Name: Puerarin
Brand: Selleck Chemicals
SKU: S2346
Availability: InStock
Rating: 5 (9 reviews)

Jump to

Quality Control

Batch: S234601 DMSO]86 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.80%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS/MSDS
Datasheet

99.80

Related Targets	Adrenergic Receptor AChR COX Calcium Channel Histamine Receptor Dopamine Receptor GABA Receptor TRP Channel Cholinesterase (ChE) GluR
Other 5-HT Receptor Inhibitors	Serotonin Hydrochloride (5-HT HCl) WAY-100635 Maleate SB269970 HCl Ketanserin BRL-15572 Dihydrochloride Nuciferine RS-127445 Flopropione Azacyclonol BRL 15572

Solubility

In vitro
Batch:

DMSO : 86 mg/mL (206.54 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.3mg/ml (10.33mM)	Taking the 1 mL working solution as an example, add 50 μL of 86 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.61mg/ml (1.47mM)	Taking the 1 mL working solution as an example, add 50 μL of 12.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	416.38	Formula	C₂₁H₂₀O₉	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	3681-99-0	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Kakonein			SMILES	C1=CC(=CC=C1C2=COC3=C(C2=O)C=CC(=C3C4C(C(C(C(O4)CO)O)O)O)O)O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro	Puerarin (Kakonein), an isoflavones found in the root of Radix puerariae, is a 5-HT2C receptor and benzodiazepine site antagonist. This compound is employed clinically to treat cardiovascular diseases in China. Another study showed that this chemical also possessed anti-cancer properties. It at 25 μM dose-dependently reduces HT-29 cellular growth with an increase of bax and decreases of c-myc and bcl-2.
In vivo	In hypercholesterolmic diet plus administration of puerarin (300 mg/kg/day, p.o.) rats, this compound markedly attenuated the increased total cholesterol induced by hypercholesterolmic diet in both serum and liver. LD50: Mice 738mg/kg (i.v.)
References	[1] https://pubmed.ncbi.nlm.nih.gov/12895679/ [2] https://pubmed.ncbi.nlm.nih.gov/16472823/ [3] https://pubmed.ncbi.nlm.nih.gov/16055262/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2025-05-21)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06968208	NOT_YET_RECRUITING	Metabolic Diseases; Obesity/Therapy	Ruijin Hospital	2025-08-01	PHASE2
NCT03676296	COMPLETED	Cardiovascular Disease Risk Factors	The University of Hong Kong	2018-09-05	PHASE2
NCT03099590	COMPLETED	Alcohol Drinking; Alcohol Use Disorder; Alcohol Abstinence	Mclean Hospital	2018-01-01	PHASE2
NCT02219191	UNKNOWN	Rheumatoid Arthritis	Chengdu PLA General Hospital	2014-08
NCT02264301	UNKNOWN	Rheumatoid Arthritis	Chengdu PLA General Hospital	2014-08
NCT02254655	COMPLETED	Rheumatoid Arthritis	Chengdu PLA General Hospital	2013-11	PHASE2

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Puerarin 5-HT Receptor antagonist

Chemical Structure

Molecular Weight: 416.38