PACAP 6-38

For research use only.

Catalog No.S8416

PACAP 6-38 Chemical Structure

CAS No. 143748-18-9

PACAP 6-38 is a PACAP (pituitary adenylate cyclase-activating polypeptide) non-stimulating competitive antagonist with an IC50 value of 2 nM. It also acts as a functional CARTp antagonist in vivo.

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Biological Activity

Description PACAP 6-38 is a PACAP (pituitary adenylate cyclase-activating polypeptide) non-stimulating competitive antagonist with an IC50 value of 2 nM. It also acts as a functional CARTp antagonist in vivo.
Targets
CARTp [2] PACAP receptor [1]
2 nM
In vitro

PACAP 6-38 blocks the CARTp-induced phosphorylation of ERK in differentiated cells[2].

In vivo Intravesical administration of the PAC1 receptor antagonist, PACAP(6-38) (300 nM), significantly (p ≤ 0.01) increases intercontraction interval (2.0-fold) and void volume (2.5-fold) in NGF-OE mice. PACAP(6-38) has no effects on bladder function in WT mice. Intravesical administration of PACAP(6–38) (300 nM) significantly (p ≤ 0.01) reduces pelvic sensitivity in NGF-OE mice but is without effect in WT mice[4].

Protocol

Cell Research:[3]
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  • Cell lines: SK-N-MC neuroblastoma cells
  • Concentrations: 30 μM
  • Incubation Time: 4 h
  • Method: Cells are incubated for 4 h with 300 nM PACAP-38 in the absence or presence of the PACAP antagonist PACAP (6-38), medium is collected, and proteins are separated by SDS/PAGE and blotted onto PVDF membranes. For APPsα detection, membranes are probed with the antibody 6E10 followed by an anti-mouse antibody labeled with HRP, detection is performed with the ECL plus system.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Male Sprague-Dawley rats
  • Dosages: 0.3 nmol, 0.6 nmol, 3 nmol (1.5 Μl/340g)
  • Administration: i.c.v
    (Only for Reference)

Solubility (25°C)

In vitro Water 100 mg/mL (24.84 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 4024.74
Formula

C182H300N56O45S

CAS No. 143748-18-9
Storage powder
in solvent
Synonyms N/A

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cAMP Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID