Forskolin

For research use only.

Catalog No.S2449 Synonyms: Coleonol

50 publications

Forskolin Chemical Structure

Molecular Weight(MW): 410.5

Forskolin is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP in the study and research of cell physiology. Forskolin also activates PXR and FXR activity. Forskolin stimulates autophagy.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 140 In stock
USD 110 In stock
USD 270 In stock
USD 370 In stock
USD 570 In stock
USD 2990 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Forskolin has been cited by 50 publications

Purity & Quality Control

Choose Selective cAMP Inhibitors

Biological Activity

Description Forskolin is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP in the study and research of cell physiology. Forskolin also activates PXR and FXR activity. Forskolin stimulates autophagy.
Targets
Adenylyl cyclase (AC) [1]
(A wide variety of cell types)
In vitro

Forskolin increases cAMP levels in preparations of membranes, cells, or tissues. Forskolin not only activates AC but also interacts with certain other proteins, including glucose transporters and ion channels. Forskolin is able to promote activation of nine different transmembrane iso-forms of AC, albeit with somewhat less efficacy for AC9, which could be used to provides a means to identify and quantify high-affinity binding sites, i.e., G-proteins (Gs)–AC complexes. Activation of s by GPCRs contributes to Forskolin-stimulated cAMP generation in cells because of s-Forskolin potentiation of AC activity. [1] Forskolin stimulates adenylate cyclase activity without interacting with cell surface receptors. Forskolin's potentiation of cAMP in turn inhibits basophil and mast cell degranulation and histamine release, lowers blood pressure and intraocular pressure, inhibits platelet aggregation, promotes vasodilation, bronchodilation, and thyroid hormone secretion, and stimulates lipolysis in fat cells. Forskolin inhibits the binding of platelet-activating factor (PAF), independently of cAMP formation, which may be a result of Forskolin's direct effect on PAF or via interference with PAF binding to receptor sites. Forskolin also appears to have an effect on several membrane transport proteins, and inhibits glucose transport in erythrocytes, adipocytes, platelets, and other cells. Forskolin is used to treat with glaucoma. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 NV32N4NWTnWwY4Tpc44hSXO|YYm= NWD3[IJwOTBizszN MUC2JIg> NISyV2FqdmO{ZXHz[ZMheGixc4Doc5J6dGG2aX;uJI9nKG:4ZYLlfJBz\XO|ZXSgT2xJVDNiYYSgV|Q{Ow>? M3LSWlI3PDN3NEm4
Mo-DCs NVjPeWRETnWwY4Tpc44hSXO|YYm= MXK1NEDPxE1? MWSyOQKBkWh? NXrES|djeHKxbX;0[ZMhUUxvMkOgdJJw\HWldHnvckBqdiC2aHWgd5Vx\XKwYYThcpQhd2Zieontc5NidiC|dHnteYxifGWmIF3vMWREe8Li MXKyOlQyOjl2OB?=
RBMECs  MoHVSpVv[3Srb36gRZN{[Xl? M3rrbFXDqM7:TR?= MYKxxsBp M4LESoJtd2OtczD0bIUhWmGlMTDpcoFkfGm4YYTpc44hcW6mdXPl[EBjgSCHTVHQMWlK NFvacYwzPjN3OECzPS=>
INA-6 NXfG[4xuS2WubDDWbYFjcWyrdImgRZN{[Xl? NXLP[3BZOC1zMECg{txO M2DRPVcz6oDLaB?= NFjJOGdqdmS3Y3XzJINmdGxiZHXheIgh\G:|ZTDk[ZBmdmSnboTsfS=> MUeyOlMxPjZ{NB?=
OPM-2 M2X0VGNmdGxiVnnhZoltcXS7IFHzd4F6 MWSwMVExOCEQvF2= NHrKZZA4OuLCiXi= MnzFbY5lfWOnczDj[YxtKGSnYYToJIRwe2ViZHXw[Y5l\W62bIm= NEPCXlEzPjNyNk[yOC=>
U266 M1XTSGNmdGxiVnnhZoltcXS7IFHzd4F6 M37lWlAuOTByIN88US=> NIDzcpI4OuLCiXi= MoLWbY5lfWOnczDj[YxtKGSnYYToJIRwe2ViZHXw[Y5l\W62bIm= NV7HTlRIOjZ|ME[2NlQ>
H929 NHnMXnhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NGDj[YExNTFyMDFOwG0> MnnwO|LjiImq M3\BSIlv\HWlZYOgZ4VtdCCmZXH0bEBld3OnIHTldIVv\GWwdHz5 NWTVOoRzOjZ|ME[2NlQ>
RPMI 8226 MmLER4VtdCCYaXHibYxqfHliQYPzZZk> MUGwMVExOCEQvF2= M{\McFcz6oDLaB?= MlHtbY5lfWOnczDj[YxtKGSnYYToJIRwe2ViZHXw[Y5l\W62bIm= MoXSNlY{ODZ4MkS=
MDCK  MXXGeY5kfGmxbjDBd5NigQ>? NEH1Xm4yOCEEtV2= M1TzV|I1KGh? MUnEUXNQ M{DSN5VxemWpdXzheIV{KHSqZTDlfJBz\XO|aX;uJI9nKFSJRj5OtlEh[W6mIFPUS2bDqA>? NIr1SHEzPjJyMkO1Ni=>
MDCK  NEKwelBHfW6ldHnvckBCe3OjeR?= NVHUOZMzOTBiwsXN M4rrXFI1KGh? NWTmb|JbTE2VTx?= M3S5XIlvcGmkaYTzJJRp\SCrbnPy[YF{\WRiZYjwdoV{e2mxbjDv[kBHViClYYXz[YQh[nliVFfGMe6zOQ>? MnjONlYzODJ|NUK=
Spinal cords  NV[2[ZVPTnWwY4Tpc44hSXO|YYm= M4XK[VHDqM7:TR?= MUizNEBucW5? MnjGd5RqdXWuYYTld{BkSU2SIHzleoVtew>? M{\nbVI3OTJ4OUK2
BeWo  NFLNe3NHfW6ldHnvckBCe3OjeR?= NGjUTJczPeLCid88US=> NFfOPGozPC92OD:3NkBp NVjSfoVYdGWjZIOgeI8h[W5iaX7jdoVie2ViaX6geIhmKGW6cILld5Nqd25ib3[gc5Rp\XJiZoXzbY9vKG2jcnvldpM> NWrlPWpOOjZyNUO1OFk>
bovine oocytes MojMSpVv[3Srb36gRZN{[Xl? NWO4cnp6OTBywrFOwG0> MWOxNuKhcA>? M3fQV4lvcGmkaYTzJJRp\SCnZn\lZ5Qhd2ZiTmDQRUBidmRxb4KgUnBRSyC2bzDzeIlufWyjdHWgdoV{fW2ydHnvckBw\iCvZXnvd4l{ M2nUXVI3ODVzNkGx
Caco-2  M4rYS2Z2dmO2aX;uJGF{e2G7 MlnhNE4yNzFxMUCg{txO M{LGbFIxyqCvaX6= NUO5PW5zcW6mdXPld{BiKGSxc3Wt[IVx\W6mZX70JIlv[3KnYYPlJIlvKGmwdILhZ4VtdHWuYYKgZ2FOWCCuZY\lcJM> MVeyOlA1QTFyMh?=
Caco-2  NIC0U4lHfW6ldHnvckBCe3OjeR?= MWmwMlEwOS9zMDFOwG0> NEHJSVczPCCq MkXZbY5kemWjc3XzJG1TWDJicILveIVqdiCuZY\lcC=> MWGyOlA1QTFyMh?=
AML-12  MWfGeY5kfGmxbjDBd5NigQ>? MlP3NlAh|ryP M3T2cVMhcA>? MWL1dJJm\3WuYYTld{B1cGVicHjvd5Bpd3K7bHH0bY9vKGyndnXsd{BifCCWaIKtOFEyKGGwZDDT[ZIuPDl| MnvzNlYxPDh7OEW=
AML-12  M1;ndmZ2dmO2aX;uJGF{e2G7 M4DKXFIxKM7:TR?= M1[3eVEuQCCq Mon6bY5kemWjc3XzJIdtfWOxc3WgdJJw\HWldHnvci=> NECwT5AzPjB2OEm4OS=>
AML-12  Mk\FSpVv[3Srb36gRZN{[Xl? NEDnS5gzOCEQvF2= NVLIWXMzOyCq MkL5eZAuemWpdXzheIV{yqCSZ3OxZUzDqFCncHPrMEBidmUEoFe2dIPDqG2UTlGgcIV3\Wy| NFX4bWwzPjB2OEm4OS=>
AML-12  NInGZ|lHfW6ldHnvckBCe3OjeR?= MomzNlAh|ryP MXezJIg> MUTpcoR2[2W|IITo[UBl\XCqb4PwbI9zgWyjdHnvckBw\iCFUmTDNuKh M3zTWlI3ODR6OUi1
RBMECs NFT5cG5HfW6ldHnvckBCe3OjeR?= NYe4bINKPcLizszN M{LzeVEhcA>? NHnBVY1qdmirYnn0d{BGVUGSLVnJMYlv\HWlZXSgbY5i[3SrdnH0bY9vKG:oIGLhdFHDqA>? NG[xRmYzPjB2NE[2Ny=>
EndoC-βH1 MW\GeY5kfGmxbjDBd5NigQ>? MVW1xsDPxE1? MUKxJIg> Ml7QdI91\W62aXH0[ZMh\2y3Y3;z[U1qdmS3Y3XkJIlve3WuaX6gd4VkemW2aX;uJIlvKHSqZTDwdoV{\W6lZTDv[kBodHWlb4Pl M{ni[lI3ODJ6NU[y
EndoC-βH1 MofkSpVv[3Srb36gRZN{[Xl? M1i5d|XDqM7:TR?= M1W1ZVEhcA>? NWnBNXVmdGWjZIOgeI8h[SC|dILvcoch[0GPUDDpcoNz\WG|ZR?= NYLwbnkxOjZyMki1OlI>
SH-SY5Y M1XEcGZ2dmO2aX;uJGF{e2G7 M1zmW|MxKM7:TR?= NFXORZc{OCCvaX6= NUfVcZp7TE2VTx?= NYrSNXAxe2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgeIhmKGGldHn2ZZRqd25ib3[gVGtC NFr3bnMzPjB{NUGzOy=>
PC-3 MVXGeY5kfGmxbjDBd5NigQ>? MXW0NEDDvU1? MYqyJIg> MlvRSG1UVw>? NUf1cGdQdGWjZIOgeI8hWFB{QTDhZ5RqfmG2aX;u NE\jZZIzPjB{M{izOi=>
PC-3 NIXvVYNE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NH\i[Io1OCEEtV2= M3LkbFI1NzR6L{eyJIg> MVPEUXNQ NFPq[lll\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgeIlu\SCmZYDlcoRmdnSueR?= Mmr3NlYxOjN6M{[=
SCG MnLxSpVv[3Srb36gRZN{[Xl? M2myPFIxKM7:TdMg NGHCZm1FVVOR MkPqdoV3\XK|aXLsfUB{fXCycnXzd4V{KEmNVjD3bZRpKGFiSVO1NEBw\iB{ND60JO69VQ>? M3zwOlI2QTZ{MUOy
HEK-293 M4DHcmZ2dmO2aX;uJGF{e2G7 MorXN|Uh|ryPwrC= M4XBPGROW09? NILjV3JqdmS3Y3XzJIEh[2:wc4DpZ5VwfXNi4pEcbY5i[3SrdnH0bY9v6oDfIH;mJJRp\SCNdkKuNUBkfXK{ZX70 NE\xNFEzPTl4MkGzNi=>
SCG MkPZSpVv[3Srb36gRZN{[Xl? MVuxNFAh|ryPwrC= NF;kXVJFVVOR NXjmeXM5emWmdXPld{B1cGViZYjjbZRi[mmuaYT5JI9nKFOFRzDu[ZVzd26| NFPQ[lIzPTl4MkGzNi=>
PCCL3 M3;JTmZ2dmO2aX;uJGF{e2G7 NFHMdmoyOCEEtV2= NIfRelgzPCCq NI\1VXZmdmijbnPld{BFfU:6MjDwdo9ud3SncjD0doFve2O{aYD0bY9vKGGldHn2bZR6yqEkgJu= NVfZSVB{OjV7NkC5OVY>
3T3-L1 preadipocytes M4TONGZ2dmO2aX;uJGF{e2G7 NGPYU2EyOCEQvF5CpC=> MoPkNVIhcA>? NF;VNGpqdmS3Y3XzJGNTTUJicHjvd5Bpd3K7bHH0bY9vKGGwZDDDM2VDWM7{IHX4dJJme3Orb36= NVfGfnJYOjV7MkiwOVg>
H295R  M1XkcWZ2dmO2aX;uJGF{e2G7 M1jlVVExyqEQvF2= Mnz1OFjDqGh? MV;pcoNz\WG|ZYOgd5Rmem:rZDDt[ZRi[m:uaYTld{BqdiC2aHWgZY5lem:pZX6sJI1qdmW{YXzvMUBidmRiZ3z1Z49kd3K2aXPvbYQheGG2aIfhfZM> MV:yOVg3QTV3Nh?=
PBMC M{DncWZ2dmO2aX;uJGF{e2G7 MYS1NOKh|ryP NU\Kb4w5OjUEoHlCpC=> MojkbY5pcWKrdIOgeIhmKGmwY4LlZZNm\CC|ZXPy[ZRqd25ib3[gWG5HKGmwZIXj[YQh[nlidHjlJGRRTQ>? MVOyOVg3PjB5OR?=
GLUTag  NHfqNIVHfW6ldHnvckBCe3OjeR?= MVuxNQKBkcL3TR?= MWSwM|IwPCCq MXnzeIlufWyjdHXzJGdNWC1zIIPlZ5JmfGmxbjDjc5Rz\WG2ZXSge4l1cCCLQl3Y M{\sOVI2QDN{NkOx
GLUTag  MnW5SpVv[3Srb36gRZN{[Xl? MXKxNQKBkcL3TR?= M1iwR|QhcA>? M2PacYlv[3KnYYPld{B1cGVicFPSSWIhdGW4ZXzzJJdqfGhidHjlJGlDVVh? NWXQT2JVOjV6M{K2N|E>
BAECs NFPGdpBHfW6ldHnvckBCe3OjeR?= NGHwXYszPSEQvF2= Mlz4NlQhcA>? Mlvj[Y5p[W6lZYOgeIhmKGGldHn2ZZRqd25ib3[gVHBCWs7zIHL5JFUh|ryPIILld5ZmemG2cn;sMEBVPEiVLDDvdkA1NVCDUB?= MYOyOVc6QDh{Nh?=
PC12 MnjhSpVv[3Srb36gRZN{[Xl? MkTyNlXDqM7:TR?= M4GyeFQ5KGh? NVrVWJpJ[WO2aY\heIV{KGODTWC= M2Th[lI2PzZ7M{C1
GH3 M1\rfWZ2dmO2aX;uJGF{e2G7 MXqxxsDPxE1? M4fRbVYucA>? NWnrT3RY[XS2ZX71ZZRmeyC2aHWgZ49zemWuYYTpc44h[mW2d3XlckBRWkxiYX7kJGJu[WxzIHX4dJJme3Orb36= NFPWdFIzPTd{N{CxPC=>
GH3 M2[3PGZ2dmO2aX;uJGF{e2G7 MojtNeKh|ryP MkDnOk1p NH74cHJqdmS3Y3XzJHBTVCCjbnSgRo1idDFuIHL1eEBvd3RiQ3zvZ4stKG2UTlGg[ZhxemW|c3nvci=> MWmyOVczPzBzOB?=
BeWo NYGwR3I{TnWwY4Tpc44hSXO|YYm= M{LLOlEx6oDMzszNxsA> MmnuO|IhcA>? Mn3HSG1UVw>? M3\RSI1m\GmjdHXzJGJmX29iY3XscEBlcW[oZYLlcpRq[XSrb36= NYnZWohOOjV5MUO0NlU>
oocytes NEHxUYxHfW6ldHnvckBCe3OjeR?= M1jU[|Uh|ryP MVeyOEBp NILPS2pifHSnboXheIV{KHKqLXnud5VtcW5iYXP0bY9vKG:wIH;vZ5l1\SCJVlLEJJNq\26rZnnjZY51dHoEoB?= M3;CflI2PzB5OEW0
SC M3zM[mZ2dmO2aX;uJGF{e2G7 MVOwMlUh|ryP Mnv6NlQhcA>? M2\VOY1qdWmla4OgeIhmKGWoZnXjeEBw\iClQV3QJIFv[WyxZ4Ogc44hVzFiYX7kJG1DWCCneIDy[ZN{cW:w M1;hTlI2PzB3OEe0
SC MWDGeY5kfGmxbjDBd5NigQ>? MWGwMlUh|ryP M1fsXlczKGh? NVHyfIdHcW6lcnXhd4V{KGKxdHigT5JwgC1{MDDhcoQhVzFiZYjwdoV{e2mxbjDpckBigG:wLYLlcIF1\WRiU1PzJIJ2fCCxbnz5JGtzd3hvMkFCpC=> NF20T2kzPTdyNUi3OC=>
UACC-647  Ml\LSpVv[3Srb36gRZN{[Xl? M4jvdGROW09? NXHtfZVHdGWjZIOgeI8h[SC{aYPlJIlvKGODTWCgcIV3\Wy|IDjFR|UxyqB;wrCyNE4{QcLizszNLS=> NH3nfoEzPTdyM{CyOS=>
UACC-647  NEmzTYtHfW6ldHnvckBCe3OjeR?= NXH0OIl4OTEEoN88US=> NF7hTVkyPSCvaX6= Mmf0SG1UVw>? MXrpcohq[mm2czDFVmsheGixc4Doc5J6dGG2aX;u NXG4TphLOjV5MEOwNlU>
UACC-647  NWG0UGRvTnWwY4Tpc44hSXO|YYm= NXji[YM2OTEEoN88US=> MniyNVUhdWmw NFLlUGxFVVOR MnnKbY5kemWjc3XzJIVGTjJicHjvd5Bpd3K7bHH0bY9vKGyndnXsd:Kh MUmyOVcxOzB{NR?=
SH-SY5Y  NImwXYVHfW6ldHnvckBCe3OjeR?= M1XpZlExyqEQvF2= MnO3NeKhcMLi NUK3epZycW6lcnXhd4V{KEyXQzDhZ5Rqfmm2eR?= M2fTcVI2PTl5NEOz
SH-SY5Y  NWf5[4ZlTnWwY4Tpc44hSXO|YYm= MXexNOKh|ryP M2Hob|HDqGkEoB?= MlrtbY5kemWjc3XzJGFISzFibWLORUBt\X[nbB?= M4WxZ|I2PTl5NEOz
hADSCs M1\VUmZ2dmO2aX;uJGF{e2G7 M2\O[lXjiIoEtV2= MmDnN|AhdWmw M3ToXIlv[3KnYYPld{BkSU2SIHzleoVtew>? M13mSlI2PTlzOUC4
HEK293  NWH0bGdoTnWwY4Tpc44hSXO|YYm= NUjJc4RuPeLCidM1US=> NELLbYw{OCCvaX6= M4K2eYlv[3KnYYPld{BkSU2SIHzleoVtew>? NVLVfZZ7OjV3OUG5NFg>
3T3-L1 M3fjVmZ2dmO2aX;uJGF{e2G7 M4fOWVIvPS93IN88US=> MUWyOEBpyqB? MorEd4lodmmoaXPhcpRtgSCmZXPy[YF{\XNiQWTHUEBxem:2ZXnuJIV5eHKnc4Ppc44h[XRiYXzsJIRwe2W|IITld5Rm\A>? MWWyOVU6ODV7Nx?=
OCI-Ly1  MnnrSpVv[3Srb36gRZN{[Xl? Mo\zOFDDqM7:TR?= Ml\sNeKhcMLi M{PQWWROW09? MVTpcoR2[2W|IITo[UBqdmO{ZX3lcpQhd2ZiY1HNVEBkd26lZX70doF1cW:wcx?= NIn3fWEzPTV5NkKyNC=>
OCI-Ly18  NV\jSnhyTnWwY4Tpc44hSXO|YYm= M{LmflQxyqEQvF2= Mnn3NeKhcMLi MliySG1UVw>? NUDXPZhTcW6mdXPld{B1cGViaX7jdoVu\W62IH;mJINCVVBiY3;uZ4VvfHKjdHnvcpM> NGrsSlIzPTV5NkKyNC=>
BeWo M4rwUGZ2dmO2aX;uJGF{e2G7 NIjpOYUzOMLiwsXN MVG0POKhcA>? NYLUZoZKTE2VTx?= M1\SNYlv[3KnYYPld{B1cGViZHnm[oVz\W62aXH0bY9vKG:oIFLlW48h[2WubIO= NYP2O2RUOjV3Nk[3OFA>
BeWo M2jQbmZ2dmO2aX;uJGF{e2G7 MYmyNOKhyrWP NIG0NVY1QMLiaB?= NUXOXZlETE2VTx?= MXTpcoNz\WG|ZYOgeIhmKGGmaHXzbY9vKG:oIGTIVE0yKG2xbn;jfZRmew>? NVT0U4RbOjV3Nk[3OFA>
LNCaP  NV3veWoxTnWwY4Tpc44hSXO|YYm= MoTkNVDDqM7:TR?= MUKxNkBpyqB? MVXEUXNQ NGrNfHRqdmS3Y3XzJIEh\HKjbXH0bYMhcW6lcnXhd4Uhd2ZiQ2LFRlEh[WO2aY\peJk> M33QdVI2PTR6MEm5
ThGCs  MX3GeY5kfGmxbjDBd5NigQ>? M3LYXlEx6oDMzszN NVPtNXpEPOLCini= NInYeoFifWevZX70d{BJUUZzQTDs[ZZmdHNidHjheEB4\XKnIIP0bY12dGG2ZXSgZpkhS2:FbEK= NFnJ[mUzPTR|M{CyOy=>
ThGCs  NYT3TVFzTnWwY4Tpc44hSXO|YYm= MUSxNQKBks7:TR?= NG\Be3E16oDMaB?= MkP4bY5kemWjc3XzJGNwS2x{LXnu[JVk\WRiRVTONuKh\2WwZTDlfJBz\XO|aX;u NX7NeJo1OjV2M{OwNlc>
ThGCs  NGLxU4lHfW6ldHnvckBCe3OjeR?= NYf5RYxoOTEkgJtOwG0> NWDaZYhtOyCq Ml[4bY5pcWKrdIOgeIhmKGWoZnXjeEBw\iCKMl:yJI9vKEWGTkKgcXJPSQ>? M1HEfFI2PDN|MEK3
RBMECs NXPxWJZETnWwY4Tpc44hSXO|YYm= NFPpOIsxNjB3L{CuOU82KM7:TR?= MkjVNE4zPSCq M3\PR4lv[3KnYYPld{BkSU2SIHPvcoNmdnS{YYTpc44> MkDNNlU1OTZ4NUG=
RBMECs MWPGeY5kfGmxbjDBd5NigQ>? M4jYe|XDqM7:TR?= MVOxJIg> NHHw[HpjdG:la4OgeIhmKGGldHn2ZZRqd25ib3[gVohwSS:UT1PLJIlv\HWlZXSgZpkhTU2DUD3JTS=> MXeyOVQyPjZ3MR?=
RBMECs MXnGeY5kfGmxbjDBd5NigQ>? MYG1xsDPxE1? MV[xJIg> MXnwdoV3\W62czD0bIUhTU2DUD3JTU1qdmS3Y3XkJHRGTVJidnHseYUh\GWlcnXhd4U> M4jPdFI2PDF4NkWx
RBMECs MmLhSpVv[3Srb36gRZN{[Xl? NV7VOG1XPcLizszN NEXxZY8yKGh? MmjtdJJmfmWwdIOgeIhmKGmwY4LlZZNmKGmwIFjSVEBndHW6IHHjdo9{eyC2aHWgRnRDKGmwZIXj[YQh[nliIFXNRXAuUUl? MVuyOVQyPjZ3MR?=
RBMECs M4TrV2Z2dmO2aX;uJGF{e2G7 M3zGfFXDqM7:TR?= M3rzPFEhcA>? MWXpcohq[mm2czD0bIUh\GWlcnXhd4VlKG:oIHHtc5VvfCCxZjDaU{0yKGmwIF3Gd{BqdmS3Y3XkJIJ6KEWPQWCtTWk> NF34d4szPTRzNk[1NS=>
RBMECs NFnpVWRHfW6ldHnvckBCe3OjeR?= MkXrOeKh|ryP MYexJIg> NI[wNW5z\X[ncoPld{B1cGViY3jhcodmeyCrbjDaU{0yKGSrc4TybYJ2fGmxbjDz[YVvKHerdHigSW1CWC2LSTD0doVifG2nboS= M4[zTlI2PDF4NkWx
RBMECs NEPydYNHfW6ldHnvckBCe3OjeR?= Mmn5OeKh|ryP NFzlRo8yKGh? NFnjTXZjdG:la4OgeIhmKEWPQWCtTWkucW6mdXPl[EBkcGGwZ3WgbY4hVUyFIIDoc5NxcG:{eXzheIlwdg>? MXGyOVQyPjZ3MR?=
RBMECs MkDISpVv[3Srb36gRZN{[Xl? M3frVFXDqM7:TR?= NFe3[nQyKGh? NX6wXGJG[myxY3vzJJRp\SCjY4TpckBkgXSxc3vlcIV1d25icnXhdpJidmenbXXueEB{\WWwIIfpeIghTU2DUD3JTUB1emWjdH3lcpTDqA>? M2\2TFI2PDF4NkWx
Primary bovine chondrocytes NV;FU3NVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHf6bVU2|ryP NYLmbXVoPDhiaB?= M1K3O5JmfmW{c3XzJJRp\SCrbnjpZol1d3K7IHXm[oVkfCCxZjDj[Yxm[2:6aXKgc44heHKxbHnm[ZJifGmxbjDpckBoem:5dHigdIxifGViY3jvcoRzd2O7dHXz NX\6OoU5OjV2ME[wNVY>
EM1  NUfGbWZSTnWwY4Tpc44hSXO|YYm= M3ixSFE26oDMzszN NUfCSFhiPDhiaB?= M3;sepJm\HWlZYOgeIhmKGW6cILld5Nqd25ib3dCpGxKTsLib4NCpHBVT1N{wrDpcuKhS0GOUj2gc5LDqEWSQVOyMZNqdGWwY3XkJGVOOSClZXzsd:Kh M4LjT|I2Ozd6Nk[x
BeWo  MYPGeY5kfGmxbjDBd5NigQ>? NVfWfnI4OjEEoNM1US=> M1LxOFQ5yqCqwrC= NVjy[285TE2VT9Mg MljubY5kemWjc3XzJJRp\SCkZYThMYhETyC{ZXzlZZNm NXW4W2ttOjV|NkKyOlA>
BeWo  NUCwUI1wTnWwY4Tpc44hSXO|YYm= NVPRcldtOjEEoNM1US=> MU[0POKhcMLi NH3Y[JZFVVORwrC= Mmqw[I94dnKnZ4XsZZRmeyC2aHWgcIV3\Wxib3[gWG1GVUZzNh?= MmX0NlU{PjJ{NkC=
BeWo  NWHuSo16TnWwY4Tpc44hSXO|YYm= M3HrW|IxyqEEtV2= NY\QTWNrPDkEoHlCpC=> NUTqVYRkTE2VT9Mg NHGyNm1ld3ewcnXneYxifGW|IITo[UBt\X[nbDDv[kBIS01vMR?= M{Ty[lI2OzZ{Mk[w
granulosa cells NIfkcIFHfW6ldHnvckBCe3OjeR?= M1GxTFExKM7:TR?= MlW5NVIwOjRiaB?= NVLORVB5cW6lcnXhd4V{KHSqZTDs[ZZmdHNib3dCpHJIWzMEoH3SUmE> MlfrNlU{OzlzMEW=
granulosa cells NHLEOHpHfW6ldHnvckBCe3OjeR?= NHvXc5IyOCEQvF2= Mlm0NVIwOjRiaB?= NXKxeJBwcW6lcnXhd4V{KHSqZTDs[ZZmdHNib3[gdoVxd3K2ZYKgZYN1cX[rdImg[o9zKHSqZTDsc45o\XO2IH\yZYdu\W62IDlijLI5PTRxK{G4VmdUOi6OVVOp MXeyOVM{QTFyNR?=
granulosa cells MYrGeY5kfGmxbjDBd5NigQ>? MonINVAh|ryP NFK2NmIzPCCq NH[x[WhqdmO{ZXHz[ZMhfGinIHnueIVve2m2eTDv[kBFVkFxcILveIVqdiClb33wcIV5 NIfOb4szPTN|OUGwOS=>
granulosa cells NWHZTIxLTnWwY4Tpc44hSXO|YYm= NVLqTlF6OTBizszN MkXvNlQhcA>? M3;3U4lv[3KnYYPld{B1cGVibHX2[Yx{KG:oIGLHV|IheHKxbX;0[ZIh[WO2aY\peJk> NIXyZXozPTN|OUGwOS=>
SK-N-AS  NF;ae2xE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NFzleHoyOCEQvF5CpC=> MV6yOE81QCCq MV\lcohidmOnczD0bY1mNWSncHXu[IVvfGy7IHPlcIx2dGG{II\pZYJqdGm2edMg MV2yOVI3PjB4Mx?=
SK-N-AS  NHi0eFdHfW6ldHnvckBCe3OjeR?= NYntdpJWOTBizszNxsA> MU[yOEBp NWnt[GRpcW6lcnXhd4V{KHSqZTDjRW1RKGyndnXsd:Kh NWm3UG5sOjV{Nk[wOlM>
SK-N-AS  M2Hne2Z2dmO2aX;uJGF{e2G7 MXqxNEDPxE4EoB?= NFHDdmEzPCCq MVHpcoNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3[gZ5lkdGmwIFSx M1TpVlI2OjZ4ME[z
SK-N-AS  MWfGeY5kfGmxbjDBd5NigQ>? NGHqU|QyOCEQvF5CpC=> MVGzNEBucW5? MVnpcoR2[2W|IIDoc5NxcG:{eXzheIlwdiCxZjFOtk1k[XSnbnnuJEh{\XJ4N{WpMEBxNUeVS{ROtkApe2W{OTmgZY5lKGOxbnPvcYl1[W62IHjp[4hmeiCuZY\lcJMhd2ZiYXP0bZZmNCC3boDoc5NxcG:{eXzheIVlNCEQsj3jZZRmdmmw NFnZeZkzPTJ4NkC2Ny=>
SK-N-AS  NFfmVoFHfW6ldHnvckBCe3OjeR?= NHPw[5cyOCEQvF5CpC=> MlLWNVAwOzBxNkCgcYlv M2S4T4lv[3KnYYPld{Bt\X[nbIOgc4YheC4Qsj3jZZRmdmmwIDjz[ZI3PzVrIHHu[EBqdmS3Y3XzJIFk[3WvdXzheIlwdiCxZjDwMe6zNWOjdHXubY4hMHOnck[3OUkhcW5iKIDldokqdnWlbHXhdkBz\Werb37z NE\n[WUzPTJ4NkC2Ny=>
SK-N-SH M2jRfWNmdGxiVnnhZoltcXS7IFHzd4F6 Mn2wNVAh|ryPwrC= NWnPUWhoPDhiaB?= M3zKdIVvcGGwY3XzJHNMNU5vU1igcoV2em:kbHHzeI9u[SClZXzsJJZq[WKrbHn0fS=> NFu1U3YzPTJ4NkC2Ny=>
HEK‐CFTR M4\NbWZ2dmO2aX;uJGF{e2G7 MU[y5qCUPTEEoN88US=> NIDmNGsxNTF{IH3pci=> M4rKe2ROW00EoB?= NUTwfVN4cW6mdXPld{BiKGSxc3ZihLBl\XCnbnTlcpQhcW:maXTlJIVn\my3eNMg M3jpPVI2OjZ|MkC3
L6 M2PIWGZ2dmO2aX;uJGF{e2G7 MlzNOFAhyrWP MoHhNlQhcA>? NXjZOG0xcW6qaXLpeJMhTE2KMT3pcoR2[2WmIFHreEBi[3SrdnH0bY9v Mk[2NlUzPDd3NUC=
MIN6  M2r4dmZ2dmO2aX;uJGF{e2G7 MoX0NVAh|ryPwrC= NHXRRoQ{KGh? MkjmbY5kemWjc3XzJGQ{KG2UTlGg[ZhxemW|c3nvci=> MXmyOVI1OTF{NB?=
ventricular cardiomyocytes  MUTGeY5kfGmxbjDBd5NigQ>? M1vOblAvODFvMUCg{txO M3TSVolv[3KnYYPld{Ah[0GPUDDhZ4N2dXWuYYTpc44> M2HiW|I2OjB|MUGz
ventricular cardiomyocytes  NFL4[5BHfW6ldHnvckBCe3OjeR?= NIrOVWQxNjBzLUGwJO69VQ>? NW\VelZK\X[xa3XzJIFvKGmwb4Tyc5Bq[yC{ZYPwc45{\SBzMkFCtVE2LSCjYn;2[UBj[XOjbDD3bZRpKGGwIFXDOVDDqG:oIEKuNkDDvU1? NI\xUlQzPTJyM{GxNy=>
BeWo  MojISpVv[3Srb36gRZN{[Xl? MX[yNEDDvU1? M1\JUFQ5KGh? MU\EUXNQyqB? MYHpcoR2[2W|IHPlcIwh\nW|aX;u NVrv[HQ6OjVzOES0O|c>
THP-1  NHTqdYNHfW6ldHnvckBCe3OjeR?= NUDaWmVpOS9zMDFOwG0> NF;FboMzyqCq NXe2OFB{TE2VT9Mg NYnmfHo2e3WycILld5NmeyCPQ2CtNUBxem:mdXP0bY9vyqB? NYTyOJM2OjVzNUS4PFI>
Huh-7 NUPRblBFTnWwY4Tpc44hSXO|YYm= NFHHOpMxNTJyIN88US=> MYmyJIjDqA>? NH\1dXZz\XO3bITzJIlvKGFiZH;z[U1l\XCnbnTlcpQhcW6lcnXhd4UhcW5iYz3NfYMh\XiycnXzd4lwdiCjdDD0bIUheHKxdHXpckBidmRibWLORUBt\X[nbIO= NU\IOndOOjVzMEm4N|Q>
C6 NF:zcnBHfW6ldHnvckBCe3OjeR?= Mom3NVAh|ryPwrC= MUGyNEBucW5? NFXlWmpqdmO{ZXHz[ZMh[0GPUDDhZ4N2dXWuYYTpc44> MkXMNlUxPjl2MUe=
SW480 NU\NcFA3TnWwY4Tpc44hSXO|YYm= NXHPfmFjPDEEoN88US=> NGnOUlI1QMLiaB?= MoX4SG1UVw>? M1z0doFkfGm4YYTld{BRWDKD NFT4RpIzPDl7N{S1NS=>
HT-29  MYDGeY5kfGmxbjDBd5NigQ>? MVK0NOKh|ryP MYW0POKhcA>? M3vhTGROW09? M2[xdoFkfGm4YYTld{BRWDKD M4XHXlI1QTl5NEWx
SW480 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGXDOnI1OMLizszN NHmycYwxNTd{IHi= MUPEUXNQ NYPqTIlUcW6qaXLpeJMh[2WubDDndo94fGhidHnt[UBl\XCnbnTlcpRtgQ>? MVKyOFk6PzR3MR?=
HT-29  NFLYNHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXu0NOKh|ryP NX6zVFBJOC15MjDo Mni1SG1UVw>? Mn7wbY5pcWKrdIOgZ4VtdCCpcn;3eIghfGmvZTDk[ZBmdmSnboTsfS=> M{LMVVI1QTl5NEWx
SW480 NWTqZZV3TnWwY4Tpc44hSXO|YYm= M3PsblQxyqEQvF2= M{HM[Vch\A>? NH;FR|BFVVOR NGfQR|Nz\WS3Y3XzJINwdG:wb4PwbIVz\SCob4LtZZRqd25iY3HwZYJqdGm2edMg NHSxUYMzPDl7N{S1NS=>
HT-29  MVfGeY5kfGmxbjDBd5NigQ>? MXO0NOKh|ryP NVPqTWZ{PyCm M4DUNmROW09? NFXYd2Jz\WS3Y3XzJINwdG:wb4PwbIVz\SCob4LtZZRqd25iY3HwZYJqdGm2edMg NYLLXJpzOjR7OUe0OVE>
SW480 NIHKcppCeG:ydH;zbZMhSXO|YYm= Ml\HOFDDqM7:TR?= M4i5UFQ5yqCq MVXEUXNQ NV7nNpltcW6mdXPld{BidiCjY4TpeoF1cW:wIH;mJINie3Cjc3WgN{84 NUL3VlJuOjR7OUe0OVE>
HT-29  MUXBdI9xfG:|aYOgRZN{[Xl? M3jO[lQxyqEQvF2= MXu0POKhcA>? NGHtW|lFVVOR NGHnSppqdmS3Y3XzJIFvKGGldHn2ZZRqd25ib3[gZ4F{eGG|ZTCzM|c> MoC2NlQ6QTd2NUG=
SW480 MYLBdI9xfG:|aYOgRZN{[Xl? MWG0NOKh|ryP M4T4fFQ5yqCq MVzEUXNQ MofObY5lfWOnczDjbIFv\2W|IHnuJJRp\SCyaH;zdIhwenmuYYTpc44he3SjdIXzJI9nKFCSMlGgeIFz\2W2cx?= Mn\QNlQ6QTd2NUG=
HT-29  M3;ZcGFxd3C2b4Ppd{BCe3OjeR?= MX60NOKh|ryP NIOxUok1QMLiaB?= M3PqXGROW09? M{TWWYlv\HWlZYOgZ4hidmenczDpckB1cGVicHjvd5Bpd3K7bHH0bY9vKHO2YYT1d{Bw\iCSUELBJJRiemendIO= NXy3V5l4OjR7OUe0OVE>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
cleaved caspase-3 / caspase-3; 

PubMed: 30863177     


Toledo, NK-92, RL, and Farage cells were treated with 30 μM of forskolin for 48 hours, then the cells were harvested for Western blotting to assess the expression of total or cleaved caspase-3

cleaved caspase-9 / caspase-9 ; 

PubMed: 30863177     


Toledo, NK-92, RL, and Farage cells were treated with 30 μM of forskolin for 48 hours, then the cells were harvested for Western blotting to assess the expression of total or cleaved caspase-9.

β-catenin; 

PubMed: 30863177     


(A, B) The protein expression of cytoplasmic and nuclear β-catenin in Toledo and NK-92 cells in the presence of 0, 10, 20, or 40 μM forskolin was detected by Western blotting.

c-myc / Cyclin D1; 

PubMed: 30863177     


(C, D) Western blotting analysis of the total protein expression of c-myc and cyclin D1 influenced by 0, 10, 20, or 40 μM of forskolin in Toledo and NK-92 cells (n=3, *P<0.05, forskolin group vs control group).

pS6K1 / S6K1 / pCREB / CREB; 

PubMed: 31112131     


Forskolin inhibits mTORC1 in multiple cell lines. PC3, LNcap/AR, H1299, H1944, MDA-MB-231, MEF, HAP1, COS7, HeLa, MIA Paca-2, and PANC-1 were treated with or without 10 μM forskolin for 1 h and mTORC1 activity, CREB phosphorylation, and loading controls were analyzed.

p-JNK / JNK / P-p38 / p38; 

PubMed: 20070884     


L929 cells were pretreated with forskolin (10 μM) for various times as indicated, followed by stimulation with or without 10 ng/ml TNF-α for 15 min.

30863177 31112131 20070884
Immunofluorescence
5hmC; 

PubMed: 29239726     


Schwann cells were treated with forskolin (10 μM) for 3 hr followed by washout. 5hmC induction was detected at of 0, 3, and 24 hr time points following treatment. Cells continuously treated with forskolin (5, 10 μM) for 24 hr showed comparable 5hmC levels. Scale bar = 20 μm. 

Fe(II); 

PubMed: 29239726     


AMP (100 mM) treatment for 4 hr did not induce labile Fe(II) while AC activators (forskolin (100 mM), bicarbonate (50 mM)) and PDE inhibitors (caffeine (100 mM), IBMX (100 mM)) increased labile Fe(II) detected by Trx-Puro probes.

CYP17A1 / CYP21A2; 

PubMed: 25334044     


H295R cells were treated with 8-Br-cAMP or forskolin for 48 h under growth conditions, and fixed with 4% paraformaldehyde. (A) Immunostaining for CYP17A1. Red staining shows the anti-CYP17A1 antibody and blue staining shows DAPI (cell nuclei). (B) Immunostaining for CYP21A2. Red staining shows the anti-CYP21A2 antibody and blue staining shows DAPI (cell nuclei). Scale bars represent 100 µm.

29239726 25334044
Growth inhibition assay
Cell viability; 

PubMed: 30863177     


(C) MTT assay was conducted to test the cell growth after Toledo cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 1, 2, 3, 4, 5, or 6 days. (D) Toledo cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 48 hours, then MTT assay was used to evaluate the IC50 of forskolin. (F) MTT assay was conducted to test the cell growth after NK-92 cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 1, 2, 3, 4, 5, or 6 days. (G) NK-92 cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 48 hours, then MTT assay was used to evaluate the IC50of forskolin. 

30863177

Protocol

Solubility (25°C)

In vitro DMSO 82 mg/mL (199.75 mM)
Water Insoluble
Ethanol '37 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 410.5
Formula

C22H34O7

CAS No. 66575-29-9
Storage powder
in solvent
Synonyms Coleonol
Smiles CC(=O)OC1C(O)C2C(C)(C)CCC(O)C2(C)C3(O)C(=O)CC(C)(OC13C)C=C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

cAMP Signaling Pathway Map

Tags: buy Forskolin | Forskolin supplier | purchase Forskolin | Forskolin cost | Forskolin manufacturer | order Forskolin | Forskolin distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID