MK-2206 2HCl

Catalog No.S1078

MK-2206 2HCl Chemical Structure

Molecular Weight(MW): 480.39

MK-2206 2HCl is a highly selective inhibitor of Akt1/2/3 with IC50 of 8 nM/12 nM/65 nM in cell-free assays, respectively; no inhibitory activities against 250 other protein kinases observed. Phase 2.

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Cited by 187 Publications

21 Customer Reviews

  • Inhibition of AKT signaling abolishes MKK4 phosphorylation on Ser78 in injured axons. Cultures of sensory neurons were treated with 5 µM MK-2206 or 5 µM GDC-0068 for 1 hr prior to axotomy. Axonal proteins harvested at indicated time points after axotomy were subjected to immunoblot analysis.

    Cell, 2015, 160(1-2): 161-76 . MK-2206 2HCl purchased from Selleck.

    j,k, Gene expression ofMYOCD (j) and ACTA2 ( SMA, k) after applying inhibitors of key components involved in the DDR2 downstream signalling pathway to HSCs cultured within 3D collagen matrix subjected to stretching (ST) (n=3, one-way ANOVA, **P=0.0036, ****P<0.0001). l,m, Expression of SMA was significantly reduced after treatment with related inhibitors in early-stage FμNs. (n=4, one-way ANOVA, ***P=0.001, ****P<0.0001). AKT-i: MK-2206

    Nature Materials, 2017, 16:1252-1261.. MK-2206 2HCl purchased from Selleck.

  • The PI3K or AKT inhibition does not restore sensitivity to WZ4002 in PC9 WZR. PC9GR4 or WZR10 cells were treated with WZ4002 alone at the indicated concentrations or in combination with the AKT inhibitor MK-2206 (1 uM). MK-2206 effectively inhibited AKT in both cells.

    Cancer Discov 2012 2, 934-47. MK-2206 2HCl purchased from Selleck.

    Cancer Cell 2013 24, 766-76. MK-2206 2HCl purchased from Selleck.

  • VE-cadherin-induced Akt activation mediates YAP phosphorylation and translocation in ECs. HUVECs were starved for 1h and treated with thrombin (1U) for 1h. Total cell lysates were probed with anti-pAkt, Akt or b-actin antibody. The representative blots of three independent experiments are depicted, and the normalized values for p-Akt are shown. HUVECs were cultured and starved as described as in d and incubated for 8h in complete medium with the Akt inhibitor, MK-2206 (1 uM). pAkt, Akt, pYAP and YAP were detected by western blotting using specific antibodies.

    Nat Commun 2015 6:6943. MK-2206 2HCl purchased from Selleck.

    Rap1b negatively regulates neutrophil transcellular migration by limiting PI3K-Akt signaling. (A-D) Effect of Akt inhibitor MK2206 (2 uM), Src inhibitor PP2 (10 uM), or vehicle control (DMSO) on WT or Rap1b-/- neutrophil functions. (A) Percentage of neutrophil transendothelial migration in 3D migration model. (B) ECM degradation assessed on Oregon green-labeled gelatin matrix; (left) representative images on (bar, 10 um) and (right) bar graph is percentage of matrix degradation. (C) Percentage of cells forming multiple protrusions. (D) Percentage of neutrophils present at junction of activated bEND.3 in 3D migration assay. Mean ?SD; n = 3 independent experiments. **, P < 0.01; ***, P < 0.001; NS, not significant using unpaired Student's t test).

    J Exp Med 2014 211(9), 1741-58. MK-2206 2HCl purchased from Selleck.

  • Inhibitors of AKT or ERK overcome SDF-1a-mediated resistance to ibrutinib-triggered PARP and caspase 3 cleavage in CXCR4S338X-expressing BCWM.1 cells. CXCR4S338X-expressing WM cells were treated with ibrutinib (0.5 uM) alone or in the presence of SDF-1a (20 nM) and/or the AKT inhibitors MK-2206 (0.5 uM) and AZD-5363 (0.5 uM); or the MEK inhibitors AS-703026 (0.25 uM), AZD-6244 (0.5 uM) and UO126 (5.0 uM). (a) Immunoblotting results for phosphoAKT (S473) and phospho-ERK (T202/Y204) in CXCR4S338X-expressing BCWM.1 cells pretreated with ibrutinib with and without AKT or ERK inhibitors, then subjected to SDF-1a stimulation for 2 min. The inhibitory effect of AZD-5363 on AKT, which is known to paradoxically hyper-phosphorylate pAKT(S473) was confirmed by inhibition of the phospho-activity for the downstream AKT targets glycogen synthase kinase 3b and pS6. (b) Immunoblotting results for cleaved PARP and cleaved caspase 3 in CXCR4S338X-expressing BCWM.1 cells treated with ibrutinib and/or AKT or ERK inhibitors for 6 h at IC50 doses. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

    Leukemia 2015 29(1), 169-76. MK-2206 2HCl purchased from Selleck.

    Induction of apoptosis positively correlates with FOXO3a protein levels and phosphorylation. (a) Treatment of 11 cell lines seeded in 6-well plates with 8 μM MK-2206 or DMSO for 48 hours. Nuclei were harvested for PI staining and FACS analysis and the differences between drug and no drug are displayed as in Figure b. Both conditions were performed in triplo for each cell line. Below the FACS data is a we stern blot of cell lysates from this panel treated with 8 μM MK-2206 or DMSO and harvested at 24 hours. Each cell line name corresponds to the two western lanes below it and the four cell cycle bars above it.

    Cancer Res 2013 73, 2189-98. MK-2206 2HCl purchased from Selleck.

  • Mean IL-8 concentrations determined by ELISA of the supernatants of HeLa cells infected with wild-type Salmonella. Kinase inhibitors are indicated on the x axis, and the target families of the inhibitors are indicated above each column. CEC, chelerythrine; Pim Inh, Pim-1 inhibitor 2. Inhibitors that significantly affected IL-8 production relative to the control (P < 0.05, Bonferroni post hoc test from one-way ANOVA) are indicated with an asterisk. Relative cell viability is also shown, as determined by reduction of XTT by viable cells. A450, absorbance at 450 nm.

    Sci Signal 2011 4, rs9. MK-2206 2HCl purchased from Selleck.

    Reducing cellular levels of PtdIns5P by overexpression of PIP4Kα inhibits clonogenic growth of U2OS but enhances cell survival in response to H2O2 stimulation. A) U2OS cells (1000) were plated, allowed to attach overnight, and then treated with DMSO (control), PI-103 (0.5 μM), U0126 (5 μM), or SB203580 (5 μM) for 9 d. Cell colonies were stained using crystal violet. Dried plates were scanned. Representative results are shown. B) U2OS cells (1000) were plated, allowed to attach overnight, and then treated with DMSO (control), MK-2206 (1 μM), triciribine (1 μM), KU0063794 (1 μM), or PI-103 (0.5 μM) for 9 d. Cell colonies were stained using crystal violet. Dried plates were scanned. Representative results are shown.

    FASEB J 2013 27, 1644-56. MK-2206 2HCl purchased from Selleck.

  • A. Western blot analysis of pAKT activation in ovarian cancer cell lines treated with exogenous IGF-1. B. Activation of pAKT by IGF-1 in low-grade ovarian cancer cell lines was blocked by the AKT inhibitor MK-2206 in a dose-dependent manner.

    Gynecol Oncol 2011 123, 13-8. MK-2206 2HCl purchased from Selleck.

    Comparative effects of inhibitors by immunofluorescence microscopy study. Confluent HC11 cells were grown on poly-L-lysine-coated glass coverslips (immunofluorescence) and on plastic plates (biochemical control) and then treated with inhibitors according to the standard procedure. Upper part: the biochemical action of the inhibitors was tested to validate the immunofluorescence results. Cellular proteins were analyzed by SDS-PAGE and the immunoblots were successively probed with anti-ADRP, anti-β-casein, and anti- β-actin antibodies and their respective HRP-conjugated secondary antibodies. Each experimental condition was performed in duplicate. Lower part: cells were fixed, permeabilized and subjected to immunofluorescence microscopy using antiserum against ADRP and TRITC-conjugated secondary antibody (red).

    Biochim Biophys Acta 2012 1823, 987-96. MK-2206 2HCl purchased from Selleck.

  • Confocal microscopy images of NO formation. DAF 2 DA-loaded washed platelets (1.0109 platelets/mL) were preincubated at 378C with saline (A), and then stimulated for 1min with 0.1 (B), 1.0 (C) or 10 (D) μM AEA. In Panel (E-F-G) washed platelets were preincubated with 1 μM SR1 (E), 1 Mm MK2206 (F) or 20 μM LY294002 (G), and then stimulated for 1min with 1.0 μM AEA. In panel (H) is reported the effect of 5 mg/mL collagen, used as a positive control. All the experiments were carried out in the presence of 100 μM L-arginine. NO formation was visualized by confocal microscropy as detailed in Methods.

     

     

    J Cell Biochem 2011 112, 924–932. MK-2206 2HCl purchased from Selleck.

    The AEA effect on eNOS phosphorylation. Washed platelets (1.0109 platelets/mL), preincubated at 378C with saline, 1 μM SR1, 1 μM SR2, 20 μM LY294002, 1 μM MK2206, 1.0 μM EGTA, or 30 μM BAPTA/AM, were stimulated for 1 min with 1.0 μM AEA. At the end of incubation suitable aliquots were immunoblotted with anti-p-eNOSser1177 as detailed in Methods.Blots are representative of five independent experiments.

     

     

    J Cell Biochem 2011 112, 924–932. MK-2206 2HCl purchased from Selleck.

  • Effect of selected agents on NOx and cGMP formation induced by AEA. Washed platelets (1.0109 platelets/mL), prewarmed at 378C with saline or 1mM SR1, 1 μM SR2, 20 μM URB597 (URB), 1 μM MK2206 (MK) or 20 μM LY294002 (LY), were incubated for 1min with 100 μM L-arginine in the presence of 1.0 μM AEA. NOx (panel A) and cGMP (panel B) content were determined as detailed in Methods. Each bar represents the meanSD of five independent experiments carried out in triplicate. Student,s t-test: P <0.0001 versus none;P <0.0005; P <0.005 versus AEA

     

     

    J Cell Biochem 2011 112, 924–932. MK-2206 2HCl purchased from Selleck.

    C.U87MG cells were treated with vehicle or 1 μM MK-2206 for 1 hr, and then irradiated with 6 Gy. Total cell lysate was harvested 1 hr after IR and subjected to Western blot analysis with the indicated antibody. Cells without IR treatment were used as a control.

    D. Cells were treated with vehicle (control) or 1 μM MK-2206 for 1 hr, then irradiated with indicated dosage. 4 hr after IR, cells were fed with drug-free medium, and incubated for another 20 hr at 37°C, after which they were trypsinized and seeded for clonogenic survival assay. Colony-forming efficiency was determined 14 d later.
     

     

    Radiat Oncol 2009 4, 43. MK-2206 2HCl purchased from Selleck.

  • (B) Dose-response curves for DIPG 4 and SF7761 cells treated with the small molecule AKT inhibitor MK-2206 (error bars represent SEM).

    Oncol Rep, 2018, 39(2):455-464. MK-2206 2HCl purchased from Selleck.

    MK-2206 2HCL inhibited the sorafenib-induced PI3K/mTOR pathway activation and enhanced the cytotoxic effects of sorafenib in resistant cell lines. (A) HUH-7 hepatoma cells treated with sorafenib (5 μM) for 24 h with or without pretreatment with specific kinase inhibitors (MK-2206 2HCL, 15 μM). The expressions of p-AKT and cleaved PARP were revealed by Western blotting. (B) SK-HEP-1 hepatoma cells treated with sorafenib (5 μM) for 24 h with or without pretreatment with specific kinase inhibitors (MK-2206 2HCL, 15 μM). The expressions of p-AKT and cleaved RARP were revealed by Western blotting. (C) HUH-7 hepatoma cells treated with sorafenib (5 μM) with or without the kinase inhibitors for 24 h. The proportions of apoptotic cells were evaluated by annexin V labeling. (D) SK-HEP-1 hepatoma cells treated with sorafenib (5 μM) with or without the kinase inhibitors for 24 h. The proportions of apoptotic cells were evaluated by annexin V labeling. (*P < 0.05, HUH-7, SK-HEP-1 are control groups, R-HUH-7, R-SK-HEP-1 are resistant groups).

    J Surg Res, 2016, 206(2):371-379. MK-2206 2HCl purchased from Selleck.

  • IC50 values for an Akt inhibitor (MK-2206) and a MEK inhibitor (U0126) in a panel of mouse and human anaplastic (ATC) and follicular (FTC) carcinoma cell lines. On the bottom, a Western blotting showing the effect on the activation of Akt and ERK1/2 of one hour exposure to these inhibitors (MK-2206: 500 nM, U0126: 10 μM).

    Oncotarget 2011 2, 1109-26. MK-2206 2HCl purchased from Selleck.

    After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of MK-2206 for 3h,followed by 15-minute stimolation of 100ng/ml EGF.

     
     

     

    Dr. Zhang of Tianjin Medical University. MK-2206 2HCl purchased from Selleck.

  •  

    PI3K pathway signaling in GDC-0941 and MK-2206 treated MCF-7 derivatives with PIK3CA or  AKT1 mutations. Cells were grown in medium containing 5% FBS and treated with vehicle or increasing concentrations of GDC-0941 (0 nM, 50 nM, 100 nM, and 400 nM) or MK-2206 (0 nM, 100 nM, 250 nM, 1000 nM). After 24 hours of drug treatment, lysates were prepared and equal amounts of protein were load ed onto SDS-PAGE gels and blotted with the indicated antibodies.

    MK-2206 2HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Akt Inhibitors

Biological Activity

Description MK-2206 2HCl is a highly selective inhibitor of Akt1/2/3 with IC50 of 8 nM/12 nM/65 nM in cell-free assays, respectively; no inhibitory activities against 250 other protein kinases observed. Phase 2.
Features The first allosteric small molecule inhibitor of Akt to enter clinical development.
Targets
Akt1 [1]
(Cell-free assay)
Akt2 [1]
(Cell-free assay)
Akt3 [1]
(Cell-free assay)
8 nM 12 nM 65 nM
In vitro

MK-2206 is an allosteric inhibitor and is activated by the pleckstrin homology domain. MK-2206 inhibits auto-phosphorylation of both Akt T308 and S473. MK-2206 also prevents Akt-mediated phosphorylation of downstream signaling molecules, including TSC2, PRAS40 and ribosomal S6 proteins. [1] MK-2206 inhibits Ras wild-type (WT) cell lines (A431, HCC827, and NCI-H292) more potently when compared to Ras-mutant cell lines (NCI-H358, NCI-H23, NCI-H1299, and Calu-6). MK-2206 also shows synergistic responses in combination with cytotoxic agents such as erlotinib or lapatinib in lung NCI-H460 or ovarian A2780 tumor cells. [2] MK-2206 or siRNA-mediated Akt inhibition strongly activates autophagy in human glioma cells. However, eukaryotic elongation factor-2 (eEF-2) silencing suppresses MK-2206-induced-autophagy, with a promotion of apoptotic cell death. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H292 M{DXU2N6fG:2b4jpZ{BCe3OjeR?= M2LtSFMh|ryP NFHofYY4OiCq NYrsNYM4TE2VTx?= M{nTW2lvcGmkaYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:w NGDaTnIzODV5MUC2PS=>
A431 MXTLbY5ie2ViQYPzZZk> Mn\OOUDPxE1? M3e5W|UhcA>? NIXrOGNFVVOR NWrpVpZFW3WycILld5NmeyC2aHWgd4lodmGuaX7nJI9nKEGtdDDhcoQhTXKt MXWyNFU4OTB4OR?=
HepG2 NUnNbphQS3m2b4TvfIlkKEG|c3H5 MlL5NVAh|ryP NFX6SoMzPCCq MVvEUXNQ MkPyV4Vve2m2aYrld{Bz\XOrc4ThcpQh[2WubIOgeI8hfGinIHP5eI91d3irYzDl[oZm[3Rib3[gd49z[W[nbnni MUKyNVIxPTl{NR?=
Sk-Hep1 NUfNc4h6S3m2b4TvfIlkKEG|c3H5 M2DKdFExKM7:TR?= NFjY[ZQzPCCq NX6zcVBPTE2VTx?= M1fpbHNmdnOrdHn6[ZMhemW|aYP0ZY51KGOnbHzzJJRwKHSqZTDjfZRwfG:6aXOg[YZn\WO2IH;mJJNwemGoZX7pZi=> NYqySodrOjF{MEW5NlU>
OCUT1 cells harbored PIK3CA (H1047R+/+) M4nReGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7iU5ZLOyEQvF2= NX3oT3gyPSCm NEGwPYFFVVOR MV7JR|UxRTBwMUSg{txO MVqyNVI5QTJ4Nx?=
K1 cells harbored PIK3CA (E542K+/+) NGXvPXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jX[lMh|ryP MmWwOUBl MXnEUXNQ Mlj5TWM2OD1yLkWyJO69VQ>? NXzUOmJROjF{OEmyOlc>
FTC133 cells harbored PTEN (allele deletion and R130+) NGj0O|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHyVJQ{KM7:TR?= NEjMNow2KGR? NHjxcFlFVVOR MXHJR|UxRTBwMUig{txO Ml3LNlEzQDl{Nke=
C643 cells harbored HRAS (G13R+/−) NHm3UXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jYfFMh|ryP NIj0cJQ2KGR? MnnOSG1UVw>? NVr2RXVnUUN3ME2wMlI4KM7:TR?= NY\t[2hIOjF{OEmyOlc>
Hth7 cells harbored NRAS (Q61R+/−) NILFfotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzjN{DPxE1? MUC1JIQ> NXzlbpZwTE2VTx?= MkLoTWM2OD12LkWg{txO MUeyNVI5QTJ4Nx?=
TPC1 cells harbored RET/PTC1 rearrangement NYH0PWZ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVyzJO69VQ>? NWqzPVBVPSCm NH\oXWxFVVOR NYLlZ4JvUUN3ME2wMlU6KM7:TR?= NYK4cINKOjF{OEmyOlc>
Hth74 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2TFdVMh|ryP MWG1JIQ> MYTEUXNQ M3HOSmlEPTB;Mj6xPUDPxE1? M2jORVIyOjh7Mk[3
KAT18 MnfXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvGb41WOyEQvF2= MWe1JIQ> MVfEUXNQ NF7CVWtKSzVyPUSuOlIh|ryP NF\0d3YzOTJ6OUK2Oy=>
SW1736 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoThNVAxKM7:TR?= MW[1JIQ> NIDQTFZFVVOR NITyNYNKSzVyPUS3MlU3KM7:TR?= Mm\xNlEzQDl{Nke=
WRO MofTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;kWnRLOTByMDFOwG0> NGrEbok2KGR? NWGwXmtUTE2VTx?= M3fkPWlEPTB-MUCwNEDPxE1? MlfLNlEzQDl{Nke=
TAD2 Ml7kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;Y[ncyODByIN88US=> MlLaOUBl MXXEUXNQ MVfJR|UxRjFyMECg{txO MXSyNVI5QTJ4Nx?=
LN229 MUPBdI9xfG:|aYOgRZN{[Xl? NF:zXVcxNjVizszN MlLWOlAhcA>? MYLEUXNQ NUP4RmE2SXWpbXXueJMh[XCxcITv[4VvcWNiZX\m[YN1eyCxZjDn[YZqfGmwaXK= MnjQNlIxPTd7MUS=
T98G MlvGRZBweHSxc3nzJGF{e2G7 MmLiNE42KM7:TR?= NUjhWGg5PjBiaB?= MmLmSG1UVw>? NFnCOo5CfWevZX70d{BieG:ydH;n[Y5q[yCnZn\lZ5R{KG:oIHfl[ol1cW6rYh?= NF\OTnQzOjB3N{mxOC=>
HC11 M{XBfWZ2dmO2aX;uJGF{e2G7 NYXvfYpoOTBizszN M2TxS|I1KGh? MXrEUXNQ NEDB[lNKdmirYnn0d{DPui2lYYPlbY4h[W6mIFHEVnAhe3mwdHjld4l{ MWqyNlQzPjZ{MR?=
MOLT-4 Ml;xR5l1d3SxeHnjJGF{e2G7 NEfvZVIyOCEQvF2= M4XJeVQ5KGh? M1LMe2ROW09? NGL4ZmhKSzVyPUGuO-KBkc7:TR?= NYrFTVRXOjJ4MUSyOFM>
CEM-R MVHDfZRwfG:6aXOgRZN{[Xl? NGmxdm8yOCEQvF2= NE\VN401QCCq M3rCO2ROW09? M1T2dmlEPTB;Mz6z5qCK|ryP NXizO4NiOjJ4MUSyOFM>
CEM-S MWjDfZRwfG:6aXOgRZN{[Xl? MlS1NVAh|ryP NIf6TYw1QCCq M{DJV2ROW09? MYXJR|UxRTVwMfMAje69VQ>? NWnNNmxsOjJ4MUSyOFM>
MOLT-4 NV2xU5BjTnWwY4Tpc44hSXO|YYm= MlPxNVAh|ryP M2PsOFI1KGh? NIPITpVFVVOR M3PnN2Jtd2OtczDj[YxteyCrbjD0bIUhTzBxR{GgdIhie2Vib3[geIhmKGOnbHygZ5lkdGV? NWTBUW05OjJ4MUSyOFM>
MOLT-4 Mk\rSpVv[3Srb36gRZN{[Xl? Mn3aOQKBkc7:TR?= MmDWOEBp NWq3dmRPTE2VTx?= NYjjN5k4UW6lcnXhd4V{KHSqZTDhcY92dnRib3[gZ4xm[X[nZDDMR|NCN0JuIHGge4VtdC2nc4ThZoxqe2inZDDheZRweGijZ4mgcYFzc2W{ MX2yNlYyPDJ2Mx?=
CEM-R MWTGeY5kfGmxbjDBd5NigQ>? NEnVWWg16oDLzszN MV[0JIg> NIXuXGpFVVOR NX22S4RJUW6lcnXhd4V{KHSqZTDhcY92dnRib3[gZ4xm[X[nZDDMR|RCN0JuIHGge4VtdC2nc4ThZoxqe2inZDDheZRweGijZ4mgcYFzc2W{ MWWyNlYyPDJ2Mx?=
CEM-S NYfGWo1MTnWwY4Tpc44hSXO|YYm= M2m5O|TjiIoQvF2= M4jlR|QhcA>? NX2wdYhuTE2VTx?= NFfW[VJKdmO{ZXHz[ZMhfGinIHHtc5VvfCCxZjDjcIVifmWmIFzDOWEwSixiYTD3[YxtNWW|dHHicIl{cGWmIHH1eI9xcGGpeTDtZZJs\XJ? MYqyNlYyPDJ2Mx?=
HepG2 cell MYHLbY5ie2ViQYPzZZk> NEDtbHIzOCEQvF2= NWP3R3BjOjRiaB?= MYjEUXNQ MmHHSI94dnKnZ4XsZZRmeyC2aHWgdIhwe3Cqb4L5cIF1cW:wIH;mJGFMXA>? NHXrRoEzOzd7N{OxPS=>
HepG2 cell NVPrSZRvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmf1N|Ah|ryP M1vmTVI1KGh? MVPEUXNQ MnvqTY5pcWKrdIOgZ4VtdCCpcn;3eIg> MUGyN|c6PzNzOR?=
HepG2 cell MmTSRZBweHSxc3nzJGF{e2G7 NX;ofFIyOjBizszN MoC1NlQhcA>? MUPEUXNQ NEnEb5pKdmS3Y3XzJINmdGxiYYDvdJRwe2m| MkXxNlM4QTd|MUm=
GEO Mke5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrlT5c2ODBibl2= MWi3NkBp NEWwOFlFVVOR MV;Jcohq[mm2czDj[YxtKGe{b4f0bC=> M{nMSFI1PThzMkOx
CNE-1 NHz6[nZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWOxNEDPxE1? MXi5OkBp MorBSG1UVw>? NWm5PZVWUUN3ME2yMlk3KM7:TR?= M3P1PVI2OzN4OUK1
CNE-2 NF3K[ZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mkm0NVAh|ryP M{nrPFk3KGh? MnfNSG1UVw>? Mk\qTWM2OD12LkWzJO69VQ>? M2rrTlI2OzN4OUK1
HONE-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXxXnIyOCEQvF2= MVK5OkBp M13kfGROW09? NX3CVYdKUUN3ME2zMlM4KM7:TR?= MYGyOVM{Pjl{NR?=
SUNE-1 NEW2OJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nCflExKM7:TR?= M3iwNlk3KGh? MYDEUXNQ NVTLW2RRUUN3ME2wMlUzKM7:TR?= MnP4NlU{OzZ7MkW=
CNE-2 MXjGeY5kfGmxbjDBd5NigQ>? NUTpOmpSOTBizszN NVLxR4RmPDhiaB?= MlXWSG1UVw>? MWjJcoR2[2W|IHPlcIwh[3mlbHWgZZJz\XO2IHH0JGcy MmXDNlU{OzZ7MkW=
HONE-1 NFe4UndHfW6ldHnvckBCe3OjeR?= Mk\MNVAh|ryP M{fJdVQ5KGh? NHTJSHNFVVOR MWjJcoR2[2W|IHPlcIwh[3mlbHWgZZJz\XO2IHH0JGcy M{\UbVI2OzN4OUK1
NEC8 MmDzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPP[YRNUUN3ME2wMlA6PjVzIN88US=> NYPXRVNLW0GQR1XS
P12-ICHIKAWA Mnm2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDKTWM2OD1yLkGxOlIh|ryP NEjjWlBUSU6JRWK=
MDA-MB-175-VII MlLaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGrsNWRKSzVyPUCuNVM4OzhizszN MWrTRW5ITVJ?
AsPC-1 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTBwMkKxNlIh|ryP MmS2V2FPT0WU
T47D M{e1fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTBwMkiyOUDPxE1? NF;uNYpUSU6JRWK=
HH NXfrN4FNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXu[mNKSzVyPUCuN|AzQDNizszN NHLobYdUSU6JRWK=
MOLT-16 NEHR[JZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTBwM{CzNkDPxE1? MkT2V2FPT0WU
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HOP-92 MnXpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVq4fYIzUUN3ME2wMlg4OjJ|IN88US=> MmXjV2FPT0WU
OAW-42 NVfr[YxzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnlT5FKSzVyPUCuPFg4OiEQvF2= MXfTRW5ITVJ?
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SK-NEP-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjlXm5JUUN3ME2xMlE1PDh3IN88US=> M1nTUXNCVkeHUh?=
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LoVo M1Pld2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjYVHZKSzVyPUGuN|I2OzRizszN NXyxS5FDW0GQR1XS
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MFM-223 MnL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jld2lEPTB;MT6zOFQ3OSEQvF2= MV3TRW5ITVJ?
