SQ22536

For research use only.

Catalog No.S8283 Synonyms: 9-(tetrahydrofuran-2-yl)-9h-purin-6-amine

9 publications

SQ22536 Chemical Structure

CAS No. 17318-31-9

SQ22536 is an inhibitor of adenylyl cyclase with an IC50 of 1.4 μM. It can inhibit PGE1-stimulated increases in cAMP levels in intact human platelets.

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Selleck's SQ22536 has been cited by 9 publications

2 Customer Reviews

  • Effects of AC inhibitor SQ22536 on A20 cell proliferation and expression of cAMP, Reelin, CDK5, IL-10 and caspase-3. (A) The expression level of cAMP in A20 cells treated with SQ22536 using ELISA assay. (B) The proliferation rate analysis of A20 cell treated with SQ22536 using CCK-8 assay. (C) Western blotting determined the expression levels of Reelin, CDK5, caspase-3 and IL-10 in A20 treated with SQ22536. **p<0.01.

    ONCOLOGY REPORTS, 2017, 37:3572-3580. SQ22536 purchased from Selleck.

    L-Arg-induced CCK and GIP secretion involves AC activation. (A to D) The porcine duodenal tissues were perfused with KHB (control group). After 30 min, 20 mM L-Arg was introduced into the superfusion system. After 60 min, the AC antagonist SQ22536 was introduced into the superfusion system and the samples were perfused for additional 30 min. Average CCK and GIP concentrations in perfusate samples obtained at t = 0 to 30 min, t = 30 to 60 min, and t = 60 to 90 min were calculated. Data are expressed as mean ± SEM (n = 8). Bars with different letters indicate significant difference at P < 0.05.

    J Food Sci, 2018, 83(9):2394-2401. SQ22536 purchased from Selleck.

Purity & Quality Control

Choose Selective cAMP Inhibitors

Biological Activity

Description SQ22536 is an inhibitor of adenylyl cyclase with an IC50 of 1.4 μM. It can inhibit PGE1-stimulated increases in cAMP levels in intact human platelets.
Targets
Adenylyl cyclase [1]
In vitro

SQ22536(250 µmol/L) attenuates the inhibitory effect of adenosine against ADP-induced platelet aggregation from 8±5 to 57±5%, respectively (p<0.001). SQ22536 also attenuates an increase of intraplatelet levels of cAMP by adenosine from 29±2 to 9±1 pmol/108 platelets (p<0.05). It has no effect on the platelet antiaggregant activity of inosine (1 to 4 mmol/L) and ADP-induced platelet aggregation[4].

In vivo SQ22536 abolishes the renal protective effects of liraglutide in KK/Ta-Akita mice. the amelioration of glomerular histopathological damage by liraglutide is eliminated in KK/Ta-Akita mice treated with liraglutide in combination with SQ22536. Renal cAMP does not increase after treatment with SQ22536. In a word, the beneficial actions of liraglutide for treatment of nephropathy are inhibited by the adenylate cyclase inhibitor SQ22536[5].

Protocol

Cell Research:[2]
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  • Cell lines: Human leukemic mast cell line(HMC-1) and human umbilical cord blood-derived mast cell(hCBMCs)
  • Concentrations: 0.1, 1, 10 mM
  • Incubation Time: 30 min
  • Method: HMC-1 cells and hCBMCs are plated in 48-well plates and serum-starved overnight. The next day, cells are preincubated with SQ22536 at the indicated concentrations for 30 min before stimulation with CRH (100 nM for HMC-1 or 1 μM for hCBMC) for 3 min in the presence or absence of SQ22536 in serum-free culture media. Cell lysates are then prepared and assayed for protein kinase A activity using ELISA.
    (Only for Reference)
Animal Research:[3]
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  • Animal Models: Male C57BL/6J mice
  • Dosages: 10 mg/kg
  • Administration: s.c.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 41 mg/mL (199.78 mM)
Water 41 mg/mL (199.78 mM)
Ethanol 41 mg/mL (199.78 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 205.22
Formula

C9H11N5O

CAS No. 17318-31-9
Storage powder
in solvent
Synonyms 9-(tetrahydrofuran-2-yl)-9h-purin-6-amine
Smiles C1CC(OC1)N2C=NC3=C(N=CN=C32)N

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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cAMP Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID