Perifosine (KRX-0401)

Catalog No.S1037 Synonyms: NSC639966, D21266

For research use only.

Perifosine (KRX-0401, NSC639966, D21266) is a novel Akt inhibitor with IC50 of 4.7 μM in MM.1S cells, targets pleckstrin homology domain of Akt. Phase 3.

Perifosine (KRX-0401) Chemical Structure

CAS No. 157716-52-4

Selleck's Perifosine (KRX-0401) has been cited by 161 publications

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Biological Activity

Description Perifosine (KRX-0401, NSC639966, D21266) is a novel Akt inhibitor with IC50 of 4.7 μM in MM.1S cells, targets pleckstrin homology domain of Akt. Phase 3.
Targets
Akt [1]
(MM.1S cells)
4.7 μM
In vitro

Perifosine develops anti-proliferative properties with IC50 of 0.6-8.9 μM in immortalized keratinocytes (HaCaT), and head and neck squamous carcinoma cells. [1] Perifosine strongly reduces phosphorylation levels of Akt and extracellular signal-regulated kinase (Erk) 1/2, induces cell cycle arrest in G1 and G2, and causes dose-dependent growth inhibition of mouse glial progenitors. [2] Perifosine (10 μM) completely inhibits the phosphorylation of Akt in MM.1S cells. [3] A recent study demonstrates Perifosine induces cell cycle arrest and apoptosis in human hepatocellular carcinoma cell lines by blockade of Akt phosphorylation. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HL-60 MlvjRZBweHSxc3nzJGF{e3OjeR?= MkPCNVDDqM7:TR?= NWjiO5JwOjRxNEigbC=> MULpcoR2[2W|IHHwc5B1d3OrczD0bY1mNWSncHXu[IVvfGy7 M1z1SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUOwPVYxLz5{MEGzNFk3ODxxYU6=
MOLM Mk[xRZBweHSxc3nzJGF{e3OjeR?= MXyxNOKh|ryP Ml[3NlQwPDhiaB?= MY\pcoR2[2W|IHHwc5B1d3OrczD0bY1mNWSncHXu[IVvfGy7 MmT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzM{C5OlAoRjJyMUOwPVYxRC:jPh?=
OCI MULBdI9xfG:|aYOgRZN{e2G7 M3TUT|ExyqEQvF2= MXSyOE81QCCq MkLvbY5lfWOnczDhdI9xfG:|aYOgeIlu\S2mZYDlcoRmdnSueR?= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF|MEm2NEc,OjBzM{C5OlA9N2F-
BJAB M1rsNGFxd3C2b4Ppd{BCe3O|YYm= MnvTNVDDqM7:TR?= MX2yOE81QCCq NGD6SmtqdmS3Y3XzJIFxd3C2b4Ppd{B1cW2nLXTldIVv\GWwdHz5 NYjDSWpjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxN|A6PjBpPkKwNVMxQTZyPD;hQi=>
MAVER NYS0V|lvSXCxcITvd4l{KEG|c4PhfS=> MWexNOKh|ryP MUWyOE81QCCq M4O3V4lv\HWlZYOgZZBweHSxc3nzJJRqdWVvZHXw[Y5l\W62bIm= NV7GfJpVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxN|A6PjBpPkKwNVMxQTZyPD;hQi=>
SKW6.4 M{\0eWFxd3C2b4Ppd{BCe3O|YYm= MkK4NVDDqM7:TR?= MY[yOE81QCCq M2OzVIlv\HWlZYOgZZBweHSxc3nzJJRqdWVvZHXw[Y5l\W62bIm= MojrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzM{C5OlAoRjJyMUOwPVYxRC:jPh?=
HL-60 M1;GTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX:yMVExyqEQvF2= M2DpS|Q5yqCq MkTEbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> NY\TcoV1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxN|A6PjBpPkKwNVMxQTZyPD;hQi=>
MOLM MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TPSVIuOTEEoN88US=> NIHCRlg1QMLiaB?= NGixeZJqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? Mof1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzM{C5OlAoRjJyMUOwPVYxRC:jPh?=
OCI MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\NXYRuOi1zMNMg{txO NFjLbJA1QMLiaB?= NYPXfHY5cW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF|MEm2NEc,OjBzM{C5OlA9N2F-
BJAB MlvoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rUPVIuOTEEoN88US=> M{HkR|Q5yqCq NFvTNnRqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? MofIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzM{C5OlAoRjJyMUOwPVYxRC:jPh?=
MAVER MofMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWqyMVExyqEQvF2= MVq0POKhcA>? M2jqdYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVz MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF|MEm2NEc,OjBzM{C5OlA9N2F-
SKW6.4 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYG5RmdHOi1zMNMg{txO Mn\uOFjDqGh? M3PhWYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVz MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF|MEm2NEc,OjBzM{C5OlA9N2F-
A2780cis  MlXqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;2bYgxNTJyIN88US=> NYP6V5hOPDhxN{KgbC=> M2DBZWlEPTEEoE5CpFbDqM7:bR?= Mm[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MEWyPVYoRjJyNEC1Nlk3RC:jPh?=
A2780 NES5T4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVGwMVIxKM7:TR?= M4j6S|Q5Nzd{IHi= M3zVXmlEPTEEoE5CpFPDqM7:bR?= NGjIZlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESwOVI6Pid-MkC0NFUzQTZ:L3G+
SKOV3  MmnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHXPZExNTRyIN88US=> Ml7MO|LDqGh? M1fUeGlEPTC-M{Cg{txONCCrbnjpZol1eyClZXzsJIdzd3e2aDDpckBiKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= MnTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MEWyPVYoRjJyNEC1Nlk3RC:jPh?=
PA-1  M1nwSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPLNE01OCEQvF2= MkTkO|LDqGh? NX75UY9KUUN3MI6yOUDPxE1uIHnubIljcXS|IHPlcIwh\3Kxd4ToJIlvKGFiZH;z[UBl\XCnbnTlcpQhdWGwbnXy M4H4VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEC1Nlk3Lz5{MESwOVI6PjxxYU6=
OAW-42 Mme5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fT[lAuPDBizszN Mn7JO|LDqGh? NH;2Vo5KSzVyfkGwJO69VSxiaX7obYJqfHNiY3XscEBoem:5dHigbY4h[SCmb4PlJIRmeGWwZHXueEBu[W6wZYK= NXzoXZR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0NFUzQTZpPkKwOFA2Ojl4PD;hQi=>
Bel-7402 NUPJVZNNSXCxcITvd4l{KEG|c4PhfS=> NFLaRpk2NzFyL{KwJO69VQ>? MYSyOE81QCCq M3Swfolv\HWlZYOgZZBweHSxc3nzJIF1KHSqZTDsc45oNXSrbXWg[Zhxd3O3cnW= NVjQOWRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4OFI1OjVpPkKwPFQzPDJ3PD;hQi=>
HepG2  NVX1V5NxSXCxcITvd4l{KEG|c4PhfS=> M4XYU|UwOTBxMkCg{txO M37ZcVI1NzR6IHi= NFW2fZlqdmS3Y3XzJIFxd3C2b4Ppd{BifCC2aHWgcI9v\y22aX3lJIV5eG:|dYLl MnTtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6NEK0NlUoRjJyOESyOFI2RC:jPh?=
Bel-7402 M4DaPWZ2dmO2aX;uJGF{e2G7 MkexOU8yOC9{MDFOwG0> NIjTU5EzPCCq NIjXTWdz\XO3bITzJIlvKHSqZTDhZ4N2dXWuYYTpc44hd2ZiY3XscEBvfW2kZYKgbY4hfGinIFeyM20heGijc3W= NUT1N49ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4OFI1OjVpPkKwPFQzPDJ3PD;hQi=>
HepG2  M3\nNGZ2dmO2aX;uJGF{e2G7 M1m4bVUwOTBxMkCg{txO MXWyOEBp NHXxXoxz\XO3bITzJIlvKHSqZTDhZ4N2dXWuYYTpc44hd2ZiY3XscEBvfW2kZYKgbY4hfGinIFeyM20heGijc3W= NELnT4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi0NlQzPSd-MkC4OFI1OjV:L3G+
Bel-7402 NHvG[4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXyV5l3PS9zMD:yNE81OCEQvF2= NYCxfWhuOjRxNEivO|IhcA>? MmHGbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYn;0bEB1cW2nIHHu[EBld3OnIHTldIVv\GWwdDDtZY5v\XJ? M4HLZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOESyOFI2Lz5{MEi0NlQzPTxxYU6=
HepG2  MmTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILsfI02NzFyL{KwM|QxKM7:TR?= NV3GfndlOjRxNEivO|IhcA>? MXvpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWViYX7kJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh2MkSyOUc,OjB6NEK0NlU9N2F-
CWR22RV1 MV\GeY5kfGmxbjDBd5NigQ>? NHTnWYk2KM7:TR?= MkXYNlQhcA>? NYXhTog2emWmdXPl[EBxcG:|cHjvdplt[XSrb36gc4YhSWu2IIPp[45q\mmlYX70cJk> M4XqOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEm2Nlc{Lz5{MUS5OlI4OzxxYU6=
CWR22RV1 MYHBdI9xfG:|aYOgRZN{e2G7 MljFNVAh|ryP NUjSc3FROjRiaB?= NUK0TmxY\W6qYX7j[ZMhemGmaXH0bY9vKGmwZIXj[YQh[XCxcITvd4l{ NFLkOlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS5OlI4Oyd-MkG0PVYzPzN:L3G+
CWR22RV1 NVP2eHR{S2WubDDWbYFjcWyrdImgRZN{[Xl? Mlj0NVAh|ryP MVGyOEBp NHvKVXFqdmO{ZXHz[ZMhe2Wwc3n0bZZqfHlib3[gbJVu[W5iQ2fSNlJTXjFiY3XscJMhfG9icnHkbYF1cW:w MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR7NkK3N{c,OjF2OU[yO|M9N2F-
A498 MmjWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVOwMVIxKM7:TR?= M37TT|czKGh? MkXPbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> MlTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4NESwOVAoRjJzNkS0NFUxRC:jPh?=
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CAKI-1 M4\Xbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmC3NE0zOCEQvF2= M3jjSFczKGh? MWHpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> MnnFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4NESwOVAoRjJzNkS0NFUxRC:jPh?=
786-O MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjad3UxNTJyIN88US=> MljXO|IhcA>? NIDYTYFqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? NHXRT|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[0OFA2OCd-MkG2OFQxPTB:L3G+
786-0 M2Daemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnWzNE01OCEQvF2= M3ywSVczKGh? Mnj6TWM2OH53IN88US=> NWrwbVJFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2OFQxPTBpPkKxOlQ1ODVyPD;hQi=>
769-P NX;yXmw6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFf6Oo0xNTRyIN88US=> M1qwcVczKGh? M1vxTGlEPTC-NT2xNEDPxE1? NE\KWIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[0OFA2OCd-MkG2OFQxPTB:L3G+
CAKI-1 NGTVOmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVOwMVQxKM7:TR?= MmnxO|IhcA>? Mn75TWM2OH5zMDFOwG0> MnWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4NESwOVAoRjJzNkS0NFUxRC:jPh?=
A498 M{DrVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIOzUpYxNTRyIN88US=> MXi3NkBp M2nJVIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVz MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ2NEC1NEc,OjF4NESwOVA9N2F-
HT-29  MYXDfZRwfG:6aXPpeJkhSXO|YYm= NX7RNWJDPSEQvF2= M{nrO|Q5KGh? NYrJPJh4\W6qYX7j[ZMheGGlbHn0ZZhmdCCrbnT1Z4VlKG:4YYLpZY4h[2GwY3XyJINmdGxiZHXheIjDqA>? MoW0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5N{WwOVQoRjJzN{e1NFU1RC:jPh?=
A2780 M4K2SGN6fG:2b4jpZ4l1gSCDc4PhfS=> NWfDNoJ1PSEQvF2= NYnCNoVvPDhiaB?= M3rXeIVvcGGwY3XzJJBi[2yrdHH4[YwhcW6mdXPl[EBwfmG{aXHuJINidmOncjDj[YxtKGSnYYToxsA> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd5NUC1OEc,OjF5N{WwOVQ9N2F-
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A549 NXLuXIYxTnWwY4Tpc44hSXO|YYm= NXXhb4RDOyEQvF2= NF\I[ng5KGh? MWjicI9kc3NibWTPVmMyNCCjbnSgSXJMNU2DUFugZYN1cX[jdHnvckBkd22kaX7l[EB4cXSqIF3FT{0yPjJ? NHrrbVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[5O|g6QSd-MkW2PVc5QTl:L3G+
H460 NXTRcVQ6TnWwY4Tpc44hSXO|YYm= MWmzJO69VQ>? MYW4JIg> NFHEbWtjdG:la4OgRWtVKGGldHn2ZZRqd25? M{\kOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nkm3PFk6Lz5{NU[5O|g6QTxxYU6=
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H460 NVfKXGJKSXCxcITvd4l{KEG|c4PhfS=> MoC1NU8{KM7:TR?= NGH0enk1QCCq MlH1bY5lfWOnczDhdI9xfG:|aYO= NXHQSolXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PVc5QTlpPkK1Olk4QDl7PD;hQi=>
A549 NWq4WZlbSXCxcITvd4l{KEG|c4PhfS=> NGDjXmkyNzNizszN M1XlS|Q5KGh? MWHpcoR2[2W|IHHwc5B1d3Orcx?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ7N{i5PUc,OjV4OUe4PVk9N2F-
H460 M{fkRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV[wMlMuOTBizszN NYX2S4JVOjRxN{KgbC=> MWfpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWViYX7kJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? M{jrNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nkm3PFk6Lz5{NU[5O|g6QTxxYU6=
A549 MmHwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTFNE4{NTFyIN88US=> MXeyOE84OiCq NYfBb2xzcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lJIFv\CCmb4PlJIRmeGWwZHXueEBu[W6wZYK= NVSzepZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PVc5QTlpPkK1Olk4QDl7PD;hQi=>
U-87 MG  M4\kdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2fWW|IxNzRyIN88US=> NHHvWJQzPC92ODDo NFnX[IhqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKHSrbXWgZY5lKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= M{nSUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUO0NlMzLz5{NUmzOFI{OjxxYU6=
HepG2 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXyyNE81OCEQvF2= MYSyOE81QCCq M2PaOolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZTDhcoQh\G:|ZTDk[ZBmdmSnboSgcYFvdmW{ M2nT[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUO0NlMzLz5{NUmzOFI{OjxxYU6=
U-87 MG  MUTGeY5kfGmxbjDBd5NigQ>? MYGyNOKh|ryP Mo\sOk8zPCCq M2jaWYlv[3KnYYPld{B1cGViYYX0c5Bp[WerYzDmcJV5KCCjdDC2JIghf2irbHWgbY5pcWKrdIOgeIhqeyCobIX4JIF1KDJ2aB?= NUjSUJRmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5N|QzOzJpPkK1PVM1OjN{PD;hQi=>
HepG2 MnjrSpVv[3Srb36gRZN{[Xl? NIPsWWIzOMLizszN M2rUPFYwOjRiaB?= NFjNNpll\WO{ZXHz[ZMhVEN|LVnJJIRm\3KjZHH0bY9vyqCocn;tJFYhcA>? NWHQOHlZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5N|QzOzJpPkK1PVM1OjN{PD;hQi=>
U-87 MG  MlHDSpVv[3Srb36gRZN{[Xl? MonwNlDDqM7:TR?= NYP5bWp[Pi9{NDDo MVfpcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iCOQ{OtTWkh[2:2cnXheIVlKHerdHigR3E> MljqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7M{SyN|IoRjJ3OUO0NlMzRC:jPh?=
HepG2 NHvMe3pHfW6ldHnvckBCe3OjeR?= NGrMWmwzOMLizszN M2nrTVYwOjRiaB?= MVnpcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iCOQ{OtTWkh[2:2cnXheIVlKHerdHigR3E> NVHYVG03RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5N|QzOzJpPkK1PVM1OjN{PD;hQi=>
U-87 MG  MX3GeY5kfGmxbjDBd5NigQ>? NHz4dXIzOMLizszN NYrnU3JqOjUEoHi= NEPxS5hqdmO{ZXHz[ZMh\G:3YnzlMY1mdWK{YX7lJIJwfW6mIIP0dpVkfHW{ZYO= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl|NEKzNkc,OjV7M{SyN|I9N2F-
HepG2 Mn62SpVv[3Srb36gRZN{[Xl? NXTPNJB3OjEEoN88US=> MonCNlTDqGh? MWHwdo9lfWOnczDhckBqdnSnboPlJIN6fG:ybHHzcYlkKH[jY4XvcIl7[XSrb36gZ49zemW|cH;u[Ilv\yC2bzDhJI5wfGGkbHWg[Ilt[XSjdHnvckBw\iC2aHWgSXIh[2m|dHXycpM> NFjjNFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUmzOFI{Oid-MkW5N|QzOzJ:L3G+
T24 BC  NW\sSWZMSXCxcITvd4l{KEG|c4PhfS=> NXj1XVlbOi53IN88US=> NGrVeYkzPCCq MlLtd4Vve2m2aYrld{BDSyClZXzsd{B1dyC|b4Lh[oVvcWJvaX7keYNm\CCjcH;weI91cWQEoB?= NWnLTJhURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPVc5PzNpPkK2NFk4QDd|PD;hQi=>
T24 BC  M1\tT2NmdGxiVnnhZoltcXS7IFHzd4F6 M3\kWFAvPS9zL{KuOUDPxE1? NXvNVWZ5OjRiaB?= NWLOUo1O\W6qYX7j[ZMhe2:{YX\lcoljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImg[IVkemWjc3W= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB7N{i3N{c,OjZyOUe4O|M9N2F-
T24 BC  Ml\BSpVv[3Srb36gRZN{[Xl? M4G0V|AvPS9zL{KuOUDPxE1? NULXNlB5OyCq MX3y[YR2[2W|IITo[UBj[XOjbDDDRkB1gXKxc3nu[UBxcG:|cHjvdplt[XSrb36gcIV3\Wy|IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NITQ[|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC5O|g4Oyd-Mk[wPVc5PzN:L3G+
RBL2H3 NF20VmlHfW6ldHnvckBie3OjeR?= Ml;lWI95cWOrdImgbY4hemG2IGLCUFJJOyClZXzsd{whVVSGPUK1{txO NWfVcHVFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOVM2PjVpPkKwNVU{PTZ3PD;hQi=>
PC3 NXvHe|JHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mk\RS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gVGM{KGOnbHzzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO|YYmsJGdKPTB;MD60OO69VQ>? M1rK[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUSzNVQyLz5{MUW0N|E1OTxxYU6=
NUGC3 M1nGe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHzNdZJIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBPXUeFMzDj[YxteyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvPTUQvF2= MorJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3NEOxOFEoRjJzNUSzNVQyRC:jPh?=
HCT15 NU\QZY1wT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXTvPZo4T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWMUWgZ4VtdHNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiR1m1NF0yNjJ3zszN M{frd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUSzNVQyLz5{MUW0N|E1OTxxYU6=
MDA-MB-231 NUS0[XZuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV7hR|FuT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5MEBIUTVyPUKuPFbPxE1? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV2M{G0NUc,OjF3NEOxOFE9N2F-
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ACHN NXXaPZJpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUDXTHhmT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hSUOKTjDj[YxteyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVQvPTcQvF2= MmnwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3NEOxOFEoRjJzNUSzNVQyRC:jPh?=
A549 NHLXcFJHfW6ldHnvckBie3OjeR?= MUGzNEBucW6| MoHzTY5pcWKrdHnvckBw\iCDa4SgdIhwe3Cqb4L5cIF1cW:wIHnuJIlve3WuaX6td5RqdXWuYYTl[EBpfW2jbjDBOVQ6KGOnbHzzJJRz\WG2ZXSgNkBpenNiYnXmc5JmKGmwc4XsbY4he3SrbYXsZZRqd25ibXXhd5Vz\WRiYX\0[ZIhOzBibXnud{BjgSCHTFnTRUwhUUN3ME21MlPPxE1? M1zEXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUO4N|A6Lz5{MkGzPFMxQTxxYU6=
A549 NXjIVG5US3m2b4TvfIlkcXS7IHHzd4F6 MYiyOEBpenN? Mnn1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjCyOEBpenNiYomgSmFEWyCjbnHsfZNqeyxiSVO1NF04|ryP MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF|OEOwPUc,OjJzM{izNFk9N2F-
KATO III NEXYcJVEgXSxdH;4bYNqfHliYYPzZZk> MVKyOEBpenN? M{\1UWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtCXE9iSVnJJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCIQVPTJIFv[Wy7c3nzMEBKSzVyPUGyMljPxE1? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF|OEOwPUc,OjJzM{izNFk9N2F-
MCF7 MmezR5l1d3SxeHnjbZR6KGG|c3H5 NFK3OIgzPCCqcoO= NIfSXJdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFI1KGi{czDifUBHSUOVIHHuZYx6e2m|LDDJR|UxRTF|LkROwG0> NES4S2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGzPFMxQSd-MkKxN|g{ODl:L3G+
PC3 M2ThZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MljRS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gVGM{KGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0xNjR2zszN NXHNRoVWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOlYyQDFpPkKzNlY3OThzPD;hQi=>
NUGC3 NHjYcI5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{\wWGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG5WT0N|IHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD1yLkW0{txO NUjiRlhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOlYyQDFpPkKzNlY3OThzPD;hQi=>
HCT15 NYiyRmZGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYPHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyPSClZXzsd{BjgSCVUlKgZZN{[XluIFfJOVA:OS5{Nd88US=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ4NkG4NUc,OjN{Nk[xPFE9N2F-
MDA-MB-231 MmC1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFXGR5lIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0zNjh4zszN MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ4NkG4NUc,OjN{Nk[xPFE9N2F-
NCI-H23 MmrjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX\Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDOR2kuUDJ|IHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD12LkKx{txO MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ4NkG4NUc,OjN{Nk[xPFE9N2F-
ACHN NF\ZSVdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHfWVXRIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBCS0iQIHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD12LkW2{txO MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ4NkG4NUc,OjN{Nk[xPFE9N2F-
A549 M4nYUGZ2dmO2aX;uJIF{e2G7 MXiyJIhzew>? NYm3SYI4UW6qaXLpeIlwdiCxZjDBb5QheGixc4Doc5J6dGG2aX;uJIlvKGi3bXHuJIlve3WuaX6td5RqdXWuYYTl[EBCPTR7IHPlcIx{KGmwY4XiZZRm\CCob4KgNkBpenNicILpc5IhfG9iaX7zeYxqdi2rbnT1Z5Rqd25ibXXhd5Vz\WRiYX\0[ZIhOzBibXnud{BjgSCHTFnTRUwhUUN3ME21MlPPxE1? M2TpN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEG1NFg{Lz5{M{SxOVA5OzxxYU6=
A549 Mny3R5l1d3SxeHnjbZR6KGG|c3H5 MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIJ6KG[ub4egZ5l1d22ndILpZ{BidmGueYPpd{whUUN3ME23{txO NFTOSWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxOVA5Oyd-MkO0NVUxQDN:L3G+
KATO III NFLPfmxEgXSxdH;4bYNqfHliYYPzZZk> NXnDVFNtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0GWTzDJTWkh[2WubIOgZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nzMEBKSzVyPUGyMljPxE1? M2nWO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEG1NFg{Lz5{M{SxOVA5OzxxYU6=
MCF7 M1;5OmN6fG:2b4jpZ4l1gSCjc4PhfS=> NGfRNZVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGK7IH\sc5ch[3m2b33leJJq[yCjbnHsfZNqeyxiSVO1NF0yOy5|zszN M4[4VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEG1NFg{Lz5{M{SxOVA5OzxxYU6=
PC3 Mn7qRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MonuRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCSQ{OgZ4VtdHNiYomgV3JDKGG|c3H5MEBIUTVyPUCuOFTPxE1? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV4N{m1NEc,OjN3Nke5OVA9N2F-
NUGC3 MmPZRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MUTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6XR1OzJINmdGy|IHL5JHNTSiCjc4PhfUwhT0l3ME2wMlU1|ryP NWTLbIRjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1Olc6PTBpPkKzOVY4QTVyPD;hQi=>
HCT15 Ml21RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NIW5TWhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFE2KGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0yNjJ3zszN MnfiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3Nke5OVAoRjJ|NU[3PVUxRC:jPh?=
MDA-MB-231 MoHkRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NXHMWHpRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHL5JHNTSiCjc4PhfUwhT0l3ME2yMlg3|ryP M{WwR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NU[3PVUxLz5{M{W2O|k2ODxxYU6=
NCI-H23 M3fzSmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M4jEXmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJMWgzOyClZXzsd{BjgSCVUlKgZZN{[XluIFfJOVA:PC5{Md88US=> M4LaWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NU[3PVUxLz5{M{W2O|k2ODxxYU6=
ACHN M{DvXmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M2PkUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQVPIUkBk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9OE42Ps7:TR?= Ml;VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3Nke5OVAoRjJ|NU[3PVUxRC:jPh?=
PC3 NIfxZ2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2fC[lQ5KGi{cx?= NF;BVm5Iem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBRSzNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3ME2wMlQ1|ryP M2PIfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm1O|U6Lz5{NEC5OVc2QTxxYU6=
NUGC3 MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYG0PEBpenN? MVHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDOWWdEOyClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBIUTVyPUCuOVTPxE1? MlHBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUW3OVkoRjJ2MEm1O|U6RC:jPh?=
HCT15 MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NILhc3A1QCCqcoO= MYrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyPSClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBIUTVyPUGuNlXPxE1? MlvHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUW3OVkoRjJ2MEm1O|U6RC:jPh?=
MDA-MB-231 MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF[3bGw1QCCqcoO= MmPLS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTJwOEdOwG0> NH;0[Xo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5OVc2QSd-MkSwPVU4PTl:L3G+
NCI-H23 MnS0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYS0PEBpenN? MWLHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDOR2kuUDJ|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEeLNUC9OE4zOc7:TR?= NUfFPVdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVU4PTlpPkK0NFk2PzV7PD;hQi=>
ACHN M3nnNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mo\aOFghcHK| M2PnZmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGFEUE5iY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3ME20MlU3|ryP NYjx[YNzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVU4PTlpPkK0NFk2PzV7PD;hQi=>
A549 NILQZ|lEgXSxdH;4bYNqfHliYYPzZZk> MmG4NlQhfG9iN{KgbJJ{ MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEK0JJRwKDd{IHjyd{BjgSCqYXXtc4N6fG:vZYTyfUwhUUN3ME20MlE4|ryP MoP4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC2NlAoRjJ2OUCwOlIxRC:jPh?=
Rosetta cells NEjq[JhHfW6ldHnvckBie3OjeR?= MmfkTY5pcWKrdHnvckBw\iC5aXzkMZR6eGViaIXtZY4hWDN6YXzwbIEhVUGSSzDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBTd3OndIThJINmdGy|LDDJR|UxRTFwMt88US=> M4TTSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzMke0N|E3Lz5|MUK3OFMyPjxxYU6=
Assay
Methods Test Index PMID
Western blot p-AKT / AKT / p-S6K1 / S6K1 ; PARP ; p-mTOR / mTOR / Raptor / Rictor / p-p70S6K / p70S6K / p-4EBP1 / 4EBP1 / c-Myc / Cyclin D1 ; p-PDK1 / p-GSK3α/β / p-S6R 25519148 19920197 17761832
Growth inhibition assay Cell viability 28332584
In vivo Perifosine combining with temozolomide reduces tumor proliferation (a PDGF-driven gliomagenesis) in vivo. The results indicate that Perifosine is an effective drug in gliomas in which Akt and Ras-Erk 1/2 pathways are frequently activated, and may be new candidate for glima treatment in the clinic. [2] Both oral daily and weekly administration of Perifosine significantly reduce human MM tumor growth and increase survival, compared with control animals treated with PBS vehicle only. [3] Perifosine induces thrombocytosis and leukocytosis and increases myelopoiesis in murine marrow and spleen, whereas it causes apoptosis in myeloma xenografts. [5]

Protocol (from reference)

Kinase Assay:[3]
  • Akt kinase assay:

    MM.1S cells are cultured in the presence or absence of perifosine (5 μM, 6 hours) and then stimulated with IL-6 (20 ng/mL, 10 minutes). In vitro akt kinase assay is then carried out using the Akt Kinase Assay Kit.

Cell Research:[2]
  • Cell lines: Human glioma cell lines
  • Concentrations: 0, 15, 30 and 45 μM
  • Incubation Time: 48 hours
  • Method: Cells are incubated in the medium with 10% FCS for 48 hours with indicated concentration of Periosine. Cell viability is determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (Cell Proliferation Kit I; Roche). The absorbance at 590 nm is recorded using the 96-well plate reader.
Animal Research:[3]
  • Animal Models: MM.1S MM cells are inoculated subcutaneously in the right flank of Beige-nude-xid (BNX) mice (5 to 6 weeks old).
  • Dosages: 250 mg/kg/wk or 36 mg/kg/d
  • Administration: Oral gavage

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
water
For best results, use promptly after mixing.

8mg/mL

Chemical Information

Molecular Weight 461.66
Formula

C25H52NO4P

CAS No. 157716-52-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCCCCCCCCCCCCCCCCCOP(=O)([O-])OC1CC[N+](CC1)(C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01224730 Completed Drug: perifosine Cancer AEterna Zentaris January 24 2012 Phase 1
NCT01049841 Completed Drug: perifosine + temsirolimus Pediatric Solid Tumors Memorial Sloan Kettering Cancer Center|University of Wisconsin Madison|Duke University|NATL COMP CA NETWORK|Pfizer|AEterna Zentaris January 2010 Phase 1
NCT01048580 Completed Drug: Perifosine|Drug: Capecitabine Colon Cancer AEterna Zentaris|SCRI Development Innovations LLC October 2009 Phase 1
NCT00776867 Completed Drug: perifosine Solid Tumors Memorial Sloan Kettering Cancer Center|University of Wisconsin Madison|Duke University|AEterna Zentaris October 2008 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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