MK-2206 2HCl

For research use only.

Catalog No.S1078

1364 publications

MK-2206 2HCl Chemical Structure

CAS No. 1032350-13-2

MK-2206 2HCl is a highly selective inhibitor of Akt1/2/3 with IC50 of 8 nM/12 nM/65 nM in cell-free assays, respectively; no inhibitory activities against 250 other protein kinases observed. MK-2206 2HCl induces autophagy and apoptosis in cancer cells. Phase 2.

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Selleck's MK-2206 2HCl has been cited by 1364 publications

Purity & Quality Control

Choose Selective Akt Inhibitors

Biological Activity

Description MK-2206 2HCl is a highly selective inhibitor of Akt1/2/3 with IC50 of 8 nM/12 nM/65 nM in cell-free assays, respectively; no inhibitory activities against 250 other protein kinases observed. MK-2206 2HCl induces autophagy and apoptosis in cancer cells. Phase 2.
Features The first allosteric small molecule inhibitor of Akt to enter clinical development.
Targets
Akt1 [1]
(Cell-free assay)
Akt2 [1]
(Cell-free assay)
Akt3 [1]
(Cell-free assay)
8 nM 12 nM 65 nM
In vitro

MK-2206 is an allosteric inhibitor and is activated by the pleckstrin homology domain. MK-2206 inhibits auto-phosphorylation of both Akt T308 and S473. MK-2206 also prevents Akt-mediated phosphorylation of downstream signaling molecules, including TSC2, PRAS40 and ribosomal S6 proteins. [1] MK-2206 inhibits Ras wild-type (WT) cell lines (A431, HCC827, and NCI-H292) more potently when compared to Ras-mutant cell lines (NCI-H358, NCI-H23, NCI-H1299, and Calu-6). MK-2206 also shows synergistic responses in combination with cytotoxic agents such as erlotinib or lapatinib in lung NCI-H460 or ovarian A2780 tumor cells. [2] MK-2206 or siRNA-mediated Akt inhibition strongly activates autophagy in human glioma cells. However, eukaryotic elongation factor-2 (eEF-2) silencing suppresses MK-2206-induced-autophagy, with a promotion of apoptotic cell death. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H69 NH7aOFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIS1S45KSzVyPUS0NU4xQDNizszN MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
HCC38 NFr2XIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTRyMT61PVMh|ryP M4DCVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
NCI-H2052 NHrkR3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmqxTWM2OD1|OUiuOFczKM7:TR?= NGTTUGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NB17 M1GydWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTN7Mj61PVYh|ryP NWTYVHlvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
SBC-5 NWfOR4FMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3HQWWlEPTB;M{i2Mlk5PSEQvF2= MkjxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
K-562 NYX1W2dnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LkUGlEPTB;M{izMlM3KM7:TR?= NInsXG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
T84 NWC2eIV7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTN5ND63NVIh|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
LU-135 NH31NZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVrPeZp6UUN3ME2zOlcvODN3IN88US=> Mk\IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
TE-9 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXyTWM2OD1|NkOuOVg6KM7:TR?= NI[2SW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
IST-SL1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\pPHhKSzVyPUO0O{41ODFizszN NFrmNW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
BE-13 NUH0dYJMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLiXFdHUUN3ME2zOFQvOTZ5IN88US=> M16xXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
NCI-H1993 NHHEVohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTN2Mj6zOlUh|ryP MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
MOLT-13 M1LkSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXixbIhxUUN3ME2zOFIvOzJ4IN88US=> MknXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NCI-H630 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkCzTWM2OD1|M{KuNlk1KM7:TR?= NF[2ZnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
KP-N-YN MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\ETWM2OD1|NEGuNVI{KM7:TR?= M13HU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
C3A M1rOdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnnZndlUUN3ME2zNVEvQDB{IN88US=> NGHoV2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
HEL MmD4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2D5e2lEPTB;M{C5Mlk5OyEQvF2= NVXv[4d[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
NCI-H810 NUC1bWVwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TSTGlEPTB;M{GwMlU4KM7:TR?= MlX4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
LU-65 NILI[HlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjSTWM2OD1|MESuN|Ih|ryP MnvLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
L-428 NYXHdY9nT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PqRWlEPTB;Mkm3MlYyPiEQvF2= NX7PZ45WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
FADU MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTJ7Nz61NUDPxE1? NVyxfHlGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
IGR-1 MmHzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjYcYRMUUN3ME2yPVUvPjV7IN88US=> NVHGSWFIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
HDLM-2 NF:wVGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU[wbZZuUUN3ME2yPVQvPDB7IN88US=> M1noUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
C8166 M4fpbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rEW2lEPTB;Mke4MlUxPiEQvF2= NVm0WW86RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
TGBC11TKB MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{[yXWlEPTB;Mke4MlE4QCEQvF2= NHuwVnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
RERF-LC-MS MmixS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7FRnZKSzVyPUK3Ok4xODdizszN MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
COLO-680N MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTJ5Mj61Nlch|ryP NEPjOmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NB6 NWXFbXJET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInzOXZKSzVyPUK3NE4yKM7:TR?= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
GAK NGLufHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlO4TWM2OD1{NUOuNFAzKM7:TR?= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
RPMI-8866 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\HTWM2OD1{NEmuNlch|ryP MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
NCI-H2347 M1T1[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzUPVJzUUN3ME2yOFcvPzF|IN88US=> MlHWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
LS-1034 MmOyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mkj5TWM2OD1{NE[uNlY3KM7:TR?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
SH-4 Ml3DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PuSmlEPTB;MkS2MlA6KM7:TR?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
CCRF-CEM MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEfaRoVKSzVyPUK0N{44QTVizszN MmP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
C2BBe1 M2racmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU[1NVFOUUN3ME2yN|EvQTB3IN88US=> NF[2RZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
COLO-320-HSR MlnyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrielQxUUN3ME2yNVYvOTJ3IN88US=> NF3VdGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
KNS-42 NFL2d3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXKybYdWUUN3ME2yNVYvPTd2IN88US=> Ml;ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
RCM-1 NGH6NJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfoOGJTUUN3ME2yNVQvPzZ{IN88US=> NVLqcIR2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
DBTRG-05MG Mom4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2H3VGlEPTB;MkC3MlkxQSEQvF2= M1X4fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SW982 NFnZSHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTJyNz65OFgh|ryP NV76eYNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
SW1116 MmezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrGTWM2OD1{MEeuNFc4KM7:TR?= M4rxR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
HuCCT1 M162N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXVTWM2OD1{MESuNlA5KM7:TR?= MnzLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NCI-H650 NVTqO5RKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\YV2VKSzVyPUKwNk4yODNizszN NFqx[Ik9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
COLO-678 NYnHZmNoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlfITWM2OD1{MEGuOFUh|ryP NUDxOopsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
HT-29 MmDZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULQeHNwUUN3ME2yNFEvOjF{IN88US=> M2nPOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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KYSE-150 NUX5VWVnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vuOGlEPTB;MUmxMlg5QCEQvF2= NUGzbpVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
OVCAR-4 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPVSndPUUN3ME2xPFgvOzN|IN88US=> MmLjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
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NCI-H82 NXPRNZBqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17mXGlEPTB;MUiwMlE3PSEQvF2= M3fpPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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no-10 MnHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVX3fWp[UUN3ME2xO|gvOTN2IN88US=> NV:2U2VbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
MDA-MB-231 NEDUcWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELDd3lKSzVyPUG3O{42ODNizszN M3;GTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
HCE-4 MlqwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;WZWRKSzVyPUG2Ok45PDVizszN Mn61QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NY MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;VTWM2OD1zNkWuN|E1KM7:TR?= NEPIe3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
A673 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTF4MT63NFUh|ryP NVnKe|U2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
SAS NGTsZ3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHnTWM2OD1zNkKuOlc5KM7:TR?= NHfLUXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
Capan-2 NXnBRVhJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2CyUWlEPTB;MU[wMlU2OyEQvF2= NUfwWm1bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
KU812 NFjMVodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTF3OD62O|Qh|ryP M1y2e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
NCI-H1304 MmjLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\hTWM2OD1zNUSuOlk1KM7:TR?= MnroQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
CAPAN-1 NIfDOVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mke4TWM2OD1zNUOuNFY1KM7:TR?= NWP3[lBSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
BEN Ml22S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnETWM2OD1zNUOuPVI5KM7:TR?= NE\R[ZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
KYSE-450 NHzrdmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TJcmlEPTB;MUWxMlU{QSEQvF2= M1jh[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
GT3TKB MnrLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjxOI1KSzVyPUG0PU46OjhizszN M3v6PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SK-MEL-3 NHzHN2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILnNodKSzVyPUG0O{45PzhizszN MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
OVCAR-8 NV7TNph3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfkTWM2OD1zNEWuOlM3KM7:TR?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
CP50-MEL-B M{S3R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fLdGlEPTB;MUS0MlUxOSEQvF2= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
COR-L88 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkLETWM2OD1zNEKuNVQh|ryP NGT3VWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
Ca9-22 NG\sfWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTF|OT64N|Mh|ryP NFzpN3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
HCT-116 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LFc2lEPTB;MUO3MlIyPyEQvF2= NITM[G49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
LB2518-MEL NGL5WWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LBUmlEPTB;MUO1Mlk6OyEQvF2= MorlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
U-87-MG M3vJOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrXTWM2OD1zM{OuOlA1KM7:TR?= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
NCI-H2087 NXPDcYhiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPETWM2OD1zM{GuOFg{KM7:TR?= NUXtU4lZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
NCI-H1651 M2PGPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTF|MT6yOFMh|ryP MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
A2780 NHzOSIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHnTWM2OD1zMkiuPVg5KM7:TR?= MoHKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
BALL-1 MmOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTF{Nj6yN{DPxE1? NFjUdo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
COLO-679 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmP0TWM2OD1zMk[uO|U{KM7:TR?= NHrIWII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
SF268 M1P1WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDUeZZKSzVyPUGyOk4yPThizszN NXjBUmU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
SN12C NYnpWWdRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\jdmlEPTB;MUK0MlE4PiEQvF2= MmfPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NCI-H1770 NGTHZYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfLXmZKSzVyPUGyOU42OTRizszN MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
NCI-H2452 NEG5WGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;DPWlEPTB;MUKyMlIyKM7:TR?= MnTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
SK-MEL-30 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXhTWM2OD1zMkOuNlQ1KM7:TR?= NXzlR3JTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
ML-2 M4nDdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HSVmlEPTB;MUKxMlY4PiEQvF2= M4KwblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SW1990 NWPzNnFZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYeyRVFvUUN3ME2xNlAvQTVzIN88US=> NUK3c4NzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
D-392MG MmH2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{XDWmlEPTB;MUG3MlM6QCEQvF2= Mn;xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NCI-H1793 NG\1dYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DxRmlEPTB;MUC5MlI5KM7:TR?= NH\6TIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
GOTO Mn3WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHtNZdXUUN3ME2xNFcvPzd5IN88US=> NULqOJZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
HLE M4fwb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLITWM2OD1zMEWuNlgzKM7:TR?= NInvbVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NCI-H209 Mm[2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPITWM2OD1zMECuNVIyKM7:TR?= Mne2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
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SBC-1 M4\XSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHXTWM2OD1yLkS0NFM2KM7:TR?= M1\sclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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H9 MnuwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTBwM{i4PFMh|ryP NV3VVXVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
NCI-H720 NV\r[JVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvpNW1KSzVyPUCuN|c3PzlizszN NEj0b3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
KARPAS-45 NWLYV4p2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PiV2lEPTB;MD6zO|MzOSEQvF2= MknXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
RS4-11 MorlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LSdmlEPTB;MD6zOFYyKM7:TR?= NVjJ[WxZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
ES5 NHiwWm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfXT3RGUUN3ME2wMlM1PDV3IN88US=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
MOLT-16 NGXSOHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFOwU2JKSzVyPUCuN|A{OiEQvF2= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
HH NVyyUYt2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmToTWM2OD1yLkOwNlg{KM7:TR?= NF\zZYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
T47D NWeyNIFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGL4SFRKSzVyPUCuNlgzPSEQvF2= NUPxc|RMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
AsPC-1 NHrEfpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTBwMkKxNlIh|ryP NEW5cYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
MDA-MB-175-VII MoHES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2KwNmlEPTB;MD6xN|c{QCEQvF2= M1ryNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
P12-ICHIKAWA NYfkS|lGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{[0dmlEPTB;MD6xNVYzKM7:TR?= Mn;kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NEC8 M3H6e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTBwMEm2OVEh|ryP M4\vRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
HONE-1 NF3XenpHfW6ldHnvckBCe3OjeR?= M2Dhb|ExKM7:TR?= NUG3cWVqPDhiaB?= NF3nbFFFVVOR M4nIeWlv\HWlZYOgZ4VtdCCleXPs[UBienKnc4SgZZQhTzF? NGTpRnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOzOlkzPSd-MkWzN|Y6OjV:L3G+
CNE-2 NYHuWHRmTnWwY4Tpc44hSXO|YYm= M{XsZlExKM7:TR?= NGTxc|c1QCCq MlX4SG1UVw>? NXL5VXBPUW6mdXPld{Bk\WyuIHP5Z4xmKGG{cnXzeEBifCCJMR?= NWTXUm5ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzN|Y6OjVpPkK1N|M3QTJ3PD;hQi=>
HONE-1 NX\0OWlET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jFTVExKM7:TR?= MkT3PVYhcA>? M{P6bmROW09? MofWTWM2OD1|LkO3JO69VQ>? NYToc5d6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzN|Y6OjVpPkK1N|M3QTJ3PD;hQi=>
SUNE-1 NUWwc5RVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3WNVAh|ryP NGXoSZE6PiCq NXLrboRVTE2VTx?= MV\JR|UxRTBwNUKg{txO NGr0bYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOzOlkzPSd-MkWzN|Y6OjV:L3G+
CNE-2 NYLjZ2FQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXqxNEDPxE1? Mk[3PVYhcA>? NYr6RoNKTE2VTx?= MlG5TWM2OD12LkWzJO69VQ>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN|NkmyOUc,OjV|M{[5NlU9N2F-
CNE-1 M1rTeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWOxNEDPxE1? M3HvXFk3KGh? M4KxcGROW09? M{S0fWlEPTB;Mj65OkDPxE1? NIruO5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOzOlkzPSd-MkWzN|Y6OjV:L3G+
GEO NVW0dFdXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInmR2w2ODBibl2= NXTtOHA{PzJiaB?= MXrEUXNQ M2fTS2lvcGmkaYTzJINmdGxiZ4Lve5Rp NInzfVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW4NVI{OSd-MkS1PFEzOzF:L3G+
HepG2 cell NXfTToRiSXCxcITvd4l{KEG|c3H5 NFX0VnYzOCEQvF2= M3jBXFI1KGh? M2WxV2ROW09? MmO4TY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7N{OxPUc,OjN5OUezNVk9N2F-
HepG2 cell NULpd2xbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnzUWdsOzBizszN Ml;rNlQhcA>? M3nPO2ROW09? MULJcohq[mm2czDj[YxtKGe{b4f0bC=> M33VNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{m3N|E6Lz5{M{e5O|MyQTxxYU6=
HepG2 cell NWrJdYRuU2mwYYPlJGF{e2G7 MkTsNlAh|ryP M3\2fVI1KGh? NF7DcldFVVOR M2fsUWRwf26{ZXf1cIF1\XNidHjlJJBpd3OyaH;yfYxifGmxbjDv[kBCU1R? M3PX[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{m3N|E6Lz5{M{e5O|MyQTxxYU6=
CEM-S M1vYfGZ2dmO2aX;uJGF{e2G7 MUm05qCK|ryP M1j6PFQhcA>? NWLFWWx2TE2VTx?= MVrJcoNz\WG|ZYOgeIhmKGGvb4XueEBw\iClbHXheoVlKEyFNVGvRkwh[SC5ZXzsMYV{fGGkbHnzbIVlKGG3dH;wbIFogSCvYYLr[ZI> M1mxWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkG0NlQ{Lz5{Mk[xOFI1OzxxYU6=
CEM-R NGjnWIVHfW6ldHnvckBCe3OjeR?= NUG4UmV[POLCid88US=> NGXsflA1KGh? M2rTbmROW09? MWLJcoNz\WG|ZYOgeIhmKGGvb4XueEBw\iClbHXheoVlKEyFNFGvRkwh[SC5ZXzsMYV{fGGkbHnzbIVlKGG3dH;wbIFogSCvYYLr[ZI> NUmzfoVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVQzPDNpPkKyOlE1OjR|PD;hQi=>
MOLT-4 NGX0NlFHfW6ldHnvckBCe3OjeR?= NV7lc456POLCid88US=> MWS0JIg> MoHiSG1UVw>? Mn7XTY5kemWjc3XzJJRp\SCjbX;1cpQhd2ZiY3zlZZZm\CCOQ{PBM2ItKGFid3XscE1me3SjYnzpd4hm\CCjdYTvdIhi\3libXHyb4Vz NVv4b2JTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVQzPDNpPkKyOlE1OjR|PD;hQi=>
MOLT-4 MoLJSpVv[3Srb36gRZN{[Xl? MWWxNEDPxE1? MonhNlQhcA>? MX3EUXNQ MonpRoxw[2u|IHPlcIx{KGmwIITo[UBIOC:JMTDwbIF{\SCxZjD0bIUh[2WubDDjfYNt\Q>? NVXV[VBkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVQzPDNpPkKyOlE1OjR|PD;hQi=>
CEM-S NXm3N5pqS3m2b4TvfIlkKEG|c3H5 MmL3NVAh|ryP MkjBOFghcA>? MVrEUXNQ M2e4XmlEPTB;NT6x5qCK|ryP NGq1Opc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[xOFI1Oyd-MkK2NVQzPDN:L3G+
CEM-R M1;lOmN6fG:2b4jpZ{BCe3OjeR?= NEDKUIsyOCEQvF2= NFy0RnE1QCCq M33JU2ROW09? NHfufWVKSzVyPUOuN-KBkc7:TR?= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZzNEK0N{c,OjJ4MUSyOFM9N2F-
MOLT-4 M4PkeWN6fG:2b4jpZ{BCe3OjeR?= NE\vWFgyOCEQvF2= NV3TWm1lPDhiaB?= NYfEWXVJTE2VTx?= NGTtWpNKSzVyPUGuO-KBkc7:TR?= MnrwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4MUSyOFMoRjJ{NkG0NlQ{RC:jPh?=
HC11 M1WwPGZ2dmO2aX;uJGF{e2G7 MXyxNEDPxE1? MnWzNlQhcA>? MUXEUXNQ NXm0OW9zUW6qaXLpeJMh|rJvY3Hz[YlvKGGwZDDBSHJRKHO7boTo[ZNqew>? NF64b2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOlYzOSd-MkK0NlY3OjF:L3G+
T98G NGTxV3ZCeG:ydH;zbZMhSXO|YYm= NGPVTlkxNjVizszN Ml\ROlAhcA>? MlrnSG1UVw>? MkLORZVodWWwdIOgZZBweHSxZ3XubYMh\W[oZXP0d{Bw\iCpZX\peIlvcWJ? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB3N{mxOEc,OjJyNUe5NVQ9N2F-
TAD2 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXmxNFAxKM7:TR?= NXq5eFNSPSCm M4GzR2ROW09? MY\JR|UxRjFyMECg{txO M37D[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
LN229 MnvoRZBweHSxc3nzJGF{e2G7 NX3FdG5JOC53IN88US=> NV:wOHd5PjBiaB?= M3nCUmROW09? NEfse5RCfWevZX70d{BieG:ydH;n[Y5q[yCnZn\lZ5R{KG:oIHfl[ol1cW6rYh?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB3N{mxOEc,OjJyNUe5NVQ9N2F-
WRO NVvvWJFzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLSOo94OTByMDFOwG0> NULVN5pNPSCm MlfxSG1UVw>? NWK1V5R[UUN3ME6xNFAxKM7:TR?= NEO0VWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK4PVI3Pyd-MkGyPFkzPjd:L3G+
SW1736 NYHvZo1XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvGXWEyODBizszN MXK1JIQ> NYL3e45pTE2VTx?= M1PIWmlEPTB;NEeuOVYh|ryP M{nZTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
KAT18 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnTXN{DPxE1? M160NlUh\A>? M2W0cmROW09? NYjjeVZDUUN3ME20MlYzKM7:TR?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ6OUK2O{c,OjF{OEmyOlc9N2F-
Hth74 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDIeGk{KM7:TR?= NYDiW3ZjPSCm NHzLUmhFVVOR MYPJR|UxRTJwMUmg{txO M3HNfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
TPC1 cells harbored RET/PTC1 rearrangement MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULnPXJQOyEQvF2= M2fPUFUh\A>? MnvqSG1UVw>? NVLuVmpWUUN3ME2wMlU6KM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ6OUK2O{c,OjF{OEmyOlc9N2F-
Hth7 cells harbored NRAS (Q61R+/−) MoTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nYWVMh|ryP MVm1JIQ> NV\4bm1nTE2VTx?= MkTqTWM2OD12LkWg{txO MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ6OUK2O{c,OjF{OEmyOlc9N2F-
C643 cells harbored HRAS (G13R+/−) MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1SyV|Mh|ryP MVO1JIQ> NF7KV4NFVVOR NIHNNnFKSzVyPUCuNlch|ryP MkXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OEmyOlcoRjJzMki5NlY4RC:jPh?=
FTC133 cells harbored PTEN (allele deletion and R130+) MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDRTnM{KM7:TR?= M3v1bFUh\A>? MWDEUXNQ MXvJR|UxRTBwMUig{txO M2rQN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
K1 cells harbored PIK3CA (E542K+/+) Ml64S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nqPFMh|ryP NIXNT4E2KGR? M17nNWROW09? MXjJR|UxRTBwNUKg{txO Mn\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OEmyOlcoRjJzMki5NlY4RC:jPh?=
OCUT1 cells harbored PIK3CA (H1047R+/+) M{TCR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3WydFMh|ryP MVi1JIQ> MlrCSG1UVw>? NXjZ[pdGUUN3ME2wMlE1KM7:TR?= NYGzSVc4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPFkzPjdpPkKxNlg6OjZ5PD;hQi=>
Sk-Hep1 MXvDfZRwfG:6aXOgRZN{[Xl? MXixNEDPxE1? NYTq[XJnOjRiaB?= M3f2V2ROW09? M4\QOnNmdnOrdHn6[ZMhemW|aYP0ZY51KGOnbHzzJJRwKHSqZTDjfZRwfG:6aXOg[YZn\WO2IH;mJJNwemGoZX7pZi=> NUfSRmdTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyNFU6OjVpPkKxNlA2QTJ3PD;hQi=>
HepG2 NUPvWGVvS3m2b4TvfIlkKEG|c3H5 NFTKfHcyOCEQvF2= NUHOOVlQOjRiaB?= MYrEUXNQ M1Tkd3NmdnOrdHn6[ZMhemW|aYP0ZY51KGOnbHzzJJRwKHSqZTDjfZRwfG:6aXOg[YZn\WO2IH;mJJNwemGoZX7pZi=> MlTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{MEW5NlUoRjJzMkC1PVI2RC:jPh?=
A431 NHvCUpBMcW6jc3WgRZN{[Xl? NXj6NWoyPSEQvF2= MX21JIg> NGLEXphFVVOR MUHTeZBxemW|c3XzJJRp\SC|aXfuZYxqdmdib3[gRYt1KGGwZDDFdos> M1zMWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUexNFY6Lz5{MEW3NVA3QTxxYU6=
NCI-H292 M3zMT2N6fG:2b4jpZ{BCe3OjeR?= MXqzJO69VQ>? NGfHZ|E4OiCq MmXCSG1UVw>? NInOV5VKdmirYnn0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbh?= NVviWmJORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1O|ExPjlpPkKwOVcyODZ7PD;hQi=>
HeLa M3vCUGZ2dmO2aX;uJIF{e2G7 M3e3e|EhcA>? NHXFUXBKSzVyIE2gNE4xOjJ6IN88US=> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTFyMEezN{c,OjlzMEC3N|M9N2F-
NCI-H460 Mn;2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVnVNVVRPzJiaB?= M{GzeGlEPTBiPTC1MlQh|ryP M{HLRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwPFYzLz5{NEmwNFg3OjxxYU6=
HCT116 MljlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVzKTHZFPzJiaB?= NWS0VZM1UUN3MDC9JFcvPSEQvF2= MkLvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC4OlIoRjJ2OUCwPFYzRC:jPh?=
A673 MlTYSpVv[3Srb36gZZN{[Xl? NEXxNHFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucx?= MonhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
DAOY Mmr4SpVv[3Srb36gZZN{[Xl? MYfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhTEGRWTDj[Yxtew>? NX7JW|RZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 MVrGeY5kfGmxbjDhd5NigQ>? MmH3dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?= NETXbpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-37 NWTzcW5QTnWwY4Tpc44h[XO|YYm= M3TKcpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2zO{Bk\Wyucx?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
RD MlTFSpVv[3Srb36gZZN{[Xl? M13UfpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>? MlLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH NILCcJNHfW6ldHnvckBie3OjeR?= M3LMTJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMXNJKGOnbHzz M{e1[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-12 NGr4Vm5HfW6ldHnvckBie3OjeR?= NWnHeHpleUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVEzKGOnbHzz MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MG 63 (6-TG R) M{O1VWZ2dmO2aX;uJIF{e2G7 NX\EbYVNeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF3HJFY{KCh4LWTHJHIqKGOnbHzz NWT2cmR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 NV63T5J6TnWwY4Tpc44h[XO|YYm= Mm\CdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= MmjaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
OHS-50 MYrGeY5kfGmxbjDhd5NigQ>? NGjRZ2dyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzz M3rB[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh41 MnrUSpVv[3Srb36gZZN{[Xl? MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? M4jMVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh30 M{\ISWZ2dmO2aX;uJIF{e2G7 NWDRU4Z4eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoN|Ah[2WubIO= Mn\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 M1;hfmZ2dmO2aX;uJIF{e2G7 NInDXlZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1qtS2JOOiClZXzsdy=> NX\CboFyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC NUL0OIpqTnWwY4Tpc44h[XO|YYm= M2HQc5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz M{fiRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1 NWjvRoZKTnWwY4Tpc44h[XO|YYm= M3jZbpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQj3FRoMyKGOnbHzz NW[4XnBkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
LAN-5 NGTmSGlHfW6ldHnvckBie3OjeR?= M{XObZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCOQV6tOUBk\Wyucx?= NYnnO3hURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh18 MoTmSpVv[3Srb36gZZN{[Xl? NWXrTWNYeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoNVgh[2WubIO= MnXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT(T308) / p-AKT(S473) / pGSK3β(S9) / pFOXO1 / pPRAS(T246) / pBAD / pS6K(T389) / p4E-BP1; 

PubMed: 22932669     


ZR75-1 cells were treated with MK-2206 150 nmol/L and collected after indicated hours. Akt signaling was assessed by Western blotting. 

22932669
Growth inhibition assay
Cell viability; 

PubMed: 24665203     


(A) MK-2206 reduced the viability of anaplastic thyroid carcinoma (ATC) cells. CAL62 and KAT4 cells were treated with MK-2206 for 72 hours, followed by measurement with a cell viability assay. (B) In vitro cell viability assay of combination with MK-2206 and tyrphostin AG 1296 in KAT4 cells. 

24665203
Immunofluorescence
LC3; 

PubMed: 24490764     


Immunofluorescence staining of LC3A (green) and DAPI (blue) in M368 cells following treatment with either MK-2206, Carboplatin and Paclitaxel or the combination of all three agents for 72h.

CFTR/LC3; 

PubMed: 28794469     


Co-localisation of CFTR (red- 549nm) and LC3 (green- 488nm) in ΔF508 CFBE41o- cells treated with MK-2206 and a negative control CFBE41o- cells at 37 ℃. Nuclei were stained with DAPI (blue- 358nm). Scale bar is 10 µm.

HMGA2; 

PubMed: 24476133     


Immunofluorescent staining for HMGA2 of leiomyoma cells in control and MK-2206-treated cells was done. Cells were costained with DAPI. Immunointensity of HMGA2 expression was measured and calculated. *, P < .05; **, P < .01; ***, P < .001.

24490764 28794469 24476133
ELISA
VEGFA; 

PubMed: 29449553     


VEGF-A protein expression of conditioned medium was determined by ELISA in cervical cancer cells. The results were analyzed using one-way ANOVA. Column, mean (n = 3); bars, SD. **P < 0.01, compared with control. ##P < 0.01, compared with NF90.

29449553
In vivo MK-2206 shows 60% TGI and inhibits more than 70 % of phospho-Akt1/2 (T308 and S473) in A2780 ovarian cancer xenografts at a dose of 240 mg/kg. [1] MK-2206 exhibits significant antitumor activity in NCI-H292 xenograft in combination with erlotinib or lapatinib. [2]

Protocol

Kinase Assay:

[4]

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Akt kinases assay:

Akt kinases are assayed by a GSK-derived biotinylated peptide substrate. The extent of peptide phosphorylation is determined by Homogeneous Time Resolved Fluorescence (HTRF) using a lanthanide chelate (Lance)-coupled monoclonal antibody specific for the phosphopeptide in combination with a streptavidin-linked allophycocyanin (SA-APC) fluorophore which will bind to the biotin moiety on the peptide. When the Lance and APC are in proximity, a non-radiative energy transfer takes place from the Lance to the APC, followed by emission of light from APC at 655 nm. Working Solution: 100X protease inhibitor cocktail (PIC): 1mg/mL benzamidine, 0.5 mg/mL pepstatin, 0.5 mg/mL leupeptin, 0.5 mg/mL aprotinin; 10X assay buffer: 500 mM HEPES, pH7.5, 1% PEG, 16.6 mM EDTA, 1 mM EGTA, 1% BSA, 20 mM 9-glycerol phosphate; Quench buffer 50 mM HEPES pH 7.3, 16.6 mM EDTA, 0.1% BSA, 0.1% Triton X-100, 0.17 nM labeled monoclonal antibody, 0.0067 mg/mL SA-APC; ATP/MgCl2 working solution: 1X Assay buffer, 1 mM DTT, 1X PIC, 5% glycerol, active Akt; Peptide working solution: 1X Assay buffer, 1 mM DTT, 1X PIC, 5% glycerol, 2 TM GSK biotinylated peptide. The reaction is assembled by adding 16 µL of ATP/MgCl2 working solution to the appropriate wells. MK-2206 or vehicle (1.0 µL) is added followed by 10 µL of peptide working solution. The reaction is started by adding 13 μL of the enzyme working solution and mixing. The reaction is allowed to proceed for 50 min and then stopped by the addition of 60 µL HTRF quench buffer. The stopped reactions are incubated at room temperature for at least 30 min and then read in the instrument.
Cell Research:

[2]

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  • Cell lines: A431, HCC827, NCI-H292, NCI-H358, NCI-H23, NCI-H1299, Calu-6 and NCI-H460 cells
  • Concentrations: 0, 0.3, 1 and 3 μM
  • Incubation Time: 72 or 96 hours
  • Method:

    MK-2206 is dissolved in DMSO as a stock solution and diluted by culture media before use. Cells are seeded at a density of 2-3 × 103 in 96-well plates and incubated for 24 hours. Then MK-2206 (0, 0.3, 1 and 3 μM) is added to the cells. Cell proliferation is determined after 72 or 96 hours.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: SK-OV-3, NCI-H292, HCC70, PC-3, and NCI-H460 models in male CD1-nude mice
  • Dosages: 120 mg/kg
  • Administration: Orally administered
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL (29.14 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5%Tween80+ 50%H2O
For best results, use promptly after mixing.
0.625 mg/ml

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 480.39
Formula

C25H21N5O.2HCl

CAS No. 1032350-13-2
Storage powder
in solvent
Synonyms N/A
Smiles C1CC(C1)(C2=CC=C(C=C2)C3=C(C=C4C(=N3)C=CN5C4=NNC5=O)C6=CC=CC=C6)N.Cl.Cl

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