PA-1 M3LPU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\DZmlEPTB;MT6zOVI3PSEQvF2= M2i4PHNCVkeHUh?=
697 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jGWGlEPTB;MT6zO|YyPiEQvF2= M4rYR3NCVkeHUh?=
QIMR-WIL M1\pS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVG5co4yUUN3ME2xMlQ6OTF4IN88US=> MV;TRW5ITVJ?
HOS MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLCXGlKSzVyPUGuOFk2PThizszN NH7Y[5dUSU6JRWK=
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ME-180 MkPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGj4S2dKSzVyPUGuOVY5QTFizszN MkTHV2FPT0WU
HCC2218 NWSzdIVWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTFwNkiyNlUh|ryP MVXTRW5ITVJ?
CAL-54 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nCRWlEPTB;MT63NVI1OiEQvF2= M3rodHNCVkeHUh?=
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BV-173 NXfZNWxJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnUUZlrUUN3ME2xMlgyODd2IN88US=> M1;LR3NCVkeHUh?=
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SW1088 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrVPZk1UUN3ME2xMlk1PjB4IN88US=> MX3TRW5ITVJ?
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NCI-H1395 M2XwSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHMTWM2OD1{LkG4NFkyKM7:TR?= NHfBUYJUSU6JRWK=
GAMG MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUi5NllKUUN3ME2yMlI{QDR3IN88US=> M13Zc3NCVkeHUh?=
LB1047-RCC MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{T4XWlEPTB;Mj6yOFMyPyEQvF2= MmS3V2FPT0WU
MN-60 NYnweXN[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PsVmlEPTB;Mj6yPVkzOyEQvF2= M131NXNCVkeHUh?=
OAW-28 NUPWWllbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7VVFMxUUN3ME2yMlI6QTVzIN88US=> MWHTRW5ITVJ?
NCI-H2228 M33JW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{DMd2lEPTB;Mj6zNVU2OiEQvF2= MVXTRW5ITVJ?
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NB69 MkKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTJwM{i5PFMh|ryP MWfTRW5ITVJ?
VM-CUB-1 M3vkWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnlSHZKSzVyPUKuN|kxQDNizszN MU\TRW5ITVJ?
D-423MG MmjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;ZUFhKSzVyPUKuOFExPDRizszN Mn3sV2FPT0WU
EW-18 NYrWbGhLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTJwNEG5N|kh|ryP M1njcnNCVkeHUh?=
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T-24 M3\uVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWqzRopEUUN3ME2yMlQ4QDhzIN88US=> NGf3dGxUSU6JRWK=
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ES3 NWHwPI85T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7PdVROUUN3ME2yMlQ6PzV7IN88US=> MXfTRW5ITVJ?
RXF393 NGO2SFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnuzTWM2OD1{Lk[wOFg4KM7:TR?= MnXyV2FPT0WU
RPMI-8226 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTJwNkK5OVMh|ryP MoPEV2FPT0WU
AGS M17SV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTJwN{KxN|ch|ryP NIPKZ41USU6JRWK=
HCC1395 NUHD[YJ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXv1SpJXUUN3ME2yMlc2OTh5IN88US=> MlLiV2FPT0WU
MV-4-11 NEnrS|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTJwN{WyOlYh|ryP NFrsdZpUSU6JRWK=
A204 NVj4OnVqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTJwOEO4O|Ih|ryP MmXPV2FPT0WU
MCF7 MkmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTJwOE[xNVch|ryP NWHlflJPW0GQR1XS
SNU-423 M1OzPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFX1SIJKSzVyPUKuPFkzPDJizszN MVrTRW5ITVJ?
NCI-H1048 NWPHT4dET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTJwOU[4OlUh|ryP NXXGW3A1W0GQR1XS
GR-ST NHrlNIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nhcWlEPTB;Mz6wOFYyOSEQvF2= NYjQSWhDW0GQR1XS
EoL-1- MlL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTNwMEewOVgh|ryP M2e1T3NCVkeHUh?=
HuH-7 NWexOolPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkW4TWM2OD1|LkC5OFY1KM7:TR?= MYnTRW5ITVJ?
OS-RC-2 NVTsOIFwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrqc5ZpUUN3ME2zMlEyOTlizszN NH7yXYhUSU6JRWK=
EW-3 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\hTWM2OD1|LkG5OVI6KM7:TR?= M1jpRXNCVkeHUh?=
NCI-H747 NGm1NXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2S5bGlEPTB;Mz6yNFY6PCEQvF2= NGG3UplUSU6JRWK=
EW-16 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTNwMkG4O|kh|ryP M2GzXnNCVkeHUh?=
DOK MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrHVlZKSzVyPUOuNlI5PTlizszN NWrqVoJwW0GQR1XS
HCC2157 NFezcpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTNwM{ixO|kh|ryP NF3HdGhUSU6JRWK=
OVCAR-3 NUXmUVRjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPpfVVKSzVyPUOuOFA4QDZizszN M1rXOnNCVkeHUh?=
NCI-H1623 Ml\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXFTWM2OD1|LkSxNlI1KM7:TR?= NY\6RVN1W0GQR1XS
H4 M2njU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTNwNEW2NlYh|ryP M3zFO3NCVkeHUh?=
SW1710 NVXTVoxvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jQemlEPTB;Mz60OlY4QCEQvF2= Mn[5V2FPT0WU
RT-112 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTNwNUKzPFgh|ryP MUfTRW5ITVJ?
DMS-114 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjuWmFKSzVyPUOuOlIzPzhizszN M1i2ZnNCVkeHUh?=
AN3-CA MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PrT2lEPTB;Mz62NlQ2PiEQvF2= NFzmXXJUSU6JRWK=
KNS-62 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUXiNXJ5UUN3ME2zMlY{OzN6IN88US=> M2jEfHNCVkeHUh?=
SJRH30 MnL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTNwNkmxNlIh|ryP Ml6wV2FPT0WU
G-402 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nHTWlEPTB;Mz63NFcyOSEQvF2= M{PFc3NCVkeHUh?=
MHH-PREB-1 NY\HRmllT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4D6SGlEPTB;Mz63NlA{QCEQvF2= MYTTRW5ITVJ?
P30-OHK NVm1eoJ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXT5PXhpUUN3ME2zMlgxQTd4IN88US=> Mm\KV2FPT0WU
RVH-421 M3fuZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGezdFdKSzVyPUOuPFE4QDhizszN MYTTRW5ITVJ?
LU-134-A M3zw[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWj4bmNFUUN3ME2zMlg5PDJ6IN88US=> MljJV2FPT0WU
ECC10 Mlq0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTyVldkUUN3ME2zMlk{PjJ{IN88US=> NWnET3k{W0GQR1XS
TGW NGnvVGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTlW|NkUUN3ME20MlAzOzB3IN88US=> MWXTRW5ITVJ?
MLMA NXT6OWM5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljXTWM2OD12LkCyPVY3KM7:TR?= MoTQV2FPT0WU
SCC-25 M4LhN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TBUmlEPTB;ND6wOlU3PiEQvF2= MlrKV2FPT0WU
TYK-nu M174VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTRwMEm1N|Qh|ryP NFTsSJdUSU6JRWK=
LAMA-84 NVrQb2V2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnTb3NuUUN3ME20MlE1OTlzIN88US=> M1nQXXNCVkeHUh?=
Calu-3 NHvRZ2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTRwMkS0NVYh|ryP MWHTRW5ITVJ?
NCI-H460 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvyOZhKSzVyPUSuNlY1PDNizszN MVnTRW5ITVJ?
EGI-1 Mlm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXpcGNbUUN3ME20MlM4Pzd6IN88US=> NEGxRolUSU6JRWK=
NCI-H292 Mn3PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTRwM{ixOFYh|ryP NIjKcY1USU6JRWK=
HCE-T MkLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\x[mlEPTB;ND60NVU4QSEQvF2= MUnTRW5ITVJ?
EW-11 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTRwNEG4N|gh|ryP MnvpV2FPT0WU
ATN-1 NGjFWXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvQfphKSzVyPUSuOFQ{ODRizszN MWHTRW5ITVJ?
NB5 NUDENm8yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13nbWlEPTB;ND61N|Y6PyEQvF2= MYDTRW5ITVJ?
KLE MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3vTWM2OD12LkewNVk5KM7:TR?= M1nIbHNCVkeHUh?=
CAL-39 MmDZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWO5Z4ZuUUN3ME20MlczOTR4IN88US=> M1P3NXNCVkeHUh?=
TI-73 Mli3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHqflZKSzVyPUSuPFA3ODlizszN MUnTRW5ITVJ?
HO-1-N-1 NF;YbZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmG0TWM2OD12Lkm0NkDPxE1? NV\r[pp1W0GQR1XS
786-0 NFnpc2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTRwOUS2O|Mh|ryP NFvzdWFUSU6JRWK=
SK-N-DZ Mn7ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrVOVdRUUN3ME20Mlk3OTR{IN88US=> MoPMV2FPT0WU
NCI-H446 MlKwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTVwMkCwNFkh|ryP Ml3DV2FPT0WU
ETK-1 NGLkRpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfXTWM2OD13LkKxNVY2KM7:TR?= NGCwepBUSU6JRWK=
BT-20 NVXOUpRpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4i4PGlEPTB;NT6yNVM2OyEQvF2= MkniV2FPT0WU
MEL-HO M{X1[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVWwPIlmUUN3ME21MlM4OzN4IN88US=> MlvRV2FPT0WU
CAL-27 MkD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDYWHFtUUN3ME21MlQ3OzN7IN88US=> M2W0OHNCVkeHUh?=
SW872 M{TnWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TlOmlEPTB;NT61PVQzQCEQvF2= NFPYWY1USU6JRWK=
RPMI-2650 Moj1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHvTWM2OD13Lk[2NVk6KM7:TR?= MYrTRW5ITVJ?
PFSK-1 NXnIO2V1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{T2dWlEPTB;NT63Nlc{OiEQvF2= NFzSWFRUSU6JRWK=
SF295 M2\keGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vYbWlEPTB;NT64NFY{OyEQvF2= M3[4UnNCVkeHUh?=
Becker Mnu3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrCTWM2OD13Lki2OFczKM7:TR?= Ml2zV2FPT0WU
Saos-2 NHnBZ4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHyTWM2OD13Lki2OVMh|ryP MnH6V2FPT0WU
SK-OV-3 NIG3SnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITkUY1KSzVyPUWuPVk5OTZizszN M3n2ZnNCVkeHUh?=
VMRC-RCZ M1n0OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTZwMEi3O|Mh|ryP M131NHNCVkeHUh?=
EW-22 M4rocmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHkU2ZKSzVyPU[uNVk3PDlizszN MlTyV2FPT0WU
BT-474 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlL1TWM2OD14LkKzN{DPxE1? MlHNV2FPT0WU
BFTC-909 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTZwM{CzOFUh|ryP NH\TVnZUSU6JRWK=
NB12 NFX4VZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTZwM{mwO|Eh|ryP MlXxV2FPT0WU
D-263MG NH75[FVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlm1TWM2OD14LkS1NVY6KM7:TR?= MUfTRW5ITVJ?
SNB75 NUfJR3h3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlj4TWM2OD14Lk[wNVQ{KM7:TR?= MWPTRW5ITVJ?
A704 NUT2TYxVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NID2N3hKSzVyPU[uOlMxPiEQvF2= MlPSV2FPT0WU
NCI-H1693 NWTWe4ZrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnITWM2OD14Lk[zOlA1KM7:TR?= M2r5fnNCVkeHUh?=
LN-405 M2\wSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTZwN{m2O|Ih|ryP M2XqV3NCVkeHUh?=
CHL-1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnWwTWM2OD14LkiwNFc6KM7:TR?= NVO1UodTW0GQR1XS
A498 NXr5e5ZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUTJR|UxRTZwOEG5OlEh|ryP M4jNeXNCVkeHUh?=
TE-12 M2exfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7pR2dKSzVyPU[uPFM5OTdizszN MV\TRW5ITVJ?
TE-6 M2\PWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;NTWM2OD14LkmzNFM5KM7:TR?= M3zPbHNCVkeHUh?=
AU565 NFrpeWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTZwOU[5OVch|ryP MknFV2FPT0WU
RD MnnZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1njOGlEPTB;Nj65PFI5PCEQvF2= NIrFU|FUSU6JRWK=
SW1463 NFH1O4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\pTWM2OD15LkGxNVY5KM7:TR?= NWHnblNYW0GQR1XS
LU-99A MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfKeFBjUUN3ME23MlE1OzJ{IN88US=> MlzZV2FPT0WU
NCI-H28 NIHaXFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTdwMkmyOEDPxE1? M{S4VHNCVkeHUh?=
MC-IXC NHzwSGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\YTWM2OD15LkS4OVc3KM7:TR?= NVjITGtEW0GQR1XS
GP5d M{T3bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXUTWM2OD15LkS4O|Y1KM7:TR?= NYqwe2tyW0GQR1XS
GB-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTdwNUS4NFQh|ryP MV;TRW5ITVJ?
CAL-33 NYjLN|RNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEi4PHBKSzVyPUeuOlYzOzNizszN MVfTRW5ITVJ?
MSTO-211H MlHwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDRToRKSzVyPUeuOlc{OzZizszN MlT0V2FPT0WU
TE-5 NEn2dZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEixVFFKSzVyPUeuO|k{OzRizszN NYP5TWxCW0GQR1XS
D-566MG NEDV[4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;KUWlEPTB;OD6wOFQzQSEQvF2= NX3YRY42W0GQR1XS
JVM-3 NELQUGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2ThV2lEPTB;OD6xOVI3QCEQvF2= MlGzV2FPT0WU
T98G NXrJSGk6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRThwMUiwOlch|ryP Mn72V2FPT0WU
HCC1954 M{nsT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEK3dHRKSzVyPUiuOFUyODRizszN M3Hkc3NCVkeHUh?=
SF126 MmjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRThwNEW5N|Yh|ryP NGD5XnNUSU6JRWK=
LB996-RCC M3LLOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkO0TWM2OD16LkWzNlU4KM7:TR?= NX3nN4NLW0GQR1XS
SKG-IIIa NIPHRmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PEe2lEPTB;OD62N|A3QSEQvF2= MYrTRW5ITVJ?
NCI-SNU-1 NH[yS2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13EXGlEPTB;OD62OFY1OyEQvF2= MlfBV2FPT0WU
LB771-HNC MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3UfldpUUN3ME24MlY1Pjl4IN88US=> MnPHV2FPT0WU
SCC-4 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRThwNkiyNVkh|ryP Mnj4V2FPT0WU
CAMA-1 NYrKcZQzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfUOXJKSzVyPUiuO|cyPDZizszN MmfOV2FPT0WU
D-502MG MoDOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTYT256UUN3ME24Mlc5PjJ7IN88US=> MX3TRW5ITVJ?
ESS-1 MmfxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRThwOEi3NFQh|ryP MXnTRW5ITVJ?
HEC-1 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRThwOEm4OlYh|ryP M2jvTnNCVkeHUh?=
NB10 M4TabWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTlwMEKyNlQh|ryP MnHDV2FPT0WU
8505C MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHCbmlKUUN3ME25MlA1OjN{IN88US=> MY\TRW5ITVJ?
EFO-27 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XGU2lEPTB;OT6xOlQyOiEQvF2= NWK1c4xyW0GQR1XS
HN M{\RTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2e5SmlEPTB;OT6xOlYzQCEQvF2= M2j2ZXNCVkeHUh?=
DSH1 M4CyU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfB[VhvUUN3ME25MlIxQDdizszN NYr2WnptW0GQR1XS
NBsusSR M2XKTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\wTWM2OD17LkK3OFAzKM7:TR?= NWnURlM{W0GQR1XS
LS-123 NIHEc4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDVemVKSzVyPUmuN|E4PjFizszN MWXTRW5ITVJ?
SHP-77 NEHO[2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXKTWM2OD17LkO5PVM2KM7:TR?= MXLTRW5ITVJ?
ACN MnriS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDYTWM2OD17LkWzNlc4KM7:TR?= M3XHcnNCVkeHUh?=
U251 NFjr[W1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTlwNkW1OFQh|ryP NWLrTI8{W0GQR1XS
A431 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTlwOECyN|gh|ryP MXvTRW5ITVJ?
5637 NIK4T|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTlwOES5PFQh|ryP MmLUV2FPT0WU
MDA-MB-157 MnzGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTlwOUK4O|gh|ryP NVLEXo5KW0GQR1XS
A101D M{nh[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTlwOUm5O|Qh|ryP NEDJNVBUSU6JRWK=
YKG-1 NGCyUnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DhXmlEPTB;MUCuNlAxPiEQvF2= MVzTRW5ITVJ?
LAN-6 MlKwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn31TWM2OD1zMD6yNVY1KM7:TR?= NUDjeXJOW0GQR1XS
OVCAR-5 NVjMOItbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHWUoFKSzVyPUGwMlI1OzNizszN M2DWbHNCVkeHUh?=
A549 NUfHSGFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF23PJJKSzVyPUGwMlM6PzNizszN M4HqOHNCVkeHUh?=
no-11 NHfHNVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPXTWM2OD1zMD60N|U{KM7:TR?= NVXa[|NbW0GQR1XS
SF539 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{X0bWlEPTB;MUCuPVA1OSEQvF2= M2nFPHNCVkeHUh?=
A388 NXzYN2d5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUT3RYUyUUN3ME2xNU4{QDl5IN88US=> NFvybFVUSU6JRWK=
DEL MkHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\lTWM2OD1zMT60NlQh|ryP NWjlXoZTW0GQR1XS
SW954 M1vZ[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIm4cFNKSzVyPUGxMlQ3PjhizszN Mn\ZV2FPT0WU
TK10 NUTnN5pYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPzTWM2OD1zMT61NlcyKM7:TR?= NV;C[oZIW0GQR1XS
SW756 M{H1SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\W[mlEPTB;MUGuOVI6PCEQvF2= MXzTRW5ITVJ?
PC-3 M1e2d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFXnSWVKSzVyPUGxMlU4PjRizszN NWjSepVKW0GQR1XS
ONS-76 NHrXPJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTFzLk[zOkDPxE1? NYPDZ4s4W0GQR1XS
A427 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjzTWM2OD1zMT63NFk{KM7:TR?= MWjTRW5ITVJ?
MEG-01 Mm\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;6TWM2OD1zMT63OVA6KM7:TR?= NVXnUoFTW0GQR1XS
BB30-HNC NYfLb4dvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nMcmlEPTB;MUGuO|k5OiEQvF2= M37hOHNCVkeHUh?=
NCI-H1299 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1jDWWlEPTB;MUGuPFA6OyEQvF2= MlnRV2FPT0WU
GCT NWHFZnpuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnkTWM2OD1zMT64NlI5KM7:TR?= NVfifmlMW0GQR1XS
D-247MG NIruVpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3m0ZWlEPTB;MUGuPVY3OyEQvF2= NGX2VI1USU6JRWK=
CFPAC-1 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTFzLkm3PFIh|ryP Mn:xV2FPT0WU
EKVX MoLPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTF{LkCzNVMh|ryP NFPPNFJUSU6JRWK=
CAL-51 NWXaeXVoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPVTWM2OD1zMj6wO|E3KM7:TR?= MXrTRW5ITVJ?
BB49-HNC NE\JO5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33YVGlEPTB;MUKuNVE4PyEQvF2= M1zpSXNCVkeHUh?=
RPMI-7951 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljTTWM2OD1zMj6xPFU1KM7:TR?= M4H6eHNCVkeHUh?=
RH-1 NIPXR25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYi5b2UyUUN3ME2xNk4zOTh2IN88US=> M1u4VXNCVkeHUh?=
BCPAP M2m4RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PzZ2lEPTB;MUKuOFc1QSEQvF2= MVLTRW5ITVJ?
GCIY MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHzTWM2OD1zMj61NlA6KM7:TR?= MojPV2FPT0WU
KNS-81-FD NEPYeJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTue4F3UUN3ME2xNk42QDZ7IN88US=> MVfTRW5ITVJ?
KYSE-140 MmTmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXMTWM2OD1zMj64OVk2KM7:TR?= NIfHbXJUSU6JRWK=
Ca-Ski MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGqxZ4xKSzVyPUGyMlkxPDFizszN M{K1cHNCVkeHUh?=
TGBC1TKB MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFrYZ4xKSzVyPUGyMlkyOTVizszN MUjTRW5ITVJ?
HCC1187 M3OxRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHix[YpKSzVyPUGzMlE6OTJizszN MVnTRW5ITVJ?
SJSA-1 MlfxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHJTWM2OD1zMz6yN|I4KM7:TR?= MXzTRW5ITVJ?
CTV-1 NXX1Vo9NT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfTbWtKSzVyPUGzMlM1PSEQvF2= M3HMeHNCVkeHUh?=
WM-115 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXQeYZKSzVyPUGzMlY1QDNizszN MnHOV2FPT0WU
CHP-212 M3zibmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LtSGlEPTB;MUOuPVc{QSEQvF2= MUDTRW5ITVJ?
SCC-15 NEnUU3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjVTWM2OD1zMz65O|c2KM7:TR?= MkHSV2FPT0WU
BPH-1 MnntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHmTWM2OD1zND6xOlY1KM7:TR?= NVy2dG5rW0GQR1XS
SW780 NGXvSZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTF2LkWwNlUh|ryP NFvlZlVUSU6JRWK=
NCI-H2291 M4HyZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fIXGlEPTB;MUSuOVg4QCEQvF2= NVvLOmZ{W0GQR1XS
JEG-3 MojkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXKTWM2OD1zND62N|I3KM7:TR?= NITQc|VUSU6JRWK=
CAL-120 MlvJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XCOGlEPTB;MUSuO|AzPyEQvF2= NWSxNIRvW0GQR1XS
NCI-H23 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTF2Lke5PVch|ryP MXjTRW5ITVJ?
MS-1 M1PLWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV:2XVhPUUN3ME2xOE46PjFzIN88US=> M{PhVHNCVkeHUh?=
PC-14 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHFTWM2OD1zND65OlU1KM7:TR?= M{HGSHNCVkeHUh?=
D-283MED NX;TPZhVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\VRmlEPTB;MUWuNFEyOSEQvF2= NVLi[2oyW0GQR1XS
OE19 NIO0flVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7TbmFKSzVyPUG1MlE2PDFizszN NEnpOmlUSU6JRWK=
CAS-1 NVfNOZdZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmq4TWM2OD1zNT60NVg1KM7:TR?= MVHTRW5ITVJ?
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NCI-H2342 MlXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnBNGdGUUN3ME2yOU4yQTV|IN88US=> MUXTRW5ITVJ?
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CCRF-CEM M1H6d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTJ2Mz63PVUh|ryP MUPTRW5ITVJ?
SH-4 Ml:wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHLR25KSzVyPUK0Ok4xQSEQvF2= NFzXc2NUSU6JRWK=
LS-1034 NH;ZZmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LabGlEPTB;MkS2MlI3PiEQvF2= MUjTRW5ITVJ?
NCI-H2347 NH7J[FhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTJ2Nz63NVMh|ryP MX\TRW5ITVJ?
RPMI-8866 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnv2TWM2OD1{NEmuNlch|ryP M1K5N3NCVkeHUh?=
GAK MkXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXnUWZxUUN3ME2yOVMvODB{IN88US=> MVvTRW5ITVJ?
NB6 M4\OWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLxTWM2OD1{N{CuNUDPxE1? NILZUVNUSU6JRWK=
COLO-680N NV3wcVM3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\X[ppKSzVyPUK3Nk42OjdizszN NHz2bXFUSU6JRWK=
RERF-LC-MS NW\zSZFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3r5TmlEPTB;Mke2MlAxPyEQvF2= MnXmV2FPT0WU
TGBC11TKB NES5VVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWq5XopLUUN3ME2yO|gvOTd6IN88US=> M{HSRnNCVkeHUh?=
C8166 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7UTYwxUUN3ME2yO|gvPTB4IN88US=> NWHPb3lNW0GQR1XS
HDLM-2 NGDFbZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTJ7ND60NFkh|ryP M{XrNnNCVkeHUh?=
IGR-1 MkHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1PIemlEPTB;Mkm1MlY2QSEQvF2= M4DDd3NCVkeHUh?=
FADU MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjtTWM2OD1{OUeuOVEh|ryP MXvTRW5ITVJ?
L-428 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zYXmlEPTB;Mkm3MlYyPiEQvF2= Mm\IV2FPT0WU
LU-65 NFy2[XJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TvdmlEPTB;M{C0MlMzKM7:TR?= NHfFdYtUSU6JRWK=
HEL NX6xcG5qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLsVnhJUUN3ME2zNFkvQTh|IN88US=> MlX4V2FPT0WU
NCI-H810 NIjy[3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NES3[WFKSzVyPUOxNE42PyEQvF2= MUTTRW5ITVJ?
C3A MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHFTWM2OD1|MUGuPFAzKM7:TR?= MlLOV2FPT0WU
NCI-H630 M3;4dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPjTWM2OD1|M{KuNlk1KM7:TR?= MoPzV2FPT0WU
KP-N-YN MmDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjNVpZwUUN3ME2zOFEvOTJ|IN88US=> NHPJXIVUSU6JRWK=
MOLT-13 MlX5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\UTWM2OD1|NEKuN|I3KM7:TR?= MUPTRW5ITVJ?
NCI-H1993 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXztfJZbUUN3ME2zOFIvOzZ3IN88US=> M331fHNCVkeHUh?=
BE-13 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml70TWM2OD1|NESuNVY4KM7:TR?= MXXTRW5ITVJ?
IST-SL1 NWm5cpJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Moi0TWM2OD1|NEeuOFAyKM7:TR?= NEXRbY9USU6JRWK=
TE-9 NVXJ[YZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTN4Mz61PFkh|ryP M2LuTnNCVkeHUh?=
LU-135 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3faV2lEPTB;M{[3MlA{PSEQvF2= MkHYV2FPT0WU
T84 NVX3T40zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jheGlEPTB;M{e0MlcyOiEQvF2= NYHReXFlW0GQR1XS
K-562 NX;jeXA5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PWd2lEPTB;M{izMlM3KM7:TR?= MX3TRW5ITVJ?
SBC-5 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjZOotiUUN3ME2zPFYvQTh3IN88US=> MlLOV2FPT0WU
NB17 M4\RS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPxdo9UUUN3ME2zPVIvPTl4IN88US=> NIfKWJNUSU6JRWK=
NCI-H2052 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTN7OD60O|Ih|ryP MkXNV2FPT0WU
HCC38 Ml65S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1yyVGlEPTB;NECxMlU6OyEQvF2= MWHTRW5ITVJ?
NCI-H69 Ml\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTR2MT6wPFMh|ryP M1y3NXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT(T308) / p-AKT(S473) / pGSK3β(S9) / pFOXO1 / pPRAS(T246) / pBAD / pS6K(T389) / p4E-BP1; 

PubMed: 22932669     


ZR75-1 cells were treated with MK-2206 150 nmol/L and collected after indicated hours. Akt signaling was assessed by Western blotting. 

22932669
Growth inhibition assay
Cell viability; 

PubMed: 24665203     


(A) MK-2206 reduced the viability of anaplastic thyroid carcinoma (ATC) cells. CAL62 and KAT4 cells were treated with MK-2206 for 72 hours, followed by measurement with a cell viability assay. (B) In vitro cell viability assay of combination with MK-2206 and tyrphostin AG 1296 in KAT4 cells. 

24665203
Immunofluorescence
LC3; 

PubMed: 24490764     


Immunofluorescence staining of LC3A (green) and DAPI (blue) in M368 cells following treatment with either MK-2206, Carboplatin and Paclitaxel or the combination of all three agents for 72h.

CFTR/LC3; 

PubMed: 28794469     


Co-localisation of CFTR (red- 549nm) and LC3 (green- 488nm) in ΔF508 CFBE41o- cells treated with MK-2206 and a negative control CFBE41o- cells at 37 ℃. Nuclei were stained with DAPI (blue- 358nm). Scale bar is 10 µm.

HMGA2; 

PubMed: 24476133     


Immunofluorescent staining for HMGA2 of leiomyoma cells in control and MK-2206-treated cells was done. Cells were costained with DAPI. Immunointensity of HMGA2 expression was measured and calculated. *, P < .05; **, P < .01; ***, P < .001.

24490764 28794469 24476133
ELISA
VEGFA; 

PubMed: 29449553     


VEGF-A protein expression of conditioned medium was determined by ELISA in cervical cancer cells. The results were analyzed using one-way ANOVA. Column, mean (n = 3); bars, SD. **P < 0.01, compared with control. ##P < 0.01, compared with NF90.

29449553
In vivo MK-2206 shows 60% TGI and inhibits more than 70 % of phospho-Akt1/2 (T308 and S473) in A2780 ovarian cancer xenografts at a dose of 240 mg/kg. [1] MK-2206 exhibits significant antitumor activity in NCI-H292 xenograft in combination with erlotinib or lapatinib. [2]

Protocol

Kinase Assay:

[4]

+ Expand

Akt kinases assay:

Akt kinases are assayed by a GSK-derived biotinylated peptide substrate. The extent of peptide phosphorylation is determined by Homogeneous Time Resolved Fluorescence (HTRF) using a lanthanide chelate (Lance)-coupled monoclonal antibody specific for the phosphopeptide in combination with a streptavidin-linked allophycocyanin (SA-APC) fluorophore which will bind to the biotin moiety on the peptide. When the Lance and APC are in proximity, a non-radiative energy transfer takes place from the Lance to the APC, followed by emission of light from APC at 655 nm. Working Solution: 100X protease inhibitor cocktail (PIC): 1mg/mL benzamidine, 0.5 mg/mL pepstatin, 0.5 mg/mL leupeptin, 0.5 mg/mL aprotinin; 10X assay buffer: 500 mM HEPES, pH7.5, 1% PEG, 16.6 mM EDTA, 1 mM EGTA, 1% BSA, 20 mM 9-glycerol phosphate; Quench buffer 50 mM HEPES pH 7.3, 16.6 mM EDTA, 0.1% BSA, 0.1% Triton X-100, 0.17 nM labeled monoclonal antibody, 0.0067 mg/mL SA-APC; ATP/MgCl2 working solution: 1X Assay buffer, 1 mM DTT, 1X PIC, 5% glycerol, active Akt; Peptide working solution: 1X Assay buffer, 1 mM DTT, 1X PIC, 5% glycerol, 2 TM GSK biotinylated peptide. The reaction is assembled by adding 16 µL of ATP/MgCl2 working solution to the appropriate wells. MK-2206 or vehicle (1.0 µL) is added followed by 10 µL of peptide working solution. The reaction is started by adding 13 μL of the enzyme working solution and mixing. The reaction is allowed to proceed for 50 min and then stopped by the addition of 60 µL HTRF quench buffer. The stopped reactions are incubated at room temperature for at least 30 min and then read in the instrument.
Cell Research:

[2]

+ Expand
  • Cell lines: A431, HCC827, NCI-H292, NCI-H358, NCI-H23, NCI-H1299, Calu-6 and NCI-H460 cells
  • Concentrations: 0, 0.3, 1 and 3 μM
  • Incubation Time: 72 or 96 hours
  • Method:

    MK-2206 is dissolved in DMSO as a stock solution and diluted by culture media before use. Cells are seeded at a density of 2-3 × 103 in 96-well plates and incubated for 24 hours. Then MK-2206 (0, 0.3, 1 and 3 μM) is added to the cells. Cell proliferation is determined after 72 or 96 hours.


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: SK-OV-3, NCI-H292, HCC70, PC-3, and NCI-H460 models in male CD1-nude mice
  • Formulation: Formulated in 30% Captisol
  • Dosages: 120 mg/kg
  • Administration: Orally administered
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL (29.14 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
15% Captisol
For best results, use promptly after mixing.
17 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 480.39
Formula

C25H21N5O.2HCl

CAS No. 1032350-13-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01802320 Active not recruiting Colon Mucinous Adenocarcinoma|Colon Signet Ring Cell Adenocarcinoma|Rectal Mucinous Adenocarcinoma|Rectal Signet Ring Cell Adenocarcinoma|Recurrent Colon Carcinoma|Recurrent Rectal Carcinoma|Stage IIIA Colon Cancer|Stage IIIA Rectal Cancer|Stage IIIB Colon Cancer|Stage IIIB Rectal Cancer|Stage IIIC Colon Cancer|Stage IIIC Rectal Cancer|Stage IVA Colon Cancer|Stage IVA Rectal Cancer|Stage IVB Colon Cancer|Stage IVB Rectal Cancer National Cancer Institute (NCI) March 2013 Phase 2
NCT01802320 Active not recruiting Colon Mucinous Adenocarcinoma|Colon Signet Ring Cell Adenocarcinoma|Rectal Mucinous Adenocarcinoma|Rectal Signet Ring Cell Adenocarcinoma|Recurrent Colon Carcinoma|Recurrent Rectal Carcinoma|Stage IIIA Colon Cancer|Stage IIIA Rectal Cancer|Stage IIIB Colon Cancer|Stage IIIB Rectal Cancer|Stage IIIC Colon Cancer|Stage IIIC Rectal Cancer|Stage IVA Colon Cancer|Stage IVA Rectal Cancer|Stage IVB Colon Cancer|Stage IVB Rectal Cancer National Cancer Institute (NCI) March 2013 Phase 2
NCT01859182 Withdrawn Adenocarcinoma of the Gallbladder|Adenocarcinoma With Squamous Metaplasia of the Gallbladder|Adult Primary Cholangiocellular Carcinoma|Advanced Adult Primary Liver Cancer|Cholangiocarcinoma of the Extrahepatic Bile Duct|Localized Unresectable Adult Primary Liver Cancer|Metastatic Extrahepatic Bile Duct Cancer|Recurrent Adult Primary Liver Cancer|Recurrent Extrahepatic Bile Duct Cancer|Stage II Gallbladder Cancer|Stage IIIA Gallbladder Cancer|Stage IIIB Gallbladder Cancer|Stage IVA Gallbladder Cancer|Stage IVB Gallbladder Cancer|Unresectable Extrahepatic Bile Duct Cancer National Cancer Institute (NCI) January 2013 Phase 2
NCT01783171 Completed Pancreatic Adenocarcinoma|Recurrent Pancreatic Carcinoma|Stage III Pancreatic Cancer AJCC v6 and v7|Stage IV Pancreatic Cancer AJCC v6 and v7|Unresectable Pancreatic Carcinoma National Cancer Institute (NCI) January 15 2013 Phase 1
NCT01776008 Terminated Estrogen Receptor Positive|HER2/Neu Negative|Recurrent Breast Carcinoma|Stage IIA Breast Cancer|Stage IIB Breast Cancer|Stage IIIA Breast Cancer|Stage IIIB Breast Cancer|Stage IIIC Breast Cancer National Cancer Institute (NCI) January 2013 Phase 2
NCT01859182 Withdrawn Adenocarcinoma of the Gallbladder|Adenocarcinoma With Squamous Metaplasia of the Gallbladder|Adult Primary Cholangiocellular Carcinoma|Advanced Adult Primary Liver Cancer|Cholangiocarcinoma of the Extrahepatic Bile Duct|Localized Unresectable Adult Primary Liver Cancer|Metastatic Extrahepatic Bile Duct Cancer|Recurrent Adult Primary Liver Cancer|Recurrent Extrahepatic Bile Duct Cancer|Stage II Gallbladder Cancer|Stage IIIA Gallbladder Cancer|Stage IIIB Gallbladder Cancer|Stage IVA Gallbladder Cancer|Stage IVB Gallbladder Cancer|Unresectable Extrahepatic Bile Duct Cancer National Cancer Institute (NCI) January 2013 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    What is your recommendation for preparing the solution and how would you deliver it to the mice (i.p. or gavage)?

  • Answer:

    You can resuspend MK-2206 in 15% Captisol at up to 17mg/ml. It's a suspension and is for oral gavage use only.

  • Question 2:

    I would want to know if MK-2206 could cross the blood brain barrier?

  • Answer:

    MK-2206 can cross BBB based on the following reference: https://clinicaltrials.gov/ct2/show/NCT01249105.

Akt Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID