm0NVc>
SW480	Mn3OSpVv[3Srb36gRZN{[Xl?	NXLZVG9iPDEEoN88US=>	MWe0POKhcA>?	MVPEUXNQ	MXjhZ5RqfmG2ZYOgVHAzSQ>?	MXmyOFk6PzR3MR?=
HT-29	MWDGeY5kfGmxbjDBd5NigQ>?	MnPROFDDqM7:TR?=	MUW0POKhcA>?	MWHEUXNQ	MojDZYN1cX[jdHXzJHBROkF?	M{fHT\|I1QTl5NEWx
SW480	M4PvZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MkWxOFDDqM7:TR?=	MV[wMVczKGh?	MnPQSG1UVw>?	MkLhbY5pcWKrdIOgZ4VtdCCpcn;3eIghfGmvZTDk[ZBmdmSnboTsfS=>	M3nvWFI1QTl5NEWx
HT-29	M1zONmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MXm0NOKh\|ryP	NHvZcosxNTd{IHi=	MlO4SG1UVw>?	MWjpcohq[mm2czDj[YxtKGe{b4f0bEB1cW2nIHTldIVv\GWwdHz5	M1HDdFI1QTl5NEWx
SW480	MWHGeY5kfGmxbjDBd5NigQ>?	NYLTU205PDEEoN88US=>	NV;Y[oVPPyCm	NH6wO5lFVVOR	MUfy[YR2[2W\|IHPvcI9vd3OyaHXy[UBnd3KvYYTpc44h[2GyYXLpcIl1gcLi	Moj1NlQ6QTd2NUG=
HT-29	MkPMSpVv[3Srb36gRZN{[Xl?	NFnIfHU1OMLizszN	NX7tRnQ3PyCm	NH74d\|NFVVOR	M2fTXZJm\HWlZYOgZ49td26xc4Do[ZJmKG[xcn3heIlwdiClYYDhZoltcXS7wrC=	NIPIdWYzPDl7N{S1NS=>
SW480	M4TYemFxd3C2b4Ppd{BCe3OjeR?=	MnjQOFDDqM7:TR?=	NVHoRWlxPDkEoHi=	MlrxSG1UVw>?	MkXIbY5lfWOnczDhckBi[3SrdnH0bY9vKG:oIHPhd5Bie2ViMz:3	MkHhNlQ6QTd2NUG=
HT-29	MUDBdI9xfG:\|aYOgRZN{[Xl?	NGDtZ5g1OMLizszN	MW[0POKhcA>?	Mo\qSG1UVw>?	NVTp[JNpcW6mdXPld{BidiCjY4TpeoF1cW:wIH;mJINie3Cjc3WgN{84	M4[4S\|I1QTl5NEWx
SW480	MW\BdI9xfG:\|aYOgRZN{[Xl?	M2TFfFQxyqEQvF2=	MXq0POKhcA>?	MV3EUXNQ	NF7VNnhqdmS3Y3XzJINp[W6pZYOgbY4hfGinIIDoc5NxcG:{eXzheIlwdiC\|dHH0eZMhd2ZiUGCyRUB1[XKpZYTz	MUeyOFk6PzR3MR?=
HT-29	NF71PYFCeG:ydH;zbZMhSXO\|YYm=	NXvhZpIyPDEEoN88US=>	NY\wT4FUPDkEoHi=	NUDCNHFiTE2VTx?=	NUnRXI5jcW6mdXPld{BkcGGwZ3XzJIlvKHSqZTDwbI9{eGixconsZZRqd25ic4TheJV{KG:oIGDQNmEhfGG{Z3X0dy=>	Mnz1NlQ6QTd2NUG=

... Click to View More Cell Line Experimental Data

Protocol

References

Solubility (25°C)

In vitro	DMSO	82 mg/mL (199.75 mM)
	Ethanol	37 mg/mL (90.13 mM)
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Forskolin SDF

Molecular Weight	410.5
Formula	C₂₂H₃₄O₇
CAS No.	66575-29-9
Storage	3 years -20°C powder
Storage	2 years -80°C in solvent
Synonyms	Coleonol

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2019-03-27)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01254006	Completed	Glaucoma	University of Roma La Sapienza\|vingolo enzo maria\|domanico daniela\|salvatore serena	null	Not Applicable
NCT01254006	Completed	Glaucoma	University of Roma La Sapienza\|vingolo enzo maria\|domanico daniela\|salvatore serena	null	Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Forskolin

Cited by 18 Publications

3 Customer Reviews

Purity & Quality Control

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Biological Activity

Protocol

References

Solubility (25°C)

Chemical Information Download Forskolin SDF

Bio Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Dilution Calculator

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

The Serial Dilution Calculator Equation

Serial Dilutions

Computed Result

Molecular Weight Calculator

Total Molecular Weight: g/mol

Instructions to calculate molar mass (molecular weight) of a chemical compound:

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2019-03-27)

Tech Support

cAMP Signaling Pathway Map

Choose Your Country or Region

By Signaling Pathways

By product type

Forskolin

Cited by 18 Publications

3 Customer Reviews

Purity & Quality Control

Choose Selective cAMP Inhibitors

Biological Activity

Protocol

References

Solubility (25°C)

Chemical Information Download Forskolin SDF

Bio Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Dilution Calculator

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

The Serial Dilution Calculator Equation

Serial Dilutions

Computed Result

Molecular Weight Calculator

Total Molecular Weight: g/mol

Instructions to calculate molar mass (molecular weight) of a chemical compound:

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2019-03-27)

Tech Support

cAMP Signaling Pathway Map

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)