MK-2206 2HCl

For research use only.

Catalog No.S1078

1275 publications

MK-2206 2HCl Chemical Structure

CAS No. 1032350-13-2

MK-2206 2HCl is a highly selective inhibitor of Akt1/2/3 with IC50 of 8 nM/12 nM/65 nM in cell-free assays, respectively; no inhibitory activities against 250 other protein kinases observed. MK-2206 2HCl induces autophagy and apoptosis in cancer cells. Phase 2.

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Selleck's MK-2206 2HCl has been cited by 1275 publications

Purity & Quality Control

Choose Selective Akt Inhibitors

Biological Activity

Description MK-2206 2HCl is a highly selective inhibitor of Akt1/2/3 with IC50 of 8 nM/12 nM/65 nM in cell-free assays, respectively; no inhibitory activities against 250 other protein kinases observed. MK-2206 2HCl induces autophagy and apoptosis in cancer cells. Phase 2.
Features The first allosteric small molecule inhibitor of Akt to enter clinical development.
Targets
Akt1 [1]
(Cell-free assay)
Akt2 [1]
(Cell-free assay)
Akt3 [1]
(Cell-free assay)
8 nM 12 nM 65 nM
In vitro

MK-2206 is an allosteric inhibitor and is activated by the pleckstrin homology domain. MK-2206 inhibits auto-phosphorylation of both Akt T308 and S473. MK-2206 also prevents Akt-mediated phosphorylation of downstream signaling molecules, including TSC2, PRAS40 and ribosomal S6 proteins. [1] MK-2206 inhibits Ras wild-type (WT) cell lines (A431, HCC827, and NCI-H292) more potently when compared to Ras-mutant cell lines (NCI-H358, NCI-H23, NCI-H1299, and Calu-6). MK-2206 also shows synergistic responses in combination with cytotoxic agents such as erlotinib or lapatinib in lung NCI-H460 or ovarian A2780 tumor cells. [2] MK-2206 or siRNA-mediated Akt inhibition strongly activates autophagy in human glioma cells. However, eukaryotic elongation factor-2 (eEF-2) silencing suppresses MK-2206-induced-autophagy, with a promotion of apoptotic cell death. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H69 NILpcllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTR2MT6wPFMh|ryP NFnzNIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
HCC38 NFjQO2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{j1VGlEPTB;NECxMlU6OyEQvF2= MkOwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NCI-H2052 NHvufpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTN7OD60O|Ih|ryP M2HwUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
NB17 NEDjdY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvvU45lUUN3ME2zPVIvPTl4IN88US=> Mk\vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
SBC-5 NH\rUnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV21Rpc2UUN3ME2zPFYvQTh3IN88US=> NFrPT5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
K-562 MnTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHvW|U6UUN3ME2zPFMvOzZizszN M4DqcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
T84 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4G2T2lEPTB;M{e0MlcyOiEQvF2= M1LKcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
LU-135 M16wTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTN4Nz6wN|Uh|ryP Mo\FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
TE-9 Ml7XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{P3eGlEPTB;M{[zMlU5QSEQvF2= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
IST-SL1 NFzQfXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnv4TWM2OD1|NEeuOFAyKM7:TR?= M{DjUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
BE-13 MmriS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2K3ZWlEPTB;M{S0MlE3PyEQvF2= Mn\iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NCI-H1993 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTN2Mj6zOlUh|ryP NUXDRolmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
MOLT-13 M4L2eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXm2PGQyUUN3ME2zOFIvOzJ4IN88US=> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
NCI-H630 NHTOcIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEG2VHdKSzVyPUOzNk4zQTRizszN NI\TeGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
KP-N-YN NXP4U5dLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETpVotKSzVyPUO0NU4yOjNizszN MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
C3A MlHCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzxTWM2OD1|MUGuPFAzKM7:TR?= M1vQU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
HEL MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTNyOT65PFMh|ryP NH;BNVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NCI-H810 M33tN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTNzMD61O{DPxE1? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
LU-65 NWTwcGdsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfOfJFKSzVyPUOwOE4{OiEQvF2= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
L-428 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PHN2lEPTB;Mkm3MlYyPiEQvF2= NHHXdG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
FADU M3ThOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTJ7Nz61NUDPxE1? M{CwRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
IGR-1 NVzJOFdCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHZTWM2OD1{OUWuOlU6KM7:TR?= M1TWVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
HDLM-2 MlHQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGm4Z41KSzVyPUK5OE41ODlizszN MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
C8166 NVzLd5VoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTJ5OD61NFYh|ryP MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
TGBC11TKB M3TPNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\MTWM2OD1{N{iuNVc5KM7:TR?= NU\NfItwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
RERF-LC-MS NW\ZZWVTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDE[It{UUN3ME2yO|YvODB5IN88US=> NHWwOIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
COLO-680N Mlv5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LNNmlEPTB;MkeyMlUzPyEQvF2= NYm1NG9yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
NB6 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTJ5MD6xJO69VQ>? NHGzUmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
GAK NYnidWVJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTJ3Mz6wNFIh|ryP Mlr0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
RPMI-8866 M3jtU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHoXo9KSzVyPUK0PU4zPyEQvF2= NFfEcY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NCI-H2347 NY\rfJJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLlTWM2OD1{NEeuO|E{KM7:TR?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
LS-1034 MlfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTJ2Nj6yOlYh|ryP Mn7jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
SH-4 MoPjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\ETJFKSzVyPUK0Ok4xQSEQvF2= NGX3fIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
CCRF-CEM NE\0fWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTJ2Mz63PVUh|ryP NUDlUZRtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
C2BBe1 NXLNZWRTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVy0dmNFUUN3ME2yN|EvQTB3IN88US=> NYT1TmVXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
COLO-320-HSR NE\BOWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFu5Z|hKSzVyPUKxOk4yOjVizszN MlPQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
KNS-42 NGrSe|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWG3e|JYUUN3ME2yNVYvPTd2IN88US=> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
RCM-1 MmrtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHqz[XpKSzVyPUKxOE44PjJizszN M1Tvb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
DBTRG-05MG NF\GR5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\pTWM2OD1{MEeuPVA6KM7:TR?= M1X1UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SW982 MlTKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfHOJpKSzVyPUKwO{46PDhizszN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
SW1116 NX;GUldXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrKZppKSzVyPUKwO{4xPzdizszN MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
HuCCT1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjOfY17UUN3ME2yNFQvOjB6IN88US=> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
NCI-H650 NIDuN5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGD3[YdKSzVyPUKwNk4yODNizszN M17xVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
COLO-678 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\kS2lEPTB;MkCxMlQ2KM7:TR?= NIjkWHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
HT-29 MlzVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DmRWlEPTB;MkCxMlIyOiEQvF2= M4Wxd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SCC-9 NECwSnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfXTWM2OD1zOUGg{txO MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
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NCI-SNU-5 M{Tsc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnL5TWM2OD1zOE[uPFQh|ryP NHSycmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
CAL-72 MmmyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTF6NT6wOVQh|ryP MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
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MDA-MB-231 M{n5VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;YdpBJUUN3ME2xO|cvPTB|IN88US=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
HCE-4 NFjkSo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1T6PGlEPTB;MU[2Mlg1PSEQvF2= NFHib5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
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SAS NEX0dVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4LHfWlEPTB;MU[yMlY4QCEQvF2= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
Capan-2 M4Dm[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLuTWM2OD1zNkCuOVU{KM7:TR?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
KU812 M3fBS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3[zZ2lEPTB;MUW4MlY4PCEQvF2= NInJRnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NCI-H1304 M1\vUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2P2VGlEPTB;MUW0MlY6PCEQvF2= M3PyVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
CAPAN-1 Mo\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYHjcYpPUUN3ME2xOVMvODZ2IN88US=> NWXETHNwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
BEN NHy3doVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUG1R|JMUUN3ME2xOVMvQTJ6IN88US=> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
KYSE-450 NWrH[VNbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTF3MT61N|kh|ryP MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
GT3TKB NVXqcHZxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7wTWM2OD1zNEmuPVI5KM7:TR?= M4jCZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SK-MEL-3 NIPGV|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfDTWM2OD1zNEeuPFc5KM7:TR?= NXixNWFkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
OVCAR-8 NILuTIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nXNWlEPTB;MUS1MlY{PiEQvF2= MlvEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
CP50-MEL-B NIr3OllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLZeVJKSzVyPUG0OE42ODFizszN MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
COR-L88 MkG3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTVTWM2OD1zNEKuNVQh|ryP Mn;oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
Ca9-22 MoLjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlL1TWM2OD1zM{muPFM{KM7:TR?= Mo\BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
HCT-116 M1zjWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nuZWlEPTB;MUO3MlIyPyEQvF2= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
LB2518-MEL M4HvR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTF|NT65PVMh|ryP MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
U-87-MG M3HjOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHrTWM2OD1zM{OuOlA1KM7:TR?= M{TV[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
NCI-H2087 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnFZVdKSzVyPUGzNU41QDNizszN NXfyVIR2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
NCI-H1651 MmXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rMbmlEPTB;MUOxMlI1OyEQvF2= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
A2780 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHr2[41KSzVyPUGyPE46QDhizszN MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
BALL-1 M{PiTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXmXFRIUUN3ME2xNlYvOjNizszN M1y0dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
COLO-679 M2q0bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\HO4p2UUN3ME2xNlYvPzV|IN88US=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
SF268 NHzoV49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTF{Nj6xOVgh|ryP M3naZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SN12C M3\nW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTF{ND6xO|Yh|ryP M3jONlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
NCI-H1770 M4nxZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zjWmlEPTB;MUK1MlUyPCEQvF2= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
NCI-H2452 NV6wUWhzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTF{Mj6yNUDPxE1? NXKzVmNoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
SK-MEL-30 M{W0[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFi0WohKSzVyPUGyN{4zPDRizszN M2fzd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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SW1990 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TXe2lEPTB;MUKwMlk2OSEQvF2= NY\acW9RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
D-392MG M{\nd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M373[2lEPTB;MUG3MlM6QCEQvF2= NFLCUVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NCI-H1793 M3fIeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\lTWM2OD1zMEmuNlgh|ryP NYDGSHBMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
GOTO MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnZTWM2OD1zMEeuO|c4KM7:TR?= NXP0XWtMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
HLE NHrqN2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnewTWM2OD1zMEWuNlgzKM7:TR?= NEnCWo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NCI-H209 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPMV4pKSzVyPUGwNE4yOjFizszN NETnSGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
SW684 MmTOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrQPGRKSzVyPUm4MlY3PTRizszN MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
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SBC-1 M3rwd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;6THlKSzVyPUCuOFQxOzVizszN MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
EFM-19 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;QTWM2OD1yLkS0NFEh|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
H9 MmiyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPDWVNKSzVyPUCuN|g5QDNizszN NH7WT2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NCI-H720 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTBwM{e2O|kh|ryP M1;TNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
KARPAS-45 NUnnbY5ST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkT3TWM2OD1yLkO3N|IyKM7:TR?= M1vkXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
RS4-11 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zIbGlEPTB;MD6zOFYyKM7:TR?= NWrwfVFvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
ES5 Moj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;2WWlEPTB;MD6zOFQ2PSEQvF2= NWrQc4dCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
MOLT-16 M12ydWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTBwM{CzNkDPxE1? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
HH NVnhZY5UT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmO0TWM2OD1yLkOwNlg{KM7:TR?= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
T47D M2fIXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvoTWM2OD1yLkK4NlUh|ryP NH\C[Xg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
AsPC-1 M37QU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzJTWM2OD1yLkKyNVIzKM7:TR?= NIDDOGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
MDA-MB-175-VII NX;BVoFNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTBwMUO3N|gh|ryP M1PVelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
P12-ICHIKAWA M{SxSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTBwMUG2NkDPxE1? MkexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NEC8 M4Sx[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFHzZ|RKSzVyPUCuNFk3PTFizszN NH[0fpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
HONE-1 MUnGeY5kfGmxbjDBd5NigQ>? NGDUe|kyOCEQvF2= MWm0PEBp MnW4SG1UVw>? NX[2TYFkUW6mdXPld{Bk\WyuIHP5Z4xmKGG{cnXzeEBifCCJMR?= MkTGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|M{[5NlUoRjJ3M{O2PVI2RC:jPh?=
CNE-2 Mm\sSpVv[3Srb36gRZN{[Xl? Mk\GNVAh|ryP MlPEOFghcA>? M{TmSGROW09? M{DXRWlv\HWlZYOgZ4VtdCCleXPs[UBienKnc4SgZZQhTzF? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN|NkmyOUc,OjV|M{[5NlU9N2F-
HONE-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3GNVAh|ryP MoHuPVYhcA>? M1K0WGROW09? NVG1SJlvUUN3ME2zMlM4KM7:TR?= MnP4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|M{[5NlUoRjJ3M{O2PVI2RC:jPh?=
SUNE-1 NWrHVpRGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYqxNEDPxE1? MYW5OkBp MU\EUXNQ MYfJR|UxRTBwNUKg{txO MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN|NkmyOUc,OjV|M{[5NlU9N2F-
CNE-2 M2HNW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVH0dnV4OTBizszN NV3HZ4g5QTZiaB?= NYnxdpVQTE2VTx?= M1zyRmlEPTB;ND61N{DPxE1? NEDW[I89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOzOlkzPSd-MkWzN|Y6OjV:L3G+
CNE-1 MoLWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrsWGs6OTBizszN M1WwfFk3KGh? MoDlSG1UVw>? M33FW2lEPTB;Mj65OkDPxE1? M4XWOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{O2PVI2Lz5{NUOzOlkzPTxxYU6=
GEO NVPiRZAxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWq1NFAhdk1? NHfqOZI4OiCq MkDKSG1UVw>? Ml3OTY5pcWKrdIOgZ4VtdCCpcn;3eIg> NUHUTXVvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1PFEzOzFpPkK0OVgyOjNzPD;hQi=>
HepG2 cell M17sPWFxd3C2b4Ppd{BCe3OjeR?= NFu5doUzOCEQvF2= MUSyOEBp M2Lqc2ROW09? MmPDTY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? NHHu[nc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5O|MyQSd-MkO3PVc{OTl:L3G+
HepG2 cell MkXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfEN|Ah|ryP NH3QUHEzPCCq M2\URWROW09? MVjJcohq[mm2czDj[YxtKGe{b4f0bC=> M1TXXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{m3N|E6Lz5{M{e5O|MyQTxxYU6=
HepG2 cell NWnyeoVNU2mwYYPlJGF{e2G7 NXz3dVN2OjBizszN MmjUNlQhcA>? Mn7TSG1UVw>? MmjCSI94dnKnZ4XsZZRmeyC2aHWgdIhwe3Cqb4L5cIF1cW:wIH;mJGFMXA>? NVnUbGtQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVc{OTlpPkKzO|k4OzF7PD;hQi=>
CEM-S NWrUfW9sTnWwY4Tpc44hSXO|YYm= MUK05qCK|ryP NEjsVFk1KGh? NX3ET|FkTE2VTx?= M4jPWWlv[3KnYYPld{B1cGViYX3veY51KG:oIHPs[YF3\WRiTFO1RU9DNCCjIIflcIwu\XO2YXLsbZNp\WRiYYX0c5Bp[We7IH3hdotmeg>? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZzNEK0N{c,OjJ4MUSyOFM9N2F-
CEM-R NVT1WVZSTnWwY4Tpc44hSXO|YYm= MUO05qCK|ryP M1juUFQhcA>? NFPob2ZFVVOR MojnTY5kemWjc3XzJJRp\SCjbX;1cpQhd2ZiY3zlZZZm\CCOQ{TBM2ItKGFid3XscE1me3SjYnzpd4hm\CCjdYTvdIhi\3libXHyb4Vz M2jPZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkG0NlQ{Lz5{Mk[xOFI1OzxxYU6=
MOLT-4 NUnYSHFXTnWwY4Tpc44hSXO|YYm= MWK05qCK|ryP NF\Zc|c1KGh? MVvEUXNQ MUXJcoNz\WG|ZYOgeIhmKGGvb4XueEBw\iClbHXheoVlKEyFM1GvRkwh[SC5ZXzsMYV{fGGkbHnzbIVlKGG3dH;wbIFogSCvYYLr[ZI> NFfOVHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[xOFI1Oyd-MkK2NVQzPDN:L3G+
MOLT-4 M4KxfGZ2dmO2aX;uJGF{e2G7 M3TjdlExKM7:TR?= NFztSYMzPCCq MX\EUXNQ MkW0Roxw[2u|IHPlcIx{KGmwIITo[UBIOC:JMTDwbIF{\SCxZjD0bIUh[2WubDDjfYNt\Q>? NX7QXW5ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVQzPDNpPkKyOlE1OjR|PD;hQi=>
CEM-S M1nhXGN6fG:2b4jpZ{BCe3OjeR?= M1PKZlExKM7:TR?= M1fWR|Q5KGh? MnvMSG1UVw>? MlrLTWM2OD13LkJihKnPxE1? NWq1OYs5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVQzPDNpPkKyOlE1OjR|PD;hQi=>
CEM-R MoTSR5l1d3SxeHnjJGF{e2G7 NF71NGUyOCEQvF2= NGPW[oY1QCCq MYPEUXNQ M1vaeWlEPTB;Mz6z5qCK|ryP M4rQO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkG0NlQ{Lz5{Mk[xOFI1OzxxYU6=
MOLT-4 MYXDfZRwfG:6aXOgRZN{[Xl? NUL1PG9nOTBizszN MYK0PEBp MlmzSG1UVw>? M{jwOGlEPTB;MT635qCK|ryP MmjEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4MUSyOFMoRjJ{NkG0NlQ{RC:jPh?=
HC11 NGXFTWRHfW6ldHnvckBCe3OjeR?= NH;XdGoyOCEQvF2= NEnFfGUzPCCq NFLoR5NFVVOR NGWyUXFKdmirYnn0d{DPui2lYYPlbY4h[W6mIFHEVnAhe3mwdHjld4l{ MlLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2Mk[2NlEoRjJ{NEK2OlIyRC:jPh?=
T98G NU\NfIdbSXCxcITvd4l{KEG|c3H5 Mo\oNE42KM7:TR?= NVP2[YlqPjBiaB?= M1LNeGROW09? MWLBeYdu\W62czDhdI9xfG:pZX7pZ{Bm\m[nY4TzJI9nKGenZnn0bY5q[g>? NIjnPW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC1O|kyPCd-MkKwOVc6OTR:L3G+
TAD2 NXjuXIZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPEcZc4OTByMDFOwG0> M2\MUVUh\A>? NILGVpBFVVOR MX;JR|UxRjFyMECg{txO NHvTWVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK4PVI3Pyd-MkGyPFkzPjd:L3G+
LN229 NHfWWmZCeG:ydH;zbZMhSXO|YYm= M{XVfVAvPSEQvF2= MXG2NEBp MUnEUXNQ MX7BeYdu\W62czDhdI9xfG:pZX7pZ{Bm\m[nY4TzJI9nKGenZnn0bY5q[g>? MmHDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyNUe5NVQoRjJ{MEW3PVE1RC:jPh?=
WRO NG\RVllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfKemhnOTByMDFOwG0> MWG1JIQ> NYe4e2V{TE2VTx?= M1zTUWlEPTB-MUCwNEDPxE1? MlfjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OEmyOlcoRjJzMki5NlY4RC:jPh?=
SW1736 NFLNTHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPpOHhqOTByIN88US=> MXS1JIQ> NIPPOFFFVVOR NULDTVNyUUN3ME20O{42PiEQvF2= NFPXTWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK4PVI3Pyd-MkGyPFkzPjd:L3G+
KAT18 NFjlTIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PCOFMh|ryP NVW1RppGPSCm NEPuVGNFVVOR M3m4T2lEPTB;ND62NkDPxE1? M3\VSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
Hth74 M1;Re2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEOwWlk{KM7:TR?= MmrBOUBl M4PNcWROW09? MXfJR|UxRTJwMUmg{txO MomyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OEmyOlcoRjJzMki5NlY4RC:jPh?=
TPC1 cells harbored RET/PTC1 rearrangement M3HReGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX6zJO69VQ>? NHH1Ums2KGR? M1jCNWROW09? MYrJR|UxRTBwNUmg{txO NYLUNocyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPFkzPjdpPkKxNlg6OjZ5PD;hQi=>
Hth7 cells harbored NRAS (Q61R+/−) MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{L2NVMh|ryP M4j3fVUh\A>? Ml;sSG1UVw>? NF3vd|hKSzVyPUSuOUDPxE1? NGTZWnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK4PVI3Pyd-MkGyPFkzPjd:L3G+
C643 cells harbored HRAS (G13R+/−) MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fh[FMh|ryP Ml7rOUBl NFjBR2ZFVVOR NVKwWW1iUUN3ME2wMlI4KM7:TR?= MoPDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OEmyOlcoRjJzMki5NlY4RC:jPh?=
FTC133 cells harbored PTEN (allele deletion and R130+) MmHTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;lN{DPxE1? MnXlOUBl M1[1UGROW09? NUPydpprUUN3ME2wMlE5KM7:TR?= NVSyZVVuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPFkzPjdpPkKxNlg6OjZ5PD;hQi=>
K1 cells harbored PIK3CA (E542K+/+) MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYq4[pN3OyEQvF2= MU[1JIQ> MWnEUXNQ NVPmeY9UUUN3ME2wMlUzKM7:TR?= NIXpNGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK4PVI3Pyd-MkGyPFkzPjd:L3G+
OCUT1 cells harbored PIK3CA (H1047R+/+) MoDyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfqVYx4OyEQvF2= M1PURVUh\A>? M4\kcmROW09? MlPNTWM2OD1yLkG0JO69VQ>? NYnqOm06RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPFkzPjdpPkKxNlg6OjZ5PD;hQi=>
Sk-Hep1 Mlv3R5l1d3SxeHnjJGF{e2G7 MmfqNVAh|ryP MUWyOEBp M3LveGROW09? MnHjV4Vve2m2aYrld{Bz\XOrc4ThcpQh[2WubIOgeI8hfGinIHP5eI91d3irYzDl[oZm[3Rib3[gd49z[W[nbnni NX\DUIhERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyNFU6OjVpPkKxNlA2QTJ3PD;hQi=>
HepG2 NYTmbo5US3m2b4TvfIlkKEG|c3H5 M2\seVExKM7:TR?= M2L2cFI1KGh? MkHlSG1UVw>? MUTT[Y5{cXSrenXzJJJme2m|dHHueEBk\WyuczD0c{B1cGViY4n0c5RwgGmlIHXm[oVkfCCxZjDzc5Ji\mWwaXK= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJyNUmyOUc,OjF{MEW5NlU9N2F-
A431 NUT3T|ZxU2mwYYPlJGF{e2G7 NXXWcmg3PSEQvF2= M4TCPVUhcA>? NVXGcFZJTE2VTx?= NYHXbIJSW3WycILld5NmeyC2aHWgd4lodmGuaX7nJI9nKEGtdDDhcoQhTXKt NWTzUVhbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1O|ExPjlpPkKwOVcyODZ7PD;hQi=>
NCI-H292 M4PoOmN6fG:2b4jpZ{BCe3OjeR?= MXqzJO69VQ>? MoD2O|IhcA>? MknwSG1UVw>? Mk\FTY5pcWKrdHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25? M{fHOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUexNFY6Lz5{MEW3NVA3QTxxYU6=
HeLa NF3iZmlHfW6ldHnvckBie3OjeR?= MkjiNUBp NWTaTXJHUUN3MDC9JFAvODJ{ODFOwG0> MnTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzMEC3N|MoRjJ7MUCwO|M{RC:jPh?=
NCI-H460 M2\Hcmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlywO|IhcA>? MY\JR|UxKD1iNT60JO69VQ>? NEDqUJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFg3Oid-MkS5NFA5PjJ:L3G+
HCT116 M4nIdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2XyRlczKGh? Mn[yTWM2OCB;IEeuOUDPxE1? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEi2Nkc,OjR7MEC4OlI9N2F-
A673 MXvGeY5kfGmxbjDhd5NigQ>? MWfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? M4TKNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
DAOY M4SxWmZ2dmO2aX;uJIF{e2G7 NHrKRpZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?= MmnyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Saos-2 M3e5bmZ2dmO2aX;uJIF{e2G7 M3TxbZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVYX;zMVIh[2WubIO= M4K0dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 M2PPbGZ2dmO2aX;uJIF{e2G7 M2LzVJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2zO{Bk\Wyucx?= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
RD MWDGeY5kfGmxbjDhd5NigQ>? MkLvdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-SH NVjt[XAxTnWwY4Tpc44h[XO|YYm= NWTIWoZieUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uW0hiY3XscJM> Mm\ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-12 Mm\LSpVv[3Srb36gZZN{[Xl? Mmn0dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUGyJINmdGy| M{PBe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R) NVrleI1uTnWwY4Tpc44h[XO|YYm= MYfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN? M2LSRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 NVmzWIF4TnWwY4Tpc44h[XO|YYm= MYPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| NGfQTWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 MV3GeY5kfGmxbjDhd5NigQ>? MVvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| NFHvOWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh41 MofvSpVv[3Srb36gZZN{[Xl? M4LNTpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaESxJINmdGy| MkWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh30 NH\DfnVHfW6ldHnvckBie3OjeR?= NV7ZcHJjeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoN|Ah[2WubIO= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 NFvYSVVHfW6ldHnvckBie3OjeR?= MlLndWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= M3TjO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC MUTGeY5kfGmxbjDhd5NigQ>? MYrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= MoHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 MXzGeY5kfGmxbjDhd5NigQ>? M{\Q[pFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQj3FRoMyKGOnbHzz MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
LAN-5 NGrYZm5HfW6ldHnvckBie3OjeR?= MlzkdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| NF6ybG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh18 M2m4OWZ2dmO2aX;uJIF{e2G7 NHHFeZFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnixPEBk\Wyucx?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT(T308) / p-AKT(S473) / pGSK3β(S9) / pFOXO1 / pPRAS(T246) / pBAD / pS6K(T389) / p4E-BP1; 

PubMed: 22932669     


ZR75-1 cells were treated with MK-2206 150 nmol/L and collected after indicated hours. Akt signaling was assessed by Western blotting. 

22932669
Growth inhibition assay
Cell viability; 

PubMed: 24665203     


(A) MK-2206 reduced the viability of anaplastic thyroid carcinoma (ATC) cells. CAL62 and KAT4 cells were treated with MK-2206 for 72 hours, followed by measurement with a cell viability assay. (B) In vitro cell viability assay of combination with MK-2206 and tyrphostin AG 1296 in KAT4 cells. 

24665203
Immunofluorescence
LC3; 

PubMed: 24490764     


Immunofluorescence staining of LC3A (green) and DAPI (blue) in M368 cells following treatment with either MK-2206, Carboplatin and Paclitaxel or the combination of all three agents for 72h.

CFTR/LC3; 

PubMed: 28794469     


Co-localisation of CFTR (red- 549nm) and LC3 (green- 488nm) in ΔF508 CFBE41o- cells treated with MK-2206 and a negative control CFBE41o- cells at 37 ℃. Nuclei were stained with DAPI (blue- 358nm). Scale bar is 10 µm.

HMGA2; 

PubMed: 24476133     


Immunofluorescent staining for HMGA2 of leiomyoma cells in control and MK-2206-treated cells was done. Cells were costained with DAPI. Immunointensity of HMGA2 expression was measured and calculated. *, P < .05; **, P < .01; ***, P < .001.

24490764 28794469 24476133
ELISA
VEGFA; 

PubMed: 29449553     


VEGF-A protein expression of conditioned medium was determined by ELISA in cervical cancer cells. The results were analyzed using one-way ANOVA. Column, mean (n = 3); bars, SD. **P < 0.01, compared with control. ##P < 0.01, compared with NF90.

29449553
In vivo MK-2206 shows 60% TGI and inhibits more than 70 % of phospho-Akt1/2 (T308 and S473) in A2780 ovarian cancer xenografts at a dose of 240 mg/kg. [1] MK-2206 exhibits significant antitumor activity in NCI-H292 xenograft in combination with erlotinib or lapatinib. [2]

Protocol

Kinase Assay:

[4]

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Akt kinases assay:

Akt kinases are assayed by a GSK-derived biotinylated peptide substrate. The extent of peptide phosphorylation is determined by Homogeneous Time Resolved Fluorescence (HTRF) using a lanthanide chelate (Lance)-coupled monoclonal antibody specific for the phosphopeptide in combination with a streptavidin-linked allophycocyanin (SA-APC) fluorophore which will bind to the biotin moiety on the peptide. When the Lance and APC are in proximity, a non-radiative energy transfer takes place from the Lance to the APC, followed by emission of light from APC at 655 nm. Working Solution: 100X protease inhibitor cocktail (PIC): 1mg/mL benzamidine, 0.5 mg/mL pepstatin, 0.5 mg/mL leupeptin, 0.5 mg/mL aprotinin; 10X assay buffer: 500 mM HEPES, pH7.5, 1% PEG, 16.6 mM EDTA, 1 mM EGTA, 1% BSA, 20 mM 9-glycerol phosphate; Quench buffer 50 mM HEPES pH 7.3, 16.6 mM EDTA, 0.1% BSA, 0.1% Triton X-100, 0.17 nM labeled monoclonal antibody, 0.0067 mg/mL SA-APC; ATP/MgCl2 working solution: 1X Assay buffer, 1 mM DTT, 1X PIC, 5% glycerol, active Akt; Peptide working solution: 1X Assay buffer, 1 mM DTT, 1X PIC, 5% glycerol, 2 TM GSK biotinylated peptide. The reaction is assembled by adding 16 µL of ATP/MgCl2 working solution to the appropriate wells. MK-2206 or vehicle (1.0 µL) is added followed by 10 µL of peptide working solution. The reaction is started by adding 13 μL of the enzyme working solution and mixing. The reaction is allowed to proceed for 50 min and then stopped by the addition of 60 µL HTRF quench buffer. The stopped reactions are incubated at room temperature for at least 30 min and then read in the instrument.
Cell Research:

[2]

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  • Cell lines: A431, HCC827, NCI-H292, NCI-H358, NCI-H23, NCI-H1299, Calu-6 and NCI-H460 cells
  • Concentrations: 0, 0.3, 1 and 3 μM
  • Incubation Time: 72 or 96 hours
  • Method:

    MK-2206 is dissolved in DMSO as a stock solution and diluted by culture media before use. Cells are seeded at a density of 2-3 × 103 in 96-well plates and incubated for 24 hours. Then MK-2206 (0, 0.3, 1 and 3 μM) is added to the cells. Cell proliferation is determined after 72 or 96 hours.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: SK-OV-3, NCI-H292, HCC70, PC-3, and NCI-H460 models in male CD1-nude mice
  • Dosages: 120 mg/kg
  • Administration: Orally administered
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL (29.14 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5%Tween80+ 50%H2O
For best results, use promptly after mixing.
0.625 mg/ml

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 480.39
Formula

C25H21N5O.2HCl

CAS No. 1032350-13-2
Storage powder
in solvent
Synonyms N/A
Smiles C1CC(C1)(C2=CC=C(C=C2)C3=C(C=C4C(=N3)C=CN5C4=NNC5=O)C6=CC=CC=C6)N.Cl.Cl

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01480154 Active not recruiting Drug: Akt Inhibitor MK2206|Drug: Hydroxychloroquine Advanced Malignant Solid Neoplasm|Stage III Cutaneous Melanoma AJCC v7|Stage III Prostate Cancer AJCC v7|Stage III Renal Cell Cancer AJCC v7|Stage IIIA Cutaneous Melanoma AJCC v7|Stage IIIB Cutaneous Melanoma AJCC v7|Stage IIIC Cutaneous Melanoma AJCC v7|Stage IV Cutaneous Melanoma AJCC v6 and v7|Stage IV Prostate Cancer AJCC v7|Stage IV Renal Cell Cancer AJCC v7 National Cancer Institute (NCI) November 23 2011 Phase 1
NCT01466868 Terminated Drug: MK2206 Diffuse Large B Cell Lymphoma Centre Leon Berard|National Cancer Institute France November 2011 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    What is your recommendation for preparing the solution and how would you deliver it to the mice (i.p. or gavage)?

  • Answer:

    You can resuspend MK-2206 in 15% Captisol at up to 17mg/ml. It's a suspension and is for oral gavage use only.

  • Question 2:

    I would want to know if MK-2206 could cross the blood brain barrier?

  • Answer:

    MK-2206 can cross BBB based on the following reference: https://clinicaltrials.gov/ct2/show/NCT01249105.

Akt Signaling Pathway Map

Akt Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID