Catalog No.S7563

For research use only.

AT13148 is an oral, ATP-competitive, multi-AGC kinase inhibitor with IC50 of 38 nM/402 nM/50 nM, 8 nM, 3 nM, and 6 nM/4 nM for Akt1/2/3, p70S6K, PKA, and ROCKI/II, respectively. Phase 1.

AT13148 Chemical Structure

CAS No. 1056901-62-2

Selleck's AT13148 has been cited by 10 Publications

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Biological Activity

Description AT13148 is an oral, ATP-competitive, multi-AGC kinase inhibitor with IC50 of 38 nM/402 nM/50 nM, 8 nM, 3 nM, and 6 nM/4 nM for Akt1/2/3, p70S6K, PKA, and ROCKI/II, respectively. Phase 1.
PKA [1]
(Cell-free assay)
ROCK2 [1]
(Cell-free assay)
ROCK1 [1]
(Cell-free assay)
p70S6K [1]
(Cell-free assay)
Akt1 [1]
(Cell-free assay)
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3 nM 4 nM 6 nM 8 nM 38 nM
In vitro

AT13148, as a multi-AGC kinase inhibitor, potently inhibits proliferation with GI50 values of 1.5 to 3.8 μM across a selected panel of cancer cell lines with deregulation of PI3K-AKT-mTOR or RAS-RAF pathways. In PTEN-deficient MES-SA cells, AT13148 also inhibits AKT and p70S6K signaling. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
insect cells NF[zU|BHfW6ldHnvckBie3OjeR?= MoPxOVAhdWmwcx?= MXTJcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiSHnzMZRi\2enZDDmeYxtNWynbnf0bEBpfW2jbjDy[YNwdWKrbnHueEBxPzCVNlug[ZhxemW|c3XkJIlvKFOyb3TvdJRmemFiZoL1[4lx\XKmYTDpcpNm[3RiY3XscJMhfXOrbnegRWtTWlKOU2PMVmEhe3Wkc4TyZZRmKGGwZDDhcoQhY2ejbX3hMVM{WC2DVGDdJIlv[3WkYYTl[EBnd3JiNUCgcYlveyCkeTDzZ4lvfGmubHH0bY9vKGOxdX70bY5oKG2ndHjv[EwhUUN3ME2wMlAyOs7:TT6= M3zVcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFM2PDR7NkCvK|5EcEWPQly8M4E,
U87MG MU\GeY5kfGmxbjDhd5NigQ>? MV2xJIhz NUfYdWdLUW6qaXLpeIlwdiCxZjDQT2IhcW5iaIXtZY4hXTh5TVegZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEe|a{Pi[ZRiKFOnckmgdIhwe3Cqb4L5cIF1cW:wIHnuZ5Vj[XSnZDDmc5IhOSCqcjDifUB1cW2nLYLld49tfmWmIH\seY9z\XOlZX7j[UBj[XOnZDDhd5NigSxiSVO1NF0yNjIQvF2u NVHjfmtwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzV2NEm2NE8oRkOqRV3CUFww[T5?
In vivo AT13148 (50 mg/kg p.o.) markedly inhibits the activity of both AKT and p70S6K AGC kinases, and subsequently exhibits marked antitumor effects in human tumor xenografts. [1]

Protocol (from reference)

Kinase Assay:


  • In vitro kinase assays:

    AT13148 is assayed against 40 kinases and the percentage inhibition at 10 μM of AT13148 is determined. Individual IC50 values are measured for selected kinases using ATP concentrations equivalent to the Km for each enzyme.

Cell Research:


  • Cell lines: MES-SA, MES-SA/Dx5, BT474, HCT-116, A549, PC3, SK-BR-3, MCF7, U87MG, MDA-MB-468, DU-145, and SK-OV-3 cell lines
  • Concentrations: ~10 μM
  • Incubation Time: 72 hours or 96 hours
  • Method:

    Cytotoxicity is determined using a 72 h Alamar Blue assay or a 96 h SRB assay.

Animal Research:


  • Animal Models: Athymic mice bearing MES-SA, BT474, or PC3 tumor xenografts
  • Dosages: ~50 mg/kg
  • Administration: p.o.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 313.78


CAS No. 1056901-62-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1C2=CNN=C2)C(CN)(C3=CC=C(C=C3)Cl)O

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01585701 Completed Biological: AT13148 Advanced Solid Tumours Cancer Research UK May 2012 Phase 1

(data from, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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