SC66

Catalog No.S5313

SC66 Chemical Structure

Molecular Weight(MW): 276.33

SC66 is an allosteric inhibitor which displays a dual-inhibitory function toward AKT activity with IC50 values of 0.77, 2.85 and 0.47 μg/ml in HepG2, Huh7 and Hep3B cells after 72 h treatment, respectively.

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Biological Activity

Description SC66 is an allosteric inhibitor which displays a dual-inhibitory function toward AKT activity with IC50 values of 0.77, 2.85 and 0.47 μg/ml in HepG2, Huh7 and Hep3B cells after 72 h treatment, respectively.
Targets
Akt [1]
()
In vitro

SC66 reduces cell viability in a dose- and time-dependent manner, inhibits colony formation and induces apoptosis in HCC cells. SC66 treatment leads to a reduction in total and phospho-AKT levels. This is associated with alterations in cytoskeleton organization, a reduction in expression levels of E-cadherin, β-catenin and phospho-FAK, together with up-regulation of Snail protein levels. In addition, SC66 induces the production of reactive oxygen species (ROS) and DNA damage. SC66 affects AKT/mTOR signaling in HCC cell lines[1].

In vivo In the mouse xenograft tumor model of Hep3B cells, SC66 treatment significantly reduces tumor volume to 37% on day 17 of treatment when compared with tumors in the untreated group. The inhibition of cell growth correlates with a reduction in phospho-AKT levels in the tumors of animals treated with SC66[1].

Protocol

Cell Research:[1]
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  • Cell lines: The human hepatocarcinoma cell lines HepG2, Huh7, PLC/PRF/5, Hep3B and HA22T/VGH cells
  • Concentrations: 2 and 4 μg/ml
  • Incubation Time: 1, 3 and 6 hours
  • Method: Caspase activity assays: Cells (2 × 104/well) are treated with 2 and 4 μg/ml SC66 and after 1, 3 and 6 hours the levels of caspase3/7 activities in the cells are measured by the Caspase-Glo® 3/7 Assay. Results are expressed as relative luminescence units (RLU). Values are the mean ± SD of two separate experiments, each performed in duplicate.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Male nude athymic mice (Fox1 nu/nu) aged 4 weeks inoculated with Hep3B cells at the right flank
  • Formulation: suspended in DMSO, further diluted in a solution of 25% ethanol
  • Dosages: 5 and 25 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 55 mg/mL (199.03 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 276.33
Formula

C18H16N2O

CAS No. 871361-88-5
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID