Forskolin

For research use only.

Catalog No.S2449 Synonyms: Coleonol

51 publications

Forskolin Chemical Structure

Molecular Weight(MW): 410.5

Forskolin is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP in the study and research of cell physiology. Forskolin also activates PXR and FXR activity. Forskolin stimulates autophagy.

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Selleck's Forskolin has been cited by 51 publications

Purity & Quality Control

Choose Selective cAMP Inhibitors

Biological Activity

Description Forskolin is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP in the study and research of cell physiology. Forskolin also activates PXR and FXR activity. Forskolin stimulates autophagy.
Targets
Adenylyl cyclase (AC) [1]
(A wide variety of cell types)
In vitro

Forskolin increases cAMP levels in preparations of membranes, cells, or tissues. Forskolin not only activates AC but also interacts with certain other proteins, including glucose transporters and ion channels. Forskolin is able to promote activation of nine different transmembrane iso-forms of AC, albeit with somewhat less efficacy for AC9, which could be used to provides a means to identify and quantify high-affinity binding sites, i.e., G-proteins (Gs)–AC complexes. Activation of s by GPCRs contributes to Forskolin-stimulated cAMP generation in cells because of s-Forskolin potentiation of AC activity. [1] Forskolin stimulates adenylate cyclase activity without interacting with cell surface receptors. Forskolin's potentiation of cAMP in turn inhibits basophil and mast cell degranulation and histamine release, lowers blood pressure and intraocular pressure, inhibits platelet aggregation, promotes vasodilation, bronchodilation, and thyroid hormone secretion, and stimulates lipolysis in fat cells. Forskolin inhibits the binding of platelet-activating factor (PAF), independently of cAMP formation, which may be a result of Forskolin's direct effect on PAF or via interference with PAF binding to receptor sites. Forskolin also appears to have an effect on several membrane transport proteins, and inhibits glucose transport in erythrocytes, adipocytes, platelets, and other cells. Forskolin is used to treat with glaucoma. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 MmPaSpVv[3Srb36gRZN{[Xl? NEDKeZAyOCEQvF2= M2rNS|YhcA>? MmTCbY5kemWjc3XzJJBpd3OyaH;yfYxifGmxbjDv[kBwfmW{ZYjwdoV{e2WmIFvMTGw{KGG2IGO0N|M> MlO1NlY1OzV2OUi=
Mo-DCs M1HyfWZ2dmO2aX;uJGF{e2G7 MljNOVAh|ryP MVmyOQKBkWh? M17vfJBzd22xdHXzJGlNNTJ|IIDyc4R2[3Srb36gbY4hfGinIIP1dIVzdmG2YX70JI9nKHq7bX;zZY4he3SrbYXsZZRm\CCPbz3ER5PDqA>? NUjXZndtOjZ2MUK5OFg>
