Citalopram HBr

Catalog No.S4749 Synonyms: Nitalapram HBr, Prepram HBr, Bonitrile HBr, Lu 10-171 HBr

For research use only.

Citalopram HBr (Nitalapram HBr, Prepram HBr, Bonitrile HBr, Lu 10-171 HBr), an antidepressant, is an orally administered selective serotonin reuptake inhibitor (SSRI) with IC50 of 1.8 nM.

Citalopram HBr Chemical Structure

CAS No. 59729-32-7

Selleck's Citalopram HBr has been cited by 4 Publications

Purity & Quality Control

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Biological Activity

Description Citalopram HBr (Nitalapram HBr, Prepram HBr, Bonitrile HBr, Lu 10-171 HBr), an antidepressant, is an orally administered selective serotonin reuptake inhibitor (SSRI) with IC50 of 1.8 nM.
Targets
serotonin reuptake [1]
(In rat brain synaptosomal preparations)
1.8 nM
In vitro

Citalopram (Lu 10-171), a new bicyclic phthalane derivative, is an extremely potent inhibitor of neuronal serotonin (5-HT) uptake but has no effect on the uptake of noradrenaline(NA) and dopamine (DA) and no antagonistic activity towards 5-HT, histamine, gamma aminobutyric acid (GABA), acetylcholine, and morphine receptors. It is an extremely specific and potent inhibitor of neuronal 5-HT uptake. Uptake mechanisms for other transmitter amines are not influenced by the drug[1]. The SSRI citalopram has a greater effect on proliferation and a lesser effect on apoptotic activity. It affects cell cycle regulation by increasing proliferative potential and decreasing apoptotic activity in a site specific manner that may be indicative of hyperplasia. Citalopram alters FGF, MSX and TGFB expression in osteoblast cell culture[3].

In vivo Citalopram is devoid of cardiotoxic effects even when animals are exposed to concentrations far above the therapeutic level. In man citalopram is metabolized to compounds which are also potent 5-HT-uptake inhibitors without effect on noradrenaline(NA) uptake and which are found in lower concentrations than citalopram itself. Citalopram (1-16 mg/kg) stimulates the hind limb flexor reflex in the spinal rat. Citalopram potentiates 5-HT transmission~ possibly by producing very strong uptake inhibition without simultaneously blocking the post-synaptic 5-HT receptors[1].

Protocol (from reference)

Cell Research:[3]
  • Cell lines: MC3T3-E1 murine calvaria osteoprogenitor cells
  • Concentrations: 0.1 nM-100 μM
  • Incubation Time: 3 or 7 days
  • Method: Cells are cultured in alpha minimum Eagles medium supplemented with 1% penicillin/streptomycin, 10% fetal bovine serum and Amphotericin B. For control data, cells are cultured for 3 or 7 days with standard alpha proliferation media. For SSRI treatments, media is supplemented with citalopram eluted to serially diluted doses between 10−4 mol through 10−10 mol to achieve a dose response curve.
Animal Research:[2]
  • Animal Models: Spontaneously Hypertensive rats (SHRs), Lewis (LEW) rats, and Wistar-Kyoto (WKY) rats
  • Dosages: 1-10 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

DMSO 81 mg/mL warmed
(199.85 mM)
Water 81 mg/mL warmed
(199.85 mM)
Ethanol '81 mg/mL warmed

Chemical Information

Molecular Weight 405.3
Formula

C20H21FN2O.HBr

CAS No. 59729-32-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN(C)CCCC1(C2=C(CO1)C=C(C=C2)C#N)C3=CC=C(C=C3)F.Br

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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