RBMECs  MlX0SpVv[3Srb36gRZN{[Xl? MkTtOeKh|ryP M{nrfVHDqGh? MoPGZoxw[2u|IITo[UBT[WNzIHnuZYN1cX[jdHnvckBqdmS3Y3XkJIJ6KEWPQWCtTWk> NVyyN4FyOjZ|NUiwN|k>
INA-6 M33DSmNmdGxiVnnhZoltcXS7IFHzd4F6 M3fk[VAuOTByIN88US=> NWXGcGpNPzMkgJno MVXpcoR2[2W|IHPlcIwh\GWjdHig[I9{\SCmZYDlcoRmdnSueR?= MmDUNlY{ODZ4MkS=
OPM-2 MWTD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MVWwMVExOCEQvF2= M{LVcFcz6oDLaB?= M{OwRolv\HWlZYOgZ4VtdCCmZXH0bEBld3OnIHTldIVv\GWwdHz5 NIP5UJUzPjNyNk[yOC=>
U266 NX32OGY{S2WubDDWbYFjcWyrdImgRZN{[Xl? NWPRd2xoOC1zMECg{txO MmCwO|LjiImq M{HEOIlv\HWlZYOgZ4VtdCCmZXH0bEBld3OnIHTldIVv\GWwdHz5 MkXpNlY{ODZ4MkS=
H929 NX;BNo1nS2WubDDWbYFjcWyrdImgRZN{[Xl? M1PsS|AuOTByIN88US=> NV3YZYxYPzMkgJno NH7rO|NqdmS3Y3XzJINmdGxiZHXheIgh\G:|ZTDk[ZBmdmSnboTsfS=> NX3IO4xWOjZ|ME[2NlQ>
RPMI 8226 M3jodGNmdGxiVnnhZoltcXS7IFHzd4F6 MleyNE0yODBizszN M2HPNVcz6oDLaB?= NXrO[3hOcW6mdXPld{Bk\WyuIHTlZZRpKGSxc3Wg[IVx\W6mZX70cJk> M{S2SlI3OzB4NkK0
MDCK  MVnGeY5kfGmxbjDBd5NigQ>? NVrYWGE1OTBiwsXN NUfhNogzOjRiaB?= NEPUfHhFVVOR MkfweZBz\We3bHH0[ZMhfGinIHX4dJJme3Orb36gc4YhXEeILd8yNUBidmRiQ2THSuKh MoexNlYzODJ|NUK=
MDCK  NEXMUoxHfW6ldHnvckBCe3OjeR?= MXixNEDDvU1? Ml7xNlQhcA>? MWjEUXNQ NGm0W2FqdmirYnn0d{B1cGViaX7jdoVie2WmIHX4dJJme3Orb36gc4YhTk5iY3H1d4VlKGK7IGTHSk3PujF? M4rnOFI3OjB{M{Wy
Spinal cords  NFPpd5lHfW6ldHnvckBCe3OjeR?= NW[2PZRCOcLizszN MlnWN|AhdWmw NFLrWo5{fGmvdXzheIV{KGODTWCgcIV3\Wy| M1XFd|I3OTJ4OUK2
BeWo  MmPVSpVv[3Srb36gRZN{[Xl? NH;UNYozPeLCid88US=> MXeyOE81QC95MjDo NWS2NIxQdGWjZIOgeI8h[W5iaX7jdoVie2ViaX6geIhmKGW6cILld5Nqd25ib3[gc5Rp\XJiZoXzbY9vKG2jcnvldpM> M4jHZ|I3ODV|NUS5
bovine oocytes MYTGeY5kfGmxbjDBd5NigQ>? M4HGXFExOMLizszN NXP6Smh5OTMEoHi= MV\pcohq[mm2czD0bIUh\W[oZXP0JI9nKE6SUFGgZY5lN2:{IF7QVGMhfG9ic4TpcZVt[XSnIILld5VueHSrb36gc4YhdWWrb4Ppdy=> M{XuSFI3ODVzNkGx
Caco-2  M{T2RWZ2dmO2aX;uJGF{e2G7 NYT4V3JsOC5zL{GvNVAh|ryP NEnoOY8zOMLibXnu M3jDcIlv\HWlZYOgZUBld3OnLXTldIVv\GWwdDDpcoNz\WG|ZTDpckBqdnS{YXPlcIx2dGG{IHPBUXAhdGW4ZXzz M1PldlI3ODR7MUCy
Caco-2  MYHGeY5kfGmxbjDBd5NigQ>? MWiwMlEwOS9zMDFOwG0> Mn7RNlQhcA>? MVTpcoNz\WG|ZYOgUXJROiCycn;0[YlvKGyndnXs MnHGNlYxPDlzMEK=
AML-12  NHnrV4hHfW6ldHnvckBCe3OjeR?= MlXSNlAh|ryP MUOzJIg> MX71dJJm\3WuYYTld{B1cGVicHjvd5Bpd3K7bHH0bY9vKGyndnXsd{BifCCWaIKtOFEyKGGwZDDT[ZIuPDl| M3G1SFI3ODR6OUi1
AML-12  NV;4c|hZTnWwY4Tpc44hSXO|YYm= NXXxVnkyOjBizszN NYmzS4V1OS16IHi= M{[1[Ilv[3KnYYPld{BodHWlb4PlJJBzd2S3Y4Tpc44> Moq3NlYxPDh7OEW=
AML-12  M1K1R2Z2dmO2aX;uJGF{e2G7 NWrjXZpVOjBizszN NHywbIw{KGh? MmnleZAuemWpdXzheIV{yqCSZ3OxZUzDqFCncHPrMEBidmUEoFe2dIPDqG2UTlGgcIV3\Wy| M1jY[FI3ODR6OUi1
AML-12  M2rROWZ2dmO2aX;uJGF{e2G7 MVOyNEDPxE1? NUHNbZQ6OyCq NW\oW4xEcW6mdXPld{B1cGViZHXwbI9{eGixconsZZRqd25ib3[gR3JVSzMEoB?= MYiyOlA1QDl6NR?=
RBMECs NHHVT3RHfW6ldHnvckBCe3OjeR?= MlnBOeKh|ryP M3PSN|EhcA>? NE\xbXdqdmirYnn0d{BGVUGSLVnJMYlv\HWlZXSgbY5i[3SrdnH0bY9vKG:oIGLhdFHDqA>? NVjE[np1OjZyNES2OlM>
EndoC-βH1 MULGeY5kfGmxbjDBd5NigQ>? MXG1xsDPxE1? M{DuW|EhcA>? M{XqOJBwfGWwdHnheIV{KGeudXPvd4UucW6mdXPl[EBqdnO3bHnuJJNm[3KndHnvckBqdiC2aHWgdJJme2WwY3Wgc4Yh\2y3Y3;z[S=> MYCyOlAzQDV4Mh?=
EndoC-βH1 M3vJPGZ2dmO2aX;uJGF{e2G7 NXuxSFhOPcLizszN M1\vc|EhcA>? NHL0c2tt\WGmczD0c{BiKHO2cn;u[{BkSU2SIHnuZ5Jm[XOn MXyyOlAzQDV4Mh?=
SH-SY5Y NEP1b5FHfW6ldHnvckBCe3OjeR?= MY[zNEDPxE1? NVPpe3FnOzBibXnu Ml\tSG1UVw>? NH\2UmR{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczD0bIUh[WO2aY\heIlwdiCxZjDQT2E> NITTPY4zPjB{NUGzOy=>
PC-3 MonhSpVv[3Srb36gRZN{[Xl? NIXBdZg1OCEEtV2= MXmyJIg> Ml\mSG1UVw>? NF3Nd4Zt\WGmczD0c{BRWDKDIHHjeIl3[XSrb36= MlTlNlYxOjN6M{[=
PC-3 NXz3PZlGS2WubDDWbYFjcWyrdImgRZN{[Xl? M{jzdVQxKML3TR?= M{LK[|I1NzR6L{eyJIg> MWDEUXNQ M4HPfYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTD0bY1mKGSncHXu[IVvfGy7 NY\RXHVTOjZyMkO4N|Y>
SCG M{P4NGZ2dmO2aX;uJGF{e2G7 MmjrNlAh|ryPwrC= MYLEUXNQ M3vSVJJmfmW{c3nicJkhe3WycILld5NmeyCLS2[ge4l1cCCjIFnDOVAhd2ZiMkSuOEDPxE1? NUfGTXJZOjV7NkKxN|I>
HEK-293 M1\SbWZ2dmO2aX;uJGF{e2G7 MlzuN|Uh|ryPwrC= M4TlbWROW09? NVXubIJLcW6mdXPld{BiKGOxboPwbYN2d3W|IPMAoIlv[WO2aY\heIlwduLCnTDv[kB1cGViS4[yMlEh[3W{cnXueC=> MWqyOVk3OjF|Mh?=
SCG MlrCSpVv[3Srb36gRZN{[Xl? M2rzcFExOCEQvF5CpC=> NVrib3FWTE2VTx?= NHfIbYRz\WS3Y3XzJJRp\SCneHPpeIFjcWyrdImgc4YhW0OJIH7leZJwdnN? NXvyV5lnOjV7NkKxN|I>
PCCL3 NVK0bGppTnWwY4Tpc44hSXO|YYm= NFG1TWwyOCEEtV2= MkHmNlQhcA>? NWTKS2Zs\W6qYX7j[ZMhTHWReEKgdJJwdW:2ZYKgeJJidnOlcnnweIlwdiCjY4Tpeol1gcLi4pEL NFHLeWwzPTl4MEm1Oi=>
3T3-L1 preadipocytes MX;GeY5kfGmxbjDBd5NigQ>? Ml\BNVAh|ryPwrC= NGfaRZUyOiCq MYDpcoR2[2W|IFPSSWIheGixc4Doc5J6dGG2aX;uJIFv\CCFL1XCVO6zKGW6cILld5Nqd25? MVuyOVkzQDB3OB?=
H295R  MVvGeY5kfGmxbjDBd5NigQ>? NVPlN|VIOTEEoN88US=> NV;wb3hoPDkEoHi= MWfpcoNz\WG|ZYOgd5Rmem:rZDDt[ZRi[m:uaYTld{BqdiC2aHWgZY5lem:pZX6sJI1qdmW{YXzvMUBidmRiZ3z1Z49kd3K2aXPvbYQheGG2aIfhfZM> NIixW|czPTh4OUW1Oi=>
PBMC Mm\JSpVv[3Srb36gRZN{[Xl? NVeySYdOPTEEoN88US=> MnvINlTDqGkEoB?= MXLpcohq[mm2czD0bIUhcW6lcnXhd4VlKHOnY4LleIlwdiCxZjDUUmYhcW6mdXPl[EBjgSC2aHWgSHBG M1jwOFI2QDZ4MEe5
GLUTag  NWfrdYRZTnWwY4Tpc44hSXO|YYm= MYqxNQKBkcL3TR?= M2fRO|AwOi92IHi= M2PPU5N1cW23bHH0[ZMhT0ySLUGgd4VkemW2aX;uJINwfHKnYYTl[EB4cXSqIFnCUXg> MV2yOVg{OjZ|MR?=
GLUTag  M4PxRmZ2dmO2aX;uJGF{e2G7 M{XSSFEx6oDLwsXN MoH3OEBp MX;pcoNz\WG|ZYOgeIhmKHCFUlXCJIxmfmWuczD3bZRpKHSqZTDJRm1Z NGLDSnYzPTh|Mk[zNS=>
BAECs MVnGeY5kfGmxbjDBd5NigQ>? MUiyOUDPxE1? M4j1c|I1KGh? MXXlcohidmOnczD0bIUh[WO2aY\heIlwdiCxZjDQVGFT|rFiYomgOUDPxE1icnXzeoVz[XS{b3ysJHQ1UFNuIH;yJFQuWEGS MVuyOVc6QDh{Nh?=
PC12 MX;GeY5kfGmxbjDBd5NigQ>? NGLtVmYzPcLizszN Mk\4OFghcA>? NWn5UYh7[WO2aY\heIV{KGODTWC= MYiyOVc3QTNyNR?=
GH3 M3jVdGZ2dmO2aX;uJGF{e2G7 M3LyV|HDqM7:TR?= Mn63Ok1p NXvGPYtp[XS2ZX71ZZRmeyC2aHWgZ49zemWuYYTpc44h[mW2d3XlckBRWkxiYX7kJGJu[WxzIHX4dJJme3Orb36= M13zblI2PzJ5MEG4
GH3 NV6yS2U4TnWwY4Tpc44hSXO|YYm= MVOxxsDPxE1? MnuzOk1p NYTrXnFScW6mdXPld{BRWkxiYX7kJGJu[WxzLDDieZQhdm:2IFPsc4NsNCCvUl7BJIV5eHKnc4Ppc44> M{nCWlI2PzJ5MEG4
BeWo M3z5PGZ2dmO2aX;uJGF{e2G7 NWq4SndFOTEkgJtOwG3DqA>? NFfBbIs4OiCq NFW2W49FVVOR NITqSFRu\WSrYYTld{BD\VexIHPlcIwh\GmoZnXy[Y51cWG2aX;u NHnJb2MzPTdzM{SyOS=>
oocytes Mme1SpVv[3Srb36gRZN{[Xl? NVXQXYp7PSEQvF2= MWGyOEBp M3jKb4F1fGWwdXH0[ZMhemhvaX7zeYxqdiCjY4Tpc44hd25ib3;jfZRmKEeYQlSgd4lodmmoaXPhcpRtgcLi NG\XWmgzPTdyN{i1OC=>
SC NITUVI1HfW6ldHnvckBCe3OjeR?= Mnz5NE42KM7:TR?= MWCyOEBp NUHiTGpWdWmvaXPrd{B1cGViZX\m[YN1KG:oIHPBUXAh[W6jbH;nd{BwdiCRMTDhcoQhVUKSIHX4dJJme3Orb36= NGnOflEzPTdyNUi3OC=>
SC NUjsfnZYTnWwY4Tpc44hSXO|YYm= MkPuNE42KM7:TR?= NXPOZ5VDPzJiaB?= M330dYlv[3KnYYPld{Bjd3SqIFvyc5guOjBiYX7kJG8yKGW6cILld5Nqd25iaX6gZZhwdi2{ZXzheIVlKFOFczDieZQhd26ueTDLdo95NTJywrC= NGjOeokzPTdyNUi3OC=>
UACC-647  M4LXN2Z2dmO2aX;uJGF{e2G7 MmHxSG1UVw>? MlH3cIVi\HNidH:gZUBzcXOnIHnuJINCVVBibHX2[Yx{KCiHQ{WwxsA:yqB{MD6zPeKh|ryPKR?= MYmyOVcxOzB{NR?=
UACC-647  Mn;wSpVv[3Srb36gRZN{[Xl? MlyzNVDDqM7:TR?= M4HVRlE2KG2rbh?= Mn3kSG1UVw>? NHnrVWFqdmirYnn0d{BGWkticHjvd5Bpd3K7bHH0bY9v NWHqdGFVOjV5MEOwNlU>
UACC-647  Mk[zSpVv[3Srb36gRZN{[Xl? M2K4TFExyqEQvF2= NEW3VY4yPSCvaX6= MnjDSG1UVw>? MnPkbY5kemWjc3XzJIVGTjJicHjvd5Bpd3K7bHH0bY9vKGyndnXsd:Kh NU[5VndTOjV5MEOwNlU>
SH-SY5Y  MmTsSpVv[3Srb36gRZN{[Xl? NV3mbpBDOTEEoN88US=> MoPyNeKhcMLi MU\pcoNz\WG|ZYOgUHVEKGGldHn2bZR6 MnjZNlU2QTd2M{O=
SH-SY5Y  M4PRSGZ2dmO2aX;uJGF{e2G7 NGexZmgyOMLizszN M2\0[VHDqGkEoB?= MUDpcoNz\WG|ZYOgRWdEOSCvUl7BJIxmfmWu NU\VVoh5OjV3OUe0N|M>
hADSCs M{i4e2Z2dmO2aX;uJGF{e2G7 NVS5WllvPeLCidM1US=> NGW3Nm8{OCCvaX6= MnPsbY5kemWjc3XzJINCVVBibHX2[Yx{ MVOyOVU6OTlyOB?=
HEK293  NFzPVI9HfW6ldHnvckBCe3OjeR?= NGnzb5I26oDLwsXN M1vPNVMxKG2rbh?= MVfpcoNz\WG|ZYOgZ2FOWCCuZY\lcJM> NHnSUZYzPTV7MUmwPC=>
3T3-L1 NWjDUWlzTnWwY4Tpc44hSXO|YYm= NEDVUIgzNjVxNTFOwG0> MXSyOEBpyqB? Mmfxd4lodmmoaXPhcpRtgSCmZXPy[YF{\XNiQWTHUEBxem:2ZXnuJIV5eHKnc4Ppc44h[XRiYXzsJIRwe2W|IITld5Rm\A>? Mnf5NlU2QTB3OUe=
OCI-Ly1  NHzlNGdHfW6ldHnvckBCe3OjeR?= NF;4[4U1OMLizszN MVGxxsBpyqB? NFfLPHlFVVOR NUjQXpZzcW6mdXPld{B1cGViaX7jdoVu\W62IH;mJINCVVBiY3;uZ4VvfHKjdHnvcpM> NVLXTZZHOjV3N{[yNlA>
OCI-Ly18  M2Tmc2Z2dmO2aX;uJGF{e2G7 NWHIfVFnPDEEoN88US=> NWrPfmRXOcLiaNMg M4nEd2ROW09? NEfKR5hqdmS3Y3XzJJRp\SCrbnPy[Y1mdnRib3[gZ2FOWCClb37j[Y51emG2aX;udy=> MoT4NlU2PzZ{MkC=
BeWo Mo\kSpVv[3Srb36gRZN{[Xl? MlLPNlDDqML3TR?= NYK3fVFUPDkEoHi= NFfsO5NFVVOR NEfYVmpqdmO{ZXHz[ZMhfGinIHTp[oZmemWwdHnheIlwdiCxZjDC[XdwKGOnbHzz M{\X[FI2PTZ4N{Sw
BeWo NFjEeYdHfW6ldHnvckBCe3OjeR?= M{G4NlIxyqEEtV2= M1m2RVQ5yqCq M4jafWROW09? Mn\XbY5kemWjc3XzJJRp\SCjZHjld4lwdiCxZjDUTHAuOSCvb37vZ5l1\XN? MVWyOVU3Pjd2MB?=
LNCaP  NU\5bJR[TnWwY4Tpc44hSXO|YYm= NV7Qd3ZzOTEEoN88US=> M4qz[|EzKGkEoB?= NHjRXGpFVVOR MlHXbY5lfWOnczDhJIRz[W2jdHnjJIlv[3KnYYPlJI9nKEOURVKxJIFkfGm4aYT5 Mm\1NlU2PDhyOUm=
ThGCs  M1LoWmZ2dmO2aX;uJGF{e2G7 NEPqXGQyOOLCit88US=> M2DiUFTjiIqq MVTheYdu\W62czDITWYySSCuZY\lcJMhfGijdDD3[ZJmKHO2aX31cIF1\WRiYomgR49EdDJ? NHLrSpMzPTR|M{CyOy=>
ThGCs  MYLGeY5kfGmxbjDBd5NigQ>? NW\WV2NuOTEkgJtOwG0> MUi05qCLcA>? MV3pcoNz\WG|ZYOgR49EdDJvaX7keYNm\CCHRF6yxsBo\W6nIHX4dJJme3Orb36= MVOyOVQ{OzB{Nx?=
ThGCs  NXzWbnV3TnWwY4Tpc44hSXO|YYm= NYPwZmRWOTEkgJtOwG0> MonMN{Bp NI\5ZWxqdmirYnn0d{B1cGViZX\m[YN1KG:oIFiyU|Ihd25iRVTONkBuWk6D MmXoNlU1OzNyMke=
RBMECs NIPzdIpHfW6ldHnvckBCe3OjeR?= NEK0bGkxNjB3L{CuOU82KM7:TR?= M2fZeVAvOjViaB?= NInzc2VqdmO{ZXHz[ZMh[0GPUDDjc45k\W62cnH0bY9v MWeyOVQyPjZ3MR?=
RBMECs NVLOZWx3TnWwY4Tpc44hSXO|YYm= M4m4e|XDqM7:TR?= M4\4dFEhcA>? NWjmc2ZI[myxY3vzJJRp\SCjY4TpeoF1cW:wIH;mJHJpd0FxUl;DT{BqdmS3Y3XkJIJ6KEWPQWCtTWk> M2ixSVI2PDF4NkWx
RBMECs MUfGeY5kfGmxbjDBd5NigQ>? NEHkcpo2yqEQvF2= MlvXNUBp MkXOdJJmfmWwdIOgeIhmKEWPQWCtTWkucW6mdXPl[EBVTUWUII\hcJVmKGSnY4LlZZNm NHzWOFYzPTRzNk[1NS=>
RBMECs Mn:3SpVv[3Srb36gRZN{[Xl? NYS3b2FsPcLizszN MUKxJIg> MYfwdoV3\W62czD0bIUhcW6lcnXhd4UhcW5iSGLQJIZtfXhiYXPyc5N{KHSqZTDCWGIhcW6mdXPl[EBjgSBiRV3BVE1KUQ>? NVzDO4hIOjV2MU[2OVE>
RBMECs NY\tfZlDTnWwY4Tpc44hSXO|YYm= M4foT|XDqM7:TR?= NVXFPHRkOSCq MoKxbY5pcWKrdIOgeIhmKGSnY4LlZZNm\CCxZjDhcY92dnRib3[gXm8uOSCrbjDNSpMhcW6mdXPl[EBjgSCHTVHQMWlK NVz6XW1sOjV2MU[2OVE>
RBMECs M{nmdWZ2dmO2aX;uJGF{e2G7 MUi1xsDPxE1? M120cFEhcA>? NUficYJIemW4ZYLz[ZMhfGinIHPoZY5o\XNiaX6gXm8uOSCmaYP0doljfXSrb36gd4VmdiC5aYToJGVOSVBvSVmgeJJm[XSvZX70 MXqyOVQyPjZ3MR?=
RBMECs M4HrXGZ2dmO2aX;uJGF{e2G7 NVThR4REPcLizszN MlzmNUBp NVfZSGZ6[myxY3vzJJRp\SCHTVHQMWlKNWmwZIXj[YQh[2ijbnflJIlvKE2OQzDwbI9{eGixconsZZRqd25? NVzhd5BVOjV2MU[2OVE>
RBMECs Mo\aSpVv[3Srb36gRZN{[Xl? NIjpT|g2yqEQvF2= M3PBRVEhcA>? MXzicI9kc3NidHjlJIFkfGmwIHP5eI9{c2WuZYTvckBz\WG{cnHu[4Vu\W62IIPl[Y4hf2m2aDDFUWFRNUmLIITy[YF1dWWwdNMg MnXwNlU1OTZ4NUG=
Primary bovine chondrocytes MkXaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXye2c2|ryP M4\FN|Q5KGh? MXfy[ZZmenOnczD0bIUhcW6qaXLpeI9zgSCnZn\lZ5Qhd2ZiY3Xs[YNwgGmkIH;uJJBzd2yrZnXyZZRqd25iaX6g[5Jwf3SqIIDsZZRmKGOqb37kdo9kgXSncx?= MU[yOVQxPjBzNh?=
EM1  NEnqeo9HfW6ldHnvckBCe3OjeR?= NH\jTGsyPeLCit88US=> MmTLOFghcA>? MnTsdoVlfWOnczD0bIUh\XiycnXzd4lwdiCxZtMgUGlHyqCxctMgVHRIWzMEoHnuxsBESUyULTDvduKhTVCDQ{Ktd4lt\W6lZXSgSW0yKGOnbHzzxsA> MlzJNlU{Pzh4NkG=
BeWo  MlrVSpVv[3Srb36gRZN{[Xl? MmL2NlDDqML3TR?= M1fUeFQ5yqCqwrC= NVTKdIVqTE2VT9Mg MmrQbY5kemWjc3XzJJRp\SCkZYThMYhETyC{ZXzlZZNm MmC2NlU{PjJ{NkC=
BeWo  MoH0SpVv[3Srb36gRZN{[Xl? NG\neFkzOMLiwsXN NETFNXI1QMLiaNMg M{O2WmROW00EoB?= NV3uTVdV\G:5boLl[5Vt[XSnczD0bIUhdGW4ZXygc4YhXE2HTV[xOi=> MV[yOVM3OjJ4MB?=
BeWo  M4T1WGZ2dmO2aX;uJGF{e2G7 NYexdFZrOjEEoNM1US=> NIrSOVI1QMLiaNMg MmTRSG1UV8Li M{K4OYRwf26{ZXf1cIF1\XNidHjlJIxmfmWuIH;mJGdEVS1z NEPmeHczPTN4MkK2NC=>
granulosa cells NHTrUWZHfW6ldHnvckBCe3OjeR?= M4i5PFExKM7:TR?= M2fLPFEzNzJ2IHi= Mn3jbY5kemWjc3XzJJRp\SCuZY\lcJMhd2cEoGLHV|LDqG2UTlG= MmnrNlU{OzlzMEW=
granulosa cells NVHzWFN1TnWwY4Tpc44hSXO|YYm= NI[5e5EyOCEQvF2= NYXaepBPOTJxMkSgbC=> NYjHXpFLcW6lcnXhd4V{KHSqZTDs[ZZmdHNib3[gdoVxd3K2ZYKgZYN1cX[rdImg[o9zKHSqZTDsc45o\XO2IH\yZYdu\W62IDlijLI5PTRxK{G4VmdUOi6OVVOp MV[yOVM{QTFyNR?=
granulosa cells NHXs[npHfW6ldHnvckBCe3OjeR?= NInJWFUyOCEQvF2= MmS1NlQhcA>? MYfpcoNz\WG|ZYOgeIhmKGmwdHXud4l1gSCxZjDEUmEweHKxdHXpckBkd22ybHX4 M2PIeVI2OzN7MUC1
granulosa cells MXXGeY5kfGmxbjDBd5NigQ>? NYHFPW5POTBizszN Mk\nNlQhcA>? MWrpcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iCUR2OyJJBzd22xdHXyJIFkfGm4aYT5 NHLwWXAzPTN|OUGwOS=>
SK-N-AS  NHHKVVlE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1K0[FExKM7:TdMg NXTrTXI5OjRxNEigbC=> NX\0[5NZ\W6qYX7j[ZMhfGmvZT3k[ZBmdmSnboTsfUBk\WyudXzhdkB3cWGkaXzpeJnDqA>? NIrYWWszPTJ4NkC2Ny=>
SK-N-AS  NH3jOG5HfW6ldHnvckBCe3OjeR?= NXSwcmU2OTBizszNxsA> NXz1V4FxOjRiaB?= MmTIbY5kemWjc3XzJJRp\SClQV3QJIxmfmWuc9Mg M{LtT|I2OjZ4ME[z
SK-N-AS  Mk\aSpVv[3Srb36gRZN{[Xl? NI\DNnQyOCEQvF5CpC=> MmnZNlQhcA>? MYrpcoNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3[gZ5lkdGmwIFSx NV:zSYh7OjV{Nk[wOlM>
SK-N-AS  MWTGeY5kfGmxbjDBd5NigQ>? M1jWZVExKM7:TdMg NXHYepgxOzBibXnu M3\Dcolv\HWlZYOgdIhwe3Cqb4L5cIF1cW:wIH;mJO6zNWOjdHXubY4hMHOnck[3OUktKHBvR2PLN:6zKCi|ZYK5LUBidmRiY3;uZ49ucXSjboSgbIlocGW{IHzleoVteyCxZjDhZ5RqfmVuIIXudIhwe3Cqb4L5cIF1\WRuIN8yMYNifGWwaX6= MkW2NlUzPjZyNkO=
SK-N-AS  MnfhSpVv[3Srb36gRZN{[Xl? NXXHOVBJOTBizszNxsA> MVSxNE8{OC94MDDtbY4> MYTpcoNz\WG|ZYOgcIV3\Wy|IH;mJJAu|rJvY3H0[Y5qdiBqc3XyOlc2MSCjbnSgbY5lfWOnczDhZ4N2dXWuYYTpc44hd2ZicD5Otk1k[XSnbnnuJEh{\XJ4N{WpJIlvKCiyZYLpLY52[2ynYYKgdoVocW:wcx?= M3rMfFI2OjZ4ME[z
SK-N-SH NF;1fG1E\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1L4VFExKM7:TdMg M3\lZlQ5KGh? M2LqR4VvcGGwY3XzJHNMNU5vU1igcoV2em:kbHHzeI9u[SClZXzsJJZq[WKrbHn0fS=> MXGyOVI3PjB4Mx?=
HEK‐CFTR MXnGeY5kfGmxbjDBd5NigQ>? NHTLbZYz6oDVNUFCpO69VQ>? Mm\wNE0yOiCvaX6= NFnYUlJFVVORwrC= NWqybZU3cW6mdXPld{BiKGSxc3ZihLBl\XCnbnTlcpQhcW:maXTlJIVn\my3eNMg Mm[5NlUzPjN{MEe=
L6 NWT6PXI{TnWwY4Tpc44hSXO|YYm= M2Di[lQxKML3TR?= NVvF[IJ5OjRiaB?= MmjzbY5pcWKrdIOgSG1JOS2rbnT1Z4VlKEGtdDDhZ5RqfmG2aX;u NV3JUmRWOjV{NEe1OVA>
MIN6  NFGxT|RHfW6ldHnvckBCe3OjeR?= Mmf3NVAh|ryPwrC= MnL5N{Bp M2PDXYlv[3KnYYPld{BFOyCvUl7BJIV5eHKnc4Ppc44> M3TSdlI2OjRzMUK0
ventricular cardiomyocytes  MXrGeY5kfGmxbjDBd5NigQ>? M2DtTFAvODFvMUCg{txO NFixV|hqdmO{ZXHz[ZMhKGODTWCgZYNkfW23bHH0bY9v M2XEWlI2OjB|MUGz
ventricular cardiomyocytes  NHi1O2NHfW6ldHnvckBCe3OjeR?= NHe0UlIxNjBzLUGwJO69VQ>? NEPFTJZmfm:tZYOgZY4hcW6xdILvdIlkKHKnc4DvcpNmKDF{MNMxNVUmKGGkb4\lJIJie2GuIIfpeIgh[W5iRVO1NOKhd2ZiMj6yJOK2VQ>? Ml3CNlUzODNzMUO=
BeWo  MX3GeY5kfGmxbjDBd5NigQ>? NI\Zd4QzOCEEtV2= M2PHNFQ5KGh? M1WwZ2ROW00EoB?= Ml[wbY5lfWOnczDj[YxtKG[3c3nvci=> NUPRdIZxOjVzOES0O|c>
THP-1  MlnXSpVv[3Srb36gRZN{[Xl? M37OeFEwOTBizszN MWGyxsBp M2faWmROW00EoB?= M3K1WJN2eHC{ZYPz[ZMhVUOSLUGgdJJw\HWldHnvcuKh MXyyOVE2PDh6Mh?=
Huh-7 NVnvTnJvTnWwY4Tpc44hSXO|YYm= NF3VPXkxNTJyIN88US=> Mne2NkBpyqB? MlHWdoV{fWy2czDpckBiKGSxc3Wt[IVx\W6mZX70JIlv[3KnYYPlJIlvKGNvTYnjJIV5eHKnc4Ppc44h[XRidHjlJJBzd3SnaX6gZY5lKG2UTlGgcIV3\Wy| MYKyOVExQTh|NB?=
C6 MXfGeY5kfGmxbjDBd5NigQ>? NGPWflQyOCEQvF5CpC=> MVKyNEBucW5? NG[wfotqdmO{ZXHz[ZMh[0GPUDDhZ4N2dXWuYYTpc44> NGXpUVMzPTB4OUSxOy=>
SW480 MknUSpVv[3Srb36gRZN{[Xl? MWe0NOKh|ryP NFLtNlU1QMLiaB?= NVSwcplHTE2VTx?= MkLSZYN1cX[jdHXzJHBROkF? MVmyOFk6PzR3MR?=
HT-29  M4e1cmZ2dmO2aX;uJGF{e2G7 NX\LdZVpPDEEoN88US=> NIHwN3Q1QMLiaB?= NH;WSodFVVOR MXLhZ5RqfmG2ZYOgVHAzSQ>? M3i5bFI1QTl5NEWx
SW480 MljHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXi0PJV5PDEEoN88US=> NFHaRnUxNTd{IHi= MkfZSG1UVw>? Mk\KbY5pcWKrdIOgZ4VtdCCpcn;3eIghfGmvZTDk[ZBmdmSnboTsfS=> M3\4NlI1QTl5NEWx
HT-29  NXi0WYx5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkKzOFDDqM7:TR?= MkHONE04OiCq Mle3SG1UVw>? NGLY[m9qdmirYnn0d{Bk\WyuIHfyc5d1cCC2aX3lJIRmeGWwZHXueIx6 NVH6VlhOOjR7OUe0OVE>
SW480 NWHESY55TnWwY4Tpc44hSXO|YYm= MX:0NOKh|ryP NE\wR2g4KGR? M1HyXGROW09? MWLy[YR2[2W|IHPvcI9vd3OyaHXy[UBnd3KvYYTpc44h[2GyYXLpcIl1gcLi MoTkNlQ6QTd2NUG=
HT-29  MkfHSpVv[3Srb36gRZN{[Xl? MmfuOFDDqM7:TR?= NGq1Wnk4KGR? NFLUdlFFVVOR Mlf1doVlfWOnczDjc4xwdm:|cHjldoUh\m:{bXH0bY9vKGOjcHHibYxqfHoEoB?= NGT0T|czPDl7N{S1NS=>
SW480 NI\3R5pCeG:ydH;zbZMhSXO|YYm= NWXa[3RuPDEEoN88US=> NYLXbWR7PDkEoHi= NHXKXm5FVVOR M3rUfolv\HWlZYOgZY4h[WO2aY\heIlwdiCxZjDjZZNx[XOnIEOvOy=> M1XIflI1QTl5NEWx
HT-29  MWfBdI9xfG:|aYOgRZN{[Xl? MW[0NOKh|ryP MUW0POKhcA>? MWfEUXNQ MnqzbY5lfWOnczDhckBi[3SrdnH0bY9vKG:oIHPhd5Bie2ViMz:3 Mk\HNlQ6QTd2NUG=
SW480 M4[xWGFxd3C2b4Ppd{BCe3OjeR?= MWK0NOKh|ryP NEC3b3o1QMLiaB?= M3jrNWROW09? MkjVbY5lfWOnczDjbIFv\2W|IHnuJJRp\SCyaH;zdIhwenmuYYTpc44he3SjdIXzJI9nKFCSMlGgeIFz\2W2cx?= M3fMUFI1QTl5NEWx
HT-29  MmDYRZBweHSxc3nzJGF{e2G7 NXzsfW52PDEEoN88US=> MVm0POKhcA>? MXXEUXNQ NIHheJVqdmS3Y3XzJINp[W6pZYOgbY4hfGinIIDoc5NxcG:{eXzheIlwdiC|dHH0eZMhd2ZiUGCyRUB1[XKpZYTz M4q2VlI1QTl5NEWx

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
cleaved caspase-3 / caspase-3; 

PubMed: 30863177     


Toledo, NK-92, RL, and Farage cells were treated with 30 μM of forskolin for 48 hours, then the cells were harvested for Western blotting to assess the expression of total or cleaved caspase-3

cleaved caspase-9 / caspase-9 ; 

PubMed: 30863177     


Toledo, NK-92, RL, and Farage cells were treated with 30 μM of forskolin for 48 hours, then the cells were harvested for Western blotting to assess the expression of total or cleaved caspase-9.

β-catenin; 

PubMed: 30863177     


(A, B) The protein expression of cytoplasmic and nuclear β-catenin in Toledo and NK-92 cells in the presence of 0, 10, 20, or 40 μM forskolin was detected by Western blotting.

c-myc / Cyclin D1; 

PubMed: 30863177     


(C, D) Western blotting analysis of the total protein expression of c-myc and cyclin D1 influenced by 0, 10, 20, or 40 μM of forskolin in Toledo and NK-92 cells (n=3, *P<0.05, forskolin group vs control group).

pS6K1 / S6K1 / pCREB / CREB; 

PubMed: 31112131     


Forskolin inhibits mTORC1 in multiple cell lines. PC3, LNcap/AR, H1299, H1944, MDA-MB-231, MEF, HAP1, COS7, HeLa, MIA Paca-2, and PANC-1 were treated with or without 10 μM forskolin for 1 h and mTORC1 activity, CREB phosphorylation, and loading controls were analyzed.

p-JNK / JNK / P-p38 / p38; 

PubMed: 20070884     


L929 cells were pretreated with forskolin (10 μM) for various times as indicated, followed by stimulation with or without 10 ng/ml TNF-α for 15 min.

30863177 31112131 20070884
Immunofluorescence
5hmC; 

PubMed: 29239726     


Schwann cells were treated with forskolin (10 μM) for 3 hr followed by washout. 5hmC induction was detected at of 0, 3, and 24 hr time points following treatment. Cells continuously treated with forskolin (5, 10 μM) for 24 hr showed comparable 5hmC levels. Scale bar = 20 μm. 

Fe(II); 

PubMed: 29239726     


AMP (100 mM) treatment for 4 hr did not induce labile Fe(II) while AC activators (forskolin (100 mM), bicarbonate (50 mM)) and PDE inhibitors (caffeine (100 mM), IBMX (100 mM)) increased labile Fe(II) detected by Trx-Puro probes.

CYP17A1 / CYP21A2; 

PubMed: 25334044     


H295R cells were treated with 8-Br-cAMP or forskolin for 48 h under growth conditions, and fixed with 4% paraformaldehyde. (A) Immunostaining for CYP17A1. Red staining shows the anti-CYP17A1 antibody and blue staining shows DAPI (cell nuclei). (B) Immunostaining for CYP21A2. Red staining shows the anti-CYP21A2 antibody and blue staining shows DAPI (cell nuclei). Scale bars represent 100 µm.

29239726 25334044
Growth inhibition assay
Cell viability; 

PubMed: 30863177     


(C) MTT assay was conducted to test the cell growth after Toledo cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 1, 2, 3, 4, 5, or 6 days. (D) Toledo cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 48 hours, then MTT assay was used to evaluate the IC50 of forskolin. (F) MTT assay was conducted to test the cell growth after NK-92 cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 1, 2, 3, 4, 5, or 6 days. (G) NK-92 cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 48 hours, then MTT assay was used to evaluate the IC50of forskolin. 

30863177

Protocol

Solubility (25°C)

In vitro DMSO 82 mg/mL (199.75 mM)
Water Insoluble
Ethanol '37 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 410.5
Formula

C22H34O7

CAS No. 66575-29-9
Storage powder
in solvent
Synonyms Coleonol
Smiles CC(=O)OC1C(O)C2C(C)(C)CCC(O)C2(C)C3(O)C(=O)CC(C)(OC13C)C=C

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cAMP Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID